KR100924023B1 - 효소 연료전지용 전극에의 효소 고정화 방법 - Google Patents
효소 연료전지용 전극에의 효소 고정화 방법 Download PDFInfo
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- KR100924023B1 KR100924023B1 KR1020070115129A KR20070115129A KR100924023B1 KR 100924023 B1 KR100924023 B1 KR 100924023B1 KR 1020070115129 A KR1020070115129 A KR 1020070115129A KR 20070115129 A KR20070115129 A KR 20070115129A KR 100924023 B1 KR100924023 B1 KR 100924023B1
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- Prior art keywords
- enzyme
- fuel cell
- electrode
- anode
- immobilized
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- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01M—PROCESSES OR MEANS, e.g. BATTERIES, FOR THE DIRECT CONVERSION OF CHEMICAL ENERGY INTO ELECTRICAL ENERGY
- H01M8/00—Fuel cells; Manufacture thereof
- H01M8/16—Biochemical fuel cells, i.e. cells in which microorganisms function as catalysts
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E60/00—Enabling technologies; Technologies with a potential or indirect contribution to GHG emissions mitigation
- Y02E60/30—Hydrogen technology
- Y02E60/50—Fuel cells
Abstract
Description
Claims (7)
- 하기 단계들을 포함하는 효소 연료전지용 전극에의 효소 고정화 방법:a) 효소 연료전지용 전극을 시스타민(cystamine)으로 처리하여 아민기(NH2)를 부착하는 단계;b) 상기 전극의 아민기와 퀴논계 전자전달 매개체의 카르복실기(COOH)를 아마이드 결합을 통해 고정화하는 단계;c) 상기 산화반응 효소의 활성부위를 연료기질, 니코틴아마이드 아데닌 디뉴클레오티드(NAD+) 및 염화칼슘(CaCl2)으로 블로킹 시키는 단계; 및d) 상기 고정화된 퀴논계 전자전달 매개체의 또 다른 카르복실기(COOH)와 효소 연료전지용 산화반응 효소의 아민기(NH2)를 아마이드 결합을 통해 고정화하는 단계.
- 삭제
- 제 1항에 있어서, 상기 블로킹은 연료기질 20mM-10M, 니코틴아마이드 디뉴클레오티드(NAD+) 10-60mM 및 염화칼슘 10-60 mM의 농도 하에서 수행하는 것을 특징으로 하는 방법.
- 제 1항에 있어서, 상기 b)단계 및 d)단계에서 아마이드 결합은 커플링제(coupling agent)에 의해 유도되는 것을 특징으로 하는 방법.
- 제 1항에 있어서, 상기 연료기질은 젖산(lactate)이고, 상기 산화반응용 효소는 젖산 탈수소효소(lactate dehydrogenase)이며, 상기 전극에 고정화된 퀴논계 전자전달 매개체는 피롤로퀴놀린 퀴논(pyrroloquinoline quinone)인 것을 특징으로 하는 방법.
- 제 1항에 있어서, 상기 전극은 금으로 된 전극인 것을 특징으로 하는 방법.
- 제 1항 또는 제 3항 내지 제 5항 중 어느 한 항에 따른 고정화 방법으로 효소가 고정화된 효소 연료전지용 전극.
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KR1020070115129A KR100924023B1 (ko) | 2007-11-12 | 2007-11-12 | 효소 연료전지용 전극에의 효소 고정화 방법 |
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KR1020070115129A KR100924023B1 (ko) | 2007-11-12 | 2007-11-12 | 효소 연료전지용 전극에의 효소 고정화 방법 |
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KR20090048986A KR20090048986A (ko) | 2009-05-15 |
KR100924023B1 true KR100924023B1 (ko) | 2009-10-28 |
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Families Citing this family (1)
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KR101255805B1 (ko) * | 2011-04-04 | 2013-04-17 | 경상대학교산학협력단 | 효소 연료 전지용 전극 및 이의 제조 방법 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7018518B2 (en) | 2002-02-04 | 2006-03-28 | Yissum Research Development Company Of The Hebrew University Of Jerusalem | Biosensor carrying redox enzymes |
KR20060129208A (ko) * | 2003-11-05 | 2006-12-15 | 세인트 루이스 유니버시티 | 바이오캐소드 내에 고정화된 효소 |
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Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7018518B2 (en) | 2002-02-04 | 2006-03-28 | Yissum Research Development Company Of The Hebrew University Of Jerusalem | Biosensor carrying redox enzymes |
KR20060129208A (ko) * | 2003-11-05 | 2006-12-15 | 세인트 루이스 유니버시티 | 바이오캐소드 내에 고정화된 효소 |
Non-Patent Citations (2)
Title |
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논문1: J. Am. Chem. Soc* |
도서:Biochemical fuel cells* |
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KR20090048986A (ko) | 2009-05-15 |
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