KR101085540B1 - Cosmetic composition containing IgY from egg yolk for improvement of acene - Google Patents

Abstract

IgY for Propionibacterium acnes , IgY for Propionibacterium propionicus , IgY for Propionibacterium propionicus , Staphylococcus aureus , Staphylococcus aureus Egg yolk containing IgY for Staphylococcus epidermidis , IgY for Micrococcus luteus and IgY for Actinomyces israelii ; At least one primary extract selected from pontoon extract, triticale extract or lettuce extract; At least one secondary extract selected from agrimoni extract or spectral extract; Any one or more tertiary extracts selected from dandelion extract, cotyledon extract, rice extract or bamboo extract; And it relates to a cosmetic composition for improving acne characterized in that it comprises a water-soluble mixture of mixed water; by containing egg yolk with a specific antibody to a variety of causative agents causing acne can effectively prevent and improve acne In addition, the use of natural materials can reduce the consumer's rejection.

Micrococcus Luteus, Stephylococcus aureus, Staphylococcus epidermis, Actinomysis Israeli, Acne, Inflammation, Improvement, Propionibacterium acnes, Propionibacterium propionicus, Antibodies, IgY

Description

Cosmetic composition for improving acne containing egg yolk with antibodies against acne {Cosmetic composition containing IgY from egg yolk for improvement of acene}

The present invention relates to a cosmetic composition for improving acne, and more particularly to a cosmetic composition for improving acne comprising an egg yolk containing a specific antibody against the acne-inducing bacteria.

The medical name of acne is vulgar acne, which increases the production of sebum in the pores, abnormally keratinizes the skin epithelial cells and blocks the openings of the pores, and is caused by the growth of acne-causing bacteria and inflammation.

Acne is a skin disease that occurs mainly in adolescents with secondary sexual maturity, and it is reported that about 80% occur between 11 and 30 years of age, and about 6% of patients are in their 30s and later. Acne may occur after 20s.

The causes of acne can be largely divided into three, the first is the excessive production of sebum. Early acne is triggered by increased hormones in sexual maturation, especially testosterone is the most important hormone, which stimulates sebaceous glands to increase sebum production. Sebum is composed of triglycerols at the time of initial secretion, but gradually the Propionibacterium It is broken down into free fatty acid and glycerol by lipase, one of the extracellular enzymes of acnes ). 95% of the free fatty acids in the skin are caused by this bacterium and induce microvesicle formation and inflammatory reactions. The extracellular enzymes of propionibacterium acnes include lipase, protease, hyaluronidase, etc., which have an important effect on the progression of acne.

Second is the closure of the pores. The nipple is closed due to the hyperkeratosis of the skin affected by the free fatty acids and hormones produced by the action of propionibacterium acnes and the excessive secretion of sebum, thereby facilitating the propagation of the anaerobic bacterium propionibacterium acnes. do.

Third is the increase in the number of anaerobic bacteria, Propionibacterium acnes. If sebum accumulates as a white mass or as a black mass adhered to melanin, Propionibacterium acnes ) is increased.

Although the three factors described above are the biggest causes of acne, in addition to the effects of stress caused by neuroproteins, genetic factors, cosmetics, hyperkeratosis, heat and moisture, ultraviolet rays and other factors are also associated with acne.

On the other hand, antibiotics and steroids are used to treat acne, but they have excellent therapeutic effects during the initial treatment, but have problems such as resistance to long-term use and side effects such as diabetes, hypertension, peptic ulcer or lowered immune function.

Accordingly, the present invention is composed of natural ingredients, there is no objection to people, antibiotic resistance and side effects do not occur, the skin can breathe and do not block pores, so that the waste can easily come out cosmetic composition for improving acne There is this.

In order to achieve the above object, the present invention relates to IgY for Propionibacterium acnes , IgY for Propionibacterium propionicus , and Staphylococcus aureus . Egg yolk containing IgY, IgY for Staphylococcus epidermidis , IgY for Micrococcus luteus and IgY for Actinomyces israelii ; At least one primary extract selected from pontoon extract, triticale extract or lettuce extract; At least one secondary extract selected from agrimoni extract or spectral extract; Any one or more tertiary extracts selected from dandelion extract, self-leaving extract, rice extract or bamboo extract; And purified water; It provides a cosmetic composition for improving acne comprising a water-soluble mixture of the mixture.

