KR101096926B1 - Method for regulation of biological activity, and various apparatuses utilizing the method - Google Patents

Abstract

SUMMARY OF THE INVENTION An object of the present invention is to provide a method for controlling the activity of microorganisms, an ice making device having an automatic antibacterial treatment function and a washing machine having an automatic antibacterial treatment function. The microorganism activity control method has a peak wavelength in the range of 300 to 600 nm and irradiates the microorganism and / or animal plant cells with light having an irradiation intensity of 500 to 500,000 mW / cm 2, while the ion, preferably silver It is assumed that the ions are brought into contact. An ice making device with an automatic antibacterial treatment function and a washing machine with an automatic antibacterial treatment function have a structure capable of executing this microbial activity control method.

Metal plate, metal ion container, bacteria solution, light irradiation part, test solution

Description

METHOD FOR REGULATION OF BIOLOGICAL ACTIVITY, AND VARIOUS APPARATUSES UTILIZING THE METHOD}

This invention consists of the 1st-4th invention group which a basic principle is common.

The first invention group of the present invention relates to an antimicrobial technique that uses ions to prevent the growth of microorganisms (particularly fungi).

The second invention group of the present invention relates to a method of controlling the proliferative activity of cells using ions, and more particularly, to a method of controlling the proliferative activity of animal cells and a cell activity control device to which the method is applied.

The 3rd invention group of this invention relates to the refrigerator provided with the ice making apparatus provided with the antibacterial treatment function which applied the antibacterial principle of the 1st invention group.

The 4th invention group of this invention relates to the rotating drum type washing machine provided with the antibacterial treatment function which applied the antibacterial principle of the 1st invention group.

Hereinafter, the content is described in order by dividing each invention group.

[First invention group]

In recent years, with increasing demand for health, industrial fields such as household appliances, kitchen appliances, cosmetics, stationery, furniture and ornaments, slime control agent for paper and pulp, and wood preservatives, etc. In various fields such as medical fields, such as curtains, building materials, and medical instruments, the demand for removing microorganisms (prokaryotes, eukaryotes, etc.) is increasing.

Antimicrobial agents used to remove microorganisms include organic compounds (synthetic compounds, natural compounds, etc.) and inorganic compounds (metals such as silver, copper, zinc, and photocatalysts such as titanium oxide), but organic compounds conventionally developed as pesticides and pharmaceuticals. This has been used mainly. The organic compound used conventionally has a broad antimicrobial spectrum, and has the outstanding immediate effect and bactericidal property.

However, these organic compounds have a large load on the human body and the environment, and are likely to cause problems in safety. For this reason, in recent years, attention has been paid to metals having antimicrobial properties such as silver, copper and zinc, compounds containing these metals, and inorganic antibacterial agents which are antibacterial by photocatalytic action such as titanium oxide.

Focusing on the type of metal of the inorganic antimicrobial agent, silver ions and mercury ions are particularly excellent in inhibiting bacterial growth, followed by zinc ions, copper ions, and cadmium ions. Mercury and cadmium have a great adverse effect on the environment, and therefore are not suitable for wide use as an antibacterial material. However, silver ions, copper ions, and zinc ions have a smaller adverse effect on the environment and the like than the former. Silver ions are particularly excellent as antibacterial agents because their antibacterial activity is 200 times that of copper and 1000 times that of zinc, and the adverse effects on the environment can be reduced.

Therefore, although the use of the antimicrobial agent which used silver is expanded in recent years, even if the antimicrobial agent which uses silver is used for a wide range of uses, the chance of spillage into the natural environment increases, and also a problem such as environmental pollution arises. In particular, since silver has a strong antibacterial effect, even if a small amount of silver ions are spilled into the environment, there is a fear that it will have a significant effect on the ecosystem.

As a result, restrictions on the concentration of silver ions in drinking water are also increasing. For example, in the United States, the concentration of silver ions in drinking water is regulated to less than 100 ppb, which is becoming more stringent.

Therefore, it is necessary to reduce the amount of metals contained in the inorganic antimicrobial agent and to minimize the outflow of useless metal ions from the viewpoint of environmental conservation, the viewpoint of protecting a healthy life, and the plan to respond to legal regulations. For this purpose, the technique which exhibits the larger antibacterial effect in the same metal ion concentration is needed.

By the way, as a prior art document of an inorganic type antimicrobial agent, following patent documents 1-3 are mentioned, for example.

In patent document 1, the sterilization technique which uses light is proposed. This technique does not use an antimicrobial metal but sterilizes using a flash pulse of a blue light emitting diode. However, in this technique using strong light, the device deteriorates prematurely, and the light itself may adversely affect the human body.

In patent documents 2 and 3, the technique which sterilizes using a photocatalyst is proposed. Patent document 2 is a technique which sterilizes by irradiating an electromagnetic wave containing a wavelength range of 180-480 nm to photocatalysts, such as a titanium oxide. Patent document 3 is a technique using the antimicrobial acrylonitrile fiber which heat-processes the acrylonitrile fiber which has an antimicrobial active metal compound in the range of pH1-6. However, these techniques cannot realize sufficient antibacterial activity with trace metals.

Patent Document 1: Japanese Patent Application Laid-Open No. 2004-275335

Patent Document 2: Japanese Patent Application Laid-Open No. 11-47738

Patent Document 3: Japanese Patent Application Laid-Open No. 2001-259012

[2nd invention group]

Malignant tumors are serious diseases that threaten human life. As a result, various methods for treating malignant tumors are being developed. The major treatments for malignant tumors include surgical therapy, chemotherapy, radiation therapy and photodynamic therapy (PDT).

Surgical therapy is a therapy that directly removes tumors by surgery, and chemotherapy is a therapy that kills or inhibits the proliferation of tumor cells in the body by administering antitumor agents. Surgical therapy removes the target tumor by surgery, so that the tumor is removed only.

However, it is difficult to surgically remove minute tumor cells that cannot be visually recognized. Moreover, in the case of malignant tumors, since tumor cells (for example, cancer cells) may be displaced to another place, it is difficult to remove malignant tumor cells completely only by surgical therapy. Thus, surgical treatment is generally applied in combination with chemotherapy acting systemically.

Radiation therapy is a therapy that kills tumor cells by irradiating the affected area with a high energy beam. High-energy beams destroy tumor cells and normal cells because of their lack of selectivity. For this reason, only a limited area should be irradiated with a pin point. Therefore, it is not applicable to the non-solid cancer and the micro tumor cell group which is hard to confirm existence. Therefore, radiation therapy is often combined with other therapies, especially chemotherapy.

Photodynamic therapy (PDT) is a therapy in which the laser is irradiated to the affected area after administration of photosensitive chemicals absorbed by somatic cells such as porphyrin derivatives. Photosensitive chemicals, such as porphyrin derivatives, have a property of generating a reactive oxygen by chemical reaction when the laser light is hit. Porphyrin derivatives administered to humans are rapidly lost from normal cells, but remain in tumor cells for a long time. Therefore, after administration of a photosensitive chemical substance using this property and laser irradiation to the affected area after a predetermined time, only tumor cells It is possible to kill selectively.

As described above, in various treatments for malignant tumors, chemicals that exhibit specific actions in vivo have been used, but as a substance having a specific action on malignant tumor cells, platinum compound-based nucleic acid metabolic antagonists are known. have.

Specifically, platinum complexes such as cisplatin (cis-dichloroamine platinum), carboxylic acid platinum, and silicon platinum are known. These platinum complexes all have tetravalent platinum as the center of the structure, and have a structure in which ions such as chlorine, ammonium, carboxylic acid, and silicon are bonded around them. These platinum complexes are believed to kill cancer cells by binding to DNA in cancer cells, inhibiting DNA synthesis and subsequent division of cancer cells, and killing cancer cells, and are effective against solid cancers in which other drugs do not show effective effects. In many cases.

Thus, antitumor agents of the platinum complex are useful antitumor agents. However, the platinum complex system antitumor agent causes severe vomiting in oral administration or intravenous administration, and also causes side effects such as kidney failure. Moreover, a sufficient effect may not be acquired by the difference of the site | part of a cancer occurrence. In addition, there is a problem in that drug resistance occurs (for example, the appearance of cisplatin-resistant cancer) due to long-term use and the effect is lowered.

For this reason, although the treatment which combined the platinum complex system antitumor agent of a different kind, or combined with another antitumor agent is performed, it is not fully solved yet. For this reason, the development of a drug which selectively acts only on tumor cells or a drug which does not act on normal cells but selectively acts only on tumor cells is expected.

As a technique for selectively killing only tumor cells, for example, Patent Documents 2-1 and 2-2 are given below.

Patent Document 2-1: Japanese Patent Laid-Open No. 2004-223175 (claim 1)

Patent Document 2-2: Japanese Patent Application Publication No. 2002-543164 (claim 1)

Patent document 2-1 relates to the tumor treatment apparatus which kills at least a part of the tumor cells which comprise the said tumor by destroying the bubble which exists in a tumor or its surface.

Patent document 2-2 is a method of targeting the delivery of an active agent to a tissue in a target vertebrate, and (a) administering to the target vertebrate before administering a delivery vehicle comprising the active agent. Then exposing the tissue to ionizing irradiation at the time of administration or at the point of combination thereof; And (b) said exposing of said tissue in step (a), thereby targeting said tissue to said delivery vehicle delivery.

However, their technique is not yet sufficient.

[Third invention group]

The automatic refrigerator etc. are normally attached to a household refrigerator. The automatic ice making apparatus provided in the refrigerator generally includes a water supply tank disposed in the refrigerating compartment, an ice making dish provided in one compartment of the freezer compartment, a water pipe for feeding water accumulated in the storage tank to the ice making dish, and iced ice. It consists of a storage container. Although the said water pipe plays a role of watering the water in the water supply tank arrange | positioned in the refrigerator compartment to the freezer compartment side, since the refrigerator compartment and the freezer compartment are partitioned by the heat insulation member, the said water pipe is piped through the heat insulation member. .

In the automatic ice making apparatus of this structure, a part of the water supply tank and the water pipe are arranged in the refrigerating chamber, but the inside of the refrigerator is usually about 1 to 6 ° C., and microorganisms can be grown. For this reason, mold and various germs multiply inside these by the use of a long time.

Therefore, the technique of making these members removable and easy to clean is proposed. As a technique regarding an automatic ice-making apparatus, the technique of patent document 3-1-3-3 is mentioned, for example.

Patent Document 3-1: Japanese Patent Laid-Open No. 5-306858

Patent Document 3-2: Japanese Patent Laid-Open No. 2002-295935

Patent Document 3-3: Japanese Patent Laid-Open No. 2007-333325

[4th invention group]

With the advent of an aging society, there is an increasing opportunity to take care of the elderly and demented elderly who are ill and sick with infants at home. Infants and the elderly who only lie down, the body is very unhygienic, while the resistance to pathogens are weak, high cleanliness is required compared to healthy adults.

In particular, the elderly, who are sick and lying, are expected to sterilize clothing that directly touches the skin, such as clothing and sheets, in order to cure the disease or improve the quality of life.

On the other hand, with the increase of health-oriented, it is also required to be particularly clean about the clothing which a businessman wears.

In such a social background, the role of the washing machine as a tool for keeping clothes clean has increased and increased in the past.

Generally, although sweat and mud adhere to the clothing used for washing, not only these but also many germs and microflora are attached. In addition, significant pathogens such as Staphylococcus aureus, ringworm, etc. may be attached to clothing, sheets (bedding), etc. of a person suffering from a disease.

By the way, the washing | cleaning in a home is often performed with the same washing machine together with miscellaneous clothes. For this reason, microbial contamination may arise between laundry at the time of washing.

In addition, bacteria may remain in the micro-gap spaces present in the washing tank, and the bacteria may multiply until the next washing, thereby contaminating the laundry.

In order to prevent such a thing, the method of using the detergent containing the fungicide conventionally is employ | adopted. Moreover, the method of heat-sterilizing laundry is made into the washing machine at the high temperature of 60 degreeC or more. For example, in patent documents 4-1 to 4-4, the method of adding the metal ion which has antimicrobial property to the rinsing water at the time of washing is proposed.

Patent Document 4-1: Japanese Utility Model Publication No. Hei 5-74487

Patent Document 4-2: Japanese Patent Laid-Open No. 2001-276484

Patent Document 4-3: Japanese Patent Laid-Open No. 2004-215817

Patent Document 4-4: Japanese Patent Laid-Open No. 2004-321313

[First invention group]

MEANS TO SOLVE THE PROBLEM The present inventors earnestly researched in order to solve the said subject. As a result, it was found that the amount of ions absorbed in the microorganism increases by irradiating the microorganisms with specific light. In addition, by irradiating microorganisms with specific light, it was found that eukaryotic organisms with a thick cell wall can also be antimicrobial with a small ion concentration.

The first invention group has been completed based on these findings, and the first invention group has an antibacterial method capable of expressing an antimicrobial effect equivalent to or higher than that of a microorganism at a small ion concentration and a small amount of ions as compared with the prior art, and this method. An object of the present invention is to provide an antibacterial device that can be easily performed.

Here, "antibacterial" in the present specification is not limited to the case where the growth of the microorganism is prevented without killing the microorganism, and "sterilization (killing all microorganisms)", "sterilization (killing a part of microorganisms)", " It is used as a term including all concepts such as disinfection, bactericidal, bactericidal (restraining the growth of microorganisms), mildew, and antiseptic.

The basic structure of the 1st invention group for solving the said subject is the antimicrobial method which uses an ion to prevent the microorganism growth, WHEREIN: After irradiating a microorganism to a specific light or irradiating a ion, the said microorganism is made to contact an ion. And an antimicrobial method for irradiating specific light to the microorganism in a state where ions are brought into contact with the microorganism.

In this configuration, although specific light is irradiated to the microorganism before or while the ion is brought into contact with the microorganism and in contact with the ion, the permeation of the membrane is facilitated by the irradiation of the specific light. As a result, antimicrobial ions present in the vicinity of the microorganisms are efficiently introduced into the microorganisms and exert their effect.

That is, with the above structure, more ions can be introduced into the microorganism in a short time as compared with the conventional method. Therefore, even if the amount of ions to be brought into contact with the microorganism is reduced (one of these means is to reduce the concentration of ions present around the microorganism), the effect of inhibiting the growth of microorganisms equivalent to or higher than the conventional one can be obtained.

In addition, by reducing the amount of ions, antibacterial that does not adversely affect the environment is possible.

In addition, with the above-mentioned structure, since the ion can permeate a cell wall, a cell membrane nucleus membrane, etc. to a eukaryotic organism with a thick cell wall, more ion can be introduce | transduced into a eukaryotic organism, and an eukaryotic organism can be effectively antibacterial. In addition, the said ion means both an inorganic type ion and organic type ion.

Here, the basic principle of the present invention resides in that ions are efficiently introduced into the microorganism after irradiating specific light to the microorganism to increase ion permeability into the microorganism. However, in a practical application scene, even if ions are brought into contact with microorganisms prior to light irradiation, practical problems do not occur. This is because when ions come into contact with microorganisms, ions are present on the surface of the microorganisms, but ions on the surface of the microorganisms do not completely block specific light. Therefore, even when irradiating specific light to the said microorganism in the state in which ions exist in the surface of a microorganism, the specific light which reached | attained the microorganism changes the attribute of a microorganism, and improves ion permeability. This is because the ions (preliminarily contacted ions) previously present on the surface of the microorganism are accelerated and introduced into the microorganism.

In the above basic configuration, the ions may be metal ions.

Metal ions have high antibacterial activity. Therefore, it is preferable to use a metal ion as said ion.

In the above basic configuration, the specific light may be light acting on an ion permeable channel of the biofilm.

By adopting this configuration, the ion permeation channel of the microorganism is opened by the irradiation of specific light, and thus the membrane permeation of the ions is facilitated. As a result, ions in contact with the microorganisms are efficiently introduced into the microorganisms and exert their action on the microorganisms in the microorganisms.

Here, the "ion membrane permeation channel of a biological membrane" mainly refers to a membrane transport protein membrane of a microbial biological membrane, but is not limited to this, and is used as a concept including all mechanisms involved in the introduction of microorganisms.

In the said basic structure, it can be set as the structure whose said metal ion is metal ion contained in a metal ion containing solution. In this configuration, the microorganism is brought into contact with a metal ion-containing solution containing metal ions. In this way, metal ions can be easily and reliably contacted with microorganisms.

In the said basic structure, it can be set as the structure whose said specific light is light which has a peak wavelength in the range of 300 nm or more and 600 nm or less.

If the peak wavelength of the specific light is larger than 600 nm, ions cannot be efficiently introduced into the microorganism. On the other hand, if the thickness is less than 300 nm, since the light itself destroys the DNA of the microorganism, the adverse effect on the human body is large and the deterioration of the device is accelerated. Therefore, it is desirable to regulate within the above range.

In the said structure, it can be set as the structure whose irradiation intensity | strength of the said specific light is 500-500,000 mW / cm <2>.

If the irradiation intensity of a specific light is less than 500 mW / cm 2, ions cannot be sufficiently introduced into the microorganism. On the other hand, even if it is larger than 500,000 mW / cm 2, the effect of introducing ions into the microorganism reaches an upper limit, resulting in poor cost performance. Therefore, it is preferable to regulate within the said range, More preferably, you may be 1000-100,000 m <3> / cm <2>.

Moreover, as a metal ion used by the 1st invention group, it is preferable to set it as 1 or more types of ion chosen from the group which consists of silver ions, copper ions, and zinc ions from a viewpoint of reducing an antibacterial effect and an influence on an environment.

In addition, it is preferable to make the concentration of the metal ion in the said metal ion containing solution into 10 ppm or less from said viewpoint, and to be 1 ppm or more in order to acquire the minimum effect. More preferably, it is 5 ppb-1 ppm, More preferably, it is 10 ppb-900 ppb.

As microorganisms to which the invention of the first invention group can be applied, eukaryotic organisms such as E. coli, photosynthetic bacteria, lactic acid bacteria, actinomycetes, prokaryotes such as Staphylococcus aureus, yeasts such as Candida, Cladosporium and ringworm It can be illustrated.

The present invention relates to an antibacterial device capable of carrying out the antimicrobial method according to the first invention group, the ion generating means for generating ions, the contacting means for bringing ions generated by the ion generating means into contact with a microorganism, and the contacting. It is an antimicrobial apparatus provided with the irradiation means which irradiates a specific light to the said microorganism in the state which the ion contacted the microorganism by a means.

Moreover, the invention which concerns on another form of antimicrobial apparatus is the ion generating means which generate | occur | produces an ion, the irradiation means which irradiates a specific light to microorganisms, and the microorganism to which the said specific light was irradiated was generated by the said ion generating means. An antimicrobial device having contact means for bringing ions into contact.

Here, it can be set as the structure whose said specific light has the peak wavelength in the range of 300-600 nm, and is the light whose irradiation intensity is 500-500,000 mW / cm <2>.

Moreover, it can be set as the structure whose said ion is a metal ion.

Moreover, it can be set as the structure whose said metal ion is silver ion.

By using the antimicrobial device composed of these structures, the microorganisms can be effectively antibacterial by simple operation, and antimicrobial can be performed with very low concentration of ions. Therefore, according to the present invention, substantially no environmental pollution can be generated, and antibacterial objects can be achieved.

According to the present invention, it is possible to remarkably enhance the antimicrobial action against microorganisms without increasing the amount of antimicrobial ions contained in the antimicrobial agent.

