KR101138909B1 - Inspecting and Operating System for Small Animal - Google Patents

Abstract

The present invention relates to a small animal test and surgical device, and to a short time the process of performing the image observation of the subject, and to a small animal test and surgical device that can provide an optimized condition of the image observation. .
The small animal examination and surgical device includes an anesthesia chamber on one side, a stage on which a metal seat for seating a subject is formed, a vertical pillar for supporting the stage, and a vertical pillar and a lower portion for supporting the stage. A base, a light source for irradiating light to the subject to acquire the image of the subject, a light detector for extracting the reflected light of the subject to obtain an image, and a device controller for storing and analyzing the image of the subject.

Description

Inspection and Surgery Apparatus for Small Animals {Inspecting and Operating System for Small Animal}

The present invention relates to a small animal test and surgical device, and more particularly to a small animal test and surgical device capable of performing the inspection and surgery of the subject and the information storage and analysis.

Molecular imaging is a technique for detecting and imaging molecular and cellular levels of phenomena in animals.

As a method of molecular imaging, a fluorescent protein is introduced into a subject, and a method of sensing and imaging light emitted from the inside of the material by irradiating with a light source having a wavelength corresponding to the fluorescent material. Such a fluorescence image can observe the tissue of the subject in a way that does not sacrifice the subject, but when the transmission power of the fluorescent light source is weak, the image is obtained through surgery on the subject. In this case, when imaging the subjects by surgery, cells or bacteria in combination with fluorescent proteins were injected, and after a certain time, each place where the imaging was performed was performed in a different place. . Accordingly, the time required for the movement of the subject is not small, and there is a problem in monitoring the moment and storing it as an image.

In addition, the conventional image capturing apparatus is a level of simply observing an image of a subject in the process of taking an image and inspecting the subject, and thus has a limitation in analyzing the image.

The present invention has been made to solve the problems of the prior art and the necessity of technology development, and by reducing the time required to move the subject by implementing anesthesia and post-fluorescence imaging of the subject as an integrated device It is an object of the present invention to provide a small animal test and surgical device that can be.

In another aspect, the present invention provides a small animal inspection and surgical apparatus that can be controlled to observe the image in the process of inspecting the subject through the imaging and to efficiently store and analyze the image through a dedicated software Its purpose is to.

In another aspect, the present invention is to provide a device for fixing the subject in the operation of the subject and to maintain the temperature or anesthesia in accordance with the progress of observation and surgery after the fixation.

In order to achieve the above object, the inspection and surgical device for small animals according to the present invention includes an anesthesia chamber on one side, and a stage on which a metal seat for seating a subject is formed, a vertical pillar for supporting the stage, and a vertical pillar. A lower base disposed at the bottom to support the vertical pillar and the stage, a light source for irradiating light to the subject for image acquisition, a light detector for extracting the reflected light of the subject, and an image of the subject; A device control unit for analyzing is provided.

The apparatus controller may include a setting unit for adjusting the captured image, an input unit for operating the setting unit, a display unit for displaying the captured image, and displaying the contents of the setting unit and the input unit.

The setting unit adjusts the resolution, brightness and shutter speed and gain coefficient of the captured image, and captures and records the captured image displayed on the display unit.

The seating table may further include a magnet-like fixture including a magnet and a connection pin extending from the magnet to hook a portion of the subject.

The light source may be formed of a plurality of LEDs each having a unique wavelength band, and may further include a condenser lens for preventing scattering of light emitted from the light source and concentrating light on the subject.

The seating table may further comprise a head anesthesia.

The filter unit may further include a filter unit disposed between the test subject and the light detector and configured to detect light having a specific wavelength emitted from the test subject.

As described above, according to the present invention, it is possible to reduce the time required for the movement of the subject by integrating a place where the subject is anesthetized and operated.

In addition, in the process of irradiating the subject using a light source having a specific wavelength, the subject may be intensively irradiated by using a condenser lens that collects light.

In proceeding with the operation, the operation can be easily performed through a fixing ring that can stably fix the subject in a free position.

In addition, in the process of analyzing the subject, the conditions of image capturing may be set to efficiently analyze the subject, and may be observed and stored in real time.

1 and 2 are a perspective view and a front view showing a small animal inspection and surgical apparatus according to the present invention.
3 is a view showing a condenser lens;
4 and 5 are a side view and a plan view showing a filter unit.
6 and 7 illustrate a magnet-like fixture and its use.
8 is a view showing an example of a display unit of the device control unit according to the present invention.

The terms used in the present invention were selected as general terms as widely used as possible, but in some cases, the terms arbitrarily selected by the applicant are included. In this case, the meanings described or used in the detailed description of the present invention are considered, rather than simply the names of the terms. The meaning should be grasped.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings.