Hereinafter will be described in detail with respect to the problem solving means of the present invention.

The present invention is necessarily propionibacterium acnes ( propionibacterium acnes ), propionibacterium propionicus ( propionibacterium propionicus ), Staphylococcus aureus to provide an effective acne improvement cosmetic composition ), Yolk containing IgY against Staphylococcus epidermidis , Micrococcus luteus and Actinomyces israelii .

Egg yolk antibodies are a form of passive immunization using the bird's immune system. In the case of sub-avian oval animals, the immune antibodies obtained by active chickens from the mother chicken are transferred to egg yolk and the immune function is transmitted to the offspring. The largest proportion of antibodies in egg yolk is IgY, similar to IgG in the blood. Its molecular weight is about 200-220kDa, which is larger than mammalian IgG and has advantages over mammalian antibodies.

The present invention to produce egg yolk containing the antibody IgY against the acne bacteria Propionibacterium acnes ), Propionibacterium Propionibacterium propionicus , Staphylococcus aureus , Staphylococcus epidermidis , Micrococcus luteus luteus ) and Actinomyces israelii incubated in actinomyces , and then the vaccines were administered to the laying hens at three intervals at regular intervals. After inoculation, the antibody titer was the highest titer from 4 weeks after the 3rd inoculation, and after the inoculation at 10 weeks when the antibody titer began to decrease, the eggs with high antibody titers up to 5-14 weeks Only egg yolk was collected and obtained.

On the other hand, the present invention is any one or more of the first extract selected from Yeongyo extract, three hundred seconds extract or lettuce extract; At least one secondary extract selected from agrimoni extract or spectral extract; Any one or more tertiary extracts selected from dandelion extract, cotyledon extract, rice extract or bamboo extract; And a water-soluble mixture of a mixture of purified water; such extracts are components used in the cosmetic composition for improving acne as well as showing an excellent effect on the prevention of acne, and can reduce the consumer's rejection with natural preparations. . The extract may be a hot water extract or an aqueous ethanol extract.

On the other hand, if you look at the development of acne, the production of sebum in the pores is increased, the skin epithelial cells are abnormally keratinized to block the opening of the pores, where acne-causing bacteria proliferate and cause inflammation.

Inflammatory responses are also induced by a wide range of external and internal stimuli, including lipopolysaccharide (LPS), tumor necrosis factor (TNF) -α, interleukin (IL) -1, It is a mechanism to regenerate damaged tissues, but management of inflammation is important because sustained inflammatory reactions promote mucosal damage, and the damaged tissues may develop into cancer.

In this experimental example, TPA (12-O-tetradecanoylphobol 13-acetate), which is known as a potent carcinogen, is used to confirm the anti-inflammatory effect of the present invention on inflammation caused by acne-causing bacteria and other causes. After inducing edema by contacting the ears of the mouse, the anti-inflammatory effect was confirmed by treating the acne improvement cosmetic composition of the present invention. Experimental results showed that TPA treatment suppressed the increase of ear thickness and weight, decreased the contents of TNF-α and IL-1, which induce inflammatory reactions, and peroxidase in granules of eosinophils and neutrophils as an indicator of inflammation. It was confirmed that phosphorus MPO activity was greatly suppressed. In addition, as a result of histological analysis of the anti-inflammatory effect of the cosmetic composition for improving acne of the present invention, it was confirmed that TPA treatment suppresses symptoms such as edema of the ear tissue, overgrowth of the epidermis and infiltration of inflammatory cells. .

On the other hand, the cosmetic composition for improving acne of the present invention is most preferably 0.10 to 5.00% by weight of yolk; 0.01 to 5.00 wt% of any one or more primary extracts selected from Yeongyo extract, Triticale extract, or Morus alba extract; 0.01 to 5.00 wt% of at least one secondary extract selected from agrimoni extract or crow root extract; 0.01 to 5.00% by weight of any one or more tertiary extracts selected from dandelion extract, cotyledon extract, rice extract or bamboo extract; And remaining distilled water.

