KR100968712B1 - Magnetic Resonance compatible in vivo tissue window - Google Patents

Abstract

The present invention relates to a magnetic resonance imaging compatible in vivo observation device, and more particularly, it is possible to observe the internal cell changes under a microscope while the animal is alive, and MRI imaging in the state equipped with the in vivo observation device The present invention relates to an MRI compatible in vivo observation device.

Magnetic resonance imaging compatible in vivo observation device according to the present invention as described above comprises a top wing, a bottom wing, and the upper and lower connecting portion is located between the upper wing and the lower wing to accommodate the observation glass and the snap ring. Main body; And a snap ring detachably fixing the observation glass mounted on the bottom of the upper and lower connecting portions, wherein the main body and the snap ring are made of polyethylene (Poly Etilen).

Polyethylene, Snap Ring, In Vivo Observation System

Description

Magnetic Resonance compatible in vivo tissue window

The present invention relates to a magnetic resonance imaging compatible in vivo observation device, and more particularly, it is possible to microscopic observation in real time that the tumor growth process or the manufactured fluorescent substance is drug-targeted to the tumor in the state that the animal is alive, The present invention relates to a magnetic resonance imaging compatible in vivo observation device that enables magnetic resonance imaging (MRI) imaging even when the in vivo observation device is mounted.

To date, in animal experiments for making medical reagents, it has been difficult to observe in real time the growth of tumors or the production of fluorescent substances to target drugs in the living animals.

In order to overcome this difficulty, the applicant has previously filed in Patent Registration No. 10-0753928, filed with the glass to open the site to be observed so that the experimental animal can be observed in real time in a live state, the cell or tissue We have also proposed a 'real-time observation device for specific sites in laboratory animals and observation methods using the same' that can observe the growth process of the microscope and the microscopic observation in real time that the drug is targeted to the tumor when the fluorescent material is injected.

1 to 3 is an exemplary view showing a conventional observation device, the conventional observation device 1 is a main body 2, the observation glass (3), the snap ring (4) and the suture groove (5). In particular, the main body 2 is located between the upper wing 11, the lower wing 12 and the upper wing 11 and the lower wing 12 and the observation glass (3) and snapping It consists of an upper and lower connection part 13 which receives the ring 4.

The snap ring 4 is placed on the upper portion of the viewing glass 3 to detachably fix the viewing glass 3 mounted on the bottom of the upper and lower connecting portions 13.

In addition, the main body 2 and the snap ring 4 may be applied to a living laboratory animal, and a titanium (Ti) material which is less harmful to a living body is used.

However, real-time microscopic observation is possible when using such a titanium material, but when using the magnetic resonance imaging (MRI) device as a means to obtain additional information beyond the microscopic findings in the strong magnetic field As a result, the image quality may be reduced and a problem may occur in the safety of the device.

In order to solve the above problems, the present invention allows the tumor, brain, neovascularization or joints to be observed under various microscopes in addition to the fluorescence microscope while the animal is alive, and by using a polyethylene (Poley Etilen) material The purpose of the present invention is to provide a magnetic resonance imaging compatible in vivo observation device that can reduce the harm to the human body and also allow MRI (Magnetic Resonance Imaging) imaging.

In addition, the present invention provides a magnetic resonance imaging compatible in vivo observation device that improves the structure of the snap ring so that the snap ring can be inserted even by the force of a human hand as a method for mounting the snap ring in the body according to the use of polyethylene material There is another purpose.

Magnetic resonance imaging compatible in vivo observation device according to the present invention for achieving the above object is located between the upper wing, the lower wing, and the upper wing and the lower wing to accommodate the observation glass and snap ring. A main body including an upper and lower connecting parts; And a snap ring detachably fixing the observation glass mounted on the bottom of the upper and lower connecting portions, wherein the main body and the snap ring are made of polyethylene (Poly Etilen).

