KR100940511B1 - A Metabolic Diagnosis System and Method based Mitochondria - Google Patents

Abstract

The present invention has a consistent system of physiological networks of diseases ranging from genes to organ systems, and mitochondrial-based metabolism enables comprehensive implementation of diagnosis, prescription, and prevention by using a systematic approach through computer simulators and databases. The present invention relates to a diagnostic system and a method of diagnosis.

The mitochondrial based photodecision system of the present invention includes a mechanism information database 110 including a disease data 111 and a disease prognosis data 112, a genomic data 121, and a personal medical history data 122. A database server 100 including a genetic information database 120 and a personal information database 130 including a physiological function data 131 and a clinical diagnosis result 132; Clinical test means (210) comprising a mitochondrial heat production diagnostic kit (211) for evaluating the heat production capacity of the mitochondria and a cytoplasmic hybrid kit (212) for examining and evaluating the density and type of mitochondrial DNA of the patient And a clinical diagnosis database 220 storing information related to the clinical diagnosis , and clinical diagnosis means 230 matching the information examined by the clinical examination means with the information of the clinical diagnosis database and calculating a clinical diagnosis result. A mitochondrial system module 200; And an energy system simulator (300) connected to the mitochondrial system module to receive test and diagnostic information, and to perform a simulation and analysis based on the test and diagnostic information and information stored in the database server. ; Characterized in that comprises a.

On the other hand, as another aspect according to the present invention based on the mitochondria, the method, the mechanism information database 110, the genetic information database 120 and the personal information database in which the individual medical history / genetic information / energy metabolic information is stored Accessing the database server 100 including 130 and extracting necessary information; Cardiovascular and metabolic information of the individual is measured and obtained by the mitochondrial system module 200 based on the extracted medical history / genetic information / energy metabolism information; Collecting the information in the two steps and calculating a diagnosis result by the energy system simulator (300); And determining the metabolic syndrome prognosis based on the calculated diagnosis result. Characterized in that it comprises a.

Mitochondria, Grand Prize, Obesity, Diabetes

Description

A metabolic diagnosis system and method based Mitochondria

1 is a comparison of the increase in volume and surface area at scale increase.

Figure 2 is a conceptual diagram of a derivation approach of the human body heat balance body temperature scale law by bioenergy.

Figure 3 is a conceptual diagram for explaining the scale law heat generation according to the body weight widely recognized in biology.

Figure 4 is a mitochondria-based photographic system according to the present invention.

** Description of the symbols for the main parts of the drawings **

1000: Mitochondria-based Photo Deputy System

100: database server

110: Mechanism Information Database

111: Disease data 112: Disease prognosis data

120: Genetic Database

121: genomic data 122: personal medical history data

130: personal information database

131: physiological function data 132: clinical diagnostic data

200: mitochondrial system module

210: clinical test means

211: Mitochondrial heat production diagnostic kit

212: Cytoplasmic Hybrid Diagnostic Kit

220: clinical examination database

230: Clinical diagnostic means

300: energy system simulator

The present invention relates to a mitochondrial-based estimator system, and more particularly, to a mitochondrial-based estimator system and a diagnosing method for diagnosing diseases related to obesity by investigating the characteristics of the mitochondria in connection with the human heat and circulation system. .

Mitochondria are basically organs that animals have to maintain life. Hundreds to thousands are present in the cells, and they are organelles involved in cellular respiration. In other words, the more breathable cells contain a large amount of mitochondria, and mitochondria play a pivotal role in bioenergy metabolism. The mitochondria are unique among animal organelles of animals, and in response to the fact that mitochondria have independent DNA, mitochondria and aerobic respiration as primitive life emerge, The most widely accepted hypothesis is that nuclear cells maintain the symbiotic relationship with each other and then undergo the endosymbiosis process, which is integrated into one cell. Through this symbiotic process, mitochondria are thought to have transmitted most of their genetic information to the nucleus. Currently, most proteins that make up the mitochondria are encoded from the nucleus, but important proteins related to ATP production are encoded only by their own DNA. Mitochondrial DNA is in an environment in constant contact with oxygen and free radicals, but it is not easily protected by histone proteins as compared to nuclear DNA, and genetic mutations can easily occur because of its inability to repair damage. In fact, it is well known that mitochondrial DNA occurs more than 10 times faster than nuclear DNA, and most mitochondrial DNA codes for proteins, so if mutations occur, they are more likely to go wrong. Mitochondrial dysfunction has been found to be deeply related to cancer, cerebral neuropathy, and aging, and thus mitochondrial-related research is rapidly progressing worldwide.

