KR100878335B1 - Corynebacterium glutamicum genes encoding stress, resistance and tolerance proteins - Google Patents

Abstract

The present invention relates to an isolated nucleic acid molecule referred to as an SRT nucleic acid molecule encoding a novel SRT protein from Corynebacterium glutamicum. The present invention also provides an antisense nucleic acid molecule, a recombinant expression vector comprising an SRT nucleic acid molecule, and a host cell into which the expression vector is introduced. The present invention also improves the production of the target compound from the organism based on genetic engineering of the isolated SRT protein, mutant SRT protein, fusion protein, antigenic peptide, and SRT gene in Corynebacterium glutamicum. It provides a method to make.

Corynebacterium glutamicum, SRT protein, fine chemicals, stress

Description

Corynebacterium glutamicum gene encoding stress resistant and resistant proteins {CORYNEBACTERIUM GLUTAMICUM GENES ENCODING STRESS, RESISTANCE AND TOLERANCE PROTEINS}

Related Applications

This application is a copy of a previously filed US Provisional Patent Application No. 60/141031 (filed June 25, 1999), US Provisional Patent Application No. 60/142692 (filed July 1, 1999) and US Provisional Patent Application; Claim priority to 60/151214, filed August 27, 1999. The present application is also filed in German Patent Application No. 199304 29.7 (filed Jul. 1, 1999), German Patent Application No. 1931413.6 (filed Jul. 8, 1999), and German Patent Application No. 19931457.8 (July 8, 1999). ), German Patent Application No. 1931541.8 (filed July 8, 1999), German Patent Application No. 1932209.0 (filed July 9, 1999), German Patent Application No. 19932230.9 (filed July 9, 1999), Priority to German Patent Application No. 19932914.1 (filed Jul. 14, 1999), German Patent Application No. 19940764.9 (filed August 27, 199) and German Patent Application No. 19941382.7 (filed August 31, 1999) Charges. The entire contents of all the above applications are expressly included in the present specification.

Some products and by-products of naturally occurring metabolic processes in cells are used in a variety of industries, including the food, feed, cosmetic and pharmaceutical industries. These molecules, collectively referred to as "fine chemicals", include organic acids, proteomic and nonproteinaceous amino acids, nucleotides and nucleosides, lipids and fatty acids, diols, carbohydrates, aromatic compounds, vitamins and cofactors, and enzymes. Their production is most conveniently carried out through large-scale cultivation of bacteria developed to produce and secrete large quantities of specific molecules of interest. Particularly useful organisms for this purpose are Corynebacterium glutamicum, a gram positive nonpathogenic bacterium. Through strain selection, a number of mutant strains have been developed that produce a series of desirable compounds. However, the selection of improved strains for the production of certain molecules is a time consuming and difficult process.

The present invention provides novel bacterial nucleic acid molecules with various uses. These uses include identification of microorganisms that can be used in the production of fine chemicals as reference points and transformation markers for mapping the genome of Corynebacterium glutamicum; Regulating the production of fine chemicals in Corynebacterium glutamicum or related bacteria; And typing or identification of Corynebacterium glutamicum or related bacteria. These new nucleic acid molecules encode proteins, referred to herein as stress resistance and resistance (SRT) proteins.

 Corynebacterium glutamicum is a Gram-positive aerobic bacterium commonly used industrially for the mass production of various fine chemicals, for the decomposition of hydrocarbons (eg mineral oil spills) and for the oxidation of terpenoids. Thus, the SRT nucleic acid molecules of the present invention can be used to identify microorganisms that can be used, for example, to produce fine chemicals by fermentation processes. The sequence variation of can be used to control the production of one or more fine chemicals from microorganisms (e.g., to improve the production or production of one or more fine chemicals from Corynebacterium or Brevibacterium species. To do so).

The SRT nucleic acid of the present invention identifies an organism that may be Corynebacterium glutamicum or a microorganism closely related thereto, or detects the presence of Corynebacterium glutamicum or a microorganism closely related thereto in a mixed microbial population. Can also be used to verify. The present invention exists by probing the extracted genomic DNA in a culture of the same or mixed microbial population with probes spanning the region of the Corynebacterium glutamicum gene present only in Corynebacterium glutamicum under stringent conditions. Nucleic acid sequences of many Corynebacterium glutamicum genes can be identified. Although Corynebacterium glutamicum itself is not pathogenic, it is associated with species pathogenic to humans, such as Corynebacterium diphtheria (pathogen of diphtheria), and the detection of such organisms is of clinical importance.

The SRT nucleic acid molecules of the present invention may also serve as a reference point for mapping the genome of Corynebacterium glutamicum or the genome of related organisms. Similarly, these molecules, variants or portions thereof, can serve as markers for genetically engineered Corynebacterium or Brevibacterium species.

SRT proteins encoded by the novel nucleic acid molecules of the present invention may allow Corynebacterium glutamicum to survive under conditions that are chemically or environmentally harmful to the microorganism, for example. The availability of cloning vectors for use in Corynebacterium glutamicum, such as the cloning vectors disclosed in US Pat. No. 4,649,119 (Sinskey et al.); And genetic engineering techniques of Corynebacterium glutamicum and related Brevibacterium species (eg, lactofermentum) (Yoshihama et al, J. Bacteriol. 162: 591-597 (1985); Katsumata et al., J Bacteriol. 159: 306-311 (1984); and Santamaria et al., J. Gen. Microbiol. 130: 22372246 (1984)), the nucleic acid molecule of the present invention is an environment that normally interferes with the proliferation of this organism. For example, such organisms may be genetically engineered to be more efficient producers of one or more fine chemicals through the ability of these proteins to allow the growth and replication of Corynebacterium glutamicum under conditions that often occur during large fermentation growth. Can be used to manipulate For example, by overexpressing or genetically engineering a heat-shock inducing protease molecule to optimize its activity, one can increase the bacteria's ability to break down correctly folded proteins when they are exposed to high temperatures. By lowering the number of misfolded (and possibly misregulated or nonfunctional) proteins in the cell that interfere with normal reaction mechanisms, the ability of cells to function normally in such cultures is increased to provide increased viability. This overall increase in the number of cells with higher viability and activity also increases the yield, productivity and / or production efficiency of one or more desired fine chemicals, which at least produce these chemicals in the culture. This is because the number of cells is relatively higher.

The present invention provides, for example, novel SRT nucleic acid molecules encoding SRT proteins that can allow Corynebacterium glutamicum to survive under chemically or environmentally harmful conditions to Corynebacterium glutamicum. .

Nucleic acid molecules encoding SRT proteins are referred to herein as SRT nucleic acid molecules. In a preferred embodiment, the SRT protein participates in metabolic pathways that can allow Corynebacterium glutamicum to survive under conditions that are chemically or environmentally harmful to Corynebacterium glutamicum. Examples of such proteins are proteins encoded by the genes listed in Table 1.

Thus, one aspect of the invention relates to isolated nucleic acid molecules (eg, cDNA, DNA or RNA) comprising SRT proteins or nucleotide sequences encoding bioactive proteins thereof, as well as SRT encoding nucleic acids (eg, DNA or nucleic acid fragments suitable as primers or hybridization probes for the detection or amplification of mRNA). In a particularly preferred embodiment, the isolated nucleic acid molecule is one of the nucleotide sequences described as odd numbered sequences in the sequence listing (eg, SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7 ...), or these nucleotide sequences One of the coding regions or its complement. In another particularly preferred embodiment, an isolated nucleic acid molecule of the invention is a nucleotide sequence described as an odd numbered sequence (e.g., SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7 ...) in the sequence listing or At least about 50%, preferably at least about 60%, more preferably at least about 70%, 80% or 90%, even more preferably at least about 95%, 96%, 97%, 98% , Nucleotide sequences having at least 99% or more homology. In another preferred embodiment, the isolated nucleic acid molecule encodes one of the amino acid sequences described by the even-numbered sequences in the sequence listing (eg, SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8 ....). Preferred SRT proteins of the invention also preferably have at least one of the SRT activities described herein.

In other embodiments, an isolated nucleic acid molecule encodes a protein or portion thereof, wherein the protein or portion thereof comprises an amino acid sequence having sufficient homology with an amino acid sequence of the invention (eg, an even number sequence in the sequence listing). Sequence), for example, an amino acid sequence having sufficient homology with the amino acid sequence of the present invention such that the protein or a portion thereof can maintain SRT activity. Preferably, the protein or portion thereof encoded by the nucleic acid molecule maintains the ability to increase the survival of the microorganism under conditions that are chemically or environmentally harmful to Corynebacterium glutamicum. In one embodiment, the protein encoded by the nucleic acid molecule is at least about 50%, preferably at least about 60%, of an amino acid sequence of the invention (eg, an entire amino acid sequence selected from those having even-numbered sequences in the sequence listing) , More preferably at least about 70%, 80% or 90%, most preferably at least about 95%, 96%, 97%, 98% or 99%, or more homologous. In another preferred embodiment, the protein is an open reading frame as represented by the entire amino acid sequence of the invention (the corresponding odd numbered sequence in the sequence listing (e.g., SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7 ...). Is a full-length Corynebacterium glutamicum protein substantially homologous to).

In another preferred embodiment, the isolated nucleic acid molecule is from Corynebacterium glutamicum and is at least about 50% with one of the amino acid sequences of the invention (eg, one of even numbered sequences in the sequence listing). Or a biologically active domain having more than one homology and having the ability to increase the survival of Corynebacterium glutamicum under conditions chemically or environmentally harmful to the microorganism or having one or more of the activities listed in Table 1. And encode a protein (eg, an SRT fusion protein) that also comprises a heterologous nucleic acid sequence encoding a heterologous polypeptide or regulatory region.

In other embodiments, an isolated nucleic acid molecule hybridizes under stringent conditions with a nucleic acid molecule that is at least 15 nucleotides in length and comprises a nucleotide sequence of the invention (eg, an odd numbered sequence in the sequence listing). Preferably, the isolated nucleic acid molecule corresponds to a naturally occurring nucleic acid molecule. More preferably, the isolated nucleic acid encodes the SRT protein of Corynebacterium glutamicum or a biologically active portion thereof.

Another aspect of the invention relates to vectors comprising nucleic acid molecules of the invention, such as recombinant vectors, and host cells into which such vectors are introduced. In one embodiment, the host cell is used to produce the SRT protein by culturing the host cell in a suitable medium. The SRT protein can then be isolated from the medium or host cell.

Another aspect of the invention relates to a genetically modified microorganism into which the SRT gene is introduced or mutated. In one embodiment, the genome of the microorganism is mutated by introducing a nucleic acid molecule of the invention encoding a wild type or mutant SRT sequence as a transgene. In other embodiments, the endogenous SRT gene in the genome of the microorganism is mutated, eg, functionally disrupted by homologous recombination with the mutated SRT gene. In other embodiments, the endogenous or transgenic SRT gene in the microorganism is modified by one or more point mutations, deletions or inversions, but still encodes a functional SRT protein. In other embodiments, one or more regulatory regions (eg, promoters, repressors, or inducers) of the SRT gene in a microorganism (eg, deletion, truncation, inversion) Or by point mutations) to regulate the expression of the SRT gene. In a preferred embodiment, the microorganism belongs to the genus Corynebacterium or Brevibacterium, particularly preferably Corynebacterium glutamicum. In a preferred embodiment, the microorganism is also used for the production of the desired compound, such as amino acids, particularly preferably lysine.

In another aspect, the invention provides a method of confirming the presence or activity of Corynebacterium diphtheria in one subject. This method involves detecting one or more nucleic acid or amino acid sequences of the invention in one subject (eg, the sequences listed in the sequence listing as SEQ ID NOS: 1-304), thereby preventing the presence or activity of Corynebacterium diphtheria in the subject. Can be detected.

Another aspect of the invention relates to an isolated SRT protein or portion thereof, eg, a biologically active site thereof. In a preferred embodiment, the isolated SRT protein or part thereof possesses the ability to increase the survival of Corynebacterium glutamicum under conditions that are chemically or environmentally harmful to this microorganism. In another preferred embodiment, the isolated SRT protein or portion thereof is sufficient to maintain the ability to increase the survival of Corynebacterium glutamicum under conditions that are chemically or environmentally harmful to this microorganism. (Even numbered sequence in the sequence listing) is sufficient homology.

The present invention provides an isolated formulation of SRT protein. In a preferred embodiment, the SRT protein comprises an amino acid sequence of the invention (eg, an even numbered sequence in the sequence listing). In another preferred embodiment, the invention relates to an isolated full-length protein (corresponding odd-numbered sequence in the sequence listing) that is substantially homologous to the entire amino acid sequence (even-numbered sequence in the sequence listing) of the present invention. Coded by). In another embodiment, the protein is at least about 50%, preferably at least about 60%, more preferably at least about 70%, 80 with the entire amino acid sequence of the invention (eg, even numbered sequences in the sequence listing) At least% or 90%, most preferably at least about 95%, about 96%, about 97%, about 98% or about 99%, or more homologous. In other embodiments, an isolated SRT protein comprises an amino acid sequence that is at least about 50% homologous to one of the amino acid sequences of the invention (eg, an even numbered sequence in the sequence listing), It can increase the survival rate of Corynebacterium glutamicum under chemically or environmentally harmful conditions, or have one or more activities listed in Table 1.

Alternatively, the isolated SRT protein may hybridize with the nucleotide sequence of one of the even-numbered sequences listed in the sequence listing, for example, under stringent conditions, or at least about 50%, preferably at least about 60%, more preferably Is encoded by at least about 70%, 80% or 90%, most preferably at least about 95%, about 96%, about 97%, about 98% or about 99%, or more homologous nucleotide sequences May comprise an amino acid sequence. It is also preferred that the preferred form of the SRT protein is one or more SRT bioactivity described herein.

The SRT polypeptide or its biologically active site can be operatively linked to a polypeptide other than the SRT polypeptide to form a fusion protein. In a preferred embodiment, the activity of this fusion protein differs from that of the SRT protein alone. In a preferred embodiment, this fusion protein increases the yield, productivity and / or production efficiency of the desired fine chemical from Corynebacterium glutamicum. In a particularly preferred embodiment, this fusion protein is introduced into a host cell to regulate the production of the desired compound from the host cell.

In another aspect, the invention provides a method for screening a molecule that modulates the activity of the SRT protein by interacting with the SRT protein itself, a substrate or binding partner of the SRT protein, or by regulating the transcription or translation of the SRT nucleic acid molecule of the invention. To provide.

Another aspect of the present invention is directed to a process for the manufacture of fine chemicals. This method involves culturing cells comprising a vector expressing the SRT nucleic acid molecule of the present invention to produce fine chemicals. In a preferred embodiment, the method also comprises transfecting the cells with a vector expressing the SRT nucleic acid to obtain a cell containing said vector.

