KR101000953B1 - EXTRACTS OF Lespedeza cuneate G. Don AND THEIR USES - Google Patents

Abstract

The present invention relates to a non-repair ( Lespedeza cuneate G. Don) extract and its use, specifically, the present invention is a non-repair extract having anticancer activity extracted by adding water or alcohol to the non-repair and a pharmaceutical containing the non-repair extract as an active ingredient To compositions and functional foods.

The non-repair extract according to the present invention has anticancer activity and can be usefully prepared by preparing a pharmaceutical composition and a functional food for an anticancer agent containing it as an active ingredient because there is no cytotoxicity.

Non-repair extract, anticancer, pharmaceutical composition, functional food

Description

Non-repair extracts and uses thereof {EXTRACTS OF Lespedeza cuneate G. Don AND THEIR USES}             

Figure 1 shows the process of performing the MTT analysis in Experiment 2.

The present invention relates to a non-repair ( Lespedeza cuneate G. Don) extract and its use, specifically the present invention is a non-repair extract extracted by the addition of water or alcohol to the non-repair and anti-cancer pharmaceuticals containing the non-repair extract as an active ingredient A composition and a functional food.

Cancer treatment is basically performed by surgical therapy, chemotherapy, radiation therapy, and additionally by topical therapy, hormone therapy, photodynamic therapy, and laser therapy. Recently, immunotherapy and gene therapy have also been attempted. However, these treatments facilitate cancer treatment, but increased the cancer recurrence rate even after the cancer treatment was completed, and caused side effects by treating the cancer cells and inducing cytotoxicity against normal cells.

In order to overcome this problem, researches are being conducted to separate substances from natural products that have anticancer activity and are not toxic to normal cells.

As an example of the study, mushrooms and garlic, such as fingering, ganoderma, and circumstance, are known to contain immune enhancing substances that enhance biodefense ability ( Appl Microbiol Biotechnol, 2002, 60 , 258-274; Urology, 2002 , 60 , 640-644), chlorophyllol, vinegar, and non-leaflets contain urson, which inhibits the growth of cancer cells by inhibiting neovascularization ( J Ethnopharmacol, 1995, 49 , 57-68). Is known to contain a substance that is toxic to cancer cells and causes cell death (US Pat. No. 5869060, 1999). In addition, emodin in aloe, indole in cruciferous plants, and berberine in yellow-white are known as substances which inhibit abnormal proliferation of cancer cells ( Cancer Lett , 2002, 28 , 135-139, 189; Cancer Lett , 2000, 148 , 19-25).

The inventors studied non-repair extracts as an example of such a study.

Beesuri is a perennial herbaceous plant belonging to the legume family, and is distributed throughout Korea, Japan, Taiwan, China, India, and Australia, and has excellent viability. This non-aurius is used as a herbal medicine, specifically, it is used to treat old people's lack of nourishment, impotence, premature ejaculation, cough, asthma, diabetes, and nervous breakdown. ).

It is an object of the present invention to obtain an extract from non-repair, the extract has anti-cancer activity, it is found that there is no cytotoxicity non-repair extract having anti-cancer activity and the pharmaceutical composition and anti-cancer pharmaceutical composition containing the same as an active ingredient and functional food To provide.

In order to achieve the above object, the present invention provides a non-repairing extract.

The present invention also provides a pharmaceutical composition and a functional food for anticancer drugs containing the non-repair extract as an active ingredient.

Hereinafter, the present invention will be described in detail.

The present invention provides a non-repair extract.

Specifically, the present invention provides a non-repair extract extracted by adding water to the non-repair.

The non-repair extract is

1) adding water to the non-repair, hot water extraction, and filtering to prepare an extraction filtrate; (step 1) and                     

2) The extraction filtrate is prepared through the step of concentrating under reduced pressure and lyophilizing to prepare a non-repair extract (step 2).

