KR100902263B1 - Method for spot area finding, method and system for DNA microarray analysis - Google Patents

Abstract

The present invention relates to spot area detection methods, DNA microarray analysis methods and systems thereof.

The present invention extracts the spot region in three steps from the DNA microarray image of the target sample obtained by scanning for DNA microarray analysis of the target sample. That is, the spot region excluding noise is extracted from the DNA microarray image, the missing spot region is added based on the coordinate information, and the final spot region is extracted by finally removing the error spot region, and using this, Various values are quantified and corrected for use in genetic analysis.

DNA Chips, DNA Microarrays, Spots, Genetic Analysis

Description

Spot area detection method, DNA microarray analysis method and system {Method for spot area finding, method and system for DNA microarray analysis}

The present invention relates to a spot region detection method, a DNA microarray analysis method and a system thereof, and more particularly, to a spot region detection method of a DNA chip, and a DNA microarray analysis method and system using the same.

DNA chips are the next generation of genetic information agglomerates used to treat intractable diseases caused by genetic abnormalities by densely storing human genetic information and accumulating numerous DNA on glass or plastic substrates.

For the manufacture of such a DNA chip, a technology that allows obtaining a large amount of gene expression information is a DNA microarray technology. DNA microarrays contain hundreds to hundreds of base oligonucleotide probes with known base sequences on solid surfaces such as silicon, surface modified glass, polypropylene, and activated polyacrylamides. It is commonly referred to as microarray by attaching to hundreds of thousands of fixed positions. When the target DNA fragment to be analyzed is bound to the DNA microarray, different hybridization states are generated depending on the complementary degree of the nucleotide sequences on the target DNA fragment and the probes attached to the DNA microarray. Is achieved. Therefore, the nucleotide sequence of the target DNA can be analyzed by observing and interpreting it through an optical method or radiochemical method.

DNA chips manufactured using DNA microarrays can be genetically analyzed with only a small amount of sample by miniaturizing the DNA analysis system, and can simultaneously identify multiple base sequences on the target DNA to provide genetic information quickly and inexpensively. can do. In addition, DNA chips manufactured using DNA microarrays can not only simultaneously analyze vast amounts of genetic information in a short time, but also can identify correlations between genes.

Looking at the method of analyzing a gene using such a DNA chip in the prior art as follows.

First of all, an analysis requires sample pretreatment to take a sample. Sample pretreatment generally uses a polymerase chain reaction (PCR).

Then, the amplified gene, that is, the target sample is attached to the chip (hybridization). In this step, the target sample to be tested is hybridized with the oligo sample containing the information of the gene to the planted chip and attached to the oligo sample having the same sequence. This is done.

Next, the process of removing the target sample remaining on the chip without attaching to the oligo probe is performed, and the image of the chip, that is, the DNA microarray image is read by the scanner to detect how much the target sample is attached to the oligo probe. .

When the DNA microarray image is acquired by scanning, a quantification process for performing statistical analysis is performed to output a quantified value for each spot on the DNA microarray.

Quantified values of each of these spots are used to discern whether the target sample is a patient sample or a normal sample.

 Meanwhile, in the above-described analysis process, various error spots are generated through experiments performed before the quantification process. Therefore, if the statistical analysis is performed after the quantification process based on the wrong information due to the error, spot data that is not properly quantified is joined to the analysis, which reduces the reliability of the analysis. Therefore, before performing the statistical analysis process, the spot detection process of removing the error spot and detecting only the normal spot should be performed.

However, in the conventional spot detection process, the user can detect the number of spots in the entire image, the number of spots in one row, the number of spots in one column, the column and row spacing between the spots and the spots, the maximum and minimum sizes of the spots, and the like. The data has to be entered directly, and even after acquiring the image information, there is a need for a separate manual operation to accurately detect the spot.

SUMMARY OF THE INVENTION The present invention has been made in an effort to provide a method for detecting a spot region without a user's separate input or manual operation, and a method and system for analyzing a microarray of a DNA chip using the same.

Method for detecting a spot region from a DNA microarray image analysis system according to a feature of the present invention for achieving the above object,

Extracting a plurality of spot regions from a plurality of image regions included in the DNA microarray image; Correcting the plurality of spot regions by adding missing spot regions to the plurality of spot regions based on the matrix formed of the plurality of spot regions; And excluding an error spot region from among the corrected plurality of spot regions based on the coordinate information of the plurality of corrected spot regions.