[2nd invention group]

The invention of the second invention group has been made in view of the above, and its main object is to provide a technique capable of selectively acting only on tumor cells. The present inventors earnestly researched based on the reader's idea for this purpose. And the following knowledge was obtained.

(1) When specific light is irradiated to a cell, more ion can be introduce | transduced into a cell compared with the case where light is not irradiated.

(2) When the cell is not irradiated with specific light, extraneous metal ions cannot be sufficiently introduced into the cell.

(3) The effect by irradiating a specific light to a cell is remarkably recognized in malignant tumor cells.

The 2nd invention group was completed based on such knowledge. A first object of the second invention group is to provide a method capable of controlling cell activity. More specifically, the present invention provides a method for efficiently and selectively introducing ions only into living cells to be targeted, thereby providing a method for controlling cell activity that inhibits or enhances cellular activity of a target cell. The 2nd objective of 2nd invention group is to provide the cell activity control apparatus which can implement this method easily.

The method and apparatus for controlling cell activity according to the second invention group can be applied to cancer patients, for example. By applying the present invention, metal ion species having antitumor action can be efficiently introduced only into malignant tumor cells. This selectively kills only malignant tumors and reduces side effects.

The 2nd invention group invention which concerns on the cell activity control method for solving the said subject is a method of controlling cell activity using an ion, Contacting the ion to the said cell, after irradiating a specific light to a cell or irradiating it And irradiating specific light to the cells in a state where ions are brought into contact with the cells.

In this constitution (hereinafter, referred to as a basic constitution), specific light is irradiated while the cell is brought into contact with or after contact with the ion, and the cell is irradiated with specific light while the cell is in contact with the ion. When irradiated with specific light, the permeation | transmission of ion membrane becomes easy. Thus, the ions contacted are efficiently introduced into the cell.

That is, with the above structure, more ions can be introduced only into the cells present at the site irradiated with light. Since ions introduced into cells directly affect cell metabolism, by selecting ionic species to be introduced, it becomes possible to enhance cell proliferation or suppress cell proliferation. That is, according to the above configuration, it is possible to control the cell activity.

In the method of the second invention group of the above-described basic structure, the cells can be used as animal cells. In addition, the control of the cell activity in the above-described basic structure is such that after the irradiation of a specific light to an animal cell or the irradiation of the cell, the animal cell is brought into contact with an ion that lowers the activity of the animal cell into the animal cell. Increasing the amount of introduced can be controlled to suppress the proliferation of the cells.

The above-described effects of the invention of the second invention group are more effectively exhibited in animal cells. This technical significance is demonstrated using tumor cells as an example.

In general, tumor cells, particularly malignant tumor cells, are active (proliferative in a state of high cellular activity), and therefore irradiation with a specific light accelerates the introduction of ions and significantly increases the action of the introduced ions on the living body. Expression. In other words, when anti-tumor ions are applied to the affected area while irradiating or irradiating a specific light, anti-tumor ions are introduced at high concentration into tumor cells, thereby acting to inhibit cell proliferation in the cells. On the other hand, the cells present in the region to which specific light does not directly reach have no introduction of anti-tumor ions or the amount thereof is small even if introduced, so that cell proliferation is not suppressed. In addition, even in a region where specific light is irradiated or the like, since normal cells have less amount of anti-tumor ions introduced than malignant tumor cells, normal cells are not killed.

That is, with this constitution, anti-tumor ions can be selectively introduced only into the tumor cells irradiated with light. Therefore, a remarkable effect is obtained that the killing effect on tumor cells is high and does not damage normal cells (the side effects are small). Moreover, in this structure, since anti-tumor ions can be efficiently introduced into tumor cells by specific light irradiation etc., the application amount of the chemical | medical agent to a affected part can be reduced, and this also reduces side effects.

In addition, the "inhibition of proliferation" in the present specification means not only suppression of cell division, but also suppress the growth of the cells themselves, weaken the immune action, and killing cells as a result of suppression of cell division or development. It is used as a term including killing and the like.

In each said structure, metal ion can be used as said ion. Moreover, as said metal ion, 1 or more types of metal ion selected from the group which consists of silver ion, copper ion, or zinc ion can be used.

Metal ions can be suitably used in terms of stability, and metal ions selected from the group consisting of silver ions, copper ions and zinc ions are particularly preferable in terms of high functionality to cell activity. In addition, the effect of increasing the amount of ion introduction by specific light irradiation is remarkably exhibited in metal ions.

Moreover, in the said structure, metal ion contained in the metal ion containing solution can be used as said metal ion.

With this configuration, ions can be easily brought into contact with the cells. For example, when the metal ion-containing solution is a solution containing the platinum complex described above, the solution can be applied to a tumor-bearing lesion to bring the tumor cells into contact with metal ions having anti-tumor action. By irradiating specific light, only malignant tumor cells of the affected area can be killed or the like.

For example, in the case of gastric cancer, after drinking a solution containing a platinum complex, specific light can be irradiated while visualizing a specific site with a fiber scope having a light irradiation function at its tip. In addition, by using a fiber scope having both a light irradiation function and a solution application function at the tip, light irradiation can be performed while applying a platinum complex-containing solution directly to the affected part (cancer cells), whereby only cancer cells are reasonably It is possible to promote the killing of

In each said structure, the light which acts on the ion permeation channel of an animal cell membrane can be used as said specific light.

By adopting this configuration, the ion permeation channel of the animal cell membrane is opened by specific light irradiation, and the membrane permeation of the ions is facilitated. Therefore, with this structure, since the ion contacted with the animal cell can be efficiently introduced into the cell, the cell activity can be efficiently controlled.

Herein, the ion permeation channel of an animal cell membrane typically means a permeation channel in a substance transport mechanism in which a membrane transport protein is involved, but is not limited thereto. It is used in the meaning including all channels to introduce.

In each said structure, the light which has a peak wavelength in the range of 300 nm or more and 600 nm or less can be used as said specific light.

Light having a peak wavelength in a range of 300 nm or more and 600 nm or less is preferable as specific light because of its excellent effect of enhancing ion permeability in animal cells. Moreover, the irradiation intensity of the said specific light can be 500-500,000 mW / cm <2>.

The enhancement effect of ion permeability in animal cells is also affected by the wavelength of light and the irradiation intensity, but it exhibits the effect sufficiently as long as it is light of the irradiation intensity in the above range.

Moreover, in each said structure, it can be said that the said animal cell is a tumor cell. Since tumor cells have more proliferative activity than normal cells, when tumor cells are contacted with ions after or after irradiation with specific light, the introduction of the ions into tumor cells is significantly accelerated, and the introduced ions proliferate. It expresses its effect more markedly in active cells of activity. Therefore, the method of the present invention is strongly selective for tumor cells and control of cell activity.

Here, tumor cells are benign or malignant, and normal cells are physiological, morphological, and biochemically different. In addition, especially "malignant tumor cell", it refers to the cell which has the property to invade (infiltrate), or transpose, and to augment | invade with other tissues in various parts of an animal body, for example, a cancer cell. Examples of the cancer cells include carcinomas (stomach cancer, colon cancer, lung cancer, liver cancer, breast cancer, etc.) that occur in epithelial cells, sarcomas (osteosarcoma, chondrosarcoma, liposarcoma, and hemangiosarcoma) that occur in non-epithelial cells and hematopoietic cells. And cells forming hematopoietic tumors (leukemia, malignant lymphoma, myeloma, etc.).

In the above configuration, as the ions, ions having a greater proliferative effect on tumor cells than those for normal cells can be used.

Since normal cells and tumor cells have different physiological or biochemical characteristics, even when the same ionic species is applied, the degree of preventing or enhancing the proliferation of normal cells and tumor cells is often different. Therefore, only the proliferation of tumor cells can be selectively controlled if the above-described constitution uses ions having a large proliferative disorder on tumor cells and irradiates specific light.

The 2nd invention group invention which concerns on the cell activity control apparatus for solving the said subject is the light source part which has a peak wavelength in the range of 300-600 nm, and outputs the light whose irradiation intensity is 500-500,000 mW / cm <2>, The said light source part The cell activity control device is provided with the irradiation part which induces the output light to the animal cell, and irradiates the said animal light to this animal cell, and the contact part which contacts an ion cell with an animal cell.

According to this configuration, light having a peak wavelength in the range of 300 to 600 nm and an irradiation intensity of 500 to 500,000 mW / cm 2 outputted from the light source unit is guided to the animal cell by the irradiation unit and irradiated with light. This opens the ion permeable channel of the animal cell. The contact portion also contacts the animal cells with ions. As a result, ions can be efficiently introduced into animal cells. Therefore, according to the device having this configuration, it is possible to control the activity of the animal cells simply and quickly.

According to the second invention group invention, a remarkable effect of efficiently introducing ions into cells, for example, human malignant tumor cells, is obtained. Therefore, by appropriately selecting the ionic species to be introduced and specific light, cellular activities such as tumor cells can be arbitrarily controlled, and for example, only cancer cell proliferation can be selectively suppressed.

[Third invention group]

In a refrigerator provided with an automatic ice maker, it is necessary to remove the water supply tank from time to time to replenish water. Therefore, since it is good to wash by the steam which replenishes water, washing is easy to be performed. However, it is a burden for the general user that the water pipe attached to the refrigerator is removed, cleaned, and reinstalled again. In addition, since the inside of the water pipe is difficult to visually recognize from the outside, there is a problem that the cleaning is neglected to become unsanitary.

By the way, it is known from the past that metal ions, such as silver and copper, have antimicrobial activity with respect to microorganisms, such as a mold. However, since metal ions having antimicrobial activity are substances having biological activity, they also affect the human body and the natural environment. Therefore, when using these metal ions for antibacterial use, it is necessary to reduce the usage amount as much as possible.

MEANS TO SOLVE THE PROBLEM As a result of earnestly researching the method of controlling the growth of microorganisms, such as a mold, and the flora and fauna cells, the present inventors found that the ion (especially metal ion) which has antimicrobial activity remarkably enhances the effect when specific light exists. I found out.

The invention of the 3rd invention group is invention completed based on the said knowledge. An object of the third invention group is to provide an ice making apparatus having an automatic antibacterial treatment function capable of always keeping the inside of an ice making apparatus of a refrigerator provided with an automatic ice making apparatus.

The invention of the 3rd invention group for solving the said subject is comprised as follows.

A refrigerator equipped with an automatic ice maker, wherein the automatic ice maker includes: an ice maker, a water supply tank, a water pipe for guiding water accumulated in the water supply tank to the ice maker, an inside of the water supply tank, and And / or an ion supply unit for supplying ions to the water pipe, and a light source unit for irradiating specific light in the water supply tank and / or the water pipe.

Furthermore, in the refrigerator provided with an automatic ice-making apparatus, the said automatic ice-making apparatus is equipped with an ice-making part, the water pipe which guides water to the said ice-making part, and the ion supply part which supplies an ion to the said water pipe, And a light source unit for irradiating the water pipe.

In addition, in the refrigerator provided with an automatic ice-making apparatus, the said automatic ice-making apparatus is equipped with the ice-making part, the water pipe which guides water to the said ice-making part, and the light source part which irradiates a specific light to the said water pipe, And a silver ion eluting material which elutes silver ions.

The refrigerator further includes a refrigerating compartment and a freezing compartment separated from the refrigerating compartment, and further comprising an automatic ice making apparatus, wherein the automatic ice making apparatus includes a water supply tank disposed in the refrigerating compartment and an ice making dish provided in one section of the freezing compartment. And a water pipe for guiding the water accumulated in the water supply tank to the ice tray, and an ion supply unit for supplying ions into the water supply tank and / or into the water pipe tank, wherein the water pipe is light transmissive, The light source part which irradiates a specific light to a water pipe is provided in the vicinity of the said water pipe, It is characterized by the above-mentioned.

In the refrigerator provided with the automatic ice-making apparatus of this structure, furthermore, the water supply tank of the said automatic ice-making apparatus is light-transmissive, and the light source part which irradiates a water pipe to a specific light is provided in the vicinity of the said water supply tank. can do.

Moreover, it can be set as the structure whose said specific light is light which has a peak wavelength in the range of 300 nm or more and 600 nm or less.

Moreover, the irradiation intensity of the said specific light can be set as 500-500,000 kPa / cm <2>.

In the automatic ice making apparatus of these structures, ion is supplied in an inside of a water pipe, a water pipe, and a water supply tank by an ion supply part, and the above-mentioned specific light is irradiated by a light source part. Thereby, microorganisms etc. can be prevented from propagating in a water pipe, or in a water pipe and a water supply tank with low concentration of ions, and can also kill the microorganisms propagated.

That is, in the automatic ice-making apparatus of the said structure, the inside of a water pipe etc. are automatically antibacterial processed. Therefore, even if the water pipe and the water supply tank are not removed and cleaned, the water vicinity of the ice making apparatus is always kept clean, but such an effect can be realized with very low concentration of ions. Such an effect can be obtained as long as it is the said structure, because the said structure employ | adopts the antibacterial method which combines specific light and an ion. The principle of this antimicrobial method is to irradiate a particular light to a microorganism or the like, thereby opening up a function (blocking an ion permeation channel) to block foreign substances inherent in a tissue of a microorganism or the like (including an animal or a plant), thereby The point is to efficiently introduce it into the body. When more ions are introduced into the microorganism, the ions sufficiently express an action such as metabolic disorder, so that growth of microorganisms and the like is inhibited and killed.

In the above configuration, the ion supply unit may be a silver ion supply unit supplying silver ions.

Since silver ion has especially strong antibacterial effect among ions, the said effect is remarkably exhibited.

Moreover, the said silver ion supply part can be set as the structure which is a silver ion supply part which supplies the silver ion aqueous solution whose silver ion concentration is 100 ppb or more and 1100 ppb or less.

When 100 ppb or more and 1100 ppb or less silver ion aqueous solution are used, antimicrobial effect will be acquired by light irradiation of a short time. In addition, if it is this concentration, it is easy to set it as the density | concentration which does not affect a human body by water washing in a water pipe.

Moreover, the said silver ion supply part can be set as the structure which is a silver ion supply part which supplies the silver ion aqueous solution whose silver ion concentration is less than 100 ppb.

Since an aqueous solution containing an extremely low concentration of silver ions having a silver ion concentration of less than 100 ppb can also be used for beverages, if this structure is used, the safety in the case where no water pipes or the like is washed with water after the antibacterial treatment is high.

Moreover, the said silver ion supply part can be set as the structure which supplies the silver ion aqueous solution whose silver ion concentration is less than 100 ppb directly to the said water pipe.

Since the water pipe is not easy to be cleaned, it is easy to be unsanitary, and therefore, the antibacterial property of the water pipe is particularly required. Moreover, since the water pipe capacity is exceptionally small compared with the water supply tank, since this structure can reduce the absolute amount of silver ions, it is easy to ensure safety.

Moreover, the irradiation time of the said specific light can be set as 30 minutes or more.

Even in extremely low concentration silver ions of about 60 ppb, an antibacterial effect is obtained when the light irradiation time is 30 minutes or more.

Moreover, in the said structure, it can be set as the structure which the said silver ion supply part has a cartridge filled with silver ions.

A cartridge filled with silver ions may be mounted at the time of antibacterial operation, and if the amount of silver ions to be filled in the cartridge is defined, the amount of silver ions supplied to the inside of the water pipe can be easily controlled.

The cartridge may have a silver ion aqueous solution having a silver ion concentration of less than 100 ppb, and may be configured such that the irradiation time of the specific light is 30 minutes or more.

If it is this structure, sufficient antibacterial effect can be acquired, ensuring the safety of drinking water.

Also in the third invention group, the term "antibacterial" is not limited to the case where the proliferation is prevented without killing microorganisms, diatoms, etc., "sterilization (killing all microorganisms)", "sterilization (microorganisms) It is used as a term including all concepts such as "killing a part", "" disinfection "," bacterial "," bacterial (prevention of microbial growth) "," fungus "," preservative "and the like.

[4th invention group]

However, since clothing is directly in contact with the skin, strong fungicides are difficult to use.

In addition, since clothing is used repeatedly after washing, heat sterilization methods that damage color or texture are difficult to use.

On the other hand, the techniques described in Patent Documents 4-1 to 4-4 are excellent in that they can be sterilized and washed without damaging clothes. There is a possibility of affecting. For this reason, there exists a problem that it is difficult to fully raise metal ion concentration.

MEANS TO SOLVE THE PROBLEM The present inventors earnestly research about the method of controlling the growth of microorganisms, such as a mold and a bacterium, and the effect that the effect of an ion (especially metal ion) which has antimicrobial power remarkably enhances when a certain light exists. Found. In addition, in the research of the rotary drum type washing machine, mold or bacteria are easily propagated on the rear surface side of the rotating drum, but the ions are brought into the rear surface side of the rotating drum by bringing ions into contact with the back side of the rotating drum while irradiating specific light. We knew that we could prevent mold and bacteria.

The 4th invention group is invention completed based on these knowledge. An object of the invention of the fourth invention group is to provide a rotary drum type washing machine capable of preventing microbial contamination (contamination in a washing machine) during washing and antibacterial treatment of laundry.

The basic structure of the 4th invention group for solving the said subject is as follows.

At least a water tank capable of receiving water and discharging the received water, a rotating drum disposed in the water tank and rotationally driven in the water tank, and ion supply means for supplying ionized water in the rotating drum and / or into the water tank; In the rotary drum type washing machine provided, the rotary drum has a plurality of holes through which water can travel between the rotary drum and the inside of the water tank on the wall surface, and the water tank has an outer side of the rotary drum on its inner surface. It is characterized by including the 1st irradiation part which irradiates a specific light to the side surface.

In this configuration, since ionized water is supplied into the water tank and / or into the rotating drum by the ion supply means, antibacterial treatment with ions is performed on the laundry contained in the rotating drum.

In addition, since a plurality of holes through which water flows are formed in the rotating drum, the ions supplied by the ion supply means are spread evenly in the space between the rotating drum and the water tank, and the outer surface of the rotating drum is wet with ionized water. Shall be.

Therefore, according to this structure, while carrying out ion antibacterial treatment to laundry and ion-antibacterial treatment also to the outer surface of a rotating drum, specific light is irradiated to the outer surface of a rotating drum by a 1st irradiation part. This specific light enhances the antibacterial action of the ions. Thereby, germination of the outer surface (rear surface) of the rotating drum which becomes a hot phase such as mold or various bacteria is achieved.

Here, the structure which irradiates specific light to the rotating drum outer surface wet with ionized water becomes the application of the antimicrobial principle which irradiates a specific light to the said microorganism, contacting an ion with a microorganism. According to this principle, the ion permeation channel of the microorganism can be opened to efficiently introduce ions into the microorganism. Thereby, the antimicrobial activity of ions can be remarkably enhanced. In addition, said "outer surface of a rotating drum" is used by the meaning containing the side surface and the bottom surface of a rotating drum.

The said basic structure can further be set as the structure in which the said rotating drum is provided with the reflecting area which reflects the said specific light on the outer surface.

In this structure, the light irradiated to the outer side of a rotating drum by a 1st irradiation part is reflected in the reflection area provided in the outer side of the rotating drum, and is irradiated to the inner side of a water tank. Therefore, with this structure, not only the outer side surface of a rotating drum but also the inner side surface of a water tank can be reliably disinfected.

Moreover, the said reflective area can be set as the strip | belt shape in the direction orthogonal to the rotation direction of a rotating drum.

