KR101154238B1 - Functional Green Tea Beverage - Google Patents

Functional Green Tea Beverage Download PDF

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KR101154238B1
KR101154238B1 KR1020090104340A KR20090104340A KR101154238B1 KR 101154238 B1 KR101154238 B1 KR 101154238B1 KR 1020090104340 A KR1020090104340 A KR 1020090104340A KR 20090104340 A KR20090104340 A KR 20090104340A KR 101154238 B1 KR101154238 B1 KR 101154238B1
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green tea
sewage
barley
functional
rice barley
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KR1020090104340A
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KR20110047635A (en
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최성희
전주연
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동의대학교 산학협력단
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23FCOFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F3/00Tea; Tea substitutes; Preparations thereof
    • A23F3/16Tea extraction; Tea extracts; Treating tea extract; Making instant tea
    • A23F3/30Further treatment of dried tea extract; Preparations produced thereby, e.g. instant tea
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23FCOFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F3/00Tea; Tea substitutes; Preparations thereof
    • A23F3/06Treating tea before extraction; Preparations produced thereby
    • A23F3/14Tea preparations, e.g. using additives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38Other non-alcoholic beverages
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105Plant extracts, their artificial duplicates or their derivatives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L7/00Cereal-derived products; Malt products; Preparation or treatment thereof
    • A23L7/10Cereal-derived products
    • A23L7/197Treatment of whole grains not provided for in groups A23L7/117 - A23L7/196
    • A23L7/1975Cooking or roasting
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00Food ingredients
    • A23V2250/20Natural extracts
    • A23V2250/21Plant extracts
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00Food ingredients
    • A23V2250/20Natural extracts
    • A23V2250/21Plant extracts
    • A23V2250/214Tea
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2300/00Processes
    • A23V2300/14Extraction
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2300/00Processes
    • A23V2300/31Mechanical treatment

Abstract

본 발명은 체중 감소 및 향미 개선 효과가 있는 기능성 녹차 음료에 관한 것으로, 보다 구체적으로는 녹차, 쌀보리 및 하수오를 뜨거운 물에 첨가하여 우려내어 제조 된 기능성 녹차 음료에 관한 것이다. 본 발명에 의하면, 녹차, 쌀보리 및 하수오를 혼합 첨가하여 녹차 음료를 제조함으로써, 체중을 감소시키는 효과가 있는 기능성 음료의 제조가 가능하며, 이로써 비만을 억제하여 당뇨병, 고지혈증과 같은 각종 비만 관련 성인병을 예방할 수 있어 국민 건강 증진의 효과가 있으며, 쌀보리에서 유래되는 구수한 향과 맛에 의해 녹차의 쓴 맛과 떫은 맛을 억제할 수 있어, 우리나라 사람들의 기호에 부합하며, 녹차 음용의 초보자적 사람들의 기호에도 적합하여, 중급녹차의 대중화에도 기여할 수 있다.The present invention relates to a functional green tea drink having an effect of weight loss and flavor improvement, and more particularly to a functional green tea drink prepared by adding green tea, rice barley and sewage to hot water. According to the present invention, by mixing the addition of green tea, rice barley and sewage to produce a green tea beverage, it is possible to manufacture a functional drink having an effect of reducing weight, thereby suppressing obesity to prevent various obesity-related adult diseases such as diabetes, hyperlipidemia It can prevent and improve the health of the people, and the bitter and astringent taste of green tea can be suppressed by the gentle aroma and taste derived from rice barley, which is in line with the taste of Korean people, and it is a sign of the beginners who drink green tea. In addition, it can contribute to the popularization of intermediate green tea.

녹차, 쌀보리, 하수오, 체중, 비만, 향미 Green tea, barley, sewage, weight, obesity, flavor

Description

기능성 녹차 음료{Functional Green Tea Beverage} Functional Green Tea Beverage

본 발명은 체중 감소 및 향미 개선 효과가 있는 기능성 녹차 음료에 관한 것으로, 보다 구체적으로는 녹차, 쌀보리 및 하수오를 뜨거운 물에 첨가하여 우려내어 제조 된 기능성 녹차 음료에 관한 것이다.The present invention relates to a functional green tea drink having an effect of weight loss and flavor improvement, and more particularly to a functional green tea drink prepared by adding green tea, rice barley and sewage to hot water.

경제의 발달과 식생활 및 생활양식의 변화는 콜레스테롤에 의한 고지혈증, 고혈압, 동맥경화와 같은 성인병의 증가를 초래하여 심각한 사회 문제로 대두되고 있다. 이러한 질병의 위험 요인은 식습관, 과체중, 운동 부족, 스트레스, 음주, 흡연 등이 그 원인인 것으로 보고되고 있다. 특히, 동물성 지방의 과다한 섭취로 인한 혈액내의 고 콜레스테롤 혈증과 고 중성지방이 만성 성인병의 발병에 중요한 인자로 여겨지고 있다. Economic development and changes in diet and lifestyle have led to an increase in adult diseases such as cholesterol-related hyperlipidemia, hypertension, and atherosclerosis, which are becoming serious social problems. Risk factors for such diseases are reported to be caused by eating habits, overweight, lack of exercise, stress, drinking, smoking. In particular, hypercholesterolemia and high triglycerides in the blood due to excessive intake of animal fats are considered to be important factors for the development of chronic adult diseases.