1) Step 1

Step 1 is a step of adding water to the fine non-repair, hot water extraction, and then filtered to prepare an extraction filtrate. Hot water extraction is for eluting the active ingredient contained in the non-repair by adding to the water and heating the fine non-repair, it is possible to increase the dissolution rate of the active ingredient contained in the non-repair. Preferably, the hydrothermal extraction is performed for 3 hours at a temperature of 100 ℃ while mixing and stirring the fine non-repair and water. For non-repair, water can be changed according to the hydrothermal extraction conditions, preferably 10 to 20 liters of water per kg of non-repair. In addition, the extraction efficiency can be improved by repeating the hot water extraction three times or more.

2) step 2

Step 2 is a step of preparing a non-aqueous extract by concentrating the extract filtrate prepared in step 1 under reduced pressure and freeze-drying. The vacuum concentration and freeze-drying may be carried out in a conventional manner.

 In addition, the present invention provides a non-repair extract extracted by adding alcohol to the non-repair.                     

The non-repair extract is

1) adding alcohol to the non-repair, hot water extraction, and filtering to prepare an extraction filtrate; (step 1) and

2) The extraction filtrate is prepared through the step of concentrating under reduced pressure and lyophilizing to prepare a non-repair extract (step 2).

1) Step 1

Step 1 is a step of preparing an extraction filtrate by adding alcohol to non-repair, hot water extraction, and then filtered. Alcohol is added to the fine non-repair, and hydrothermal extraction for 3 hours at 70 ℃. The alcohol is preferably used ethanol. For non-repair the alcohol can be changed according to the hydrothermal extraction conditions, preferably 10-20 liters of alcohol per kg of non-repair. In addition, the extraction efficiency can be improved by repeating the hot water extraction three or more times.

2) step 2

Step 2 is a step of preparing a non-aqueous extract by concentrating the extract filtrate prepared in step 1 under reduced pressure and freeze-drying. The vacuum concentration and freeze-drying may be carried out in a conventional manner.

In the present invention, it was found that the non-repair extract extracted by adding water or alcohol to the non-repair has anti-cancer activity and is not cytotoxic.

The present invention also provides a pharmaceutical composition for an anticancer agent containing the non-repair extract having the anticancer activity as an active ingredient.

The pharmaceutical composition for anticancer drugs of the present invention contains the non-repair extract as an active ingredient. In general, in medicine, the effective dose of the non-repair extract of the present invention is 200-1200 mg / day for adults, and can be administered 1 to 4 times a day. However, the dosage can be varied, in particular, taking into account the constitution specificity and weight of the subject to be treated, the type and depth of the disease, the nature of the formulation, the nature of the drug administration and the duration or interval of administration.

The non-repair extract may be administered orally or parenterally during clinical administration and may be used in the form of a general pharmaceutical preparation. Specifically, the non-repair extract of the present invention can be administered in various oral and parenteral dosage forms in actual clinical administration, and when formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, and surfactants are commonly used. It is prepared using.

Solid preparations for oral administration include tablets, pills, powders, granules and capsules, and the like, which include at least one excipient such as potato starch, calcium carbonate, sucrose, colloidal silicic acid, It is prepared by mixing lactose and gelatin. In addition to simple excipients, glidants such as magnesium stearate, talc and talc are also used.

Liquid preparations for oral administration include suspensions, solvents, emulsions and syrups, and such liquid preparations may include various diluents, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to simple diluents, liquid paraffin, etc. have.

Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized formulations and suppositories. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oils such as olive oil, injectable esters such as ethyl oleate and the like can be used. As the base of the suppository, uthepsol, macrogol, tween 61, cacao butter, laurin butter, glycerol, gelatin and the like can be used.

In addition, the present invention provides a functional food having an anticancer activity containing the non-repair extract with anticancer activity as an active ingredient.

The dietary supplement of the present invention contains the non-repair extract as an active ingredient, and there is no particular limitation on the functional food. Typical drinks include alcoholic beverages, alcoholic beverages, juice, jelly, and gum.