If the reflecting region is formed in a band shape in a direction orthogonal to the rotational direction of the rotating drum, the drum rotates, so that light can be spread evenly over the entire inner surface of the water tank facing the rotating drum in a small reflecting region area.

Moreover, the said reflection area | region can be set as the structure formed in spiral shape with respect to the rotating direction of a rotating drum.

If the reflecting region is a spiral reflecting region with respect to the rotational direction of the rotating drum, it is possible to spread light evenly over the entire inner surface of the water tank opposite to the rotating drum.

In addition, the reflection region is formed by being dotted with an island shape on the outer surface of the rotating drum, and the shape of each reflection region is convex.

If the reflecting region is convex or island-shaped, less reflecting material ends. In addition, the light is diffusely reflected by the rotation of the drum to spread the light evenly to every corner of the inner surface of the water tank, which is the opposite surface. Therefore, it is possible to enhance the antimicrobial activity of the entire tank.

Instead of the convex shape and the island shape, a simple island shape may be used, and the convex shape and island shape reflection areas may be arranged in a spiral shape with respect to the rotational direction of the rotating drum.

Moreover, in the said rotary drum type washing machine of each said structure, the said light irradiation part consists of the 1st irradiation part provided in the inner side surface of the said tank, and the 2nd irradiation part provided in the inner bottom surface of the said tank, The said reflection area is rotated It can be set as the structure provided in both the side surface and the bottom surface of a drum.

With this configuration, antibacterial treatment of the entire outer surface of the rotating drum including the bottom surface of the rotating drum and the entire inner side of the water tank, that is, the entire washing tank can be performed.

In the said basic structure, the said specific light can be made into the light which has a peak wavelength in the range of 300 nm or more and 600 nm or less.

Moreover, the irradiation intensity of the said specific light can be 500-500,000 mW / cm <2>.

Moreover, the said ion supply means can be made into the silver ion supply means which supplies silver ion water.

The silver ion supply means may supply silver ionized water having a concentration of 30 ppb or more and 1100 ppb or less.

Although the rotary drum type washing machine which concerns on this invention applies the antimicrobial method which combines a specific light and ion, the principle of this antimicrobial method is that a microorganism etc. (including a flora and fauna) is made by irradiating a specific light to a microorganism etc. Although the function of blocking the foreign matter originally possessed by the tissue is opened (opening the ion permeation channel), the ion permeation channel of the microorganism is 300 nm or more and 600 nm or less. It is thought that it opens effectively by irradiation of the light which has a peak wavelength in a range. In other words, the effect of enhancing the antimicrobial activity is large in the wavelength range.

Moreover, if light intensity of 500-500,000 mW / cm <2> is sufficient, an antimicrobial activity enhancement effect will fully be acquired. Moreover, silver ion has strong antimicrobial activity, and the said antimicrobial activity enhancement effect is exhibited remarkably. Moreover, when the silver ion concentration supplied from the said silver ion supply means is 30 ppb or more and 1100 ppb or less, since the load to a natural environment is small, it is preferable.

In addition, although light of less than 300 nm exhibits an antibacterial enhancing effect, ultraviolet light of less than 300 nm is not preferable because it may deteriorate the member of the washing machine or damage the laundry.

In the basic configuration, the rotary drum type washing machine includes washing operation control means for controlling the washing operation, and the washing operation control means supplies supply of silver ion water from the silver ion supply means at the time of washing the laundry. It can be set as the structure which controls the said silver ion supply means so that it may be performed.

Since a small amount of water is enough in a rinsing process rather than a washing process, a sufficient effect is acquired with a small amount of silver ionized water if it is a method of adding silver ionized water in a rinsing process. Moreover, if it is a method of adding silver ionized water in a rinsing process, silver ion will coat | cover the fiber of laundry by the subsequent drying process. Silver ion-coated clothing can maintain its long-term cleanness since the antibacterial effect lasts for a long time.

In the above configuration, the washing operation control means may control the light irradiation unit so that the irradiation of the specific light is performed for 30 minutes or more.

When silver ions are contacted while irradiating specific light to black mold, staphylococcus, etc. for 30 minutes or more, the antibacterial effect by silver ions becomes remarkably high.

In the said basic structure, the said rotating drum type washing machine is provided with the door which closes the opening of the said tank, and it can be set as the structure with which the 3rd irradiation part which irradiates a specific light in a rotating drum is provided. .

Moreover, in this structure, the said specific light is light which has a peak wavelength in the range of 300 nm or more and 600 nm or less, and it can be set as the structure whose irradiation intensity of the said specific light is 500-500,000 mW / cm <2>.

In this configuration, since specific light is irradiated into the rotating drum, the sterilization of the laundry contained in the rotating drum proceeds efficiently by the action of silver ions and the specific light. In addition, when cleaning the washing tank (rotary drum + water tank) itself, the antibacterial in the rotating drum can be efficiently performed.

The 2nd basic structure of 4th invention group is comprised as follows.

A water tank capable of accommodating water and discharging the water contained therein, a rotating drum disposed in the water tank and rotationally driven in the water tank, and silver ion supply means for supplying silver ionized water in the rotating drum and / or into the water tank. And at least the rotary drum has a plurality of holes through which water can flow between the rotary drum and the tank, and an inner wall surface of the rotary drum is formed with a baffle for rolling laundry. The rotating drum type washing machine in which the light irradiation part which irradiates light in a rotating drum is built in the inside.

According to this configuration in which the light irradiation part is built in the baffle provided in the rotating drum, the distance between the light source and the laundry or the inner wall of the rotating drum becomes closer. Therefore, the efficiency of light irradiation is good and the antibacterial effect becomes high by that much.

In this configuration, the inner side surface of the water tank is provided with a first irradiation unit for irradiating light to the outer peripheral surface of the rotating drum, and the inner bottom surface of the water tank irradiates light to the outer bottom surface of the rotating drum. It can be set as the structure provided with a 2nd irradiation part, and the reflection area is provided in both the outer peripheral surface and the outer bottom surface of the said rotating drum. Moreover, the said light is light which has a peak wavelength in the range of 300 nm or more and 600 nm or less, and it can be set as the structure whose irradiation intensity of this specific light is 500-500,000 mW / cm <2>.

With this structure, the whole washing tank is disinfected with antibacterial treatment of the laundry.

In addition, bactericidal is mainly used in the sense of reducing the number of microorganisms such as mold and various bacteria, and "antibacterial" includes bactericidal and inhibits the growth without killing the microorganism, or "sterilization ( Killing all microorganisms "," sterilization (killing at least some microorganisms) "," disinfection "," bacterial "," bacterial (prevention of microbial growth) "," fungus "," preservative ", etc. It is used in the broadest sense.

According to the present invention, there is provided a rotary drum type washing machine having an automatic antibacterial treatment function capable of disinfecting clothes without damaging the clothes, keeping the inside of the washing tank clean at all times, and without harming the natural environment. Can provide.

BRIEF DESCRIPTION OF THE DRAWINGS The figure explaining the antibacterial method which concerns on a 1st invention group.

Fig. 2 shows the antibacterial test results when Cladosporium cladosporioides (NBRC 6348) was used.

The figure which shows the antibacterial test result in the case of using Escherichia coli (NBRC-3972).

4 is an antimicrobial test result illustrating the relationship between the wavelength of specific light and the antimicrobial effect.

5 is a graph showing the antimicrobial test results of a fifth experimental group.

6 is a graph showing the antimicrobial activity for each group of the same light irradiation intensity.

7 is a graph showing the relationship between the wavelength of irradiation light and the antimicrobial effect.

8 is a graph showing the antimicrobial results of Samples A to D in the eighth experimental group.

9 is a graph showing that a site for introducing silver ions in a microorganism is changed by irradiation with specific light.

10 is a conceptual diagram of an apparatus for generating metal ions.

11 illustrates a cell activity control method according to the second invention group.

12 is a conceptual diagram of a cell activity control device according to the second invention group.

Fig. 13 is a conceptual diagram illustrating an automatic ice making apparatus according to the third invention group example 1;

14 is an enlarged conceptual view for explaining a light irradiation method to a water pipe.

FIG. 15 is a conceptual diagram for explaining an automatic ice making apparatus according to a third invention group example 2. FIG.

FIG. 16 is a diagram illustrating a water pipe covered with a light guide layer. FIG.

FIG. 17 is a conceptual diagram illustrating an automatic ice making apparatus according to Embodiment 3 of the third invention group. FIG.

18 is a graph showing the results of the antibacterial effect confirmation experiment 1.

19 is a graph showing the results of the antibacterial effect confirmation experiment 2.

20 is a schematic sectional view of a rotating drum type washing machine according to Embodiment 4 of the fourth invention group.

Fig. 21 is a schematic sectional view of a rotating drum type washing machine according to the fourth invention group example 2;

It is a cross-sectional schematic diagram of the rotating drum type washing machine which concerns on Example 3 of 4th invention group.

The perspective view which looked at the rotating drum which concerns on 4th invention group Example 3 from the outer bottom surface direction.

FIG. 24 is a cross-sectional view taken along the line X-X in FIG. 22, illustrating a light source disposed on the peripheral wall of the cylindrical water tank. FIG.

It is a schematic diagram which shows the form of the reflection area | region provided in the outer surface of the rotating drum.

It is a schematic diagram which shows the structure of a 1st irradiation part and a 2nd irradiation part.

27 is an enlarged perspective view of a baffle portion of the rotary drum type washing machine according to the fourth invention group example 4. FIG.

28 is a schematic cross-sectional view of a light irradiation part incorporated in the baffle.

It is a graph which shows the bactericidal effect on the laundry in the 4th invention group identification test 1 using Candida albicans.

30 is a graph showing the bactericidal effect in each part of the washing tank of the fourth invention group identification test 1 using Candida albicans.

Fig. 31 is a graph showing the bactericidal effect on laundry in the fourth invention group identification test 2 using Cladosporium cladosporioides.

Fig. 32 is a graph showing the bactericidal effect on laundry in the fourth invention group identification test 3 using Escherichia coli.

[Description of the code]

1, 212: Metal plate (silver plate)

2: metal ion container

3: fungus solution

4 light irradiation unit (LED)

5, 209: Dark Box

6: agar badge

7, 8: test container

9: current source

10: solution for eluting metal ions

11: metal ion generating device

12: test solution

13 metal ion application apparatus (contact part or irradiation part)

201: metal ion container

202: metal plate

203 solution

222: DC power

204 cells

205, 206: Chalet

207: light source

208 ionic solution in contact with the cell

211: metal ion generating container

213: applied voltage

214: ion-light carrier tube

215: Syringe Branch

216: light source

217: Applicable part (cell)

218: fluorescence detection unit

219: computer

220: first control unit

221: second control unit

301: Water Tank

302: water pipe

303: Ice Tray

304: storage container

305: heat insulation member

306: silver ion supply unit

307, 315: electromagnetic valve

308: light source A

309, 310, 311, 313: reflector

312 light source B

314: pump

316, 317: three-way branch furnace with electromagnetic valve

318: drain

319: light guide layer coating water pipe

320: light guide layer

400: rotating drum type washing machine with antibacterial treatment

401: outer frame

402: tank

403: Rotating Drum

404: motor

405: Washing Machine Door

406: water supply

407: drainage

408: hole

409 silver ion supply unit

410: light source (first irradiation unit)

411: light source (second irradiation unit)

412 baffle

415: bottom surface of the rotating drum

430: third research unit

431, 461: light source

432, 451, 462: reflector

440, 441: reflection area

450: second investigating unit

452: transparent cover

460: baffle with light irradiation unit

[First invention group]

[Introduction]

Examples of the ions having an antimicrobial action against microorganisms include metal ions, and specifically, silver ions, copper ions, zinc ions, cadmium ions, and mercury ions. Among these metal ions, silver and / or copper are preferred from the high stability to the human body, but the present invention is not limited to specific ions and can be applied to various ions having antibacterial action.

In addition, the microorganisms to which the present invention is applied are not limited to a specific species, but particularly remarkable effects can be expected in microorganisms having specific membrane-binding proteins that selectively pass metal ions.

Examples of the microorganisms to which the present invention is applied include eukaryotic organisms such as E. coli, photosynthetic bacteria, lactic acid bacteria, actinomycetes, and prokaryotic organisms such as Staphylococcus aureus, for example yeasts such as Candida, Cladosporium and ringworm. Fungi) and can also be applied to pathogenic viruses.

In addition, of course, this invention can be applied also to the object containing multiple types.

The membrane-binding protein is a biological membrane having a property of selectively passing ions in accordance with the concentration gradient or potential gradient of ions in and out of the membrane. Examples of such membrane-binding proteins include membrane transport proteins. Membrane transport proteins are normally functionally closed against the external system, open when there is a constant stimulus, and allow specific molecules and ions to permeate. The membrane transport protein has a kind of ion that can be introduced (transported) by its potential selectivity, and according to the kind of the membrane transport protein, ions that can pass through the membrane and ions that cannot pass through Is considered to exist (selectivity arises). In addition, the rate of passage through the membrane varies depending on the type of ion.

The present invention is characterized in that the antimicrobial action of ions is particularly high by irradiating specific light and introducing ions into the microorganism in a short time, but as a light source used in the present invention, an LED (light emitting diode), Solid state lighting, such as LD (laser diode), and tube-shaped illumination, such as a black light and a halogen light source, etc. can be illustrated, Preferably, LED and LD are used. It is because LED and LD can control the wavelength of light, and it is easy to incorporate into an antibacterial device, and also has the advantage that it is advantageous in cost.

What is necessary is just to select suitably the contact time of the ion with respect to a microorganism according to the kind of ion used, the kind of microorganism, ion concentration, and microorganism amount. For example, when E. coli is antibacterial using silver ions, it is usually about 1 minute to 24 hours, preferably about 1 minute to 3 hours.

Embodiment for implementing a 1st invention group is described based on an experiment group.

1, the outline | summary of the experiment procedure in the following experiment groups is shown. In FIG. 1, code | symbol 1 is a metal plate, code | symbol 2 is a metal ion accommodation container, code | symbol 3 is a bacterial solution which disperse | distributed the bacterium, code 4 is a light irradiation part, code 5 is a dark box which consists of a light-shielding material, code 6 is microorganism Agar medium for cultivating the nutrient solution, 7 is a test container, 8 is a test container on the side not irradiated with light, 9 is a current source, 10 is a solution for eluting metal ions. In addition, for the sake of explanation, the whole including the metal plate 1, the metal ion accommodating container 2, the current source 9, and the metal ion elution solution 10 is made into the metal ion generating apparatus 10, and it is dark. The whole including the light irradiation part 4, the test container 7, and the test liquid 12 in the box 5 and the dark box 5 shall be called the metal ion application device 13.

The light irradiation section 4 has a structure in which one or more LEDs (manufactured by Nichia Chemical Industries, Ltd., Cree, etc.) are combined. The metal ion application device 13 functions as a device which also serves as a means (contact part) for bringing metal ions into contact with microorganisms and a means (light irradiation part) for irradiating light to the microorganisms.

(First experimental group)

A silver plate was prepared as the metal plate 1. Silver has a small tendency to ionize, has a characteristic of being difficult to emit electrons and difficult to oxidize, and has a standard monopolar potential of +0.8 V, which is difficult to become a cation. For this reason, even if silver in a metallic state is put in water, it does not elute easily. Therefore, as a preparation method of a silver ion solution, it is good to use the metal ion generating apparatus 11 of FIG. 1 which applies an electric field to silver. Specifically, two pure silver plates are arranged in the metal ion container 2 shown in FIG. 1, and the voltage (about 50 V) is controlled so that a current of 10 to 50 mA is applied between the silver plates. Silver ions were eluted in the solution. According to this method, 900 ppb of silver ion solution can be obtained, for example by energizing for 30 seconds at 12.5 kPa.

In addition, by controlling the current and the application time applied between the silver plates, the silver ion solution having a concentration of 1 to 2000 ppb can be produced using the metal ion generating device 11. In addition, in order to prepare a higher concentration silver ion solution than this, 106.2 ppm of silver ion zeolites (made by NFG) is hold | maintained in 10 mL of aqueous solution, and cation exchanged using silver ion zeolite (made by NFG company), for example. Can produce ionized water.

The silver ion container 2 is preferably made of a material that is inert to a silver ion-containing solution, and examples of such a material include glass, Teflon (registered trademark), stainless steel, and the like.

As microorganisms to be antimicrobial targets, about 1 × 10 ⁷ to about 1 × 10 ⁸ Cladosporium cladosporioides (NBRC 6348) standard strains (eukaryotes) were prepared as initial bacterial counts. The cells were dispersed in phosphate buffer (concentration 50 mM) to prepare a spore suspension (3). As a container for preparing spore suspension, a container such as glass, Teflon (registered trademark) or stainless steel is used.

In addition, a metal ion application device 11 was used as a device serving as a contact portion for contacting microorganisms with metal ions and a light irradiation unit for irradiating the microorganisms with light output from a light source.

Into one of the two test vessels (7 · 8) of FIG. 1, a solution containing 9 mL of 900 ppb silver ion solution and 1 mL of the microbial solution of the spore suspension (3) was placed (this is referred to as test solution A). In (8), a solution obtained by mixing 9 mL of pure water and 1 mL of the bacterial solution of the spore suspension (3) was added instead of the silver ion solution (this is referred to as test solution B). And these test containers are immediately placed in the dark box 5 in order to completely remove the influence of light such as natural light, fluorescent lamps, and then irradiated with ultraviolet light having a peak wavelength of 365 nm and an irradiation intensity of 1800 mW / cm 2. The test solution was collected after 15 minutes of ultraviolet light irradiation, after 30 minutes, and after 60 minutes.

In addition, the code | symbol 4 of FIG. 1 is a light irradiation part, The light source of this light irradiation part has a structure which combined 14 ultraviolet LED 4 (made by Nitride Semiconductor). The ultraviolet light was outputted by applying a voltage of 3.8 V and a total current of 21 mA to the LED 4.

In addition, the test solution A and B separately, a mixture of 9 mL of 900 ppb silver ion solution and 1 mL of the microbial solution of the spore suspension (3), 9 mL pure water (does not contain silver ions) and the spores A test solution containing 1 mL of the bacterial solution of suspension (3) was prepared as Test Solution D. In order to completely remove the effects of light such as natural light or fluorescent lamps, the test vessels of these test solutions are immediately placed in the dark box 5 and allowed to stand without irradiating light, and after 15 minutes, after 30 minutes, after 60 minutes Was taken.

In each of the test solutions described above, the test solution A that satisfies all three requirements of [Bacterium + silver ion + specific light irradiation] is used as the test solution according to the antibacterial method of the present invention.

On the other hand, the test solution B that satisfies only two conditions of [cell + specific light irradiation], the test solution C that satisfies only two conditions of [cell + silver ion], and the [cell] of contact with silver ions and specific light irradiation The test liquid D, which is also not used, is a comparative example.

In the preparation of the respective test liquids, the ultraviolet LED 4 functions as a light irradiation unit for outputting specific light that facilitates the membrane permeation of the ions of the biological membrane, and the metal ion application device allows the metal ion application device to contact the metal ions with the microorganisms. It functions as a contact part and an irradiation part which irradiates light to microorganisms.

100 μL of the sample solution is collected from each of the test solutions A to D prepared above, which is then smeared on the potato agar medium 6 shown in FIG. 1, incubated at 25 ° C. for 7 days, and the number of colonies on the medium is counted. By this, the antimicrobial effect was confirmed. This result is shown in FIG.

