KR101059282B1 - Composition for the prevention and treatment of drug addiction and withdrawal symptoms containing gold and silver extract as an active ingredient - Google Patents

Abstract

The present invention relates to a composition containing the sterling silver extract as an active ingredient, specifically, the sterling silver extract of the present invention, as well as the effect of reducing locomotor activity used as an indicator of drug addiction, in the nucleus and striatum of the brain By confirming the rapid reduction of c-Fos expression, which is an indicator of neuronal activity, the composition may be usefully used as a pharmaceutical composition or health functional food for the prevention and treatment of drug addiction and withdrawal symptoms.

Gold Silver Coins, Gait Activity, c-Fos, Cocaine, Withdrawal Symptoms

Description

A composition comprising the extract of Lonicera japonica Thunberg for preventing and treating drug intoxication or withdrawal symptoms}

The present invention relates to a pharmaceutical composition or health functional food for the prevention and treatment of drug addiction and withdrawal symptoms containing gold and silver extract as an active ingredient.

Einhorn, LC, et al., Electrophysiological effect of cocaine in the mesoaccumbens dopamine system: studies in the ventral tegmental area, J Neurosci., 8 (1) , pp 100-112, 1988

2 Wiechman BE., Et al., Pharmacol. Biochem. Behav ., 15 (3) , pp. 425-433, 1981

Parada A., et al., Neuropharmacology , 39 (9) , pp. 1645-1652, 2000

4 Pontieri FE., Et al., Natl. Acad. Sci. USA. , 92 , pp.12304-12308, 1995

Kuczenski R., et al., J. Neurosci ., 11 (9) , pp.2703-2712, 1991

[Document 6] Park, Jong-Hee Author, Encyclopedia of Herbal Medicine (Ha), Book Publishing Company, Il-il, p.654-655, 2002

[Reference 7] Moon Tae-Chul et al. 7, Anti-inflammatory activity of gold and silver flavonoids, Korean Journal of Pharmacy, Journal of Pharmacy, 43 (1) , pp.117-123, 1999

[Document 8] Park, Uk-Yeon et al. 2, Antimicrobial Effect of Medicinal Herb Extracts, Korean Society of Food Science and Nutrition, Korean Society of Food Science and Nutrition, 21 (1) , pp.91-96, 1992

9, Karasinska JM., Et al., Eur Neurosci ., 22 (7) , pp.1741-1750, 2005

10 Jang EY, et al., Eur J Pharmacol., 587 (1-3) , pp. 124-128, 2008

[Reference Document 11] Hyung-Chul Lee et al., Journal of Oriental Pathology , 15 (4) , pp543-547, 2001

The spread of drug addiction is one of the most important modern social problems due to the development of scientific civilization and the stress of modern people due to the rapid economic society. Drug refers to a substance used to prevent or treat a disease, and refers to a substance that causes a change in physical function. Abuse of these drugs raises the problem of self-destruction both physically, mentally and socially on the negative side. Drug addiction refers to a physical reaction to drugs such as opiates, neurostabilizers or alcohol. Drug addiction has symptoms of tolerance, withdrawal symptoms and habituation, which can mean loss of control due to drug use. Recent drug abuse is one of the world's most serious mental illnesses caused by the increasing number of adolescents and female drug abusers. The development of drugs for addictive drugs is urgently needed.

As a solution of drug addiction, various social policies, treatments, and rehabilitation programs are being implemented, but they have limitations as a fundamental treatment measure.

Addictive drugs such as cocaine, amphetamine, morphine, nicotine, cannabis, caffeine, etc., cause behavioral sensitization in behavior. Repeated or intermittent doses of an addictive drug gradually increase locomotor activity and homotypy, a developmental phase in which a gradual increase in activity is induced. of sensitization and the expression of sensitization, which is a state where activity is developed for a relatively long time, and the neural substrate responsible for this phenomenon is the mesolimbic dopamine pathway in the central nervous system. Dopamine neurons and their target regions, the nucleus accumbens (NAc) and striatum. (Einhorn, LC, et al., Electrophysiological effect of cocaine in the mesoaccumbens dopamine system: studies in the ventral tegmental area, J Neurosci., 8 (1) , pp 100-112, 1988). It is known to increase the amount of walking activity following administration (Wiechman BE., Et al., Pharmacol. Biochem. Behav ., 15 (3) , pp.425-433, 1981). Is being used.