As described above, the cosmetic composition for improving acne of the present invention can effectively prevent and improve acne by containing egg yolks having specific antibodies against various causative agents causing acne, as well as by using natural materials. It can reduce your rejection.

Hereinafter, the configuration and operation of the present invention will be described in more detail with reference to the following examples, but the scope of the present invention is not limited to the following examples, and includes modifications of equivalent technical spirit.

Example  One: Acne  Against antibodies IgY Containing Egg yolk  Produce

Example 1 Propionibacterium IgY for acnes), propynyl sludge tumefaciens propynyl sludge kusu (Propionibacterium for antibody IgY, Ste Philo aureus (Staphylococcus aureus) in propionicus) IgY, Ste Philo Caucus epidermidis (Staphylococcus Egg yolk containing IgY for epidermidis , IgY for Micrococcus luteus and IgY for Actinomyces israelii was prepared.

Stage 1: Antigen Production

Propionibacterium Acnes (KCTC 3320; Propionibacterium acnes ), Propionibacterium propionicus (KCTC 5342; Propionibacterium propionicus ) was anaerobicly cultured in blood incubator in a 37 ° C. incubator for 1-2 days. After confirming the colonies (colony), inoculated in the prepared BHI broth and incubated for 1 to 2 days in a 37 ℃ shaking incubator (shaking incubator). The obtained culture was inactivated with 0.3% formalin, then centrifuged at 10000 × g, and then inactivated with 0.1% formalin. The concentration was adjusted to 10 ¹² cells / ml using a Spectrophotometer.

Staphylococcus aureus (KCTC 1621; Staphylococcus aureus ), Staphylococcus epidermidis (KTCT 1917; Staphylococcus epidermidis ), Micrococcus luteus (KCTC 1056; Micrococcus luteus ), Actinomyces Israeli (KCTC 5559; Actinomyces israelii ) were incubated for 24 hours in a 37 ° C shaking incubator using BHI broth. The obtained culture was inactivated with 0.3% formalin, then centrifuged at 10000 × g and then inactivated again with 0.1% formalin. The concentration was adjusted to 10 ¹² cells / ml using a Spectrophotometer.

Step 2: Vaccine Preparation

After mixing the strain cultured in step 1 and mineral oil (ISA 70) in a 3: 7 ratio, and then emulsified using a homogenizer (Hmogenizer), the aseptic test and inactivation confirmation test.

Step 3: Antibodies Specific to Acne Bacteria Egg yolk  production

The vaccine prepared in step 2 was inoculated into the chest at 2 weeks intervals by 1 ml in a 22-week-old Hy-Line Brown laying hen, followed by 'Boosting' after 6 weeks after the third inoculation of antibody titers.

The titer of yolk yolk was measured by 'Indirect ELISA Method', and the measurement samples were measured weekly by collecting yolk from 1 week after inoculation to 2 weeks after booster vaccination. The yolk antibody activity was measured by applying the ELISA method used by Mine (1997).

Antigen was coated at a concentration of 200 ng / ml in 96 well polystyrene plate (well poly styrene plate) and left overnight at 4 ℃. After washing with PBS-T (phosphate buffer saline, 0.05% Tween 20, pH 7.4), 1h / 37 ℃ blocking with PBS buffer containing 2% BSA (Blocking) and washed in the same manner as described above. The negative control group, the positive control group and the sample were diluted by 2 ×, and 100 µl of each well was added and allowed to stand at 37 ° C. for 1 hour. The appropriately diluted secondary antibody was reacted in the same manner as the sample treatment and developed for 10 minutes, and then the absorbance was measured at 492 mm using an ELISA Reader.

The titer of the IgY antibody titers of eggs obtained from hens immunized with acne-causing bacteria was measured by ELISA (Fig. 1), and the IgY antibody titers of eggs began to increase after 3 weeks of immunization, the highest after 4 weeks after the third inoculation. The titer was shown. Additional vaccination was given at 10 weeks after the IgY antibody titer began to decline, and afterwards, the titer of IgY began to increase, and from 14 weeks, IgY titer gradually decreased.