When the non-repair extract of the present invention is added to a functional food, the non-repair extract may be added as it is or used with other foods or food ingredients, and may be appropriately used according to a conventional method. The mixing amount of the non-repair extract can be appropriately determined depending on the purpose of use thereof. In general, in the preparation of food or beverage, the non-repair extract of the present invention is added in an amount of 0.1 to 5% by weight based on the raw materials.

Hereinafter, the present invention will be described in detail with reference to examples.                     

However, the following examples are merely to illustrate the invention, but the content of the present invention is not limited by the following examples.

Example 1 Preparation of Non-Repairing Extract 1

1.2 kg of fine non-repair was placed in a water heater (Hanil Engineering, Korea) and 12 liters of water was added thereto, followed by extraction of hot water at 100 ° C. for 3 hours. After repeating the above procedure three times, the resulting solution was filtered to prepare an extract filtrate. The extract filtrate was concentrated under reduced pressure and freeze-dried to obtain 282.1 g (yield: 23.4%) of a non-repair extract in a solid state.

Example 2 Preparation of Non-Repairing Extract 2

1.2 kg of fine non-repair was put in a water heater (Hanil Engineering, Korea) and 12 liters of 70% ethanol was added, followed by hot water extraction at 70 ° C. for 3 hours. After repeating the above procedure three times, the resulting solution was filtered to prepare an extract filtrate. The extract filtrate was concentrated under reduced pressure and freeze-dried to obtain 352.6 g (yield: 29.3%) of non-repair extract in solid state.

Experimental Example 1 Anticancer Effect Analysis Experiment

In Experimental Example 1, in order to determine the anticancer effect of the non-repairing extract according to the present invention, the non-repairing extracts prepared in Examples 1 and 2 were orally administered to experimental male mice transplanted with solid cancer cells, and then the weight of male mice. And the volume of solid cancer cells was measured.                     

(1) experimental animals

Five-week-old male mice (BDF1-based) purchased from Japan SLC were adapted for at least seven days and then used in the experiment. During the adaptation period and the experimental period, the male male mice were separated and housed in a polycarbonate breeding box for rats, and the feed and negative water were freely ingested. During the adaptation and experiment period of the male male experiment, the temperature was 23 ± 2 ℃, the humidity is 55 ± 15%, the ventilation number is 15 to 20 times / hour, the illumination time was 12 hours.

(2) Transplantation of solid cancer cell sections into male mice and oral administration of non-repaired extracts

In order to transplant the solid cancer cell sections into the male laboratory mice, the solid cancer cell sections to be transplanted were prepared by the following method.

Solid tumor cells B16 melanoma tumor (B16 melanoma tumor mass, Cell Line Bank, Seoul National University) stored at −70 ° C. were thawed at 37 ° C., and then subcutaneously administered to the experimental male mouse fluid and site. . When the solid cancer cells of the experimental male mice implanted with the solid cancer cells were 10 × 10 × 10 mm or more, the solid cancer cells were extracted, washed with physiological saline, and a solid cancer cell slice having a size of 2 × 2 × 2 mm was prepared.

In addition, the non-repair extract prepared in Example 1 was suspended in 1% hydroxypropylmethylcellulose (hereinafter referred to as "HPMC") to prepare a test material.

The prepared solid cancer cell sections were implanted subcutaneously into the experimental male mouse fluid and the site, and the test substance was orally administered. At this time, in order to determine the anticancer effect according to the oral administration dose of the non-repair extract, the experiment was divided into a low dose group and a high dose group, and 6 male mice were used for each group.

The dose was 100 mg / kg for the low dose group and 300 mg / kg for the high dose group, and the interval and duration of administration were 2 / day and 17 days. And the administration liquid amount was 5 ml / kg.

The non-repair extract prepared from Example 2 was also orally administered to the experimental male mice using the same method as above.

In addition, as a control, the experimental male mice received only 1% hydroxypropylmethylcellulose used in suspension instead of the non-repair extract.

