KR101115726B1 - Chlorophyll extraction method from mulberry leaves - Google Patents

Abstract

In the present invention, the collected mulberry leaves are pulverized and stirred with lactic acid bacteria and then aged, dried in the shade, and then ultrasonically generated in a mixed state of the solvent to extract chlorophyll from the pulverized mulberry leaves, thus reducing the manufacturing cost. The present invention relates to a method for extracting chlorophyll from mulberry leaves, which can extract a large amount of chlorophyll in an unbroken state because high temperature does not occur when ultrasonic waves are generated in the pulverized mulberry leaves.

Description

Chlorophyll Extraction Method from Mulberry Leaves {CHLOROPHYLL EXTRACTION METHOD FROM MULBERRY LEAVES}

In the present invention, since chlorophyll is extracted from pulverized mulberry leaves by generating ultrasonic waves in the pulverized mulberry leaves, the manufacturing process is simple and the manufacturing cost is reduced, and high temperatures are not generated when ultrasonic waves are generated in the pulverized mulberry leaves. The present invention relates to a method for extracting chlorophyll from mulberry leaves capable of mass extraction of chlorophyll.

In general, chlorophyll is a beneficial substance to the human body, such as to prevent cancer, to clear the blood to hematopoiesis, sterilization, microvascular expansion and promotion of metabolism, antiallergic action.

Chlorophyll can heal wounds of the intestinal tract, and has granulation regeneration to heal wounded cells and bring them back to their original state.

Therefore, ingesting a lot of chlorophyll heals wounds in the stomach and strengthens the intestines, which can give a good effect on the body's immune system as a whole, helping to prevent and treat cancer.

Chlorophyll, on the other hand, is a pigment that coexists with carotenoids in the chloroplast, which is rich in nutrition, and serves to determine the freshness of green leaf plants.

It is relatively stable and preserved when there is vitality of the plant, but when the vitality is lost, chlorophyll is browned and discolored to pheophytin due to carotene components and various organic acid enzymes contained in chloroplasts.

Therefore, it is also important to extract chlorophyll from plants, but it is also important how to keep chlorophyll from discoloring for a long time and maintaining vitality.

Despite the beneficial effects of chlorophyll on the human body as described above, there is no method of extracting pure chlorophyll, so far it is limited to the method of directly ingesting foods such as raw food, green tea, kale or wormwood.

Recently, a method of extracting chlorophyll (Korean Patent No. 10-0451566) has been proposed, but suggests that it takes 10 to 20 days in the shade in the drying step of the green leaf plants.

However, there is a problem of oxidizing and discoloring the carotene component and air in the drying step to destroy a substantial portion of chlorophyll.

In order to solve such a problem, a method of extracting chlorophyll in high yield and a method of extracting chlorophyll (Korea Patent Publication No. 10-2009-0056442) which can maintain long-term vitality without extracting chlorophyll have been proposed.

However, in the chlorophyll extraction method, chlorophyll-containing plants are collected, washed, and then immersed in brine and aged to obtain starches containing chlorophyll. There is a problem that cannot be mass extracted.

The present invention was created in order to solve the above-mentioned problems, and since the chlorophyll is extracted from the pulverized mulberry leaves by generating ultrasonic waves in the pulverized mulberry leaves, the manufacturing process is reduced and the ultrasonic waves are generated in the pulverized mulberry leaves. It is an object of the present invention to provide a method for extracting chlorophyll from mulberry leaves that can extract a large amount of chlorophyll in the unbroken state since high temperature does not occur.

The present invention for achieving the above object comprises the steps of a) harvesting and grinding mulberry leaves; b) stirring the mulberry leaves and lactic acid bacteria pulverized through the step a); c) aging the mulberry leaves stirred with the lactic acid bacteria through step b); d) drying the mulberry leaves aged through the step c) in the shade; e) extracting chlorophyll by generating an ultrasonic wave after mixing the solvent to the mulberry leaves dried in the shade through the step d); f) chlorophyll extracted through the step e) is placed in the filter cloth and dehydrated and concentrated; provides a method for extracting chlorophyll from mulberry leaves, characterized in that comprises a.

