KR101095965B1 - Tooth paste comprising of mistletoe extract - Google Patents

Abstract

The present invention relates to a toothpaste composition, which provides an antimicrobial effect against gums and periodontal disease and Helicobacter pylori bacteria, as well as anti-inflammatory toothpaste composition, characterized in that it contains an extract of Korean mistletoe which has an immuno-boosting effect as an active ingredient. Has an excellent effect.

Toothpaste, Mistletoe, Periodontal Disease, Gum Disease, Helicobacter Pylori, Immune Enhancement

Description

Tooth paste comprising of mistletoe extract

The present invention relates to a novel toothpaste composition comprising a mistletoe extract having an immune enhancing effect. More specifically, by adding Korean mistletoe having an immune boosting and antimicrobial effect as an active ingredient to a conventional toothpaste composition, it stimulates mucosal immunity in the mouth and suppresses the formation of plaque that causes periodontal disease, thereby maintaining the cleanliness of the mouth. It relates to a toothpaste composition having antimicrobial efficacy against Helicobacter pylori (Helicobacter pylori) at the same time.

Plaque is a caustic acid-toxic substance that is an invisible transparent membrane that is the most direct cause of oral disease. It mainly produces acid on the surface of the tooth, which destroys the enamel, which is the hard part of the tooth, leading to tartar and tooth extraction and gum disease. In particular, oral bacteria such as Streptococcus mutans increase the fermentation of food and drop the pH in the mouth from neutral to weak acid, which is the main cause of bad breath, plaque formation and periodontal disease.

 As a toothpaste composition for the prevention of bad breath, periodontal disease and gum disease, a method containing 0.5 to 4% by weight of activated carbon fiber is disclosed in Korean Patent Registration No. 10-0765473, especially as a toothpaste composition for treating periodontitis It is disclosed in Korean Patent No. 10-0829519 characterized in that it contains as an active ingredient.

In an effort to prevent plaque formation, research on toothpaste compositions using herbal medicines and compounds has been actively conducted. However, since the toothpaste has only 50% of the plaque removal efficiency of the conventional toothpaste, there is an urgent need for a toothpaste having excellent efficacy in suppressing plaque formation and oral bacteria.

In addition, gum disease, such as oral disease, gingivitis, and bad breath inhibitory toothpaste composition was disclosed in Korea Patent No. 10-0811594 by adding 3 to 9% by weight of rock salt.

On the other hand, Helicobacter pylori, a typical viscera causative agent, is a Gram-negative bacterium, microaerophilic, difficult to culture, and chronic inflammation of the gastric mucosa in the process of penetrating the gastric mucosa in the process of forming a colony of the stomach. In addition to causing chronic active gastritis in humans as well as parasitic gastric mucosa, gastrointestinal ulcers, gastric adenocarcinoma, and gastrointestinal lymphoma are associated with various gastrointestinal disorders. The infection rate of Helicobacter pylori is about 60 ~ 75%, which is very high compared to other countries in the West. Therefore, various methods for disinfecting Helicobacter pylori have been tried, but many side effects and low antibacterial effects are often prevented and treated. It is difficult.

Mistletoe (Misttletoe, Viscum album) is a semi-parasitic plant that grows with various trees as a host. In oriental medicine, pain relief such as back pain and toothache, improvement of blood circulation such as hypertension and arteriosclerosis, prevention of miscarriage and removal of tumor Has been used. Recently, studies on the anti-cancer and immune activity of mistletoe extracts are underway, especially the lectins contained in mistletoe are known to have various immunological functions. A paper has been published. In addition, Korean mistletoe lectin is widely used as adjuvant, an adjuvant for the production of immunized antibodies in oral administration or intraperitoneal inoculation.

The present invention relates to a toothpaste composition having an excellent antimicrobial effect against plaque formation and Helicobacter pylori by supplementing the weakness of the conventional toothpaste, and relates to an antibacterial and immunologically functional toothpaste composition.

An object of the present invention is to provide a functional toothpaste composition having suppression of periodontal disease and gum inflammation by adding a substance having antimicrobial efficacy against plaque formation and visceral disease bacteria as an active ingredient as described above.

The object of the present invention was achieved by preparing the mistletoe extract and obtaining the functional toothpaste composition and then verifying the antimicrobial effect, survival inhibition effect on Helicobacter pylori, and plaque formation inhibition effect.

As is apparent from the Examples and Experimental Examples described below, the toothpaste composition comprising the Korean mistletoe extract having immuno-boosting efficacy is the main causative bacterium of periodontal disease and virulence of visceral disease compared to the comparative example. Excellent antibacterial effect and excellent plaque formation inhibitory effect is very useful invention in the novel functional toothpaste industry.

Hereinafter, with reference to the drawings attached to the most preferred specific configuration and operation in order to practice the present invention.

The present invention confirms the change in the basic efficacy of the toothpaste by adding Korean mistletoe to the conventional toothpaste composition, and then compare the antioxidant efficacy of the toothpaste including Korean mistletoe compared to the conventional toothpaste to help maintain the pH in the oral cavity to neutral Confirming whether or not to give; Measuring the MIC of antimicrobial efficacy against typical bacteria causing periodontal disease and comparing the degree of plaque formation and comparing the antimicrobial efficacy against Helicobacter pylori among the causative agents of visceral diseases; Comprising the step of providing a functional toothpaste composition comprising a Korean mistletoe extract as an active ingredient based on the results of the antimicrobial efficacy against the causative agents of antioxidant and periodontal disease and visceral diseases according to the present invention.