(3) Measurement of body weight of male laboratory mice in low dose group, high dose group and control group

In the low-dose group and the high-dose group, the weight of the experimental male mice was measured three times per week during the administration period of the non-repair extract, and the average body weight was obtained using the same. The results are shown in Table 1 .

Average body weight (g) of experimental male mice by sample sample Average body weight of male mice (g) 3 days ^a 5 days ^a 7 days ^a 10 days ^a 12 days ^a 14 days ^a 17th ^a Example 1
(Low dose group) 30.1 ± 1.8 ^b 29.0 ± 1.6 ^b 29.0 ± 1.5 ^b 29.6 ± 1.3 ^b 29.1 ± 1.5 ^b 30.1 ± 1.1 ^b 29.9 ± 1.3 ^b Example 1
(High dose group) 28.6 ± 1.2 ^b 27.9 ± 1.4 ^b 27.1 ± 2.2 ^b 27.6 ± 1.8 ^b 27.4 ± 1.2 ^b 28.3 ± 1.0 ^b 28.5 ± 1.3 ^b Example 2
(Low dose group) 28.4 ± 1.1 ^b 28.0 ± 1.0 ^b 27.9 ± 1.3 ^b 28.2 ± 1.6 ^b 27.6 ± 1.2 ^b 28.2 ± 1.5 ^b 28.2 ± 1.4 ^b Example 2
(High dose group) 29.0 ± 1.3 ^b 28.5 ± 1.2 ^b 28.4 ± 1.3 ^b 28.8 ± 1.1 ^b 28.5 ± 1.2 ^b 29.2 ± 1.4 ^b 29.9 ± 1.8 ^b 1% HPMC
(Control group) 29.0 ± 1.6 ^b 28.1 ± 1.4 ^b 27.7 ± 1.3 ^b 28.0 ± 1.3 ^b 27.7 ± 1.2 ^b 28.7 ± 1.4 ^b 29.3 ± 1.9 ^{b a} : after solid cancer cell transplantation, ^b : mean ± standard deviation

As shown in Table 1 , the average body weight of the male males in the low dose group and high dose group to which the non-repair extract was administered was kept constant. In addition, the average body weight of the male males in the low dose group, high dose group and the control group did not show a significant difference. Through this, it can be seen that the non-repair extract according to the present invention does not exhibit systemic toxic effects.

(4) Measurement of solid cancer cell volume of male males in the experimental group in the low dose group, the high dose group and the control group

In addition, the volume of the solid cancer cells was calculated by the following equation (Clin Cancer Res, 2001, 7 , 2573-2580). In addition, the volume of solid cancer cells was measured after 10 days.

The results are shown in Table 2 .

Volume of solid cancer cells in experimental male mice (mm ³ ) sample Volume of solid cancer cells in experimental male mice (mm ³ ) 10 days ^a 11th ^a 12 days ^a 13th ^a 14 days ^a 15 days ^a 16th ^a 17th ^a Example 1
(Low dose group) 313
± 103 1031
± 338 1243
± 341 1386
± 363 ＊ 1893
± 486 ＊ 2655
± 702 ＊ 2986
± 777 ＊ 3816
± 1070 ＊ Example 1
(High dose group) 241
± 69 617
± 128 959
± 183 1302
± 259 ＊ 1493
± 318 ＊ 1906
± 449 ＊ 2271
± 555 ＊ 2796
± 650 ＊ Example 2
(Low dose group) 385
± 229 738
± 434 1025
± 446 1366
± 569 ＊ 1663
± 638 ＊ 2163
± 853 ＊ 2744
± 943 ＊ 3226
± 1230 ＊ Example 2
(High dose group) 354
± 134 679
± 221 992
± 292 1300
± 569 ＊ 1627
± 638 ＊ 1844
± 446 ＊ 2278
± 629 ＊ 2601
± 719 ＊ 1% HPMC
(Control group) 787
± 211 ^b 1838
± 419 2426
± 637 3605
± 1001 4932
± 1019 6623
± 1545 7655
± 1715 8909
± 2369 ^a : after solid cancer cell transplantation, ^b : mean ± standard deviation, *: statistical significance compared to control group (p <0.05)

As shown in Table 2, in the low-dose group and the high-dose group of male mice that were orally administered the non-repair extract, the volume of solid cancer cells was significantly reduced compared to that of the control male mice. In addition, the volume reduction of solid cancer cells in the high dose group was significant. Through this, it can be seen that the non-repair extract according to the present invention has anticancer activity.