Step 4: Egg yolk powder  Produce

Eggs collected from 5 to 14 weeks with IgY antibody titer greater than 160X of the negative control were concentrated, and only egg yolks were harvested after freezing and lyophilized to produce egg yolk powder.

Example  2: ampoule manufacturing

In Example 2, an ampoule was prepared using the specific antibody yolk powder obtained in Example 1 above.

Specific antibody yolk powder 20g; 1 g of Yeongyo extract, 1 g of three hundred sec extract or 1 g of lettuce extract; 5 g of agrimoni extract, 5 g of crow root extract; 2 g of dandelion extract, 2 g of self-leaving extract, 2 g of rice extract, 2 g of bamboo extract; And the remainder was added purified water to prepare 1000g of ampoules.

Experimental Example  One: Acne  Against antibodies IgY Containing Egg yolk  The effectiveness of the test and ampoules

A mouse ear edema model induced by TPA (12-O-tetradecanoylphorbol 13-acetate) was performed to verify the anti-inflammatory effects of acne-specific antibody yolk and ampoule.

This test is to evaluate the anti-inflammatory effect of these test substances by repeatedly applying acne bacteria-specific antibody yolk and ampoule to the mouse ear edema model by repeated application of TPA. After repeated application of TPA and test substance to the mice, ear thickness, ear weight, pro-inflammatory cytokine in the tissue and myeloperoxidase (MPO) The anti-inflammatory effect of the test substance was evaluated by activity and histological examination.

Egg yolk powder (hereinafter referred to as 'specific antibody yolk powder' or 'specific antibody yolk') containing the specific antibody against acne bacteria obtained in Preparation Example 1 in 20, 50, 100 mg / ml in sterile tertiary distilled water After concentration, 50 ml / ear was applied to the mouse ears. Final doses applied to mouse ears were 1, 2.5, 5 mg / ear.

Ampoules were treated in 50 ml / ear increments to allow ampoule stock to be applied sufficiently to ear tissue without dilution.

 Anti-inflammatory drug dexamethasone (dexamethasone) was used as a positive control, dexamethasone (dexamethasone) was dissolved in 70% ethanol to make 1mg / ml, and then applied to the mouse ears by 50 ml / ear. The final dose applied to mouse ears was 0.05 mg / ear.

  The ICR mice used in the experiment were 34 females (20-22 g) 5 weeks old and 34 females (23-25 g) 6-week old females purchased from Orient Bio Co., Ltd. The ear was marked according to the individual identification method. General symptoms were observed once daily during the 7-day quarantine purifying period, and healthy animals were selected by weighing with small animal scales (BP3100S, Sartorius, Germany) at the time of import and at the end of the quarantine purifying period. .

     At the end of the quarantine period, animals without weight gain and general symptoms were selected, and the animals were placed equally among the groups by calculating the average weight and standard deviation, and the animals were euthanized to separate the groups. After the group separation, the breeding box was attached to the individual identification card, individual identification was marked on the ear according to the individual identification method using a punch.

2 ~ 3 animals were housed in polycarbonate breeding box (278W * 420D * 200H (mm), 3 sign), temperature 20.9 ~ 22.6 ℃, humidity (relative humidity) 39.5 ~ 66.7%, ventilation times and methods 10 ~ 15 times 12 hours lighting / 12 hours off (7 AM ~ 7 PM lighting), illumination was maintained at 150 ~ 300 Lux.

      Breeding box was once / week, animal drinking water was exchanged every day, and washing was done by using general detergent, and it was sterilized in microquat (main ingredient: Alkyldimethylbenzyl ammonium chloride 400 times, ECOLAB Co., Ltd.) diluent and autoclave It was sterilized using.

     Disinfection of the animal room was performed after the end of the work every day, the disinfectant solution was used daily microquat (400 times the main ingredient: Alkyldimethylbenzyl ammonium chloride, ECOLAB Co., Ltd.).

The feed was supplied with sterile solid feed for Harlan experimental animals, and fed to the feeder and fed free. Drinking water was purified water filtered and sterilized using a filter and an oil sterilizer, and purified water was added to a polycarbonate drinking bottle (250 ml).