Meanwhile, metabolic syndrome (Metabolic Syndrome), which is a symptom of various adult diseases such as diabetes, obesity, hypertension, stroke, heart disease, etc. in one person, is present in 30-40% of the adult population. Has recently been found to have a strong tendency to cause mitochondrial dysfunction. Regarding the correlation between mitochondrial dysfunction and the onset of diabetes, a 1993 survey of residents in Yeoncheon, Gyeonggi-do, observed a decrease in mitochondrial DNA before diabetes. This is a preceding phenomenon, not a backward action by diabetes, suggesting that the body's energy generation mechanism is closely related to obesity and diabetes. Briefly, this relationship is as follows. First, as mitochondrial DNA decreases, the amount of energy produced by mitochondria also decreases, and energy metabolism occurs due to temperature homeostasis with respect to temperature. In other words, energy metabolism occurs to replenish the amount of heat (energy) depleted by the reduction of mitochondria to maintain body temperature. Therefore, the volume of adipose tissue increases, and as a result, the weight also increases. Increasing human weight and increasing triglycerides increases insulin resistance and ultimately leads to a combination of symptoms such as diabetes and high blood pressure.

The mitochondrial hypothesis is associated with the following four laws of physics: the law of conservation of energy in the human body, the law of scaling on basal metabolism, the constant temperature / body mass conservation law of body temperature, and the correlation between body weight change and heat dissipation rate. Regarding these laws of physics, the mitochondrial hypothesis is explained in more detail as follows. Given that weight and volume are proportional, there is an exponential difference between the increase in volume and the increase in surface area. Referring to FIG. 1, as the length is increased by 2 times, the surface area is increased by 2 ² times or 4 times, and the volume is increased by 2 ³ times or 8 times. On the other hand, when considering the principle of heat transfer, it is well known that the larger the surface area, the more actively the heat transfer occurs, and the surface area and the heat transfer amount are in proportion. Taken together, the increase in surface area with increasing volume is much smaller compared to the increase in volume, and thus the rate of energy consumption (directly related to the increase in surface area) and the rate of energy generation (directly related to volume increase). ), The ratio value is reduced. The larger the creatures living in the colder regions, the larger their size is due to the evolutionary effect of losing their body temperature to the environment according to this principle. However, when the amount of heat generated in the human body is reduced due to the impairment of the function of the mitochondria, the energy balance is collapsed and the body temperature is increased. Accordingly, in order to maintain body temperature, the energy consumption rate must be reduced, and when the volume of adipose tissue increases due to the compensating action and the body volume increases, the ratio of energy consumption rate / energy generation rate can be reduced to maintain body temperature. However, this increase in weight (and volume) is not only able to maintain body temperature, but also obesity, and as a result, the possibility of developing metabolic syndrome, which is a combination of symptoms such as diabetes, hypertension, accompanied by obesity becomes very high.

However, conventionally, symptoms such as diabetes, hypertension, obesity, and the like were diagnosed based on the following factors such as blood pressure and blood sugar, that is, there was a shortage of the provision of treatment measures only after the symptoms progressed to some extent. On the other hand, in order to complement the conventional diagnostic method, research and efforts on the diagnostic method that can detect the signs of obesity and metabolic syndrome in advance and appropriately have been in progress unlike the conventional diagnostic methods.

Accordingly, the present invention has been made to solve the problems of the prior art as described above, and an object of the present invention is to provide a system through a computer simulator and a database with a consistent system for the physiological network of diseases ranging from genes to organ systems. The present invention provides a mitochondrial-based escort system and its essay method that enable comprehensive implementation of diagnosis, prescription, and prevention by using a general approach.