1 and 2 are a perspective view and a front view showing a small animal inspection and surgical apparatus according to the present invention.

1 and 2, the inspection and surgical device for small animals according to the present invention is a stage 100 including a seating table 110 and an anesthetic chamber 120, a light source 400 for irradiating light to the subject ), A light detector 450 for extracting light (fluorescence) of a specific wavelength emitted from the subject, and an apparatus controller 500 for storing and analyzing the image. The stage 100 is supported by the vertical column 200 coupled with the lower base 300.

The stage 100 is a table for monitoring collection and imaging observation of a subject's tissue through experiments and surgery performed on small animals. To this end, the stage 100 may include an anesthesia chamber 120 for anesthesia of a small animal and a seating stand 110 for seating the small animal for examination and surgery.

The seating unit 110 may be formed of a metal material, and may further include a magnet type fixing body 150 for fixing the small animal.

One side of the stage 100 is disposed a light source 400 for irradiating light to the subject. As the light source 400, a light source having a specific wavelength may be selected according to a fluorescent protein injected into a subject. The light source 400 may use a light emitting diode (LED) 410.

In addition, a condenser lens 412 as illustrated in FIG. 3 may be formed on the front surface of the light source 400. 3 is a diagram representing one LED 410 in the light source 400 using a plurality of LEDs (410). The condenser lens 412 may prevent the light emitted from the light source using the LED 410 from being scattered. When only the LED 410 is used as the light source 400, light spreading occurs, thereby degrading efficiency. The condenser lens 412 coupled to the light source 400 may increase efficiency by inducing the light path of the light emitted from the LED 410 to be concentrated on the subject. In addition, the light source 400 may be coupled to the light source head 420 and coupled to both ends of the light source support 250.

The vertical bar 210 may be coupled to one side of the vertical column 200 or the stage 100. The light source support 250 may be implemented in a "c" shape, and the light source 400 may be disposed at both ends of the light source support 250 in the "c" shape. The light source support 250 may be coupled to the vertical bar 210 by a horizontal connection bar 220 arranged at a right angle with the vertical bar 210. The horizontal connection bar 220 may be coupled by the vertical bar 210 and the first grip 222. The first grip 222 may include a hole through which the vertical bar 210 may penetrate, and the horizontal connecting bar 220 connected to the first grip 222 may be vertically movable on the vertical bar 210. In addition, the horizontal connection bar 220 may be coupled to the first grip 222 by the fastening pin 223 to rotate on the first grip 222 about the fastening pin 223.

The light source support 250 may be connected to the horizontal connection bar 220 through the vertical connection bar 230. In addition, the light source support 250 may be coupled by the vertical connection bar 230 and the second grip 232. In this case, the light source support 250 may be coupled to rotate around the vertical connection bar 230 with a central axis.

In addition, the light detector 450 may be disposed at the lower end of the vertical connection bar 230. The light detector 450 may detect light reflected from the subject, monitor the subject, and acquire and analyze an image of the subject. The light detector 450 may use a CCD camera. The light detector 450 may be surrounded by the camera cover 440 to cover the CCD camera. The camera cover 440 may protect the light detector 450 by a physical external force, and may help to acquire a stable image. An illumination light source (not shown) may be disposed in the camera cover 400. The illumination light source is for illuminating the subject in the course of performing an examination or surgery on the subject, and a light source having white light may be used.

In addition, the filter unit 430 may be formed on a path that proceeds to the light (fluorescence) light detector 450 of a specific wavelength band from the subject. For example, in the exemplary embodiment illustrated in FIG. 1, the filter unit 430 may be disposed under the light detector 450. As shown in FIGS. 4 and 5, the filter unit 430 may be implemented in a form in which a filter wheel 434 including a plurality of filters 436 is coupled to the wheel fastening unit 432. 4 and 5 are views showing the side and the plane of the filter unit 430. Referring to this, the filter wheel 434 may be coupled to the wheel fastening unit 432 to be rotated. Therefore, the filter 436 may be selected according to the wavelength of light (fluorescence) having a specific wavelength emitted from the subject to be extracted according to the wavelength of the light source 400.

Anesthesia chamber 120 disposed on one side of the stage 100 is for anesthesia of a subject, and an anesthetic injection hole (not shown) may be formed in the anesthetic chamber 120 for inhalation anesthesia of the subject. In addition, the cover 122 of the anesthesia chamber 120 may be formed to seal the anesthesia chamber 120.

The seating stand 110 formed in the region near the anesthesia chamber 120 on the stage 100 is for seating a subject for examination and surgery, and may be formed to be detachable. Accordingly, the seating stand 110 may be separated and washed and sterilized / disinfected to prevent infection of the subject during the next experiment. In addition, a heating wire (not shown) may be formed below the seating table 110 to maintain the temperature of the subject.