From Fig. 2, test solution A (o) in the ultraviolet irradiation in the state in which silver ions were brought into contact with Cladosporium cladosporioides was 1 more than test solution C (in Fig. 2) in which silver ions were contacted without being irradiated with ultraviolet light. It was recognized to exhibit a high order of bactericidal effect.

Moreover, the comparison of test liquid B (▲ in drawing) and test liquid D (Δ in drawing) showed that antibacterial effect was not recognized at all only by irradiation of an ultraviolet light.

From these results, the following can be concluded. The irradiation intensity of ultraviolet light used in the above experiment is sufficiently lower than the strength capable of antibacterial fungi. Therefore, the difference in the antimicrobial activity in Test Solution A (○) and Test Solution C (●) is not due to the sterilization effect of ultraviolet light itself, and this difference is the result of the introduction of metal ions into cells by ultraviolet light irradiation. I think. That is, it is considered that the antimicrobial activity of silver ions increased as a result of the easy incorporation of silver ions into the Cladosporium cladosporioides body by irradiation of light having a peak wavelength of 365 nm and irradiation intensity of 1800 Pa / cm 2.

As is apparent from the above results, the specific light irradiated to the cells is sufficient to have a weak light intensity that does not have an antibacterial effect on the fungus. Therefore, the antibacterial method of the present invention can also be used as a method for antibacterial fungi propagating on the skin of humans or animals. In addition, from the viewpoint of enhancing the safety when applying the antibacterial method of the present invention, it is preferable that the specific light has an irradiation intensity and / or irradiation time such that the light itself does not have an antibacterial effect on the cells.

(Second experimental group)

In place of the Cladosporium cladosporioides (NBRC 6348), except for using Escherichia coli (NBRC-3972, Escherichia coli), four types of test solutions were prepared in the same manner as in the first experimental group, and an antibacterial test was performed on these test solutions. In addition, the test liquid prepared in the 2nd experimental group is made into the test liquid 2A-2D corresponding to the said test liquids A-D.

The antimicrobial test results are shown in FIG. 3. ▲-▲ (test liquid 2A) of FIG. 3 satisfy | fills all three requirements of [cell + silver ion + specific light irradiation], and this corresponds to the example of this invention. On the other hand, (-C) (test solution 2C) in Fig. 3 satisfies only two conditions of [Bacterium + silver ions], and Δ-Δ (test solution 2B) satisfies only two conditions of [Bacterium + specific light irradiation], Since (circle) (test liquid 2D) does not carry out contact of silver ions and specific light irradiation with respect to [microbe], these are the comparative example group with respect to the said example of this invention.

As apparent from the results of Fig. 3, the combination of irradiation of specific light and silver ion contact yielded an E. coli antimicrobial effect of about three orders of magnitude higher than that of contact with silver ions without irradiation of specific light (▲- ▲, ●-●). In addition, the antibacterial tendency in the case where specific light was irradiated but not in contact with silver ions (Δ-Δ), and when specific light was not irradiated and not in contact with silver ions (○-○), was observed in the above fungi ( It was the same as the case in test liquids B-D).

(Third experimental group)

Here, the relationship between the kind of specific light and the antibacterial effect was investigated using three types of specific light having different peak wavelengths of 365 nm, 525 nm, and 660 nm.

Specifically, the same Cladosporium cladosporioides (NBRC 6348) was used as the first experimental group. As a light source, it outputs by the LED unit (peak wavelength is 365 nm, irradiation intensity 1800 mW / cm <2>, peak wavelength 525 nm, irradiation intensity 2800 mW / cm <2>, peak wavelength 660 nm, irradiation intensity 4000 mW / cm <2>, respectively, as a light source. I was. The silver ion concentration was 600 ppb, and the irradiation intensity was 525 nm, and the cells were left standing in phosphate buffer without nutrients for one day (one day starvation). Similarly.

4 shows the antimicrobial test results. From FIG. 4, it was recognized that there exists a high antimicrobial activity enhancement effect in wavelength 365nm ((circle)) and wavelength 525nm ((triangle | delta)). On the other hand, at wavelength 660 nm (◇), the antimicrobial activity enhancement effect was hardly recognized.

It is considered that the antimicrobial enhancing effect is not recognized at a wavelength of 660 nm because silver ions cannot be efficiently introduced into the microorganism in this wavelength light.

From the said result, it is not preferable that it is 660 nm or more as specific light. Therefore, preferably, light whose peak wavelength is less than 660 nm is used, More preferably, light whose peak wavelength is 600 nm or less is used. However, light having a peak wavelength of less than 300 nm is not preferable because it damages DNA of living cells and harms humans and animals. In addition, light of less than 300 nm is not preferable because it causes premature deterioration of the antibacterial device (housing or the like). Therefore, as light used for enhancing antibacterial activity, it is preferable to use light having a peak wavelength of 300 to 600 nm.

(4th experimental group)

Test solution A (Cladosporium cladosporioides specific light irradiation + silver ions) of the first experimental group, Test solution C (no light irradiation + silver ions), Test solution 2A (Escherichia coli specific light irradiation + silver ions) of the second experimental group and test solution 2C ( No light irradiation + silver ion), 60 minutes after the light irradiation, the test solution was collected, and the cells in the test solution were recovered by centrifugation. These cells were pre-fixed in an environment of 4 ° C. using 0.1 M cacodylic acid buffer and 2% glutaraldehyde, and then washed three times with 0.1 M cacodylate acid buffer at 4 ° C., and further, 2% osmium tetraoxide at 4 ° C. Post-fixing treatment was carried out by the method of penetrating into the aqueous solution for 3 hours.

Subsequently, after 10 to 15 minutes of treatment with rising alcohol heat, the mixture was treated with propylene oxide for 10 minutes and three times, and further substituted with propylene oxide + epoxy resin for 1 hour, and then replaced with an epoxy resin (Epon812, a curing agent DDSA). , NMA, accelerator DMP-30) The embedding treatment was carried out at 60 ° C. for 2 days (to allow the resin to penetrate into the biological sample for 2 days (treated temperature 60 ° C.)).

The sample thus obtained was exfoliated using ultramicrotome, reinforced with a carbon film to prevent charge up, and a sample for observing an energy filter type transmission electron microscope (EF-TEM) was produced. This sample was subjected to electron microscopic observation and energy dispersive X-ray analysis using R-TEM SuperUTW manufactured by EDAX Corporation.

As a result, about Sample A regarding Cladosporium cladosporioides, although the crystal | crystallization of silver was not able to be confirmed by the electron microscope observation, it was confirmed that silver was unevenly localized in the specific site | part inside a cell rather than the cell membrane in X-ray analysis. With respect to Sample 2A relating to Escherichia coli, it was confirmed that silver was present in the central portion of the cell, not around the cell membrane. In addition, about sample C and 2C which were not irradiated with light, silver was not detectable inside a cell by microscopic observation and X-ray analysis.

From these results, it is thought that silver ions are introduced into the cells by the specific light irradiation and exhibit an antimicrobial effect. Since the introduction of silver ions is significantly promoted by the irradiation of specific light, a stronger antibacterial effect is obtained. It is thought to express.

(5th experimental group)

In the fifth experimental group, the relationship between the silver ion concentration and the light irradiation intensity, the antibacterial power and the light wavelength on the antimicrobial activity was investigated using the same apparatus and method as described in the first experimental group (see FIG. 1).

As a microorganism targeted for antimicrobial activity, 1 × 10 ⁷ standard Candida albicans NBRC-1060 standard strain (eukaryote) was prepared, and the bacteria were dispersed in phosphate buffer (concentration 50mM) using sterile water. × 10 ⁶ gae / 1mL a bacterium solution was adjusted.

On the other hand, the test solution which prepared the silver ion concentration solution of four different concentrations of 60 ppb, 100 ppb, 300 ppb, and 1100 ppb, and added 1 mL of the said above-mentioned said bacterium solution to 9 mL of these four other silver ion solutions was carried out to the silver ion of the same density | concentration. Three each (four types × 3 pieces = 12 test solutions). In addition, two test liquids (control test liquids) having a silver ion concentration of zero containing only 1 mL of the bacterial solution in 9 mL of the phosphate buffer solution were prepared. In addition, the container similar to the test container 7 shown in FIG. 1 was used as a container to which a test liquid is put.

Next, a test liquid group having the same silver ion concentration is used as a test unit, and one of the three is disposed on the test container 8 side in FIG. 1, and the other two are sequentially tested without ultraviolet light irradiation. One side, irradiated with ultraviolet light having a peak wavelength of 365 nm for 30 minutes at an irradiation intensity of 1 mW / cm 2 (1,000 mW / cm 2), and 50 mW / cm 2 (50,000 mW / cm 2) External light was irradiated for 30 minutes.

In addition, the control test liquid group was irradiated with ultraviolet light of one of them without being irradiated with ultraviolet light (arranged on the side of the test container 8 in FIG. 1), and with the other with ultraviolet light having a strength of 50 kW / cm 2 (FIG. 1 test container). (7) side].

Each test solution was taken 30 minutes after the ultraviolet light irradiation. Take the collected test solution as Samples A to N. Table 1 lists the test conditions of Samples A to N.

In addition, as in the first experimental group, the test solution was immediately stored in the dark box 5 after preparation so that light such as fluorescent lamps and natural light in the laboratory did not affect the test solution.

Antimicrobial tests were performed on Samples A to N. The antimicrobial test method was based on a method of counting the number of colonies on the medium after 100 μL of the collected test solution was smeared on agar medium 6 (potato agar medium) shown in FIG. 1 and incubated at 25 ° C. for 3 days.

The experimental results are shown in FIG. 5. 6, the result shown in FIG. 5 was shown collectively for every light irradiation intensity. Fig. 5 is a graph showing the relationship between the silver ion concentration and the light irradiation intensity on the antimicrobial effect, but in this Fig. 5, Sample A (with no silver ion concentration of 0+ light irradiation) and Sample B (silver ion concentration of 0 + 50 kPa) / Cm 2 light irradiation), it can be seen that the antimicrobial effect does not occur even when irradiated with 365 nm ultraviolet light for 30 minutes at a radiation intensity of 50 mA / cm 2.

Further, the following can be seen from the C to E groups, F to H groups, I to K groups, and L to N groups having the same silver ion concentration and different light irradiation intensities.

(i) In any silver ion concentration, when irradiated with light, an antimicrobial effect becomes high.

(ii) At any silver ion concentration, the stronger the light irradiation intensity, the higher the antibacterial effect.

(iii) The antibacterial activity enhancing effect by light irradiation is particularly remarkable when the silver ion concentration is 1100 ppb.

In addition, from the comparison of sample C (silver ion 1100 ppb + no light irradiation) and sample H (silver ion 300 ppb + 50 mW / cm 2 light irradiation), by irradiation with light, the antibacterial or more of equivalent or more at a silver ion concentration of 1/3 to 1/4 It can be seen that the effect can be obtained.

In Fig. 6, the results shown in Fig. 5 are collectively shown for each light irradiation intensity. The above items can be read more clearly from FIG. 6. That is, only ultraviolet light having an irradiation intensity of 50 kW / cm 2 does not allow bactericidal effects to be recognized. When the silver ion concentration is 1100 ppb, a sufficient antibacterial effect is obtained at a light irradiation intensity of 1 kW / cm 2, and light is obtained at a silver ion concentration of 1100 ppb. It can be seen that the antimicrobial effect is greater in the light irradiation intensity of 50 mA / cm 2 at the silver ion concentration of 300 ppb than in the absence of irradiation.

From the above results, it was confirmed that the antibacterial action of silver ions can be remarkably enhanced by using weak light such that the antimicrobial action does not directly affect cells.

In this experimental group, light irradiation was performed while the cells were immersed in a silver ion solution, but light irradiation may be performed after spraying silver ionized water onto the cells (microorganisms). In addition, silver or silver compounds or silver ion-containing materials (for example, silver ions-containing zeolites, ceramics, silica gel, silicates, calcium phosphates, etc.) are attached to the cells (microorganisms), and then silver is applied to the cells by spraying water. Ions can be contacted.

In addition, even if the cells are previously irradiated with light, and after silver irradiation, silver ions are brought into contact with the cells within 20 minutes, preferably within 5 minutes, and more preferably within 2 minutes, the antibacterial activity enhancing effect by light irradiation is achieved. Obtained.

(The sixth experimental group)

In the 6th experimental group, the relationship of the wavelength of the light to irradiate and antibacterial effect was investigated using the light from which a wavelength differs.

As described above, Candida albicans NBRC-1060 was used as a cell, and ⁶ test solutions containing 7 × 10 ⁶ cells / mL and 1100 ppb silver ion concentration were prepared. One of these was not irradiated with light, except that any one of peak wavelengths of 365 nm, 400, 525, 600 nm, and 660 nm was irradiated for 30 minutes. Thereafter, an antimicrobial test was conducted in the same manner as in the fifth experimental group. As a light source of each light, LED (light emitting diode) was used.

The results are shown in bar graph in FIG. As apparent from the results in Fig. 7, when the wavelength exceeds 600 nm, the light irradiation effect is not recognized. On the other hand, the remarkable effect was recognized at 365 nm, 400 nm, and 525 nm, and the remarkable effect was recognized especially at 525 nm.

From this result, it can be seen that the antibacterial activity of silver ions is enhanced when silver ions are brought into contact with cells (microorganisms) and irradiated with light having a wavelength of 600 nm or less. Therefore, the "specific light" seen from this result turns into the light of wavelength 600nm or less (preferably less than 600nm).

Moreover, from the tendency shown in FIG. 7, there exists an antimicrobial activity enhancement effect also in the light whose wavelength is less than 365 nm. However, since the light whose peak wavelength is less than 300 nm damages the DNA of a living cell, since it may damage an operator which performs this method, it is unpreferable. Therefore, as specific light used for enhancing antimicrobial activity, it is preferable to use light whose peak wavelength is 300 or more and less than 600 nm.

(7th experimental group)

In the seventh experimental group, Escherichia coli IFO3972 was used as cells to investigate the presence or absence of an antimicrobial activity enhancing effect on silver ions, copper ions, and zinc ions. Specific conditions are as follows.

The number of bacteria was 4.7 × 10 ⁷ .

Irradiation conditions were made to irradiate for 30 minutes by the light irradiation intensity of 50 dl / cm <2> using the light of peak wavelength 365nm. As the metal ion concentration, silver ions were 110 ppb, copper ions were 2500 ppb, and zinc ions were 6500 ppb.

As a reference (control test solution), a phosphate buffer (concentration 50 mM) solution without a metal ion was used.

In the production method of the copper ion and zinc ion aqueous solution, 1 g of pure zeolite or zinc zeolite was added to 5 mL of pure water, followed by stirring. After standing for 1 hour, the mixture was centrifuged at 5000 G for 10 minutes, the supernatant was collected, and diluted appropriately. It was based on the method of confirming the metal ion concentration of this aqueous solution by atomic absorbance analysis.

The results in the seventh experimental group are listed in Table 2.

As apparent from the results in Table 2, it was confirmed that the antibacterial effect was increased by light irradiation also in metal ions (copper ions, zinc ions) other than silver ions. In addition, the antimicrobial effect is increased by irradiation of specific light, as a result of the ion transmission channel of the microorganism (microorganism) being opened by the irradiation of specific light, the metal ions present on the surface of the cell is rapidly introduced into the bacteria body. It is considered that this is because the body exhibits a physiological effect (anti-proliferative effect).

(8th experimental group)

In the first to seventh experimental groups, it was described that when microorganisms were irradiated with specific light, the antimicrobial activity was enhanced, and the reason for this was that metal ions present on the surface of the cells were quickly introduced into the cells of the bacteria. The eighth experimental group investigated this in more detail. That is, in the eighth experimental group, the sites where silver ions were introduced into the cells and the presence sites and the ratios of the silver ions introduced into the cells were clarified using an inductively coupled plasma emission spectrometry.

ICP emission spectrometry is an analytical method in which argon gas is flowed into a high frequency coil through which a strong electric current is passed to generate a stable plasma flame at a high temperature of about 9,000 K, and a sample of a solution atomized therein is introduced to produce a light emitting light source. .

As a specimen, cells of Candida albicans NBRC-1060 were used as cells, and cells / water (A, B) and cells / 1100 ppb containing 9.5 × 10 ⁶ cells / mL were tested using two kinds of test solutions of silver ionized water (C, D). Two each was prepared. Then, one of two different kinds of test liquids was not irradiated with light, and the other side was irradiated with light having a peak wavelength of 365 nm for 30 minutes at an irradiation intensity of 50 dB / cm 2. LED (light emitting diode) was used as a light source.

With respect to the specimens A to D prepared above, the silver ion-photocomposite effect was first confirmed using the same apparatus and method as described in the first experimental group. Experimental conditions of each specimen are shown in Table 3 below.

In FIG. 8, the antimicrobial test result in each condition of Table 3 is shown. From the results in FIG. 8, it was confirmed that when silver ions were contacted while irradiating light to the microorganisms (microorganisms), the antibacterial activity was significantly increased as compared with the case where silver ions were contacted without irradiating light.

Next, about the cells of specimens A to D, the site of presence of silver introduced into the cells was clarified by the following experiment.

(Experimental method)

For each sample, the precipitates (cells) centrifuged at 15,000 revolutions / min for 10 minutes were collected using a centrifugal separator cooled at 4 ° C, respectively, and the mitochondria of each sample bacterium was used using Mitochondria Isolation Kit manufactured by PIERCE. It was separated into fractions, nuclear fractions, and cytosol fractions. In addition, cytosol means the part which excluded the intracellular organelle from the cytoplasm.

After accurately weighing the fractions of each specimen using micro balance, 5 mL of concentrated nitric acid (61%; EL: for high purity chemicals for electronics industry) and 25 mL of ion-exchanged water were added, and the total amount was 30 mL. It was set as. 5 mL of these was put into the container for ultrasonication, and the ultrasonic vibration process was performed for about 5 minutes.

Thus, the amount of silver was calculated | required by the ICP analysis method about each sample of each sample microbe prepared. The amount of silver in each fraction sample was performed by performing ICP analysis method on the external standard sample of silver beforehand, preparing the analytical curve, and applying the value in each fraction sample to this calibration curve.

9 shows the results of the ICP analysis. In FIG. 9, the distribution ratio of the vertical axis | shaft makes the total amount of silver amount of each fraction detected in each fraction of mitochondria, nucleus, and cytosol as 1, and the ratio of the amount of silver which exists in each fraction with respect to the total amount 1 ( Abundance). In the case of no water / light and no water / light, silver was not detected from any fraction, and therefore, the distribution ratio was expressed as zero.

(Experiment result)

As shown in Fig. 9, in the cytosol, neither the silver ions / no light nor the silver ions / light was substantially present.

In the absence of silver ions / light, the presence of more silver was recognized in the mitochondrial fraction than in the nucleus.

On the other hand, in the presence of silver ions / light, the presence of more silver was confirmed by nuclear fraction than mitochondria.

That is, it was recognized that the amount of silver ions introduced into the nucleus was about 2.8 times stronger than that of the silver ion / light of the mitochondrial fraction and the presence of silver ion / light in the nucleus. From this result, it became clear that the amount of silver ions introduced into the nucleus increased significantly by irradiation of light (specific light) having a peak wavelength of 365 nm.

