KR101114355B1 - Novel use of lipolytic enzyme for formation of anti-fingerprint coating, method of forming anti-fingerprint coating, substrate comprising the anti-fingerprint coating formed by the method, and product comprising the substrate - Google Patents

Abstract

The present invention provides a novel use of a lipolytic enzyme for the formation of an anti-fingerprint coating, a method of forming an anti-fingerprint coating comprising treating a substrate comprising a composition comprising a lipolytic enzyme, an anti-fingerprint formed according to the method. Provided are substrates comprising a coating and articles comprising such substrates. The anti-fingerprint coating according to the present invention can reduce contamination by fingerprints of displays, electronic products, building interior materials, and the like.

Description

Novel uses of lipolytic enzymes for the formation of anti-fingerprint coatings, methods of forming anti-fingerprint coatings, substrates comprising the anti-fingerprint coatings formed thereby, and products comprising the same {NOVEL USE OF LIPOLYTIC METHOD OF FORMING ANTI-FINGERPRINT COATING, SUBSTRATE COMPRISING THE ANTI-FINGERPRINT COATING FORMED BY THE METHOD, AND PRODUCT COMPRISING THE SUBSTRATE}

The present invention provides a novel use of a lipolytic enzyme for the formation of an anti-fingerprint coating capable of imparting a self cleaning function to a surface of a substrate, a method of forming an anti-fingerprint coating using a lipolytic enzyme, and the method. A substrate comprising an anti-fingerprint coating formed according to the present invention and a product comprising the substrate.

In the case of various display products, high-gloss electronic products or building interior materials, fingerprint contamination is one of the most common pollutions. Such contamination is clearly identified in the field of view, resulting in poor product appearance. In particular, with the recent development of touch screen interface technology of electronic devices, the fingerprint contamination on the display surface is increasing, and the necessity of solving the fingerprint contamination problem on the display surface is increasing. Nevertheless, the technology that has realized the true anti-fingerprint coating to date has not been developed, but only the anti-fouling coating of the Easy Cleaning concept has been developed.

For example, 27.6 parts by weight-36.2 parts by weight of polysilicate disclosed in Patent Document 1, 10.6 parts by weight or less of any one of epoxy and vinyl resins, 21.2 parts by weight-42.6 parts by weight of colloidal silica, 10.6 weight total of an additive consisting of at least one selected from the first group consisting of -OH, -NH ₂ and -COOH, and at least one additive selected from the second group consisting of -CnF _{2n +1} and -SiR ₃ It can illustrate about the anti-fingerprint coating for the stainless steel outer case of household appliances characterized by containing below.

In addition, the antifouling composition disclosed in Patent Document 2 may include a cured or crosslinked polymer without a perfluoropolyether moiety and a fluid fluorinated alkyl- or alkoxy-containing polymer or oligomer. .

However, such a fouling resistant film mainly uses a fluorine-based coating to wipe off the surface using a low surface energy when contaminants are transferred to the surface. It does not have the function of reducing transcription or decomposing fingerprints, and the appearance cannot be improved until the contaminants are cleaned.

In addition, the conventional anti-fingerprint coating can be mainly applied to the steel sheet used for the outer case in the application field, there is a limit to the application of the portion that requires high light transmittance, such as a display device.

Meanwhile, a coating solution, a coating film, or a coating method having a self-cleaning concept using enzymes has also been developed. However, this is mainly intended to prevent marine organic matter from attaching to the bottom of the vessel, and does not reduce contamination by fingerprints such as displays, exteriors of electronic products, interior materials for construction, and the like.

For example, the self-polishing anti-fouling coating composition disclosed by patent document 3, and the pollution prevention method of the underwater apparatus by the marine organism disclosed by patent document 4 can be illustrated.

In other words, the coating solution, coating film, or coating method of the self-cleaning concept using a conventional enzyme is used to remove or decompose adsorbent materials produced by marine organisms in advance so that marine organisms do not adhere to the bottom of a ship or the like. It has a mechanism to do this, and has nothing to do with breaking down fingerprint contamination.

As described above, there is no technology related to the fingerprint coating of the self-cleaning concept that can be applied to the use for imparting fingerprinting to the display surface to date, at least within the limits of the applicant.

