KR101068533B1 - Indole-3-acetic acid for preventing hair loss and enhancing hair restoration, nanocomposite comprising the compound, and composition comprising the compound or the nanocomposite - Google Patents

Abstract

The present invention relates to indole-3-acetic acid as a compound for preventing hair loss and hair growth, and to a nanohybrid complex of the compound, a composition for preventing hair loss and hair growth, including the same, and more particularly, having an excellent hair loss prevention and hair growth promoting effect. Indole-3-acetic acid; Nano-hybrid composite for preventing hair loss and promoting hair growth, characterized in that indole-3-acetic acid is inserted between layers of the layered metal salt, and has excellent stability against external environmental changes, and excellent drug delivery ability; And it relates to a composition for promoting hair growth comprising the compound or the nanohybrid complex.

Description

[Indole-3-acetic acid for preventing hair loss and enhancing hair restoration, nanocomposite comprising the compound, and composition comprising the compound or the nanocomposite}

The present invention relates to indole-3-acetic acid as a compound for preventing hair loss and hair growth, and to a nanohybrid complex of the compound, a composition for preventing hair loss and hair growth, including the same, and more particularly, having an excellent hair loss prevention and hair growth promoting effect. Indole-3-acetic acid; Nano-hybrid composite for preventing hair loss and promoting hair growth, characterized in that indole-3-acetic acid is inserted between layers of the layered metal salt, and has excellent stability against external environmental changes, and excellent drug delivery ability; And it relates to a composition for promoting hair growth comprising the compound or nanohybrid complex.

Hair loss refers to a condition in which hair is loose and lost or lost, and human hair has about 100,000 to 150,000 hairs, and has hair growth and dropping with its own cycles of anagen, catagen, and telogen. Repeat, and on average, about 100 hairs fall naturally every day. The mechanism of hair loss has not been precisely identified, but studies to date indicate that excessive sebum production, malnutrition of the hair roots, allergies, bacterial infections, Genetic factors, mental stress, environmental factors such as air pollution or food, and aging are presumed to be the causes. Alopecia occurred only in the middle and middle age group in the past, but recently occurs frequently among young people in their 20s and 30s.

The most common type of male hair loss is male pattern baldness or alopecia, which gradually develops over the years, most pronounced in the crown of the head and progresses to the frontal region. Female hair loss occurs as the hair becomes thinner and more dispersed, often after menopause.

Many studies are being conducted to alleviate or treat alopecia, and in the cosmetics or pharmaceutical industry for a long time to develop new substances for stimulating hair growth or alleviating hair loss phenomenon, female hormones, blood flow Drug therapy using hair stimulants and hair transplantation have been developed. Currently, the most widely used drugs for the treatment of hair loss include 2,4-diamino-6-piperidinopyrimidine-3-oxide (also known as 'minoxidil' formulations, US patents). 4,139,619 and 4,596,812) and finasteride, which are specific inhibitors of type II 5α-reductase.

Minoxidil preparation is a drug that induces hair growth by increasing blood flow and nourishing hair roots through vasodilation effect, and is known to be effective in alleviating the symptoms of hair loss at the kiln site, and it is used as an active ingredient. Is marketed under the trade name Rogaine (trade name of Pharmacia & Upjohn Company). Rogaine is known to reduce hair loss and promote hair growth by up to 10% in men with alopecia areata, but should be applied directly to the scalp area externally, regularly and over a long period of time, and in areas other than the kiln area. There is a disadvantage in that it does not have a very good effect on hair loss.

Drugs using finasteride as an active ingredient are commercially available under the trade name Propecia (trade name of Propecia, Merck & Co., Inc.). It is known to inhibit hair loss by preventing the conversion to (Dihydrotestosterone, DHT). However, this also requires constant and regular doses, and some patients have the problem that side effects such as decreased libido, erectile dysfunction.

In addition, a method of using the composition or a hair transplant method based on natural plant extracts is used as a method for preventing hair loss and promoting hair growth. However, natural plant extracts do not have a positive effect on hair growth because the condition of hair or alopecia varies from person to person, and due to skin irritation or unpleasant odors, it is difficult to sustain long-term use. There are problems such as detachment after planting, the need for additional transplant surgery, and high cost.

Therefore, while being able to overcome the problems of minoxidil formulations or finasteride formulations, it is required to develop hair loss prevention and hair growth promoting compounds that have better effects and efficacy than other natural plant extract compositions or hair transplantation methods, and have excellent drug delivery and stability. come.