The test group was configured as shown in Table 1 below.

group TPA Test substance concentration Throughput Coating capacity Number of animals 2.5 mg / 20 ml / ear (mg / ml) (ml) (mg / ear) Control
(control) Control group Acetone Distilled water 50 - 3 TPA treatment group Vehicle ○ Distilled water 50 - 6 1 mg / ear specific antibody yolk powder ○ 20 50 One 5 2.5 mg / ear specific antibody yolk powder ○ 50 50 2.5 5 5 mg / ear specific antibody yolk powder ○ 100 50 5 5 Ampoule ○ Ampoule 50 - 5 Dexamethasone ○ One 50 0.05 5

The route of administration was the final application method of the test substance used in this test, so it was directly applied to the ear tissue by dose, and the dose of each group was set according to the concentration of the test substance product. The test substance dose was calculated and then diluted and diluted in tertiary distilled water, and a new one was made daily and applied to the ear tissue.

TPA and dexamethasone were purchased from Sigma Chemical Co. (St Louis, MO, USA) and used for testing.

In the ear edema model by TPA, TPA (2.5 mg / 20 ml acetone / ear) was applied to the ears of the mouse for 6 times, once every two days inside and outside the ear, and the control group was treated with 20 ml acetone / ear. Test substance and dexamethasone were applied continuously for 10 days at 50 ml / ear at the same time every day from the day after the first TPA treatment. On the day when TPA and test substance were to be treated together, the test substance was applied 30 minutes after TPA treatment and completely absorbed by the tissue. Ear thickness was measured 6 hours after the last TPA treatment, ear tissues were obtained and used for analysis (FIG. 2).

The results were expressed as 'means ± SEM' and statistical significance of the data was tested by 'ANOVA-test' using 'GraphPad Prism 4.0 software (GraphPad Software Inc., San Diego, CA, USA)'. .

Experimental Example  1-1: Weighing

Body weight was measured before the start of the experiment and 10 days before the dissection to calculate as shown in Equation 1.

% Weight gain = (weight on day 10 / weight before start of experiment) × 100

As a result of measuring the weight (FIG. 3), the dexamethasone group lost weight during the observation period, but there was no significant difference between the groups, and the weight change of the control group and the test substance administration group was normal.

Experimental Example  1-2: Ear thickness and ear weight measurement

 In Experimental Example 1-2, ear thickness and ear weight were measured for each experimental group.

Ear thickness was measured 6 hours after the last TPA treatment using a micrometer, ear weight was measured at 5 mm ² diameter ear punch after sacrificing the animal, and then precision scales (Mettlertoledo LTD., Seoul, Korea).

These test substances were induced by sensitizing six times with TPA in the mouse's ear to induce an inflammatory response. As a result of analyzing the edema suppression effect of TPA (FIGS. 4 and 5), the thickness and weight of the ears were significantly increased in the 0 mg group of egg yolk treated with TPA compared to the control group treated with acetone only (acetone) ( p <0.001). The experimental group coated with acne-specific antibody yolk and ampoule significantly inhibited the increase of ear thickness and weight.05, p <0.01, p <0.001), especially 5 mg / ear-specific antibody yolk The group was found to inhibit the increase in the thickness and weight of the ear at the level of dexamethasone (0.05 mg / ear), an inflammation treatment agent.

Experimental Example  1-3: In Your Tissue Proinflammatory Cytokines ( pro - inflammatory cytokines Content measurement

In Experimental Examples 1-3, the content of pro-inflammatory cytokines in ear tissues was measured.

IL-6, a pro-inflammatory cytokine, is secreted by monocytes and macrophage and is known to increase in inflammation at all times.

TNF-a is a cytokine that plays an important role in the innate immune response as a major mediator of the LPS response and is known to increase in chronic inflammatory responses.

Ear tissue was obtained 6 hours after the last TPA treatment, and the tissue was homogenized with 50 mM Tris-HCl buffer (pH 7.5, 1 mM EDTA, 0.1% Triton X-100) corresponding to three times the tissue weight. After centrifugation at 10,000 × g at 4 ° C. for 10 minutes, the supernatant was obtained, and the concentration of IL-6 and TNF-a contained in the supernatant was determined using an 'enzyme-linked immunosorbent assay kit (eBIOSCIENCE, Sandiego, CA, USA)'. The analysis was carried out. The contents of IL-6 and TNF-a were corrected by ear tissue weight, and the results were expressed as relative to the control group (acetone treatment group).

