KR101083426B1 - Antidiabetic composition containing the extract of Lycium chinense, Cordyceps sinensis and Acanthopanax senticosus Harms - Google Patents

Abstract

The present invention relates to an antidiabetic composition comprising a mixture of 1 to 6 parts by weight of a dry powder of prickly pear extract and a dry powder of Cordyceps sinensis extract with respect to 1 part by weight of a dry powder of a phalanx extract. Inhibits the activity of α-glucosidase while increasing the activity of glucokinase and pyruvate dehydrogenase and acetyl-CoA carboxylase. It is possible to exert substantial anti-diabetic effect and to properly limit the blending ratio of the materials to provide an economical and effective anti-diabetic composition.

Spinocopi, Glucokinase, Diabetes, Cordyceps Sinensis, Acetyl-Coei Carboxylase, Alpha-Glucosidase, Composition, Phalanx, Pyruvate Dehydrogenase, Blood Sugar

Description

Antidiabetic composition containing the extract of Lycium chinense, Cordyceps sinensis and Acanthopanax senticosus Harms

The present invention relates to an anti-diabetic composition, and more particularly, to an anti-diabetic composition containing the extract of phalanges, cordyceps, thorny stems.

Diabetes is a chronic disease in which glucose is released into the urine due to higher glucose (blood sugar) levels in the blood due to a lack of insulin, and the lack of insulin secreted by B cells of the island of Langerhans in the pancreas, anterior pituitary gland, adrenal gland Endocrine glands, such as the thyroid gland, and the central nervous system, especially the hypothalamus, such as the cause of the cause, combined with these factors are thought to occur.

Genetic predisposition is important as a cause of diabetes. Especially in children and young patients, most of them may be related to heredity, and diet is closely related to the onset. It is estimated that total calorie, high protein, and high fat diet are related, and the relationship with obesity is attracting attention because it is almost one-half to half of patients.

Early major symptoms of diabetes include polyuria, thirst, drowsiness, multi-diet, and weight loss, and long-term hyperglycemia is associated with cardiovascular disease, retinopathy (loss of vision), nephropathy (renal failure), and / or Causes neuropathy (foot ulcers, amputations, etc.).

According to a 2002 Center for Disease Control and Prevention (CDC) survey, 6.3% of Americans have diabetes (21.1% of the adult population) and about 1.3 million new cases (over 20 years old). ) Is reported annually, which is estimated to be diagnosed in only about half of people with diabetes.

In order to prevent or treat the above diabetes, insulin or oral hypoglycemic agents are administered, but since they are accompanied by economic burden and risk of side effects, in recent years, diet and natural therapy using pharmacological substances such as natural plants are used. This is being tried.

However, many of them lack the scientific evidence and question whether the anti-diabetic effect is practical, and the fact that they do not calculate the specific compounding ratio of the added materials is not economical and efficient.

Therefore, the present invention aims to develop and provide an antidiabetic composition exhibiting economical and practical antidiabetic effect by finding out how the materials exert antidiabetic effect through a stepwise experiment and limiting an appropriate blending ratio of the materials. .

In order to achieve the above object, the present invention is characterized by containing a mixture of 1 to 6 parts by weight of the dry powder of prickly thorn extract and dried powder of Cordyceps sinensis extract with respect to 1 part by weight of the dry powder of the Gigolpi extract as an active ingredient. It provides an antidiabetic composition.

Hereinafter, the problem solving means of the present invention will be described in detail.

Lycium chinense is a goji tree root bark, which is rich in unsaturated fatty acids and amino acids such as betaine, rutin, gucoamine A, betacicetrol, etc. in addition to general and inorganic components. Inorganic ingredients include potassium, magnesium, calcium, iron and zinc. Phalanges blood fever cools the blood to cool the fever (淸 熱 凉血) action and lung lung heat fever to cool the lungs (淸 肺熱) action, due to lung fever, sea water, asthma, hemostasis, nosebleeds, urine hemorrhage, It is effective for osteopathic fever (consumable disease such as tuberculosis).