Meanwhile, indole-3-acetic acid is a growth hormone of plant tissues, and is widely distributed at the ends of leaves, roots, and stems of plants, and is represented by the following formula.

             (One)

Indole-3-acetic acid is used as a cosmetic to improve wound healing and wrinkles when applied to the skin. Recently, indole-3-acetic acid is used for acne skin, which can improve the skin aggravated by acne. It is used as a product. For example, International Publication No. WO 99/00079 discloses that when a cosmetic containing indole-3-acetic acid is applied to a wound site, it promotes the movement of skin cells and is effective in healing wounds and improving skin wrinkles. In Korean Patent No. 99-36863, a cosmetic for improving acne by using a combination of carbamide peroxide and α-hydroxy acid and indole-3-acetic acid is disclosed.

In addition, in Korean Patent No. 405410 (registered on Nov. 3, 2003), indole-3-acetic acid, which is easily browned by light and heat, is discolored due to the above mentioned beneficial effects, and improves the problem of occurrence of odor. Acetic acid microcapsule liposome preparations are disclosed. However, no studies on hair growth of indole-3-acetic acid have been reported.

Meanwhile, various drug carriers have been developed to effectively deliver the above-mentioned drugs into the human body and improve stability to the external environment. In particular, a layered inorganic compound incorporating a drug active ingredient may include various materials between layers. So much research is being done. The present inventors have also been continuously conducting research on such drug carriers, and in particular, ion-exchange, co-exchange of anion active materials between the layers of layered double hydroxides (LDH) and zinc basic salts (ZBS) It can be easily inserted through acupuncture, recombination, etc., and the size can be adjusted in nano units, and it has been disclosed that it is highly applicable in the fields of separation technology, optics, medicine, and engineering. Specifically, the present inventors reported the results of research on cosmetics containing nanohybrid materials of indole-3-acetic acid and layered metal salts in Korean Patent Registration No. 10-439299 (registered on June 28, 2004), and related studies Continuing with the present invention, nanohybrid complexes having active ingredients such as indole-3-acetic acid between layers of metal double layered hydroxides and layered metal salts such as zinc base salts can further improve hair loss prevention and hair growth promoting effects. Revealed.

Accordingly, the first problem to be solved by the present invention is to provide a new use of indole-3-acetic acid as a compound for preventing hair loss and promoting hair growth.

The second problem to be solved by the present invention is to provide a hybrid nanocomposite (Hybrid nanocomposite) for preventing hair loss and hair growth characterized in that the indole-3-acetic acid is inserted between the layers of the layered metal salt.

A third object of the present invention is to provide a composition for preventing hair loss and promoting hair growth, including a nanohybrid complex in which indole-3-acetic acid is inserted between layers of a layered metal salt.

In order to solve the first problem, the present invention provides a compound for preventing hair loss and hair growth represented by the following formula (1):

        (One)

The compound of Formula 1 is indole-3-acetic acid, the first hair loss prevention and hair growth promoting effect was demonstrated through the present invention.

The present invention provides a nanohybrid complex in which the indole-3-acetic acid of Chemical Formula 1 is inserted between layers of a layered metal salt.

According to one embodiment of the present invention, the layered metal salt may be selected from metal layered double hydroxide (LDH) or zinc basic salt (ZBS).

According to a preferred embodiment of the present invention, the LDH may be represented by the following formula:

[M ²⁺ _(1-x) N ³⁺ _x (OH) ₂ ] A _x yH ₂ O

Wherein M ²⁺ is a divalent metal cation, N ³⁺ is a trivalent cation, A is indole-3-acetic acid, x is a number ranging from 0.1 to 0.4, and y is a positive number greater than zero to be. In addition, M ²⁺ is selected from the group consisting of Mg ²⁺ , Ni ²⁺ , Cu ²⁺ and Zn ²⁺ , and N ³⁺ is Al ³⁺ , Fe ³⁺ , V ³⁺ , Ti ³⁺ and Ga ^3. It is preferably selected from the group consisting of ⁺ .

According to another preferred embodiment of the present invention, the ZBS may be represented by the following formula:

Zn ₅ (OH) ₈ A.nH ₂ O or Zn ₃ (OH) ₄ A ₂ nH ₂ O

Wherein A is indole-3-acetic acid and n is a positive integer.