In another preferred embodiment, the method also includes recovering fine chemicals from the culture. In particularly preferred embodiments, the cells are from the Corynebacterium or Brevibacterium genus or selected from the strains described in Table 3.

Another aspect of the invention relates to a method of controlling the production of molecules from microorganisms. Such methods include contacting cells with a substance that modulates SRT protein activity or SRT nucleic acid expression such that cell related activity is changed relative to the activity in the absence of the substance. In a preferred embodiment, the cells are adjusted to be resistant to one or more toxic chemicals or environmental stresses to improve the yield, productivity and / or production efficiency of the desired fine chemicals from this microorganism. A substance that modulates SRT protein activity may be a substance that stimulates SRT protein activity or SRT nucleic acid expression. Examples of substances that stimulate SRT protein activity or SRT nucleic acid expression include small molecules, active SRT proteins, and nucleic acids encoding SRT proteins introduced into cells. Examples of substances that inhibit SRT activity or expression include small molecules and antisense SRT nucleic acid molecules.

Another aspect of the invention relates to a method of controlling the yield of a desired compound from a cell, comprising introducing a wild-type or mutant SRT gene into a cell such that it is maintained on a separate plasmid or introduced into the genome of a host cell. It is about. Insertion into the genome (such insertion can be random or can be caused by homologous recombination that allows the native gene to be replaced by an introduced copy) regulates the production of the desired compound from the cell. In a preferred embodiment, the yield is increased. In another preferred embodiment, the chemical is a fine chemical. In a particularly preferred embodiment, the fine chemical is an amino acid. In a particularly preferred embodiment said amino acid is L-lysine.

The present invention provides SRT nucleic acid and protein molecules involved in the survival of this microorganism when Corynebacterium glutamicum is exposed to chemical or environmental risks. The molecules of the present invention can be used to control the production of fine chemicals from microorganisms, which corynebacterium is in the presence of toxic chemicals or harmful environmental conditions such as these SRT proteins may encounter by chance during large expression propagation. This is because it provides a means for the continuous growth and proliferation of glutamicum. In the absence of toxicity, it is possible to increase the yield, productivity and / or production efficiency of one or more fine chemicals obtained from these cultures by increasing the growth rate or maintaining at least normal growth under insufficient conditions, which is at least a culture. Because of the relatively large number of cells producing fine chemicals. Aspects of the invention are described in more detail below.

I. Fine Chemicals

The term "fine chemical" is well known in the art and includes molecules produced by organisms used in a variety of industries, such as, but not limited to, the pharmaceutical, agricultural and cosmetic industries. Such compounds include organic acids such as tartaric acid, itaconic acid and diaminopimelic acid, proteomic and nonproteinaceous amino acids, purine and pyrimidine bases, nucleosides, and nucleotides [see, eg, Kuninaka, A. (1996) Nucleotides and related compounds, p. 561-612, in Biotechnology vol. 6, Rehm et al., Eds.VCH: Weinheim, the content of which is incorporated herein), lipids, saturated and unsaturated Fatty acids (eg arachidonic acid), diols (eg propanediol and butanediol), carbohydrates (eg hyaluronic acid and trehalose), aromatic compounds (eg aromatic amines, vanillin and indigo), vitamins and auxiliary Factors (Ullmann's Encyclopedia of Industrial Chemistry, vol. A27, "Vitamins", p. 443-613 (1996) VCH: Weinheim and references therein; and Ong, AS, Niki, E. & Packer, L. (1995) "Nutrition, Lipids, Health, and Disease" Proceedings of the UNESCO / Confederation of Scientific and Technological Associations in Malaysia, and the Society for Free Radical Research Asia, held Sept. 1-3, 1994 at Penang, Malaysia, AOCS Press, (1995)], enzymes, poly Keted (Cane et al. (1998) Science 282: 63-68), and Gutcho (1983) Chemicals by Fermentation, Noyes Data Corporation, ISBN: 0818805086) and all other chemicals described therein. . The metabolism and use of some of these fine chemicals will be described in more detail below.

A. Amino Acid Metabolism and Uses

Amino acids make up the basic structural unit of every protein and are essential for normal cellular function in all organisms. The term "amino acid" is recognized in the art. Of the amino acids, 20 of them are proteinogenic amino acids and act as structural units for the proteins to which they are bound by peptide bonds, while nonproteinogenic amino acids (hundreds of them are known) are not normally found in proteins. Ulmann's Encyclopedia of Industrial Chemistry, vol. A2, p. 57-97 VCH: See Weinheim (1985). Although L-amino acids are generally the only form found in naturally occurring proteins, amino acids can be either D-optical or L-optical structures. The biosynthesis and degradation pathways of each of the 20 proteinogenic amino acids are well characterized in both prokaryotic and eukaryotic cells (see, eg, Stryer, L. Biochemistry, 3rd edition, pages 578-590 (1988)). ). Amino acids called "essential" amino acids (histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, and valine) are common nutritional requirements due to the complexity of biosynthesis. Easily converted to amino acids (alanine, arginine, asparagine, aspartate, cysteine, glutamate, glutamine, glycine, proline, serine and tyrosine). Higher animals have the ability to synthesize some of the essential amino acids, but these essential amino acids must be supplied from meals in order for normal protein synthesis to occur.

In addition to the function of amino acids in protein biosynthesis, these amino acids are themselves interesting chemicals and many amino acids have been found to be used in a variety of applications in the food, feed, chemical, cosmetic, agricultural and pharmaceutical industries. Lysine is an important amino acid not only in humans but also in the nutrition of unit animals such as poultry and swine. Glutamate is most commonly used as a flavourant additive (mono-sodium glutamate, MSG), and aspartate, phenylalanine, glycine and citrate are also widely used throughout the food industry. Glycine, L-methionine and tryptophan are all used in the pharmaceutical industry. Glutamine, valine, leucine, isoleucine, histidine, arginine, proline, serine and alanine are used in both the pharmaceutical and cosmetic industries. Threonine, tryptophan and D / L-methionine are common feed additives (Leuchtenberger, W. (1996) Amino aids technical production and use, p. 466-502 in Rehm et al. (Eds.) Biotechnology vol. 6, chapter 14a , VCH: Weinheim). In addition, these amino acids are synthetic amino acids and proteins such as N-acetylcysteine, S-carboxymethyl-L-cysteine, (S) -5-hydroxytryptophan, and Ulmann's Encyclopedia of Industrial Chemistry, vol. A2, p. 57-97, VCH: Weinheim, 1985), have been found to be useful as precursors for the synthesis of others.

The characterization of the biosynthesis of these natural amino acids in organisms capable of producing these natural amino acids, such as bacteria, is well characterized. 47: 533-606). Glutamate is synthesized by reduction amination of α-ketoglutarate, which is an intermediate of the citric acid cycle. Glutamine, proline and arginine are each produced from glutamate. Biosynthesis of serine begins with 3-phosphoglycerate (an intermediate of its action) and is a three step process of producing serine after oxidation, transaminoation and hydrolysis steps. Both cysteine and glycine are produced from serine, which is produced by the condensation of homocysteine with serine, and glycine is produced by the transfer of side chain β-carbon atoms to tetrahydrofolate in a reaction catalyzed by serine transhydroxymethylase. Phenylalanine and tyrosine are synthesized from erythrose 4-phosphate and phosphoenolpyruvate, which are glycolysis and pentose phosphate pathway precursors in the nine-step biosynthetic pathway that differ only in the final two steps after the synthesis of the prephenate. Tryptophan is also synthesized from these two initial molecules, but its synthesis is an 11 step pathway. Tyrosine may also be synthesized from phenylalanine in a reaction catalyzed by phenylalanine hydroxylase. Alanine, valine and leucine are all biosynthetic products of pyruvate, the final product of glycolysis. Aspartate is formed from oxaloacetate, the intermediate of the citric acid cycle. Asparagine, methionine, threonine and lysine are each produced by the conversion of aspartate. Isoleucine is formed from threonine. Histidine is produced from 5-phosphoribosyl-1-pyrophosphate, an activated sugar in a complex nine-step pathway.

Amino acids in excess of the cell's protein synthesis requirements cannot be stored, but instead are degraded to serve as intermediates to the cell's major metabolic pathways [see, for example, Stryer, L. Biochemistry 3rd ed. Degradation and the Urea Cycle "p. 495-516 (1988)). Although cells can convert unnecessary amino acids into useful metabolic intermediates, amino acid production is a lossy synthesis given the energy, precursor molecules, and enzymes required to synthesize these amino acids. Thus, it is not surprising that the presence of a particular amino acid modulates the biosynthesis of the amino acid by feedback inhibition, which slows or completely stops its own production. Biochemistry 3rd ed. Ch. 21 "Amino Acid Degradation and the Urea Cycle" p. 495-516 (1988)). Thus, the yield of any particular amino acid is limited by the amount of amino acid present in the cell.

B. Metabolism and Uses of Vitamins, Cofactors and Nutritional Supplements

Vitamins, cofactors, and nutrients, although easily synthesized by other organisms such as bacteria, constitute another group of molecules that higher animals lose their ability to synthesize and need to consume. These molecules are themselves bioactive substances or precursors of biologically active substances that can act as electron carriers or intermediates in various metabolic pathways. In addition to their nutritional value, these compounds have considerable industrial value as pigments, antioxidants and catalysts, or as auxiliaries for other processes (for the structure, activity and industrial use of these compounds, see, for example, Ullman's Encyclopedia of Industrial). Chemistry, "Vitamins" vol. A27, p. 443-613, VCH: Weinheim, 1996)] The term "vitamin" is recognized in the art and is necessary for the normal functioning of an organism, but the organism synthesizes itself. The group of vitamins may also include cofactors and nutrient compounds The term "cofactor" includes nonproteinaceous compounds necessary for normal enzymatic activity to occur. It is preferred that the cofactor molecule of the invention is organic and the term "nutritive agent" is of health benefit to plants and animals, especially humans. It includes dietary supplements. Examples of such molecules are vitamins, antioxidants and some lipids (e.g., polyunsaturated fatty acids).

The biosynthesis of these molecules occurring in organisms capable of producing them, such as bacteria, is well characterized (Ullman's Encyclopedia of Industrial Chemistry, "Vitamins" vol. A27, p. 443-613, VCH: Weinheim, 1996; Michal , G. (1999) Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology, John Wiley &Sons; Ong, AS, Niki, E. & Packer, L. (1995) "Nutrition, Lipids, Health, and Disease" Proceedings of the UNESCO / Confederation of Scientific and Technological Associations in Malaysia, and the Society for Free Radical Research-Asia, held Sept. 1-3, 1994 at Penang, Malaysia, AOCS Press: Champaign, IL X, 374 S).

Thiamine (vitamin B ₁ ) is produced by chemical coupling of pyrimidine and thiazole residues. Riboflavin (vitamin B ₂ ) is synthesized from guanosine-5'-triphosphate (GTP) and ribose-5'-phosphate. Riboflavin is then used for the synthesis of flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD). Compound groups collectively referred to as "vitamin B ₆ " (eg, pyridoxine, pyridoxamine, pyridoxa-5'-phosphate and commercially available pyridoxine hydrochloride) are all typical structural units of 5-hydroxy-6 -A derivative of methylpyridine. Pantothenate (pantothenic acid, (R)-(+)-N- (2,4-dihydroxy-3,3-dimethyl-1-oxobutyl) -β-alanine) is produced by chemical synthesis or fermentation Can be. The final stage of biosynthesis of pantothenate consists of ATP-induced condensation of β-alanine and pantosan. Biosynthesis steps for converting to pantosan, biosynthesis steps for converting to β-alanine, and enzymes involved in condensation to pantothenic acid are known. The metabolic active form of pantothenate is coenzyme A, the biosynthesis for coenzyme A proceeds in a five step enzymatic reaction. Pantothenate, pyridoxal-5'-phosphate, cysteine and ATP are precursors of coenzyme A. These enzymes not only catalyze the formation of pantothenate, but also (R) -pantosan, (R) -pantolactone, (R) -panthenol (provitamin B ₅ ), pantethene (and derivatives thereof) and coenzymes. The production of A also catalyzes.

Biosynthesis of biotin from pimeloyl-CoA, the precursor molecule in microorganisms, has been studied in detail and several related genes have been identified. A number of corresponding proteins have been found to be involved in Fe-cluster synthesis as well, and these proteins are members of nifS class proteins. Lipoic acid is derived from octanoic acid and acts as a coenzyme in energy metabolism, which is part of the pyruvate dehydrogenase conjugate and α-ketoglutarate dehydrogenase conjugate in energy metabolism. Folates are all a group of derivative materials of folic acid, and folic acid is derived from L-glutamic acid, p-amino-benzoic acid and 6-methylphtherine. Biosynthesis of folic acid and its derivatives, starting from the metabolic intermediate guanosine-5′-triphosphate (GTP), L-glutamic acid and p-amino-benzoic acid, has been studied in detail in some microorganisms.

Corinoids (eg cobalamin and especially vitamin B ₁₂ ) and porphyrins belong to the group of chemicals characterized by the tetrapyrrole ring system. The biosynthesis of vitamin B ₁₂ is sufficiently complex in that many enzymes and substrates are now known, although their characteristics are not fully characterized. Nicotinic acid (nicotinate) and nicotinamide are pyridine derivatives, also referred to as "niacin". Niacin is an important coenzyme NAD (nicotinamide adenine dinucleotide), NADP (nicotinamide adenine dinucleotide phosphate) and their reduced precursors.

Mass production of these compounds relies mainly on cell-free chemical synthesis, although some of these chemicals, such as riboflavin, vitamin B ₆ , pantothenate and biotin, are also produced by mass culture of microorganisms. Only vitamin B ₁₂ is produced entirely by fermentation due to the complexity of the synthesis of vitamin B ₁₂ . In vitro methodologies require significant inputs of material and time, often of high cost.

C. Metabolism and Uses of Purines, Pyrimidines, Nucleosides, and Nucleotides

Purine and pyrimidine metabolic genes and their corresponding proteins are important targets for the treatment of tumor diseases and viral infections. The term "purine" or "pyrimidine" includes nitrogen containing bases that are components of nucleic acids, coenzymes and nucleotides. The term “nucleotide” includes the basic structural unit of a nucleic acid molecule, consisting of a nitrogen containing base, an pentose sugar (for RNA, the sugar is ribose; for DNA, the sugar is D-deoxyribose) and phosphoric acid. do. The term “nucleotide” includes molecules that act as precursors of a nucleotide but are free of phosphate residues possessed by the nucleotide. By inhibiting the biosynthesis of these molecules or inhibiting the recruitment of these molecules to form nucleic acid molecules, it is possible to inhibit RNA and DNA synthesis; By inhibiting this activity in a manner that targets cancer cells, the ability to divide and replicate tumor cells can be suppressed. There are also nucleotides that act as energy stores or coenzymes (ie, FAD and NAD) rather than forming nucleic acid molecules.

