KR101079916B1 - The funtional foods comprising the extracts from Myristica fragrans Houttuyn for the improvements and prevention of obesity - Google Patents

Abstract

The present invention relates to a nutmeg extract-containing functional food for the improvement and prevention of obesity symptoms.

Nutmeg extract according to the present invention can improve and prevent the obesity symptoms caused by the intake of high fat diet, can be useful as a functional food for the improvement and prevention of obesity symptoms to a wide range of adolescents, adults and elderly people have.

Nutmeg, obesity

Description

The funtional foods comprising the extracts from Myristica fragrans Houttuyn for the improvements and prevention of obesity

The present invention relates to a nutmeg extract-containing functional food for the improvement and prevention of obesity symptoms.

Obesity is caused by the accumulation of excess fat and high calories as fat in the body and abnormally increased body fat, which is already a big problem in the western meat-based diets, Due to changes in diet, Korea has emerged as a serious problem (Mitchell M, Armstrong DT, Robker RL, Norman RJ. Adipokines: implications for female fertility and obesity.Reproduction. 2005; 130: 583-97).

Simple obesity is manifested due to overeating, lack of exercise and decreased metabolism, and if this condition persists, obesity itself is associated with diabetes, hyperlipidemia and atherosclerosis (Kunitomi M, Wada J, Takahashi K, Tsuchiyama Y, Mimura Y, Hida K, Miyatake). N, Fujii M, Kira S, Shikata K, Maknio H. Relationship between reduced serμm IGF-I levels and accμmulation of visceral fat in Japanese men.Int J Obes Relat Metab Disord. 2002; 26: 361-9), hypertension, cardiovascular It is known to cause diseases and osteoarthritis and causes problems such as providing various causes of the disease or deactivation (Hida K, Wada J, Eguchi J, Zhang H, Baba M, Seida A, Hashimoto I, Okada T, Yasuhara A, Nakatsuka A, Shikata K, Hourai S, Futami J, Watanabe E, Matsuki Y, Hiramatsu R, Akagi S, Makino H, Kanwar YS.Visceral adipose tissue-derived serine protease inhibitor: a unique insulin-sensitizing adipocytokine in obesity Proc Natl Acad Sci US A. 2005; 102: 10610-5).

Therefore, it is important for modern people to prevent and effectively deal with obesity, which is closely related to the quality of life. As a coping method, there is a method of exercising steadily, but only a few people, and there are problems of drug rejection and drug abuse. Therefore, it is required to find a functional material having an obesity inhibitory effect and to develop a functional food that can be easily accessed by consumers.

To prevent such obesity and to lose weight, you need to reduce calorie intake and increase calorie consumption through exercise so that subcutaneous fat does not accumulate in the body. In this regard, a lot of research on dietary fiber that removes fasting while reducing calories has been recently conducted. Korean Patent Publication No. 466580 reports that dietary fiber is an indigestible component of a polymer, promotes the movement of the digestive tract in the body, and is effective in atherosclerosis, obesity, and weight loss.

In addition, many literatures are known to be effective in preventing and treating obesity. For example, Korean Patent Publication No. 842193 provides for the prevention or improvement of multiple risk factor syndromes such as visceral fat type obesity, diabetes, hyperlipidemia and hypertension selected from the group consisting of licorice hydrophobic extract, turmeric extract, clove extract and cinnamon extract. The composition for this is known, and in Korean Patent Publication No. 767051, obovatol and obovatal isolated from Magnolia obovata Thunberg (Magnoliaceae) play an important role in the growth of obesity and mast cells. It is disclosed that it can be useful for the treatment of obesity by effectively inhibiting the expression and activity of matrix degrading metalloproteinases (MMPs), and Korean Patent Publication No. 2002-0057712 discloses capsaicin, which is a pungent ingredient of red pepper, is energy. Increasing consumption is known to have an anti-obesity effect It is disclosed.

, Myristica fragrans Houttuyn)의 씨이다. 성분으로는 d-캄펜 등을 함유하고, 효능으로는 위보호 효과가 있는 것으로 공지되어 있다. 그러나 지금까지 육두구의 비만 증상의 개 선 및 예방효과는 보고된 바 없다.On the other hand, nutmeg is nutmeg (Myristicaceae)

, Myristica fragrans) is the seed of Houttuyn). It is known to contain d-camphor etc. as a component, and to have a gastric protective effect as an efficacy. However, there have been no reports of improvement and prevention of obesity symptoms.