The present invention provides a composition for preventing hair loss and promoting hair growth containing indole-3-acetic acid or indole-3-acetic acid containing a nanohybrid complex inserted between layers of a layered metal salt.

According to one embodiment of the present invention, the hair growth promoting composition comprises an effective amount of the indole-3-acetic acid can exhibit hair loss prevention and hair growth promoting effect. For example, the hair growth promoting composition preferably comprises 0.01 to 5% by weight of the nano-hybrid complex in which indole-3-acetic acid is intercalated between the layered metal salt, 0.01 to 1% by weight of indole-3-acetic acid It is desirable to.

According to another embodiment of the present invention, the hair growth promoting composition may be selected from the group consisting of lotion, emulsion, cream, essence or spray formulation.

According to the present invention, it was proved that indole-3-acetic acid, which was used only as a raw material for cosmetics, had an excellent effect in preventing hair loss and promoting hair growth, and in particular, a nanohybrid composite having indole-3-acetic acid intercalated between layers of a layered metal salt; The composition for preventing hair loss and promoting hair growth comprising the nanohybrid composite has excellent stability against external environmental changes such as heat, light, air, and oxygen, good dispersibility in hydrophilic and lipophilic media, and an active ingredient. Phosphorus indole-3-acetic acid can be released slowly to have a continuous effect, maximizing hair growth promoting effect.

Hereinafter, the present invention will be described in more detail.

The inventors have found that indole-3-acetic acid represented by the following formula (1) has an excellent effect on the prevention of hair wrinkles and the promotion of hair growth, as well as skin wrinkle improvement and wound healing effects known in the art:

      (One)

In addition, in the present invention, by providing a nano-hybrid complex carrying the active ingredient between the layers of the layered metal salt known as a sustained release drug delivery system, it was possible to maximize the hair loss prevention and hair growth promoting effect of indole-3-acetic acid.

In the present invention, the layered metal salt used as a carrier for the hair regrowth component may be selected from metal layered double hydroxide (LDH) or zinc basic salt (ZBS).

More specifically, the LDH may be represented by the following formula,

[M ²⁺ _(1-x) N ³⁺ _x (OH) ₂ ] A _x yH ₂ O

Wherein M ²⁺ is a divalent metal cation, N ³⁺ is a trivalent cation, A is indole-3-acetic acid, x is a number ranging from 0.1 to 0.4, and y is a positive number greater than zero to be. In addition, M ²⁺ is selected from the group consisting of Mg ²⁺ , Ni ²⁺ , Cu ²⁺ and Zn ²⁺ , and N ³⁺ is Al ³⁺ , Fe ³⁺ , V ³⁺ , Ti ³⁺ and Ga ^3. It is preferably selected from the group consisting of ⁺ .

In addition, the ZBS may be represented by the following formula,

Zn ₅ (OH) ₈ A.nH ₂ O or Zn ₃ (OH) ₄ A ₂ nH ₂ O

Wherein A is indole-3-acetic acid and n is a positive integer.

In addition, according to the present invention, a method for producing a nano-hybrid complex for promoting hair growth coprecipitates an aqueous solution of a metal salt with a basic material to form a precipitate of a layered metal salt having an anion inserted therebetween, and indole-3-anion between the layers. Ion exchange with acetic acid to produce nanohybrid complexes.

Metal salts used as starting materials in the preparation of the nanohybrid composite are, for example, ZnCl ₂ , Zn (NO ₃ ) ₃ , Zn (CH ₃ COO) ₂ , MgCl ₂ , AlCl ₃ , CaCl ₂ , Mg (NO ₃₎ ) _2, Al (NO ₃₎ can be given _three or hydrates thereof.

In addition, the solvent used for the coprecipitation may be water, alcohol or a mixture thereof, ethanol is preferred as the alcohol. The concentration of metal ions used in the coprecipitation method is, for example, in the range of 0.01 mol to 5 mol, and the amount of the drug component is used in a molar ratio of about 0.1 to 10 times based on the total moles of the metal component. In the precipitation reaction, precipitation may be induced by adding a base if necessary. Suitable bases include, for example, alkali metal hydroxides or amines. The pH of the reaction solution is 4 to 11, preferably 6 to 8, and the reaction temperature is 0 ° C to 5 ° C, preferably 0 ° C to 10 ° C. The reaction time is preferably 30 minutes or more. In addition, during the reaction, it is preferable to continuously add nitrogen or an inert gas, and to react by blocking the light.