And one side of the seating unit 110 may be provided with a head anesthesia 132. Head anesthesia 132 is to perform anesthesia for the subject in the process of inspecting or operating the subject disposed on the seating 110.

In addition, in order to fix the subject to the seating unit 110 may be further provided with a magnetic fixture. That is, the seating unit 110 may be formed of a panel made of metal, and the subject may be fixed to the seating unit 110 by using the magnet type fixing body 150 as shown in FIG. 4.

Referring to FIG. 6, the magnet type fixing body 150 is implemented in a form in which a fixing ring 154 is coupled to a fixing part 151 composed of a magnet part 152 and a locking rod 156.

The magnet unit 152 serves as a stand facing the seating table 110. Hanging rod 156 includes a plurality of grooves 158 for engaging the fixing ring 154. Each groove 158 may be formed at a different height on the locking rod 156 to vary the height at which the fixing ring 154 is coupled. That is, the fixing ring 154 may be coupled to the locking rod 156 at different heights depending on the subject.

7 is a view showing a state in which the subject 10 is fixed to the seating unit 110 by using the above-described magnet type fixing body 150. Since the magnetic fixture 150 is not fixed to the seating plate 110 at a specific position, it may be fluidly moved according to the size of the subject 10. In addition, since the magnetic fixture 150 can be moved even when the subject 10 is fixed by using the fixing ring 154, the movement of the subject 10 and the internal observation even in the dissected state of the subject 10. Can make it easier.

The stage 100 may include a temperature controller 146 for controlling the temperature and driving of the heating wire formed under the seating unit 110. In addition, the stage 100 may further include a light source controller 142. The light source controller 142 may control the amount of light and the operation of the LED light source.

The stage 100 may be supported by the vertical column 200. The vertical column 200 may be vertically moved up / down by the height adjusting unit 144 formed on one side of the stage 100 to adjust the height of the stage.

The vertical column may be coupled to and supported by the lower base 300. The lower base 300 supports the entire device, and includes a moving wheel and a fixing device (not shown) at the rear thereof to move the test and operating table and to fix it at a specific place.

The small animal test and operating table according to the present invention includes a device controller 500 for displaying information about an image and analyzing and storing the image. The device controller 500 may include a display unit 510, a setting unit 530 for adjusting a captured image, and an input unit 520 for manipulating the setting unit 530.

The setting unit 530 may adjust the resolution, brightness, and shutter speed and gain coefficient of the captured image, and capture and record the captured image displayed on the display unit 510. The setting unit 530 may be implemented by programmed software.

The display unit 510 may display the captured image and display the operation state of the setting unit 530 through the setting unit 530 and the input unit 520. The display unit 510 will be described with reference to FIG. 8 as follows.

First, the display unit 510 includes an image display unit 512 for displaying a captured image. In addition, setting unit interfaces 514, 516, and 518 for displaying the operation state and contents of the setting unit 530 may be included. In addition, the light source may include a light source display unit 519 that displays the type and brightness of the light source 400.

The device control unit 500 may optimize the conditions in the image observation and monitoring process for the subject to perform the operation of capturing and recording the image in real time, and increase the efficiency of the small animal inspection process.

100: stage 120: anesthesia chamber
110: seating table 400: light source
450: light detector 200: vertical column
300: lower base 150: magnet type fixed body

Claims (8)

A stage including an anesthesia chamber on one side and a seating plate made of metal for seating the subject;
A vertical column supporting the stage;
A lower base disposed below the vertical pillar to support the vertical pillar and the stage;
A light source for irradiating light to the subject to acquire an image of the subject;
A light detector for extracting light of a specific wavelength emitted from the subject to obtain an image; And
And a device control unit for storing and analyzing the image of the subject.
The method of claim 1,
The device control unit
A setting unit for adjusting a captured image;
An input unit for manipulating the setting unit; And
And a display unit for displaying a photographed image and displaying contents of the setting unit and the input unit.
The method of claim 2,
And the setting unit adjusts the resolution, brightness, and shutter speed and gain coefficient of the captured image, and captures and records the captured image displayed on the display unit.
The method of claim 1,
The seating table
A magnet part; And
And a magnet-type fixture including a connecting pin extending from the magnet part to hook a portion of the subject.
The method of claim 1,
The light source is a small animal inspection device, characterized in that consisting of a plurality of LEDs each having a unique wavelength band.
The method of claim 1,
The seating table is a small animal inspection device further comprises a head anesthesia.
The method of claim 1,
And a filter unit disposed between the test object and the light detection unit and configured to detect light having a specific wavelength emitted from the test object.
The method of claim 5, wherein
And a condenser lens disposed at a position at which light is emitted from the light source, for concentrating the light of a specific wavelength band of the light source onto the subject.

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