In addition, a method of analyzing a DNA microarray of a target sample by an analysis system according to another aspect of the present invention,

Extracting a plurality of spot regions using expression values of the plurality of pixels included in the image of the DNA microarray; Correcting the plurality of spot areas by adding a spot area to the missing area when there is an area missing from the matrix formed of the plurality of spot areas based on the coordinate information of the plurality of spot areas. ; Excluding an error spot area out of the range of the spot area from the corrected plurality of spot areas based on the coordinate information of the plurality of corrected spot areas; And outputting quantified numerical data by performing a quantification process on the plurality of spots extracted from the image based on the plurality of spot regions from which the error spot region is excluded.

In addition, a medium on which a program for realizing a spot area detection method according to another aspect of the present invention is recorded.

Extracting a plurality of spot regions using expression values of the plurality of pixels included in the image of the DNA microarray; Correcting the plurality of spot areas by adding a spot area to the missing area when there is an area missing from the matrix formed of the plurality of spot areas based on the coordinate information of the plurality of spot areas. ; And excluding an error spot area out of the range of the spot area from the corrected plurality of spot areas based on the coordinate information of the plurality of corrected spot areas.

In addition, the DNA microarray analysis system according to another aspect of the present invention,

An image database for storing a plurality of DNA microarray images including a first DNA microarray image and a second DNA microarray image for a target sample; An image loading unit configured to synthesize the first DNA microarray image and the second DNA microarray image to output a third DNA microarray image; Extracting a plurality of spot regions from the third DNA microarray image, and adding a spot region to a region where a spot region is missing in a matrix consisting of the plurality of spot regions based on coordinate information of the plurality of spot regions; And an image processor configured to correct the plurality of spot regions and extract a final spot region by excluding an error spot region from among the corrected plurality of spot regions based on coordinate information of the corrected plurality of spot regions.

According to an embodiment of the present invention, the DNA microarray analysis system extracts a spot region excluding noise and the like for spot detection, adds a missing spot region to the extracted spot region, and removes an error spot region. By doing so, there is an effect of enabling spot detection without a separate manual operation.

In addition, the above-described spot detection process includes correcting the missing spot area and the error spot area, and enabling the deletion of the spot even after the spot is detected, thereby improving the accuracy of the spot detection used for gene analysis by analyzing the gene. This has the effect of improving performance.

DETAILED DESCRIPTION Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings so that those skilled in the art may easily implement the present invention. As those skilled in the art would realize, the described embodiments may be modified in various different ways, all without departing from the spirit or scope of the present invention. In the drawings, parts irrelevant to the description are omitted in order to clearly describe the present invention, and like reference numerals designate like parts throughout the specification.

Throughout the specification, when a part is said to "include" a certain component, it means that it can further include other components, without excluding other components unless specifically stated otherwise. In addition, terms such as "... unit" described in the specification means a unit for processing at least one function or operation, which may be implemented in hardware or software or a combination of hardware and software.

A DNA microarray analysis system according to an embodiment of the present invention will now be described in detail with reference to the accompanying drawings.

1 illustrates an example of a DNA microarray image of a target sample according to an embodiment of the present invention.

In order to extract spots from the DNA microarray of the target sample, first, a DNA microarray image must be obtained from the target sample. To this end, the target sample (genes of a particular target sample) is stained with two dyes (Cynine dye-Cy3, Cy5, etc.), placed on a DNA chip to induce binding, and then washed and washed with only the gene sequences expressed in the target sample. Experimental procedures to attach to target spots (DNA fragments) on the chip must be preceded. As a result of the experiment, the images scanned by the biochip scanner on the genes expressed in the target spots become DNA microarray images of the target samples.

On the other hand, the dyeing process is performed with two dyes (Cy3, Cy5), and thus two DNA microarray images generated by the two dyes are obtained. That is, two DNA microarray images of one target sample are obtained: a first DNA microarray image obtained through Cy3 staining and a second DNA microarray image obtained through Cy5 staining.

The DNA microarray analysis system described later synthesizes these two DNA microarray images and uses them to detect spot regions. Referring to FIG. 1 as an example, 532.tif (110), which is a first DNA microarray image generated by Cy3 staining, and 635.tif image (120), which is a second DNA microarray image generated by Cy5, are synthesized. The image becomes the third DNA microarray image 130 used for spot area detection.

2 is a structural diagram showing a DNA microarray analysis system 200 according to an embodiment of the present invention.

