KR100930809B1 - A pharmaceutical composition for prevention and treatment of gastritis, gastric ulcer and duodenal ulcer comprising red currant powder potato juice or starch as an active ingredient - Google Patents

Abstract

The present invention relates to a pharmaceutical composition for the prevention and treatment of gastritis, gastric ulcer and duodenal ulcer containing fresh juice or starch of Solanum tuberosum L. (cv. Redking valley), which is a new variety of potato, as an active ingredient.

The fresh juice or starch of Red King Valley potato according to the present invention shows a significant inhibitory action in gastric, gastric, and duodenal ulcer animal models, and thus can be effectively used as a preventive and therapeutic agent for gastritis, gastric ulcer, duodenal disease.

Red King Valley Potato, gastritis, gastric ulcer, duodenal ulcer, juice, starch

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a pharmaceutical composition for preventing and treating gastritis, gastric ulcer and duodenal ulcer including red juice or starch of red king valley potato as an active ingredient, and a pharmaceutical composition for prevention and treatment of gastric ulcer, duodenal ulcer, stomach and duodenal ulcer from Solanum tuberosum L., cv. Redking valley}

The present invention relates to a pharmaceutical composition for the prevention and treatment of gastritis, gastric ulcer and duodenal ulcer containing fresh juice or starch of Solanum tuberosum L. (cv. Redking valley), which is a new variety of potato, as an active ingredient.

Potatoes are the crops of the Andean region of South America and are useful as a wild plant. Potatoes are one of the world's four major food crops after rice, wheat, and corn grown in more than 130 countries because of their short growth period, relatively high production per unit area, and relatively high environmental adaptability. Potatoes are lower in calories than other crops such as rice and barley, while nutrients such as vitamin C, B ₁ , B ₂ , and niacin are nearly vegetable. Therefore, there have been many studies on the physiological activities such as antioxidant activity and general nutritional analysis of potatoes.

Recent changes in the international agricultural environment are placing increasing pressure on the food war or the seed war, and countries are making every effort to protect the variety rights associated with food crops, as food will become a weapon of the future. Korea also joined UPOV, the International Federation for the Protection of Plant Varieties in 2002, and the breed protection period was slightly different from country to country, but at least 18 to 35 years of varietal protection system was legislated. Under these circumstances, the present inventors have developed a variety of functional potato varieties and registered them as national varieties (National Seed Supervisor, 15 new varieties and 4 applications) and foreign potato varieties (Supreme, USA / Atlantic), as well as test cultivation in the United States, Canada, France, Russia, Mongolia, China, Kazakhstan and India.

Potatoes are not only important as stocks in some countries, they are also used to make a variety of processed products, and the consumption of potatoes is increasing even more as the food culture is westernized. This increase in consumption has led to the development of more health-friendly potato varieties.

It is said that potatoes are useful in many kinds of diseases in the private sector. Korean Patent Laid-Open Publication No. 2001-29185 discloses that potato suppresses hypertension, heart disease, constipation, hepatitis and other microorganisms, and thus has antibacterial action and anti-cancer action, but its effect is not known at all.

The present inventors have developed a new variety of potatoes by improving the potatoes, and the new variety potatoes developed by the present inventors have different efficacies for each new variety. That is, Korean Patent Publication No. 628417 discloses that a new variety valley potato (Bora Valley, Juice Valley, Damsom Valley, Gogu Valley) is effective for enhancing the function of the human body. In Korean Patent Registration No. 654231, It has been known that peptides isolated from potatoes of the variety ( Solanum tuberosum L cv. Gogu Valley) are excellent in antimicrobial action, and Korean Patent Publication No. 654232 discloses that peptides isolated from potatoes of Solanum tuberosum L cv. Golden Valley It is known that the peptides are excellent in antibacterial activity. Korean Patent Publication No. 583192 discloses an antioxidant of Solanum tuberosum L. (cv Bora valley) extract of Bora Valley variety, Korean Patent Publication No. 709238 discloses Solanum tuberosum L., cv It is known that the ethanol extract of Bora valley blocks the differentiation of mast cells and has an obesity inhibitory action by decreasing the leptin protein that differentiates into mast cells.