Cocaine is induced through drug-intensifying effects. The factor affecting this behavioral change is the activation of the dopamine (DA) neurotransmitter. In particular, cocaine administration compensates and strengthens the ventral tegmental area. The midbrain limbic system, originating in the A10 neuron of the area (VTA) and projecting into the nucleus accumbens, is known to play an important role (Einhorn, LC, et al., Electrophysiological effect of cocaine in the mesoaccumbens dopamine system). : studies in the ventral tegmental area, J Neurosci., 8 (1) , pp 100-112, 1988).

In a recent study due to the administration of cocaine, known as indicators of neuronal activity in drug addiction and related cheukhaek and projected areas of the striatum, including dopaminergic neurons of the early gene cF os (Immediate early gene cF os ) protein, c of There are reports of increased fos expression. Repeated administration of SKF-38393, a dopamine D1 receptor agonist, to experimental animals increased the expression of c-Fos, an indicator of gait activity and neuronal activity. In addition, the administration of dopamine antagonist SCH 23390 resulted in a decrease in ambulatory activity caused by cocaine (Parada A., et al., Neuropharmacology , 39 (9) , pp.1645-1652, 2000). In addition, in the experiments observed using in vivo microdialysis, the concentration of dopamine due to cocaine administration in the striatum and the nucleus, which are related to drug intoxication, increased significantly (Pontieri FE., Et. al., Natl. Acad. Sci. USA. , 92 , pp . 12304-12308, 1995), which showed an increase in gait activity, correlating dopamine, a biochemical aspect, and gait activity, a behavioral aspect. Experimental evidence has been shown to show that there is (Kuczenski R., et al., J. Neurosci ., 11 (9) , pp.2703-2712, 1991).

Therefore, the identification of neural mechanisms involved in drug addiction may be the ultimate solution to drug addiction treatment.

Lonicerae Caulis et Folium is a dry stem or lobe of Lonicera japonica Thunberg, which is attached because the vine does not live and wither in cold winter.

In general, gold and silver coins represent the bud part of the genus Phosphorus or its variety, and both the stem, the leaves, and the flowers are used for the herbaceous cortex. However, clinically, flowers (gold and silver) are more effective than 藤, 藤 (leaf), so now they mostly use only flowers. The name of gold and silver is the name of the first blooming flower, but gradually changed to yellow. Inhwahwa, Ewha, Silver, Ssanghwa, Geumhwa, Geumdeunghwa, Geumeundeung, Mandarin Lamp Various names such as 藤, Rosa lanterns, Noh Onsu, left front lantern, Geumchaego, Tongyeongcho and Tongtong, etc. It has a peculiar smell and tastes sweet and cold. It contains about 1% of luteolin and inositol, and contains saponin, tannin, and lonicerin (luteolinrhamnoglucoside). (Bottom), Shinil Trading Co., p.654-655, 2002), the effect is dysentery, skin tissue necrosis due to heat poisoning, mastitis, colitis, gastric ulcer, cystitis, sore throat, tonsillitis, bronchitis, conjunctivitis and mumps, mumps It has high fever and purulent infectious diseases, and its pharmacological effects are antimicrobial, anti-inflammatory, antipyretic, leukocyte phagocytosis, central nervous system excitability, serum cholesterol drop, and ulcer prevention effects (Mt. the anti-inflammatory effect of flavonoid components honeysuckle, of Pharmacy, about Journal, 43 (1), pp.117-123, 1999; antimicrobial effect of the two, other medicinal herb extracts bakukyeon search, Korea Food Research, Korean Journal of Nutrition, Food Science and Nutrition Korea, 21 (1), pp.91-96, 1992), supra anywhere honeysuckle extract drug addiction and also not been any initiation of effective withdrawal symptoms.

Therefore, the present inventors confirmed that gold and silver extract extracts not only the effect of reducing the amount of gait activity used as an indicator of drug addiction, but also dramatically decreases c-Fos expression, which is an indicator of neuronal activity in the nucleus and striatum of the brain. The present invention was completed by confirming the therapeutic effect of the symptoms.

In order to achieve the above object, it provides a pharmaceutical composition for the prevention and treatment of drug addiction and withdrawal symptoms containing a gold coin extract as an active ingredient.

In another aspect, the present invention provides a dietary supplement for the prevention and improvement of drug addiction and withdrawal symptoms containing gold and silver extract as an active ingredient.