Referring to FIG. 2, the DNA microarray analysis system 200 includes an interface unit 210, an image database (DataBase, DB) 220, an image loading unit 230, an image processor 240, and a correction unit 250. And result DB 260.

The interface unit 210 receives the user's requirements and outputs them to the image loading unit 230, the image processing unit 240, and the correction unit 250.

The image DB 220 stores a DNA microarray image obtained by scanning the experimental results for the target samples. In this case, two DNA microarray images are stored for each target sample, as described above, the first and second DNA microarray images. Meanwhile, the image DB 220 stores a third DNA microarray image generated by synthesizing the first and second DNA microarray images of each target sample in the image loading unit 230.

When the image loading unit 230 receives an analysis request for a specific target sample through the interface unit 210, the image loading unit 230 reads the first and second DNA microarray images of the target sample from the image DB 220 and performs color correction. And after performing an image overlay process, a third DNA microarray image is generated and output. At this time, the color correction operation and the image synthesis process performed in the image DB 220 is a well-known technique can be easily carried out by those skilled in the art will not be described in detail.

The image processor 240 receives an image of the third DNA microarray from the image loading unit 230 and detects a spot region from a plurality of image regions included in the third DNA microarray image. That is, the image processor 240 determines whether to extract the image area as the spot area by using the intensity, that is, the brightness intensity of each pixel included in each image area. Here, the image region refers to an area composed of a plurality of pixels whose expression value is not zero.

Meanwhile, the image processor 240 calculates coordinate information on the extracted spot regions, and checks and adds missing spot regions based on the coordinate information. Also, the final spot area is extracted by removing an error spot area outside the range where the spot area should be located based on the coordinate information of the spot areas.

The image processor 240 also detects spots from the first and second DNA microarray images based on the final spot region, and performs quantization process on the detected spots to output quantified numerical data. At this time, the quantification process is based on the final spot area, the expression value of the pixels included in each spot recognized from the first and second DNA microarray image, the representative expression value of each spot, the standard deviation of the expression value of each spot And a variety of numerical values, such as Log2Ratio, which is a result of comparing spots included in the first and second DNA microarray images.

The correction unit 250 performs a normalization process on the quantized numerical data output to the image processor 240, thereby correcting the quantized numerical data. Quantified numerical data corrected by the correction unit 250 is stored in the result DB 260. Meanwhile, the normalization process is a process of normalizing the DNA microarray analysis value based on quantified numerical data representing various numerical values calculated in the quantification process. In the genetic analysis method using the DNA chip, several experiments are performed under the same conditions. Even if the experiments are performed under the same conditions and scanning, the DNA chip experiments are very complicated and thus have a high possibility of systematic or unsystematic error. On the side. Therefore, the normalization process is performed to correct this error.

In addition, the correction unit 250 provides the corrected quantified numerical data to the user in the form of various graphs, images, texts, and the like.

Next, the image processor 240 according to an exemplary embodiment of the present invention will be described in detail with reference to FIGS. 3 to 6B.

3 is a structural diagram illustrating an image processor 240 according to an exemplary embodiment of the present invention. 4A illustrates an example of an input image of the spot region extractor 241, and FIG. 4B illustrates an example of an output image of the spot region extractor 241. . 5A illustrates an example of an input image of the spot region corrector 242, and FIG. 5B illustrates an example of an output image of the spot region corrector 242. . 6A illustrates an example of an input image of the error spot region remover 243, and FIG. 6B illustrates an example of an output image of the error spot region remover 243. It is.

Referring to FIG. 3, the image processor 240 includes a spot region extractor 241, a spot region corrector 242, an error spot region remover 243, and a quantizer 244.

The spot region extractor 241 extracts a spot region from the plurality of image regions included in the third DNA microarray image. To this end, the spot region extractor 241 detects an image region consisting of pixels having an expression value equal to or greater than a set expression value, and extracts an image region having a set size or more from among the plurality of detected image regions as a spot region. When the spot region is extracted, the spot region extractor 241 outputs spot region information including coordinate information of the extracted spot region, the size of the spot region, and the like. In this case, the coordinate information of the spot area may include the number of spot areas included in each row and the number of spot areas included in each column, and the column and row coordinates of each spot area. Include.