한편, 국민들의 건강에 대한 관심이 점점 고조됨에 따라 약용식물이 갖고 있는 다양한 생리활성을 이용하여 각종 성인병을 예방할 수 있는 건강 보조 식품의 개발에 관한 연구가 활발히 진행되고 있다.On the other hand, as the public's interest in health increases, researches on the development of health supplements that can prevent various adult diseases by using various physiological activities of medicinal plants are actively being conducted.

녹차는 동백나무과의 카멜리아 시넨시스(Camellia Sinensis)의 싹이나 잎을 이용하여 차엽(茶葉) 속에 존재하는 산화 효소를 화열(火熱)이나 증기로 처리함으로써 효소를 실활(失活)시켜 제조한 것이다. 녹차는 기원전부터 기호음료로서 음용되어 왔을 뿐만 아니라 근래에는 녹차에 함유된 여러 성분들의 약리적인 메커니즘이 규명됨에 따라 그 가치가 재인식되고 있다. 녹차에는 폴리페놀류인 카테킨, 식이섬유, β-카로틴 카페인, 아미노산, 비타민 C 등의 유효 성분들이 다량으로 함유되어 있어 해독작용, 피로회복, 강심작용, 피부미용에 효과가 우수한 것으로 밝혀졌으며, 특히 녹차에 가장 많이 함유되어 있는 폴리페놀에 의한 항산화작용, 항암작용, 혈중콜레스테롤 저하작용, 항노화작용, 중금속해독작용, 충치예방 및 구취제거 작용 등의 효과들이 입증되면서 크게 주목받고 있다.Green tea is produced by inactivating the enzyme by treating the oxidase present in the tea leaves with heat or steam using the shoots or leaves of Camellia Sinensis of Camellia family. Green tea has not only been consumed as a favorite beverage since BC, but also its value is being recognized as the pharmacological mechanism of various ingredients contained in green tea has recently been identified. Green tea contains a large amount of active ingredients such as polyphenols such as catechin, dietary fiber, β-carotene caffeine, amino acids, and vitamin C. It has been found to be effective in detoxification, fatigue recovery, cardiovascular action and skin beauty. Polyphenols contained in the most antioxidant, anti-cancer, blood cholesterol lowering effect, anti-aging, heavy metal detoxification, caries prevention and bad breath removal effect has been attracting much attention.

쌀보리는 보리의 한 계통으로, 겉보리와 구분하여 부르는 명칭이다. 쌀보리는 일반적으로 겉보리보다 내한성(耐寒性)이 약하므로 대전 이남의 남부지방에서 재배되며, 주로 호남지역의 답리작 재배에서 숙기가 빨라 쌀보리 일변도로 재배되는 데 비해 영남지방에서는 쌀보리보다 겉보리를 많이 재배한다. 쌀보리는 특히 겉보리보다 도정(搗精)이 쉽고 도정수율이 높다.Rice barley is a family of barley and is named after barley. Since barley is generally colder than outer barley, it is cultivated in the southern part of Daejeon, and it is grown as a single side of rice barley due to its early maturation in the cultivation of Honam. . Rice barley is particularly easier to cut than top barley and has a higher yield.

하수오는 중국 원산인 약용식물로서, 뿌리줄기가 땅속으로 벋으면서 군데군데 고구마같이 굵은 덩이뿌리가 생긴다. 원줄기는 가지가 갈라지면서 길게 벋어가고 털이 없다. 잎은 어긋나고 달걀 모양 심장형이며 끝이 뾰족하다. 잎 가장자리는 밋밋하고 턱잎은 원통 모양으로 짧다. 잎자루 밑부분에는 짧은 잎집이 있다. 붉은빛을 띤 갈색 덩이뿌리를 한방에서 하수오라고 하며 강장제?강정제?완하제로 널 리 사용된다.  Sewao is a medicinal plant native to China. Its root stems grow into the ground, forming thick tubers like sweet potatoes. The main stem is long, broken and has no hair. Leaves are alternate, egg-shaped, heart-shaped, pointed. The leaf edge is flat and the chin leaf is short and cylindrical. At the bottom of petiole is short petiole. Reddish brown tubers are called sewage in Chinese medicine and are widely used as tonic, tonic and laxative.

한편, 현재 시판되고 있는 일반 녹차 음료의 경우, 기호성을 높이는데 치중되어 있으며, 건강 증진의 기능성이 강화된 녹차 음료에 대해서는 아직까지 활발한 연구가 이루어지지 않고 있다. 특히, 비만 예방과 관련된 체중 감소의 기능성을 갖추면서 기호성까지 겸비한 녹차 음료에 대해서는 아직까지 알려진 바가 없다.On the other hand, in the case of the commercially available green tea drinks currently focused on improving the palatability, active research on green tea beverages with enhanced functionality of health promotion has not been made yet. In particular, there is no known green tea drink that combines taste with functionality of weight loss associated with the prevention of obesity.

본 발명은 기존의 중급 녹차를 활용하여, 체중을 감소시켜 비만을 예방할 수 있는 기능성 및 향미 개선 효과까지 갖춘 기능성 녹차 음료를 제공하는데 그 목적이 있다.An object of the present invention is to provide a functional green tea drink having a functional and flavor improvement effect that can prevent obesity by reducing the weight by using the existing intermediate green tea.

본 발명은 체중 감소 및 향미 개선 효과가 있는 기능성 녹차 음료에 관한 것으로, 보다 구체적으로는 녹차, 쌀보리 및 하수오를 뜨거운 물에 첨가하여 우려내어 제조 된 기능성 녹차 음료에 관한 것이다.The present invention relates to a functional green tea drink having an effect of weight loss and flavor improvement, and more particularly to a functional green tea drink prepared by adding green tea, rice barley and sewage to hot water.