Cordyceps sinensis ) is a type of medicinal mushroom that forms a fruiting body on the epidermis of insects after some time when the fungus Cordyceps fungi enters the body of living insects at the time of high temperature and humidity. The pharmacological action of Cordyceps sinensis has been reported in the central nervous system, temperature lowering, immune function, anti-cancer, increased cardiac blood flow, bronchodilation, increased adrenal cortex hormone, anti-fatigue, anti-aging, anti-inflammatory, anti-microbial action.

Acanthopanax senticosus Harms, Eleutherocosis maxim ) is a perennial deciduous shrub belonging to the Araliaceae, which grows in the highlands of the Korean Peninsula, Siberia and China. Its main components are seven types of triterpenoid glycosides, ereuterosides A, B, C, D, E, F, and G. Other sugars such as glucose and galactose, carotene, vitamin B ₁ and B ₂ It is rich in minerals, C and minerals. Also known as “Siberian ginseng”, thorny ginseng is a tonic, bronchial asthma, treatment, stamina, musculoskeletal enhancement, anti-cancer, anti-aging, anti-fatigue, and metabolism that is comparable to ginseng. It is known.

On the other hand, although the anti-diabetic composition comprising the extract of the phalanx, the extract of Cordyceps sinensis and the extract of thorny ogapi has already been produced, the scientific basis for how the anti-diabetic effect is exerted through the action of each material is weak, and the combination ratio of the materials It is true that economics and efficiency fall because it is not taken into account. In order to solve such a problem, the present invention has been conducted through a step-by-step experiment to find out what the dry powder of the phalanx and Cordyceps Sinensis and Prickly Pear extract acts on the diabetes-induced rats. Develop and provide a composition.

On the other hand, diabetes is a chronic disease in which glucose is released into the urine due to a high level of glucose (blood glucose) in the blood due to lack of insulin, and in the present invention, to develop and provide an antidiabetic composition for preventing and treating the same, cells by material Glycokinase and pyruvate dehydrogenase, enzymes involved in breaking down sugars and using them as substrates for energy metabolism, generate energy and convert surplus sugar into fat. Increasing activity of the enzyme acetyl-CoA carboxylase (Acetyl-CoA carboxylase) and inhibition of the activity of α-glucosidase (α-glucosidase) was measured.

The enzymes described in more detail, glucokinase (glucokinase) is the first step involved in the metabolism of intracellular sugar, and when sugar enters the cell, it breaks down the sugar and decreases the intracellular sugar concentration. When the concentration is lowered, sugar enters from the blood and eventually lowers blood sugar.

Pyruvate dehydrogenase is an enzyme that irreversibly converts pyruvate, which is produced during the decomposition of intracellular sugars by glucokinase and the like, into acetyl CoA. Pyruvate dehydrogenase-treated sugar metabolism can reduce the intracellular sugar concentration because it is impossible to produce reversible sugar again. When the intracellular glucose concentration decreases, blood sugar enters the cell and eventually lowers the blood sugar. .

Acetyl-CoA carboxylase is an enzyme that limits the rate of fatty acid biosynthesis in animal tissues. In the cell, sugar produces energy through the TCA cycle, converting the surplus of sugar into fat. It is an enzyme involved in making.

α-glucosidase is an enzyme that breaks down carbohydrates and converts them into glucose, and is an enzyme that contributes significantly to post-prandial blood sugar rise. Therefore, by inhibiting the activity of α-glucosidase, the post-prandial carbohydrate digestion and absorption is delayed, thereby significantly reducing blood sugar and reducing insulin levels in the blood, thereby gradually reducing the fasting blood sugar. .

As a result of measuring the activity of the enzymes by preparing dried powders of the phalanges, thorns, and Cordyceps sinensis extracts by concentration, the activity of glucokinase, pyruvate dehydrogenase, and acetyl-coei carboxylase was increased. In particular, the zigolpi extract significantly increased the activity of glucokinase, the pterygium extract increased the activity of pyruvate dehydrogenase, and the cordyceps sinensis significantly increased the activity of acetyl-Coei carboxylase. The activity of glucosidase was the most inhibited by prickly pear extract.