The present invention is characterized by providing a composition for promoting hair growth that ultimately contains a nanohybrid complex in which indole-3-acetic acid or indole-3-acetic acid is intercalated between layers of a layered metal salt. At this time, the content of indole-3-acetic acid in the hair growth promoting composition is an effective amount capable of exhibiting hair loss prevention and hair growth promoting effect, for example, the hair growth promoting composition is indole-3-acetic acid in the interlayer of the layered metal salt It is preferable to include 0.01 to 5% by weight of the inserted nanohybrid complex, and preferably 0.01 to 1% by weight of indole-3-acetic acid.

On the other hand, the composition for preventing hair loss and promoting hair growth according to the present invention may further comprise a pharmaceutically acceptable carrier, in addition to the compound of formula 1 or the nanohybrid complex as an active ingredient, within a range that does not alleviate the main effect, It may further comprise a substance that promotes skin absorption and other substances that enhance the efficacy. Examples of the carrier include one or more carriers selected from the group consisting of excipients, binders, lubricants, disintegrants, lubricants, coatings, emulsifiers, suspensions, solvents, stabilizers, absorption aids, water for injection and isotonic agents. . Specifically, lactose, saccharose, sorbitol, mannitol, starch, amylopectin, cellulose or gelatin as a binder, dicalcium phosphate as an excipient, corn starch or sweet potato starch as a disintegrating agent, magnesium stearate as a lubricant, Calcium stearate, sodium stearyl fumarate, polyethylene glycol wax, etc. are mentioned.

In addition, the composition for promoting hair growth is indole-3-acetic acid or indole-3-acetic acid-containing composition for promoting hair growth comprising a nano-hybrid complex intercalated between the layered metal salt of the lotion, emulsion, cream, It may be selected from the group consisting of essences or spray formulations, but is not limited to a particular formulation.

Meanwhile, the hair growth promoting effect of the nanohybrid complex according to the present invention can be maximized by the drug delivery property of the layered metal salt, which can be called a carrier of the active substance. The present inventors have disclosed the drug delivery effect of the nanohybrid complex of a layered metal salt and a specific drug in Korean Patent No. 10-523525 (Registration Date 2005. 10. 17). Specifically, in the case of a carrier for delivering a specific drug to the human body, in order to exert its effects or functions, it should be possible to release the active substance at an appropriate rate from the desired tissue, organ or skin. In the case of encapsulation using a conventional polymer, the drug is eluted by the acid addition of the useful drug, whereas in the nanohybrid complex according to the present invention, the drug supported in the layered metal salt is eluted by ion exchange. Therefore, indole-3-acetic acid, an active ingredient of the present invention, also shows an excellent hair growth promoting effect due to the release and transfer properties of the layered metal nanohybrid complex.

Hereinafter, the present invention will be described in more detail with reference to preferred examples. However, the present invention is not limited thereto.

Example 1 Preparation of Nanohybrid Composites (IAA-ZBS) According to the Present Invention

Zn (NO ₃ ) ₂ ㆍ H ₂ O was dissolved in tertiary distilled water from which carbonate ions (CO ₃ ^2- ) had been removed, and then titrated to about 6-7 with 0.2 M NaOH to obtain a zinc basic salt precipitate. . The titrated solution was separated by centrifugation and washed to remove unreacted salts. Thereafter, the prepared layered bilayer zinc basic salt was reacted with indole-3-acetic acid by ion exchange to obtain a precipitate, followed by centrifugation and washing, where indole-3-acetic acid was intercalated between the layers of the layered bilayer zinc basic salt. Organic-inorganic hybrid nanocomposites were prepared. The whole process was carried out under a nitrogen atmosphere to prevent the formation of carbonate ions (CO ₃ ^2- ) by carbon dioxide in the air. The indole-3-acetic acid content in the prepared nanohybrid composite was 42% by weight.

Example 2: Preparation of Emulsion Compositions Containing Nanohybrid Composites (IAA-ZBS)

A composition in the form of an emulsion comprising a nanohybrid composite prepared according to Example 1 was prepared according to the content ratios set forth in Table 1 below. The weight ratio of indole-3-acetic acid to the total weight of the prepared composition was 1% by weight, and the emulsion method used a commonly used O / W method.

ingredient weight % Purified water Balance Butylene glycol 3 Dexpanthenol One Alcohol 5 glycerin 3 Squalane 12.5 Twin 60 2 Stearic acid 1.5 Cetanol 3 Wax 1.5 antiseptic 0.15 Antioxidant 0.1 Spices 0.1 IAA-ZBS prepared in Example 1 2.38 system 100.0

Example 3: Preparation of Pure Indole-3-acetic Acid-Containing Emulsion Composition

Except for using the pure indole-3-acetic acid instead of the nanohybrid composite prepared according to Example 2, a composition for preventing hair loss and promoting hair growth according to the present invention was prepared by the same method as described in Example 2.