WO 09072738 A1 US 20020192181 A1 US 20080038241 A1 US 5998200 B1

The present invention is to provide a technique that can be removed without hiding or wiping the fingerprint by decomposing the fingerprint components transferred using an enzyme to reduce the physical property variation between the fingerprint components.

Accordingly, it is an object of the present invention to provide a method for forming an anti-fingerprint coating having a reduced transcription and fingerprint degradation characteristics of a fingerprint, a substrate comprising the anti-fingerprint coating formed according to the method, and an article comprising the substrate.

The inventors have studied a method for forming an anti-fingerprint coating which can provide a self cleaning function instead of a stain resistant coating that simply provides an easy cleaning function. More specifically, the inventors have focused on the fact that most of the components of the fingerprint are lipids, and assuming that the coating of the lipolytic enzyme on the substrate can reduce the fingerprints transcribed by the enzyme, the lipolytic enzyme is coated on the substrate Observing changes in the physical properties of the transferred fingerprints confirmed that this coating exhibited fingerprint properties.

The components of the fingerprints are mostly sweat and sebum, and also contain contaminants such as keratin from the skin and dust from outside. Among them, sebum is the main factor that leaves traces on the appearance of products such as electronic products, and the components of sebum are triglycerides, wax monoesters, fatty acids, and squalene. , Lipids such as trace amounts of cholesterol, cholesteryl esters and the like (PW Wertz, Int. J Cosmet. Sci. 2009, 31: 21-25). Among the components of sebum, triglycerides and wax monoesters account for almost 70% of the total, and these substances are structures in which several fatty acids are bound by ester bonds. In the case of breaking the ester bond, the sebum component is mostly in the form of fatty acids, especially oleic acid, which increases homogeneity and can be converted into a lower molecular weight material. Ultimately, oleic acid can be completely released from the product by breaking down or modifying it into lower molecular weight materials, increasing volatility.

Accordingly, the present invention provides novel uses of lipolytic enzymes for the formation of anti-fingerprint coatings, ie, methods of forming anti-fingerprint coatings using lipolytic enzymes.

More specifically, the present invention provides a method of forming an anti-fingerprint coating comprising treating a substrate with a composition comprising a lipolytic enzyme.

In the present invention, the lipolytic enzyme is a fingerprint such as triglycerides, wax monoesters, fatty acids, squalene, cholesterol, cholesteryl esters, and the like. Any enzyme having a property capable of hydrolyzing the lipid component of the compound is included.

Lipase is a representative example of an enzyme having hydrolytic activity of an ester bond at room temperature. The kind or origin of the lipase is not particularly limited, and any lipase can be used as the lipolytic enzyme of the present invention. Positional nonspecific lipases may be preferred to achieve high degree of hydrolysis against triglycerides and wax monoesters, which are the main components of sebum. Currently, various lipases produced using microorganisms are commercially available from Novozymes, Amano enzyme, etc., and lipases can also be produced by transformants into which lipase structural genes are inserted.

In addition to lipases, enzymes having lipolytic activity are well known in the art. For example, a large number of proteases are known as lipolytic enzymes having lipolytic activity, and in addition, cutinase and the like are known to have lipolytic activity.

Compositions comprising lipolytic enzymes for the formation of anti-fingerprint coatings may further comprise at least one enzyme selected from the group consisting of proteases, amylases, cellulases and lactases. For example, proteases may be fixed to the surface and used together to decompose various proteins buried in the fingerprint. Proteases are used to decontaminate proteins and peptide bonds to remove contamination. In addition, enzymes such as amylase, cellulase and lactase may be further used to remove components secreted by sweat and components derived from various external contaminants.

In addition, the composition may further include a substance capable of stabilizing the enzyme in addition to the enzyme. For example, the composition may include a buffer solution such as a phosphate buffered saline (PBS) buffer solution, potassium phosphate buffer solution, sodium phosphate buffer solution, and the like. In order to preserve the activity of the enzyme, polyhydric alcohols such as polyethylene glycol and propylene glycol may be coated on the substrate together with the enzyme in the form of a polymer, or polyurethane, acrylic organic and siloxane organic or inorganic compounds may be coated on the substrate together with the enzyme. .