However, the present invention has been described in detail through Examples, Comparative Examples, etc. using the Korean mistletoe extract to confirm the function of the toothpaste composition having immuno-enhancing efficacy, the scope of the present invention is limited to these Examples, Comparative Examples only It doesn't happen.

For example, it goes without saying that it belongs to the scope of the present invention to manufacture a functional toothpaste composition within the scope of the present invention, which contains the mistletoe extract of European, American, etc. as an active ingredient.

Example  1: Preparation of Mistletoe Extract

As the extraction method of mistletoe performed by the inventors of the prior art, three types of hot water extraction methods, cold water extraction methods, and a mixed method thereof were prepared as shown in FIG. 1.

In the present invention, 1 liter of water was added to 200 g of Korean mistletoe, heated at 100 ° C. for 30 minutes to obtain a hot water extract, and then lyophilized to obtain a hot water extract powder, which was named HKM-110.

1 L of water was added to 200 g of Korean mistletoe and stirred at 4 ° C. for 2 hours to obtain a cold water extract, which was then lyophilized to obtain a cold water extract powder, which was named KM-110 (or Mislo-C).

After obtaining the cold water extract, 1 liter of water was added to 200 g of the remaining Korean mistletoe residue, heated at 100 ° C. for 30 minutes to obtain a hot water extract, and then lyophilized to obtain a hot water extract powder, which was named Mislo-H. In the toothpaste composition of the present invention, Mislo-C was used as a test material.

Example  2: Toothpaste Composition of the Invention

Three examples (Samples) were prepared by varying the concentration of the Korean mistletoe extract (Mislo-C) extracted in Example 1 in conventional toothpaste compositions such as wetting agents, abrasives, binders, sweeteners, preservatives, and fragrances. 1).

TABLE 1

Example  3: antibacterial effect

The following two experiments were carried out to determine the antimicrobial effect of the toothpaste composition of the present invention against oral bacteria.

[ Experimental Example  One]

MIC was measured for Streptococcus mutans, a causative causative agent, and porphyromonas gingivalis, a causal causative agent. The measurement method was measured using a liquid dilution method. First, the toothpaste composition was diluted 100-fold in purified water, diluted 2-fold step up to 28 in 5 ml of liquid medium, and then inoculated with bacteria. After incubation for 3 hours in an environment of 37 DEG C and 5% CO _2, the temperature at which no growth of the strain was observed was determined as MIC. MIC determination results are shown in Table 2 below.

TABLE 2

[ Experimental Example  2]

After diluting the dentifrice composition of Comparative Example 2 and the comparative example of the experimental results in a liquid medium and inoculating the bacteria, the growth of the bacteria was observed by measuring the OD value at 660nm at an hourly interval. Results for Porphyromonas gingivalis are shown in FIG. 2, and results for Streptococcus mutans are shown in FIG. 3.

Example  4: Helicobacter Pylori  Survival inhibition effect

Survival inhibitory effect on Helicobacter pylori was carried out in the same manner as in Experimental Example and the MIC for Helicobacter pylori was measured. The measurement results are shown in Table 3 below.

TABLE 3

Example  5: Prague  Formation inhibitory effect

In order to measure the plaque formation inhibitory effect of the present invention toothpaste composition 30 people with a plaque was selected to brush the toothpaste composition prepared according to the composition examples and comparative examples three times daily for 30 days, at the time of the laboratory Based on the degree of plaque formation of 100%, the rate of plaque formation over time was measured at a 5-day cycle. Experimental results are shown in FIG. 4.

Most functional toothpastes developed so far are related to periodontal disease. The present invention is the first composition having an anti-helicobacter pylori function and an immune function enhancing effect presented in the present invention. Therefore, as described above, the toothpaste composition of the present invention is useful in the biopharmaceutical industry because it has an excellent effect of removing the causative bacteria involved in plaque formation and the causative bacteria of periodontal disease and visceral diseases.

1 is a diagram showing the manufacturing process of the Korean mistletoe extract for toothpaste composition of the present invention.

Figure 2 is a graph showing the antimicrobial effect of Porphyromonas gingivalis of the Korean mistletoe extract containing as an active ingredient of the toothpaste composition of the present invention.

Figure 3 is a graph showing the antimicrobial effect of Streptococcus mutans of the Korean mistletoe extract containing as an active ingredient of the present invention toothpaste composition.

Figure 4 is a graph showing the plaque formation inhibitory effect of the toothpaste composition of the present invention.

Claims (4)

Antimicrobial and plaque formation inhibiting functional toothpaste composition characterized by containing the extract of Mistletoe (Viscum album) as an active ingredient. The antimicrobial and plaque formation inhibiting functional toothpaste composition according to claim 1, wherein the mistletoe extract is 0.5 to 2% by weight. The method according to any one of claims 1 to 2, wherein the antimicrobial function is Streptococcu Toothpaste composition, characterized in that for s mutans, Porphyromonas gingivalis, Helicobacter pyrori strain. delete

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