Here, the lactic acid bacteria of step b) is preferably at least one selected from the group of the genus Lactobacillus, Streptococcus, leukonostock, Pediococcus, Lactococcus and Bifidobacterium.

And, in step b), it is preferable to stir the mulberry leaf and lactic acid bacteria pulverized through the step a) at a speed of 15-20 rpm for 3-4 hours at 36.5 ℃.

In addition, the step c) is preferably the mulberry leaves stirred with lactic acid bacteria through the step b) is aged at 36.5 ℃ for 12 to 24 hours.

In addition, the solvent of step e) is preferably 94% ethanol.

Furthermore, the step e) generates ultrasonic waves using the room temperature ultrasonic extraction device 10, the room temperature ultrasonic extraction device 10 is provided between the two extraction tanks 110 and the two extraction tanks (110). And an ultrasonic wave generation unit 130 for generating ultrasonic waves into the two extraction tanks 110, and the ultrasonic wave generation unit 130 is provided with a space for receiving the cooling water therein and the cooling water is introduced and discharged. A body 132 on which an inlet 132a and an outlet 132b are formed, respectively; And a vibrator 134 provided in the space of the body 132 to generate ultrasonic waves, and transmitted between the two extraction tanks 110 and the body 132 of the ultrasonic generator 130. Preferably, the tube 150 is provided.

Hereinafter, the chlorophyll extraction method in the mulberry leaf of the present invention will be described in detail.

Chlorophyll extraction method in the mulberry leaf of the present invention is largely, a) taking the mulberry leaf and grinding; b) stirring the mulberry leaves and lactic acid bacteria pulverized through the step a); c) aging the mulberry leaves stirred with the lactic acid bacteria through step b); d) drying the mulberry leaves aged through the step c) in the shade; e) extracting chlorophyll by generating an ultrasonic wave after mixing the solvent to the mulberry leaves dried in the shade through the step d); f) putting the chlorophyll extracted through step e) into a filter cloth and dehydrating and concentrating.

First, the step a) is a step of crushing the mulberry leaves collected in May-June with high yield of chlorophyll in water and then using a grinder or the like.

Next, step b) is a step of stirring the mulberry leaves and lactic acid bacteria pulverized through the step a).

Next, step c) is a step of ripening the mulberry leaves stirred with the lactic acid bacteria through the step b).

Next, step d) is a step of drying the mulberry leaves aged in the c) step in the shade.

Next, step e) is a step for extracting chlorophyll by generating an ultrasonic wave after mixing the solvent to the mulberry leaves in a dry state in the shade through the step d).

At this time, the mulberry leaf and the solvent dried in the shade through the step d) is put into the ultrasonic extraction apparatus at room temperature to be described later to generate an ultrasonic wave.

This allows chlorophyll to be extracted from the mulberry leaves dried in the shade through the step d).

Next, the step f) is a step of separating the solvent and chlorophyll while concentrating using a vacuum concentrator and the like after dehydrating the chlorophyll extracted through the step e), for example, in a filter cloth.

At this time, the solvent can be reused, the chlorophyll concentrate is used for skin diseases such as atopic dermatitis.

On the other hand, the chlorophyll concentrate can be used in skin jars such as eczema and atopic dermatitis in a spray bottle, etc., of course, can be used as a tablet tablets.

Here, the lactic acid bacteria of step b) may be at least one selected from the genus Lactobacillus, Streptococcus, Leukonostock, Pediococcus, Lactococcus and Bifidobacterium.

As described above, the lactic acid bacteria of step b) are more than one selected from the group of the genus Lactobacillus, Streptococcus, Leukonostock, Pediococcus, Lactococcus and Bifidobacterium. By decomposing easily, there is an advantage that the mass of chlorophyll in a state that is not destroyed from the ground mulberry leaves more smoothly.

Here, in step b), the mulberry leaf and lactic acid bacteria pulverized through the step a) may be stirred at a speed of 15 to 20 rpm for 3 to 4 hours at 36.5 ° C.