Korean Patent Publication No. 858196 has a composition containing nutmeg extract has excellent antibacterial effect against propionibacterium acnes and can be used for acne prevention and treatment. It is disclosed that it can be widely used to reduce skin trouble and improve stability of hair products and human cleansing products, and Korean Patent Publication No. 832746 discloses a composition for controlling plant diseases including nutmeg extract, Korean Patent Publication No. 844376 The issue describes compositions for the prevention or treatment of osteoporosis.

The present invention has confirmed that it is a herbal medicine material that can improve obesity symptoms that have not been conventionally found in nutmeg extract, and completed the present invention which can be usefully used as a functional food.

An object of the present invention is to provide a functional food for the improvement and prevention of obesity symptoms produced by using a material having the function of improving and preventing obesity symptoms in natural products as a main ingredient, or added to a favorite food.

In order to achieve the above object, the present invention provides a nutmeg extract-containing functional food for the improvement and prevention of obesity symptoms.

The nutmeg extract is dried and ground in a nutmeg, and then cooled in a 10-fold extraction solvent for 1 day, and then the extract is obtained through filtration, concentration, and lyophilization, and the extraction solvent is selected. It does not specifically limit in. Extraction solvents include lower alcohols such as purified water, ethanol, methanol, isopropyl alcohol, n-butanol, polyhydric alcohols such as glycerol, propylene glycol, 1,3-butylene glycol, ethyl acetate, methyl acetate, benzene, n- Hydrocarbon solvents such as hexane, diethyl ether, dichloromethane, and the like, and the like, and one or two or more of these solvents may be mixed and used for extraction, and the extract may be flow extract, soft extract, freeze-dried product and spray It can extract using a dry method.

The present invention includes herbal extracts containing nutmeg extract as well as nutmeg extract. For example, the CAS Bom Soo Sook ^® marketed by the present applicant is a mixed herbal extract of nutmeg, cloves, health, cinnamon, sun medicinal herbs, dermis, creation, grommets, calendula and red pepper. ^® as a composition is referred to as'), cutlet bow master queue ^® composition contains a nutmeg extract is within the scope of nutmeg extract-containing compositions of the present invention.

As described in the following experimental example, in the present invention, weight change, periphery fat weight change experiment for improvement and prevention activity of obesity symptom by the Kas bow Myung Sukyu ^® composition, which is a herbal extract containing nutmeg extract as well as nutmeg extract It was confirmed through. In addition, the increase in fat tissue during obesity is histopathologically mainly caused by the hypertrophy of fat cells in the present invention, in addition to the nutmeg extract through the change in the average diameter of the ovarian periphery fat cells and abdominal fat tissue thickness experiments In addition, it was confirmed that the improvement and prevention activity of obesity symptoms by the gas life Sucu ^{® ®} composition.

Nutmeg extract according to the present invention can be prepared and used directly in the form of a pill, powder, granules, tablets, capsules, liquids, and the like, the herbal medicines known to improve the effect of obesity among herbal medicines other than the nutmeg are those skilled in the art It can be selected carefully and blended.

In addition, the present invention can be added to a variety of foods, such as chewing gum, ramen noodles, such as chewing gum, candy, confectionery, cereals, breads and other solid foods can be added to produce a functional food having an improvement and prevention effect of obesity symptoms, When prepared in a liquid formulation, it may be prepared as a pouch, beverage or tea, and may be added as a vitamin complex, health supplements, etc. to produce a functional food.

Nutmeg extract of the present invention as a material of a new functional food for the improvement and prevention of obesity symptoms can be usefully used as a functional food for the improvement and prevention of obesity symptoms to a wide range of adolescents, adults and the elderly, Hypertension, hyperlipidemia, diabetes, etc.) can be developed as a functional food for the prevention and improvement.

Hereinafter, the present invention will be described in more detail. However, these Examples are only illustrative of the present invention, and the scope of the present invention is not limited to these.

<Example 1> Preparation of herbal extracts according to the present invention (1)

1 g of 95% ethanol was added to 100 g of nutmeg, and the leachate was distilled out for 1 day, and then concentrated under reduced pressure to obtain 200 mg of nutmeg extract.

<Example 2> Preparation of herbal extracts according to the present invention (2)

3L of 70% alcohol is added to the herbal mixture (Casvum Soomyung Suq ^® composition), which contains 12 g of clove, 12 g of health, 30 g of cinnamon, 6 g of nutmeg, 100 g of dermis, 250 g of dermis, 3 g of dried fruit, 50 g of haricot, 180 g of calendula, and 6 g of red pepper. After dipping for 1 day, the leachate was distilled out and concentrated under reduced pressure to prepare a concentrate of 649 mg.