It has been described in the literature that these chemicals can be used in these medical conditions by influencing purine and / or pyrimidine metabolism (eg, Christopherson, RI and Lyons, SD (1990) "Potent inhibitors of de novo pyrimidine and purine biosynthesis as chemotherapeutic agents. "Med. Res. Reviews 10: 505-548). The study of enzymes involved in purine and pyrimidine metabolism has focused on the development of new drugs that can be used, for example, as immunosuppressive or antiproliferative agents (Smith, JL, (1995) "Enzymes in nucleotide synthesis." Curr. Opin.Struct. Biol. 5: 752-757; (1995) Biochem Soc. Transact. 23: 877-902). However, purine and pyrimidine bases, nucleosides and nucleotides are used as intermediates in the biosynthesis of various fine chemicals (e.g., thiamin, S-adenosyl-methionine, folate or riboflavin), energy for cells For use as a carrier (e.g., ATP or GTP), and for the chemical itself, which is usually used as a flavor enhancer (e.g., IMP or GMP) or for various medical uses [e.g. , Kuninaka, A. (1996) Nucleotides and Related Compounds in Biotechnology vol. 6, Rehm et al., Eds. VCH: Weinheim, p. 561612. In addition, there is an increasing number of studies to develop crop protection chemicals, including fungicides, herbicides and pesticides, by targeting enzymes involved in purine, pyrimidine, nucleoside or nucleotide metabolism.

The metabolism of these compounds in bacteria has been characterized (see, for example, Zalkin, H. and Dixon, JE (1992) "de novo purine nucleotide biosynthesis", in: Progress in Nucleic Acid Research and Molecular Biology, vol. 42, Academic Press :, p. 259-287; and Michal, G. (1999) "Nucleotides and Nucleosides", Chapter 8 in: Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology, Wiley: New York )). Purine metabolism is the subject of intensive research and is essential for the normal functioning of cells. Impaired purine metabolism in higher animals can lead to serious diseases such as gout. Purine nucleotides are subjected to ribose-5- in a series of steps through inosine-5'-phosphate (IMP), an intermediate compound that produces guanosine-5'-monophosphate (GMP) or adenosine-5'-monophosphate (AMP). Triphosphates that are synthesized from phosphates and used as nucleotides from these GMPs or AMPs are readily formed. Since these compounds are also used as energy stores, their degradation provides energy for many different biosynthetic processes in the cell. Pyrimidine biosynthesis proceeds by the formation of uridine-5'-monophosphate (UMP) from ribose-5-phosphate. UMP is then converted to cytidine-5'-triphosphate (CTP). The deoxy form of all these nucleotides is produced in a one step reduction reaction in which the diphosphate ribose form of nucleotide is converted to the diphosphate deoxyribose form of nucleotide. Upon phosphorylation, these molecules can participate in DNA synthesis.

D. Trehalose  Metabolism and Uses

Trehalose consists of two sugar molecules bound by α, α-1,1 bonds. Trehalose is commonly used in the food industry and the beverage industry as an additive for sweeteners, dried or frozen foods. However, it is also used in the pharmaceutical, cosmetic and biotechnology industries. See, eg, Nishimoto et al., (1998) US Patent No. 5,759,610; Singer, MA and Lindquist, S. (1998) Trends Biotech. 16: 460467. Paiva, CLA and Panek, AD (1996) Biotech. Ann. Rev. 2: 293-314; and Shiosaka, M. (1997) J. Japan 172: 97-10). Trehalose is produced by enzymes from a number of microorganisms and naturally released into the surrounding medium, which can be obtained from the medium using methods known in the art.

II . Resistance to damage from chemicals, environmental stress and antibiotics

Fine chemicals are typically produced by large-scale cultivation of bacteria developed to produce and secrete these molecules in large quantities. However, this type of large scale fermentation exposes the microorganisms to various types of stress. These stresses include environmental stresses and chemical stresses.

A. Resistance to Environmental Stress

Typical examples of environmental stresses encountered in large-scale fermentation cultures are mechanical stress, thermal stress, limited oxygen stress, oxygen radical stress, pH stress and osmotic stress. The stirring device used in most large scale fermenters to heat the culture to ensure the aeration of the culture raises the temperature of the culture. The rise in temperature leads to a well-characterized heat-shock response, in which a set of proteins that not only aids the survival of bacteria exposed to high temperatures but also increases survival in response to a number of other environmental stresses. Expressed in shock response (Neidhardt, FC, et al., Eds. (1996) E coli and Salmonella. ASM Press: Washington, DC, p. 1382-1399; Wosten, MM (1998) FEMS Microbiology Reviews 22 ( 3): 127-50; Bahl, H. et al. (1995) FEMS Microbiology Reviews 17 (3): 341-348; Zimmerman, JL, Cohill, PR (1991) New Biologist 3 (7): 641-650; Samali, A., and Orrenius, S. (1998) Cell.Stress Chaperones 3 (4): 228-236, the contents of which are incorporated herein by reference). The heat-shock response of bacteria is regulated by specific sigma factors and other cellular regulators of gene expression (Hecker, M., Volker, U (1998). Molecular Microbiology 29 (5): 1129-1136). One of the biggest problems that come across when cells are exposed to high temperatures is that protein folding is impaired. New proteins have sufficient kinetic energy in high temperature environments where it is difficult to exist with a stable structural length sufficient to allow the growing polypeptide chains to be properly folded. Has Thus, two of the key types of proteins expressed during a heat-shock reaction are chaperones (proteins that help fold or unfold other proteins, for example Fink, A. L. (1999) Physiol. Rev.

79 (2): 425-449), and proteases capable of digesting any protein that is improperly folded. Examples of chaperones expressed during the heat-shock reaction are GroEL and DNAK, and proteases known to be expressed during this cellular response to heat-shock are Lon, FtsH and CIpB.

Environmental stresses other than heat can also trigger stress responses. Although the fermenter agitation process implies the introduction of oxygen into the culture, the supply of oxygen is in a limited state, especially when the growth of the culture proceeds and the oxygen demand of the culture increases, inadequate supply of oxygen to the microorganisms. Another stress. In addition, cells in fermentor cultures are exposed to a number of osmotic stresses, especially when nutrients are added to the culture resulting in extracellular and intracellular low concentrations of these nutrient molecules. Moreover, the large amount of desired molecules produced by these organisms in culture can contribute to the osmotic stress of the bacteria. Finally, aerobic mechanisms such as those used by Corynebacterium glutamicum produce waste carbon dioxide, which is converted to carboxylic acids so that the culture medium can be acidified when the molecules are secreted. Thus, bacteria in culture are often exposed to acidic pH stress. Conversely, when high concentrations of basic waste molecules such as ammonia are present in the culture medium, bacteria in the culture may be exposed to basic pH stress.

To counter this environmental stress, bacteria have a good genetic system that is expressed upon exposure to one or more stresses, such as the heat-shock system. Genes expressed in response to osmotic stress encode proteins that can deliver or synthesize compatible solutes, for example, so that the osmotic influx or excretion of a particular molecule is slowed down to a manageable level. Other examples of stress-induced bacterial proteins are proteins involved in trehalose biosynthesis, proteins encoding enzymes involved in ppGpp metabolism, proteins involved in signal transduction, particularly proteins encoding two-component systems sensitive to osmotic pressure, and various stresses Proteins encoding transcription factors that respond to factors (eg, RssB homologues and / or sigma factors). Many of these genes and their protein products are known in the art.

B. Resistance to Chemical Stress

In addition to environmental stresses, cells may also experience a number of chemical stresses. These stresses can be divided into two types. The first kind is a natural waste of metabolism and other cellular functions, secreted by the cells into the surrounding medium. The second class is chemicals present in extracellular medium that do not originate from cells. Generally, when cells release concentrated intracellular cytotoxic toxic waste into a relatively much thinner extracellular medium, these wastes are dispersed such that the extracellular concentrations of compounds that are likely to be toxic are very low. However, this may not be the case in large-scale fermentation cultures of bacteria, as many bacteria grow in relatively small environments at high metabolic rates such that waste can accumulate in the medium to near toxic levels. Examples of such wastes are carbon dioxide, metal ions, and reactive oxygen species such as hydrogen peroxide. These compounds can interfere with the activity or structure of cell surface molecules, or enter the cells again and cause similar serious damage to proteins and nucleic acids. Some other chemicals that are harmful to the normal function of the cell can of course be found in extracellular medium. For example, metal ions such as mercury, cardium, nickel or copper are often found in sources of water and can form tight conjugates with cellular enzymes that interfere with the normal functioning of these proteins.

C. Resistance to Antibiotics

Antimicrobial proteins or antibiotics may be found in extracellular media through the involvement of a researcher, or as a natural product of another organism used to gain competitive advantage. Microbes have several mechanisms known in the art to protect themselves against antimicrobial chemicals. Degradation, modification and release of compounds that are toxic to cells is a common way microorganisms remove or detoxify antibiotics. Cytoplasmic “spill-pump” is known in many prokaryotic cells and shows similarities to the so-called “multidrug resistant” protein produced from higher eukaryotes (Neyfakh, AA, et al. (1991) Proc. Natl. Acad. Sci USA 88: 4781-4785. Examples of such proteins are E. coli. EmrAB from E. coli (Lomovskaya, O. and K. Lewis (1992) Proc. Natl. Acad. Sci. USA 89: 8938-8942), lmrB from B. subtilis (B. subtilis) Kumano, M. et al. (1997) Microbiology 143: 2775-2782). Smr from S. aureus (Grinius, LG et al. (1992) Plasmid 27: 119-129) or cmr from Corynebacterium glutamicum (Kaidoh, K. et al. (1997) Micro Drug Drug Resist. 3: 345-350. Corynebacterium glutamicum itself is not a pathogenic microorganism, in contrast to many other members of the genus Corynebacterium, such as Corynebacterium diphtheria or Corynebacterium pseudotubercosis. Several pathogenic Corynebacteriums, such as Corynebacterium jacaium and Corynebacterium ureaticum, are known to have multiple resistance to various antibiotics (Soriano, F. et al. (1995) Antimicrob. Agents Chemother. 39: 208-214.

Lincosamide is known as an effective antibiotic against Corynebacterium species (Soriano, F. et al. (1995) Antimicrob. Agents Chemother. 39: 208-214). An unexpected result of the present invention was the identification of genes encoding lincosamide-resistant proteins (particularly lincomycin-resistant proteins). The LMRB protein of Corynebacterium glutamicum is standard parameter (pairwise alignment parameter: slow / accumulate alignment: gap open penalty = 10.00, gap extension penalty = 0.10, protein weight matrix = BLOSUM 30, DNA weight matrix = IUB, Fast / Proxymate Alignment: Gap Penalty = 3, K-Tuple (Word) Size = 1, Top Diagonal Number = 5, Window Size = 5, Toggle Slow / Fast Pairwise Alignment = Slow Multiple Alignment Parameters: Gap Program CLUSTALW using opening penalty = 10.00, gap extension penalty = 0.05, delay divergence sequence = 40%, DNA transition weight = 0.50, protein weight matrix = BLOSUM series, DNA weight matrix = IUB, negative matrix = off) Using version 1.7 of (Thompson, JD, Higgins, DG, Gibson, TJ (1994) Nucl.Acids Res. 22: 4673-4680) As sanhan, it shows a product with 40% homology of lmrB gene from Bacillus subtilis (also refer to the binary bank approval claim No. AL009126).

Environmental stresses, chemical stresses, and antibiotic or other antimicrobial stresses can affect the behavior of microorganisms during fermentation culture and can affect the production of desired compounds from these organisms. For example, the osmotic stress of a microorganism can cause inadequate or improperly fast ingestion of one or more compounds that can ultimately result in cell damage or cell death due to osmotic shock. Similarly, chemicals that are added externally and present in the culture (eg, antimicrobial compounds added with the intention of removing unwanted microorganisms) or chemicals that the bacteria themselves produce and present in the culture (eg, Waste compounds such as heavy metals or oxygen radicals or even antimicrobial compounds) may inhibit the production of fine chemicals or even cause the death of an organism. The genes of the present invention specifically encode the Corynebacterium glutamicum protein, which acts against the cause or effect of environmental or chemical stress to prevent cell damage or death.

III . Elements and Methods of the Invention

The present invention is based, in part, on the discovery of novel nucleic acid molecules called SRT nucleic acid and protein molecules that increase the viability of Corynebacterium glutamicum under chemically or environmentally harmful conditions. In one embodiment, the SRT molecule functions to confer Corynebacterium glutamicum resistance to one or more environmental or chemical stresses. In a preferred embodiment, the activity of the SRT molecules of the invention affects the production of the desired fine chemicals by this organism. In a particularly preferred embodiment, the activity of the SRT molecules of the invention is modulated to control the yield, productivity and / or production efficiency of one or more fine chemicals from Corynebacterium glutamicum.

The term "SRT protein" or "SRT polypeptide" includes proteins involved in the resistance of Corynebacterium glutamicum to one or more environmental or chemical stresses. Examples of SRT proteins include those encoded by the SRT genes listed in Table 1 and the sequences of odd sequence numbers. The term "SRT gene" or "SRT nucleic acid sequence" includes nucleic acid sequences encoding SRT proteins, consisting of coding regions and corresponding untranslated 5 'and 3' sequence regions. Examples of SRT genes include those listed in Table 1. The term “production” or “productivity” is a term recognized in the art and includes the concentration of fermentation product (eg, kg product per liter per hour) formed within a given time and within a given fermentation volume. . The term "production efficiency" includes the time required to achieve a specific product concentration (eg, the time it takes for a cell to obtain a specific proportion of fine chemical product). The term "yield" or "product / carbon yield" is a term recognized in the art and includes the efficiency of conversion of carbon sources to products (ie, fine chemicals). This term is generally denoted, for example, in kg of product per kg of carbon source. By increasing the yield or production efficiency of the compound, the amount of recovered molecules or useful molecules of the compound in the obtained amount of culture obtained for a given time is increased. The term "biosynthesis" or "biosynthetic pathway" is a term recognized in the art and involves the synthesis of compounds, preferably organic compounds, from intermediate compounds by cells, and can be a multistep highly regulated process. The term "degradation" or "degradation pathway" is a term recognized in the art and includes the degradation by a cell into a degradation product of a compound, preferably an organic compound (generally, a molecule of smaller or less complex structure) and This can be a multi-step, highly controlled process. Subsequent metabolism of certain compounds (eg, metabolism of amino acids such as glycine) includes the overall biosynthesis, modification, and degradation pathways of cells associated with such compounds. The terms "resistance" and "resistant" are known in the art and include the ability of the cell to be unaffected by such stress upon exposure to chemical or environmental stresses that may adversely affect the normal functioning of the cell. . The term "stress" or "danger" includes factors that are detrimental to the normal function of the cell, such as Corynebacterium glutamicum. Examples of stress include "chemical stress" (where a cell is exposed to one or more chemicals that are harmful to the cell) and "environmental stress" (where the cell is exposed to environmental conditions other than those to which the cell has adapted). . Chemical stress is other chemicals that are environmentally responsible, including, but not limited to, reactive metabolic species or natural metabolic waste, such as, but not limited to, carbon dioxide, or heavy metal ions, such as antibiotics, or bactericidal proteins. Can be. Environmental stresses can be, for example, but not limited to temperatures outside the normal range, oxygen availability below optimal levels, osmotic pressure or extreme pH.