Preferably, the snap ring is a ring body having a 'C' shape; A first end portion integrally formed with the ring body; And a second end formed integrally with the ring body, the second end being spaced apart from the first end by a predetermined distance on the opposite side of the first end, wherein the human hand contacts the ends of the first and second ends. It characterized in that the protrusions of a certain height are formed so that the force can be applied to each.

More preferably, the protrusion has a rectangular cross section or a curved arc cross section.

More preferably, the first and second ends are characterized in that the through-holes to which a force is applied by inserting a forceps means are further formed.

On the other hand, preferably, the protrusion is a vertical portion which is formed at a predetermined height above each of the first and second ends; And a horizontal portion each coupled to the upper surface of the vertical portion to have a space spaced from each upper surface of the first and second ends.

Magnetic resonance imaging compatible in vivo observation device according to the present invention as described above can observe the tumor, brain, neovascularization or joints in the living state of the microscope under a microscope, less harm to the human body and MRI ( Magnetic Resonance Imaging)

In addition, the magnetic resonance imaging compatible in vivo observation device according to the present invention has the effect that the snap ring can be fitted even by the force of the human hand according to the use of polyethylene material.

Hereinafter, with reference to the accompanying drawings will be described in detail with respect to the MRI in vivo observation device according to a preferred embodiment of the present invention.

4 is a perspective view showing the structure of a snap ring of the in vivo observation apparatus according to the first embodiment of the present invention, Figure 5 is a perspective view showing the structure of a snap ring of the in vivo observation device according to a second embodiment of the present invention , And FIG. 6 is a perspective view illustrating a structure of a snap ring of an in vivo observation device according to a third exemplary embodiment of the present invention.

Magnetic resonance image conformity in vivo observation apparatus according to the present invention has the same configuration as the in vivo observation apparatus according to the prior art, in the following in the magnetic resonance image conformity in vivo observation apparatus according to the present invention with reference to FIGS. I will explain.

1 to 3, magnetic resonance imaging compatible in vivo observation device 1 according to the present invention, the body 2, the observation glass (3), the snap ring (4) and the suture groove (5) It is configured to include.

The main body 2 is the lower wing portion 12 is in direct contact with the skin surrounding the observation target area of the experimental animal and the material of the main body 2 is preferably made of polyethylene (Pole Etilen) without magnetic force. At this time, the observation site corresponds to all sites for making medical reagents such as tumors, brain, neovascularization, and joints.

The cross-sectional shape of the main body 2 is not limited in the shape of a circle, a square, etc., but it is more preferable to have a circular shape so as to provide the convenience of suture and observation while minimizing the area touching the skin.

The main body 2 is composed of the upper wing portion 11, the lower wing portion 12 and the upper and lower connecting portion 13, the shape is preferably made of a hollow polygon.

The size of the main body 2 is variable according to the size of the experimental animal to be applied, in the case of the mouse, the outer diameter of the upper end portion is approximately 21 ￠, the outer diameter of the lower wing 12 may be approximately 17 ￠. At this time, the connecting portion connecting the upper and lower ends is more preferably manufactured with an outer diameter of about 16 mm smaller than the lower wing 12.

However, the size of the outer diameter is not limited thereto, and may be appropriately adjusted according to the overall size of the experimental animal.

The wing diameter of the upper wing 11 and the lower wing 12 is preferably about 2.3 mm in the case of a mouse, and may be appropriately adjusted according to the overall size of the experimental animal.

At the end of the upper wing portion 11 of the main body 2 is provided with one or more suture grooves 5 to be maintained in a state in which the skin is cut along its circumference.

The number of the suture grooves 5 is more preferably formed in the range of 4 to 8 depending on the cross-sectional shape of the main body (2).

At this time, the glass 3 according to the present invention preferably uses the cover glass 3 for the microscope in consideration of visual observation as well as the case when the microscope is required.

The size of the glass 3 is sufficient to observe the inside with a naked eye or a microscope using a 12 ￠ microscope glass when applied to a mouse, it is also possible to adjust according to the overall size of the experimental animal.