Compositions other than the enzymes listed above may be coated with the enzyme, but may also be sequentially coated on the substrate. That is, the composition except for the enzyme may be first coated on the substrate, and then the enzyme may be coated on the substrate by adsorption, covalent bonding, or the like.

On the other hand, lipolytic enzymes require water to hydrolyze lipids. Fingerprints contain water in addition to lipids, so it is not necessary to supply water separately.However, the hydrogel component may be included in the composition or coated on the surface of the substrate to provide more water and improve enzyme stability. Can be. In order to make a hydrogel component, it is possible to use a material in which a cross-linker containing two or more double bonds and a water-friendly functional group exist simultaneously. Such components include, for example, ethylene glycol series and acrylamide series having a multifunctional group. The hydrolysis of triglycerides and wax monoesters can be promoted more by the supply of water and the enzyme stabilization effect by the hydrogel coating.

On the other hand, the substrate on which the anti-fingerprint coating is formed is not particularly limited, and any may be applicable. For example, products that require the formation of anti-fingerprint coatings are products that have a lot of hand contact in daily life such as display products, the appearance of electronic products, and architectural interior materials. Most of these products have a surface including various plastics, glass, or the like, or have a surface coated with various varnish or protective coatings such as UV coating. In one embodiment, the substrate can be plastic or glass. For example, the substrate may include at least one polymer or glass selected from polyester, polypropylene, polyethylene terephthalate, polyethylene naphthalate, polycarbonate, triacetylcellulose, olefin copolymer, and polymethylmethacrylate. . In addition, the substrate also includes a substrate on which various coatings, such as a gloss coating, a protective coating, a coating coating, and a hydrogel coating, are formed on the surface of a substrate including a polymer or glass.

The method of treating the composition containing the lipolytic enzyme to the substrate is also not particularly limited, and any method known in the art may be used. Methods for immobilizing enzymes are well known in the art. For example, lipolytic enzymes can be introduced to the surface of the substrate by adsorption, covalent bonds or encapsulation.

Adsorption means that the lipolytic enzyme adheres to the surface of the substrate or to the coating layer of the anti-fingerprint composition except for the enzyme by physical binding force. Proteins that make up enzymes are very strong in their adsorption on the surface of an object. Thus, lipolytic enzymes can be immobilized on the surface of the substrate by adsorption without any additional treatment. The following examples show that the fixation of lipolytic enzymes by such adsorption has good stability.

In order to introduce a lipolytic enzyme to the surface of the substrate, a covalent bond is formed between the substrate and the enzyme or between the coating layer and the enzyme of the anti-fingerprint coating composition except for the enzyme, such as cyanogen bromide and acid azide derivative. Various known techniques exist, such as azide derivatives, condensing reagents, diazo coupling, alkylation, carrier cross-linking, and the like.

For example, the carrier cross linking method is a method of forming a covalent bond between a functional group present on the surface of a substrate and a functional group present on a lipolytic enzyme using a bifunctional cross-linker. Since lipolytic enzymes have various functional groups in addition to amino groups and carboxyl groups, if a functional group capable of covalently bonding with these functional groups is present on the surface of the substrate, covalent bonds can be easily formed using a bifunctional cross-linker. In this case, the functional group present on the surface of the substrate may be a functional group originally contained in the substrate or introduced into the surface of the substrate to form a covalent bond with the enzyme, or may be a functional group included in the anti-fingerprint composition except for the enzyme. . For example, when the substrate is a plastic material, the functional group present on the surface of the substrate may be directly used, and the desired functional group may be introduced to the surface through plasma treatment, primer treatment, or the like. In addition, when the substrate is glass, the functional group may be introduced into the surface through SAM (self-assembled monolayer) treatment using a siloxane-based organic compound, but is not limited thereto. Functional groups for forming covalent bonds with enzymes include amino groups, amide groups, carboxyl groups, aldehyde groups, hydroxy groups, hydroxyl groups, thiol groups, and the like. The functional groups present on or introduced to the surface may vary with the type of substrate.