The sterling silver extracts as defined herein are water, lower alcohol solvents of C ₁ to C ₄ or mixed solvents thereof, preferably water and ethanol mixed solvents, more preferably 60 to 100% ethanol mixed solvents, most preferably 90 To extracts soluble in 100% ethanol.

Drugs as defined herein are cocaine, amphetamine, morphine, nicotine, cannabis or caffeine, and the like, preferably characterized as being cocaine.

Hereinafter, the present invention will be described in detail.

The gold coin extract of the present invention can be prepared as follows.

After drying and crushing the gold and silver coins of the present invention, about 1 to 25 times, preferably about 5 to 20 times the amount of water, C ₁ to C ₄ lower alcohol solvents and mixed solvents thereof, Preferably hot water extraction, cold extraction, reflux cooling extraction or ultrasonic extraction with water and ethanol mixed solvent, more preferably 60-100% ethanol mixed solvent at room temperature for about 10-96 hours, preferably 60-80 hours Using an extraction method such as, preferably, the extract obtained after cold extraction is filtered, concentrated under reduced pressure or dried to obtain the gold coin extract of the present invention.

The present invention provides a pharmaceutical composition for the prevention and treatment of drug addiction and withdrawal symptom, which contains the gold and silver extract obtained by the manufacturing method as an active ingredient.

The composition of the present invention comprises from 0.02 to 50% by weight of the extract relative to the total weight of the composition.

However, the composition is not limited thereto, and may vary depending on the condition of the patient, the type of disease, and the progress of the disease.

The composition containing the gold coin extract of the present invention may further comprise suitable carriers, excipients and diluents commonly used in the manufacture of pharmaceutical compositions.

Compositions comprising extracts according to the invention are formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols and the like, oral preparations, suppositories and sterile injectable solutions, respectively, according to conventional methods. Carriers, excipients and diluents which may be used in combination with the extract, and which may be included in the composition comprising the extract include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin , Calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzate, talc, magnesium stearate and mineral oil. When formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, and surfactants are usually used. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and the solid preparations may include at least one excipient such as starch, calcium carbonate, sucrose in the extract. ) Or lactose, gelatin and the like are mixed. In addition to simple excipients, lubricants such as magnesium styrate talc are also used. Examples of the liquid preparation for oral use include suspensions, solutions, emulsions, and syrups. In addition to water and liquid paraffin, simple diluents commonly used, various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included . Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like can be used. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.

Preferred dosages of the extracts of the present invention vary depending on the condition and weight of the patient, the extent of the disease, the form of the drug, the route of administration and the duration, and may be appropriately selected by those skilled in the art. However, for the desired effect, the extract of the present invention is preferably administered at 0.01 mg / kg to 10 g / kg, preferably 1 mg / kg to 1 g / kg per day. The administration may be carried out once a day or divided into several doses. Therefore, the above dosage does not limit the scope of the present invention in any aspect.

The composition of the present invention may be administered to mammals such as rats, mice, livestock, humans, and the like in various routes. All modes of administration can be expected, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intrauterine dural or Intracerebroventricular injection.

The present invention also provides a dietary supplement for the prevention and improvement of drug addiction and withdrawal symptoms containing gold and silver extract as an active ingredient.

As defined herein, "health functional food" means a food manufactured and processed using raw materials or ingredients having functional properties useful for the human body under the Health Functional Food Act No. 6767, and "functional" means It means ingestion for the purpose of obtaining useful effects on health use such as nutrient control or physiological action on structure and function.

The composition comprising the extract of the present invention can be used in various ways, such as drugs, foods and drinks for the prevention and improvement of drug addiction and withdrawal symptoms. Foods to which the gold and silver extract of the present invention may be added include, for example, various foods, beverages, gums, teas, vitamin complexes, health supplements, and the like, and are powders, granules, tablets, capsules, syrups or beverages. Can be used as

The gold and silver extract of the present invention has little toxicity and side effects, and thus is a drug that can be used safely even when taken for long periods of time.

The extract of the present invention may be added to food or beverage for the purpose of preventing and treating drug addiction and withdrawal symptoms. At this time, the amount of the extract in the food or beverage is generally added to the health food composition of the present invention to 0.01 to 15% by weight of the total food weight, the health beverage composition is 0.02 to 10 g based on 100 ml, preferably Can be added in a ratio of 0.3 to 1 g.

