KR101075474B1 - Selective lighting system for fluorescence image acquisition - Google Patents

Abstract

The present invention relates to a selective light source system for acquiring a fluorescence image, and more particularly, a plurality of rotating light source modules selectively irradiate a fluorescence source according to a wavelength of fluorescence generated from a fluorescence material to generate a fluorescence image generated from a subject. A selective light source system for fluorescence image acquisition used for acquisition.

The selective light source system for acquiring the fluorescence image of the present invention can easily obtain a fluorescent light source by irradiating the fluorescent light source even when the wavelength range of the specific wavelength band and the filter is different, and reduces the cost for the light source, Can improve the wavelength selection range.

Molecular Optics, Small Animal Image, Fluorescent Light Source, Fluorescent Image, LED

Description

SELECTIVE LIGHTING SYSTEM FOR FLUORESCENCE IMAGE ACQUISITION}

The present invention relates to a selective light source system for acquiring a fluorescence image, and more particularly, a plurality of rotating light source modules selectively irradiate a fluorescence source according to a wavelength of fluorescence generated from a fluorescence material to generate a fluorescence image generated from a subject. A selective light source system for fluorescence image acquisition used for acquisition.

Molecular optical imaging is a technique for imaging molecular information by outputting photons as image signals. Such molecular images can be classified into nuclear medical imaging using photons generated by the reaction between crystals of a detector and radiation, and optical images using visible and near-infrared light generated in a living body as image signals.

In addition, the optical image may be classified into a bioluminescence image and a fluorescence image. Here, the bioluminescence image uses a principle that the light emitting material emits light, and the fluorescence image is a cell, tissue, Alternatively, the fluorescent material absorbs light emitted from the outside and excites and emits light having a longer wavelength than the irradiated light source.

At this time, the fluorescent protein gene required for the fluorescence image acquisition has been developed such as GFP (Green Fluorescene Protein), RFP (Red Fluorescene Protein), BFP (Blue Fluorescene Protein) and YEP (Yellow Fluorescene Protein), in particular, GFP or GFP EGFP (Enhanced GFP), a variant of, is widely used, and increasingly diverse and brighter fluorescent protein genes are being developed.

Since the fluorescence image is mainly obtained by using a Charged Coupled Device (CCD) camera, the fluorescence image can be obtained quickly and has the advantage of not sacrificing animals such as mice.

However, since the fluorescent image uses a light source of a specific wavelength to obtain an image and at the same time uses a filter of a specific wavelength band, a lamp or a lamp used as a light source of a fluorescent imaging apparatus which is most commonly used at present. Lasers have difficulty in imaging due to a weak amount of light in a specific wavelength range, and require users to directly change filters according to a required wavelength, and have a high price point and limited selection of wavelengths.

In addition, the fluorescence image has a disadvantage in that the fluorescence image cannot be obtained when the wavelength range of the filter is different in observing the fluorescence dye as well as in the fluorescence protein gene administered to the animal.

In addition, when GFP is used as the fluorescent protein gene, since each GFP emits its own wavelength among the GFP, a filter suitable for the excitation wavelength and emission wavelength of the gene must be selected for accurate observation. Difficulties in selecting a filter according to.

Moreover, in the United States, a leading country in the field of medical imaging devices, we have developed a device that can test bloodless blood glucose using only optical systems and are currently undergoing FDA approval procedures. While the system is being developed to monitor 24 hours a day, domestic imaging equipment is in very early stages, and due to the technical limitations on the light source, the technical limitations on the system, and the high cost of the light source, I can't.

Therefore, in obtaining a fluorescent image of the optical image as described above, there is an urgent need for a fluorescent image acquisition system capable of obtaining a fluorescent image by applying a light source having a low cost, a long lifetime, and a large wavelength selection range.

The present inventors have made efforts to solve the above problems, and as a result, it is possible to easily obtain a fluorescence image in a specific wavelength region, to reduce the cost, and to limit the wavelength selection range and the difficulty of filter selection according to the inherent wavelength range. In addition, the present invention has been completed by developing a selective light source system for obtaining a fluorescence image that can easily obtain a fluorescence image even when the wavelength range of the filter is different.

Accordingly, an object of the present invention is to provide a selective light source system for obtaining a fluorescence image that can easily acquire a fluorescence image in a specific wavelength band region.