In one embodiment, the covalent bond is selected from the group consisting of a) an amine, an amide, a carboxyl, an aldehyde, a hydroxyl and a thiol on the surface Treating the substrate having at least one functional group with a solution comprising a bifunctional cross-linker; b) can be formed through a process comprising immersing the substrate in a buffer containing a lipolytic enzyme.

Bifunctional cross-linkers used for induction of covalent bonds include bis-imidoesters, bis-succinimidyl derivatives, and bifunctional aryls. Bifunctional aryl halides, bifunctional acrylating agents, dialdehydes, diketones, etc. may be mentioned, but are not limited thereto. One embodiment of the present invention shows an example in which covalent bonds are induced by using glutaraldehyde which is dialdehyde.

In another embodiment, the covalent bond can be formed through a process comprising immersing a substrate having an epoxy group on its surface in a buffer solution containing a lipolytic enzyme. In addition, the fixation of the enzyme can be further improved by applying a heat treatment or UV treatment at a level that does not impair the activity of the enzyme to the substrate subjected to the above process.

As described above, the method of immobilizing the enzyme using covalent bonds was described in more detail in the following examples.

In addition, encapsulation means a method of immobilizing an enzyme by trapping a lipolytic enzyme between these substances with another substance. In one embodiment, the encapsulation may be carried out by coating a gel matrix, microcapsule, hollow fiber or membrane on the surface of the substrate and introducing a lipolytic enzyme. have. For example, a membrane made of cellulose such as cellulose nitrate or cellulose acetate, polycarbonate, nylon, nylon, and fluororesin such as PTFE (polyfluorotraethylene) may be used.

The process of coating a gel matrix, a microcapsule, a hollow fiber or a membrane, and introducing a lipolytic enzyme may be performed simultaneously or sequentially. That is, a gel matrix, a microcapsule, a hollow fiber or a membrane is first coated on the surface of the substrate, and then the substrate is immersed in a buffer solution containing a lipolytic enzyme, or It is possible to introduce lipolytic enzymes simultaneously with coating a gel matrix, microcapsule, hollow fiber or membrane on the surface. For example, in an encapsulation method using a gel matrix, a mixed solution is prepared by adding an enzyme when preparing a sol solution in a sol-gel reaction step as well as a method of adsorbing an enzyme after coating and curing the gel matrix. It is also possible to use a method of making and curing it by coating it on a substrate.

The encapsulation method using the gel matrix of the above method is more advantageous for preserving and promoting the activity of the enzyme. Any kind of gel can be applied, which guarantees mechanical strength and optical properties. For example, a double network by strengthening the mechanical strength in a coating layer or polyethylene glycol (PEG) prepared by the sol-gel method using tetramethoxysilane (TMOS), tetraethoxysilane (TEOS), glycidoxypropyl trimethoxysilane (GPTMS), etc. After the primary coating using a hydrogel (double gel) and the like to form a (double network) it can be encapsulated (encapsulation) to the enzyme. A more detailed description is given in the following examples.

On the other hand, the buffer solution containing a lipolytic enzyme used in the above methods include PBS (phosphate buffered saline) buffer solution, potassium phosphate buffer solution, sodium phosphate buffer solution, etc. It is not limited only to this. Lipolytic enzymes contained in the buffer solution in principle to fix the amount to cover the surface of the substrate to be fixed with a mono layer (mono layer). Most commercial lipolytic enzymes are in the form of small amounts of enzymes in the excess of an extender (extender) such as dextrin, lactose, stabilizer, etc., and the amount of enzyme is determined by considering only the content of protein alone. In the case of covalent bonds, the amount of enzyme is determined by calculating the amount of protein corresponding to the functional group on the surface of the substrate, and in the case of adsorption and encapsulation, the amount corresponding to 3 to 10 times the amount of protein that can cover the surface of the substrate is determined. It is preferable to use dissolved in buffer solution.