In addition to containing the extract as an essential ingredient in the indicated ratio, the health beverage composition of the present invention is not particularly limited in the liquid component and may contain various flavors or natural carbohydrates as additional ingredients, as in general beverages. Examples of the above-mentioned natural carbohydrates include monosaccharides such as disaccharides such as glucose and fructose such as maltose, sucrose and the like and polysaccharides such as dextrin, cyclodextrin and the like Sugar, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (tauumatin, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The proportion of natural carbohydrates is generally about 1-20 g, preferably about 5-12 g per 100 ml of the composition of the present invention.

In addition to the above, the composition of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, coloring and neutralizing agents (such as cheese and chocolate), pectic acid and salts thereof, alginic acid and its Salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. The compositions of the present invention may also contain pulp for the production of natural fruit juices and fruit juice beverages and vegetable beverages. These components can be used independently or in combination. The proportion of such additives is not so critical, but is generally selected in the range of 0 to about 20 parts by weight per 100 parts by weight of the composition of the present invention.

The gold and silver extract of the present invention not only reduces the amount of gait activity used as an indicator of drug addiction, but also shows an effect of rapidly decreasing c-Fos expression, which is an indicator of neuronal activity in the nucleus and striatum of the brain, drug addiction and withdrawal. It can be usefully used as a pharmaceutical composition or health functional food for the prevention and treatment of symptoms.

Hereinafter, the present invention will be described in detail by the following reference examples, examples and experimental examples.

However, the following reference examples, examples and experimental examples are merely to illustrate the invention, the content of the present invention is not limited by the following reference examples, examples and experimental examples.

Reference Example 1. Preparation of Laboratory Animals

The experimental animals were Sprague-Dawley male rats (Hyochang Science) weighing 250-260 g. In the time-contrast cycle, free intake of feed and negative water was allowed, and sufficient water and food were provided before the start of the experiment, and the animals were handled for 10 minutes a day for 3 days before the start of the experiment.

Reference Example 2. Statistical processing

Pedestrian activity was measured by horizontal movement distance (cm) in the box, and statistical significance was tested by the SPSS program (Version 11.01) post hoc Turkey test. Admitted.

Example 1 Preparation of Gold Coin Extract

Purified from Daewon Pharmaceutical Co., Ltd. located in Daegu Metropolitan City, 3 kg of finely divided gold and silver coins were added with 15 L of 95% ethanol, and the extract was extracted for 72 hours at room temperature (24 ° C), and then filtered under reduced pressure (EYELA Co., Concentration under reduced pressure in Japan) to give 290 g of silver coin extract (hereinafter referred to as "LJ").

Experimental Example 1.Effect of Gold Coin Extract on Behavioral Sensitivity after Drug Administration

In order to confirm the effect of the sterling silver extract of Example 1 on the behavioral sensitivity due to acute and chronic drug administration, the experiment on the change in gait activity of rats after cocaine administration was applied as follows. The same method was performed (Karasinska JM., Et al., Eur Neurosci ., 22 (7) , pp.1741-1750, 2005).

1-1. How to measure amount of walking activity

The activity of the experimental animals was measured by an etovision program (Ethovision program, Noldus Information Technology BV, Wageningen, Netherlands) using videotracking. In detail, the horizontal movement of the test animal was tracked using a black matt acrylic box of 40 cm x 40 cm x 45 cm in width, length, and height, and the image obtained from the digital camera was installed on about 2.5 m of nine boxes. By using a contrasting principle of a white subject on a black background, it transfers it to a computer and recognizes the center point of the white experimental image several times per second. Phosphorus distance was quantified.

1-2. Measurement of ambulatory activity

The experimental animals prepared in Reference Example 1 were transferred to a laboratory where external noise was blocked in a kennel and weighed, respectively, and then placed in nine activity measuring boxes individually. After measuring baseline for 60 minutes in a box after 60 minutes of adaptation time, 25, 50, and 100 mg / kg of gold and silver extracts were orally administered once, and 2 ml / kg of vehicle (70) was used as a control. % PEG + 1% Tween 80 + 10% EtOH) was administered orally. Vehicle or gold silver extract was orally administered, after 60 minutes physiological saline or cocaine 20 mg / kg single dose, and walked for an additional 60 minutes to measure the amount of walking activity in the horizontal moving distance (cm) in the box.