In addition, another object of the present invention is to provide a selective light source system for fluorescence image acquisition that can reduce the cost.

In addition, another object of the present invention is to provide a selective light source system for fluorescence image acquisition that can overcome the limitation of the wavelength selection width and the difficulty of filter selection according to the intrinsic wavelength band.

In addition, another object of the present invention is to provide a selective light source system for obtaining a fluorescence image that can be easily obtained even when the wavelength range of the filter is different.

The present invention for achieving the above object is a selective light source system for obtaining a fluorescent image to obtain a fluorescent image by selectively irradiating a fluorescent light source to the subject, the selective light source system for obtaining the fluorescent image, the axis of rotation and the axis of rotation An upper module made of a step motor for rotating the motor; A center module disposed below the upper module, the center module including a plurality of light source modules and the plurality of light source modules, the magazine being connected to the rotating shaft to be rotated by the rotating shaft; And a lower module disposed below the central module and configured to include a light transmitting window that irradiates a light source irradiated from the light source module with a test object. 2.

In a preferred embodiment, the upper module further includes a gear wheel connecting between the step motor and the rotary shaft.

In a preferred embodiment, the light source module is a light emitting diode (LED) light source.

In a preferred embodiment, the light source module is characterized in that the circular spaced apart from the magazine.

In a preferred embodiment, the light source module is characterized in that 6 to 12 are installed in the magazine.

In a preferred embodiment, the magazine is characterized in that the rotational movement in accordance with the rotation of the rotary shaft.

In a preferred embodiment, the selective light source system for acquiring the fluorescence image further comprises an electrode rod housing for supplying electricity to the light source module.

In a preferred embodiment, the selective light source system for obtaining the fluorescence image further comprises a control touch screen for controlling the stop motor.

The present invention has the following excellent effects.

First, the selective light source system for acquiring the fluorescence image of the present invention can adjust the amount of light even in a specific wavelength band, and thus can easily obtain the fluorescence image accordingly.

In addition, the selective light source system for acquiring the fluorescence image of the present invention can reduce the cost compared to the prior art when acquiring the fluorescence image.

In addition, the selective light source system for acquiring the fluorescence image of the present invention can improve the wavelength selection width of the light source, thereby overcoming the limitation of the wavelength selection width and the difficulty of filter selection according to the inherent wavelength band. You can prevent the hassle.

In addition, the present invention can easily obtain a fluorescent image even when the wavelength range of the filter is different.

The terms used in the present invention were selected as general terms as widely used as possible, but in some cases, the terms arbitrarily selected by the applicant are included. In this case, the meanings described or used in the detailed description of the present invention are considered, rather than simply the names of the terms. The meaning should be grasped.

Hereinafter, with reference to the accompanying drawings and preferred embodiments will be described in detail the technical configuration of the present invention.

However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Like reference numerals designate like elements throughout the specification.

1 is a cross-sectional view illustrating a selective light source system for obtaining a fluorescent image according to an embodiment of the present invention, Figure 2 is a view illustrating a selective light source system for obtaining a fluorescent image according to an embodiment of the present invention 3 is a perspective view illustrating a selective light source system for obtaining a fluorescence image according to an exemplary embodiment of the present invention.

4 to 5 are perspective views illustrating a selective light source system for obtaining a fluorescence image according to an embodiment of the present invention, and FIG. 6 is a selective light source for obtaining a fluorescence image according to an embodiment of the present invention. This is a schematic diagram for explaining a system, which will be described below.

First, as shown in FIGS. 1 to 3, the selective light source system 100 for acquiring a fluorescence image according to an embodiment of the present invention acquires a fluorescence image by selectively irradiating a subject (not shown) with a fluorescent light source. An optional light source system 100 for acquiring a fluorescence image, which includes a rotating shaft 143 and a step motor 140 for rotating the rotating shaft 143, and is driven by a control signal to drive the step motor 140. When the rotating shaft 143 is rotated, the magazine 121 connected to the rotating shaft 143 and the plurality of light source modules 120 are disposed is rotated so that the fluorescent light source is irradiated to the subject through the light source transmission window 111. do.