From this fact, it can be concluded that the remarkably high antibacterial effect represented by (Ag + 365 nm) in Fig. 8 is due to the large amount of silver ions introduced into the nucleus by irradiation with light having a peak wavelength of 365 nm. Also from this fact, it can be inferred that the antibacterial mechanism will be different in the case of silver ions alone and in the case where silver ions are brought into contact with microorganisms while irradiating specific light.

That is, in the case of silver ions alone, silver accumulates selectively in the mitochondria and causes an energy metabolic disorder, whereas when silver ions are brought into contact with microorganisms while irradiating specific light, the nucleus is not a nucleus. As a result of the introduction of silver into the metal, it is thought that the microorganisms give a strong proliferation disorder.

[2nd invention group]

The second invention group provides a method for introducing more ions into live cells in a short time, and at the same time, provides a cell activity control device to which such a method is applied.

"Ion", which is a component of the second invention group invention, refers to an atom or a group of atoms having a positive or negative charge, and the second invention group invention covers all ionic species. Since ions are highly reactive particles, when ions are put into biological cells, they give some action to living cells. The degree and content of the action depends on the type of ionic species. The kind of the ionic species refers to the difference between metal ions, organic ions, inorganic ions, the difference of constituent elements, and the like apart from the positive and negative ions. In addition, even if the ionic species are the same, the degree of action differs depending on the type of cells to be applied and the amount of ions introduced into the cells. Therefore, by selecting ionic species and introducing more ionic species into biological cells, it is possible to reliably control the cell activity of biological cells.

The cell targeted by the second invention group is a cell having a cell membrane, and as long as the cell membrane has a cell membrane, all of the plant cells, animal cells, microbial cells, bacteria, viruses, and the like are targeted. As described above, ions are selected in accordance with the purpose of application (enhanced or inhibited cell activity, etc.), but from the purpose of suppressing the activity of malignant tumor cells of humans and animals, metal ions (plus ions) useful. As a metal ion excellent in the function which suppresses cellular activity, silver ion, copper ion, zinc ion, cadmium ion, mercury ion, etc. can be illustrated. Among them, silver and / or copper have high safety to the human body and strong action on tumor cells, and are therefore useful for the purpose of suppressing the proliferation of malignant tumor cells.

In addition, the application subject of the second invention group is not limited to a specific kind of cell, but an appropriate application object is an animal cell having a membrane permeable channel for selectively passing metal ions. Animal cells are provided with cell membranes that block the passage of extraterrestrial (extracellular) substances, but when the animal cells are irradiated with specific light, metal ion permeability is significantly increased. Therefore, when the animal cells come into contact with metal ions while irradiating or irradiating a specific light, a remarkable cell activity control effect can be obtained.

2nd invention group invention can obtain the remarkable cell activity control effect especially in malignant tumor cells. Examples of malignant tumor cells to which the second invention group can be appropriately applied include, for example, carcinomas (stomach cancer, colon cancer, lung cancer, liver cancer, breast cancer, etc.) occurring in epithelial cells, sarcomas (osteosarcoma, And tissue cells such as hematopoietic tumors (leukemia, malignant lymphoma, myeloma, etc.) occurring in hematopoietic cells. Moreover, the pathogenic virus can also suppress its proliferation by application of the invention of the second invention group.

A typical type of membrane permeation channel for selectively passing the above metal ions is the permeation channel to which specific membrane transport proteins in the animal cell membrane participate. This transmission channel transports only specific molecules and ions (metal ions) in the cell. Therefore, in the case of permeation involving the membrane permeation channel, there is a metal that can pass through the membrane and a metal that cannot pass through (selectivity) depending on the type of membrane transport protein, and the rate of passage through the membrane depending on the type of metal. Seems to be different.

In addition, the passage speed of the membrane is considered to be related to the difference (concentration gradient or potential gradient) of ion concentrations on the outside and inside of the cell membrane. Such a membrane permeation channel is normally considered to be functionally closed with respect to the external system, open when there is a constant stimulus, and transmit specific molecules or ions.

The second invention group invention is stimulated by specific light to functionally open the membrane transmission channel. However, the present invention is not limited to the transmission channel to which the specific membrane transport protein is involved. The ion permeable channel in the second invention group includes all channels in which the amount of ion introduction into the living cell increases by irradiation of specific light.

The specific light which is a component of the 2nd invention group invention selects the light of the wavelength which can increase the amount of ion introduction into a cell, and / or the rate of ion introduction into a cell according to the kind of cell to which it applies. That is, although specific light should just be determined according to the kind of cell used as an application object, the light which has a peak wavelength in the range of 300 nm or more and 600 nm or less is generally used. Light having a wavelength larger than 600 nm has a small effect of opening the ion transmission channel, and light having a wavelength of less than 300 nm is not preferable because the light itself is likely to damage normal cells. Moreover, since the light of wavelength less than 300 nm may injure an operator, handling property worsens.

What is necessary is just to determine suitably the irradiation intensity of the light of a specific wavelength according to the state of the cell to which it applies, and the purpose of cell activity. Generally, the strength is in the range of 500 to 500,000 mW / cm 2. If the light irradiation intensity is 500 mW / cm 2 or more, the ion permeation channel of the animal cell membrane is stimulated, and even if the light irradiation intensity is greater than 500,000 mW / cm 2, the effect on the ion permeation channel reaches the upper limit. Because it gets worse.

As the light source for generating the specific light, solid state lighting such as LED (light emitting diode) and LD (laser diode), or bulbous lighting such as black light or halogen light source can be used. use. LEDs and LDs can define wavelengths of light, and are easily incorporated into a device for controlling cell activity (cell activity control device), which is advantageous in terms of cost.

About the light irradiation time to a cell, what is necessary is just to set suitably according to the kind of ion to be used, ion concentration, cell type, and cell volume. For example, when silver ions are used to suppress proliferation of squamous cell cells derived from laryngeal cancer, the light irradiation time is about 1 minute to 1 hour, for example, about 5 to 40 minutes, or about 10 to 20 minutes. Investigate.

In addition, specific light can be delivered to the application target site (cell) using an optical transmission device such as an optical fiber or the like. In addition, ions can be made into an ion containing solution, and can be easily contacted with the application site | part (cell) by using a cannula, a syringe, etc., for example.

Hereinafter, the content of the second invention group will be specifically described based on the experimental example (experimental example as the second invention group example).

(Example 1)

Example 1 relates to a cell activity control method according to the invention of the second invention group.

(First experiment)

Silver ions were prepared as ions for controlling cell activity. Silver is characterized by a small ionization tendency, difficult to emit electrons, difficult to oxidize, and a standard monopolar potential of +0.8 V, which is difficult to become a cation. For this reason, even if silver in a metal state is not easily eluted, in Example 1, the method of applying an electric field to silver was used as a preparation method of a silver ion containing solution. Specifically, as shown in FIG. 10, two pure silver plates 202 are immersed in a container 201 in which the solution 203 is placed, and a DC current 222 of 10 to 50 mA flows between both plates. To 50V or less. As a result, silver ions were eluted into the solution to prepare a silver ion-containing solution 203.

As the solution, a phosphate buffer [PBS (-)] consisting of 8.0 g / L NaCl, 0.2 g / L KCl, 1.15 g / L Na ₂ HPO ₄ , 0.2 g / L Na ₂ PO ₄ and water was used. It was. In addition, the glass beaker was used as the container 201 (FIG. 10) which produces a silver ion containing solution. In addition, since the material of the container 201 is preferably inert to the silver ion-containing solution, it is preferable to use a container made of resin such as glass, acrylic, Teflon (registered trademark), or stainless steel.

According to the method, for example, by energizing for 60 seconds at 12.5 kV, a silver ion solution of about 1 ppm can be produced.

In addition, a silver ion containing solution can also be produced using silver ion zeolite. Specifically, a 100 ppm silver ion-containing solution can be prepared by adding 1 g of silver ion zeolite (NFG Ag ion zeolite) manufactured by Cinnanen Zeomik Co., Ltd. or N.F.G Co., Ltd. in 10 mL of pure water.

Next, human epithelial-derived squamous epithelial cells (malignant tumor cells) were prepared as cells to be applied. The cells (2 × 10 ⁵ cells) were placed in the bottom surface of a 90 mm chalet (polystyrene chalet made by IWAKI), and an appropriate amount of culture solution was added thereto. As the culture medium, a culture medium prepared by mixing 1% L-glutamine, 10% sodium bicarbonate, 10% fetal bovine serum, and an appropriate amount of phenol red in Eagle MEM medium (made by Nissui) was used. In this manner, two test chalets C1 and D1 containing malignant tumor cells were prepared.

The test chalet C1 · D1 was incubated for 2-3 days in a CO ₂ incubator until 8 × 10 ⁵ tumor cells proliferated on the bottom of the chalet. Thereafter, the culture medium in the chalet was removed with an aspirator and the cells were rinsed in 10 mL of Dulbecco's phosphate buffer / phosphate buffer (-). Rinse is an operation for removing the influence by the culture liquid and determining the action of silver ions only.

In addition, the phosphate buffer (-) is a solution consisting of NaCl of 8.0 g / L, KCl of 0.2 g / L, Na ₂ HPO ₄ of 1.15 g / L, Na ₂ PO ₄ of 0.2 g / L and water in the same manner as above. to be.

As shown in FIG. 11, a test chalet C1 (FIG. 11 code 205) and D1 (FIG. 11 code 206) were put in a dark box 209, and a silver ion containing solution having a concentration of 50 ppm of silver ions diluted in phosphate buffer (-). 6 mL was injected into the cell group (204 * 204) in a test chalet by syringe. After pouring, the test chalet D1 (FIG. 11 code 206) was irradiated with light having a wavelength of 365 nm at an irradiation intensity of 2 mW / cm 2 for 30 minutes. On the other hand, the test chalet C (FIG. 11 code 205) was used as a reference for judging the light irradiation effect without performing light irradiation.

In the light irradiation, Nichia Chemical Co., Ltd. ultraviolet light emitting chip type LEDNCSU033A (T) was used as a light source, and a fluorine-containing silica glass (modified silica) was used as the light lead from the light source to the cell surface. The deep ultraviolet optical fiber (DUV-S) made from Densen Kabushiki Kaisha was used. The optical fiber in which a trace amount of fluorine is added to silica glass is preferable because the transmittance | permeability in an ultraviolet region improves and irradiation resistance improves. Reference numeral 208 in Fig. 11 represents silver ions in contact with the cells.

In addition, two test chalets A1 and B1 were produced in the same manner as above except for using a phosphate buffer (-) containing no silver ions. This was put into the dark box 9 similarly to the above, and was irradiated with the light of wavelength 365nm only to chalet B1 for 30 minutes by irradiation intensity of 2 mW / cm <2>.

The solution was aspirated and removed from each test chalet described above, and each test chalet was then rinsed three times using 10 mL of phosphate buffer (-). After rinsing, 4 mL of phosphate buffer (-) was added dropwise to each test chalet.

The test chalets A1 to D1 subjected to the above operation were examined by the fluorescent staining method using the Cellstain-Double Stainingi Kit manufactured by Tojin Kagaku Co., Ltd., to investigate the degree of proliferation impairment of tumor cells.

First, the fluorescence staining method will be described. In the cell staining cell double staining kit, Calcein-AM and Propidium Iodide function as follows. Calcein-AM functions as a fluorescent dye for live cell staining. Calcein-AM has four carboxyl groups of Calcein acetoxymethylated to increase fat solubility. Therefore, cell membrane permeability is high. Calcein-AM itself does not fluoresce, but when it enters a living cell, the acetoxy methyl group is hydrolyzed by the esterase in the cell to emit yellowish green fluorescence. On the other hand, since dead cells have no activity in esterases, Calcein-AM does not hydrolyze and thereby does not fluoresce.

In the CellStain Cell Double Staining Kit, Propidium Iodide (PI) functions as a fluorescent dye for dead cell staining. Propidium Iodide is introduced into cells only when the cell membrane is destroyed, intercalates into a double helix structure of DNA, and emits red fluorescence. Propidium Iodide, on the other hand, cannot penetrate the cell membranes of living cells, and thus does not fluoresce in living cells. Thus, using the CellStain Cell Double Staining Kit, when observed at a wavelength of 400-440 nm through a band pass filter, live cells and dead cells can be observed simultaneously.

In this experiment, 20 μL of Calcein-AM stock solution (1 mmol / L) as solution A and 30 μL of PI stock solution (1.5 mmol / L) as solution B were mixed and used in 10 ml of phosphate buffer (−). Calcein-AM concentration in this mixed solution is 2 μmol / L, and PI concentration is 4 μmol / L.

2 mL of this mixed solution was dripped in each test chalet, and it incubated for 15 minutes in 37 degreeC thermostat. Thereafter, a filter of 490 ± 10 nm was used to count the number of cells (live cells) stained in yellow green and the number of cells (dead cells) stained in red.

The measurement results were listed in Table 201. In addition, the evaluation by the criterion of the human leukocyte antigen lymphocyte cell disorder test method shown in Table 202 was also described in Table 201.

From the results in Table 201, it was recognized that the application of both silver ions and light to tumor cells significantly increased the dead cell rate by 5.5 times compared to silver ions only (no light irradiation). Moreover, it was confirmed that this effect is not the death of the cell by light.

Specifically, it was evident from the comparison of the results of the test chalet A1 and the test chalet B1 that only the specific light (ultraviolet light) of the irradiation intensity used in the above experiment did not substantially hinder the growth of the tumor cells. In addition, from the comparison of the results of the test chalets B1 and C1, it became clear that the difference between them is due to the application of silver ions.

Based on these results, the results of the test chalets A1, B1, C1, and D1 are compared, and the significant increase in the dead cell rate in the test chalet D1 is due to the effect of inhibiting the growth of ultraviolet light itself and the effect of inhibiting the growth of silver ions themselves. It becomes clear that it is not simply added.

As mentioned above, the remarkable increase of the dead cell rate in the test chalet D1 is due to the introduction of metal ions into the cells by specific light (in this case, ultraviolet light), and more silver ions are introduced into the cells. It can be considered that this is a result of remarkably exerting the effect of growth and growth on the living body.

To verify this consideration, various samples obtained by differentiating nuclei, mitochondria, intranuclear proteins, and cytosol from tumor cells A1 to D1 treated under the same conditions as in Table 201 were prepared, and the radiation of the large radiation facility (Spring-8) was produced. Light was irradiated to each sample and the fluorescent X-ray was measured. Here, for the differentiation of the nucleus and the mitochondria, a mitochondrial extraction kit manufactured by Pierce Corporation was used, and a nuclear protein extraction kit (ReadyPrep Cytoplasmic / Nuclear) manufactured by BioRed Corporation was used for the extraction of nuclear proteins.

In the analysis, the characteristic X-rays emitted from the sample were provided with a Si (Li) semiconductor detector using a beamline BL37XU (hard X-ray region unculator beamline, excitation X-ray 30 keV, slit: length 0.2 mm x width 0.5 mm). It was measured by one fluorescence X-ray analyzer.

As a result of the measurement, in the comparison of the cell which applied both silver ion and light irradiation of wavelength 365nm, the cell which applied only silver ion, the cell which applied only light, and the cell which none apply, the silver ion and wavelength 365nm The cells to which both light irradiations were applied were found to have a large amount of silver introduced into organella such as cell nuclei and mitochondria compared with other cells. It was also recognized that silver was introduced more into the cell nucleus.

On the other hand, the amount of silver in the cytosol was small, and the increase of the introduction amount by light irradiation was not recognized about the cytosol. For this reason, in order to perform a more detailed analysis about the introduction of silver ions into the cell nucleus, the protein in the nucleus was extracted from the cell nucleus, separated from other components such as DNA, and the presence of silver in the nucleus protein was examined. As a result, it was recognized that the amount of silver in the nucleus protein tended to decrease rather.

Compared with this result and the case where the light is not irradiated, the fact that the amount of silver introduced into the nucleus increases in the cells which are irradiated with light, the silver introduced into the cell is combined with the nucleic acid component in the cell nucleus. I think.

As described above, according to the present invention in which both silver ions and specific light are applied to living cells, it can be demonstrated that silver can be remarkably introduced into living cells. Moreover, such an effect could demonstrate that sufficient light (specific light) does not prevent the growth or proliferation of living cells.

(2nd experiment)

In place of the epithelial cells (malignant tumor cells) derived from the laryngeal cancer, small intestinal intestinal mucosa cells (normal cells) were used, and in addition to this, test chalets A2 to D2 were produced in the same manner as in the first experiment. The influence of specific light was investigated. The results are shown in Table 203.

As apparent from the results of Table 203, it was recognized that even in the small intestine mucosal cells (normal cells), which are not malignant tumor cells, a large dead cell rate was increased by combination of silver ions and specific light irradiation. However, as apparent from the comparison between Table 201 and Table 203, the effect of increasing the dead cell rate (i.e., inhibiting cell proliferation) by specific light irradiation is markedly different in the case of malignant tumor cells and normal cells. It was recognized that the side of malignant tumor cells was remarkably large.

From this, when the method of the present invention is applied to, for example, a cancer patient, the effect is strong because it is strongly developed in cancer cells and weak in normal cells. In addition, the method of the present invention uses specific light, but since light is good in directivity, the light can be irradiated only to the affected area, and at the same time, it is weak to normal cells because specific light is sufficient as weak light that does not damage cells. In addition to its properties, it is possible to kill only cancer cells without damaging normal cells.

(3rd experiment)

In about half of one chalet, squamous cell-derived squamous epithelial cells (malignant tumor cells) are placed, and intestine intestinal mucosa cells (normal cells) are put in about half of the other side, and cultured under the same conditions. The test chalets A3 to D3 were produced in the same manner as in the first experiment. For these test chalets A3 to D3, viable cells stained with yellow green and dead cells stained with red were observed in the same manner as in the first experiment.

 As a result, in test chalet D3 to which both silver ions and specific light were applied, a very clear difference was recognized between one half face and the other half face of the test chalet. In other words, half of the cultured larynx cancer-derived squamous epithelial cells was significantly red, and half of the intestinal intestinal mucosal cells was yellow-green. This result is coded as a result of the first and second experiments, and it is also confirmed that the amount of silver ion introduced into the malignant tumor is increased beyond normal cells by irradiation of specific light that does not damage the cells. It became.

(4th experiment)

Test chalets E (25 ppm), F (25 ppm), G (10 ppm), and H (10 ppm) were produced in the same manner as in the first experiment except that the silver ion concentration was set to 10 ppm and 25 ppm. Using these test chalets, cell proliferation activity was examined in the same manner as in the first embodiment. The results are listed in Table 204 together with the results in Table 201.

From the comparison of the results of C1, E, and G of Table 204, when no specific light is irradiated, as the ion concentration increases in the order of G (10 ppm)-> E (25 ppm)-> C1 (50 ppm), the dead cell rate Although the tendency to become large at this 14.6%, 16.6%, and 17.9% was recognized, the difference was very small. On the other hand, it was recognized from the comparison of the results of D1, F, and H that the dead cell rate was remarkably increased only at 50 ppm compared with both 10 ppm and 25 ppm when irradiating specific light.

This result means that although the control effect of cell activity has a concentration dependency on silver ions, the relationship with cell death is not linear. Therefore, it is necessary to appropriately select the metal ion concentration in accordance with the purpose of cell activity control. The reason why the relationship between the silver ion concentration and the cell death is not linear is that although the introduction of silver ions into the cell is accelerated by the irradiation of specific light, if the silver ion concentration in the external world is too low, it is introduced into the cell within a certain time. Since the absolute amount of silver ions becomes small, it can be inferred that it may not cause cell death.