On the other hand, the anti-diabetic composition of the present invention is characterized by containing a mixture of 1 to 6 parts by weight of the dry powder of prickly pear skin extract and the dry powder of Cordyceps sinensis extract with respect to 1 part by weight of the dry powder of the phalanx extract as an active ingredient. When less than 1 part by weight of the dry powder of prickly pear extract is contained per 1 part by weight of the dry powder of the extract, not only is it difficult to increase the activity of pyruvate dehydrogenase, but also it is difficult to inhibit the activity of α-glucosidase. , If the dry powder of the thorn extract is more than 6 parts by weight, economic efficiency is inferior to the material input amount. It is difficult to increase the activity of acetyl-Coei carboxylase when the dry powder of Cordyceps sinensis extract is contained less than 1 part by weight with respect to 1 part by weight of the phalanx extract, and when the dry powder of Cordyceps sinensis extract exceeds 6 parts by weight Contrast economy is low.

On the other hand, the dry powder of the phalanx extract or dry powder of prickly pear extract or dry powder of Cordyceps sinensis extract is not limited to a specific method, preferably 1 weight part of any one dry sample selected from the phalanges, psyllium stem and cordyceps. Filtrate obtained by adding 8.7 to 12.7 parts by weight of distilled water and shaking incubation at 50 to 70 ° C. for 20 to 28 hours, centrifugation at 5000 to 7000 rpm for 50 to 70 minutes, and filtering to 0.35 to 0.55 μm. It is preferably prepared by lyophilization.

On the other hand, the anti-diabetic composition of the present invention preferably contains a mixture of 3 parts by weight of the dry powder of prickly thorn extract and dried powder of Cordyceps sinensis extract with respect to 1 part by weight of the dry powder of the phalanx extract as an active ingredient, gluco This is because the kinase, pyruvate dehydrogenase, and acetyl-CoA carboxylase have the most excellent effect of increasing the activity, and the α-glucosidase has an excellent activity inhibitory effect.

As described above, the anti-diabetic composition of the present invention is α-glucose while increasing the activity of glucokinase, pyruvate dehydrogenase, and acetyl-CoA carboxylase. The activity of the α-glucosidase exerts a substantial anti-diabetic effect that inhibits and is effective and economical by appropriately limiting the compounding ratio of the materials.

In addition, the present invention is derived from a natural material has the advantage of not rejection and discomfort from the general consumer.

Hereinafter, the configuration and operation of the present invention will be described in more detail with reference to the following examples, but the scope of the present invention is not limited to the following examples, and includes modifications of equivalent technical spirit.

< Manufacturing example  1> Sample Preparation

After adding 214 ml of distilled water to 20 g of each of the phalanges, Cordyceps sinensis and Prickly Pear, each was incubated at 60 ° C for 24 hours, and then centrifuged at 6,000 rpm for 1 hour. The extract obtained by centrifugation was filtered to 0.45 mu m and then lyophilized to obtain each powder. Each of the powders obtained was mixed with distilled water to be 50 ppm, 100 ppm, 250 ppm, 500 ppm, 1000 ppm, 5000 ppm, 10000 ppm, thereby preparing an extract sample.

< Experimental Example  1> Phalanges Antidiabetic Effects of Dried, Cordyceps Sinensis and Prickly Pear Extracts on Dry Powder

Glucokinase and pyruvate dehydrogenase and α-glucosidase, which are enzymes involved in glucose utilization metabolism by each of the samples of the phalanges, Cordyceps sinensis and Prickly Ophitus extracts obtained in Preparation Example 1 α-glucosidase) and acetyl-CoA carboxylase (Meetyl-CoA carboxylase) activity was measured to determine the anti-diabetic effect through the action.

 Cell fractions were prepared by collecting cardiac tissue and liver tissue from GK (Goto-Kakizaki), a type 2 diabetes-induced rat, which was established by selective mating as an indicator of poor glucose tolerance in Wister rats by oral glucose tolerance test. The activity of the enzymes present was measured on the extract samples of phalanges, Cordyceps sinensis, Prickly Pear.