Experimental Example 2 Cytotoxicity of Non-Repairing Extracts against Cancer Cells

In Experimental Example 2, MTT analysis was performed on the passaged cancer cells to determine the cytotoxicity against cancer cells of the non-repair extract according to the present invention. MTT (3- [4,5-dimethylthiazol-2-yl] -2,5-diphenyl tetrazolium bromide) analysis showed that MTT was a purple formazan by oxido-reductase in the mitochondrial lining of living cells. It is a method of measuring the cytotoxicity of a substance by measuring the survival rate of cells using a reaction that forms (formazan).

(1) culturing cancer cells

In order to examine the cytotoxicity of cancer cells of the non-repair extract according to the present invention, four types of cancer cells were cultured and used in a medium.

MCF-7, a human breast cancer cell line, was obtained from Seoul National University Cell Line Bank and cultured in RPMI 1640 medium containing 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin (Gibco BRL, USA). Was used.

Human colon cancer cell-derived cell lines Caco-2 and B16 melanoma cells were obtained from Seoul National University Cell Line Bank and contained Dulbecco's Modified Eagles's Medium (DMEM) medium containing 10% FBS and 1% penicillin-streptomycin (Gibco BRL, USA). It was used to culture.

Human lung cancer cell-derived cell line A-549 was obtained from Seoul National University Cell Line Bank and cultured in Ham's F21K medium (Gibco BRL, USA) containing 10% FBS, 1.5 g / L sodium hydrogen carbonate and 1% penicillin-streptomycin. Was used.

Culture method of the cancer cell-derived cell line was performed in the same manner as follows.

Each cell line stored at −196 ° C. was thawed in a 37 ° C. bath, then placed in a 100 mm ² tissue culture Petri dish and incubated in 37 ° C. and 5% CO ₂ incubator. If the cancer cells proliferate more than 90% at the bottom of the Petri dish while changing medium for 2-3 times a week, they are washed with PBS (phosphate buffered solution) and treated with 0.05% trypsin-EDTA (Gibco BRL, USA) for 3 minutes. The cancer cells were passaged while the cancer cells were separated from the bottom of the Petri dish.

(2) MTT assay (cytotoxicity experiment)

The experimental method is as follows.

The passaged cancer cell lines were dispensed with about 2 × 10 ⁴ cells per well in a 96 well plate and incubated in 37 ° C. and 5% CO ₂ incubator. The experimental group was cultured for 6 hours, after which the cancer cells were attached to the bottom of the well, 500 μg / ml of the non-repair extract prepared in Example 1 was the highest concentration, and 500 μg / ml of the non-repair extract was treated by diluting the culture solution three times. The cancer cell lines were incubated for 48 hours at 37 ° C. and 5% CO ₂ incubator. MTT (Sigma, St. Louis, Mo. USA) was dissolved in PBS to prepare a 5 mg / ㎖ MTT solution and 50 μl of each prepared MTT solution was added and incubated for 4 hours.

To obtain purple formazan formed by the reaction of MTT and redox enzyme, 150 μl of DMSO (dimethylsulfoxide) was added to each well, and the absorbance was measured at 540 nm.

As a control, adriamycin (Adriamycin, Sigma, St. Louis, Mo. USA), which is used as an anticancer agent, was used, and MTT analysis was performed using B16 melanoma cells as cancer cell lines.