In addition, by mounting the snap ring 4 on the upper portion of the glass 3, the glass 3 can be detachably fixed. The snap ring 4 according to the present invention has a C shape and the material is poly It is made of ethylene (Pole Etilen).

The size of the snap ring (4) is sufficient if the outer diameter is approximately 14 ￠ when applied to the mouse can be appropriately adjusted according to the total size of the experimental animal.

Referring to FIG. 4, the snap ring 20 of the in-vitro observation device according to the first embodiment of the present invention is formed with a ring body 21 having a 'C' shape and integrally formed with the ring body 21. It consists of a first end portion 22 and the second end portion 23 which is formed integrally with the ring body 21 to be spaced apart from the first end portion 22 by a predetermined interval.

The first end 22 and the second end 23 are spaced apart by a predetermined interval, and have a separated structure. And, the snap ring 20 is made of a polyethylene material can be elastically bent by the force applied from the outside.

Protruding portions 24 are formed at the ends of the first and second ends 22 and 23, to allow the human hand to contact and apply a force to bend the entire snap ring 20, and the height of the protruding portions 24 is It is desirable to have a size similar to the thickness of a human finger.

On the other hand, the protrusion 24 is preferably formed integrally with the same material as the first and second ends 22, 23, in the first embodiment it is preferable that the protrusion 24 has a rectangular cross section.

Referring to FIG. 5, the snap ring 30 of the in vivo observation device according to the second embodiment of the present invention is similar to the structure of the snap ring 20 according to the first embodiment, except that the protrusion 31 is curved. There is a difference in that it is formed to have an arc-shaped cross section. This is a shape that can be easily applied to the finger to the curved arc (31a) portion when applying the force by holding the hand.

On the other hand, the snap ring (20, 30) of the in vivo observation device according to the first and second embodiments of the present invention can be applied to the force by inserting a force means (not shown) in the first, second ends (22, 23) Each through hole 25 may be further formed. The size of the through hole 25 is sufficient if the forceps means (not shown) can be inserted.

Referring to FIG. 6, the protrusion 43 of the snap ring 40 according to the third exemplary embodiment of the present invention has a vertical portion 43a formed at a predetermined height to each of the first and second ends 41 and 42. And a horizontal portion 43b each coupled to the upper surface of the vertical portion 43a so as to have a space therebetween from the upper surfaces of the first and second ends 41 and 42.

The first end portion 41 and the second end portion 42 are spaced apart by a predetermined interval and have a separated structure. And, the snap ring 40 is made of a polyethylene material can be elastically bent by the force applied from the outside.

7 is a flowchart illustrating a process of suturing a magnetic resonance imaging compatible in vivo observation device according to an embodiment of the present invention to a specific site in the experimental animal.

Referring to FIG. 7, first, an experimenter anesthetizes an experimental animal (S401), and then a lower end of a main body 2 of a magnetic resonance image compatible in vivo observation device 1 for observing a specific site in an experimental animal at a specific site to be observed. The wing 12 is mounted (S403).

After the step (S403), the upper and lower connecting portions 13 of the main body 2 are provided with the observation glass 3 and the snap ring 4 in sequence (S405).

The specific site corresponds to all sites used in animal experiments to make medical reagents such as tumors, brain, neovascularization, and joints.

After the step S405, the suture groove 5 formed in the upper wing portion 11 on the connection portion is fixed using a surgical thread to fix the upper wing portion 11 to a specific portion to be observed ( S407).

After the suture process as described above it can be observed through the glass (3) part of the cell or tumor growth process or the manufactured fluorescent material targeted to the tumor or cells in real time through the naked eye or microscope.

In addition, it is possible to miniaturize the size of the magnetic resonance imaging compatible in vivo observation device (1) including the body (2), glass (3) and snap ring (4) in addition to the tumor, cells, can be applied to the brain or joint.