(Experiment 5)

As a specific light, the wavelength of 365 nm, 400 nm, 525 nm, 600 nm, 660 nm is used, and the type of light and the laryngeal cancer-derived squamous epithelial cell on condition that light intensity is 2 ㎽ / ㎠ The relationship of silver ion introduction amount was investigated by the method similar to the said 1st experiment. The results are shown in Table 205. In addition, the test was conducted again with a wavelength of 365 nm separately from the first experiment.

From Table 205, the dead cell rate was changed by the wavelength of the light to be irradiated, and in particular, a large variation was observed between the wavelengths of 600 nm and 660 nm, and the dead cell rate fell from 94% to 18%.

From this, it turned out that the ion permeation channel of a cell membrane can be opened effectively by applying the light of a specific wavelength range. In addition, from the tendency that can be read in Table 205, an effect of inhibiting cell activity is obtained even at a wavelength of less than 365 nm. However, since wavelength light of less than 300 nm itself damages the DNA of normal cells, it is not suitable as light which opens the ion permeation channel of living cells. Therefore, as specific light, 300 nm or more and 660 nm are preferable, More preferably, 300 nm or more, 600 nm or 300 nm or more, 525 nm, More preferably, 365 nm or more and 525 nm are preferable.

(Example 2)

Example 2 relates to a cell activity control device for applying the cell activity control method described in Example 1. 12 is a cell activity control device according to the present invention. 12, the outline | summary of the cell activity control apparatus which concerns on Example 2 is demonstrated.

This apparatus is connected to the metal ion generating container 211, the metal plate 212, the ion-light carrier tube 214, the syringe unit 215, and the second control unit 221 connected to the first control unit 220. The light source unit 216, the application target unit (cell) 217, the fluorescence detection unit 218, and the computer 219 are provided. The first control unit 220 is a device for applying a voltage to the metal ion generating container 211 by appropriately controlling the necessary conditions such as the voltage application amount, the application time, etc. according to the program stored in the computer 219, the second control unit 221 is an apparatus that controls the light source unit 216 to emit specific light under appropriate conditions (light irradiation intensity, irradiation time, etc.) in accordance with a program stored in the computer 219.

The metal plate 212 is disposed inside the metal ion generating container 211, and a direct current applied voltage 213 is applied to the metal plate 212. In addition, a solution injection port (not shown) through which the solution can be injected is formed in the metal ion generating container 211, and the solution can be injected as needed.

At least one metal play of the metal ion generating container 211 is a silver plate, a phosphate buffer (-) described below is injected from the solution inlet, and then a voltage is applied to the metal plate 212. As a result, a metal ion (silver ion) -containing solution in which silver ions elute in the phosphate buffer is prepared.

The ion-light carrier tube 214 consists of a cavity part and an optical fiber part surrounding this cavity part, the cavity part of an axial center side functions as a flow path through which an ion solution passes, and the optical fiber part functions as an optical transmission path. The syringe portion 215 quantitatively delivers a metal ion solution to the cavity. The syringe portion 215 is disposed between the metal ion generating container 211 and the ion-light carrier tube 214, and in the example of the apparatus of FIG. 3, an optical fiber member capable of transmitting light to the outside of the syringe portion 215. Is arranged.

The light source unit 216 has a light source capable of generating light (specific light) having a peak wavelength in the range of 300 to 600 nm and having an irradiation intensity of 500 to 500,000 mW / cm 2. The tip of is connected to the optical fiber member outside the syringe portion 215. Therefore, the specific light generated by the light source portion is guided to the application target portion (cell) 217 through the fiber member of the syringe portion 215 and the fiber portion of the ion-light carrier tube 214. In addition, the syringe part 215 is not essential, and the light source part 216 may be directly connected to the optical fiber part of the ion-light carrier tube 214 without interposing the syringe part 215.

As the light source, for example, an ultraviolet light emitting chip type LEDNCSU033A (T) manufactured by Nichia Kagaku Kogyo Co., Ltd. may be used, and the LEDs are fixed to at least one aluminum substrate having a thickness of 1 mm. It can be set as the structure which has a heat sink. Moreover, it is good also as a structure which can vary an optical output.

The application target portion 217 is a region where cells to be subjected to the cell activity control method are present. In the example of FIG. 12, the cell group put in the chalet becomes the application target portion 217. In practical application, it becomes the affected part of the patient which a tumor developed, for example.

The fluorescence detection unit 218 is an apparatus for optically reading the state of the cells in the application target unit to which the cell activity control method is applied, and has a light sensor that irradiates fluorescence and detects the reflected light. The information detected by the optical sensor of the fluorescence detection unit 218 is output to the computer 219, and the computer 219 processes the information based on a preset image processing program to proliferate the cells present in the adaptation target unit. The activity level is determined and displayed, for example, on the display of the computer 219. As the fluorescent sensor, for example, a charge coupled device (CCD) can be used.

In addition, a solution injection device for quantitatively or continuously injecting a solution may be connected to the injection port of the metal ion generating container 211. In this case, the syringe portion 215 for quantitatively or continuously feeding the metal ion solution is provided. You do not have to.

In the cell activity control apparatus described above, the optical fiber portion of the ion-light carrier tube 214 serves as an irradiation portion for inducing the output light of the light source portion to the animal cells and irradiating the light to the animal cells. Further, the tip portion of the cavity portion of the ion-light carrier tube 214 serves as a contact portion for bringing metal ions into contact with the cells. That is, in the above apparatus, the ion-light carrier tube 214 serves as a contact portion and an irradiation portion.

[Third invention group]

EMBODIMENT OF THE INVENTION Hereinafter, the form for implementing 3rd invention group is demonstrated based on an Example. In addition, the Example described below is an illustration and is not restrictive.

(Example 1)

The refrigerator provided with the automatic ice-making apparatus of Example 1 is a refrigerator compartment (FIG. 1 refrigerator compartment ⇒) controlled about 1 degreeC-6 degreeC, and the freezer compartment controlled by temperature below 0 degreeC (generally -18 degrees C or less) (FIG. It is a refrigerator provided with at least 1 freezer compartment ⇒), which is further equipped with an automatic ice maker. Elements other than the automatic ice-making apparatus of this refrigerator may be comprised based on a well-known technique, and there is no restriction | limiting in particular. Therefore, the automatic ice-making apparatus which is a main part of this refrigerator is demonstrated here, referring FIG.

It is a conceptual perspective view for demonstrating the whole automatic ice-making apparatus. This automatic ice making apparatus includes a water supply tank 301 that can be removed, a water pipe 302, an ice tray 303, a storage container 304, a heat insulating member 305, a silver ion supply unit 306, and a silver ion supply unit 306. ), An electromagnetic valve 307 that opens and closes the flow of silver ions injected into the water pipe 302, a light source A 308, a liquid feeding pump 314, and an electromagnetic valve 315.

The water supply tank 301 and a part of the water pipe 302 are arranged in the refrigerating chamber, the other part of the water pipe 302, the ice making dish 303, and the storage container are arranged in one section of the freezer compartment, and the cold compartment and the freezer compartment are insulated. It is partitioned by the member 305.

In addition, although not shown in figure, the ice tray 303 can rotate by a rotating motor, and the heat plate is arrange | positioned at the bottom surface of an ice tray.

In addition, an electromagnetic valve 315 is provided between the water supply tank 301 and the liquid feed pump 314 to open and close the flow of water from the water supply tank 301 to the water pipe 302. Is controlled to transfer a certain amount of water every fixed time.

The operation of the rotation of the ice tray 303, the ON of the heat plate, the OFF of the heat plate, the ON / OFF of each electromagnetic valve, the driving of the liquid feed pump are all microcomputer controlled (not shown). For example, a height sensor is provided in the storage container, and when the storage amount reaches a constant height, the height sensor issues a signal for limiting the driving of the liquid feeding pump to the microcomputer.

The said silver ion supply part 306 is comprised from the tank made from Teflon (trademark), and the silver ion aqueous solution of 60 ppb density | concentration is accommodated.

The water pipe 302 is comprised from transparent materials, such as transparent resin and transparent glass.

The light source A is light which has a peak wavelength in the range of 300 nm or more and 600 nm or less, and is a light source from which the irradiation intensity of 500-500,000 mW / cm <2> is obtained. In this embodiment, using an LED having a peak wavelength at 525 nm, the irradiation intensity is set to 50,000 mW / cm (50 mW / cm 2).

In addition, although FIG. 13 makes one light source, since the light source A is not limited to one, it arrange | positions in multiple numbers as needed. In addition, the light source A may be a fluorescent lamp.

In the automatic ice making device of this structure, the peak wavelength, the light irradiation intensity and the irradiation time of the light source A may be appropriately selected in consideration of the light transmission characteristics of the water pipe, the silver ion concentration, the required antibacterial power, and the like. When a peak wavelength light source that is difficult to penetrate the water pipe wall is selected, since light does not sufficiently reach the inside of the water pipe, the antibacterial effect enhancement effect due to light irradiation is not obtained, and the silver ion concentration is low and the light irradiation intensity is small. If the irradiation time is shortened, the antimicrobial effect is insufficient. Therefore, irradiation time is made into 30 minutes or more normally, and is set to 60 minutes in Example 1.

The reflecting plates 309 · 310, 311 are members for sufficiently light to reach the entire water pipe 302. The reflecting plate is composed of a material capable of reflecting light, for example a mirror plate made of aluminum.

Although the enlarged conceptual diagram of a reflecting plate is shown in FIG. 14, a reflecting plate is not essential. In the case where the light can be sufficiently brought into the entire water pipe 302 with only the light source, a reflecting plate may not be provided. In addition, in the 3rd invention group, all the elements for irradiating light to a water pipe, including a light source, a reflecting plate, etc. are called a light source part.

The ice making operation of the ice making apparatus will be described.

When the water supply tank 301 containing water is installed in the refrigerating chamber, the installation of the water supply tank is detected by a sensor (any sensor) provided in the mounting portion of the water supply tank 301. As a result, the electromagnetic valve 315 is opened, and the liquid feed pump 314 is driven so that a certain amount of water in the water supply tank 301 is fed to the ice making tray 303. At this time, the electromagnetic valve 307 is closed.

The water stored in the ice tray 303 is cooled down (for a predetermined time period) until it is sufficiently frozen. Thereafter, the ice making tray 303 is rotated half by a rotation motor (not shown), and the heat plate is turned on in the state where the bottom surface is upward, and the bottom surface is heated. As a result, the contact surface with the ice making tray melts and the ice in the ice making tray 303 falls in the storage container 304, and is stored.

Thereafter, the ice making tray 303 is again rotated by the rotating motor to return to its original state, and the liquid feeding is started again in synchronization with this.

These series of operations are integratedly controlled by a microcomputer (not shown).

In addition, although the method which arrange | positioned the heat plate at the bottom surface of an ice tray and rotates an ice tray by 180 degree | times in the step in which the water in an ice tray was cooled, about the storage and take-out means of ice making ice is specifically limited Since there is no known method can be used.

Next, the germicidal operation (also called a purifying operation) of the water pipe 302 will be described.

First, the electromagnetic valve 315 is closed and the electromagnetic valve 307 is opened. In this state, a certain amount of the 60-ppb concentration silver ion aqueous solution stored in the silver ion supply part 306 arrange | positioned in the refrigerating chamber is injected into the water pipe 302 by the liquid feed pump 314. FIG. As said fixed amount, it is set as the liquid amount which the water pipe 302 fills with silver aqueous solution.

This operation is performed at a predetermined time interval (for example, once a week) or based on an arbitrary operation instruction of the user, and in the state where a certain amount of silver ions flows into the water pipe 302, the electromagnetic valve ( 307 is closed.

On the other hand, the light source A is turned on after the start of the operation of pouring the silver ion aqueous solution into the water pipe 302 (about 30 to 10 minutes before) or after the silver ion aqueous solution is filled in the water pipe 302. Light is irradiated to the inner wall of 302. Light irradiation time is set to 60 minutes.

The pouring operation of the said silver ion aqueous solution, the timing of light irradiation, and the lighting time (light irradiation time) of the light source A are all controlled by the microcomputer.

After the injection of the silver ion aqueous solution into the water pipe 302 and the antibacterial action by light irradiation are finished, the silver ion removal operation is performed if necessary.

Although silver ion removal operation | movement is an operation | movement which wash | cleans silver ion which exists in a water pipe etc., since the bypass path is not provided in the automatic ice-making apparatus of Example 1, the water (clean water) which flowed through the water pipe 302 is The deicing dish 303 is stored. Therefore, in the silver ion removal operation in this embodiment, the water in the water supply tank flows to the ice making tray 303, and the water or ice in the ice making tray is discarded. However, in the present embodiment, since an extremely low concentration of silver ion aqueous solution of 60 ppb concentration is used, there is no particular problem even if the silver ion washing step is not performed.

(Example 2)

15 is a conceptual perspective view of the ice making apparatus according to the second embodiment. In Embodiment 2, a three-way branch path 316 with an electromagnetic valve is provided between the water supply pipe 302 and an opening and closing of each flow path between the water supply tank 301 and the liquid feed pump 314. The same three-way branch path 317 with an electromagnetic valve is provided at the point where the silver ion supply portion 306 is connected to the flow path, and in the middle of the water pipe 302 and near the end of the refrigerator compartment side. The light guide layer-coated water pipe 319 coated with the light pipe 302 with a light guide layer made of a light guide material (optical fiber) instead of a reflection plate, and a silver ion supply unit instead of the reflection plate. It is characterized by storing an aqueous silver ion solution having a concentration of 1100 ppb at 306.

About matters other than this, it is the same as that of the said Example 1.

Explanatory drawing of the light guide layer coating water pipe 319 is shown in FIG. FIG. 16A is a light guide layer-coated water pipe 319, and FIG. 16B is a sectional view taken along line X-Y in FIG. Reference numeral 302 in FIG. 16B denotes a water pipe, and reference numeral 320 denotes a light guide layer covering the water pipe. Although not shown, light is injected into the light guide layer 320 from the outside through an optical fiber. When this light guide layer coating water pipe 319 is used, light can be irradiated to the water pipe 302 easily.

The automatic ice making apparatus according to the second embodiment of this structure operates as follows.

In normal operation, the electromagnetic valve and the liquid feed pump operate so that water is supplied from the water supply tank 301 to the ice making tray 303, and ice making is performed. On the other hand, the antibacterial treatment operation is performed at a predetermined timing, for example, about once a month.

The antibacterial treatment operation operates the valve of the three-way branch path 316 to shut off the flow of water from the water supply tank 301, and operates the valve of the three-way branch path 317 to control the flow of water to the ice making tray 303. At the same time, water flows from the three-way branch 317 to the drain 318.

Subsequently, the three-way branch path 316 is operated so that the silver ion aqueous solution in the silver ion supply part 306 flows in the light guide layer covering water pipe 319, and the liquid supply pump 314 covers the silver ion aqueous solution with the light guide layer. The liquid is fed into the water pipe 319.

On the other hand, in synchronization with the liquid supply of the silver ion aqueous solution, the light in which irradiation intensity light of peak wavelength 525nm and 50 kHz / cm <2> is obtained is introduce | transduced into the light guide layer of the light guide layer coating water pipe 319 for 30 minutes. As a result, the microorganisms adhered to or propagated on the inner wall to which the inner wall of the water pipe is irradiated are antibacterial.

Here, although the silver ion aqueous solution may flow in the drainage path 318, in the stage where the water pipe 302 replaced with the silver ion aqueous solution, the feed pump 314 was stopped and all the flow paths of the three-way branching paths 316 * 317 are closed. In this state, light irradiation for 30 minutes may be performed. In this way, the usage-amount of a silver ion aqueous solution can be reduced.

Following the antimicrobial treatment operation, the flow path of the three-way branch paths 316 and 317 is switched to form a flow of the water supply tank 301-the water pipe 302-the drain path 318, and the water supply tank 301 Pour water over a period of time (for example, 5 minutes). By this silver ion removal operation, silver ions in the water pipe 302 are extruded to the drainage path, and silver ions in the water pipe are removed.

In addition, it is preferable that the drain passage 318 has a structure in which the washing liquid can be taken out of the refrigerator.

After the antibacterial treatment operation and the silver ion removal operation are completed, the respective electromagnetic valves are operated to perform normal deicing.

Here, although the silver ion aqueous solution of 1100 ppb density | concentration was accommodated in the silver ion supply part 306 above, the water supply from the water supply tank 301 is stored in the silver ion supply part 306 with higher concentration of silver ion aqueous solution. By mixing with, the concentration of silver ions in the water pipe 302 may be around 1100 ppb.

(Example 3)

17 is a conceptual perspective view of the ice making apparatus according to the third embodiment. In Example 3, light irradiation was also possible to the water supply tank 301, and the silver ion supply part (including the electromagnetic valve 307) was connected to the water supply tank 301. In addition, the silver ion supply part stored the silver ion aqueous solution of 60 ppb concentration. About the matters other than this, it was similar to the said Example 2.

In the ice making apparatus which concerns on a present Example, since silver ions are contained in the water in a water supply tank, and light irradiation is also made to the water supply tank, the antibacterial of a water supply tank can be estimated.

In the automatic ice making apparatus of this structure, instead of the silver ion aqueous solution having a concentration of 60 ppb, for example, a high concentration of silver ion aqueous solution of 2000 ppb is stored in the silver ion supply unit, and the silver ion concentration in the water supply tank is 60 ppb (or It is good to set it as the structure which adjusts supply of silver ion aqueous solution so that it may become the following). In this way, there is an advantage that the silver ion supply portion can be made small.

(Common matters)

As the silver ion supply unit of the first to third embodiments, a cartridge filled with silver ions can be employed. For example, when the disposable type cartridge in which a predetermined amount of silver ion aqueous solution is accommodated is used, the amount of silver ions supplied to the inside of the water pipe can be controlled easily.

In addition, instead of accommodating the silver ion aqueous solution in the silver ion supply unit 306, a method of generating silver ions by receiving two silver plates in the silver ion supply unit 306 (or a water supply tank) to apply a DC current. Can be adopted.

Moreover, the silver ion containing zeolite etc. are accommodated in the silver ion supply part 306, and the method of extracting from water at the time of use can also be employ | adopted.

In addition, a silver ion eluent such as a silver ion-containing zeolite or a silver ion eluting glass is disposed in the water supply tank or in the water pipe, instead of newly installing the silver ion supply unit, and the silver ion is eluted when the zeolite contacts water. You may make it.

Moreover, you may mix | blend silver ion eluant, etc. with the material which comprises a feed water tank and / or a water pipe, an ice making dish, and / or an ice making compartment. In such a silver ion eluting material, since silver ion concentration after silver ion eluting cannot be made very high, safety is high. At the same time, being unable to make a high concentration means that a high effect cannot be obtained. However, as in the present invention, the effect can be enhanced by irradiating light, and a high effect can be obtained even at a low silver ion concentration.

Moreover, in the case of the structure which arrange | positions a silver plate in the water supply tank 301, the silver plate and application means provided in the water supply tank 301 correspond to the silver ion supply part 306. As shown in FIG.

(Antibacterial effect confirmation experiment 1)

In the antibacterial process described in Example 1, it was confirmed by the following experiment that the inside of the water pipe was antibacterial. The experimental method was as follows.

(i) As an antimicrobial target, Candida albicans NBRC-1060 standard strain (eukaryote) of about 5 × 10 ⁷ cells / mL was prepared. The cells were dispersed in a phosphate buffer (concentration 50 mM) to prepare a spore suspension (bacteria solution). As a container for preparing spore suspension, a glass container was used.