At this time, the mulberry leaf pulverized through the step a) is decomposed by lactic acid bacteria, the mulberry leaf and lactic acid bacteria pulverized through the step a) when stirred at a speed of less than 15rpm for less than 3 hours at less than 36.5 ℃ 3 minutes There is a problem that the decomposition is not made smoothly.

When the mulberry leaves and lactic acid bacteria pulverized through the step a) are stirred at a speed of more than 20 rpm for more than 4 hours at 36.5 ° C., there is a problem that chlorophyll contained in the pulverized mulberry leaves may be destroyed.

As described above, the step b), the mulberry leaf and lactic acid bacteria pulverized through the step a) is stirred at a speed of 15 to 20 rpm for 3 to 4 hours at 36.5 ° C, so that the pulverized mulberry leaf is more smoothly decomposed, the grinding Chlorophyll in an unbroken state from the mulberry leaves has the advantage of more smooth mass extraction.

Here, the step c) is preferably mulberry leaves stirred with lactic acid bacteria through the step b) for 3 to 12 hours at 36.5 ℃.

When the mulberry leaves stirred with lactic acid bacteria through the step b) is aged less than 12 hours at less than 36.5 ℃ there is a problem that the ripening of the mulberry leaves stirred with lactic acid bacteria is not made smoothly.

When the mulberry leaves stirred with lactic acid bacteria through step b) is aged more than 36.5 ° C. for more than 24 hours, there is a problem in that chlorophyll in the unbroken state cannot be smoothly extracted from the ground mulberry leaves.

As described above, the step c) ripens the mulberry leaves stirred with the lactic acid bacteria through step b) at 36.5 ° C. for 12 to 24 hours, thereby making the ripening of the mulberry leaves stirred with the lactic acid bacteria more smoothly. Chlorophyll in the unbroken state from the pulverized mulberry leaves has the advantage of more smooth mass extraction.

Here, the solvent of step e) is preferably 94% ethanol.

Thus, since the solvent of step e) is 94% ethanol, the chlorophyll is more smoothly from the dried mulberry leaves when ultrasonic waves are generated in the state of mixing the solvent with the mulberry leaves dried in the shade through step d). There is an advantage that can be extracted.

1 is a cross-sectional view schematically showing a room temperature chlorophyll extracting apparatus used in the method for extracting chlorophyll from mulberry leaves of the present invention.

Here, step e) may generate ultrasonic waves using the room temperature ultrasonic extraction device 10 as shown in FIG.

The ultrasonic extraction apparatus 10 includes an ultrasonic generator 130 which is provided between two extraction tanks 110 and the two extraction tanks 110 to generate ultrasonic waves into the two extraction tanks 110. Can be done.

The ultrasonic generator 130 includes a body 132 is provided with a space for receiving the coolant therein, the inlet 132a and the outlet 132b for the inlet and outlet of the coolant, respectively; And a vibrator 134 provided in the space of the body 132 to generate ultrasonic waves.

A permeation tube 150 may be provided between the two extraction tanks 110 and the body 132 of the ultrasonic generator 130.

More specifically, after receiving the mulberry leaf and the solvent of the step e) in the dried state in the shade through the step d) in the two extraction tank 110 of the room temperature chlorophyll extractor 10 and the vibrator 134 ) To cause the vibrator 134 to generate ultrasonic waves.

On the other hand, the two extraction tanks 110 of the room temperature chlorophyll extractor 10 is not necessarily to be accommodated in the dried mulberry leaves in the shade, chlorophyll to extract all the plants containing chlorophyll such as barley sprout Of course you can.

Since the heat is generated when the vibrator 134 generates the ultrasonic waves, the temperature inside the body 132 of the ultrasonic generator 130 is increased.

At this time, the coolant accommodated in the body 132 cools the temperature inside the body 132.

And, the cooling water is a circulation pipe 20 provided between the inlet 132a and the outlet 132b formed in the body 132 of the ultrasonic generator 130 and the circulation pump provided in the circulation pipe 20 ( 30).