In addition, in the selective light source system 100 for acquiring a fluorescence image according to an embodiment of the present invention, when a user inputs a desired light source and a wavelength band from the control touch screen 150, the control signal is controlled by the step motor 140. Is transmitted, and the step motor 140 receives the received light from the light source module 120 selected by the rotation of the rotation shaft 143 and the magazine 121 to irradiate a fluorescent light source with respect to a subject to obtain a fluorescence image. Driven.

In detail, the selective light source system for acquiring the fluorescence image according to the embodiment of the present invention includes the configuration of the upper module (a), the center module (b) and the lower module (c).

The upper module a includes a rotation shaft 143 and a step motor 140.

The rotating shaft 143 is provided for selective rotation of the fluorescent light source for irradiating a light source to the subject.

The step motor 140 is installed at one side of the upper module a to rotate the rotating shaft 143 provided for the selective rotation of the fluorescent light source.

In addition, the step motor 140 rotates the magazine 121 in which the fluorescent light source is disposed at a predetermined angle every time a control signal is received for selective rotation of the fluorescent light source.

Therefore, the user can arrange the module light source 120 to be desired for the expression of the fluorescent protein gene of the subject through the step motor 140.

Here, the upper module a including the rotary shaft 143 and the step motor 140 has a step motor gear 141a and a rotary shaft cog wheel to rotate the rotary shaft 143 driven by the driving of the step motor 140. 141b is further included.

At this time, by such a step motor gear 141a and the rotary shaft gear 141b, it is possible to implement an automated system that is automatically operated when the control signal is received. Therefore, it is possible to prevent the invasion of bacteria in the hassle and sterility experiments that need to replace the filter according to the conventional wavelength.

The central module (b) is disposed below the upper module (a), and includes a plurality of light source modules 120, magazines 121, first connection holders 110b, second connection holders 110a, and magazines. A sheet 122.

The plurality of light source modules 120 irradiate the fluorescent light source directly to the subject disposed under the selective light source system 100 for obtaining the fluorescence image according to an embodiment of the present invention, and is injected into the tissue of the subject. It stimulates protein genes to shine.

In addition, the plurality of light source modules 120 includes, for example, a large selection range of wavelengths and a light emitting diode (LED) light source module having a longer lifetime than a conventional lamp and laser light source.

In this case, each of the plurality of light source modules 120 including the LED light source module includes an insulation washer 161, an LED light source 162, a housing 163, and an electrode substrate 164, as shown in FIG. 4. .

The magazine 121 arranges the plurality of light source modules 120, and the plurality of light source modules 120 disposed in the magazine 121 are spaced apart in a circle in the magazine 121. Preferably, the light source module 120 may be installed in a circle of 6 to 12 in accordance with the frame of the magazine 121.

The first connection support fixture 110b and the second connection support fixture 110a are interposed between the center module (b) and the upper module (a) and the center module (b) and the lower module (c), respectively. (a) and the central module (b).

In this case, the first connection support fixture 110b and the second connection support fixture 110a are installed to be detachably attached to facilitate the detachment of the light source module 120.

The magazine sheet 122 serves as a ring connecting the rotating shaft 143, the first connection support 110b and the magazine 121 of the upper module (a), the magazine by the rotation of the rotating shaft 143 The role of rotating the 121 is performed.

The lower module (c) is disposed below the center module (b), and includes a rotation shaft holder 142 and a light source transmission window 111.

The rotating shaft holder 142 is installed on the second connecting support holder 110a, and serves to fix the magazine 121 so that the rotating shaft 143 for rotating the magazine 121 is not separated during operation.

The light source transmission window 111 is installed at one side of the second connection holder 110a of the lower module c, and when the user generates a fluorescent light source in the desired light source module 120, the fluorescent light source is irradiated to the subject. The role is played.

That is, since the fluorescent light source is irradiated to the subject through the light source transmission window 111, the fluorescent light source light is prevented from being dispersed and reduced through the light source transmission window 111 so that the subject can be irradiated with precision. In addition, accurate and clear fluorescence images of the subject can be obtained.

In addition, the selective light source system 100 for obtaining a fluorescence image according to an embodiment of the present invention further includes an electrode rod housing 130.

As shown in FIG. 5, the electrode rod housing 130 includes a contact housing 165, a first insulation portion 166, an electrode rod 167, and a second insulation portion 168.