Example 4 Preparation of Ethanol Solution Containing Nanohybrid Complex (IAA-ZBS)

In order to confirm the hair growth promoting effect of the nanohybrid complex (IAA-ZBS) other than the emulsion formulation, the solution was prepared so that the content of indole-3-acetic acid was 1% with 30% ethanol as a solvent.

Example 5: Preparation of Pure Indole-3-acetic Acid-Containing Ethanol Solution

In order to confirm the hair growth promoting effect of indole-3-acetic acid itself, a solution was prepared such that the content of indole-3-acetic acid was 1% using 30% ethanol as a solvent.

Test Example 1: Hair growth promoting effect experiment (first animal experiment)

In order to determine the hair growth efficacy of the emulsion preparations prepared according to Examples 2 and 3, the back hair was removed using the mouse (C57BL / 6, M) 6 weeks old (3 mice / group) the next day. From each emulsion formulation was applied to the hair removal site.

On the other hand, as a control, a group to which no substance was applied (Comparative Example 1), a group to which 1% dilution minoxidil was applied (Comparative Example 2), and a commercially available silver nano hair repellent (manufactured by Green of Life, Comparative Example 3) were used. It was. For each experimental group, 100 μl of each formulation was applied for 40 days.

FIG. 1 shows photographs of observing hair regeneration over time of mice to which the formulations according to Examples 2, 3, Comparative Example 1, Comparative Example 2, and Comparative Example 3 were applied.

In addition, the ratio of the hair regeneration portion to the following hair removal portion was calculated and compared, and the evaluation was performed based on the criteria as shown in Table 2 below.

evaluation % New hair growth 3 More than 70% 2 30-70% One Less than 30% 0 Not growing

After 40 days of applying the formulations according to Examples 2 to 3 and Comparative Examples 1 to 3, the average value evaluated according to the criteria of Table 2 was calculated for each experimental group, and the results are shown in Table 3 below. .

division Evaluation (average) 40 days Example 2 IAA-ZBS emulsion application group 3 Example 3 Pure IAA Emulsion Application Group 2 Comparative Example 1 You haven't applied anything 0.33 Comparative Example 2 1% dilution minoxidil application group 2.67 Comparative Example 3 Green O'live application silver nano hair repellent 1.67

As can be seen from the experimental group photograph shown in FIG. 1 and the evaluation average value of Table 3 above, the emulsion comprising the nanohybrid complex (IAA-ZBS) in which the indole-3acetic acid according to the present invention was intercalated between the layered metal salts The composition (Example 2) was able to confirm that the hair growth effect is excellent compared to the case of applying the conventional hair repellent or no formulation. On the other hand, even in the case of emulsion formulation containing pure indole-3-acetic acid (Example 3), the initial hair growth effect after application of the formulation is not very good, but the hair growth photograph after 40 days is significantly superior to other formulations of the comparative example. It showed a hair growth effect. Thereby, the hair growth effect of indole-3-acetic acid itself was also confirmed.

On the other hand, according to the photograph (Comparative Example 2) showing the hair growth rate of the mouse coated with minoxidil (1% dilution solution), which is the most used conventionally, hair gradually grew from 2 weeks, and began to show significant hair growth promoting effect at 4 weeks. Compared with this, the mouse coated with the emulsion formulation of Example 2 according to the present invention showed a hair growth rate that was higher than the hair growth rate of the minoxidil-coated group, and the hair growth site appeared evenly, and thus the hair growth effect of the present invention was very excellent. I could confirm the fact.

Test Example 2: Hair growth promoting effect experiment (second animal experiment)

In order to examine the hair growth efficacy of the indole-3-acetic acid and the nanohybrid complex itself according to Examples 4 to 5, the dorsal site using the mouse (C57BL / 6, M) 6 weeks old (8 mice / group) The hair was removed and each formulation was applied to the hair removal site from the next day.