Therefore, the cooling water cools the temperature inside the body 132 in a state where the temperature is increased by the vibrator 134, so that the temperature inside the body 132 can always be maintained at room temperature.

The ultrasonic wave generated by the vibrator 134 is as shown in FIG. 1 through the transmission tube 150 provided between the two extraction tank 110 and the body 132 of the ultrasonic generator 130. It is transmitted to the two extraction tanks 110.

This allows chlorophyll to be extracted from the mulberry leaves dried in the shade of the state accommodated in the two extraction tank 110.

In this case, since the temperature of the ultrasonic waves transmitted to the two extraction tanks 110 is low, chlorophyll may be prevented from being destroyed during the process of extracting chlorophyll from the mulberry leaves.

As described above, the step e) includes ultrasonic waves generated between the two extraction tanks and the two extraction tanks to generate ultrasonic waves using an ultrasonic generator for generating ultrasonic waves into the two extraction tanks. Since the generator generates the ultrasonic wave and simultaneously cools the heat generated when the ultrasonic wave is generated to maintain the room temperature, there is an advantage that the chlorophyll in the unbroken state can be extracted more smoothly in large quantities from the mulberry leaf.

As described above, the present invention is simple because the pulverized mulberry leaf is pulverized with lactic acid bacteria and then aged after aging, dried in the shade, and then extracted with chlorophyll from the pulverized mulberry leaf by generating ultrasonic waves in a mixed state of a solvent. The manufacturing cost is reduced, there is an advantage that can be extracted a large amount of chlorophyll in the unbroken state because the high temperature does not occur when generating ultrasonic waves in the mulberry leaves.

In the present invention, the collected mulberry leaves are pulverized and stirred with lactic acid bacteria and then aged, dried in the shade, and then ultrasonically generated in a mixed state of the solvent to extract chlorophyll from the pulverized mulberry leaves, thus reducing the manufacturing cost. When the ultrasonic wave is generated in the pulverized mulberry leaf, high temperature does not occur, so that the chlorophyll in the unbroken state can be extracted in large quantities.

And, l) because the lactic acid bacteria of step Lactobacillus genus, Streptococcus genus, Leukonostock genus, Pediococcus genus, Lactococcus genus and Bifidobacterium genus more than one of the pulverized mulberry leaves more smoothly By decomposing, there is an effect capable of more smoothly mass extraction of chlorophyll in a state that is not destroyed from the pulverized mulberry leaves.

In addition, the step b) is because the mulberry leaf and lactic acid bacteria pulverized through the step a) is stirred at a speed of 15 to 20rpm for 3 to 4 hours at 36.5 ° C to decompose the pulverized mulberry leaf more smoothly, the grinding Chlorophyll in a state that is not destroyed from the mulberry leaves is more effective to mass extract more smoothly.

In addition, since the step c) is ripened mulberry leaves stirred with lactic acid bacteria through the step b) for 12 to 24 hours at 36.5 ℃, the mulberry leaves in a stirred state with the lactic acid bacteria is more smoothly made, the grinding Chlorophyll in a state that is not destroyed from the mulberry leaves is more effective to mass extract more smoothly.

In addition, since the solvent of step e) is 94% ethanol, the chlorophyll is more smoothly extracted from the mulberry leaves in the dried state when the ultrasonic wave is generated while the solvent is mixed with the mulberry leaves dried in the shade through step d). It can work.

Further, the step e) is because the ultrasonic wave is generated by using an ultrasonic extraction apparatus which is provided between the two extraction tanks and the two extraction tanks and includes an ultrasonic generator for generating ultrasonic waves into the two extraction tanks. The generator generates an ultrasonic wave and simultaneously cools the heat generated when the ultrasonic wave is generated to maintain the room temperature, thereby effectively extracting a large amount of chlorophyll in the unbroken state from the mulberry leaf more smoothly.

1 is a cross-sectional view schematically showing a room temperature chlorophyll extracting apparatus used in the method for extracting chlorophyll from mulberry leaves of the present invention.