Solanum tuberosum L., cv. Redking valley according to the present invention is a new variety potato developed by the present inventor and has been subjected to a variety protection application (Application No. 2007-438) by the National Seed Supervisor, It is semi-upright type and it is a medium breed, only for autumn cultivation. The shape of tuber is short oval shape, and the color of red color is partial red. This varieties is a new varieties of potatoes which were developed by applying the area test and the test in an area where autumn cultivation is possible after obtaining the true seeds by performing artificial crossing with all red as a model of the Gogu Valley potato.

The inventors of the present invention have found that, in the case of eating potatoes as raw materials, Korean traditional long-term folk remedies have been proved to have excellent effects on gastritis and enteritis. Therefore, the inventors of the present invention have found that the new potatoes Solanum tuberosum L., cv. Redking valley While studying the purpose, the common potatoes do not show any activity on gastritis, gastric ulcer and duodenal ulcer unlike the oral efficacy. Rather, the juice and starch of Red King valley potato are excellent in gastritis, gastric ulcer and duodenal ulcer And completed the present invention.

It is an object of the present invention to provide a novel medicinal use containing as an active ingredient a red king valley potato ( Solanum tuberosum L., cv. Redking valley), which is a new variety of potatoes.

In order to achieve the above object, the present invention provides a pharmaceutical composition for preventing and treating gastritis, gastric ulcer and duodenal ulcer comprising juice or starch of Solanum tuberosum L., cv. Redking valley as an active ingredient .

The present invention can prepare juice using a juicer after removing the shoot parts of Red King valley potato ( Solanum tuberosum L., cv. Redking valley).

The Red King Valley potato starch according to the present invention was prepared by allowing the red king valley potato juice to stand at room temperature for 10 minutes and then filtering it under reduced pressure to separate it into a red king valley potato starch layer and a filtrate and then subjecting the red king valley potato starch layer to freeze decompression Dried to obtain dried starch, or dried at 65 ° C to obtain dried potato starch.

The test in the ethanol-induced gastric ulcer model of red king valley potato juice or starch of the present invention showed an excellent effect of preventing the omeprazole formulation which is commercially available as a preventive and therapeutic agent for gastric ulcer.

In addition, since the present invention exhibits a significant inhibition rate in PGE 2 measurement test of gastric juice of starch or fresh juice of Red King Valley potato, it can be effectively used as a preventive and therapeutic agent for gastritis, gastric ulcer and duodenal ulcer.

The present invention can be administered in various forms of oral and parenteral administration in actual clinical administration, and the most preferable administration route is oral administration. When formulating the composition, it is prepared using a commonly used diluent such as a filler, an extender, a binder, a wetting agent, a disintegrant, a surfactant, or an excipient.

In addition, the dosage or dose of Solanum tuberosum L. cv. Redking valley juice or starch according to the present invention may vary depending on the patient's body weight, age, sex, health condition, diet, administration time, It can be administered in the range of 0.1 - 5 ml / kg for adult juice and 1 mg - 500 mg / kg for starch depending on the severity of disease and disease severity. It is preferable to take it.

Solanum tuberosum L., cv. Redking valley juice or starch of the present invention was found to be non-toxic as a result of the acute toxicity test. Through these safety experiments, the juice and starch of the red king valley potato according to the present invention are considered to have no adverse effect on the human body.

The present invention suppresses the red king valley potato omeprazole formulation with gastric ulcers occur more equal with the fresh juice and starch is commercially available as a preventive and therapeutic agent of gastritis, gastric ulcer and duodenal ulcer (Solanum tuberosum L., cv. Redking valley), PGE 2 Of the total amount of the drug.

Therefore, the present invention can be usefully used as a preventive and therapeutic agent for gastritis, gastric ulcer and duodenal ulcer.

Hereinafter, the present invention will be described in more detail by way of examples of the present invention. The following examples serve to illustrate the present invention and are not intended to limit the scope of the present invention.

≪ Example 1 > Manufacture of Red King Valley potato juice

55 g of Red King valley potato ( Solanum tuberosum L., cv. Redking valley) was washed well and the bud portion was removed. Then, 65 mg of red king valley potato juice was obtained using a juicer.

≪ Example 2 > Manufacture of Red King Valley Potato Starch

50 ml of the red king valley potato juice prepared in Example 1 was left at room temperature for 10 minutes and then filtered under reduced pressure to separate the red king valley potato starch layer and the filtrate. The red king valley potato starch layer was dried at 65 ° C to obtain 8.6 g Of dried potato starch.