<Reference Experimental Example 1> Obesity-induced confirmation test by high fat diet

1) weight change due to high fat diet

To determine the weight change caused by the intake of high fat diet, ICR mice were selected and fed a high fat diet (40% beef tallow AIN-76A rodent diet, Dyets Inc., PA, USA) for 84 days (1) ) And high-fat diet 45% kcal high-fat pellet feed (D12451; Diet Research, PA, USA) was sacrificed in the high-fat diet group (2) fed for 84 days to observe the weight change and the results are shown in Table 1-1 and Table 1 -2 is shown.

Table 1-1

TABLE 1-2

In general, excess fat is absorbed by the supply of a high-fat diet, which significantly increases body weight. As can be seen in Table 1-1 and Table 1-2, it can be seen that in the high fat diet group (1) and the high fat diet group (2), the body weight increased significantly compared to the normal group.

2) Changes in Weight of Peripheral Ovaries by High Fat Diet

In order to compare the accumulation of adipose tissue by high-fat dietary intake, the fat pads around the ovaries and the ovaries of the high fat diet group (1) and the high fat diet group (1) and the ovary were removed together with the normal group and the ovaries were removed. After the absolute weight of the fat pad was measured and shown in Table 2-1 and Table 2-2.

TABLE 2-1

Table 2-2

As can be seen from Table 2-1 and Table 2-2, in the high fat diet group (1) and the high fat diet group (2), the weight of the ovary fat was significantly increased compared to the normal group, resulting in high fat diet. It can be confirmed that obesity is induced by this accumulation.

3) Histopathological Changes of Peripheral and Abdominal Fats due to High Fat Diet

The tissues of the ovarian and abdominal fat of the high fat diet group (1) and the high fat diet group (2) together with the normal group to confirm the histopathological changes of the ovarian fat and abdominal fat caused by the intake of a high fat diet Pathological changes were observed.

After cutting small circumferential fat and abdominal fat fixed in 10% neutral formalin and embedding with paraffin, embedding tissue was cut into 3 ~ 4 ㎛ thickness using microtome, and hematoxylin & Stained with eosin. The average diameters of peri-ovarian fat cells and abdominal fat cells of the high fat diet group (1) and the high fat diet group (2) together with the normal group were measured by measuring the diameters of the ten adipocytes. It is shown to 3-2. Abdominal fat thickness was measured using an automated image analysis process.

Table 3-1

Table 3-2

As can be seen from Table 3-1 and Table 3-2, in the high fat diet group (1) and the high fat diet group (2), significant fat cell hypertrophy was observed in the ovarian and abdominal fat tissues, respectively, compared to the normal group. The thickness of abdominal adipose tissue was significantly increased.

Experimental Example 1 Obesity Symptoms Improvement Effect by Inhibiting Weight Gain of Extracts of the Present Invention

1) In order to confirm the improvement effect of obesity symptoms by the administration of nutmeg extract, the weight change was evaluated using the obesity model of ICR mice. Experimental method is a high-fat diet group (1) is selected from the ICR mouse high-fat feed (40% beef tallow AIN-76A rodent diet, Dyets Inc., PA, USA) and extract obtained in Example 1 of the nutmeg Diluted to 20mg / ml (5% ethanol) and administered orally once a day at a concentration of 10ml / kg for 84 days.The control group was a high fat diet (40% beef tallow AIN-76A rodent diet, Dyets Inc., PA, USA) alone for 84 days.

Comparing the weight change at the expense of the high fat diet group (1) and the control group the results are shown in Table 4-1.

Table 4-1

2) In addition, as another experimental method, the high fat diet group (2) was selected as a high fat diet 45% kcal high fat pellet feed (D12451; Diet research, PA, USA) and Example 2 (Casvum life succu ^® composition) The extract obtained in the above was diluted to 173.07mg / 20ml as the total crude drug dose and orally administered once a day at 20ml / kg for 84 days. It was administered for 84 days.

Comparison of the weight change of the high fat diet group (2) and the control group is shown in Table 4-2.

Table 4-2

As can be seen from Table 4-1 and Table 4-2, Examples 1 and 2 according to the present invention are 30.55% and 60.39, respectively, in weight gain compared to the high fat diet group (1) and the high fat diet group (2), respectively. It can be seen that the obesity inhibitory effect is excellent even though high fat diet was administered together by% inhibition.

Experimental Example 2 Obesity Symptoms Improvement by Inhibition of Increased Peripheral Fat Weight of Extracts of the Present Invention

1) In order to confirm the effect of the present invention on the improvement of obesity symptoms by administering in the same manner as 1) of Experimental Example 1 and then sacrifice the experimental animals in the same way as 2) of Reference Experimental Example 1 to change the fat weight around the ovary The measurement results are shown in Table 5-1.