Hereinafter, the method for extracting chlorophyll in the mulberry leaf of the present invention will be described in more detail with reference to Examples. Of course, the scope of the present invention is not limited to the following Examples, and does not depart from the technical gist of the present invention. Various modifications can be made by those skilled in the art within the scope.

Example 1

The mulberry leaves were collected and pulverized using a pulverizer and stirred at a speed of 15 rpm for 3 hours at 36.5 ° C. using a stirrer together with Lactobacillus strain Lactobacillus.

It was then aged for 12 hours at 36.5 ° C. and then dried in the shade.

Then, chlorophyll was extracted by generating ultrasonic waves using an ultrasonic extraction apparatus at room temperature in a mixed state of mulberry leaves and 94% ethanol, and concentrated in a vacuum concentrator to obtain a chlorophyll concentrate of Example 1.

[Example 2]

The mulberry leaves were collected and pulverized using a pulverizer and stirred at a speed of 20 rpm for 4 hours at 36.5 ° C. using a stirrer with lactic acid bacteria strain Streptococcus.

It was then aged for 12 hours at 36.5 ° C. and then dried in the shade.

Then, chlorophyll was extracted by generating ultrasonic waves using an ultrasonic extraction apparatus at room temperature in a mixed state of mulberry leaves and 94% ethanol, and concentrated in a vacuum concentrator to obtain a chlorophyll concentrate of Example 2.

Example 3

The mulberry leaves were collected and pulverized using a pulverizer and stirred at a speed of 15 rpm for 3 hours at 36.5 ° C. using a stirrer together with the lactic acid bacteria strain leuconosstock.

It was then aged for 24 hours at 36.5 ° C. and then dried in the shade.

Then, chlorophyll was extracted by generating ultrasonic waves using an ultrasonic extraction apparatus at room temperature in a state of mixing mulberry leaves and 94% ethanol dried in the shade, and concentrated in a vacuum concentrator to obtain a chlorophyll concentrate of Example 3.

Example 4

The mulberry leaves were collected and pulverized using a grinder and stirred at a speed of 20 rpm for 4 hours at 36.5 ° C. using a stirrer with lactic acid bacteria strain Pediococcus.

It was then aged for 12 hours at 36.5 ° C. and then dried in the shade.

In addition, chlorophyll was extracted by extracting chlorophyll by using an ultrasonic extraction apparatus at room temperature in a state where mulberry leaves and 94% ethanol were mixed in the shade, and concentrated in a vacuum concentrator to obtain a chlorophyll concentrate of Example 4.

Example 5

The mulberry leaves were collected and pulverized using a pulverizer and stirred at a speed of 15 rpm at 36.5 ° C. for 3 hours using a stirrer with lactic acid bacteria strain Lactococcus.

It was then aged for 24 hours at 36.5 ° C. and then dried in the shade.

Then, chlorophyll was extracted by generating ultrasonic waves using an ultrasonic extraction apparatus at room temperature in a state of mixing mulberry leaves and 94% ethanol dried in the shade, and concentrated in a vacuum concentrator to obtain a chlorophyll concentrate of Example 5.

[Example 6]

The mulberry leaves were collected and pulverized using a pulverizer and stirred with a lactic acid strain Bifidobacterium genus at a speed of 20 rpm for 4 hours at 36.5 ° C.

It was then aged for 12 hours at 36.5 ° C. and then dried in the shade.

Then, chlorophyll was extracted by generating ultrasonic waves using an ultrasonic extraction apparatus at room temperature in a state where mulberry leaves and 94% ethanol were mixed in the shade, and concentrated in a vacuum concentrator to obtain a chlorophyll concentrate of Example 6.

Comparative Example 1

Unlike Example 1, chlorophyll was extracted using a centrifuge, and then concentrated by a vacuum concentrator to obtain a chlorophyll concentrate of Comparative Example 1.

The chlorophyll concentrates of Examples 1 to 6 and the chlorophyll concentrates of Comparative Example 1 thus obtained were subjected to inorganic qualitative and mass tests and elemental analysis tests.

[Mineral Qualification and Mass Test]

Inorganic qualitative and mass tests were performed through IPC analysis, and the results are shown in Table 1.

Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Comparative Example 1 Chlorophyll 3265.7mg / 100g 3876.3mg / 100g 3304.6mg / 100g 3280.5mg / 100g 3320.3mg / 100g 4012.3mg / 100g 1873.2mg / 100g Al 15.3mg / kg 16.1mg / kg 16.3mg / kg 17.2mg / kg 16.9mg / kg 19.3mg / kg 5.1mg / kg B 6.3 mg / kg 5.4mg / kg 6.5mg / kg 5.3mg / kg 8.3mg / kg 9.5mg / kg 1.6mg / kg Ba 2.9mg / kg 2.8mg / kg 2.9mg / kg 2.6mg / kg 2.4mg / kg 5.9 mg / kg 0.2mg / kg P 82.7mg / kg 82.0mg / kg 84.3 mg / kg 83.8mg / kg 84.4 mg / kg 94.0mg / kg 51.8mg / kg Si 190.3mg / kg 189.3mg / kg 192.1 mg / kg 191.2mg / kg 192.1 mg / kg 212.3mg / kg 103.4 mg / kg Ca 939.3mg / kg 942.4mg / kg 937.2 mg / kg 936.8 mg / kg 941.2mg / kg 955.4 mg / kg 631.8mg / kg Cr 4.8mg / kg 5.3mg / kg 4.6mg / kg 5.4mg / kg 4.7mg / kg 6.3 mg / kg 1.7mg / kg Cu 7.9 mg / kg 8.0mg / kg 6.8 mg / kg 7.5mg / kg 6.9mg / kg 9.2mg / kg 3.2mg / kg Fe 55.2mg / kg 57.2 mg / kg 58.0mg / kg 54.9mg / kg 53.6 mg / kg 61.2mg / kg 36.1mg / kg K 169.4 mg / kg 171.2mg / kg 167.4mg / kg 169.3 mg / kg 173.1mg / kg 183.2mg / kg 98.4mg / kg Mg 1659.2mg / kg 1678.1mg / kg 1701.2mg / kg 1671.0mg / kg 1703.2mg / kg 1719.1mg / kg 856.3 mg / kg Mn 12.3mg / kg 13.8mg / kg 11.2mg / kg 13.1 mg / kg 12.8mg / kg 15.8mg / kg 4.7mg / kg Na 104.9mg / kg 106.9 mg / kg 101.2mg / kg 107.2 mg / kg 104.5mg / kg 113.9mg / kg 71.3mg / kg Nl 2.8mg / kg 3.0mg / kg 3.1mg / kg 2.7 mg / kg 3.1mg / kg 5.0mg / kg 0.9mg / kg Zn 12.4mg / kg 12.5mg / kg 11.8mg / kg 12.5mg / kg 13.1 mg / kg 16.5mg / kg 6.7 mg / kg

As shown in Table 1, the total chlorophyll of the chlorophyll concentrates of Examples 1 to 6 were 3265.7 mg / 100 g, 3876.3 mg / 100 g, 3304.6 mg / 100 g, 3280.5 mg / 100 g, 3320.3 mg / 100 g, and 4012.3 mg / 100 g, respectively. The total chlorophyll of the chlorophyll concentrate of Comparative Example 1 was measured to be 1873.2 mg / 100 g.

The chlorophyll extract of Examples 1 to 6 was collected by mulberry leaves and pulverized using a grinder and stirred with lactic acid bacteria, then aged for 12 to 24 hours at 36.5 ℃ and dried in the shade, mixed 94% ethanol Chlorophyll was extracted by generating ultrasonic waves using a room temperature ultrasonic extraction apparatus in one state and concentrated in a vacuum concentrator, so that the chlorophyll of the chlorophyll concentrates of Examples 1 to 6 was not destroyed, so that total chlorophyll was 3265.7 mg / 100 g and 3876.3, respectively. It is thought to be highly measured as mg / 100g, 3304.6mg / 100g, 3280.5mg / 100g, 3320.3mg / 100g and 4012.3mg / 100g.