The present invention also provides a substrate comprising an anti-fingerprint coating formed according to the method described above. As can be seen through the following examples, substrates comprising anti-fingerprint coatings immobilized with a lipolytic enzyme according to the above method exhibit fingerprint properties through fingerprint degradation and reduced fingerprint transcription. Such anti-fingerprint coatings may be laminated in single or multiple layers on the surface of the substrate. The anti-fingerprint coating may be formed on the surface of the substrate to a thickness of 20 nm to 200 μm. The thickness of the coating may reach 200 μm depending on the type and content of the coating composition at the 20 nm level when the single layer coating. However, the thickness of the coating should be adjusted at a level that does not interfere with the optical properties required for the substrate. If the thickness of the anti-fingerprint coating layer is less than 20nm may cause a problem that the decomposition of the fingerprint component is limited, if it exceeds 200㎛ may cause a problem that the optical transmittance is lowered.

In order to maximize the spreading effect of the fingerprint component, the surface energy of the anti-fingerprint coating layer is preferably 20 to 50 mN / m. When the surface energy of the anti-fingerprint coating layer is less than 20 mN / m, the fingerprint component may not spread, and when the fingerprint energy exceeds 50 mN / m, it may not be easy to remove the fingerprint. Here, when the lipase is coated with an example of the enzyme, the surface energy measurement result is 30 to 50 mN / m, and in this range, the spreading effect of the fingerprint may be maximized, and as a result, fingerprint transcription may be reduced.

The present invention provides an article comprising a substrate comprising the anti-fingerprint coating. Products comprising a substrate comprising an anti-fingerprint coating according to the present invention may be products with a lot of hand contact in daily life, the kind is not particularly limited. For example, such products include display devices, electronic devices, building interior materials, and the like. For example, the display device may be selected from the group consisting of a liquid crystal display (LCD), an organic light emitting display (OLED), and a plasma display panel (PDP). Currently, portable display apparatuses that have a touch screen type interface can greatly improve the aesthetics of a product when the anti-fingerprint coating according to the present invention is introduced.

The method of introducing an anti-fingerprint coating to the product is also not particularly limited. That is, the lipolytic enzyme may be directly coated on the surface of the substrate of the product such as the display device, or the substrate in the form of a film coated with the lipolytic enzyme may be attached to the surface of the product.

Anti-fingerprint coating according to the present invention can reduce the contamination by fingerprints, such as the display device, the appearance of electronic products, building interior materials.

1 is a graph showing a result of measuring haze value after transferring a fingerprint to a substrate having an anti-fingerprint coating formed according to Example 1. FIG.
2 and 3 are NMR spectra of the results of degradation after transfer of a major fingerprint component to a substrate having an anti-fingerprint coating formed according to Examples 1 and 2. FIG.
4 and 5 are NMR spectra of results of degradation after transfer of a major fingerprint component to a substrate having an anti-fingerprint coating formed according to Example 3. FIG.
6 and 7 are graphs measured by hazemeters for the results of disappearance after actual fingerprint transfer to a substrate having an anti-fingerprint coating formed in accordance with Example 3. FIG.
FIG. 8 is a graph of a result obtained by performing a wiping test on a substrate having an anti-fingerprint coating formed according to Example 3 and then disappearing after transferring the actual fingerprint, using a hazemeter.
9 and 10 are graphs obtained by measuring hazemeter results of disappearance of contaminants when complex contaminants are applied to a substrate having an anti-fingerprint coating formed according to Example 3. FIG.

Advantages and features of the present invention and methods for achieving them will be apparent with reference to the embodiments described below in detail. However, the present invention is not limited to the embodiments disclosed below, but will be implemented in various forms, and only the embodiments are intended to complete the disclosure of the present invention, and the general knowledge in the technical field to which the present invention pertains. It is provided to fully convey the scope of the invention to those skilled in the art, and the present invention is defined only by the scope of the claims.

[ Example ]

< Example  1> using covalent bonds Lipolytic  Fixation of enzymes

Lipase was coated on the glass substrate by the following method.

The slide glass surface-coated with amino alkyl silane was reacted with a 10% solution of glutaraldehyde for 2 hours. Next, the slide glass was lightly washed with distilled water, and then immersed in PBS buffer containing lipase (Amano Enzyme, Lipase PS “Amano” SD, Burkholderia cepacia) at a concentration of 100 mg / mL and allowed to stand at room temperature for 24 hours. Then, the lipase-fixed slide glass was sufficiently washed with flowing distilled water, and then again put in distilled water and gently shaken for 40 minutes. Completed.