In another embodiment, the SRT molecules of the present invention can regulate the production of desired molecules such as fine chemicals in microorganisms such as Corynebacterium glutamicum. Using recombinant genetic engineering techniques, the SRT protein can be engineered to modulate the function of one or more SRT proteins of the invention. To increase the cell's resistance to one or more stresses, active variations in the stress response, resistance or resistance inheritance can improve cell growth and proliferative capacity under relatively high stress fermentation culture conditions. For example, by overexpressing or genetically engineering the chaperone molecule to optimize the activity of the heat-shock induced chaperone molecule, the ability of the bacteria to accurately fold the protein under abnormal temperature conditions can be increased. Low amounts of misfolded (and amido misregulated or nonfunctional) proteins increase the ability of cells to function normally under these culture conditions, thereby increasing cell viability. This overall increase in the number of cells in the culture, with higher viability and activity, also increases the yield, productivity, and / or production efficiency of one or more desired fine chemicals, which are cultures that produce at least these chemicals. This is because the number of cells in the water is relatively higher.

Isolated nucleic acid sequences of the present invention are contained within the genome of Corynebacterium glutamicum strains that are available from the American Type Culture Collection, designated ATCC13032. The nucleotide sequence of isolated Corynebacterium glutamicum SRT DNA and the expected amino acid sequence of Corynebacterium glutamicum SRT protein are shown in the sequence listing as odd sequence numbers and even sequence numbers, respectively.

Computer analysis was performed to classify and / or identify these nucleotide sequences as sequences encoding chemical and environmental stress resistant and resistant proteins.

The invention also relates to a protein having an amino acid sequence that is substantially homologous to an amino acid sequence of the invention (eg, a sequence of even sequence numbers in the sequence listing). As used herein, a protein having an amino acid sequence that is substantially homologous to the selected amino acid sequence is at least about 50% homologous to the selected amino acid, eg, the entire amino acid sequence selected. A protein having an amino acid sequence that is substantially homologous to the amino acid sequence selected is about 50 to 60% or more, preferably about 60 to 70% or more, more preferably about 70 to 80%, 80 to 80, based on the amino acid sequence selected. 90%, or at least 90-95%, most preferably at least about 96%, at least 97%, at least 98%, at least 99% homology. Ranges and confirmation values (for example, 75% -80% identical, 85-87% identical, 91-92% identical) corresponding to the middle of the above mentioned values are also considered in the present invention. For example, it is intended to include a range of confirmation values using a combination of any of the above values, referred to as upper and / or lower limits.

The SRT protein or biologically active portion or fragment thereof of the present invention may confer resistance or resistance to one or more chemical or environmental stresses, or may have one or more of the activities listed in Table 1.

Various aspects of the invention are described in more detail in the following subsections.

A. Isolated Nucleic Acid Molecules

One aspect of the invention provides for use as hybridization probes or primers for identifying or amplifying SRT-encoding nucleic acids (eg, SRT DNA), as well as isolated nucleic acid molecules encoding SRT polypeptides or biologically active portions thereof. Enough nucleic acid fragments. As used herein, the term “nucleic acid molecule” includes DNA or RNA analogs generated using DNA molecules (eg cDNA or genomic DNA), RNA molecules (eg mRNA), and nucleotide analogues. I mean. The term also includes untranslated sequences located at both the 3 'and 5' ends of the coding region of the gene, at least about 100 nucleotide sequences upstream from the 5 'end of the coding region, and 3' of the coding region of the gene. At least about 20 nucleotide sequences downstream from the terminus. The nucleic acid molecule may be single stranded or double stranded, preferably double stranded DNA. An “isolated” nucleic acid molecule is one that is separated from other nucleic acid molecules present in a natural source of nucleic acid. Preferably, an “isolated” nucleic acid does not have a sequence that is native to both sides of the nucleic acid (ie, sequences located at the 5 ′ and 3 ′ ends of the nucleic acid) in the genomic DNA of the organism from which it is derived. For example, in various embodiments, an isolated SRT nucleic acid molecule is about 5 kb on both sides of the original nucleic acid molecule in the genomic DNA of the cell from which it is derived (eg, Corynebacterium glutamicum cells), Nucleotide sequences of size less than 4 kb, 3 kb, 2 kb, 1 kb, 0.5 kb or 0.1 kb. Moreover, an “isolated” nucleic acid molecule, eg, a DNA molecule, does not contain substantially any other cellular material or culture medium when produced by recombinant technology, or a chemical precursor or other chemical when chemically synthesized. Do not.

Standard molecular biology techniques and the sequence information provided herein can be used to isolate nucleic acid molecules of the invention, eg, nucleic acid molecules having nucleotide sequences of odd sequence numbers in the sequence listing, or portions thereof. For example, all or part of one of the sequences of odd sequence numbers in the sequence listing can be used as hybridization probes and standard hybridization techniques (eg, Sambrook, J., Fritsh, EF, and Maniatis T. Molecular Cloning: A Laboratory Manual.2nd, ed., Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989), using Corynebacterium from Corynebacterium glutamicum libraries using a hybridization technique. Leeum glutamicum SRT DNA can be isolated. Furthermore, nucleic acid sequences of the invention (e.g., sequences of odd sequence numbers in the sequence listing) by polymerase chain reaction using oligonucleotide primers designed based on the same sequences Nucleic acid sequences of the invention (e.g., sequences of odd sequence numbers) by polymerase chain reaction using oligonucleotide primers designed on the basis of Nucleic acid molecules comprising all or part of one of For example, mRNA can be isolated from normal endothelial cells (eg, by guanidinium-thiocyanate extraction method of Chilgwin et al. (1979) Biochemistry 18: 5294-5299), and DNA Is a reverse transcriptase (eg, Moloney MLV reverse transcriptase available from Gibco / BRL (Bethesda, MD); or from Seikagaku America, Inc., St. Petersburg, FL) Available AMV reverse transcriptase). Synthetic oligonucleotide primers can be designed for amplifying polymerase chain reactions based on one of the nucleotide sequences listed in the sequence listing. Nucleic acids of the invention can be amplified using cDNA or, alternatively, genomic DNA as a template and using appropriate oligonucleotide primers according to standard PCR amplification techniques. The nucleic acid thus amplified can be cloned into an appropriate vector and characterized by DNA sequencing. In addition, oligonucleotides corresponding to SRT nucleotide sequences can be prepared using standard synthetic techniques, eg, automated DNA synthesizers.

In a preferred embodiment, the isolated nucleic acid molecules of the invention comprise one of the nucleotide sequences described in the sequence listing. Nucleic acid sequences of the invention as described in the Sequence Listing correspond to Corynebacterium glutamicum SRT DNA of the invention. This DNA contains the 5 'untranslated and 3' untranslated sequences as shown in the sequences of each odd sequence number in the sequence listing, as well as the sequences encoding the SRT proteins (ie, the "coding regions" shown in the sequence of odd sequence numbers in the sequence listing). It also includes sequences. Alternatively, the nucleic acid molecule may comprise only the coding region of one of the nucleic acid sequences of the sequence listing.

For the purposes of the present application, each of the nucleic acid and amino acid sequences set forth in the sequence listing is a RXA, RXN or RXS number for identification with five numbers following the name "RXA", "RXN" or "RXS" (ie, RXA01524, It will be understood to have RXN00493 or RXS01027). Each nucleic acid sequence consists of three parts, the 5 'upstream region, the coding region and the downstream region. To avoid confusion, each of these three regions is identified by the same RXA, RXN or RXS designation. In addition, “one of the odd sequences in the sequence listing” refers to any of the nucleic acid sequences in the sequence listing and may also be distinguished by their other RXA, RXN or RXS names. The coding region of each of these sequences is also translated into the corresponding amino acid which is described by the even sequence number in the sequence listing (the corresponding nucleic acid sequence is described below). For example, the coding region for RXA01524 is set forth in SEQ ID NO: 1 and the amino acid sequence it encodes is set forth in SEQ ID NO: 2. The sequences of the nucleic acid molecules of the invention are identified by the same RXA, RXN or RXS name as the amino acid molecule they encode so that they can be easily interrelated. For example, the amino acid sequence referred to as RXA01524 is a translation of the coding region of the nucleotide sequence of the nucleic acid molecule RXA01524, and the amino acid sequence referred to as RXN00034 is a translation of the coding region of the nucleotide sequence of the nucleic acid molecule RXN00034 and the amino acid sequence referred to as RXN00568 Is a translation of the coding region of the nucleotide sequence of the nucleic acid molecule RXN00568. The correspondence between the RXA, RXN and RXS nucleotide and amino acid sequences of the present invention and their assigned sequence numbers is set forth in Table 1.

Some of the genes of the invention are "F-labeled genes". F-labeled genes include the genes listed in Table 1, which have 'F' before the RXA, RXN or RXS name. For example, as shown in Table 1, SEQ ID NO: 7, referred to as "F RXA00498," is an F-labeled gene, and SEQ ID NO: 25, 33, and 37 ("F RXA01345", "F RXA02543" and "F in Table 1, respectively). The same applies to RXA02282 ".

In one embodiment, the nucleic acid molecules of the invention do not include those described in Table 2. For the dapD gene, the sequence for this gene is described in Wehrmann, A., et al, (1998) J. Bacteriol. 180 (12): 3159-3165. However, the sequences obtained by the inventors are considerably longer than the published sequence forms. The published sequence form is dependent on inaccurate start codons and therefore is believed to represent only a fragment of the actual coding region.

In another preferred embodiment, the isolated nucleic acid molecules of the invention comprise nucleic acid molecules that are complements of one of the nucleotide sequences of the invention (eg, sequences of odd sequence numbers in the sequence listing). Nucleic acid molecules complementary to one of the nucleotide sequences shown in the present invention are sufficiently complementary to one of the nucleotide sequences shown in the sequence listing (e.g., odd sequence number sequences), so that Hybridization forms a stable double chain.

In another preferred embodiment, the isolated nucleic acid molecules of the invention comprise about 50%, 51%, 52%, 53% of the nucleotide sequences of the invention (eg, one of the sequences of odd sequence numbers in the sequence listing). , 54%, 55%, 56%, 57%, 58%, 59% or 60% or more, preferably about 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68% , 69%, or 70% or more, more preferably about 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, or 80%, 81%, 82% , 83%, 84%, 85%, 86%, 87%, 88%, 89%, or 90%, or 91%, 92%, 93%, 94% or more, even more preferably about 95%, 96 Nucleotide sequences having at least%, 97%, 98%, or 99% homology, or portions thereof. Moderate ranges and identity values (eg, 70-90% identical or 80-95% identical) for the aforementioned ranges are included in the present invention. For example, the range of identity values using any combination of the above mentioned upper and / or lower limits is included in the present invention. In a further preferred embodiment, the isolated nucleic acid molecules of the invention comprise nucleotide sequences that hybridize with one or a portion of the nucleotide sequences of the invention, for example under stringent conditions.

In addition, the nucleic acid molecules of the present invention encode only a portion of the coding region of one of the sequences of odd sequence numbers in the sequence listing, for example, a biologically active portion of a fragment or SRT protein that can be used as a probe or primer. May include fragments. Identify and / or identify SRT homologues from other Corynebacteria or related species as well as SRT homologs in other types of cells and organisms using nucleotide sequences determined from cloning of the SRT gene from Corynebacterium glutamicum Probes and primers designed for use in cloning can be generated. Probes / primers typically comprise substantially purified oligonucleotides. Oligonucleotides are typically, under stringent conditions, the sense strand of one of the nucleotide sequences of the invention (eg, one of the sequences of odd sequence numbers in the sequence listing), the anti-sense sequence of one of these sequences, or naturally occurring Nucleotide sequence regions that hybridize with about 12, preferably about 25, more preferably about 40, 50 or 75 or more consecutive nucleotides of the variants thereof. Primers based on the nucleotide sequences of the invention can be used in PCR reactions to clone SRT homologues. Probes based on SRT nucleotide sequences can be used to find transcript or genomic sequences encoding proteins that are identical or homologous. In a preferred embodiment, the probe further comprises a label group attached thereto, for example, the label group can be a radioisotope, fluorescent compound, enzyme or enzyme cofactor. Such probes can be used to detect cells that misexpress SRT proteins, for example by measuring SRT-encoding nucleic acid levels in a cell sample, for example by detecting SRT mRNA levels or determining whether genomic SRT genes have been mutated or deleted. It can be used as part of a diagnostic test kit for identification.

In one embodiment, a nucleic acid molecule of the invention encodes a protein or portion thereof comprising an amino acid sequence that is sufficiently homologous to an amino acid sequence of the invention (eg, an even sequence number in the sequence listing), and The protein or portion thereof maintains the ability to confer resistance or resistance of Corynebacterium glutamicum to one or more chemical or environmental stresses. As used herein, the term “sufficient homologous” means the same or equivalent minimum number of amino acid residues (eg, one amino acid residue in one of the even numbered sequences in the sequence listing) to the amino acid sequence of the present invention. Refers to a protein or part thereof that may be involved in the resistance or resistance of Corynebacterium glutamicum to one or more chemical or environmental stresses, including amino acid sequences comprising amino acid residues having side chains similar to the above). Protein members of these metabolic pathways, as described herein, function to increase the resistance or resistance of Corynebacterium glutamicum to one or more environmental or chemical risks or stresses. Thus, "function of the SRT protein" contributes to the overall resistance of Corynebacterium glutamicum to elements in the periphery of the microorganism that may interfere with normal growth and or function of Corynebacterium glutamicum ( Or, directly or indirectly, in the yield, productivity and / or production efficiency of one or more fine chemicals. Examples of SRT protein activity are listed in Table 1.