At this time, the cell fraction is collected Cardiac and hepatic tissues of GK, a type 2 diabetes-induced rat (Ref .: Biochemistry, Torturer, Kang Yun-se et al., 1996, 1996). Heart tissue was harvested after cutting the artery connected to the heart and removing blood.

< Experimental Example  1-1> Glucokinase ( Glucokinase Investigation of the degree of activity regulation

Glucokinase is the first enzyme involved in intracellular glucose metabolism. Glucokinase lowers glucose levels in the blood and lowers blood sugar by lowering glucose levels in the cells. Therefore, this Experimental Example 1-1 was carried out to determine whether the extract samples of phalanges, Cordyceps sinensis, Prickly Ogapi obtained in Preparation Example 1 increases the activity of glucokinase.

Glucokinase Activity of Extracts from Cordyceps, Cordyceps Sinensis, and Prickly Pear Extract The degree of control was measured from hepatic cell fractions of GK, type 2 diabetes induced mice.

The measurement method was incubated at 28 ° C. by adding 44 mM sodium glycylglycinate (pH 7.5), 3 mM NADP ⁺ , 3 mM MgCl ₂ , 30 mM ATP, 0.2 U G-6-PDH, 500 mM glucose (glucose). After incubation), each extract sample and cytosol were added, and then measured at 340 nm for 3 minutes to measure the activity of glucokinase through oxidation of NADPH.

As a result of measuring the activity of glucokinase (in% of the control group) (FIG. 1), all samples of the phalanx, Cordyceps sinensis and prickly pear extracts increased the activity of glucokinase. The activity increased sharply and reached a maximum at 10000 ppm.

< Experimental Example  1-2> Pyruvate Dehydrogenase ( pyruvate dehydrogenase ) Investigation of the degree of active regulation

Pyruvate dehydrogenase is an enzyme that irreversibly converts pyruvate, which is produced during the breakdown of intracellular sugars by enzymes such as glucokinase, to acetylcoei. At this time, since the reversible production of sugar is impossible, the intracellular sugar is lowered, and when the intracellular sugar is lowered, blood sugar is introduced into the cell, thereby lowering the blood sugar.

 Therefore, in Experimental Example 1-2, it was confirmed whether the extract samples of phalanges, Cordyceps sinensis, and Prickly Pear increased the pyruvate dehydrogenase activity.

Activity of pyruvate dehydrogenase by extract samples of phalanx, Cordyceps sinensis and Prickly Pear The degree of control was measured from cardiac tissue fractions of GK, type 2 diabetes-induced rats.

The measuring method was measured according to the method of Flora H. et al. 150 mM MOPS (pH 7.4), 12 mM MgCl ₂ , 0.6 mM CaCl _{2 ,} 20 mM NAD ⁺ , 0.72 mM CoA, 18 mM thiamine pyrophosphate, 15.6 mM L-cysteine, 75 mM pyruvate After incubation at 30 ° C. for 3 minutes, each extract and pyruvate dehydrogenase were added and measured at 340 nm for 2 minutes to determine pyruvate dehydrogenase (oxidation of NADH). The activity of pyruvate dehydrogenase was measured.

As a result of measuring the activity of pyruvate dehydrogenase (expressed in% of the control group) (FIG. 2), all of the phalanges, cordyceps, and bark extracts increased the activity of pyruvate dehydrogenase, in particular, The sample showed high activity of pyruvate dehydrogenase even at low concentrations.

< Experimental Example  1-3> Acetyl- Koei Carboxylase ( Acetyl-CoA carboxylase ) Investigation of the degree of active regulation

Acetyl-CoA carboxylase is an enzyme that limits the rate of fatty acid biosynthesis in animal tissues, and is involved in the production of energy through the 'TCA cycle' in cells and the conversion of excess sugar to fat. do. Therefore, Experimental Example 1-3 was carried out to determine whether each of the extract samples of phalanges, Cordyceps sinensis, Prickly Ogapi increases the activity of acetyl-Coei carboxylase.