As a result of the experiment, as shown in Table 1 and Figure 1, in the normal group (physiological saline treatment group after the vehicle administration) to 1101.10 ± 305.47 cm, increased to 20671.56 ± 1876.09 cm in the control group (cocaine treatment group after vehicle administration) On the contrary, in the 25, 50 and 100 mg / kg administration group, the amount of gait activity decreased compared to the control group, increasing to 9547.57 ± 1692.65 cm, 11808.75 ± 2154.74 cm and 5884.67 ± 1227.53 cm, respectively. It was confirmed that the mg / kg administration group was significantly reduced compared to the control group (see Table 1 and Figure 1).

In addition, as shown in FIG. 2 or Table 1, in order to check whether behavioral change occurs after administration of the gold leaf extract in a normal state, oral administration of 100 mg / kg of the gold leaf extract (gold leaf alone administration group), After 60 minutes of treatment, walking activity was measured for 1419.03 ± 609.38 cm, which was similar to that of the normal group.

Experimental group Treatment contents After cocaine administration
1 hour gait activity (cm) Normal Saline treatment after vehicle administration 1101.10 ± 305.47 Control group cocaine treatment after vehicle administration 20671.56 ± 1876.09 Gold and Silver Coins 25 mg / kg Cocaine treatment after 25 mg / kg gold 9547.57 ± 1692.65 Gold Silver Coins 50 mg / kg Cocaine treatment after 50 mg / kg gold 11808.75 ± 2154.74 Gold Silver Coins 100 mg / kg Cocaine treatment after 100 mg / kg gold 5884.67 ± 1227.53 Gold and silver coin alone group Physiological saline treatment after administration of gold and silver 100 mg / kg 1419.03 ± 609.38

Experimental Example 2. Effect of sterling silver extract on c-Fos expression by immunohistochemical analysis by drug administration

In order to confirm the expression of the initial gene c-fos by drug administration of the sterling silver extract obtained in Example 1, the following experiment was applied by applying the method described in the existing literature (Jang EY, et al., Eur J Pharmacol., 587 (1-3) , pp.124-128, 2008)

On the day of the test, 100 mg / kg of gold and silver extract was orally administered, and after 1 hour, 20 mg / kg of cocaine was intraperitoneally administered, followed by anesthesia with sodium phentobarbital (100 mg / kg) after 2 hours. The brain tissue was fixed by perfusion with 500 ml of 4% paraformaldehyde (PFA). After removing the brain tissue, it was fixed in 10% sucrose / 4% PFA for about 2 hours, soaked in 20% sucrose / phosphate buffer solution (PBS) solution and refrigerated at 4 ° C for one day. Maintained. Then, the brain tissue was sectioned at 30 um with cryotome (Shandon Cryotome, Thermo Fisher Scientific) at -20 ° C, and the nucleus site was taken and placed in 0.1% sodium azide / PBS. Stored at ° C.

Tissue sections were washed three times in buffer (PBS) to remove lipids from cell membranes for 2 minutes in 2% Triton-X-100 / PBS solution and then blocked with 3% BSA / PBS solution. After reacting with a primary rabbit anti-Fos antibody (Santa cruz, CA, USA) diluted 1: 1000 in 0.1% BSA / PBS solution at 4 ° C. for 20 hours, c- using DAB as a colorant was used. Fos protein was expressed.

As a result, as shown in FIG. 3, the group treated with 100 mg / kg of gold and silver extracts significantly reduced the expression of the c-Fos gene, which is a neuronal activity marker, in the nucleus and striatum compared to the control group (FIG. 3). 3).

Experimental Example 3. Acute Toxicity Test

In order to confirm the toxicity by the administration of the sterling silver extract obtained in Example 1, the following experiment was applied by applying the method described in the existing literature (Hyung-Chul Lee et al., Journal of Oriental Pathology , 15 (4) , pp543-547, 2001) .

Acute toxicity test was performed using 6-week-old specific pathogen-free (SPF) SD rats. Two animals of each group were orally administered with the gold coin extract of the present invention at a dose of 500 mg / kg. After administration of the test substance, mortality, clinical symptoms, and changes in body weight were observed, and hematological and hematological examinations were performed.

As a result, no significant clinical symptoms or dead animals were noted in all animals treated with the test substance, and no toxic changes were observed in weight changes, blood tests, blood biochemical tests, and autopsy findings.

In addition, the gold and silver extract of the present invention did not show toxic changes up to 500 mg / kg in rats, respectively, and the minimum lethal dose (LD ₅₀ ) was determined to be a safe substance of 500 mg / kg or more.

Hereinafter, an example of the preparation of the composition containing the gold coin extract of the present invention, but the present invention is not intended to limit it, but is intended to explain in detail only.