In another embodiment, the protein is about 50% to 60% or more, preferably about 60% to 70% or more, and more preferably, the entire amino acid sequence of the invention (eg, the sequence of even sequence numbers in the sequence listing) Preferably about 70% to 80%, 80% to 90%, 90% to 95% or more, most preferably about 96%, 97%, 98%, 99% or more homologous. For the values mentioned above, the median of the range and homology (eg 75% -80% homology, 85% -87% homology, 91-92% homology) is also intended to be included in the present invention. For example, ranges of homology using a combination of any of the above mentioned values as upper and / or lower limits are intended to be encompassed.

Some of the proteins encoded by the SRT nucleic acid molecules of the invention are preferably biologically active portions of one of the SRT proteins. As used herein, the term "biologically active portion of an SRT protein" may confer resistance or resistance to one or more environmental or chemical stresses or risks, or have an activity as described in Table 1 Part of a protein, eg, its domains / motifs. To determine if an SRT protein or a biologically active portion thereof can increase the resistance or resistance of Corynebacterium glutamicum to one or more environmental or chemical stresses or risks, an analysis of enzymatic activity will be performed. Can be. Such assay methods are well known to those skilled in the art, as described in Example 8.

Other nucleic acid fragments encoding biologically active portions of the SRT protein may comprise amino acid sequences of the invention (eg, by sequence expression) that express the coding portion of the SRT protein or peptide (eg, by recombinant expression in vitro). A portion of one of the even sequence numbers) can be isolated and evaluated by assessing the activity of the encoded portion of the SRT protein or peptide.

The present invention relates to an SRT protein which differs from one of the nucleotide sequences shown in the present invention (e.g., the sequence of odd sequence numbers in the sequence listing) but is identical to the one encoded by the nucleotide sequence of the present invention due to the degeneracy of the genetic code. Nucleic acid molecules (and fragments thereof) that encode. In another embodiment, an isolated nucleic acid molecule of the invention has a nucleotide sequence encoding a protein having an amino acid sequence shown in the sequence listing (eg, an even sequence number). In another embodiment, the nucleic acid molecules of the invention are full-length Corynebacterium glutamicum that are substantially homologous to the amino acid sequences of the invention (encoded by the open reading frame shown in the sequence of odd sequence numbers in the sequence listing). Code the protein.

Those skilled in the art will appreciate that in one embodiment the sequences of the present invention do not comprise prior art sequences such as the Genbank sequences described in Table 2 or 4, which were previously available for the present invention. In one embodiment, the invention is characterized by the identity of a nucleotide or amino acid sequence of the invention that is greater than the prior art sequences (e.g., the Genbank sequence described in Tables 2 or 4 (or proteins encoded by such sequences)). Nucleotide sequence and amino acid sequence). For example, the present invention is larger (or greater) than the nucleotide sequence referred to as RXA00084 (SEQ ID NO: 189) and greater than (greater than) the nucleotide sequence referred to as RXA00605 (SEQ ID NO: 11) Or) a nucleotide sequence having at least 56% identity therewith and a nucleotide sequence that is larger (or larger) than at least 50% identity with the nucleotide sequence referred to as RXA00886 (SEQ ID NO: 39). Those skilled in the art will examine the GAP-calculated percent identity shown in Table 4 for each of the three highest hits for a given sequence, and subtract 100 from the GAP-calculated highest percent identity in any of the inventions. The lower threshold of percent identity for a given sequence may be calculated. Those skilled in the art will also appreciate the percent identity greater than this calculated lower threshold (eg, about 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%). Or at least 60%, preferably at least about 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, or 70%, more preferably about 71%, 72 %, 73%, 74%, 75%, 76%, 77%, 78%, 79%, or 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88% , 89%, or 90%, or 91%, 92%, 93%, 94% or more, even more preferably about 95%, 96%, 97%, 98%, or 99% identity) And amino acid sequences are included in the present invention.

Those skilled in the art will appreciate that in addition to the Corynebacterium glutamicum SRT nucleotide sequences described by odd sequence numbers in the sequence listing, polymorphisms in the DNA sequence that result in changes in the amino acid sequence of the SRT protein (eg, Corynebacterium glue). Will be present in the Tamicum population). This genetic polymorphism in the SRT gene may exist between individuals in the population due to natural changes. As used herein, the terms "gene" and "recombinant gene" refer to a nucleic acid molecule comprising an open reading frame encoding an SRT protein, preferably Corynebacterium glutamicum SRT protein. Such natural changes typically alter the nucleotide sequence of the SRT gene by 1-5%. Any and all such nucleotide changes of the SRT gene and the resulting polymorphisms of the SRT gene that are the result of natural changes and do not alter the functional activity of the SRT protein are within the scope of the present invention.

Natural variants and nucleic acid molecules corresponding to the non-Corynebacterium glutamicum homologues of the Corynebacterium glutamicum SRT DNA of the present invention are those of the Corynebacterium glutamicum SRT nucleic acids disclosed herein. Based on homology, it can be isolated using Corynebacterium glutamicum DNA or portions thereof as hybridization probes under stringent hybridization conditions according to standard hybridization techniques. Thus, in another embodiment, an isolated nucleic acid molecule of the present invention hybridizes with a nucleic acid molecule that is at least 15 nucleotides in length and comprises nucleotide sequences of odd sequence numbers in the sequence listing under stringent conditions. In other embodiments, the nucleic acid is at least 30, 50, 100, or 250 nucleotides in length. As used herein, the term “hybridizing under strict conditions” is meant to describe conditions for hybridization and washing, in which nucleotide sequences that are typically at least 60% homologous to each other remain hybridized with each other. Preferably, these conditions are those under which sequences that are typically at least about 65%, more preferably at least about 70% and even more preferably at least about 75% homologous to each other remain hybridized to each other. Such stringent conditions are well known to those skilled in the art and described in Current Protocols in Molecular Biology, John Wiley & Sons, N.Y. (1989), 6.3.1-6.3.6. A preferred non-limiting example for stringent hybridization conditions is one or more washes in 0.2 × SSC, 0.1% SDS at 50-65 ° C. after hybridization in 6 × sodium chloride / sodium citrate (SSC) at about 45 ° C. Preferably, the isolated nucleic acid molecules of the present invention that hybridize with the nucleotide sequences of the present invention under stringent conditions correspond to naturally occurring nucleic acid molecules. As used herein, “naturally occurring” nucleic acid molecule refers to an RNA or DNA molecule having a nucleotide sequence that exists in nature (eg, encodes a natural protein). In one embodiment, the nucleic acid encodes a native Corynebacterium glutamicum SRT protein.

In addition to naturally occurring variants of the SRT sequences that may be present in the population, those skilled in the art will also appreciate that variations in the nucleotide sequences of the present invention result in changes in the amino acid sequence of the encoded SRT protein, without altering the functionality of the SRT protein. It will be appreciated that it can be introduced. For example, nucleotide substitutions that result in amino acid substitutions at “non-essential” amino acid residues can occur in the nucleotide sequences of the invention. “Non-essential amino acid” residues are residues that can be changed from the wild-type sequence of one of the SRT proteins (eg, the sequence of even sequence numbers in the sequence listing) without changing the activity of the SRT protein. Residues are required for SRT protein activity. However, other amino acid residues (eg, residues that are not conservative or semiconservative in a domain having SRT activity) may not be necessary for activity and thus can be changed without altering SRT activity.

Accordingly, another aspect of the invention relates to nucleic acid molecules encoding SRT proteins that include changes in amino acid residues that are not essential for SRT activity. Such SRT proteins differ in amino acid sequence from sequences of even sequence numbers in the sequence listing and retain at least one of the SRT activities described herein. In one embodiment, an isolated nucleic acid molecule comprises an amino acid sequence that is at least about 50% homologous to an amino acid sequence of the invention and is resistant to or resistant to Corynebacterium glutamicum against one or more environmental or chemical stresses. Nucleotide sequences encoding proteins that can be increased or proteins having one or more activities described in Table 1. Preferably, the protein encoded by the nucleic acid molecule is at least about 50-60% of the amino acid sequence of one of the sequences of odd sequence numbers in the sequence listing, more preferably at least about 60-70% of one of these sequences And even more preferably about 70-80%, 80-90%, 90-95% or more for one of these sequences, most preferably about 96%, 97%, 98 for one of the amino acid sequences of the invention There is homology over% or 99%.

In order to determine the homology (%) of two amino acid sequences (eg, one of the amino acid sequences of the invention and variant forms thereof) or two nucleic acid sequences, the sequences are aligned for optimal comparison purposes (eg, Gaps can be introduced into the sequence of one protein or nucleic acid for optimal alignment with another protein or nucleic acid). Subsequently, amino acid residues or nucleotides corresponding to amino acid positions or nucleotide positions are compared. If a position in one sequence (eg one of the amino acid sequences of the invention) is occupied by the same amino acid residue or nucleotide as the corresponding position in another sequence (eg a variant form of an amino acid sequence), the molecule is Homologous at the position (ie, as used herein, "homology" of an amino acid or nucleic acid is equivalent to "identity" of the amino acid or nucleic acid). The% homology between two sequences is a function of the number of identical positions shared by the sequences (ie,% homology = number of identical positions / total number of positions × 100).

By introducing a substitution, addition or deletion of one or more nucleotides into a nucleotide sequence of the invention such that a substitution, addition or deletion of one or more amino acids is introduced into the protein to be encoded, the protein sequences of the invention (e.g., An isolated nucleic acid molecule encoding an SRT protein homologous to the sequence of even sequence numbers) can be generated. Mutations can be introduced into one of the nucleotide sequences of the invention by standard techniques such as site-directed mutagenesis and PCR-mediated mutagenesis. Preferably, conservative amino acid substitutions can be made at the expected one or more non-essential amino acid residues. "Conservative amino acid substitutions" refer to replacement of amino acid residues with amino acid residues having similar side chains. Groups of amino acid residues with similar side chains are defined in the art. These groups include amino acids with basic side chains (eg lysine, arginine, histidine), amino acids with acidic side chains (eg aspartic acid, glutamic acid), amino acids with uncharged polar side chains (eg glycine, Asparagine, glutamine, serine, threonine, tyrosine, cysteine, amino acids with nonpolar side chains (e.g. alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine, tryptophan), amino acids with beta-branched side chains (e.g. Such as threonine, valine, isoleucine) and amino acids with aromatic side chains (eg tyrosine, phenylalanine, tryptophan, histidine). Thus, non-essential amino acid residues expected in SRT proteins are preferably substituted with other amino acid residues from the same side chain group. Alternatively, in another embodiment, the mutations can be introduced randomly along all or part of the SRT coding sequence, eg, by saturation mutagenesis, to identify variants that retain SRT activity. Variants can be screened for SRT activity described herein. One nucleotide sequence of the odd sequence numbered sequences in the sequence listing can then be mutagenesis and then recombinantly expressed the encoded protein, eg, the activity of the protein can be determined using the assays described herein (execution). See example 8.)

  In addition to the nucleic acid molecules encoding the SRT proteins described above, another aspect of the invention relates to an isolated nucleic acid molecule that is antisense thereto. An “antisense” nucleic acid includes a nucleotide sequence that is complementary to a “sense” nucleic acid encoding a protein, eg, complementary to the coding strand of a double-stranded DNA molecule or complementary to the mRNA sequence. Thus, antisense nucleic acids can hydrogen bond with sense nucleic acids. Antisense nucleic acids may be complementary to the entire SRT coding strand or only a portion thereof. In one embodiment, the antisense nucleic acid molecule is antisense to the “coding region” of the coding strand of the nucleotide sequence encoding the SRT protein. The term “coding region” means a region of nucleotide sequence comprising a codon that is translated into an amino acid residue (eg, the entire coding region of SEQ ID NO: 120 (RXA00600) comprises nucleotides 1 to 1098). In another embodiment, the antisense nucleic acid molecule is antisense relative to the "non-coding region" of the coding strand of the nucleotide sequence encoding the SRT. The term "non-coding region" refers to 5 'and 3' sequences (i.e., also referred to as 5 'and 3' untranslated regions) on either side of a coding region that are not translated into amino acids.

The present invention is based on Watson and Crick's base pairing rules, based on the coding strand sequence encoding the SRT disclosed herein (e.g., the sequence is listed as an odd sequence number in the sequence listing). Antisense nucleic acids of can be designed. This antisense nucleic acid molecule may be complementary to the entire coding region of the SRT mRNA, but more preferably is an oligonucleotide that is antisense only in a portion of the coding or non-coding region of the SRT mRNA. For example, antisense oligonucleotides may be complementary to the region around the translation initiation site of the SRT mRNA. Antisense oligonucleotides are, for example, about 5, 10, 15, 20, 25, 30, 35, 40, 45 or 50 nucleotides in length. Antisense nucleic acids of the present invention can be constructed using chemical synthesis and enzymatic ligation reactions using methods known in the art. For example, using naturally occurring nucleotides or various modified nucleotides designed to increase the biological stability of the molecule or to increase the physical stability of the double-strand formed between antisense and sense nucleic acid (e.g., , Phosphorothioate derivatives and acridine substituted nucleotides may be used), antisense nucleic acids (eg, antisense oligonucleotides) may be chemically synthesized. Examples of modified nucleotides that can be used to generate antisense nucleic acids include 5-fluorouracil, 5-bromouracil, 5-chlorouracil, 5-iodouracil, hypoxanthine, xanthine, 4-acetylcytosine, 5- (Carboxyhydroxylmethyl) uracil, 5-carboxymethylaminomethyl-2-thiouridine, 5-carboxymethylaminomethyluracil, dihydrouracil, beta-D-galactosylquaoxin, inosine, N6-isopentenyladenin , 1-methylguanine, 1-methylinosine, 2,2-dimethylguanine, 2-methyladenine, 2-methylguanine, 3-methylcytosine, 5-methylcytosine, N6-adenine, 7-methylguanine, 5-methyl Aminomethyluracil, 5-methoxyaminomethyl-2-thiouracil, beta-D-mannosylquaosine, 5'-methoxycarboxymethyluracil, 5-methoxyuracil, 2-methylthio-N6-isopentenyl Adenine, uracil-5-oxyacetic acid (v), bibutoxosocin, pseudouracil, quaosine, 2-thiocytosine, 5-methyl-2-thiouracil, 2-thiouracil, 4-thiouracil, 5-methyluracil, uracil-5-oxyacetic acid, methyl ester, uracil-5-oxyacetic acid (v), 5-methyl-2-thiouracil, 3- (3-amino -3-N-2-carboxypropyl) uracil, (acp3) w and 2,6-diaminopurine. Alternatively, the antisense nucleic acid can be biologically purified using an expression vector in which the nucleic acid is subcloned in the antisense direction (ie, RNA transcribed from the inserted nucleic acid is in the antisense direction relative to the desired target nucleic acid, as further described in the subsection below). Can be generated.