Acetyl-CoA Carboxylase Activity by Extract Samples of Phalanx, Cordyceps Sinensis, Prickly Pear The degree of control was measured from hepatic cell fractions of GK, type 2 diabetes induced mice.

Acetyl-CoA carboxylase activity was measured using the 'spectrophotometric' method such as 'Tadashi' to determine the glycemic control function of each extract samples of the phalanx, Cordyceps sinensis, thorn stem. 250 mM Tris-HCl (pH 7.5), 50 mM MgCl ₂ , 18.75 mM glutathione, 0.625 mM pH 8.0 NADH, 0.625 mM acetyl-CoA, 2.5 mM potassium phosphoenolpyruvate, 10 U lactate di Hydrogenase (lactate dehydrogenase), 10U pyruvate kinase, each extract, and cytosol were added and incubated at 37 ° C for 1 minute, then 37.5 mM ATP and 250 mM KHCO ₃ were added. 2 minutes at 340 nm, NADH was reduced to NAD ⁺ to determine the activity of acetyl-CoA carboxylase (acetyl-CoA carboxylase).

As a result of measuring the increase of acetyl-Coei carboxylase activity (expressed in% of the control group) (FIG. 3), the acetyl-Coei carboxylase activity was increased by the respective extract samples of the phalanges, Cordyceps sinensis, P. In particular, the extract sample of Cordyceps sinensis was confirmed to increase the concentration-dependent activity of acetyl-Coei carboxylase.

< Experimental Example  1-4> α- Glucosidase (α- glucosidase ) Investigation of the degree of active regulation

α-glucosidase is an enzyme that contributes to the post-prandial blood sugar increase by responsible for the digestion of carbohydrates. A material capable of inhibiting the activity of α-glucosidase inhibits the digestion and absorption of glucose by post-prandial blood sugar Sudden rise can be suppressed. Therefore, Experimental Example 1-4 was carried out to select a material that inhibits the activity of α-glucosidase.

Activity inhibition measurement of α-glucosidase is in Inhibition rate was measured by kinetic analysis method with substrate in vitro to determine the glycemic control function of each extract of phalanx, Cordyceps sinensis, Prickly Pear. 'Synthetic substrate' 2.5mM p-nitrophenyl α-D-glucospyranoside (p-nitrophenyl α-D-glucospyranoside) was added to the phosphate buffer (pH 6.8), and then α-glucosidase ( Each extract sample to be subjected to the α-glucosidase activity inhibition experiment was put, and an enzyme solution was added to the mixed solution. After the addition of the enzyme solution, the reaction was completed at 37 ° C. for 20 minutes and the reaction was terminated by adding 0.1 M NaOH to spectrometer (p-nitrophenol), which is a reaction product released from the substrate pNPG. The degree of inhibition of α-glucosidase activity was confirmed by measuring at 405 nm with a spectrometer).

As a result of measuring the degree of inhibition of activity of the α-glucosidase (expressed in% of the control group) (FIG. 4), it was confirmed that the degree of inhibition of activity of the α-glucosidase by the barberry extract sample was high.

On the other hand, as a result of measuring the cell survival rate for each extract sample (Fig. 5), it was confirmed that the cell survival rate for each extract sample is excellent.

< Example  1 to Example  5> Anti-diabetic  Composition manufacturing

Experimental Example 1 confirmed the anti-diabetic effect of the extract samples of each of the phalanges, Cordyceps sinensis, prickly gingigar through the action, in Examples 1 to 5 mixed the extract samples of each phalanx, Cordyceps sinus The antidiabetic composition was prepared by extracting in the same manner as in Preparation Example 1.

Anti-diabetic composition was prepared by varying the blending ratio to determine the blending ratio that can effectively exhibit the anti-diabetic effect when mixing each extract sample, the anti-diabetic effect according to the blending ratio was confirmed in the following experimental example.

The concentration of each extract sample contained in the anti-diabetic composition was determined based on the activity control function of each enzyme and the results of cytotoxicity test. 100 ppm of the thorny oga extract extract to maximize the activity, 5,000 ppm of Cordyceps sinensis extract sample to maximize the activity of acetyl-Coei carboxynase was prepared.