Experimental Example

The main body 2 of the magnetic resonance imaging compatible in vivo observation device 1 of the present invention is the upper wing portion 11 and the imaging device (1) of the imaging device (1) to be placed in order to the glass 3 and the snap ring (4) It consists of a connecting portion for connecting the lower wing portion 12 and the upper and lower ends directly in contact with the skin of 1), wherein the outer diameter of the upper wing portion 11 is wider than the outer diameter of the lower wing portion 12, the lower wing portion ( The outer diameter of 12) was made to have a wider cylindrical shape than the connecting portion, and the material was made of polyethylene (Poley Etilen) material.

In this case, the main body 2 had a size of 21 mm and a wing width of 2.3 mm at the upper wing portion 11, an outer diameter of the lower wing 12 at 17 mm, and a blade width of 2.3 mm. It was made to be 16 ￠. Around the upper wing 11, eight suture grooves 5 were drilled at 45 degree intervals using a drill.

In addition, the glass (3) used a 12 ￠ microscope cover glass, the snap ring (4) was to have a C shape and the material was used polyethylene (Poly Etilen).

BALB / C nude mouse (male, 25g, 6W) was used as the experimental animal for observation. After injection of epithelial cancer cell KB cell, it was grown for 2 weeks. ) Shooting was performed.

8 and 9 show images when taken under a microscope, and FIGS. 10 and 11 show images taken when taking a magnetic resonance imaging (MRI).

Although the preferred embodiment of the present invention has been described above, it is clear that the present invention may use various changes, modifications, and equivalents, and may be applied in the same manner by appropriately modifying the above embodiments. Accordingly, the above description does not limit the scope of the invention as defined by the limitations of the following claims.

1 to 3 is an exemplary view showing a conventional observation device;

4 is a perspective view showing the structure of a snap ring of the in vivo observation device according to a first embodiment of the present invention;

5 is a perspective view showing the structure of a snap ring of the in vivo observation apparatus according to a second embodiment of the present invention;

6 is a perspective view showing the structure of a snap ring of the in vivo observation apparatus according to a third embodiment of the present invention;

7 is a flowchart illustrating a process of suturing a magnetic resonance imaging compatible in vivo observation device according to an embodiment of the present invention to a specific site in the experimental animal;

8 and 9 are images shown during microscopy; And

10 and 11 are images when the magnetic resonance imaging (MRI) photographing.

※ Explanation of symbols for main parts of drawing

1: in vivo observation device 2: main body

3: observation glass 4, 20, 30, 40: snap ring

5: suture groove 11: upper wing

12: lower wing portion 13: upper and lower connecting portions

21: ring body 22, 41: first end

23, 42: second end 24, 31: protrusion

25: through hole

Claims (5)

A main body including an upper wing part, a lower wing part, and an upper and lower connection part positioned between the upper wing part and the lower wing part to receive the observation glass and the snap ring; And a snap ring detachably fixing the observation glass placed on the bottom of the upper and lower connecting portions. The body and the snap ring are magnetic resonance imaging compatible in vivo observation device, characterized in that made of polyethylene (Poly Etilen) material. The method of claim 1, The snap ring has a ring body having a 'C' shape; A first end portion integrally formed with the ring body; And a second end formed integrally with the ring body and spaced apart from the first end by a predetermined distance on the opposite side of the first end. Magnetic resonance imaging suitability in vivo observation device, characterized in that the protrusions of a certain height are formed at each end of the first and second ends so that human hands can contact and apply a force. 3. The method of claim 2, Magnetic resonance imaging compatible in vivo observation device, characterized in that the protrusion has a rectangular cross section or a curved arc cross section. The method of claim 2 or 3, Magnetic resonance imaging suitable in vivo observation device, characterized in that each of the first and second ends is further formed with a through hole for applying a force by inserting the forceps means. 3. The method of claim 2, The protruding portion is a vertical portion formed at a predetermined height to each upper portion of the first and second ends; And a horizontal portion each coupled to an upper surface of the vertical portion so as to have a space spaced from the upper surfaces of the first and second ends, respectively.

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