Antisense nucleic acid molecules of the invention are typically administered to cells or by hybridizing with or binding to mRNA and / or genomic DNA of cells that encode SRT proteins, for example by inhibiting transcription and / or translation. And is produced in the reaction system to inhibit the expression of the protein. Hybridization may be by conventional nucleotide complementarity to form a stable double chain, or may be due to, for example, in the case of antisense nucleic acid molecules that bind to a DNA double strand via specific interactions in a double groove major groove. . An antisense molecule can be modified to specifically bind to a receptor or antigen expressed on a selected cell surface, for example by linking the antisense nucleic acid molecule to a peptide or antibody that binds to a receptor or antigen on the cell surface. The vectors described herein may also be used to deliver antisense nucleic acid molecules to cells. In order to achieve sufficient intracellular concentrations of antisense molecules, vector constructs in which the antisense nucleic acid molecules are under the control of strong prokaryotic, viral or eukaryotic promoters are preferred.

In another embodiment, the antisense nucleic acid molecule of the invention is an α-anomeric nucleic acid molecule. α-anomeric nucleic acid molecules form specific double-stranded hybrids with complementary RNAs, where, in contrast to conventional β-units, the strands are parallel to each other (Gaultier et al. (1987)). Nucleic Acids.Res. 15: 6625-6641). Antisense nucleic acid molecules include 2'-o-methylribonucleotides (Inoue et al. (1987) Nucleic Acids Res. 15: 6131-6148) or chimeric RNA-DNA analogs (Inoue et al. (1987) FEBS Lett. 215: 327 -330).

In another embodiment, the antisense nucleic acid molecule of the invention is a ribozyme. Ribozymes are catalytic RNA molecules with ribonuclease activity and are capable of cleaving single stranded nucleic acids such as mRNA having complementary regions thereto. Thus, using ribozymes (e.g., Hammerhead ribozymes (as described in Haselhoff and Gerlach (1988) Nature 334: 585-591)), catalytic SRT mRNA transcripts can be catalyzed. The cleavage of SRT mRNA can be suppressed by cleavage. Based on the nucleotide sequence of the SRT DNA disclosed herein (ie, SEQ ID NO: 119 (RXA00600)), one can design a ribozyme with specificity for an SRT-encoding nucleic acid. For example, derivatives of Tetrahymena L-19 IVS RNA can be made wherein the nucleotide sequence of the active site is complementary to the nucleotide sequence cleaved from the SRT-encoding mRNA [eg, Cech. US Pat. No. 4,987,071, et al. And US Pat. No. 5,116,742 to Cech et al. Alternatively, SRT mRNA can be used to screen catalytically active RNA with specific ribonuclease activity from pools of RNA molecules. See, eg, Bartel, D. and Szostak, JW (1993) Science 261: 1411. -1418].

Alternatively, expression of the SRT gene by targeting a nucleotide sequence complementary to the regulatory region (eg, SRT promoter and / or enhancer) of the SRT nucleotide sequence to form a triple helix structure that prevents transcription of the SRT gene in the target cell. Can be suppressed [Generally, Helene, C. (1991) Anticancer Drug Des. 6 (6): 569-84; Helene, C. et al. (1992) Ann.N.Y. Acad. Sci. 660: 27-36; and Maher, L.J. (1992) Bioassays 14 (12): 807-15].

B. Recombinant Expression Vectors and Host Cells

Another aspect of the invention relates to an expression vector containing a nucleic acid encoding a vector, preferably an SRT protein (or a portion thereof). As used herein, the term "vector" refers to a nucleic acid capable of transporting other nucleic acids linked thereto. In one type of vector, "plasmid" means a circular double-stranded DNA ring into which additional DNA fragments can be ligated. Another type of vector, a viral vector, can ligate additional DNA fragments into the viral genome. Certain vectors have the ability to autonomously replicate within the introduced host cell (eg, bacterial vectors with bacterial replication initiation points and episomal vectors in mammals). Other vectors (eg, mammalian non-episomal vectors) are inserted into the host genome upon introduction into the host cell, thereby replicating together along the host genome. In addition, certain vectors may direct the expression of genes to which they are operatively linked. Such vectors are referred to herein as "expression vectors." In general, expression vectors used in recombinant DNA techniques are usually in the form of plasmids. Since plasmids are the most commonly used vector form, "plasmid" and "vector" can be used interchangeably herein. The invention also includes other forms of expression vectors such as viral vectors that provide equivalent functions (eg, replication defective retroviruses, adenoviruses and adeno-associated viruses).

The recombinant expression vector of the present invention comprises the nucleic acid of the present invention in a form suitable for expression of the nucleic acid in a host cell, and the suitable form means that the recombinant expression vector is one or more regulatory sequences selected based on the host cell for expression (the nucleic acid to be expressed). Operably linked to a sequence). In a recombinant expression vector, "operably linked" means that the desired nucleotide sequence is linked to the regulatory sequence (s) such that it is expressible (e.g., in an in vitro transcription / translation system or when the vector is introduced into a host cell. In host cells). The term "regulatory sequence" is intended to include promoters, enhancers and other expression control elements (eg, polyadenylation signals). Such regulatory sequences are described, for example, in Goeddel; Gene Expression Technology : Methods in Enzymology 185, Academic Press, San Diego, CA (1990). Regulatory sequences include constitutive expression of nucleotide sequences within multiple host cell types and expression of nucleotide sequences only within specific host cells. Preferred regulatory sequences include, for example, cos-, tac-, trp-, tet-, trp-tet-, lpp-, lac-, lpp-lac-, lacI ^q- , T7-, T5 which are preferably used in bacteria. -Promoters such as T3-, gal-, trc-, ara-, SP6-, arny, SPO2, λ-P _R -or λP _L. Further regulatory sequences include, for example, promoters from yeast and fungi such as ADC1, MFα, AC, P-60, CYC1, GAPDH, TEF, rp28, ADH, CaMV / 35S, SSU, OCS, lib4, usp, STLS1 , Promoters from plants such as B33, nos or ubiquitin- or cessolin-promoter. Artificial promoters can also be used. Those skilled in the art will appreciate that expression vector design may vary depending on factors such as the choice of host cell to be transformed, the degree of expression of the protein of interest, and the like. The expression vector of the present invention can be introduced into a host cell to produce a protein or peptide, including fusion proteins or peptides encoded by the nucleic acids described herein (eg, SRT proteins, mutant forms of SRT proteins, fusion proteins, etc.). .

Recombinant expression vectors of the invention can be designed for SRT protein expression in prokaryotic or eukaryotic cells. For example, the SRT gene may include bacterial cells such as C. glutamicum, insect cells (using baculovirus expression vectors), yeast and other fungal cells (Romanos, MA et. al . (1992) "Foreign gene expression in yeast: a review", Yeast 8: 423-488; van den Hondel, CAMJJ et al . (1991) "Heterologous gene expression in filamentous fungi" in: More Gene Manipulations in Fungi, JW Bennet & LL Lasure, eds., P. 396-428: Academic Press: San Diego; and van den Hondel, CAMJJ & Punt, PJ (1991) "Gene transfer systems and vector development for filamentous fungi, in: Applied Molecular Genetics of Fungi, Peberdy, JF et al ., eds., p. 1-28, Cambridge University Press: Cambridge], algae and multicellular plant cells (Schmidt, R. and Willmitzer, L. (1988) High efficiency Agrobacterium tumefactiens -mediated transformation of Arabidopsis thaliana leaf and cotyledon explants " Plant Cell Rep:. 583-586] can be expressed in the reference) or mammalian cells.

Suitable host cells are described in Goeddel, Gene Expression Technology : Methods in Enzymology 185, Academic Press, San Diego, CA (1990). Alternatively, recombinant expression vectors can be transcribed and translated in vitro, for example using T7 promoter regulatory sequences and T7 polymerase.

Expression of proteins in prokaryotic cells is generally performed with constitutive or inducible promoter containing vectors that allow for the expression of fusion or non-fusion proteins. Fusion vectors add a number of amino acids to their encoded protein, generally at the amino terminus of the recombinant protein. Such fusion vectors are typically for three purposes: 1) to increase expression of the recombinant protein, 2) to increase the solubility of the recombinant protein, and 3) to facilitate the purification of the recombinant protein by acting as a ligand in affinity purification. Usually, in the fusion expression vector, a protein cleavage site is introduced at the junction of the fusion residue and the recombinant protein, so that it is possible to separate the recombinant protein from the fusion residue after purifying the fusion protein. Such enzymes and their cognate recognition sequences include coagulation factors Xa, thrombin and enterokinase.

Typical fusion expression vectors include pGEX (Pharmacia Biotech Inc; see Smith, DB and Johnson, KS (1988) Gene 67: 31-40), pMAL (New England Biolabs, Beverly, Mass.) And pRIT5 (Pharmacia, Piscataway, NJ), which fuse the gurutaion-S-transferase (GST), maltose E binding protein or protein A (protein A), respectively, to the target recombinant protein. In one embodiment, the coding sequence of the SRT protein was cloned into the pGEX expression vector to generate a fusion protein coding vector containing the GST-thrombin cleavage site-X protein at the N-terminus to the C-terminus. The fusion protein can be purified by affinity chromatography using gurutaion-agarose resin. Recombinant SRT proteins that are not fused to GST can be obtained by cleaving the fusion protein with thrombin.

Suitable inductive non-fusion e. Examples of E. coli expression vectors are pTrc (see Amann et al ., (1988) Gene 69: 301-315), pLG338, pACYC184, pBR322, pUC18, pUC19, pKC30, pRep4, pHS1, pHS2, pPLc236, pMBL24, pLG200, pUR290, pINIII113-B1, λgt11, pBdCl and pET 11d (Studier et al ., Gene Expression Technology : Methods in Enzymology 185, Academic Press, San Diego, California (1990) 60-89 and Pouwels et al ., eds. (1985) Cloning Vectors. Elsevier: New York IBSN 0 444 904018). The expression of the target gene from the pTrc is due to the RNA polymerase transcription of the host via the hybrid trp-lac fusion promoter. Expression of the target gene from the pET 11d vector is by transcription mediated by co-expressed viral RNA polymerase (T7 gn1) via the T7 gn 10-lac fusion promoter. This viral polymerase is supplied by host strain BL21 (DE3) or HMS174 (DE3) from a supernatant lambda probe containing the T7 gn1 gene under the transcriptional control of the lacUV 5 promoter. Suitable vectors can be selected for the transformation of various bacteria. For example, plasmids pIJ101, pIJ364, pIJ702 and pIJ361 are known to be useful for transforming the genus Streptomyces, while plasmids pUB110, pC194 or pBD214 are suitable for transforming Bacillus spp. Plasmids used to transfer genetic information into Corynebacterium include pHM1519, pBL1, pSA77 or pAJ667 (Pouwels et. al ., eds. (1985) Cloning Vectors. Elsevier: New York IBSN 0 444 904018).

As part of a strategy for maximizing expression of recombinant proteins, the expression of these proteins in host bacteria has reduced the ability to cleave the recombinant protein by proteolysis (Gottesman, S., Gene). Expression Technology : Methods in Enzymology 185, Academic Press, San Diego, California (1990) 119-128). Another strategy is to modify the nucleic acid sequence of the nucleic acid to be inserted into the expression vector so that the individual codons for each amino acid are the preferred codons for the bacteria selected for expression (eg, Corynebacterium glutamicum). (See Wada et al . (1992) Nucleic Acids Res . 20: 2111-2118). Such modification of nucleic acid sequences of the invention can be performed by standard DNA synthesis techniques.

In another embodiment, the SRT protein expression vector is a yeast expression vector. Yeast S. Examples of expression vectors of cerevisiae include pYepSec1 (Baldari, et. al ., (1987) Embo J. 6: 229-234), 2μ, pAG-1, Yep6, Yep13, pEMBLYe23, pMFa (see Kurjan and Herskowitz, (1982) Cell 30: 933-943), pJRY88 Schultz et al ., (1987) Gene 54: 113-123) and pYES2 (Invitrogen Corporation, San Diego, Calif.). Suitable vectors and methods of making them for other fungi, such as filamentous fungi, are described by van den Hondel, CAMJJ & Punt, PJ (1991) "Gene transfer systems and vector development for filamentous fungi, in: Applied Molecular Genetics of Fungi, JF Peberdy, et al ., eds., p. 1-28, Cambridge University Press: Cambridge] and Pouwels et al ., eds. (1985) Cloning Vectors. Elsevier: New York (IBSN 0 444 904018).

Alternatively, baculo virus expression vectors can be used to express SRT proteins of the invention in insect cells. Baculovirus vectors for protein expression in cultured insect cells (eg Sf 9 cells) include pAc-based (Smith et al. al . (1983) Mol . Cell Biol . 3: 2156-2165) and pVL systems (see Lucklow and Summers (1989) Virology 170: 31-39).

In another embodiment, the SRT protein of the invention can be expressed in plant cells from single cell plant cells (eg algae) or higher plants (eg seed plants such as crop plants). Examples of plant expression vectors include Becker, D., Kemper, E., Schell, J. and Masterson, R. (1992) "New plant binary vectors with selectable markers located proximal to the left border", Plant Mol . Biol . 20: 1195-1197, Bevan, MW (1984) "Binary Agrobacterium vectors for plant transformation", Nucl . Acid . Res . 12: 8711-8721, including those described above, pLGV23, pGHlac +, pBIN19, pAK2004 and pDH51 (Pouwels et al ., eds. (1985) Cloning Vectors. Elsevier: New York IBSN 0 444 904018).

In another embodiment, a mammalian expression vector is used to express the nucleic acid of the invention in a mammalian cell. Examples of mammalian expression vectors include pCDM8 (see Seed, B. (1987) Nature 329: 840) and pMT2PC (Kaufman et. al . (1987) EMBO J. 6: 187-195). When using mammalian cells, the regulatory function of the expression vector is usually by viral regulatory elements. For example, commonly used promoters are derived from polyoma, adenovirus 2, cytomegalovirus and simian virus 40. Other expression systems suitable for both prokaryotic and eukaryotic cells can be found in Chapters 16 and 17 of Sambrook, J., Fritsh, EF, and Maniatis, T. Molecular Cloning: A Laboratory Manual . 2 nd . ed . Cold Spring Harbor Laboratory , Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989.