(ii) 1 mL of the said spore suspension was disperse | distributed to 500 mL of water, and the microbial contaminant water of 1 * 10 <^5> / mL was prepared. This was put into the water supply tank 301.

The ice making device is set to the flow of the water supply tank 301 to the water pipe 302 to the ice tray 303, and the delivery pump 314 is driven to flow the microorganism contaminated water into the water pipe 302. After 30 minutes, 60 minutes later, microbial contaminated water in the ice making dish 303 was collected (test solution; contaminated water).

(iii) In addition, using contaminated water similar to ii above, the condensed water was filled in the water pipe 302 with light having a peak wavelength of 525 nm at a radiation intensity of 50 mW / cm 2 for 15 minutes, 30 minutes, and 60 minutes. The test solution in the case of irradiating for a minute was collected from the ice making dish 303 (test solution; contaminated water + light).

(iv) In addition, 1 mL of the spore suspension and 50 mL of silver ion aqueous solution having a silver ion concentration of 600 ppb were added to 450 mL of water to prepare microbial contaminated water (1 × 10 ⁵ cells / mL) having a silver ion concentration of 60 ppb. In the same manner as in ii, the contaminated water was collected from the ice making tray 303 (test solution; contaminated water + silver ions).

(v) In addition, antibacterial contaminated water irradiated with light having a peak wavelength of 525 nm at a radiation intensity of 50 mW / cm 2 for 15 minutes, 30 minutes, and 60 minutes using silver ion-containing contaminated water such as iii. Were respectively collected from the ice making dish 303 (test solution; contaminated water + silver ions + light).

(Antibacterial effect confirmation experiment 2)

As a microorganism to be antimicrobial, a test solution using Escherichia coli was collected in the same manner as in Experiment 1 except that Escherichia coli (NBRC-3972, Escherichia coli) was used instead of Candida albicans NBRC-1060.

100 μL of each test solution collected above was plated on a potato agar medium, incubated at 25 ° C. for 4 days, and then the antimicrobial effect was examined by a method of counting the number of colonies on the medium.

(result)

The result in the experiment 1 is shown in FIG. 18, and the result in the experiment 2 is shown in FIG.

18, the bactericidal effect was not recognized only by light irradiation from the comparison between only contaminated water containing Candida albicans (▲ in the figure) and when irradiated with contaminated water (Δ in the figure).

On the other hand, from the comparison of an antimicrobial method containing silver ions but not light irradiation (● in the drawing) and an antimicrobial method (o in the drawing) that contains silver ions and light irradiation, the remaining number of Candida albicans was 1 digit by light irradiation. Significant bactericidal potency effect that was lowered abnormally was recognized.

In addition, as apparent from FIG. 19, the same result was obtained for E. coli.

18 and 19, it was recognized that the longer the light irradiation time is, the more the difference with the antibacterial effect in the case of containing silver ions but not light irradiation (in the figure) was enlarged.

From these results, the following can be concluded.

The irradiation intensity of 50 mA / cm 2 of the ultraviolet light (525 nm) used in the above experiment is sufficiently lower than the strength capable of antibacterial fungi. Therefore, the difference between the antimicrobial method containing silver ions and light irradiation (○ in the figure) and the antimicrobial power when silver ion is included but not light irradiation (● in the figure) is due to the sterilizing effect of ultraviolet light itself. Rather, it is considered that the ultraviolet light irradiation accelerates the introduction of silver ions into the cells. That is, it is thought that the antimicrobial effect of silver ions increased as a result of the introduction of many silver ions into the cells of Candida albicans or E. coli by irradiation with light having a peak wavelength of 525 nm and irradiation intensity of 50 mA / cm 2.

In addition, although silver ion was used as antimicrobial ion in the said Example, application of this invention is not limited to silver ion. For example, copper ions, zinc ions, or the like may be used.

Moreover, although the example which supplies water from the water supply tank was shown, the form which supplies from a water supply through a valve etc. may be sufficient. In this case, since there is no tank, microorganisms do not grow in the tank, but there is a fear that microorganisms grow in the water pipe.

[4th invention group]

EMBODIMENT OF THE INVENTION Hereinafter, the form for implementing 4th invention group is demonstrated based on an Example. In addition, the Example described below is an illustration and is not restrictive.

(Example 1)

The rotating drum type washing machine provided with the antibacterial treatment function which concerns on Example 1 is demonstrated, referring FIG. It is a cross-sectional schematic diagram for demonstrating the inside of a washing machine.

The rotary drum type washing machine 400 includes at least an outer frame 401, a water tank 402, a rotating drum 403, a motor 404 for rotating the rotating drum, a washing machine door 405, a water supply pipe 406, and a drain pipe. 407, the silver ion supply unit 409 which comprises silver ion supply means, and the light source 410 as a 1st irradiation part are provided.

The water supply pipe 406 is a pipe for introducing water into the washing machine, and the water tank 402 receives tap water for washing or rinsing through the water supply pipe 406, and discharges their water to the outside of the washing machine through the drain pipe 407. It is made to structure.

In front of the water tank 402, an opening for entering and exiting laundry is formed, and the opening is provided with a washing machine door 405, and the water tank can be opened and closed.

The rotary drum 403 is disposed in the water tank 402, and the motor 404 is connected to the bottom surface, and is able to rotate forward and backward in the water tank 402. The rotary drum 403 has an opening in front of the laundry. The baffle 412 for rolling the laundry in the rotational direction at the time of rotation is provided on the inner side (inner circumferential surface) of the rotary drum.

In addition, a plurality of holes 408 are formed in the side surface (peripheral surface) of the rotary drum 403, and the holes 408 are centrifugal at the time of washing and rinsing the water inside the rotating drum and the water tank during washing. When dehydrated, it functions to discharge water or warm air in the rotating drum to the outside of the drum upon drying.

The silver ion supply unit 409 is provided in the middle of the water supply pipe 406, and generates and supplies silver ions in water (usually tap water). The generated silver ions are supplied into the rotating drum through the water supply pipe 406.

 The silver ion supply unit 409 consists of two silver plates and a power supply which applies a DC voltage to this, and the silver ion concentration of the silver ion water produced by the silver ion supply unit 409 applies to a silver plate. It is controlled by varying the direct current voltage and / or by varying the supply amount of water, and microcomputer controlled so that the required silver ion concentration is supplied into the rotating drum as needed.

The silver ion concentration of the silver ion water supplied into the rotating drum is within the range of 1 ppb to 10 ppm, preferably 5 ppb to 1100 ppb, more preferably 10 ppb to 900 ppb, still more preferably 30 ppb to 100 ppb, or 30 ppb to 60 ppb. do. The higher the silver ion concentration, the stronger the antimicrobial activity, and the lower the silver ion, the more friendly the natural environment is. In addition, when supplying silver ion water of the concentration exceeding 2000 ppb, the zeolite, ceramics, etc. which carry silver ion are arrange | positioned in a silver ion supply unit.

The light source 410 as the first irradiation unit is a light source for irradiating specific light to the outer circumferential surface of the rotating drum. In this Embodiment 1, the light source 410 is fixed to the inner side surface of the cylindrical water tank 402, and the several light source 410 is arrange | positioned so that the light which may contact the outer peripheral surface of a rotating drum may not become discontinuous in the rotation axis direction. have. Since the rotating drum 403 rotates, light can be irradiated to the whole peripheral surface of a rotating drum by this.

In addition, in the case of a structure in which the rotating shaft of the rotating drum is inclined with respect to the supporting axis as shown in Fig. 20, it is preferable to arrange the light source 410 on the upper side (high side of the tank wall surface). When the light source 410 is provided above the water tank, the light source rarely sinks in the water, so the influence by water can be reduced.

As specific light irradiated to the light source 410, it is set as the light which has a peak wavelength in the range of 300 nm-600 nm, and whose irradiation intensity is 500-500,000 mW / cm <2>. If it is light of this characteristic, the outstanding antimicrobial activity enhancement effect will be obtained.

As the member constituting the light source 410, a light emitting diode (LED), a laser diode (LD), a halogen lamp, or the like can be used. Although one light source 410 may be sufficient and it may be arranged in multiple numbers, in order to improve the performance of light irradiation with a small number, it is good to use a reflecting plate (refer FIG. 26). As a method of fixing the light source 410 to the inner wall surface of a water tank, what is necessary is just to fix it with a bolt nut, for example.

In this embodiment, an LED having a peak wavelength of 525 nm is used as the light source 410. This is because light around 525 nm is difficult to be absorbed by water, and is safe for the human body and excellent in economical efficiency. On the other hand, since light on the shorter wavelength side than 525 nm is absorbed by water, the light irradiation efficiency is deteriorated and there is a high possibility that the light itself may damage the device or the human body.

Next, a series of operations using the rotating drum type washing machine of this structure will be described.

The general washing operation using this washing machine is as follows

(1) ⇒ (2) ⇒ (3) ⇒ (4). Or (1)-(2)-(3)-(4 ')-(4 ") and (5)-(6) performed as needed.

(1) This washing of laundry (washing process).

(2) Rinsing washing (rinsing step) performed by pouring silver ionized water into laundry.

(3) Dehydration of laundry (dehydration step).

(4) The laundry is taken out from the rotating drum (takeout step).

Or (4 ') blow drying with heated air or dehumidified air (drying process)

(4 ") The laundry is taken out from the rotating drum (ejection step).

(5) Washing in the washing tank which injects silver ionized water into a rotating drum (washing tank cleaning process).

(6) Drying in a washing tank which blows a heating air or dehumidification air into a rotating drum (internal drying process).

The flow of the above (4 ') to (4 ") is a flow in the case where drying of clothes is also performed in a washing machine, and this flow is not necessarily required.

In addition, the flow of (5)-> (6) is a flow in the case of washing only in the washing tank for sufficient time. This flow does not have to be performed at every washing. Only when it is especially necessary (for example, once a month), it is preferable to perform a washing tank cleaning process using a higher concentration (for example, 600 ppb to 1100 ppb) of silver ionized water and irradiating light for 30 minutes or more. Moreover, it is good to make the usage-amount of silver ion water small enough that the outer peripheral surface of a rotating drum will be damp.

In the rinsing step or the dehydration step or the rinsing step and the dewatering step performed during normal washing, the inside of the washing tank is wet with silver ionized water. At this time, the light irradiation unit is driven to irradiate the inside of the washing tank. Thereby, not only the laundry but also the antibacterial or antibacterial action in the washing tank can be achieved.

As a density | concentration of the silver ionized water used at the said rinsing process, Preferably it is 30 ppb-100 ppb. If the rinsing step is performed with silver ionized water, the clothing is silver ion coated, and thus an effect that the bactericidal effect is maintained even during dehydration drying and wearing is obtained.

(Example 2)

21 shows a rotary washing machine according to the second embodiment. It is a cross-sectional schematic diagram for demonstrating the whole washing machine.

As shown in FIG. 21, in the rotary washing machine according to the second embodiment, a third irradiation unit 430 for irradiating specific light into the rotating drum 403 is disposed in the washing machine door 405, and the water tank 402 is provided. The light source 411 is also disposed on the inner bottom surface in addition to the inner side surface, and is different from the first embodiment. Other than these, it is the same as that of the said Example 1.

As shown in FIG. 21, the washing machine door 405 of Example 2 has a concave shape in which the center part is recessed to the rotating drum side, and the concave bottom part is formed of transparent glass.

Moreover, rubber packing (not shown) is arrange | positioned in the outer frame 401 and the opening part of the water tank 402 which the outer peripheral parts of the washing machine door 405 contact, and when the washing machine door 405 is closed, 402 is to be sealed.

The third irradiation section 430 includes a light source 431 and a reflecting plate 432 that reflects light emitted from the light source to the rotating drum side. As the light source 431, an LED capable of outputting light having a peak wavelength of 525 nm is used, and a reflector 424 is made of a mirror surface of aluminum or stainless steel. These members are fixed to the concave bottom part of the washing machine door 405 with an adhesive, for example.

As in the case of the rotary drum type washing machine with the antibacterial treatment function, the silver ionized water is supplied into the rotating drum 403 in the step of rinsing laundry (rinsing step), in the same manner as in the first embodiment, to the rinsing step and this During the subsequent dehydration process, specific light is irradiated from the first irradiation section and the second irradiation section.

According to the rotating drum type washing machine of this structure, since specific light is directly irradiated to the inside of the rotating drum 403, the disinfection effect with respect to laundry is high, and at the same time, both the inside and the outer peripheral surface of the rotating drum 403 can be sterilized. have.

(Example 3)

The third embodiment is characterized in that the specific light can be irradiated to the outer circumferential surface and the entire surface of the bottom surface of the rotating thrust 402 and the specific light also touches the entire inner surface of the cylindrical water tank 402. Has

Example 3 is demonstrated based on FIGS. 22-24. 22 is a schematic sectional view of the rotary drum type washing machine according to the present embodiment. Fig. 23 is a perspective view of the outer bottom surface of the rotating drum, and Fig. 24 is a cross-sectional view taken along the line X-X of the washing tank (rotating drum + bath).

As shown in FIG. 22, also in this embodiment, the 3rd irradiation part 430 for irradiating specific light in the rotating drum 403 is arrange | positioned at the washing machine door 405. FIG.

On the other hand, in this embodiment, as shown in FIG. 24, the some light source 409 is arrange | positioned along the axial direction in the position which divided the inner periphery of the cylindrical water tank 402 by three.

22 and 23, a strip-shaped reflection region 440 is provided on the outer circumferential surface of the rotating drum 403, and a strip-shaped reflection region 441 is provided on the outer bottom surface of the rotating drum. have.

In the rotating drum type washing machine of this structure, the light emitted from the third irradiation unit 430 is irradiated into the rotating drum 403, and the light emitted from the light source 410 占 411 illuminates the entire outer surface of the rotating drum.

In addition, with the rotation of the rotating drum, the light emitted from the light sources 410 · 411 is reflected by the reflection areas 440 · 441 provided on the peripheral surface and the outer bottom surface of the rotating drum 403, and the water tank 402 Shine inside of). Therefore, the rotating drum type washing machine of this structure can irradiate specific light to the front and back surface of a rotating drum, and the inner whole surface of a water tank.

Also in this washing machine, when silver ionized water is supplied into the rotating drum 403 in the same manner as in the first to second embodiments, the silver ionized water comes out of the hole 405 by the rotation centrifugal force of the rotating drum. As a result, the inner wall of the tank and the outer surface of the rotating drum are wetted with silver ionized water, and the irradiation effect of the specific light is exerted, thereby exhibiting excellent antibacterial activity with lower concentration of silver ions.

The operation of the washing machine according to the present embodiment is the same as that of the first embodiment.

Here, the reflection area formed in the outer surface of a rotating drum is demonstrated.

The reflective region formed on the outer surface of the rotating drum may be formed by, for example, attaching a reflective member such as aluminum foil or stainless steel foil to a predetermined portion by welding or a water resistant adhesive.

There is no particular limitation on the form of the reflective region, but a form capable of reflecting light from the light irradiating portion to spread evenly on the inner surface of the tank is preferable. For example, as shown to Fig.25 (a), it forms in strip shape in the direction orthogonal to a rotation direction on the outer peripheral surface of a rotating drum. It is preferable to provide a band-shaped reflection area from the bottom surface to the opening because, when the rotating drum rotates once, the reflected light is irradiated on the entire surface of the outer circumferential surface of the rotating drum.

In addition, as shown in Fig. 25B, the reflection area is formed in a spiral shape with respect to the rotational direction of the rotating drum. According to this aspect, the reflection time accompanying rotation can be lengthened.

In addition, as shown in Fig. 25C, the reflection region may be dotted with island shapes on the outer peripheral surface of the rotating drum. It goes without saying that the entire outer surface of the rotating drum may be reflected.

Furthermore, reflective regions are interspersed in an island shape on the entire surface outside the rotating drum, and the shapes of the respective reflective regions are convex. An island shape is preferable because less reflection material is finished, and if the shape is convex, the light is diffusely reflected in various directions so that the light is evenly distributed to every corner of the tank inner surface. At this time, it is preferable that the convex island-shaped reflection area is arrange | positioned spirally toward the rotation direction of a rotating drum. With this structure, light is diffusely reflected by the rotation of the rotating drum, and the reflected light is uniformly irradiated onto the inner surface of the tank. Thereby, the antibacterial treatment without a nonuniformity can be performed.

Although the example of the reflection area | region provided in the rotating drum outer side bottom surface was shown to FIG. 23 (441), the above thought can also be applied also to the reflection area provided in the rotation drum outer bottom surface. That is, a reflection area can be provided in a spiral shape from a rotation center, or a reflection surface can be made into an uneven shape.

In addition, in the present embodiment, the light source is disposed on the inner surface of the water tank, and the reflection area for reflecting light from the light source is provided on the outer surface of the rotating drum, but the light source is provided on both the inner surface of the water tank and the outer surface of the rotating drum. Alternatively, a light source for irradiating light toward the tank side may be provided on the outer surface of the rotating drum and a reflecting area may be provided on the inner surface of the tank.

In addition, although the 1st irradiation part and the 2nd irradiation part 450 were only the light source 410 and the light source 411 in FIGS. 20-22, the light irradiation part is shown with the light source 410 as shown in FIG. A reflector plate 451 for widening the light irradiation range and a waterproof and light transmissive protective cover 452 may be provided. When the light irradiation part is fixed to the wall with a fixing member such as a screw or an adhesive, do. Reference numeral 453 in FIG. 26 denotes an inner bottom surface of the water tank.

(Example 4)

The fourth embodiment is characterized in that a light irradiation part for irradiating light into the rotating drum is built in a baffle provided in the rotating drum, and this point and the third irradiating part 430 in the washing machine door 405. About matters other than that which did not install, it is the same as that of the said Example 3.

27 is an enlarged aiming view of the baffle portion of the rotary drum type washing machine according to the fourth embodiment, and a cross-sectional schematic diagram illustrating a light irradiation part built into the baffle is shown in FIG. 28.

The main body front and side surfaces of the baffle 460 are formed of a light-transmissive resin material, and a light source 461 composed of a reflecting plate 462 made of an aluminum mirror surface plate and an LED emitting light having a peak wavelength of 525 nm. (And power supply: not shown) is built-in light irradiation.

The baffle 460 has a structure in which water does not penetrate the inside thereof, and is held in a metal frame made of a resin material for increasing its strength and for convenience of mounting on a rotating drum, and is provided with screws, bolts, and the like. It is attached to the rotating drum wall surface by the fixing member of the.

In this embodiment, a cylindrical rotating drum having an inner diameter of 50 cm x 60 cm is used, and three baffles of 57 cm in length, 5 cm in width and 5 cm in height are parallel to the drum in this direction. Moreover, it is arrange | positioned at equal intervals in the circumferential direction of a rotating drum.

As shown in Fig. 27, three LEDs are built into each baffle.

In addition, the power supply to the light source 461 accommodated in the baffle is structured through the power lead (not shown) arrange | positioned along the drum outer wall from the motor 404 side which rotationally drives a drum. It is also possible to use a battery power source.

In the present embodiment, light having a peak wavelength of 525 nm is used as the light generated by the light irradiation 461. This wavelength light is especially preferable at the point which is hard to be absorbed by water. However, the light generated by the light irradiation 461 may be light having a peak wavelength of 300 nm to 600 nm.