상기 녹차는 분말 형태로 되어 있는 가루녹차를 사용하는 것이 바람직하며, 상기 가루녹차는 그 재배시기에 따라 상급, 중급, 하급으로 등급이 나뉘며, 본 발명에서 사용되는 녹차의 등급은 특별하게 정해진 것은 아니나, 중급 녹차를 사용하는 것이 바람직하다. 상기 녹차는 그 함량이 특별히 제한되는 것은 아니나, 바람직하게는 물 500ml 당 다엽 5 내지 15g을 가루 내어 첨가하여 우려낸다. The green tea is preferably to use the powdered green tea in powder form, the green tea is divided into a grade, upper, intermediate, lower depending on the cultivation time, the grade of the green tea used in the present invention is not specifically determined It is preferable to use intermediate green tea. The green tea is not particularly limited in its content, but preferably, 5 to 15 g of the multileaf per 500 ml of water is added to the powder to be concerned.

상기 쌀보리는 보리의 한 계통으로, 겉보리와 구분하여 부르는 명칭이다. 쌀보리는 일반적으로 겉보리보다 내한성(耐寒性)이 약하므로 대전 이남의 남부지방에서 재배되며, 주로 호남지역의 답리작 재배에서 숙기가 빨라 쌀보리 일변도로 재배되는 데 비해 영남지방에서는 쌀보리보다 겉보리를 많이 재배한다. 쌀보리는 특히 겉보리보다 도정(搗精)이 쉽고 도정수율이 높다. 한국에서 재배되는 쌀보리의 장려 품종으로는 백동 ?광성(光成) ?목포 51호 ?무안보리 ?논산과 1~6호 ?향천과 1호 등이 있으며, 본 발명에 사용되는 쌀보리의 품종은 어느 품종을 사용하여도 무방하다. 본 발명에서 상기 쌀보리는 그 함량이 특별히 제한되는 것은 아니나, 물 500ml 당 볶음처리 한 쌀보리 10 내지 30g을 첨가하여 우려낸다.The rice barley is a line of barley, and is named separately from outer barley. Since barley is generally colder than outer barley, it is cultivated in the southern part of Daejeon, and it is grown as a single side of rice barley due to its early maturation in the cultivation of Honam. . Rice barley is particularly easier to cut than top barley and has a higher yield. Encouraging varieties of rice barley cultivated in Korea include Baekdong, Kwangseong, Mokpo 51, Muanbori, Nonsan, Nos. 1-6, Hyangcheon and No. 1. You can also use varieties. In the present invention, the content of the barley is not particularly limited, but it is concerned by adding 10 to 30 g of roasted barley per 500 ml of water.

상기 하수오는 중국 원산인 약용식물로서, 뿌리줄기가 땅속으로 벋으면서 군데군데 고구마같이 굵은 덩이뿌리가 생긴다. 원줄기는 가지가 갈라지면서 길게 벋어가고 털이 없다. 잎은 어긋나고 달걀 모양 심장형이며 끝이 뾰족하다. 잎 가장자리는 밋밋하고 턱잎은 원통 모양으로 짧다. 잎자루 밑부분에는 짧은 잎집이 있다. 붉은빛을 띤 갈색 덩이뿌리를 한방에서 하수오라고 하며 강장제?강정제?완하제로 널리 사용된다. The sewage is a medicinal plant that is native to China, and the root stems grow into the ground, where coarse tuberous roots are formed. The main stem is long, broken and has no hair. Leaves are alternate, egg-shaped, heart-shaped, pointed. The leaf edge is flat and the chin leaf is short and cylindrical. At the bottom of petiole is short petiole. Reddish brown tubers are called sewage in Chinese medicine and are widely used as tonic, tonic and laxative.

본 발명에서는 상기 하수오의 함량이 특별히 한정되는 것은 아니나, 바람직하게는 물 500ml 당 수세 후 분쇄처리 한 하수오 2 내지 5g을 첨가하여 우려낸다. In the present invention, the content of the sewage is not particularly limited, but preferably, 2 to 5 g of sewage which is pulverized after washing with water per 500 ml of water is added.

본 발명에 따른 기능성 녹차 음료는, 상기 녹차, 쌀보리 및 하수오를 뜨거운 물에 첨가하여 우려내어 제조한다. 상기 뜨거운 물의 온도는 특별히 제한되는 것은 아니나, 약 80 내지 100℃ 온도범위가 바람직하며, 상기 우려내는 시간은 상기 온도범위의 물에서 약 1분30초~3분 이내로 우려내는 것이 본 발명에 따른 기능성 녹차 음료의 효능, 향미 발휘에 적합하다.Functional green tea beverage according to the present invention is prepared by adding the green tea, rice barley and sewage to hot water. The temperature of the hot water is not particularly limited, A temperature range of about 80 to 100 ° C. is preferred, and the boiling time is about 1 minute 30 seconds to 3 minutes in water in the temperature range, which is suitable for exhibiting the efficacy and flavor of the functional green tea beverage according to the present invention.