In other embodiments, recombinant mammalian expression vectors allow for differential nucleic acid expression in certain cell types (eg, tissue-specific regulatory elements are used to express nucleic acids). Tissue-specific regulatory elements are known in the art. Non-limiting examples of suitable tissue-specific promoters include albumin promoters (liver-specific; Pinkert et. al . (1987) Genes Dev . 1: 268-277), lymphoid-specific promoters (see Calame and Eaton (1988) Adv . Immunol . 43: 235-275), in particular promoters of T cell receptors (Winoto and Baltimore ( 1989) EMBO J. 8: 729-733) and promoters of immunoglobulins (Banerji et. al . (1983) Cell 33: 729-740, see Queen and Baltimore (1983) Cell 33: 741-748), neuron-specific promoters (eg neurofilament promoters; Byrne and Ruddle (1989) PNAS 86: 5473-5477), pancreas-specific promoters (Edlund et al . (1985) Science 230: 912-916) and mammary gland-specific promoters (eg, whey promoter; see US Pat. No. 4,873,316 and European Application No. 264,166). In addition, developmental-regulatory promoters, such as the hox promoter in mice (see Kessel and Gruss (1990) Science 249: 374-379) and the α-fetoprotein promoter (Campes and Tilghman (1989) Genes ) Dev . 3: 537-546).

The present invention further provides a recombinant expression vector containing a DNA molecule of the invention cloned in an expression vector in an antisense direction. That is, the DNA molecule is operably linked to a regulatory sequence to allow expression (by transcription of the DNA molecule) of an RNA molecule that is antisense to SRT mRNA. Regulatory sequences operably linked to the nucleic acid cloned in the antisense direction may be chosen to allow for continuous expression of antisense RNA molecules in various cell types, such as, for example, viral promoters and / or enhancers, or the antisense RNA may be constitutive. Regulatory sequences may also be selected that allow for tissue-specific or cell type specific expression. Antisense expression vectors can be in the form of recombinant plasmids, phagemids or attenuated viruses that produce antisense nucleic acids under the control of highly efficient regulatory regions, the activity of which can be determined by the cell type into which the vector has been introduced. Controlling gene expression using antisense genes is described in Weintraub, H. et. al ., Antisense RNA as a molecular tool for genetic analysis, Reviews -Trends in Genetics , Vol. 1 (1) 1986).

Another aspect of the invention relates to a host cell into which the recombinant expression vector of the invention has been introduced. The terms "host cell" and "recombinant host cell" are used interchangeably herein. Such terms are understood not only to refer to specific subject cells but also to progeny or potential progeny of such cells. In fact, such progeny are not identical to parental cells, although generations may result in mutations or environmental effects over generations, but are still within the scope of the term used herein.

The host cell can be any prokaryotic or eukaryotic cell. For example, the SRT protein can be expressed in bacterial cells such as Corynebacterium glutamicum, insect cells, yeast or mammalian cells (eg, Chinese large mouse ovary cells (CHO) or COS cells). Other suitable host cells are known to those skilled in the art. Corynebacterium glutamicum related microorganisms that can be readily used as host cells for the nucleic acid and protein molecules of the present invention are described in Table 3.

Vector DNA can be introduced into prokaryotic or eukaryotic cells via conventional transformation or transfection techniques. As used herein, the terms “transformation” and “transfection” refer to a heterologous nucleic acid (eg, linear DNA or RNA (eg, a sole genetic construct that is or is not a linearized vector)) or a nucleic acid in the form of a vector (eg, Plasmid, phage, paspid, phagemid, transposon, or other DNA) means a variety of known techniques for introducing into a host cell, simultaneous calcium phosphate or calcium chloride precipitation, DEAE-dextran- trans transfection, lipo Lipofection or electroporation. Suitable methods for transforming or transfecting host cells are described in Sambrook et. al . ( Molecular Cloning : A Laboratory Manual . 2 nd , ed ., Cold Spring Harbor Laboratory , Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989) and other experimental guides.

For stable transfection of mammalian cells, it is known that only a few minor cells insert heterologous DNA into their genome, depending on the expression vector and the transfection technique used. To identify and select these inserts, genes that encode selectable labels (eg, antibiotic resistance) are generally introduced into the host cell along with the gene of interest. Preferred selectable labels include those that confer drug resistance, such as G418, hygromycin and methotrexate. The nucleic acid encoding the selectable label may be introduced into a host cell on the vector encoding the SRT protein, or may be introduced on a separate vector. Cells stably transfected with the introduced nucleic acid can be identified by drug selection (eg, cells incorporating selectable marker genes will survive, but other cells will die).

To prepare homologous recombinant microorganisms, a vector containing at least a portion of the SRT gene that has been modified (eg, disrupted in function) by the deletion, addition or substitution is introduced. Preferably this SRT gene is a Corynebacterium glutamicum SRT gene, but may be a homologue from mammal, yeast or insect origin as well as homologues from related bacteria. In a preferred embodiment, the vector is designed such that by homologous recombination the endogenous SRT gene is functionally disrupted (ie, no longer encodes a functional protein, also referred to as a "knock-out" vector). Alternatively, the vector is designed to encode a functional protein (ie, upstream regulatory regions can be modified so that expression of the endogenous SRT protein can be modified) by homologous recombination where the endogenous SRT gene has been mutated or otherwise modified. In homologous recombination vectors, additional nucleic acids of the SRT gene are located on the 5 'and 3' sides of the modified SRT gene fragment to allow homologous recombination between the foreign SRT gene carried by the vector and the endogenous SRT gene in the microorganism. The added flanking SRT nucleic acid is of sufficient length to successfully homologously recombine with the endogenous gene. Typically, several kilobases of flanking DNA (both 5 'and 3' ends) are included in the vector (eg, Thomas, KR, and Capecchi, MR (1987), which describe homologous recombination vectors). Cell 51: 503). The vector is introduced into the microorganism (e.g., by electroporation), and cells in which the introduced SRT gene and the endogenous SRT gene are homologously recombined are selected by known techniques.

In other embodiments, the recombinant microorganism may be prepared by including a selection system to regulate the expression of the introduced gene. For example, if the SRT gene is located under the control of the lac operon and included on the vector, expression of the SRT gene is possible only in the presence of IPTG. Such control systems are known in the art.

In other embodiments, the endogenous SRT gene in the host cell is disrupted (eg, by homologous recombination or other genetic means known in the art) such that expression of its protein product does not occur. In other embodiments, the SRT gene introduced into an endogenous or host cell is modified by one or more point mutations, deletions or inversions, but still encodes a functional SRT protein. In another embodiment, one or more regulatory regions (eg, promoters, refreshers or inducers) in the microorganism are modified (eg, by deletions, cleavage, inversion or point mutations) to alter the expression of the SRT protein. Those skilled in the art will appreciate that host cells containing one or more of the SRT genes and protein modifications described above can be readily constructed using the methods of the present invention and are included in the present invention.

Host cells of the invention, such as cultured prokaryotic or eukaryotic host cells, produce (ie, express) SRT proteins. Accordingly, the present invention further provides a method for producing SRT protein using the host cell of the present invention. In one embodiment, the method comprises the steps of introducing a host cell of the invention (recombinant expression vector encoding an SRT protein or a gene encoding a wild type or modified SRT protein introduced into the genome) until the SRT protein is produced. Culturing in a suitable medium. In another embodiment, the method further comprises isolating SRT protein from the medium or host cell.

C. Isolated SRT Protein

Another aspect of the invention relates to an isolated SRT protein and a biologically active fragment thereof. An "isolated" or "purified" protein or biologically active fragment thereof is substantially free of cellular material when produced by recombinant DNA technology, or substantially free of chemical precursors or other chemicals when chemically synthesized . The term "substantially free of cellular material" includes the preparation of an SRT protein which has isolated said protein from the cellular components of cells which produce SRT proteins, either naturally or recombinantly. In one embodiment, the term "substantially free of cellular matter" means that the non-SRT protein (also referred to herein as "pollutant protein") content is less than about 30% (dry weight), preferably less than about 20%, More preferably less than about 10%, most preferably about 5%. It is also preferred if the SRT protein or biologically active fragment thereof is produced recombinantly, substantially free of culture medium. In other words, protein preparations with a culture medium content of less than about 20% by volume, more preferably less than about 10% by volume and most preferably less than 5% by volume are preferred. The term "substantially free of chemical precursors or other chemicals" includes preparation of SRT proteins that separate the proteins from chemical precursors or other chemicals involved in the synthesis of SRT proteins. In one embodiment, the term “substantially free of chemical precursors or other chemicals” means that the content of the chemical precursor or non-SRT chemical is less than about 30% (dry weight), preferably less than about 20%, more preferably. Preferably less than about 10%, most preferably about 5%. In a preferred embodiment, the isolated protein or biologically active fragment thereof is free of contaminating protein from the organism from which the SRT protein is derived. Typically, such proteins are produced by recombinant expression of Corynebacterium glutamicum SRT proteins in microorganisms such as, for example, Corynebacterium glutamicum.

Isolated SRT proteins or portions thereof of the present invention may contribute to the resistance or resistance of Corynebacterium glutamicum to one or more chemical or environmental stresses or risks, or may have one or more activities listed in Table 1 have. In a preferred embodiment, the protein or portion thereof is capable of maintaining the ability to mediate the resistance or resistance of Corynebacterium glutamicum to one or more chemical or environmental stresses or risks (e.g., And an amino acid sequence having sufficient homology with an even numbered sequence number in the sequence listing). Some of these proteins are preferably biologically active fragments as described herein. In another preferred embodiment, an SRT protein of the invention has an amino acid sequence described in a sequence having an even sequence number in the sequence listing. In another preferred embodiment, the SRT protein has an amino acid sequence encoded by a nucleotide sequence that hybridizes (eg, hybridizes under stringent conditions) to a nucleotide sequence of the present invention (e.g., sequences having an odd sequence number in the sequence listing). . In another preferred embodiment, the SRT protein has about 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, homology with one of the nucleic acid sequences of the invention or fragments thereof, 58%, 59% or 60% or more, preferably about 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69% or 70% or more, more preferably about 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79% or 80% or more, 81%, 82%, 83%, 84%, 85%, 86%, 87 %, 88%, 89% or 90% or more, 91%, 92%, 93%, 94% or more, more preferably about 95%, 96%, 97%, 98%, 99% or more Has an amino acid sequence encoded by the sequence. Also included in the present invention is a range of identity values that are intermediate to the aforementioned values (eg, 70-90% identity or 80-95% identity). For example, the range of identity values using any of the above mentioned values in combinations of maximum and / or minimum values is encompassed by the present invention. In addition, preferred SRT proteins of the present invention preferably retain one or more SRT activities described herein. For example, preferred SRT proteins of the present invention hybridize to the nucleotide sequences of the present invention, for example, hybridize under stringent conditions and resist or resist Corynebacterium glutamicum to one or more environmental or chemical stresses. Or amino acid sequences encoded by nucleotide sequences having one or more activities listed in Table 1.

In other embodiments, the SRT protein is substantially homologous to an amino acid sequence of the invention (e.g., a sequence with an even sequence number in the sequence listing), and the amino acid sequence is altered due to natural variation or mutation as described above in subsection I. Otherwise retains the functional activity of a protein of one of the amino acid sequences of the invention. Thus, in another embodiment, the SRT protein has about 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59 homology with the entire amino acid sequence of the present invention. % Or 60% or more, preferably about 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69% or 70% or more, more preferably about 71%, 72 %, 73%, 74%, 75%, 76%, 77%, 78%, 79% or 80% or more, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88% At least 89% or 90%, or 91%, 92%, 93%, 94%, more preferably about 95%, 96%, 97%, 98%, 99% or more , One or more of the SRT activities described herein. Also included in the present invention is a range of identity values that are intermediate to the aforementioned values (eg, 70-90% identity or 80-95% identity). For example, the range of identity values using any of the above mentioned values in combinations of maximum and / or minimum values is encompassed by the present invention. In another embodiment, the present invention relates to a full length Corynebacterium glutamicum protein that is substantially homologous to the entire amino acid sequence of the present invention.

Biologically active fragments of SRT proteins include amino acid sequences derived from amino acid sequences of SRT proteins (e.g., sequences with even sequence numbers in the sequence listing) or include amino acid sequences of proteins homologous to SRT proteins and full length SRT proteins. Or it is homologous to SRT protein and contains fewer amino acids than full-length protein that exhibits one or more SRT protein activities. Typically, biologically active fragments (peptides, eg, peptides having amino acid lengths of 5, 10, 15, 20, 30, 35, 36, 37, 38, 39, 40, 50, 100 or more) are SRT Domains or motifs having one or more activities of the protein. In addition, fragments having different biologically active activities due to deletion of other regions of the protein can be produced by recombinant techniques and can measure one or more of the activities described herein. Preferably, the biologically active fragment of the SRT protein comprises one or more selected domains / motifs or fragments thereof that are biologically active.

SRT proteins are preferably produced by recombinant DNA technology. For example, the nucleic acid molecule encoding the protein is cloned into an expression vector (as described above), the expression vector is introduced into a host cell (as described above), and the SRT protein is introduced into the host cell. Expressed. The SRT protein can then be isolated from the cells through appropriate purification steps using standard protein purification techniques. Apart from recombinant expression, SRT proteins, polypeptides or peptides can be chemically synthesized using standard peptide synthesis techniques. In addition, native SRT proteins can be isolated from cells (eg, endothelial cells) using, for example, anti-SRT antibodies that can be produced by standard methods using the SRT proteins or fragments of the invention.

The present invention also provides an SRT chimeric or fusion protein. As used herein, SRT “chimeric protein” or “fusion protein” includes SRT polypeptides operably linked to non-SRT polypeptides. "SRT polypeptide" refers to a polypeptide having an amino acid sequence corresponding to an SRT, and a "non-SRT polypeptide" refers to a protein that is substantially homologous to an SRT protein, such as a SRT protein and that is derived from the same or different organisms. A polypeptide having an amino acid sequence corresponding to a protein. In fusion proteins, the term “operably linked” means that the SRT polypeptide and the non-SRT polypeptide are fused to each other in frame. The non-SRT polypeptide may be fused to the N-terminus or C-terminus of the SRT polypeptide. For example, in one embodiment, the fusion protein is a GST-SRT fusion protein in which the SRT sequence is fused to the C-terminus of the GST sequence. Such fusion proteins can facilitate the purification of recombinant SRT proteins. In another embodiment, the fusion protein is an SRT protein containing a heterologous signal sequence at its N-terminus. In certain host cells (eg, mammalian host cells), expression and / or secretion of SRT proteins can be increased by using heterologous signal sequences.