In this case, the electrode rod housing 130 is installed over the upper module (a) and the center module (b) to supply electricity to the light source module 120, preferably, the light source module 120 and the light source transmission Installed in a straight line with the window 111 is connected to the electrode substrate 164 of the light source module 120 to supply electricity to the light source module 120.

Therefore, the electrode rod housing 130 installed in line with the light source module 120 and the light source transmission window 111 in this way does not require an additional electrical connection for supplying electricity to the light source module 120. In addition, electricity may be easily supplied only to the selected light source module 120, and accordingly, detachment of the magazine 121 and the light source module 120 may be easily performed.

In addition, the selective light source system 100 for obtaining a fluorescence image according to an exemplary embodiment of the present invention further includes a control touch screen 150.

As shown in FIG. 6, the control touch screen 150 drives the stop motor 140 by inputting and manipulating the wavelength band input of the fluorescent light source by a simple touch type, and currently emitting light by the information display of the fluorescent light source. Fluorescent light source, the wavelength of the fluorescent light source and the brightness of the fluorescent light source can be confirmed.

As described above, the present invention can control the amount of light even in a specific wavelength range according to the application of the LED light source module as a light source module for acquiring a fluorescent image as described above, it is possible to obtain an easy fluorescent image accordingly The cost can be reduced compared to the conventional method.

In addition, since the present invention can improve the wavelength selection width of the light source, it is possible to overcome the limitation of the wavelength selection width and the difficulty of selecting the filter according to the inherent wavelength band, thereby preventing user's hassle.

In addition, the present invention can easily obtain a fluorescent image even when the wavelength range of the filter is different.

As described above, the present invention has been illustrated and described with reference to preferred embodiments, but is not limited to the above-described embodiments, and is provided to those skilled in the art without departing from the spirit of the present invention. Various changes and modifications will be possible.

1 is a cross-sectional view illustrating a selective light source system for fluorescence image acquisition according to an embodiment of the present invention.

2 is a perspective view illustrating a selective light source system for obtaining a fluorescence image according to an exemplary embodiment of the present invention.

3 is a cross-sectional view illustrating a selective light source system for obtaining a fluorescence image according to an exemplary embodiment of the present invention.

4 to 5 are perspective views illustrating a selective light source system for obtaining a fluorescence image according to an embodiment of the present invention.

FIG. 6 is a schematic diagram illustrating a selective light source system for obtaining a fluorescence image according to an exemplary embodiment of the present invention. FIG.

<Description of the code | symbol about the principal part of drawing>

100: selective light source system for fluorescence image acquisition

a: upper module b: center module

c: lower module 111: light transmitting window

120: light source module 121: magazine

130: electrode rod housing 140: step motor

143: axis of rotation

Claims (8)

A selective light source system for acquiring a fluorescence image by selectively irradiating a subject with a fluorescent light source, wherein the selective light source system for acquiring the fluorescence image, An upper module comprising a rotating shaft and a step motor for rotating the rotating shaft; A central module fixed to the lower side of the upper axis of the upper module, the center module including a plurality of light source modules and a magazine in which the plurality of light source modules are disposed and rotated by the rotating axis; And A lower module disposed below the center module, the lower module including a light source transmitting window that irradiates a light source irradiated from the light source module with a subject; Selective light source system for obtaining a fluorescence image comprising a. The method of claim 1, The upper module further comprises a gear wheel for connecting between the step motor and the rotating shaft. The method of claim 1, The light source module is a selective light source system for obtaining a fluorescent image, characterized in that the LED (Light Emitting Diode) light source. The method of claim 1, The light source module is a selective light source system for obtaining a fluorescence image, characterized in that spaced apart from the magazine. The method of claim 1, The light source module is a selective light source system for obtaining a fluorescence image, characterized in that 6 to 12 are installed in the magazine. The method of claim 1, The magazine is a selective light source system for obtaining a fluorescence image, characterized in that the rotational movement in accordance with the rotation of the rotation axis. The method of claim 1, The selective light source system for acquiring the fluorescence image further comprises an electrode rod housing for supplying electricity to the light source module. The method of claim 1, The selective light source system for acquiring the fluorescence image further comprises a control touch screen for controlling the step motor.

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