Here, even when light having a wavelength of 525 nm is present, a large amount of water attenuates the light intensity. On the other hand, a large amount of silver ionized water is not necessary for the antimicrobial activity of microorganisms, and silver ions of about 10 ppb to about 1100 ppb may be present in the vicinity of the microorganisms. In consideration of the cost / performance point and the low load on the natural environment, it is preferable to perform antibacterial treatment with a small amount of silver ionized water. In view of these points, in this embodiment, in the final step of the rinsing step, 10 L of silver ionized water having a concentration of 60 ppb is introduced into the rotating drum to perform a 10-minute rinse finishing step and a 20-minute dehydration step. All the light irradiation parts 410, 411, 461 were made to operate.

In this setting, a drive control unit having a computer is provided, the setting program is stored in the computer, and executed by the drive control unit.

Moreover, in the rotary drum type washing machine which concerns on a present Example, it was set as the structure which an operator can perform a washing tank cleaning process by giving an instruction | indication to the said drive control part.

The washing tank cleaning step refers to any step performed to more strongly antibacterial the inside of the washing tank. Specifically, for example, 10 L of silver ionized water having a concentration of about 900 to 1100 ppb is poured into the rotating drum, and the rotating drum is rotated for about 20 to 60 minutes while the entire light irradiation unit is turned on. Thereby, the whole antibacterial treatment in the washing tank including the back side of a rotating drum is performed. This washing tank cleaning process is performed about once a month, for example, and when a contamination is severe, it is performed about once a week.

When this washing tank cleaning process is performed using silver ionized water of 900-1100 ppb concentration, a small amount of silver ionized water (so that the inside of a washing tank can be wetted) is enough, and after an antibacterial treatment with silver ionized water, if necessary, It is preferable to wash the inside of the washing tank with a relatively large amount of water (3-5 minutes of washing time), and then dry the inside of the washing tank.

By carrying out these antibacterial treatments, actinomycetes, Staphylococcus aureus, Escherichia coli, photosynthetic bacteria (prokaryotes), eukaryotic organisms such as Cladosporium, ringworm, yeast bacteria such as Candida, pathogenic viruses, etc. It can be sterile or antibacterial.

In addition, instead of silver ionized water, or together with silver ions, ionized water containing copper ions and / or zinc ions can be used.

28 is a conceptual diagram which shows one form of the baffle in which the light irradiation part was built, and the baffle in which the light irradiation part was built is not limited to the form shown in this figure.

There is no particular limitation on the shape of the baffle, the installation position, and the inclination angle with respect to the rotational direction. Usually, although the some baffle which is elongate in the direction parallel to a rotating shaft is provided in a rotating drum, in order to make light wash light completely, it is good to incorporate the light irradiation part in all those baffles. In addition, it is preferable to allow light to be irradiated from both the entire surface and the side surface of the baffle 460. On the other hand, it is better to incorporate a light irradiation part in a part from the viewpoint of cost.

As a resin material which comprises a baffle main body, Preferably, resin with high transmittance | permeability with respect to the light whose wavelength is 525 nm is used, As light irradiation intensity | strength, the light intensity irradiated to laundry shall be 500-500,000 mW / cm <2>.

(Confirmation test 1 of antibacterial treatment effect)

Using the rotary drum type washing machine which concerns on the said Example 4, the confirmation test of the antimicrobial effect in antibacterial treatment operation was done. The test method is as follows.

(1) Candida albicans NBRC-1060 standard strain (eukaryote) was prepared as a microorganism to be subjected to antibacterial treatment. As an initial cell number, cells of about 1 × 10 ⁶ to 5 × 10 ⁷ / mL were dispersed in a phosphate buffer (concentration 50mM) to prepare a spore suspension. Then, 1 mL of the spore suspension was inoculated into an exempt test cloth (36 cm 2; Japanese Standards Association Kanekin No. 3). This test cloth is referred to as a fouling test cloth.

(2) Using the water-resistant adhesive, the contaminated test cloth was used for the inner peripheral surface (test part B1) and inner bottom surface (test part B2) of the water tank, the outer peripheral surface (test part C1) and the outer bottom surface (test part C2) of the rotating drum. The antimicrobial treatment effect in each part of the washing tank was investigated by the method of adhering to and attaching a contaminated test cloth into a rotating drum (test site A).

The said test part B1 was made into the center point vicinity made from both the depth direction and the width direction of the water tank in sectional drawing of the washing machine shown in FIG.

The test site C1 was set near the center point made from both the depth direction and the width direction of the rotating drum in the sectional views shown in FIG. 20 and the like.

Test site B2 was positioned above the center of the bottom of the circular water tank. Test site C2 was set near the outer edge of the circular bottom surface of the rotating drum.

In addition, each site | part was made the same and the test was repeated in antibacterial treatment conditions (below).

[2-1] (water only)

While rotating the rotating drum at 50 rpm, 10 L of tap water was put in the rotating drum, and all the light irradiation parts were turned off and rotated for a predetermined time. Each test cloth was withdrawn after a predetermined time of rotation. Predetermined time was 15 minutes, 30 minutes, and 60 minutes. Let these be sample G1 (sample group 1).

When 10 L of water was poured into the rotating drum and the drum was rotated at 50 rpm, water was scattered to the outside of the rotating drum by centrifugal force, and it was confirmed that the contaminated test cloth attached to each part of the washing tank was wetted with water.

[2-2] (water + specific light)

All the light irradiation parts were turned on, and the specific light of peak wavelength 525nm and irradiation intensity 50 dB / cm <2> was irradiated to the whole washing tank. Other than this, it carried out similarly to said (2-1). Let these be sample G2.

[2-3] (only silver ionized water)

Instead of tap water, the same procedure as in [2-1] was conducted except that silver ionized water having a concentration of 60 ppb was used. Let these be sample G3.

[2-4] (silver ionized water + specific light)

It carried out similarly to the said [2-2] except having used silver ionized water of 60 ppb concentration instead of tap water. Let these be sample G4.

(3) After the operation in each of the above conditions, the contamination test cloth was collected. Cells were extracted from the recovery test cloth, the extract was plated on potato agar medium, incubated at 25 ° C for 4 days, and the number of colonies on the medium was counted. Thereby, the antimicrobial effect in each condition was investigated.

(Confirmation test 2 of antibacterial treatment effect)

Instead of Candida albicans NBRC-1060, cladosporium cladosporioides (NBRC 6348) was used, and the silver ion concentration was set to 100 ppb. Other than this, confirmation test 2 was performed in the same manner as the confirmation test 1 above.

(Confirmation test 3 of antibacterial treatment effect)

In place of the Candida albicans NBRC-1060, except for using Escherichia coli (NBRC-3972, Escherichia coli), confirming test 3 was carried out in the same manner as the confirming test 1.

(Test result)

(1) The result of confirmation test 1 in test site A is shown in FIG. From Fig. 29, the sample G4 (○ in the figure) irradiated with specific light in the state of contacting silver ionized water with the contaminated test cloth contaminated with Candida albicans was sample G3 in which silver ionized water was contacted without irradiating specific light. It was recognized to exhibit a sterilization effect of one or more orders of magnitude higher than (in the drawing).

Moreover, it was recognized from the comparison of sample G2 (Δ in the figure) and sample G1 (▲ in the figure) that there was no antibacterial effect at all by irradiation of specific light alone.

30, the antibacterial effect of confirmation test 1 and sample G4 in each part of a washing tank is shown by the bar graph.

It was recognized from FIG. 30 that it showed the substantially equivalent antibacterial effect with respect to the contamination completion test cloth arrange | positioned at each site | part of a washing tank.

(2) In FIG. 31, the antimicrobial result of sample G4 using the test cloth contaminated with Cladosporium cladosporioides is shown, and the antimicrobial result of the sample G4 using the test cloth contaminated with Escherichia coli is shown in FIG.

From the results of FIGS. 31 and 32, it was confirmed that the same antimicrobial effect was obtained for any microorganism.

In addition, the above result can be considered as follows. The irradiation intensity of specific light used in the rotary drum type washing machine with an antibacterial treatment function according to the present invention is sufficiently lower than the strength capable of directly antibacterial fungi. Therefore, the result of G4 does not arise because the antimicrobial activity of light was added to the antimicrobial activity of silver ion, but because the specific light accelerated | introduced the introduction of metal ion into a cell.

[First invention group]

According to the antimicrobial method of the present invention, since the antibacterial power of metal ions is enhanced, the growth of microorganisms can be prevented at a lower concentration of metal ions. Therefore, by using such an antibacterial method of the present invention, it is possible to realize human-friendly antibacterial with little contamination to the natural environment. Therefore, the industrial significance is large.

[2nd invention group]

The method and apparatus for controlling cell activity according to the second invention group can be applied to cancer patients, for example, and by application of the present invention, ionic species expressing antitumor action can be efficiently introduced only into malignant tumor cells. have. Thereby, it is possible to selectively kill only malignant tumor cells. Therefore, the industrial applicability is great.

[Third invention group]

Since the inside of the piping of the ice making apparatus provided in the refrigerator is not easy to clean, microorganisms, such as mold and staphylococci, may multiply by the use of a long time, and the discovery is not easy. According to the invention of the third invention group, fungi, staphylococci, and the like, which propagate near water in pipes and the like, can be automatically and antibacterially cleaned using a very small amount of silver ions, and the occurrence thereof can be prevented in advance. Therefore, the industrial applicability is great.

[4th invention group]

If the rotary drum type washing machine provided with the antibacterial treatment function which concerns on the 4th invention group which can supply silver ionized water to a washing tank automatically, and has a light irradiation part which can irradiate specific light automatically to the whole surface of a washing tank, a specific light is Since the introduction of metal ions into the cells is promoted, it is possible to automatically disinfect or antibacterial the laundry and the entire washing tank with a lower concentration of silver ionized water. Such industrial applicability of the present invention is great.

Claims (84)

It is an antimicrobial method that inhibits the growth of microorganisms using antimicrobial metal ions, Contacting the microorganism with antimicrobial metal ions after or while irradiating a particular light to the microorganism, The specific light is light having a peak wavelength in a range of 300 nm or more and 600 nm or less. delete The method according to claim 1, wherein the antimicrobial metal ions are silver ions contained in a silver ion-containing solution having a concentration of 1100 ppb or less, The irradiation intensity of the said specific light is 1,000 mW / cm <2> or more, The antibacterial method characterized by the above-mentioned. It is an antimicrobial method that inhibits the growth of microorganisms using antimicrobial metal ions, Irradiating a specific light to the microorganism in the state of contacting the microorganism with the antimicrobial metal ion, The specific light is light having a peak wavelength in a range of 300 nm or more and 600 nm or less. delete The method according to claim 4, wherein the antimicrobial metal ions are silver ions contained in a silver ion-containing solution having a concentration of 1100 ppb or less, The irradiation intensity of the said specific light is 1,000 mW / cm <2> or more, The antibacterial method characterized by the above-mentioned. Ion generating means for generating antibacterial metal ions, Contact means for contacting the microorganisms with the antimicrobial metal ions generated by the antimicrobial metal ion generating means; Irradiation means for irradiating specific light to the said microorganism in the state which the antimicrobial metal ion contacted the microorganism by the said contact means, The specific light is light having a peak wavelength in a range of 300 nm or more and 600 nm or less. The antimicrobial device according to claim 7, wherein the specific light irradiation intensity is 500 to 500,000 mW / cm 2. delete The antimicrobial device according to claim 8, wherein the antimicrobial metal ions are silver ions. Ion generating means for generating antibacterial metal ions, Irradiation means for irradiating specific light to the microorganism, Contacting means for contacting the microorganism to which the specific light is irradiated with the antimicrobial metal ions generated by the ion generating means, The specific light is light having a peak wavelength in a range of 300 nm or more and 600 nm or less. The antimicrobial device according to claim 11, wherein the specific light irradiation intensity is 500 to 500,000 mW / cm 2. delete The antimicrobial device according to claim 12, wherein the antimicrobial metal ions are silver ions. A light source unit for outputting light, An irradiation unit for inducing the light output from the light source unit to the animal cell and irradiating the light to the animal cell, and A contact portion for contacting the animal cell with an antimicrobial metal ion, The specific light has a peak wavelength in the range of 300 nm or more and 600 nm or less, and the irradiation intensity is 500-500,000 mW / cm 2. It is a refrigerator provided with the automatic ice-making apparatus which has the automatic antibacterial treatment function which can prevent the growth of microorganisms in a water pipe, The automatic antibacterial treatment comprises both irradiating a specific light to the microorganism, and contacting the antimicrobial metal ion to the microorganism, The automatic ice maker includes an ice maker, a water supply tank, A water pipe guiding the water accumulated in the water supply tank to the ice making unit; An ion supply unit for supplying antimicrobial metal ions to the water supply tank or to the water pipe; The light source unit which irradiates the said specific light in the said water supply tank or the said water pipe is provided, The specific light has a peak wavelength in a range of 300 nm or more and 600 nm or less, and the irradiation intensity is light of 500 to 500,000 mW / cm 2, Refrigerator with automatic ice maker with automatic antibacterial treatment. It is a refrigerator provided with the automatic ice-making apparatus which has the automatic antibacterial treatment function which can prevent the growth of microorganisms in a water pipe, The automatic antibacterial treatment comprises both irradiating a specific light to the microorganism, and contacting the antimicrobial metal ion to the microorganism, The automatic ice making device includes an ice making unit, a water pipe leading to water to the ice making unit, An ion supply unit for supplying antimicrobial metal ions to the water pipe; The light source part which irradiates a specific light to the said water pipe is provided, The specific light has a peak wavelength in a range of 300 nm or more and 600 nm or less, and the irradiation intensity is light of 500 to 500,000 mW / cm 2, Refrigerator with automatic ice maker with automatic antibacterial treatment. It is a refrigerator provided with the automatic ice-making apparatus which has the automatic antibacterial treatment function which can prevent the growth of microorganisms in a water pipe, The automatic antibacterial treatment comprises both irradiating a specific light to the microorganism, and contacting the antimicrobial metal ion to the microorganism, The automatic ice making device includes an ice making unit, a water pipe leading to water to the ice making unit, A light source unit for irradiating specific light to the water pipe; The water pipe comprises a silver ion eluting material which elutes silver ions, The specific light has a peak wavelength in a range of 300 nm or more and 600 nm or less, and the irradiation intensity is light of 500 to 500,000 mW / cm 2, Refrigerator with automatic ice maker with automatic antibacterial treatment. It is a refrigerator provided with the automatic ice-making apparatus which has the automatic antibacterial treatment function which can prevent the growth of microorganisms in a water pipe, The automatic antibacterial treatment comprises both irradiating a specific light to the microorganism, and contacting the antimicrobial metal ion to the microorganism, The automatic ice making apparatus includes a water supply tank, an ice making dish, and a water pipe for guiding water from the water supply tank to the ice making dish, At least one member of the water supply tank, the ice tray, and the water pipe includes a silver ion eluent that elutes silver ions, In the vicinity of the member, a light source unit for irradiating the specific light to the water pipe is provided. The specific light has a peak wavelength in a range of 300 nm or more and 600 nm or less, and the irradiation intensity is light of 500 to 500,000 mW / cm 2,  Refrigerator with automatic ice maker with automatic antibacterial treatment. A refrigerator further comprising a refrigerating compartment and a freezing compartment separate from the refrigerating compartment and having an automatic anti-icing device capable of preventing the growth of microorganisms in the water pipe; The automatic antibacterial treatment comprises both irradiating a specific light to the microorganism, and contacting the antimicrobial metal ion to the microorganism, The automatic ice maker includes: a water supply tank disposed in the refrigerating chamber; An ice making plate installed in one compartment of the freezer compartment, A water pipe guiding the water accumulated in the water supply tank to the ice tray; An ion supply unit for supplying antimicrobial metal ions in the water supply tank or in the water supply tank, The water pipe is light-transmissive, and a light source portion for irradiating the water pipe is provided near the water pipe. The specific light has a peak wavelength in a range of 300 nm or more and 600 nm or less, and the irradiation intensity is light of 500 to 500,000 mW / cm 2,  Refrigerator with automatic ice maker with automatic antibacterial treatment. 21. The automatic ice making device according to claim 20, wherein the water supply tank of the automatic ice making device is light transmissive, and a light source unit for irradiating a water pipe to a specific light is provided near the water supply tank. One refrigerator. It is a rotary drum type washing machine which prevents microbial contamination at the time of washing, and can perform antibacterial treatment to laundry, The microbial contamination prevention and antimicrobial treatment comprises both the step of irradiating a specific light to the microorganism, and the step of contacting the antimicrobial metal ion to the microorganism, A tank capable of receiving water and discharging the received water; A rotary drum disposed in the water tank and rotationally driven in the water tank; Is a rotary drum type washing machine having at least an ion supply means for supplying ionized water containing antimicrobial metal ions in the rotary drum or the water tank, The rotary drum has a plurality of holes in the wall surface through which water can travel between the rotary drum and the tank. The tank includes a first irradiation part that irradiates specific light to an outer surface of the rotating drum on an inner surface thereof, The said specific light is light which has a peak wavelength in the range of 300 nm or more and 600 nm or less, The rotating drum type washing machine characterized by the above-mentioned. 23. The rotating drum type washing machine according to claim 22, wherein the rotating drum includes a reflection area that reflects the specific light on an outer surface thereof. The rotating drum type washing machine according to claim 23, wherein the reflective region is formed in a band shape in a direction orthogonal to the rotation direction of the rotating drum. The rotating drum type washing machine according to claim 23, wherein the reflective region is formed in a spiral shape with respect to the rotating direction of the rotating drum. The rotating drum type washing machine according to claim 23, wherein the reflective region is formed by being dotted with island shapes on the outer surface of the rotating drum, and the shape of each reflective region is convex. The said light irradiation part consists of a 1st irradiation part provided in the inner side surface of the said tank, and a 2nd irradiation part provided in the inner bottom surface of the said tank, The said reflection area | region is provided in both the side surface and the bottom surface of a rotating drum, The rotating drum type washing machine characterized by the above-mentioned. The method of claim 22, The ion supply means is a silver ion supply means for supplying silver ions, Rotating drum type washing machine, characterized in that the irradiation intensity of the specific light is 500 to 500,000 mW / cm 2. It is a rotary drum type washing machine which prevents microbial contamination at the time of washing, and can perform antibacterial treatment to laundry, The microbial contamination prevention and antimicrobial treatment comprises both the step of irradiating a specific light to the microorganism, and the step of contacting the antimicrobial metal ion to the microorganism, A tank capable of receiving water and discharging the received water; A rotary drum disposed in the water tank and rotationally driven in the water tank; At least silver ion supply means for supplying silver ion water in the rotating drum or in the water tank, The rotary drum has a plurality of holes through which water can flow between the rotary drum and the tank, On the inner wall of the rotating drum, a baffle for rolling laundry is formed, The light baffle which irradiates light in the rotating drum is built in the said baffle, The said specific light has a peak wavelength in the range of 300 nm or more and 600 nm or less, and the irradiation intensity is the light of 500-500,000 mW / cm <2>, The rotating drum type washing machine characterized by the above-mentioned. The said water tank is provided with the 1st irradiation part which irradiates light to the outer peripheral surface of the said rotating drum in the inner side surface, and the inner bottom surface, the light is applied to the outer bottom surface of the said rotating drum. It is provided with the 2nd irradiation part to irradiate, The rotating drum type washing machine characterized by the reflection area provided in both the outer peripheral surface and the outer bottom surface of the said rotating drum. delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete

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