4A and 4B, FIG. 4A is a third DNA microarray image input to the spot region extractor 241, and image regions including noise are arranged. FIG. 4B illustrates a spot region extracted from the image region included in the third DNA microarray image by the spot region extractor 241 excluding an image region corresponding to noise. The extracted plurality of spot regions is illustrated in FIG. 4B. As shown, they are arranged in a matrix. Accordingly, the extracted coordinate information of each spot may be represented by using a method of representing coordinates in a matrix.

The spot region corrector 242 adds missing spot regions using the spot region information output from the spot region extractor 241. To this end, the spot region correction unit 242 checks the position where the spot region should exist by using the spot regions arranged in a matrix form based on the coordinate information of the spot regions included in the spot region information. If there is no spot area where it should be, add a new spot area to the missing area.

5A and 5B, FIG. 5A is an image illustrating a spot region extracted by the spot region extractor 241, and FIG. 5B is a spot region after a spot region is newly added by the spot region corrector 242. An image representing. According to the coordinate information, since the empty space at the bottom of FIG. 5A in the matrix arrangement is the area where the original spot area should be located, the spot correction unit 242 adds a new spot area as shown in FIG. 5B. In FIG. 5B, the newly added spot area is indicated by a white circle. When the missing spot area is added, the spot area corrector 242 outputs spot area information on the corrected spot area. In this case, the corrected spot region information includes coordinate information, size information, etc. of all the extracted spot regions including the spot region added by the spot region corrector 242.

The error spot region remover 243 receives the corrected spot region information output from the spot region corrector 242, and determines that the spot region exists outside the range where the spot region should exist as the error spot region. Remove from spot area. To this end, the error spot region remover 243 uses a characteristic in which a spot region having the same row coordinates and a spot region having the same column coordinates exist for a single spot region at a predetermined number (for example, ten or more). That is, the error spot area remover 243 may have a smaller number of spot areas having the same row coordinates as the number of spot areas having the same row coordinates based on the coordinate information corrected and output by the spot area corrector 242. The spot area is determined as an error spot area existing outside the range of the spot area, and the final spot area is determined by excluding this from the spot area.

6A and 6B, FIG. 6A is an image illustrating a spot area including an error spot area out of a spot area range, and FIG. 6B is an error spot area removed by the error spot area remover 243. Is an image representing the final spot area. In the upper left of FIG. 6A, an image area outside the range of the spot area is displayed as a spot area. In FIG. 6B, the spot area display of the image area is removed. Here, the DNA microarray analysis system 200 displays the area determined as the spot area with a circular border.

The quantization unit 244 performs a quantification process on the first and second DNA microarray images based on the spot area information of the final spot area selected by the error spot area removal unit 243 to represent representative expressions of each spot extracted. Output quantified numerical data such as values.

Next, the DNA microarray analysis method according to the embodiment of the present invention will be described in detail with reference to FIG. 7.

7 is a flowchart illustrating a DNA microarray analysis method of the DNA microarray analysis system 200 according to an exemplary embodiment of the present invention.

First, the DNA microarray analysis system 200 stores the first and second DNA microarray images scanned from the DNA microarrays of the target samples in the image DB 220, and stores the target samples to be analyzed at the request of the user. The first and second DNA microarray images are read from the image DB 220 (S101). In addition, the DNA microarray analysis system 200 performs a color correction operation and an image overlay operation on the first and second DNA microarray images read from the image DB 220 to be used for spot area detection. Generate DNA microarray images. At this time, the color correction operation and the image synthesis process performed are well known techniques, and thus can be easily implemented by those skilled in the art.

Thereafter, the DNA microarray analysis system 200 detects the spot region from the third DNA microarray image through the image processor 240 (S102).

When the spot region detection is completed, the image processor 240 detects spots from the first and second DNA microarray images based on the detected spot region, performs a quantification process, and outputs quantified numerical data (S103). .

After the quantification process, the DNA microarray analysis system 200 outputs the corrected numerical data by performing a normalization process for the numerical data quantified by the correction unit 250 (S104).

After the normalization process, the DNA microarray analysis system 200 stores the numerical data corrected through the correction unit 250 in the result DB 260, and reports the report, scatter plot, and image ( image) to the user in the form (S105).

8 is a flowchart illustrating a spot detection method of the DNA microarray analysis system 200 according to an exemplary embodiment of the present invention.

Referring to FIG. 8, the image processor 240 first extracts a spot region from an input third DNA microarray image. In this case, the image processor 240 selects an image region including pixels having a set expression value or more from among the plurality of image regions included in the third DNA microarray image, and sets an image region having a size larger than or equal to the set region among the selected image regions as a spot region. Extract (S201). When the spot region is extracted, the image processor 240 calculates spot region information including coordinate information of the entire spot regions, the size of each spot region, and the like.