< Experimental Example  1> Anti-fingerprint  Fingerprint by coating Warrior  Confirm reduction

After preparing three specimens with fingerprints transferred to the lipase-fixed slide glass as in Example 1, the haze value over time was measured and the results are shown in FIG. 1. Here, the comparison group (control glass (w / o lipase)) was compared as a slide glass treated only with amino alkyl silane and glutaraldehyde.

Looking at the haze value measured immediately after the fingerprint was taken (0hr), compared to the comparative group showed a lower value on the lipase-coated substrate, it can be seen that less fingerprints on the lipase-fixed substrate. In addition, as time increases, it was confirmed that the haze value of the fingerprint was degraded in the lipase-coated substrate.

As described above, the fingerprint transfer was performed on the surface to which the lipase was immobilized, and the microscopic observation and the haze (Haze) measurement experiment showed that the surface to which the lipase was immobilized had a lower fingerprint transfer degree than the surface to which the lipase was not fixed.

< Example  2> using epoxy group Lipolytic  Fixation of enzymes

In addition to the method described in Example 1 as a method of coating the lipase using a chemical covalent bond was also fixed by using an epoxy (epoxy) group. The epoxy glass-treated slide glass (Superchip Glass ES, Slide epoxy silane, nunc ^TM ) was immersed in sodium phosphate buffer containing lipase at a concentration of 100 mg / mL, and allowed to stand at room temperature for 4 hours, followed by 50 to 55 ° C. The reaction was carried out in an oven for 30 minutes. The slide glass was taken out, immersed in distilled water for 15-20 times, rinsed and soaked in sufficient distilled water for 20 minutes and washed three times. Blowing with compressed nitrogen and drying at room temperature.

< Example  3> Fixation of Hydrolase by Adsorption on Gel Matrix

The gel matrix was used to adsorb the hydrolase and coat the surface of the substrate. In this method, the gel matrix stabilizes the enzyme, increases the efficiency of the enzyme, and has the advantage of performing a functional coating together through various gel matrices. Enzymes may be lipase alone or a combination of lipase and amylase, lipase and protease. First, a gel matrix was coated on a slide glass using a siloxane composition. This was carried out by the method of Example 1 of Korean Laid-Open Patent No. 1998-0002185. The gel matrix slide thus prepared was immersed in PBS buffer containing the enzyme at a concentration of 100 mg / mL and allowed to stand at room temperature for 24 hours. The slides were taken out, washed in the same manner as in Example 2, and then blown with compressed nitrogen and dried at room temperature.

< Experimental Example  2> Anti-fingerprint  Confirmation of Lipid Degradation Effect by Coating

In order to determine whether the glass has a fingerprint decomposition performance in the glass prepared by coating the lipase by the chemical covalent bonding method as in Examples 1 and 2, the experiment was performed using triglyceride which is the main component of the fingerprint. Triglyceride was used as triolein, which was applied to the glass surface and left at room temperature for 24 hours to analyze whether triolein was decomposed by 1 H-NMR. As a result, as shown in FIGS. 2 and 3, it was confirmed that an acid peak, which does not appear in the reference (triolein), appeared in the lipase coating slide.

< Experimental Example  3> Anti-fingerprint  Confirmation of Lipid Degradation Effect by Coating

As described in Example 3, the experiment was performed using triglyceride, which is the main component of the fingerprint, in order to check whether the glass has a fingerprint decomposition performance in a glass prepared by coating a lipase with a gel matrix. Triglycerides were used for triolein, which was applied to the glass surface and left at room temperature for 24 hours to analyze whether triolein was decomposed by 1 H-NMR. As a result, as shown in FIGS. 4 and 5, it was confirmed that an acid peak, which does not appear in the reference (triolein), appeared in the lipase coating slide.