Formulation Example 1 Preparation of Powder

Gold Coin Extract (LJ) 20 mg

Lactose 100 mg

Talc 10 mg

The above ingredients are mixed and filled in an airtight cloth to prepare a powder.

Formulation Example 2 Preparation of Tablet

Gold Coin Extract (LJ) 10 mg

Corn starch 100 mg

Lactose 100 mg

Magnesium stearate 2 mg

After mixing the above components and tableting according to the conventional tablet manufacturing method to prepare a tablet.

Formulation Example 3 Preparation of Capsule

Gold Coin Extract (LJ) 10 mg

3 mg of crystalline cellulose

Lactose 14.8 mg

Magnesium Stearate 0.2 mg

According to a conventional capsule preparation method, the above ingredients are mixed and filled into gelatin capsules to prepare capsules.

Formulation Example 4 Preparation of Injection

Gold Coin Extract (LJ) 10 mg

180 mg mannitol

Sterile sterilized water for injection 2974 mg

Na2HPO4,12H2O 26 mg

According to the conventional method for preparing an injection, the amount of the above ingredient is prepared per ampoule (2 ml).

Formulation Example 5 Preparation of Liquid

Gold Coin Extract (LJ) 20 mg

10 g of isomerized sugar

5 g of mannitol

Purified water

After dissolving each component in purified water according to the usual method of preparing a liquid solution, adding lemon flavor appropriately, mixing the above components, adding purified water, adjusting the whole to 100 ml by adding purified water, and then filling into a brown bottle. The solution is prepared by sterilization.

Formulation Example 6 Preparation of Healthy Food

Gold Silver Coconut Extract (LJ) 1000 mg

Vitamin mixture proper amount

70 μg of Vitamin A Acetate

Vitamin E 1.0 mg

Vitamin B1 0.13 mg

Vitamin B2 0.15 mg

Vitamin B6 0.5 mg

0.2 μg of vitamin B12

Vitamin C 10 mg

10 μg biotin

Nicotinic Acid 1.7 mg

50 μg folic acid

Calcium Pantothenate 0.5mg

Mineral mixture quantity

Ferrous Sulfate 1.75 mg

0.82 mg of zinc oxide

Magnesium carbonate 25.3 mg

15 mg of potassium phosphate monobasic

Secondary calcium phosphate 55 mg

Potassium citrate 90 mg

100 mg of calcium carbonate

Magnesium chloride 24.8 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixture is comparatively mixed with a composition suitable for health food as a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional method for producing healthy foods , Granules can be prepared and used in the manufacture of health food compositions according to conventional methods.

Formulation Example 7 Preparation of Healthy Drink

Gold Silver Coconut Extract (LJ) 1000 mg

Citric acid 1000 mg

100 g of oligosaccharide

Plum concentrate 2 g

Taurine 1 g

Purified water was added to a total of 900 ml

The above components were mixed according to a conventional health drink manufacturing method, and the mixture was heated at 85 DEG C for about 1 hour with stirring, and the solution thus prepared was filtered to obtain a sterilized 2-liter container, which was sealed and sterilized, ≪ / RTI >

Although the composition ratio is a mixture of the components suitable for the preferred beverage as a preferred embodiment, the blending ratio may be arbitrarily varied according to the regional and national preferences such as the demand level, the demanding country, and the intended use.

[National R & D project supporting this invention]

[Task unique number]

       515-82-06593

[Name of Buddha]

       Ministry of Knowledge Economy

[Name of research project]

       Regional Innovation Center Project

[Name of Research Project]

       Development of Herbal Medicine and Natural Products for Treatment of Brain Disease

[Host]

        Daegu Haany University Industry-Academic Cooperation Foundation

[Research period]

        March 01, 2008 to February 28, 2009

1 is a diagram showing the effect on the amount of gait activity of the gold coin extract induced by cocaine administration,

2 is a view showing the effect on the amount of gait activity when administration of gold and silver coin extract (100 mg / kg),

3 is a diagram showing the effect on the expression of c-Fos in the nucleus and striatum of the cerebral nucleus extract induced by cocaine administration.

Claims (6)

Gold and silver coins 60-100% pharmaceutical composition for the prevention and treatment of cocaine poisoning containing water and ethanol mixed solvent extract as an active ingredient. delete delete delete Gold and silver functional food for the prevention and improvement of cocaine poisoning containing 60 to 100% water and ethanol mixed solvent extract as an active ingredient. delete

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