Thereafter, the image processor 240 adds the missing spot area in addition to the spot area extracted based on the coordinate information included in the spot area information. To this end, the image processor 240 identifies the location where the spot area should exist by using the spot areas arranged in a matrix form based on the coordinate information. Thereafter, when the spot area is missing at the position where the spot area should exist, the image processor 240 adds the spot area to the missing area (S202).

Thereafter, the image processor 240 corrects the spot region information to include coordinate information of the corrected spot region after newly adding the spot region.

In addition, the image processor 240 determines the spot area that exists outside the range of the spot area as an error spot area based on the corrected coordinate information, and excludes the spot area from the spot area (S203).

As described above, the DNA microarray analysis system 200 detects the spot region in three steps through the image processor 240 and detects the spot based on the detected spot region. Such a spot detection method enables spot detection without a user's separate manual operation so that spots can be detected efficiently. In addition, in three steps, the spot area is separated from the background, and the spot area and the error spot area are corrected, thereby increasing the accuracy of spot detection.

Figure 9 shows an example of a report showing the results of DNA microarray analysis according to an embodiment of the present invention.

Referring to FIG. 9, the report includes a first graph 310 showing a Log2Ratio distribution of all chromosomes of a target sample, a second graph 320 showing Log2Ratio_Mean values of all chromosomes, and spot information indicating information about spots. (Spot Information) 330. In this case, the spot information shown is a part showing information on the selected circle and the selected spot when one circle is selected in the first graph 310.

Meanwhile, the DNA microarray analysis system 200 may delete specific spots among the detected spots at the request of the user, in addition to showing the analysis result of the DNA microarray. For example, when a specific spot is selected in the first graph 310 of FIG. 8 and a deletion request is input, the DNA microarray analysis system 200 deletes it from the spot and also stores the analysis result stored in the result DB 260. Update

As described above, the DNA microarray analysis system 200 further includes a function of receiving a deletion input for an error spot and thus eliminating the error spot, thereby further improving the analysis performance of the DNA microarray. have.

Embodiments of the present invention are not implemented only through the above-described apparatus and / or method, but may be implemented through a program for realizing a function corresponding to the configuration of the embodiments of the present invention, a recording medium on which the program is recorded, and the like. Such implementations may be readily implemented by those skilled in the art from the description of the above-described embodiments.

Although the embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements of those skilled in the art using the basic concepts of the present invention defined in the following claims are also provided. It belongs to the scope of rights.

1 is a view showing an example of a DNA microarray image according to an embodiment of the present invention.

2 is a structural diagram showing a DNA microarray analysis system according to an embodiment of the present invention.

3 is a structural diagram illustrating an image processor according to an exemplary embodiment of the present invention.

4A illustrates an example of an input image of the extractor according to an exemplary embodiment of the present invention.

4B illustrates an example of an output image of the extractor according to an exemplary embodiment of the present invention.

5A illustrates an example of an input image of a correction unit according to an exemplary embodiment of the present invention.

5B illustrates an example of an output image of a corrector according to an exemplary embodiment of the present invention.

6A illustrates an example of an input image of an error spot remover according to an exemplary embodiment of the present invention.

6B illustrates an example of an output image of an error spot remover according to an exemplary embodiment of the present invention.

7 is a flowchart illustrating a DNA microarray analysis method of a DNA microarray analysis system according to an embodiment of the present invention.

8 is a flowchart illustrating a spot detection method of a DNA microarray analysis system according to an exemplary embodiment of the present invention.

Figure 9 shows an example of a report showing the results of DNA microarray analysis according to an embodiment of the present invention.

Claims (15)