본 발명에 의하면, 녹차, 쌀보리 및 하수오를 혼합 첨가하여 녹차 음료를 제조함으로써, 체중을 감소시키는 효과가 있는 기능성 음료의 제조가 가능하며, 이로써 비만을 억제하여 당뇨병, 고지혈증과 같은 각종 비만 관련 성인병을 예방할 수 있어 국민 건강 증진의 효과가 있으며, 쌀보리에서 유래되는 구수한 향과 맛에 의해 녹차의 쓴 맛과 떫은 맛을 억제할 수 있어, 우리나라 사람들의 기호에 부합하며, 녹차 음용의 초보자적 사람들의 기호에도 적합하여, 중급녹차의 대중화에도 기여할 수 있다.According to the present invention, by mixing the addition of green tea, rice barley and sewage to produce a green tea beverage, it is possible to manufacture a functional drink having an effect of reducing weight, thereby suppressing obesity to prevent various obesity-related adult diseases such as diabetes, hyperlipidemia It can prevent and improve the health of the people, and the bitter and astringent taste of green tea can be suppressed by the gentle aroma and taste derived from rice barley, which is in line with the taste of Korean people, and it is a sign of the beginners who drink green tea. In addition, it can contribute to the popularization of intermediate green tea.

이하, 본 발명을 실시예를 통하여 상세히 설명하도록 한다. 하기 실시예는 본 발명을 설명하기 위한 일 예에 지나지 않으며, 이에 의하여 본 발명의 범위가 제한되는 것은 아니다.Hereinafter, the present invention will be described in detail through examples. The following examples are only examples for describing the present invention, and the scope of the present invention is not limited thereto.

<실시예><Examples>

1. One. 쌀보리하수오Rice Barley Sewage 녹차물의Green tea 제조 Produce

중급 녹차((주)장원, 제주) 30g, 반 정도 분쇄한 쌀보리(영천) 20g 및 세척하여 잘게 자른 하수오(성도약업사, 경상북도 봉화군 춘양면) 10g을 뜨거운 물 1L에 첨가하여 1분 30초간 우려내어 쌀보리하수오 녹차물을 제조하였다.30g of intermediate green tea (Changwon, Jeju), 20g of semi-crushed rice barley (Youngcheon) and 10g of washed and chopped sewage (Sungdo Pharmaceutical Co., Ltd., Chunyang-myeon, Bonghwa-gun, Gyeongsangbuk-do) to 1L of hot water Sewage green tea water was prepared.

2. 실험동물의 사육2. Breeding of experimental animals

실험동물은 체중이 평균 192g 되는 약 6주령 수컷 흰쥐(Sprague-Dawley rat, male : 효창 사이언스) 53마리를 들여와, 고형배합사료 ((주) 이레본: 조단백질 20% 이상, 조지방 5% 이상, 조섬유 8.5% 이하, 조회분 7% 이하, 칼슘 0.8% 이상, 인 1% 이하)로 사육하면서 4일간 환경에 적응시켰다. 4일간 안정시킨 후 약 7주령이 되었을 때, 실험동물을 각 군당 8마리씩 6군으로 분리하여 사육 상자에 넣고 물과 상기 고형배합사료로 8주간 사육한 후 실험에 사용하였다. The experimental animals imported 53 Sprague-Dawley rats (male hyochang science) weighing an average of 192 g in weight, and were fed solid blended foods (Irebon Co., Ltd .: 20% or more crude protein, 5% or more crude fat, crude fiber) 8.5% or less, 7% or less of crude ash, 0.8% or more of calcium, 1% or less of phosphorus), and adapted to the environment for 4 days. After stabilization for 4 days at about 7 weeks of age, the experimental animals were divided into 6 groups of 8 animals in each group and placed in a breeding box for 8 weeks after breeding with water and the solid blended feed.

3. 3. 실험군Experimental group -  - 쌀보리하수오녹차물Rice Barley Sewage Green Tea Water 섭취 Intake

상기 2.에서 사육된 수컷 흰쥐들 중 임의의 8마리를 실험군으로 하여 스테인레스 선 케이지(stainless wire cage)에서 분리 사육하였으며, 사육실의 조건은 온도 20 내지 25℃, 습도는 약 60 내지 70%를 유지시켰다. 식이 섭취량은 매일 정해진 시간 없이 사료와 쌀보리하수오녹차물을 제한 없이 먹도록 하며, 체중은 1주일에 한번 씩 일정한 시간에 측정하였다.Any eight of the male rats bred in 2. were separated and bred in a stainless steel wire cage (stainless wire cage) as an experimental group, the conditions of the breeding room is maintained at a temperature of 20 to 25 ℃, humidity of about 60 to 70% I was. Dietary intake was to be eaten without limit for the daily feed and rice barley sewage green tea without limit, body weight was measured once a week at a certain time.

4. 대조군 - 물 섭취4. Control-Water Intake

상기 2.에서 사육된 수컷 흰쥐들 중 임의의 8마리를 분리하여, 대조군으로 스테인레스 선 케이지(stainless wire cage)에서 사육하였다. 사육실의 조건은 역시 온도 20 내지 25℃, 습도는 약 60 내지 70%를 유지시켰다. 식이 섭취량은 매일 정해진 시간 없이 사료와 물을 제한 없이 먹도록 하며, 체중은 1주일에 한번 씩 일정한 시간에 측정하였다.Any eight of the male rats bred in 2. were isolated and reared in a stainless wire cage as a control. The conditions of the feeding room were also maintained at a temperature of 20 to 25 ° C. and a humidity of about 60 to 70%. Dietary intake was to be eaten without limit for food and water every day without a fixed time, body weight was measured at a regular time once a week.