Table 5-1

2) As another method, in order to confirm the effect of improving the obesity symptoms of the extract of the present invention administered in the same manner as in Experimental Example 1 2) and then sacrificed the experimental animals in the same manner as in Reference Experimental Example 1 2) Changes in the weight of ovarian periphery of 2 were measured and the results are shown in Table 5-2.

Table 5-2

As can be seen from Table 5-1 and Table 5-2, Examples 1 and 2 according to the present invention, respectively, compared to the high fat diet group (1) and the high fat diet group (2), respectively, to increase the weight of the ovarian periphery fat, respectively. Inhibition of 31.59, 76.53%, even if high-fat diet was administered together, it can be seen that the effect of obesity is excellent.

Experimental Example 3 Obesity Symptoms Improvement by Histopathological Changes of Peripheral and Abdominal Fats of the Extracts of the Present Invention

1) In order to confirm the effect of the present invention on the improvement of obesity symptoms, the same method as 1) of Experimental Example 1 and then administered to sacrifice the animals in the same way as 3) of Reference Experimental Example 1 of the ovary and abdominal fat Histopathological changes are shown in Table 6-1.

Table 6-1

As can be seen in Table 6-1, Example 1 according to the present invention suppressed the fat cell size of ovarian adipose tissue and abdominal adipose tissue 13.96%, 2.86%, compared to the high fat diet group (1), abdominal fat The 23.66% thickness was suppressed by the obesity suppression effect even when high fat diet was administered together.

2) In addition, the histopathological changes of the ovarian periphery and abdominal fat were measured in the same manner as in Reference Experiment 1 3) by administering the same method as Experimental Example 2) and then sacrificing the experimental animals. -2 is shown.

Table 6-2

As can be seen from Table 6-2, Example 2 according to the present invention suppressed the fat cell size of ovarian adipose tissue and abdominal adipose tissue by 55.30%, 48.45%, compared to the high fat diet group (1), abdominal fat It can be seen that the obesity inhibitory effect is excellent even though high fat diet was administered together by suppressing the thickness of 63.81%.

Hereinafter, various functional foods may be prepared using the herbal extract according to the present invention as a main ingredient.

Preparation Example 1 Preparation of Granules

Granules were prepared in the usual manner with the following component content per packet.

Dry powder of nutmeg extract prepared in Example 1 ....... 200 mg

Lactose ......................................... 100 mg

Talc ... mg

Preparation Example 2 Preparation of Tablet

Tablets were prepared in the usual manner with the following component content per tablet.

Dry powder of nutmeg extract prepared in Example 1 ........ 300 mg

Corn Starch ......................................... 100 mg

Lactose ......................................... 100 mg

Magnesium Stearate ......................................... 2 mg

Production Example 3 Preparation of Capsule

Capsules were prepared in the usual manner with the following component content per capsule.

Concentrate of nutmeg extract prepared in Example 2. 300 mg

Corn Starch ......................................... 100 mg

Lactose ......................................... 100 mg

Magnesium Stearate ......................................... 2 mg

Production Example 4 Preparation of Liquid

The liquid formulation was prepared in the usual manner with the following component content per 100 ml of the liquid formulation.

Concentrate of nutmeg-containing extract prepared in Example 2 ...... 500 mg

Isomerized sugar ......................................... 10 g

Mannitol ... 5 g

Purified water ...............................

Preparation Example 5 Preparation of Candy

Candy alcohol was prepared by combining 45 wt% of sugar alcohol, 49.8 wt% of reduced syrup, and 0.2 wt% of fragrance, and 5 wt% of the nutmeg extract prepared in Example 1 above.

Preparation Example 6 Preparation of Biscuits

Force 1st class 21.59 wt%, gravity first class 22.22 wt%, white sugar 4.80 wt%, salt 0.73 wt%, glucose 0.78 wt%, palm shortening 11.15 wt%, ammo 1.54 wt%, sodium bicarbonate 0.17 wt%, sodium bisulfite 0.16 wt% , Rice flour 1.45%, Vitamin B1 0.0001%, Vitamin B2 0.0001%, Milk flavor 0.04%, Water 21.3298%, Whole milk powder 1.16%, Substitute powder 0.29%, calcium phosphate 0.03%, Spray salt Biscuits were prepared in a conventional manner by combining 0.29 wt%, 7.27 wt% of spray oil, and 5 wt% of the nutmeg extract prepared in Example 1.

Claims (6)

delete Nutmeg, cloves, health, cinnamon, sun medicine, dermis (창출,), creation, bakbak, Hyunho color, red pepper, respectively, 1: 1: weight ratio of herbal Functional food for improving and preventing obesity symptoms, characterized by containing 70% alcohol extract. delete delete delete delete