As a result of the measurement (FIG. 6 and FIG. 7), the IL-6 content in the ear tissue was increased in the TPA-only group than the control group, but there was no significant difference. The concentration of IL-6 in tissues was decreased in a concentration-dependent manner by the treatment of specific antibody yolk, and the inhibitory effect was greatest in the group treated with 5 mg / ear of egg yolk. It was confirmed that the production of IL-6 by TPA was also suppressed by the application of ampoule. Their level of inhibition was similar to that of the dexamethasone treatment group, but there was no significant difference between the groups.

  In the edema model by TPA treatment, TNF-a, unlike IL-6, showed a high level in the control group, and the TNF-a content was decreased by TPA treatment. The concentration of TNF-a was increased by treatment with specific antibody yolk, but there was no significant difference compared with the control or specific antibody yolk 0mg group.

Experimental Example  1-4: In Your Organization Myeloperoxidase ( myeloperoxidase ; MPO ) activation Measure

In Experimental Example 1-4, myeloperoxidase (MPO) activity in ear tissues was observed. Measured. MPO is a peroxidase present in the granules of neutrophils and eosinophils.

MPO activity was assessed in your tissue 6 hours after the last TPA treatment. Homogenize with 50 mM potassium phosphate buffer (pH 6.0, 0.5% hexadecyltrimethylammonium bromide) corresponding to three times the tissue weight and centrifuged at 10,000 × g for 15 minutes at 4 ° C. Supernatant was obtained after separation. 50 ml of the supernatant, 1420 ml of potassium phosphate buffer, 15 ml of O-dianisidine dihydrocloride (20 mg / ml) and 15 ml of 20 mM H ₂ O ₂ , followed by 460 Absorbance was measured at nm, reacted at 37 ° C. for 3 minutes, and MPO activity was evaluated using the difference in absorbance. Ear tissue weights were corrected and expressed as relative to control.

As a result of measurement (Fig. 8), MPO activity was significantly increased by repeated application of TPA compared to acetone-only control group ( p <0.01, p <0.001), and 5 mg / ear acne-specific antibody yolk treated group It was confirmed that the MPA activity induced by TPA was most effectively inhibited.

Experimental Example  1-5: Histological Examination of Your Tissue

In Experimental Example 1-5, histological examination of the ear tissue was performed.

Six hours after the last TPA treatment, your tissues were excised to 'O.C.T. compound (Sakura Finetechnical Co., LTD., Tokyo, Japan), frozen, and cut into 8 mm thick tissue sections using 'Cryostat (Thermo SCIENTIFIC, Waldorf, Germany)' and stained with 'hematoxylin & eosin'. We evaluated histological changes due to inflammatory responses induced by TPA.

As a result of histological examination of the inflammation inhibitory effect of acne bacteria-specific antibody yolk and ampoule in the ear edema model induced by TPA (FIG. 9), the repeated application of TPA to mouse ear tissues showed edema, epidermal hyperplasia, and inflammatory cells. Symptoms such as invasion of The experimental group coated with specific antibody yolk against acne bacterium effectively inhibited TPA-induced edema, epidermal hyperplasia and infiltration of inflammatory cells, especially in the 5 mg / ear-specific antibody yolk group. The level was similar to the group treated with dexamethasone, an inflammatory agent.

Example  3: Ampoule  Cosmetic lotion containing

To 8 g of 95% ethanol, 0.05 g of polypyrrolidone, 0.1 g of oleyl alcohol, 0.2 g of polyoxyethylene monooleate, 0.2 g of fragrance, 0.1 g of paraoxybenzoic acid methyl ester, a small amount of antioxidant, and a small amount of pigment were dissolved. . An ampoule was prepared by adding 0.05 g of the ampoule obtained in Example 2 and 5 g of glycerine to 85.33 g of purified water, followed by stirring the mixed solution.