5. 5. 쌀보리하수오녹차물의Rice barley sewage green tea 체중 감소 효과의 확인 Confirmation of weight loss effect

상기 실험군 및 대조군의 수컷 흰쥐의 체중을 측정하여, 본발명에 따른 실시예인 쌀보리하수오녹차물의 체붕 감소 효과를 확인하려 하였다. 하기 [표 1]은 상기 실험군 및 대조군에서 사육되는 각각의 8마리의 수컷 흰쥐의 체중 변화를 8주간 측정한 데이터이다.By measuring the weight of the male rats of the experimental group and the control group, to determine the effect of reducing the body shelf of rice barley sewage green tea of the embodiment according to the present invention. [Table 1] is the data measured for 8 weeks the weight change of each of the eight male rats bred in the experimental group and the control group.

[표 1] 실험군 및 대조군의 수컷 흰쥐의 체중 변화 (단위: g)Table 1 Body weight change of male rats in experimental and control groups (unit: g)

  실험군
(사료 + 쌀보리하수오녹차물)
Experimental group
(Feed + Rice Barley Sewage Green Tea Water)
대조군
(사료 + 물)
Control
(Feed + water)
6/56/5 262262 254254 214214 250250 265265 250250 209209 263263 259259 276276 209209 255255 260260 264264 208208 253253 6/126/12 316316 320320 275275 312312 314314 315315 283283 312312 299299 297297 271271 301301 309309 292292 278278 304304 6/196/19 299299 372372 271271 364364 309309 365365 278278 343343 340340 349349 316316 341341 365365 342342 328328 331331 6/266/26 388388 399399 356356 402402 389389 402402 383383 396396 364364 371371 352352 369369 400400 383383 367367 397397 7/37/3 424424 398398 389389 432432 404404 432432 405405 430430 392392 379379 378378 399399 410410 417417 406406 438438 7/107/10 446446 439439 398398 447447 422422 449449 426426 454454 404404 422422 403403 410410 434434 432432 418418 460460 7/177/17 462462 474474 410410 470470 452 452 480480 448448 483483 420420 447447 422422 436436 474474 466466 446446 491491 7/237/23 481481 490490 423423 496496 476476 494494 454454 497497 439439 481481 451451 458458 490490 477477 456456 515515

상기 [표 1]에 나타난 바와 같이, 실험군에서 사육된 수컷 흰쥐 8마리의 8주 동안의 평균 체중은 367.172g 이었고, 대조군의 8마리의 수컷 흰쥐의 평균 체중은 389.641g 이었다. 즉, 실험군에서 쌀보리하수오녹차물을 섭취한 수컷 흰쥐의 평균 체중이 물을 섭취시켜 사육한 대조군의 수컷 흰쥐의 평균 체중 보다 22.469g 더 낮았으며, 이는 대조군을 기준으로 36.40%의 체중 감소률을 나타내는 효과가 있음을 의미한다.As shown in Table 1, the average weight of 8 male rats in the experimental group for 8 weeks was 367.172g, the average weight of 8 male rats in the control group was 389.641g. That is, the average body weight of male rats fed rice barley sewage green tea in the experimental group was 22.469g lower than the average body weight of male rats in the control group fed with water, indicating a 36.40% weight loss rate based on the control group. It means that it is effective.

6. 6. 쌀보리하수오녹차물의Rice barley sewage green tea 향미 개선 효과의 확인 Confirmation of flavor improvement effect

본 발명에서 향미 개선 효과는 쌀보리의 휘발성 향기 성분들에 의해 기인하는 바, 본 실험에서는 쌀보리의 휘발성 향기성분을 동시증류추출장치(SDE)로 추출하고 GC 와 GC-MS로 분석 동정하였다.In the present invention, the flavor improvement effect is caused by the volatile aroma components of rice barley, in this experiment, the volatile aroma components of rice barley were extracted with a co-distillation extraction apparatus (SDE) and analyzed by GC and GC-MS.

시료로서, 쌀보리는 2008년 영천에서 재배된 것을 볶음 가공한 쌀보리를 구입하여 사용하였으며, 녹차는 (주)장원에서 제조되어 판매되고 있는 제주산 증제녹 차를 구입하여 사용하였다. 볶은 쌀보리와 녹차를 1:1로 혼합하여 쌀보리 녹차 혼합시료를 사용하였고, 대조군으로 볶은 쌀보리 100%와 녹차 100%를 각각 사용하여 쌀보리 녹차 혼합시료의 휘발성 향기 성분 분석용 시료로 사용하였다.As a sample, rice barley was used to purchase roasted barley that was grown in Yeongcheon in 2008, and green tea was used to purchase Jeju-produced green tea, manufactured and sold in Jangwon. Roasted rice barley and green tea were mixed 1: 1 to use the rice barley green tea mixed sample, and as a control, 100% roasted rice barley and 100% green tea were used as samples for analyzing the volatile aroma component of the rice barley green tea mixed sample.

상기 쌀보리가 첨가된 쌀보리 녹차 혼합시료로부터 휘발성 향기성분을 추출하기 위해 Nickerson형 동시증류추출장치(SDE)를 사용하였으며, 추출방법은 분쇄한 시료 각 100g과 증류수 1000ml를 삼구플라스크에 넣고 용매플라스크에는 추출용매인 diethyl ether 50ml를 가한 후 1시간 30분 동안 가열환류하여 휘발성 향기성분을 추출하였다. 추출물은 무수 황산나트륨 (Na2SO4)으로 수분을 제거시킨 후 24시간 동안 보관한 다음 상압(40℃)에서 증류, 농축한 후 diethyl ether 를 제거하여 휘발성 향기성분 농축물을 얻었다.Nickerson type simultaneous distillation (SDE) was used to extract the volatile aroma components from the rice barley green tea mixed sample to which the barley was added. After adding 50 ml of solvent diethyl ether, the mixture was heated to reflux for 1 hour 30 minutes to extract volatile odorant components. The extract was dried with anhydrous sodium sulfate (Na2SO4), stored for 24 hours, distilled and concentrated at atmospheric pressure (40 ° C), and then removed with diethyl ether to obtain a volatile flavor concentrate.