The mitochondrial based photodecision system of the present invention for achieving the object as described above, the mechanism information database 110, the genome data 121 and the personal medical history comprising the disease data 111 and the disease prognosis data 112 A database server 100 including a genetic information database 120 including data 122 and a personal information database 130 including physiological function data 131 and clinical diagnosis results 132; Clinical test means comprising a mitochondrial heat production diagnostic kit 211 for evaluating the heat production capacity of the mitochondria and a cytoplasmic diagnostic kit 212 for investigating and evaluating the density and type of mitochondrial DNA of the patient (210) ), The clinical diagnosis database 220 storing information related to the clinical diagnosis , and the clinical diagnosis means 230 matching the information examined by the clinical examination means with the information of the clinical diagnosis database and calculating the clinical diagnosis result. A mitochondrial system module (200) formed by; And an energy system simulator (300) connected to the mitochondrial system module to receive test and diagnostic information, and to perform a simulation and analysis based on the test and diagnostic information and information stored in the database server. ; Characterized in that comprises a.

At this time, the weight gain rate of the human body is characterized by simulating and calculating using a modified human body mass conservation equation, such as the following equation.

Here, M: weight, EI: energy intake, PAL (Physical Activity Level): physical activity indicator, BMR (Basal Metabolic Rate): basic metabolic rate, c: conversion constant) In this case, the environmental impact variable is Characterized in the equation.

(Where a: environmental impact coefficient, M _TE : thermal equilibrium weight)

Where a is obtained from a database created by analyzing clinical data, and M _TE is obtained from the principle of temperature stability. The rate of change in body temperature is determined by the difference between the amount of heat produced and the amount of heat dissipated and is formulated as follows.

The Q _dissipation shown above remains almost constant, whereas Q _production varies depending on the function and metabolic rate of mitochondria, and is expressed as follows according to scale law. That is, human heat production is estimated by considering mitochondrial heat production functional factors based on the basic metabolic rate which has a correlation with weight.

In addition, the mitochondrial heat production diagnostic kit 211 is characterized by measuring the mitochondrial properties including the mitochondrial index k _d value according to the mitochondrial dysfunction ratio k _m value and mitochondria type. At this time, the mitochondrial heat production diagnostic kit 211 preferably comprises a nanobio device, an optical device and a non-contact temperature sensor.

In addition, the cytoplasmic hybrid diagnostic kit 212 is characterized in that to obtain the cytoplasmic hybrid of the patient using a platelet of the patient.

On the other hand, as another aspect in accordance with the present invention based on the mitochondria photoshooting method,

(a) necessary information connected to a database server 100 including a mechanism information database 110, a genetic information database 120, and a personal information database 130 in which individual medical history / genetic information / energy metabolic information is stored; Is extracted;

(b) obtaining cardiovascular and metabolic information of the individual based on the extracted medical history / genetic information / energy metabolism information by measuring by the mitochondrial system module 200;

(c) collecting the information in steps (a) and (b) and calculating a diagnosis result by the energy system simulator 300; And

(d) determining the metabolic syndrome prognosis based on the calculated diagnosis result; Characterized in that it comprises a.

Hereinafter, the mitochondrial-based photographic system according to the present invention having the configuration as described above will be described in detail with reference to the accompanying drawings.

FIG. 2 conceptually illustrates a derivation approach of the thermobalance body temperature maintaining scale law by bioenergy. First, the macroscopic conservation law of the human thermal system is derived by the phenomenological system analysis method. However, as described above, it is known that the function of the mitochondria greatly affects the operation of the human body's thermal system. Therefore, it can be seen that the macroscopic conservation law of the human body system has an effect related to the mitochondrial function. Therefore, a system model of individual thermal systems is obtained as ordinary differential equations using the mitochondrial heat production indicators, the human body's heat generation and dissipation clinical metabolic indicators, and the law of energy conservation and temperature constant.

On the other hand, by accessing the microbiological molecular biology, the mitochondria itself can obtain various characteristics such as mitochondrial signaling system, genomic information, and protein information. Can be. This information can be used to evaluate the heat production capacity of the unit mitochondria.

As shown in FIG. 2 and described above, it can be seen that the unit mitochondrial function has a significant effect on the individual thermal system. Therefore, the mitochondrial-based estimator system according to the present invention derives the scale law of human body heat balance temperature using the system model and the information on the unit mitochondrial function in the individual thermal system, thereby multi-dimensional from the mitochondrial gene to the human system. It also provides a systematic approach.

As for the individual thermal system, it is well known in the biological system that the law of conservation of mass of the human body can be expressed by the following equation.