≪ Example 3 > Manufacture of Red King Valley Potato Starch

50 ml of the red king valley potato juice prepared in Example 1 was left at room temperature for 10 minutes and then filtered under reduced pressure to separate the red king valley potato starch layer and the filtrate. The red king valley potato starch layer was then freeze-dried under reduced pressure to obtain dried potato starch 9.2 g.

≪ Comparative Example 1 & Manufacture of common potato juice

55 g of general potato was prepared in the same manner as in Example 1 to obtain about 31 ml of common potato juice.

≪ Comparative Example 2 & Production of common potato starch

30 ml of the common potato juice prepared in Comparative Example 1 was prepared in the same manner as in Example 2 to obtain 8.8 g of dried potato starch.

<Experimental Example 1> Inactivation of Potato in Ethanol-Induced Rat Ulcer Model

0.5 ml of potato juice prepared in Examples 1 and 2 and Comparative Examples 1 and 2, and 10 mg of potato starch were each dissolved in 0.1% CMC and SD rats (male, SPF, 150-200 g, purchased from Orient Bios) Orally. After induction of gastric ulcer, ethanol was orally administered after one hour according to the method of Rao et al. (J. Ethnopharmacol. 91, 243, 2004). After 4 hours, the rat was sacrificed and stomach resected. And the width of the ulcer was measured. The results are shown in Table 1. As a control group, 5 mg of omeprazole was used.

As shown in Table 1, it can be seen that Examples 1 and 2 of the present invention exhibited equivalent gastric ulcer inhibitory effects in a comparative test with omeprazole, which is marketed as a preventive and therapeutic agent for gastric ulcer.

However, it can be seen that the comparative example 1 and the comparative example 2 (Superior, USA), which are common potatoes, have little effect, unlike the gastric effects that have been prescribed.

Therefore, fresh juice or starch of the new varieties, Solanum tuberosum L., cv. Redking valley, is very effective against gastritis, gastric ulcer and duodenal ulcer unlike general potato.

<Experimental Example 2> Infectivity in gastritis model

0.5 ml of potato juice prepared in Examples 1 and 3 and Comparative Examples 1 and 2, and 10 mg of potato starch were each dissolved in 0.1% CMC and SD rats (male, SPF, 150-200 g, purchased from Orient Bios) Orally. One hour later, indomethacin was orally administered to SD rats at a dose of 10 mg / rat. After 5 hours, the supernatant was excised, pelleted with a polytron homogenizer, and centrifuged to obtain supernatant. After that, it was extracted with ethyl acetate and dried. (Eur. J. Pharmacol., 425, 153-157, 2001), and then dried in N2, and then analyzed by ELISA for PGE 2 was quantified, and the results are shown in Table 2.

As can be seen from Table 2, Examples 1 and 3 according to the present invention are excellent in PGE2 inhibitory action, but Comparative Examples 1 and 2, which are general potatoes, It can be confirmed that almost no PGE2 inhibitory effect is shown.

Therefore, fresh juice or starch of the new varieties, Solanum tuberosum L., cv. Redking valley, is highly effective against gastritis, gastric ulcer and duodenal ulcer unlike general potato.

< Experimental Example  3> Acute toxicity  Experiment

ICR mouse (male) was tested for acute toxicity according to KFDA guidelines. As a result, the juice of Red King valley ( Solanum tuberosum L., cv. Redking valley) showed no toxicity up to 5.0 ml / kg, and the starch of Solanum tuberosum L., cv. Redking valley No toxicity was observed up to 750 mg / kg.

As such, the juice and starch content of Solanum tuberosum L., cv. Redking valley was practically non-toxic. The effective dose for the above purposes was 0.1 - 5 ml / kg for fresh juice, 1 mg - 500 mg / kg, and it is appropriate to take 1-3 times a day.

Through these safety experiments, the juice and starch of the red king valley potato ( Solanum tuberosum L., cv. Redking valley) Will not adversely affect the human body.

FIG. 1 is a schematic view of a red king valley potato ( Solanum tuberosum L., cv. Redking valley.

Claims (1)

A pharmaceutical composition for the prevention and treatment of gastritis, gastric ulcer or duodenal ulcer comprising, as an active ingredient, a red king valley potato starch layer obtained by separating a filtrate from a juice of a red king valley potato ( Solanum tuberosum L., cv. Redking valley).

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