상기 얻어진 휘발성 향기성분 농축물에 대한 휘발성 향기 성분의 분석 및 동정은 기체크로마토그래피(gas chromatography, GC) 및 기체크로마토그래피-질량분석기(Gas chromatography-mass spectrometry, GC-MS)를 이용하여 수행하였다. GC는 shimadzu model GC 17A (Kyoto, Japan)형을 사용하였고, GC-MS는 HP 6890과 HP5973 Mass Selective Detector(Palo, Alto, CA, USA)가 연결된 것을 사용하였으며, 측정 조건은 하기 [표 2]에 나타난 바와 같다. GC에 의해 분리된 각 peak성분은 표준물질의 머무름 시간 (t R ) 및 GC-MS 분석 결과로 얻은 mass spectral library data와 비교하여 확인하였다.Analysis and identification of the volatile scent components of the obtained volatile scent component concentrates were carried out using gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). GC used shimadzu model GC 17A (Kyoto, Japan), GC-MS used HP 6890 and HP5973 Mass Selective Detector (Palo, Alto, CA, USA) is connected, the measurement conditions are shown in [Table 2] As shown in Each peak component separated by GC was confirmed by comparing the retention time (t R ) of the standard and the mass spectral library data obtained by the GC-MS analysis.

[표 2] GC 및 GC-MS 기구 분석 조건TABLE 2 GC and GC-MS Instrument Analysis Conditions

GC : GC : Shimadzu model GC-17A Shimadzu model GC-17A Column :Column: HP-5MS(30m × 0.25mm × 0.25㎛ film)HP-5MS (30m × 0.25mm × 0.25㎛ film) Temp. prog. :Temp. prog. : 50 (8min hold) 220℃50 (8min hold) 220 ℃ 2℃/min                   2 ℃ / min Carrier gas : Carrier gas: nitrogennitrogen Column flow :Column flow: 1.0ml/min1.0ml / min GCGC -- MSMS GC :GC: HP 6890 SeriesHP 6890 Series MS :MS: HP 5973 Mass Selective DetectorHP 5973 Mass Selective Detector Column :Column: HP-5MS(30m ×0.25mm ×0.25㎛ film)HP-5MS (30m × 0.25mm × 0.25㎛ film) Temp. prog. :Temp. prog. : same as GC prog.same as GC prog. Carrier gas : Carrier gas: heliumhelium

상기 GC 및 GC-MS에 의하여, 휘발성 향기성분 농축물로부터 휘발성 향기 성분을 분석 및 동정한 결과, 쌀보리의 휘발성 향기 성분으로 총 87종류의 휘발성 향기성분이 분리되었다(하기 [표 3] 참조).As a result of analyzing and identifying the volatile scent component from the volatile scent component concentrate by GC and GC-MS, a total of 87 kinds of volatile scent components were separated as the volatile scent component of rice barley (see Table 3 below).

[표 3] 쌀보리의 휘발성 향기 성분[Table 3] Volatile Flavor Components of Rice Barley

Peak No.Peak No. tR(min)t R (min) CompoundsCompounds Peak No.Peak No. tR(min)t R (min) CompoundsCompounds 1One 1.6481.648 2-Methyl butane2-Methyl butane 1919 3.3343.334 2-Methyl-2-butenal2-Methyl-2-butenal 22 1.8651.865 2,3-Dimethyl butane2,3-Dimethyl butane 2020 3.5573.557 1H-Pyrrole1H-Pyrrole 33 1.9281.928 3-Methyl pentane3-Methyl pentane 2121 3.7403.740 PentanolPentanol 44 1.9971.997 ButanalButanal 2222 3.8313.831 cis-2-Penten-1-olcis-2-Penten-1-ol 55 2.0372.037 2-Methyl furan2-Methyl furan 2323 4.1404.140 2,3-dihydro-4-methyl furan2,3-dihydro-4-methyl furan 66 2.0712.071 Ethyl acetateEthyl acetate 2424 4.2484.248 2-Propyl furan2-Propyl furan 77 2.1852.185 2,3-Dihydro-3-methyl furan2,3-Dihydro-3-methyl furan 2525 4.4884.488 HexanalHexanal 88 2.3392.339 3-Methyl butanal3-Methyl butanal 2626 4.6774.677 Dihydro-2-methyl-3(2H)-FuranoneDihydro-2-methyl-3 (2H) -Furanone 99 2.4192.419 2-Methyl butanal2-Methyl butanal 2727 4.9114.911 4-Methylthiazole4-Methylthiazole 1010 2.4822.482 ThiofuranThiofuran 2828 5.1345.134 Methyl pyrazineMethyl pyrazine 1111 2.5622.562 1-Penten-3-ol1-Penten-3-ol 2929 5.5235.523 FurfuralFurfural 1212 2.7622.762 2-Ethyl furan2-Ethyl furan 3030 5.7925.792 1-Ethyl-1H-pyrrole1-Ethyl-1H-pyrrole 1313 2.8372.837 2.,5-Dimethyl furan2., 5-Dimethyl furan 3131 6.1296.129 2-Methyl-1H-pyrrole2-Methyl-1H-pyrrole 1414 2.9172.917 2,6-Dimethyl furan2,6-Dimethyl furan 3232 6.2556.255 (E)-2-Hexenal(E) -2-Hexenal 1515 3.0543.054 2-Vinyl furan2-Vinyl furan 3333 6.4836.483 Furfuryl alcohol Furfuryl alcohol 1616 3.1633.163 PyrazinePyrazine 3434 7.0327.032 HexanolHexanol 1717 3.2373.237 2-Methyl-1-butanol2-Methyl-1-butanol 3535 8.0728.072 2-Heptanone2-Heptanone 1818 3.2653.265 4-Methyl-2-pentene4-Methyl-2-pentene 3636 8.4048.404 NonanolNonanol