Formulation ratio of each extract sample having the concentration as described above is shown in Table 1 below.

Antidiabetic
Composition Compounding ratio (weight ratio) Phalanx Extract Sample Prickly Pear Extract Sample Cordyceps Sinensis Extract Sample Example 1 One One One Example 2 One 2 2 Example 3 One 3 3 Example 4 One 5 5 Example 5 One 6 6

< Experimental Example  2> Glucokinase ( Glucokinase Measurement of the degree of active regulation

The degree of glucokinase activity control by the antidiabetic composition prepared in Examples 1 to 5 was measured by the same method as Experimental Example 1-1.

As a result of measuring the degree of activity regulation (expressed in% of the control group) of glucokinase (FIG. 6), all of Examples 1 to 5 increased the activity of glucokinase compared to the control group, and in particular, Example 3 was 206.5% As compared to the control group showed about 21 times higher activity, which indicates higher activity than when measuring the phalanges extract samples alone.

From the above results, it can be inferred that the antidiabetic composition of the present invention can prevent and treat diabetes by increasing the glucokinase activity involved in intracellular glucose metabolism.

< Experimental Example  3> Pyruvate Dehydrogenase ( pyruvate dehydrogenase ) Determination of the degree of active regulation

The degree of pyruvate dehydrogenase activity control by the antidiabetic composition prepared in Examples 1 to 5 was measured by the same method as Experimental Example 1-2.

As a result of measuring the degree of activity regulation of pyruvate dehydrogenase (expressed in% of the control group) (FIG. 7), Example 3 exhibited the highest activity of 167.9%, which was measured alone. Higher activity than that.

From the above results, the anti-diabetic composition of the present invention prevents diabetes by increasing the activity of pyruvate dehydrogenase, an enzyme that irreversibly converts pyruvate produced during the breakdown of intracellular sugar into acetylcoa and It could be deduced that it could be cured.

< Experimental Example  4> Acetyl Koei Carboxylase ( Acetyl - CoA carboxylase ) Investigation of the degree of active regulation

The degree of inhibition of acetyl-CoA carboxylase activity by the antidiabetic composition prepared in Examples 1 to 5 was measured by the same method as Experimental Example 1-3.

As a result of measuring the degree of activity regulation of acetyl-CoA carboxylase (FIG. 8), all of Examples 1 to 5 showed higher activity than the control group, in particular, Example 3 was 169.6% It was measured highest as. This shows higher activity than when the Cordyceps sinensis extract was measured alone.

From the above results, it can be inferred that the antidiabetic composition of the present invention can prevent and treat diabetes by increasing the activity of acetyl-Coei carboxylase.

< Experimental Example  5> α- Glucosidase (α- glucosidase ) Determination of Activity Inhibition

In order to measure the degree of inhibition of α-glucosidase activity by the anti-diabetic composition of Example 3 prepared in Examples 1 to 5 was carried out in the same manner as in Experimental Example 1-4.

As a result of measuring the degree of activity inhibition of α-glucosidase (FIG. 9), the activity inhibition rate of α-glucosidase of Example 3 was 23.3%, compared to acarbose. In this case, the inhibition rate was similar to that of Acarbose 500 ppm (23.8%).

From the above results, it can be inferred that the anti-diabetic composition of the present invention can prevent and treat diabetes by inhibiting the activity of α-glucosidase, an enzyme that is responsible for digesting carbohydrates and contributes significantly to postprandial blood sugar rise.

1 is a diagram showing the glucokinase activity by the concentration of each extract of Phalanx, Cordyceps sinensis, Prickly Ogapi in% of the control group.

2 is a diagram showing the pyruvate dehydrogenase activity by the concentration of each extract of the phalanx, Cordyceps sinensis, Prickly Ogapi as a percentage of the control group.

Figure 3 is a diagram showing the acetyl-Coei carboxylase activity in% of the control group by the extract concentration of phalanx, Cordyceps sinensis, Prickly Ogapi, respectively.