According to the same method, experiments were conducted in which the various types of drugs or compositions described in Table 2 were applied to mice and observing hair growth, and 100 μl of each formulation was applied for 4 weeks for each experimental group.

division Breakdown Example 4 IAA-ZBS ethanol solution application group Example 5 Pure IAA-containing ethanol solution group Comparative Example 4 ZBS ethanol solution application group without IAA Comparative Example 5 You haven't applied anything Comparative Example 6 1% dilution minoxidil application group

Figure 2 shows a picture of the mouse showing the hair growth rate as a result of applying the formulation of Example 4 to Comparative Example 6 to the mouse.

According to the photograph of FIG. 2, a solution in which the indole-3-acetic acid (Example 5) and the nanohybrid complex according to the present invention was diluted with ethanol (30%) (IAA content: 1% by weight) was applied (Example 4). One mouse showed almost all hair growth at 3 weeks after the application of the emulsion, and at 4 weeks, it was confirmed that the hairs were hardly distinguishable.

In particular, the results of Example 5 were compared with mice coated with ethanol solution containing only layered zinc base salt (ZBS) containing no indole-3-acetic acid (Comparative Example 5) (Comparative Example 5). It shows a distinct hair growth rate, indicating that indole-3-acetic acid itself has an excellent hair growth promoting effect.

1 is an emulsion formulation (Example 2) comprising a nanohybrid complex in which indole-3-acetic acid is intercalated between zinc basic salts according to the present invention, an emulsion formulation containing 1% by weight of pure indole-3-acetic acid (execution) Example 3) When no substance was applied (Comparative Example 1), when minoxidil diluted to 1% by weight was applied (Comparative Example 2), commercially available silver nano hair repellent (manufactured by Green of Life Co., Ltd.) (Comparative Example 3 ) Is a photograph of the mouse showing the effect of promoting hair growth when the respective applications.

FIG. 2 shows the results of a second animal experiment, in which an ethanol (30%) solution containing pure indole-3-acetic acid (Example 4) according to the present invention, indole-3-acetic acid was inserted between layers of zinc basic salts. Ethanol solution containing complex (Example 5), ethanol solution containing zinc basic salt without indole-3-acetic acid (Comparative Example 4), group to which nothing was applied (Comparative Example 5) and 1% dilution Photographs of mouse hair growth of minoxidil-coated group (Comparative Example 6) are shown.

Claims (10)

In the cosmetic composition for preventing hair loss and promoting hair growth comprising a nanohybrid complex in which indole-3-acetic acid is inserted between layers of a layered metal salt, The content of the indole-3-acetic acid is 0.01% to 1% by weight based on the total weight of the composition, The layered metal salt is a cosmetic composition for preventing hair loss and promoting hair growth, characterized in that it is selected from metal layered double hydroxide (LDH: Layered double hydroxide) or zinc basic salt (ZBS) represented by the following formula: LDH: [M ²⁺ _(1-x) N ³⁺ _x (OH) ₂ ] A _x yH ₂ O _{ZBS: Zn 5 (OH) 8} A · nH 2 O or _{Zn 3 (OH) 4 A 2} · nH 2 O Wherein M ²⁺ is a divalent metal cation, N ³⁺ is a trivalent cation, A is indole-3-acetic acid, x is a number ranging from 0.1 to 0.4, y and n are positive integers to be. delete delete delete The method of claim 1, M ²⁺ is selected from the group consisting of Mg ²⁺ , Ni ²⁺ , Cu ²⁺ and Zn ²⁺ , and N ³⁺ is Al ³⁺ , Fe ³⁺ , V ³⁺ , Ti ³⁺ and Ga ³⁺ Hair loss prevention and hair growth promoting cosmetic composition, characterized in that selected from the group consisting of. The method of claim 1, The nanohybrid composite is obtained by dissolving Zn (NO ₃ ) ₂ .H ₂ O in distilled water, and then titrating the pH to 6-7 to obtain a zinc base salt precipitate, and reacting indole-3-acetic acid by ion exchange. Cosmetic composition for preventing hair loss and promoting hair growth characterized in that it is prepared through the separation and washing process. The method of claim 6, The distilled water is a cosmetic composition for preventing hair loss and promoting hair growth, characterized in that distilled water from which carbonate ions (CO ₃ ^2- ) has been removed. delete delete The method of claim 1, The hair loss prevention and hair growth promoting cosmetic composition is a cosmetic composition for preventing hair loss and promoting hair growth, characterized in that selected from the group consisting of lotions, emulsions, creams, essences or spray formulations.

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