Peak No.Peak No. tR(min)t R (min) CompoundsCompounds Peak No.Peak No. tR(min)t R (min) CompoundsCompounds 3737 8.6788.678 HeptanalHeptanal 6363 21.93221.932 2-Methyl-5-propyl pyrazine2-Methyl-5-propyl pyrazine 3838 9.1529.152 2,5-Dimethyl pyrazine2,5-Dimethyl pyrazine 6464 22.08622.086 (Z,E)-3,5-Octadiene-2-one(Z, E) -3,5-Octadiene-2-one 3939 9.4279.427 Ethyl pyrazineEthyl pyrazine 6565 22.36622.366 Dimethyl-2-vinyl pyrazineDimethyl-2-vinyl pyrazine 4040 9.6049.604 2,3-Dimethyl pyrazine2,3-Dimethyl pyrazine 6666 25.12725.127 2-Methyl-3-(E)-propenyl pyrazine2-Methyl-3- (E) -propenyl pyrazine 4141 10.25510.255 Ethenyl pyrazineEthenyl pyrazine 6767 26.56226.562 2,3-Diethyl-5-methyl pyrazine2,3-Diethyl-5-methyl pyrazine 4242 12.15912.159 (E)-2-heptenal(E) -2-heptenal 6868 26.71026.710 3,5-Diethyl-2-methyl pyrazine3,5-Diethyl-2-methyl pyrazine 4343 12.25012.250 BenzaldehydeBenzaldehyde 6969 26.91026.910 2,3,5-Trimethyl-6-ethyl pyrazine2,3,5-Trimethyl-6-ethyl pyrazine 4444 12.62212.622 5-Methyl furfural5-Methyl furfural 7070 27.38527.385 2,5-Diethyl-3-methyl pyrazine2,5-Diethyl-3-methyl pyrazine 4545 13.30713.307 CitronellolCitronellol 7171 28.49328.493 1-(2-Furanyl methyl)-1H-pyrrole1- (2-Furanyl methyl) -1H-pyrrole 4646 13.97613.976 1-Octen-3-ol1-Octen-3-ol 7272 28.79628.796 2-Acetyl pyrazine2-Acetyl pyrazine 4747 14.63914.639 2-Pentyl furan2-Pentyl furan 7373 29.84229.842 3,5-Dimethyl-2-isobutyl pyrazine3,5-Dimethyl-2-isobutyl pyrazine 4848 14.95914.959 2-Ethyl-6-methyl pyrazine2-Ethyl-6-methyl pyrazine 7474 30.99730.997 3-Phenyl furan3-Phenyl furan 4949 15.15415.154 2-Ethyl-5-methyl pyrazine2-Ethyl-5-methyl pyrazine 7575 31.76831.768 2,6-Diethyl-3,5-dimethyl pyrazine2,6-Diethyl-3,5-dimethyl pyrazine 5050 15.25615.256 Trimethyl pyrazineTrimethyl pyrazine 7676 32.00832.008 2,3-Diethyl-5,6-dimethyl pyrazine2,3-Diethyl-5,6-dimethyl pyrazine 5151 15.39415.394 2-Ethyl-3-methyl pyrazine2-Ethyl-3-methyl pyrazine 7777 33.15233.152 2-Butyl-3-methyl pyrazine2-Butyl-3-methyl pyrazine 5252 15.63915.639 Propyl pyrazinePropyl pyrazine 7878 34.24934.249 5-Pentyl-3h-furan-2-one5-Pentyl-3h-furan-2-one 5353 15.98215.982 (E,E)-2,4-Heptadienal(E, E) -2,4-Heptadienal 7979 34.80334.803 5-Ethoxy methyl furfural5-Ethoxy methyl furfural 5454 16.20516.205 2-Ethenyl-6-methyl pyrazine2-Ethenyl-6-methyl pyrazine 8080 36.02136.021 1H-Indole1H-Indole 5555 16.19416.194 2-Methyl-6-vinyl pyrazine2-Methyl-6-vinyl pyrazine 8181 36.22136.221 (E,Z)-2,4-Decadienal(E, Z) -2,4-Decadienal 5656 16.41716.417 Isopropyl pyranzineIsopropyl pyranzine 8282 37.71237.712 (E,E)-2,4-Decadienal(E, E) -2,4-Decadienal 5757 18.28018.280 Phenyl acetaldehydePhenyl acetaldehyde 8383 39.36439.364 5858 19.45719.457 OctenalOctenal 8484 40.63340.633 5959 20.96620.966 3-Ethyl-2,5-dimethyl pyrazine 3-Ethyl-2,5-dimethyl pyrazine 8585 42.01142.011 6060 21.38321.383 2-Ethyl-3,5-dimethyl pyrazine 2-Ethyl-3,5-dimethyl pyrazine 8686 42.35342.353 6161 21.54921.549 2,6-Diethyl pyrazine2,6-Diethyl pyrazine 8787 47.03447.034 6262 21.70921.709 2,5-Diethyl pyrazine2,5-Diethyl pyrazine