Cell viability was measured by the absorbance obtained through the MTT assay and the following equation (2).

The concentration (IC ₅₀ , μg / ml) of the non-aqueous extract when the cell viability obtained by the above equation was 50% was determined.

The results are shown in Table 3 .

IC ₅₀ against cancer cell lines (µg / ml) sample IC ₅₀ against cancer cell lines (µg / ml) MCF-7 Caco-2 A 549 B 16 Example 1 > 167 > 500 > 500 > 500 Adriamycin 0.8

As shown in Table 3 above, the non-repaired extract prepared from Example 1 according to the present invention showed a significantly higher IC ₅₀ compared to adriamycin. Therefore, it can be seen that the non-repair extract according to the present invention has very low or no toxicity to cancer cells. This indicates that the non-repair extract according to the present invention is not cytotoxic to normal cells.

Preparation Example 1 Oral Tablet Containing Non-Repairing Extract as Active Ingredient

100 g of the non-repair extract of Example 1 was mixed with 175.9 g of lactose, 180 g of potato starch and 32 g of colloidal silicic acid. 10% gelatin solution was added to the mixture, which was then ground and passed through a 14 mesh sieve. It was dried and the mixture obtained by adding 160 g of potato starch, 50 g of talc and 5 g of magnesium stearate was refined.

The components of the tablet are as follows.

Non-Repairing Extract of Example 1. . . . . . . . . . . . 100 g

Lactose 175.9 g

Potato starch ············· 180 g

Colloidal silicic acid. . . . . . . . . . . . . . . . 32 g

10% gelatin solution. . . . . . . . . . . . . . . . Quantitative

160g potato starch                     

Talc ・ ・ ・ ・ ・ 50g

Magnesium Stearate ... 5 g

Preparation Example 2 Preparation of Functional Foods Containing Non-Repairing Extracts as Active Ingredients

A functional beverage containing a non-repair extract was prepared by mixing 10 g of a non-repair extract of Example 1, 5 g of citric acid, 5 g of fragrance, a strong sweetener and 190 g of a pigment, and 4,240 g of purified water.

As described above, the non-repair extract according to the present invention has anti-cancer activity and can be usefully prepared by preparing a pharmaceutical composition, a dietary supplement, and a functional food for anticancer drugs, which have no cytotoxicity and contain it as an active ingredient.

Claims (8)

A pharmaceutical composition for anticancer drugs containing a non-repair extract as an active ingredient. The method of claim 1, wherein the non-repair extract 1) adding water to the non-repair, hot water extraction, and then filtering to prepare an extraction filtrate (step 1); And 2) The pharmaceutical composition for anticancer drugs, which is prepared through the step of concentrating the extract filtrate under reduced pressure and lyophilizing to prepare a non-repair extract (step 2). The method of claim 1, wherein the non-repair extract 1) adding alcohol to the non-repair, hot water extraction, and filtering to prepare an extraction filtrate (step 1); And 2) The pharmaceutical composition for anticancer drugs, which is prepared through the step of concentrating the extract filtrate under reduced pressure and lyophilizing to prepare a non-repair extract (step 2). 4. The pharmaceutical composition for anticancer drugs according to claim 3, wherein the alcohol is ethanol. A functional food having anticancer activity containing a non-repair extract as an active ingredient. The method of claim 5, wherein the non-repair extract 1) adding water to the non-repair, hot water extraction, and then filtering to prepare an extraction filtrate (step 1); And 2) Functional food, characterized in that the extraction filtrate is prepared through the step of concentrating under reduced pressure and freeze-drying to prepare a non-repair extract (step 2). The method of claim 5, wherein the non-repair extract 1) adding alcohol to the non-repair, hot water extraction, and filtering to prepare an extraction filtrate (step 1); And 2) Functional food, characterized in that the extraction filtrate is prepared through the step of concentrating under reduced pressure and freeze-drying to prepare a non-repair extract (step 2). The functional food according to claim 7, wherein the alcohol is ethanol.

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