< Experimental Example  4> Anti-fingerprint  Confirmation of fingerprint decomposition performance by coating

Haze the degree of disappearance after transfer of the actual fingerprint using the palm in order to determine whether fingerprints in the glass prepared by coating the lipase with the gel matrix (gel matrix) as in the method of Example 3 The value was confirmed through measurement. Haze change for 48 to 96 hours was observed for each hour under various conditions of temperature and humidity using a constant temperature and humidity tester. The results are shown in FIGS. 6 and 7. The degree of change of ΔH over time was represented by setting the haze value (ΔH) increased due to fingerprint transfer to 100%. Significant decrease in haze in the lipase-added specimens (labeled 'w / lipase') when compared to specimens prepared by the method of Example 3 but not added to the lipase (denoted 'w / o lipase') I could confirm it.

< Experimental Example  5> Anti-fingerprint  Confirmation of fingerprint decomposition performance by coating

Wiping test was performed to confirm that the fixation of the lipolytic enzyme by adsorption in the glass prepared by coating the lipase together with the gel matrix as in the method of Example 3 has excellent stability.

The glass specimen was placed, and a wiping specimen was prepared by reciprocating 100 times with zero vacuum while applying a weight of 1 kg. The degree of disappearance after the actual fingerprint was transferred to the specimen and the specimen that was not wiped with no vacuum was confirmed by measuring the haze value. The change in haze for 48 hours was observed at 50 ° C. and 30% relative humidity by time using a constant temperature and humidity tester. The results are shown in FIG. 8. The degree of change of ΔH over time was represented by setting the haze value (ΔH) increased due to fingerprint transfer to 100%. Although it was prepared by the method of Example 3 compared to the non-wiping specimens (denoted no wiped) was confirmed that the performance was not reduced in the wiped specimens (denoted 100 times wiping).

< Experimental Example  6> Anti-fingerprint  Confirmation of fingerprint decomposition performance by coating

How to coat the enzyme with a gel matrix (gel matrix) as in the method of Example 3, or to determine whether the decomposition performance of the various contaminants in the glass prepared by adding other hydrolytic enzymes in addition to lipase is better In order to perform the experiment. Three kinds of specimens were prepared: a specimen prepared by adding lipase alone, a specimen prepared by adding lipase and amylase together, and a specimen prepared by adding lipase and protease together. The value was confirmed through measurement. The results of observing the change in haze with time after transferring the pollutant made by mixing oil and starch are shown in FIG. 9, and the result of observing the change in haze with time after transferring the pollutant made by mixing oil and egg white. 10 is shown. Through two experiments, it was confirmed that the removal performance of complex contaminants is improved when other hydrolase is added together than when lipase alone is added.

From the above results, it can be seen that the present invention can realize the fingerprint performance by coating the enzyme on the surface in a relatively simple method, it can be applied to most substrates that require fingerprint properties.

Claims (13)

A method of forming an anti-fingerprint coating comprising treating a composition comprising a lipolytic enzyme to a substrate selected from plastic or glass.
The method according to claim 1,
And said lipolytic enzyme is a lipase.
The method according to claim 2,
Wherein said composition further comprises one or more enzymes selected from the group consisting of proteases, amylases, cellulases and lactases.
delete The method according to claim 1,
Wherein said plastic comprises at least one polymer selected from polyester, polypropylene, polyethylene terephthalate, polyethylene naphthalate, polycarbonate, triacetylcellulose, olefin copolymers and polymethylmethacrylate. .
The method according to claim 1,
Wherein said lipolytic enzyme is introduced to the surface of the substrate by adsorption, covalent bonding or encapsulation.
The method of claim 6,
The covalent bond is a) at least one functional group selected from the group consisting of an amino group (amine), amide group (amide), carboxyl group (carboxyl), aldehyde group (aldehyde), hydroxy group (hydroxyl) and thiol group (thiol) on the surface Treating a substrate having a solution comprising a bifunctional cross-linker; b) A method of forming an anti-fingerprint coating formed by a process comprising immersing the substrate in a buffer containing a lipolytic enzyme.
The method of claim 6,
The covalent bond is a method of forming an anti-fingerprint coating is formed through a process comprising immersing a substrate having an epoxy group on the surface in a buffer solution containing an enzyme.
The method of claim 6,
The encapsulation is performed by coating a gel matrix, a microcapsule, a hollow fiber or a membrane on the surface of the substrate, and introducing a lipolytic enzyme. Forming method.
delete delete delete delete

Images