Elemental Analysis Test

Elemental analysis was performed using an elemental analyzer, the results are shown in Table 2.

element Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Comparative Example 1 C 73.21% 74.23% 72.23% 74.12% 75.32% 76.19% 51.12% H 7.87% 8.31% 7.29% 8.02% 8.0% 9.42% 3.12% O 10.92% 11.12% 10.11% 11.02% 10.87% 13.11% 5.42% N 0.85% 0.82% 1.02% 0.9% 1.01% 1.02% 0.16%

The contents of C (carbon), H (hydrogen), O (oxygen) and N (nitrogen) elements contained in the chlorophyll concentrates of Examples 1 to 6 and the chlorophyll concentrate of Comparative Example 1 are as shown in Table 2, respectively. same.

The chlorophyll extract of Examples 1 to 6 was collected by mulberry leaves and pulverized using a grinder and stirred with lactic acid bacteria, then aged for 12 to 24 hours at 36.5 ℃ and dried in the shade, mixed 94% ethanol Chlorophyll was extracted by generating ultrasonic waves using a room temperature ultrasonic extraction apparatus in one state, and then concentrated by a vacuum concentrator, so that the chlorophyll of the chlorophyll concentrates of Examples 1 to 6 was not destroyed, so that the chlorophyll concentrates of Examples 1 to 6 were The contents of C (carbon), H (hydrogen), O (oxygen) and N (nitrogen) elements contained in the chlorophyll concentrate are contained in the chlorophyll concentrate of Comparative Example 1 (C), H (hydrogen), It is considered that the content of the O (oxygen) and N (nitrogen) elements is significantly higher than that of the elements.

10; Room temperature ultrasonic extraction apparatus, 110; Extraction tank,
130; Ultrasonic generator, 132; Body Part,
132a; Inlet, 132b; outlet,
134; Oscillator, 150; Permeate Tube,
20; Circulatory tube, 30; Circulation pump

Claims (6)

a) collecting and grinding the mulberry leaf;
b) stirring the mulberry leaves and lactic acid bacteria pulverized through the step a);
c) aging the mulberry leaves stirred with the lactic acid bacteria through step b);
d) drying the mulberry leaves aged through the step c) in the shade;
e) extracting chlorophyll by generating an ultrasonic wave after mixing the solvent to the mulberry leaves dried in the shade through the step d);
f) chlorophyll extracted in step e) put in a filter cloth and dehydrating and concentrating; chlorophyll extraction method in mulberry leaves, characterized in that comprises a.
The method of claim 1,
The lactic acid bacteria of step b) is chlorophyll extract method from mulberry leaves, characterized in that at least one selected from the genus Lactobacillus, Streptococcus, Leukonostock, Pediococcus, Lactococcus and Bifidobacterium.
The method of claim 1,
The step b) is chlorophyll extraction method in the mulberry leaf, characterized in that the mulberry leaf and lactic acid bacteria pulverized through the step a) at 36.5 ℃ stirred for 3 to 4 hours at a speed of 15 ~ 20rpm.
The method of claim 1,
The c) step is chlorophyll extraction method in mulberry leaves, characterized in that the mulberry leaves stirred with lactic acid bacteria through the step b) at 36.5 ℃ for 12 to 24 hours.
The method of claim 1,
Chlorophyll extraction method in mulberry leaves, characterized in that the solvent of step e) 94% ethanol.
The method of claim 1,
In step e), ultrasonic waves are generated by using the ultrasonic extraction apparatus at room temperature,
The room temperature ultrasonic extraction device 10 includes an ultrasonic generator 130 which is provided between two extraction tanks 110 and the two extraction tanks 110 to generate ultrasonic waves into the two extraction tanks 110. Done by
The ultrasonic generator 130 includes a body 132 is provided with a space for receiving the coolant therein, the inlet 132a and the outlet 132b for the inlet and outlet of the coolant, respectively; And a vibrator 134 provided in a space of the body 132 to generate ultrasonic waves.
Chlorophyll extraction method in the mulberry leaf, characterized in that the permeation tube 150 is provided between the two extraction tank 110 and the body 132 of the ultrasonic generator 130.

Images