상기 [표 3]에 의하면, 쌀보리의 주요 휘발성 성분은 고소하고 볶은 향을 가지는 33종류의 pyrazine과 pyrrole류 (약 38%를 차지), 그리고 보리를 볶을 때 생성되는 당류의 분해물로써 달콤한 향을 가지는 methyl butanal과 furfural, 5-hepthyl dihydro-2(3H)-furanone, 5-pentyl-2(3H)-furanone 등의 퓨란류(약 29%를 차지)들이 동정되었다. 결과적으로 볶은 쌀보리의 냄새는 구수한 pyrazine류의 향기 성분들과 아몬드 향으로 알려진 benzaldehyde나 pyrrole류 들이 구수한 향에 많은 기여를 한다. 따라서, 중급녹차 및 하수오 혼합물에 볶은 쌀보리를 혼합하여 제조할 시, 볶은 쌀보리의 구수한 향과 맛이 녹차와 잘 조화되어 우리나라 사람들의 기호에 잘 부합할 뿐 만 아니라, 녹차 음용의 초보자적 사람들의 기호에 적합하여, 중급녹차의 대중화에 대한 기여도가 높을 것이다.According to the above [Table 3], the main volatile components of rice barley are 33 kinds of pyrazine and pyrrole (about 38%) having savory and roasted aroma, and a sweet flavor as a decomposition product of saccharides produced when roasting barley. Furans (about 29%) such as methyl butanal, furfural, 5-hepthyl dihydro-2 (3H) -furanone, and 5-pentyl-2 (3H) -furanone were identified. As a result, the smell of roasted barley contributes a lot to the fragrances of salvaged pyrazines and the fragrances of benzaldehyde or pyrrole known as almond aromas. Therefore, when the roasted rice barley is mixed with the mixture of intermediate green tea and sewage, the delicious aroma and taste of the roasted barley is well harmonized with the green tea, which not only matches the taste of Korean people, but also the taste of the beginners of green tea drinking. In addition, the contribution to the popularization of intermediate green tea will be high.

이상에 설명한 바와 같이, 본 발명이 속하는 기술분야의 당업자는 본 발명이 그 기술적 사상이나 필수적 특징을 변경하지 않고서 다른 구체적인 형태로 실시될 수 있다는 것을 이해할 수 있을 것이다. 본 발명의 범위는 상기의 상세한 설명보다는 후술할 특허청구범위에 의하여 나타내어지며, 특허청구범위의 의미 및 범위 그리고 그 등가개념으로부터 도출되는 모든 변경 또는 변형된 형태가 본 발명의 범위에 포함되는 것으로 해석되어야 한다. As described above, those skilled in the art will understand that the present invention can be implemented in other specific forms without changing the technical spirit or essential features. The scope of the present invention is shown by the claims to be described later rather than the detailed description, and all changes or modifications derived from the meaning and scope of the claims and their equivalent concepts are included in the scope of the present invention. Should be.

Claims (8)

a) 녹차, 볶음처리한 쌀보리, 및 하수오를 분쇄하여 혼합 분말을 제조하는 단계; 및a) grinding green tea, sauteed rice barley, and sewage to prepare a mixed powder; And b) 상기 분쇄된 혼합 분말을 80 내지 100℃의 물에 첨가하여 1분 30초 내지 3분 동안 우려내어 쌀보리하수오 녹차물을 제조하는 단계;를 포함하는 것을 특징으로 하는 기능성 녹차 음료의 제조방법.b) adding the pulverized mixed powder to water at 80 to 100 ° C. for 1 minute 30 seconds to 3 minutes to prepare rice barley sewage green tea water; a method of manufacturing a functional green tea beverage comprising the above. 제 1항에 있어서, The method of claim 1, 상기 녹차는 중급녹차인 것을 특징으로 하는 기능성 녹차 음료의 제조방법.The green tea is a method of producing a functional green tea drink, characterized in that the intermediate green tea. 제 1항에 있어서, The method of claim 1, 상기 녹차는 그 첨가량이 물 500ml 당 다엽 5 내지 15g을 가루 내어 첨가하는 것을 특징으로 하는 기능성 녹차 음료의 제조방법. The green tea is a method of producing a functional green tea drink, characterized in that the addition amount of the powder 5 to 15g per 500ml water added. 제 1항에 있어서, The method of claim 1, 상기 쌀보리는 그 첨가량이 물 500ml 당 볶음처리 한 쌀보리 10 내지 30g을 첨가하는 것을 특징으로 하는 기능성 녹차 음료의 제조방법. The rice barley is a method of producing a functional green tea beverage, characterized in that the addition amount of the roasted rice barley 10-30g per 500ml of water. 제 1항에 있어서,The method of claim 1, 상기 하수오는 그 첨가량이 물 500ml 당 수세 후 분쇄처리한 하수오 2 내지 5g을 첨가하는 것을 특징으로 하는 기능성 녹차 음료의 제조방법. The sewage is a method for producing a functional green tea drink, characterized in that the addition amount of the sewage 2 to 5g pulverized after washing the water per 500ml of water. 삭제delete 삭제delete 제1항 내지 제5항 중 어느 한 항의 방법에 의하여 제조된 기능성 녹차 음료.Functional green tea beverage prepared by the method of any one of claims 1 to 5.
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