In Equation 1, M is weight, EI is energy intake, PAL (Physical Activity Level) is physical activity indicator, BMR (Basal Metabolic Rate) is the basic metabolic amount, c is the conversion constant. However, as described above, when the temperature of the external environment of the human body is lowered or damage to the function of the mitochondria occurs, the amount of heat dissipated to the external environment through the human body surface is greater than the amount of heat generated by the mitochondria. The effect of achieving thermal equilibrium by increasing the size of the body and increasing its size cannot be explained by Equation (1). In particular, since this phenomenon is a phenomenon that occurs over a long time and the effect appears in the past, this part was previously considered to be a negligible error, but this is a fact that a recent approach has been found to be a wrong approach.

In the present invention, to propose a new human body mass conservation equation modified by the effect. That is, by adding the environmental influence variable as shown in Equation 2 below, it is possible to theoretically calculate the influence related to cold environment or damage to mitochondrial function.

On the other hand, the energy stored in the human body system can be calculated by modeling as shown in Equation 3 by the energy conservation law.

Each term is, in order, the rate of change of energy stored in the human body system, the rate of change of heat flowing into the human body system, the rate of change of heat flowing out of the human body system, the rate of change of heat generated in the system, and the rate of change of daily energy entering the body system. To simplify the above equation, it is the same as Equation 4 below, and as described in Equation 4, the rate of change of energy stored in the human body system is approaching zero because the human body system tries to achieve thermal equilibrium. .

That is, the temporal change rate of energy stored in the human body system can be obtained by subtracting the thermal change rate dissipated in the human body system from the thermal change rate generated in the human body system. At this time, the heat generation amount and heat dissipation amount in the human body system can be obtained as shown in Equation 5 below.

In the heat generation equation, k _m is the ratio of dysfunction of the mitochondria and k _d is the mitochondrial index according to the mitochondrial type. As the rate of dysfunction of mitochondria increases, the amount of heat generated decreases, while the amount of heat dissipation remains almost unchanged. Therefore, the heat dissipation amount is greater than the heat generation amount in Equation 4, so that the body temperature tends to decrease, and the physiological mechanism for defending this is activated. If such a functional disorder occurs very rapidly, the human body exerts a short-term control function (acted by the action of the sympathetic nerve, the body swinging behavior, creepy rise, etc.) to increase the amount of heat generated in order to resist it. Maintain body temperature at all times. However, this only lasts for a short period of time, and if dysfunction persists, the heat-compensation function achieved by short-term control is gradually replaced by long-term weight gain. This is shown in FIG. 3. Here, the amount of heat generated by body weight follows the scale law widely accepted in biology. In other words, the basic metabolism of all living things is log-scale proportional to the weight. By the way, the basic metabolic amount of the human body is eventually dissipated as heat, which is consistent with the heat dissipation amount, and based on this, the line of scaling law of the weight-to-heat generation amount is shown in FIG. 3. In addition, if the amount of heat dissipation is assumed here, it may be displayed as a horizontal line. In other words, the point where the scale law of heat generation amount and the horizontal line of heat dissipation amount is the initial thermal equilibrium weight. However, if the amount of heat generated decreases for some reason, the scale law line moves downward, thus creating a gap between the amount of heat generated and the amount of heat dissipated. However, in order to maintain the temperature at all times, additional heat is generated by short-term self-regulation function, thereby achieving a balance between heat generation and heat dissipation. However, if the decrease in heat generation continues, long-term adaptation to gradually increase the weight instead of the short-term compensation function occurs, it occurs along the arrow shown in ② of FIG. This long-term adaptation lasts until the heat generation increase due to weight gain completely offsets the heat dissipation and achieves thermal equilibrium. This new thermal equilibrium weight is defined as the thermal equilibrium weight and is expressed as M _TE . The weight gain factor occurs as much as the difference between the current weight M and the thermal equilibrium weight M _TE (MM _TE ). This is based on the theory of the feedback control system. In summary, the equation for the environmental influence variable as in Equation 2 is derived.

The heat generation amount in Equation 5 is also operated as an environmental influence variable in Equation 2. Therefore, the following Equation 6 can be calculated by combining Equations 2 and 5.

In the above formula, M _TE is the weight at thermal equilibrium, and a is the conversion constant. That is, as can be seen from Equation 6, the modified human body mass conservation equation can be obtained from the conventional human body mass conservation equation by grasping the characteristics of the mitochondria. According to the mitochondria-based photodetector system according to the present invention, the weight gain rate of the human body can be calculated more accurately than before by using the modified body mass conservation equation.