Example  4: Ampoule  Latex preparation

1.2 g of cetyl alcohol, 10 g of squalane, 2 g of vaseline, 0.2 g of paraoxybenzoic acid ethyl ester, 1 g of glycerin monoesteralide, 1 g of polyoxyethylene (20 mole added) monooleate and 0.1 g of fragrance were mixed and dissolved at 70 ° C. 0.5 g of the ampoule obtained in Example 2, 5 g of dipropylene glycol, 2 g of polyethylene glycol-1500, 0.2 g of triethanolamine, and 76.2 g of purified water were heated to dissolve at 75 ° C. Both were emulsified by mixing, followed by cooling to prepare an oil-in-water (O / W) type emulsion.

Example  5: Ampoule  Containing Essence  Produce

5 g of 95% ethyl alcohol, 1.2 g of polyoxyethylene sorbitan monooleate, 0.3 g of kitulose, 0.2 g of sodium hyaluronate, 0.2 g of vitamin E-acetate, 0.2 g of sodium licorice, 0.1 g of paraoxybenzoic acid ethyl ester, 1g of ampoules obtained in Example 2 and an appropriate amount of pigments were mixed to prepare a cosmetic solution.

1 is a diagram measuring the IgY antibody titer of egg yolk obtained from laying hens immunized with acne-inducing bacteria by ELISA.

FIG. 2 shows egg yolk (1, 2.5, 5 mg / ear), ampoule (Ampoule; 50 ml / ear), dexamethasone (0.05 mg / ear) with antibodies against acne bacteria 30 minutes after TPA treatment for 10 days ), After repeated treatments, the mice were sacrificed and 6 hours after the last treatment with TPA on the 10th day of the mice.

FIG. 3 is a diagram measuring body weight change before dissecting the mouse ear edema model induced by TPA as shown in FIG. 2 on the 10th day.

4 is a diagram measuring the thickness of the ear by the specific antibody yolk, ampoule, dexamethasone treatment for the mouse ear edema model induced by TPA as shown in the experimental design shown in FIG.

FIG. 5 is a diagram measuring ear weight change caused by specific antibody yolk, ampoule, and dexamethasone treatment for a mouse ear edema model induced by TPA as shown in FIG. 2.

6 is a measurement of the inhibitory effect of pro-inflammatory cytokines by the treatment of specific antibody yolk, ampoule, and dexamethasone in the mouse ear edema model induced by TPA as shown in FIG. Road, a diagram showing the content of IL-6, a pro-inflammatory cytokines.

FIG. 7 measures the inhibitory effect of pro-inflammatory cytokines by specific antibody yolk, ampoule, and dexamethasone treatment on a mouse ear edema model induced by TPA as shown in FIG. Fig. Shows the content of TNF-α, a pro-inflammatory cytokines.

FIG. 8 is a diagram measuring the effect of inhibiting MPO activity by specific antibody yolk, ampoule, and dexamethasone treatment in a mouse ear edema model induced by TPA as shown in FIG. 2.

FIG. 9 is a hematoxylin and eosin for histopathological examination of a mouse ear edema model induced by TPA as shown in FIG. Measured by staining with '(original magnification, x100).

Claims (2)

IgY for Propionibacterium acnes , IgY for Propionibacterium propionicus , IgY for Propionibacterium propionicus , Staphylococcus aureus , Staphylococcus epidermidis Egg yolk containing IgY for Staphylococcus epidermidis , IgY for Micrococcus luteus and IgY for Actinomyces israelii ; At least one primary extract selected from pontoon extract, triticale extract or lettuce extract; At least one secondary extract selected from agrimoni extract or spectral extract; Any one or more tertiary extracts selected from dandelion extract, cotyledon extract, rice extract or bamboo extract; And purified water; Cosmetic composition for acne improvement comprising a water-soluble mixture of the mixture. The method of claim 1, Cosmetic composition for acne improvement, Egg yolk 0.10 to 5.00 wt%; 0.01 to 5.00 wt% of any one or more primary extracts selected from Yeongyo extract, Triticale extract, or Morus alba extract; 0.01 to 5.00 wt% of at least one secondary extract selected from agrimoni extract or crow root extract; 0.01 to 5.00% by weight of any one or more tertiary extracts selected from dandelion extract, cotyledon extract, rice extract or bamboo extract; And cosmetic composition for improving acne, characterized in that the composition of the remaining distilled water.

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