4 is a diagram showing the degree of inhibition of α-glucosidase activity by the concentration of each extract of Phalanx, Cordyceps sinensis, Prickly Ogapi in% of the control group.

Figure 5 is a diagram showing the cell survival rate according to the concentration of each extract of phalanx, Cordyceps sinensis, Prickly Ogapi.

Figure 6 is 10000ppm phalanx extract, 100ppm prickly gingival extract, 5000ppm Cordyceps sinensis extract 1 Example by weight of Pleurotussis extract and Cordyceps sinensis extract for 1 part by weight of Example 1, about 1 part by weight of phalanges extract Example 2 prepared by mixing 2 parts by weight of prickly pear skin extract and Cordyceps sinensis extract, Example 1 prepared by mixing 3 parts by weight of prickly pear skin extract and Cordyceps extract each 1 part by weight The glucokinase activity according to Example 5 prepared by mixing 5 parts by weight of the thorny gingival extract and Cordyceps sinensis extract, respectively, and 6 parts by weight of the thorny gingigar extract and Cordyceps sinensis extract, respectively, was added to the control group. The figure is expressed in%.

Figure 7 is 10000ppm phalanx extract, 100ppm prickly gingival extract, 5000ppm Cordyceps sinensis extract 1 Example by weight, respectively, 1 kg by weight of prickly pear skin extract and Cordyceps sinensis extract prepared by mixing 1 part by weight, respectively about 1 part by weight Example 2 prepared by mixing 2 parts by weight of prickly pear skin extract and Cordyceps sinensis extract, Example 1 prepared by mixing 3 parts by weight of prickly pear skin extract and Cordyceps extract each 1 part by weight Pyruvate dehydronase activity according to Example 4 prepared by mixing 5 parts by weight of the thorny ogapi extract and Cordyceps sinensis extract, respectively, and 6 parts by weight of the thorny thorn stem extract, Cordyceps sinensis extract, 1 part by weight Is the figure in% of the control.

Figure 8 is 10000ppm phalanx extract, 100ppm prickly gingival extract, 5000ppm Cordyceps sinensis extract for Example 1 prepared by mixing 1 kg by weight of psyllium extract and Cordyceps sinensis extract by 1 part by weight, respectively, about 1 part by weight of phalanges extract Example 2 prepared by mixing 2 parts by weight of prickly pear skin extract and Cordyceps sinensis extract, Example 1 prepared by mixing 3 parts by weight of prickly pear skin extract and Cordyceps extract each 1 part by weight Acetyl-Coei Carboxylase according to Example 4 prepared by mixing 5 parts by weight of the thorny ogapi extract and Cordyceps sinensis extract, respectively, and 6 parts by weight of the thorny Ogapi extract and Cordyceps sinensis extract per 1 part by weight of the phalanx extract Figures show activity in% of the control group.

9 is α-glucosidase activity according to Example 3 prepared by mixing 3 parts by weight of each of the thorn stem extract and Cordyceps sinensis extract per 100 parts by weight of 10000ppm phalanx extract, 100ppm prickly pear extract, 5000ppm Cordyceps sinensis extract Is a diagram showing the degree of inhibition.

Claims (2)

An antidiabetic composition comprising a mixture of 10,000 parts by weight of the phalanges extract of 10,000 ppm, 3 parts by weight of 100 ppm thorn stem extract and 3 parts by weight of 5,000 ppm Cordyceps sinensis extract as an active ingredient. The method of claim 1, Phalanx Extract or Prickly Pear Extract or Cordyceps Sinensis Extract, 8.7-12.7 parts by weight of distilled water is added to 1 part of any dry sample selected from phalanges, prickly pears, and Cordyceps sinensis, followed by shaking incubation at 50-70 ° C. for 20-28 hours, and then 50-70 at 5000-7000 rpm. The antidiabetic composition, which is prepared by mixing the obtained powder with distilled water after the filtrate obtained by centrifugation for 10 minutes and filtered to 0.35 ~ 0.55㎛ lyophilized to prepare a powder.

Images