4 is a conceptually brief illustration of a mitochondrial based photodecision system according to the present invention. As shown, the photograph system 1000 according to the present invention includes a database server 100, a mitochondrial system module 200, and an energy system simulator 300.

The database server 100 may include a mechanism information database 110 including a disease data 111 and a disease prognosis data 112, a genetic information database including a genomic data 121 and a personal medical history data 122. 120) and a personal information database 130 including physiological function data 131 and clinical diagnosis results 132. In this case, the personal medical history data 122 refers to a personal medical history as a general case, such as how the personal medical history is developed, not the personal medical history as personal information, and the information included in the personal information database 130. Is distinct information.)

The mitochondrial system module 200 includes a clinical test means 210 for collecting and evaluating a mitochondria of a patient, a clinical diagnosis database 220 storing information related to clinical diagnosis, and information examined by the clinical test means and the clinical test. Clinical diagnostic means 230 is included to match the information in the diagnostic database and to produce clinical diagnostic results. The clinical test means 210 comprises a mitochondrial heat production diagnostic kit 211 and a cytoplasmic hybrid (cybrid) diagnostic kit 212 as shown, wherein the mitochondrial heat production diagnostic kit 211 is nano It comprises a bio-device, an optical device and a non-contact temperature sensor to evaluate the heat production capacity of the mitochondria, the cytoplasmic hybrid diagnostic kit 212 investigates and evaluates the density and type of mitochondrial DNA of the patient. In particular, in the present invention, the cytoplasmic hybrid diagnostic kit 212 performs diagnosis immediately using platelets of the patient, unlike the conventional fibroblast cells used to culture the cytoplasmic hybrid. Platelets are only cytoplasm away from the megakaryocytes of the bone marrow, eliminating the process of removing the nucleus, and also obtaining a large amount from peripheral blood, which is a relatively non-invasive method and does not need to be cultured. In addition, there is a very convenient advantage in storage and transport, such as can be used by thawing after freezing. The mitochondrial system module 200 can obtain the mitochondrial index k _d according to the mitochondrial dysfunction ratio k _m and the mitochondrial type used in Equation 6.

The energy system simulator 300 is connected to the mitochondrial system module to receive test and diagnostic information, and to perform a simulation and analysis based on the test and diagnostic information and information stored in the database server to calculate a diagnosis result. do. The diagnosis result has a form of a personal energy metabolism DB specifically, and shows the energy metabolism information of the human body.

The energy system simulator 300 calculates the weight increase rate of the human body system of the patient according to Equation 6, and accordingly, the energy system simulator 300 ultimately maintains the state as it is using the weight increase rate. The progression of obesity and the development of metabolic syndrome associated with obesity is measured with much higher confidence than before.

As an embodiment of the present invention will be described below with respect to the photographic method that an individual can obtain and utilize the analysis results using the photographic system of the present invention. An individual A accesses an existing database server 100 containing his / her medical history / genetic information / energy metabolism information and extracts necessary information, and based on the extracted information, a mitochondrial system module (200) To measure cardiovascular and metabolic information. The data obtained from the above two processes are collected and input to the software calculation routine of the energy system simulator 300 used in the present invention. After performing the calculation, the result is stored in the energy metabolism DB of A again. It is possible to determine the metabolic syndrome prognosis by combining the stored calculation results and diagnostic data.

The present invention is not limited to the above-described embodiments, and the scope of application of the present invention is not limited to those of ordinary skill in the art to which the present invention pertains without departing from the gist of the present invention as claimed in the claims. Of course, various modifications can be made.

As described above, the present invention, according to the mitochondrial hypothesis, the mitochondrial functional impairment occurs in advance, the study results that obesity symptoms appear if this condition persists for a long time, by diagnosing the functional impairment of mitochondria in advance with obesity and obesity There is a great effect in predicting the possibility of metabolic syndrome and preventing it early.

In particular, according to the present invention, there is an effect to more effectively detect the functional damage of the mitochondria, and also by using a modified body mass conservation equation, it is possible to obtain more reliable diagnostic data.