A method of detecting a spot region from a DNA microarray image by an analysis system, Extracting a plurality of image regions including pixels having an expression value equal to or greater than a set expression value among a plurality of image regions included in the DNA microarray image; Extracting an image area having a predetermined size or more among the extracted plurality of image areas into a plurality of spot areas; Correcting the plurality of spot regions by adding missing spot regions to the plurality of spot regions based on the matrix formed of the plurality of spot regions; And Excluding an error spot region from among the corrected plurality of spot regions based on coordinate information of the plurality of corrected spot regions; Spot area detection method comprising a. delete The method of claim 1, The correcting step, Calculating the number of spot regions by row and the number of spot regions by column in the matrix; And Adding the missing spot area to the missing area when there is an area in which the spot area is missing in the matrix based on the number of spot area by row and the number of spot area by column Spot area detection method comprising a. The method of claim 1, Excluding the step, Calculating row coordinates and column coordinates of the corrected plurality of spot regions; And Among the corrected spot areas, the spot area having the same row coordinates as its own is less than a first set value or the number of spot areas having the same column coordinates as its own is less than a second set value. Step to select Spot area detection method comprising a. A method of analyzing a DNA microarray of a target sample by an analysis system, Extracting a plurality of spot regions using expression values of the plurality of pixels included in the image of the DNA microarray; Correcting the plurality of spot areas by adding a spot area to the missing area when there is an area missing from the matrix formed of the plurality of spot areas based on the coordinate information of the plurality of spot areas. ; Based on the coordinate information of the corrected plurality of spot regions, excluding an error spot region outside the range of the spot region from the corrected plurality of spot regions; And Outputting quantified numerical data by performing a quantification process on a plurality of spots extracted from the image based on the plurality of spot regions from which the error spot region is excluded; DNA microarray analysis method comprising a. The method of claim 5, Correcting the quantified numerical data through a normalization process and outputting analysis data of the DNA microarray; DNA microarray analysis method further comprising. The method of claim 5, Scanning the DNA microarray to obtain a first image generated by staining the target sample with cy3 and a second image generated by staining the target sample with cy5; And Synthesizing the first image and the second image to generate the image DNA microarray analysis method further comprising. The method of claim 5, The correcting step, Calculating coordinate information of the plurality of spot regions; Coordinate information of the plurality of spot regions, DNA microarray analysis method comprising the number of spot regions for each row and the number of spot regions for each column of the matrix. The method of claim 5, Excluding the step, Calculating coordinate information of the corrected plurality of spot regions; And the coordinate information of the corrected plurality of spot regions includes row and column coordinates of the corrected plurality of spot regions. In the analysis system, Extracting a plurality of spot regions using expression values of the plurality of pixels included in the image of the DNA microarray; Correcting the plurality of spot areas by adding a spot area to the missing area when there is an area missing from the matrix formed of the plurality of spot areas based on the coordinate information of the plurality of spot areas. step; And Based on the coordinate information of the plurality of corrected spot regions, excluding an error spot region that is out of the range of the spot region from the corrected plurality of spot regions; And a program for recording a spot area detection method. An image database for storing a plurality of DNA microarray images including a first DNA microarray image and a second DNA microarray image for a target sample; An image loading unit configured to synthesize the first DNA microarray image and the second DNA microarray image to output a third DNA microarray image; And Extracting a plurality of spot regions from the third DNA microarray image, and adding the spot regions to the regions where the spot regions are missing in the matrix consisting of the plurality of spot regions based on the coordinate information of the plurality of spot regions. An image processing unit for correcting the spot area of the extracted area and extracting the final spot area by excluding an error spot area from the corrected spot areas based on the coordinate information of the corrected spot areas; DNA microarray analysis system comprising a. The method of claim 11, The image processor, Extracting a plurality of image regions consisting of pixels equal to or greater than a set expression value among the plurality of pixels included in the third DNA microarray image, and extracting an image region equal to or greater than a set size among the extracted plurality of image regions as the plurality of spot regions. A spot region extractor; A spot area corrector configured to calculate coordinate information of the plurality of spot areas, and correct the plurality of spot areas by checking the missing area based on the coordinate information of the plurality of spot areas; And An error spot region removal unit configured to calculate coordinate information of the plurality of corrected spot regions and determine the error spot region based on the corrected coordinate information of the plurality of spot regions and exclude the corrected spot region from the corrected plurality of spot regions. DNA microarray analysis system comprising a. The method of claim 12, The image processor, A quantifier extracting a plurality of spots from the first DNA microarray image and the second DNA microarray image based on the final spot region, and performing quantification on the plurality of spots to output quantified numerical data DNA microarray analysis system further comprising. The method of claim 13, A correction unit for correcting the quantified numerical data through a normalization process and outputting the corrected quantified numerical data in at least one of an image, a graph, and text DNA microarray analysis system further comprising. The method of claim 12, The coordinate information includes the number of spot regions by row and the number of spot regions by column of the matrix, The coordinate information of the plurality of corrected spot regions includes a row coordinate and a column coordinate of each spot region included in the corrected plurality of spot regions.

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