In addition, according to the present invention, not only the effect of obesity and metabolic syndrome accompanying obesity can be diagnosed early, but also the cause of various diseases can be identified under the overall structure from the organelles to organs. Unlike the conventional short-term and local treatment and prescription for the symptoms of the has the effect of developing a long-term and overall treatment and prescription. In addition, the side effects of treatment are minimized, as well as the greater effect that can be much more comprehensive and effective to improve the health of the patient.

Claims (14)

A database server 100 including a mechanism information database 110, a genetic information database 120, and a personal information database 130; A mitochondrial system module 200 for examining and diagnosing clinical characteristics of the mitochondria ; And An energy system simulator (300) connected to the mitochondrial system module to receive test and diagnostic information, and to perform simulation and analysis based on the test and diagnostic information and information stored in the database server to calculate a diagnosis result; Including but not limited to, The diagnostic result is the body weight increase rate or body temperature change rate of the human body, The energy system simulator 300 simulates and calculates the weight gain rate of the human body using a modified human body mass conservation equation, such as

(M: weight, EI: energy intake, PAL (physical activity level): physical activity indicator, BMR (Basal Metabolic Rate): basal metabolic rate, c: conversion constant) Or the energy system simulator 300 is a mitochondrial based estimator system, characterized in that the body temperature change rate is calculated by the law of conservation of energy in the human body system such as the following equation.

(M: body weight, C _p : static specific heat, Q _inflow : heat change rate flowing into the human body system, Q _outflow : heat change rate flowing out of the human body system, Q _production : heat change rate generated in the system, dW / dt: human body Rate of change of work energy entering the system) delete The method of claim 1, The mitochondrial based estimator system is based on the mitochondrial based estimator system, which is based on the energy conservation law of the human body, the scaling law for the basic metabolic rate, the constant temperature / body mass preservation law of body temperature, and the correlation between body weight change and burn rate. . The method of claim 1, The mechanism information database (110) is a mitochondrial based escalator system characterized in that it comprises disease data (111) and disease prognosis data (112). The method of claim 1, The genetic information database (120) is a mitochondrial based escalator system characterized in that it comprises genomic data (121) and personal medical history data (122). The method of claim 1, The personal information database 130 is a mitochondrial based escalator system, characterized in that it comprises a physiological function data (131) and clinical diagnostic results (132). The method of claim 1, The mitochondrial system module 200 includes a mitochondrial heat production diagnostic kit 211 for evaluating the heat production capacity of the mitochondria and a cytoplasmic hybrid kit 212 for examining and evaluating the density and type of mitochondrial DNA of the patient. and made clinical test means (210), clinical that matches the information of the information and the clinical diagnostic database inspection by the clinical diagnostic database 220 and the clinical inspection means the information related to the clinical diagnosis is stored, and calculating the clinical diagnosis Mitochondrial based photodecision system, characterized in that comprises a diagnostic means (230). delete The method of claim 1, wherein the environmental impact variable Mitochondrial-based escalator system, characterized in that determined by the following equation.

(M _TE : thermal equilibrium weight, a: constant determined from physiological data) delete The method of claim 7, wherein the mitochondrial heat production diagnostic kit 211, A mitochondrial-based photographic system, characterized in that for measuring the mitochondrial properties including the mitochondrial dysfunction ratio k _m value and mitochondrial index k _d value according to the mitochondrial type. The method of claim 7, wherein the mitochondrial heat production diagnostic kit 211 Mitochondria-based photographic system, characterized in that comprises a nano-bio device, an optical device and a non-contact temperature sensor. The method of claim 7, wherein the cytoplasmic hybrid diagnostic kit 212 is A mitochondrial-based photographic system, characterized by obtaining cytoplasmic hybridization of a patient using platelets of the patient. In the mitochondria-based photographic method using the mitochondrial-based photographic system according to claim 1, The photographic method, (a) necessary information connected to a database server 100 including a mechanism information database 110, a genetic information database 120, and a personal information database 130 in which individual medical history / genetic information / energy metabolic information is stored; Is extracted; (b) obtaining cardiovascular and metabolic information of the individual based on the extracted medical history / genetic information / energy metabolism information by measuring by the mitochondrial system module 200; (c) collecting the information in steps (a) and (b) and calculating a diagnosis result by the energy system simulator 300; And (d) determining the metabolic syndrome prognosis based on the calculated diagnosis result; Mitochondrial-based photographic method comprising a.

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