Preferably, the SRT chimeric or fusion proteins of the invention are produced by standard recombinant DNA techniques. For example, DNA fragments encoding separate polypeptide sequences may be used according to conventional techniques, for example using blunt-ended or crossed ends for ligation, processing restriction enzymes to make appropriate ends, and If complementary ends are filled and alkaline phosphatase is treated to avoid undesirable linkage and ligated together in the same frame by ligation with enzymes. In other embodiments, the fusion gene can be synthesized by conventional techniques, including automated DNA synthesizers. Alternatively, amplification primers can be used to perform PCR amplification of gene fragments, which cause complementary overhangs between two consecutive gene fragments, which are subsequently annealed and reamplified to produce chimeric gene sequences. (See, eg, Current Protocols in Molecular Biology, eds. Ausubel et . al . John Wiley & Sons: 1992). In addition, many expression vectors that encode fusion residues (eg, GST polypeptides) are commercially available. SRT encoding nucleic acids can be cloned into such expression vectors such that the fusion residues can be linked in the same frame to the SRT protein.

Homologs of SRT proteins can be produced by mutations, for example fractional point mutations or cleavage of the SRT protein. As used herein, “homolog” refers to a variant form of SRT protein that acts as an agonist or antagonist to the activity of the SRT protein. The agonist of the SRT protein may have substantially the same or similar biological activity as the SRT protein. Antagonists of SRT proteins may inhibit one or more activities of native SRT proteins by competitively binding to, for example, downstream or upstream members of the SRT system comprising the SRT protein. Thus, the Corynebacterium glutamicum SRT protein and its homologues can increase the resistance or resistance of Corynebacterium glutamicum to one or more chemical or environmental stresses.

In other embodiments, homologues of the SRT protein can be identified by screening combinatorial libraries of mutations (eg, cleavage mutations) of the SRT protein for SRT protein agonist or antagonist activity. In one embodiment, a library of mixed SRT variants may, for example, enzymatically ligate a synthesized oligonucleotide mixture into a gene sequence such that a degenerate set of potential SRT sequences can be expressed as individual polypeptides or alternatively therein. It can be made expressible as a large fusion protein (eg, phage display) containing a set of sequences. There are a variety of methods available for producing libraries of potential SRT homologues from degenerate oligonucleotide sequences. Chemical synthesis of degenerate gene sequences can be performed in an automated DNA synthesizer, and then the synthetic genes can be ligated into appropriate expression vectors. Gene degeneracy sets can be used to store all sequences encoding a desired set of potential SRT sequences in one mixture. Methods for synthesizing degenerate oligonucleotides are known in the art (eg, Narang, SA (1983) Tetrahedron 39: 3, Itakura et. al . (1984) Annu. Rev. Biochem. 53: 323, Itakura et. al . (1983) Science 198: 1056, Ike et al . (1983) Nucleic Acid Res. 11: 477].

In addition, fragment libraries of SRT protein coding genes can be used to generate a mixed population of SRT fragments for screening and subsequent selection of homologs of SRT proteins. In one embodiment, the nuclease is treated to a double-stranded PCR fragment of the SRT coding sequence, the double-stranded DNA is denatured, and the sense / antisense from the other notch product under conditions in which nicking occurs about once per molecule. Coding by degenerating the DNA to form double stranded DNA, which may include pairs, treating S nuclease to remove the single stranded portion from the reformed double strand, and ligating the resulting fragment library into the expression vector. Libraries of sequence fragments can be prepared. By this method, expression libraries encoding N-terminal, C-terminal and internal fragments of SRT proteins of various sizes can be derived.

Several techniques are known in the art for screening for gene products in combinatorial libraries made by point mutations or cleavage, and for screening for gene products having properties selected from cDNA libraries. Such techniques can be used to quickly screen gene libraries generated by combinatorial mutations of SRT homologues. Among the most widely used techniques, techniques available for high throughput assays for screening large gene libraries typically include cloning the gene library into replicable expression vectors, transforming the appropriate cells with the generated vector library, and Detection of activity includes expressing a combination gene under conditions that facilitate the isolation of the vector encoding the gene product to be detected. Recursive ensemble mutagenesis (REM), a new technique that increases the frequency of functional mutations in a library, can be used in combination with screening assays to identify SRT homologues (Arkin and Yourvan (1992). ) PNAS 89: 7811-7815, Delgrave et. al . (1993) Protein Engineering 6 (3): 327-331).

In other embodiments, cell based assays can be used to analyze various SRT libraries using methods well known in the art.

D. Uses and Invention of the Invention

Nucleic acid molecules, proteins, protein homologues, fusion proteins, primers, vectors and host cells described herein may be used to identify corynebacterium glutamicum and related organisms, genomic mapping of organisms associated with corynebacterium glutamicum, Identification and location of target sequences of Corynebacterium glutamicum, evolutionary studies, determination of SRT protein sites required for function, regulation of SRT protein activity, regulation of SRT protein pathway activity, and the preparation of target compounds such as fine chemicals. It can be used for one or more of methods such as cell production regulation.

SRT nucleic acid molecules of the present invention have a variety of uses. First, it can be used to identify the presence of Corynebacterium glutamicum or its close homologues. It can also be used to confirm the presence of Corynebacterium glutamicum or its close homologues in a mixed microbial population. The present invention relates to a large number of cory that can be identified by presence of genomic DNA extracted from single or mixed microbial cultures using probes spanning gene regions unique to Corynebacterium glutamicum under stringent conditions. Nucleic acid sequences of the Nebacterium glutamicum gene are provided.

Corynebacterium glutamicum itself is not pathogenic, but is associated with Corynebacterium diphtheriae. Corynebacterium diphtheria is the causative agent of diphtheria, a rapidly developing acute fever infection with local and systemic lesions. In these diseases, local lesions develop on the upper respiratory tract, accompanied by necrotic damage to epithelial cells, and Bacillus secretes toxins, which spread through the lesion to tissues susceptible to infection. Regressive planetary changes caused by inhibition of protein synthesis in such tissues, including the heart, muscle peripheral nerves, adrenal glands, kidneys, liver and spleen, cause systemic pathology of the disease. Diphtheria has a high incidence in many parts of the world, including Africa, Asia, Eastern Europe and the independent states of the former Soviet Union. The epidemic diphtheria, which continues in eastern Europe and the Soviet Union's independent states, has caused more than 5,000 deaths since 1990.

In one embodiment, the present invention provides a method for identifying the presence or activity of Corynebacterium diphtheria in a subject. The present invention includes detecting the presence or activity of Corynebacterium diphtheria in a subject by detecting one or more nucleic acid or amino acid sequences of the invention (represented by odd or even sequence numbers in the sequence listing, respectively) in the subject. Corynebacterium glutamicum and Corynebacterium diphtheria are related bacteria, and many of the nucleic acid and protein molecules of Corynebacterium glutamicum have homology with the nucleic acid and protein molecules of Corynebacterium diphtheriere. It can be used to detect Corynebacterium diphtherinee in a subject.

In addition, nucleic acid and protein molecules of the invention function as markers of specific regions of the genome. This function is useful not only for genome mapping but also for the study of the function of Corynebacterium glutamicum protein. For example, the Corynebacterium glutamicum genome can be cut to incubate fragments with DNA binding proteins to identify genomic regions to which specific DNA binding proteins of Corynebacterium glutamicum bind. The region that binds to the protein can also be probed with a nucleic acid molecule of the invention, preferably a label that can be easily detected. The binding of nucleic acid molecules to such genomic fragments allows the fragments to be located on the genomic map of Corynebacterium glutamicum and, if performed multiple times with different enzymes, quickly and easily determines the nucleic acid sequences to which the proteins bind. do. In addition, the nucleic acid molecules of the present invention may have sufficient homology with the sequences of the associated species and can be used as markers for constructing genomic maps of associated bacteria, such as Brevibacterium lactofermentum.

In addition, the SRT nucleic acid molecules of the present invention are useful for evolution and protein structure studies. The resistance process involving the molecules of the present invention is used by a wide variety of cells and the evolutionary association of an organism can be assessed by comparing the sequence of nucleic acid molecules of the present invention with nucleic acid molecule sequences encoding enzymes similar to other organisms. Similarly, such comparisons can assess whether a sequence region is conserved, which can help determine protein regions essential for enzyme function. This method of determination is useful for protein engineering and can indicate whether it can tolerate induced mutations without loss of protein function.

A gene of the invention, eg, a gene encoding LMRB (SEQ ID NO: 1), or another gene of the invention encoding a chemical or environmental resistant or resistant protein (eg, resistance to one or more antibiotics) is coryne It can be used as a genetic marker for genetic transformation of an organism such as bacterium glutamicum or other bacterial species (eg, disruption of an existing gene or transfer of another gene into an existing gene). The use of these nucleic acid molecules on the basis of the properties that allow the organism to survive in an inadequate or toxic environment (eg, in the presence of an antimicrobial compound) allows the use of the corresponding transgene cassette (e.g., Phagemids, transposons or other nucleic acid elements) can be efficiently selected. The use of one or more genes of the invention as genetic markers improves the speed and ease with which genetically engineered and isolated organisms with the desired transformation properties (eg, production of controlled fine chemicals) are genetically engineered. It is also beneficial to use the genes of the invention for the selection of transformed Corynebacterium glutamicum and related bacteria, but as described herein, homologues (eg, homologues from other organisms), alleles It is also possible to use genetic variants or fragments of genes possessing the desired activity. In addition, the 5 'and 3' regulatory elements of the genes of the invention can be modified as described herein (eg, nucleotide substitutions, insertions, deletions, or replacements with more preferred genetic elements) to regulate transcription of the gene. . For example, one can use LMRB variants in which the nucleotide sequence in the region ranging from -1 to -200 5 'to the start codon is mutated to (preferably increase) transcription and / or translation of the LMRB. This variant is functionally coupled to one or more regulatory signals (eg, inducer or repressor binding sequence) in which the gene of the present invention (eg, LMRB gene (SEQ ID NO: 1)) can be used to regulate gene expression. It may be a ring structure. Similarly, one or more copies of the genes of the invention can be used.

Other uses of the genes of the invention (e.g., genes encoding LMRB (SEQ ID NO: 1) or other drug- or antibiotic-resistant genes) can be used for new antibiotics having activity against Corynebacterium and / or other bacteria. Is in discovery. For example, the genes of the present invention may be expressed (or overexpressed) in a host suitable for generating organisms with increased resistance to one or more drugs or antibiotics (specifically for LMRB, lycosamide, especially rincomycin). You can. This type of resistant host can then be used to screen for chemicals with bacteriostatic and / or bactericidal activity, such as novel antibiotic compounds. In particular, it is possible to identify novel antibiotics having activity against microorganisms already resistant to standard antibiotic compounds using the genes of the invention (eg LMRB genes).

The present invention provides a method for screening molecules that modulate the activity of the SRT protein by interacting the SRT protein itself with its substrate or binding partner, or by regulating the transcription and translation of the SRT nucleic acid molecule of the invention. In this method, microorganisms expressing at least one SRT protein of the invention are contacted with at least one test compound and the effect of each test compound on the activity or expression of the SRT protein is evaluated.

The manipulation of the SRT nucleic acid molecules of the present invention can produce SRT proteins with functions different from wild-type SRT proteins. Such proteins may be improved in efficiency or activity, may be present in excess than normal cells, or may be reduced in efficiency or activity. The purpose of this manipulation is to increase the viability and activity of cells when they are exposed to environmental and chemical stresses and risks often encountered in large-scale fermentation cultures. Thus, by increasing the activity or copy number of a heat-shock-regulating protease, it interferes with normal cellular functioning (eg, by continuing to bind to a substrate or cofactor, even if the protein does not have the activity to properly act on these molecules). It can increase the cell's ability to destroy incorrectly folded proteins. This can increase the cell's ability as described above directly even when optimal for overexpression or activity of one or more chaperon molecules induced by heat or cold shock. These proteins assist in the correct folding of the neonatal polypeptide chains, such that increased activity or presence of these proteins increases the proportion of correctly folded proteins in the cells, thereby increasing the overall metabolic efficiency and viability of the cells in culture. will be. Overexpression or optimization of transporter molecules activated by osmotic shock increases the ability of some cells to maintain intracellular acidity, thereby increasing the viability of these cells in culture. Similarly, overproduction or increased activity by mutagenesis of proteins involved in the development of cellular resistance to various types of chemical stress (by transferring harmful chemicals outside the cell, or by transforming chemicals into less harmful substances). Can increase the adaptability of organisms in environments containing harmful substances (eg, large scale fermentation cultures), thereby relatively increasing the number of cells that survive in such cultures. The overall effect of all these mutagenesis methods is to increase the amount of fine chemical producing compound in the culture, thereby increasing the yield, productivity and / or production efficiency of one or more of the desired fine chemicals from the culture.

Such mutagenesis methods of SRT proteins for increasing the yield of the desired compound are not limited, and variations of these mutagenesis methods will be apparent to those skilled in the art. By these mechanisms, the nucleic acid and protein molecules of the invention are used to prepare Corynebacterium glutamicum or related strains expressing mutated SRT nucleic acid and protein molecules, yielding, producing and / or producing the desired compounds. Improve the efficiency. The compound of interest is a coryne including a molecule produced by the Corynebacterium glutamicum strain, which does not inherently occur in Corynebacterium glutamicum metabolism, as well as intermediates of the biosynthetic pathway and intermediates of natural metabolic pathways. It can be any product produced by bacterium glutamicum.

In addition, the present invention is illustrated by the following examples, but should not be construed as being limited thereto. All references, patent applications, patents, all contents of published patent applications, tables and sequences listed herein are incorporated by reference.

TABLE 1

Genes Included in this Application

TABLE 2

Gene excluded from the present invention

TABLE 3

TABLE 4

EXAMPLE

Example  One: Corynebacterium Glutamicum ATCC  13032 whole genome DNA of Prep

Corynebacterium glutamicum (ATCC 13032) cultures in BHI medium (Difco) were grown overnight with vigorous shaking at 30 ° C. Cells were harvested by centrifugation to discard the supernatant and cells were resuspended in 5 ml Buffer-I (corresponding to 5% of the initial volume of culture—all volumes indicated for 100 ml of culture volume). The composition of Buffer-I was as follows: 140.34 g / L sucrose, 2.46 g / L MgS0 ₄ × 7 H ₂ O, 10 ml / L KH ₂ PO ₄ solution (100 g / L, pH 6.7 using KOH) ), 50 ml / L M12 concentrate (10 g / L (NH ₄ ) ₂ SO ₄ , 1 g / L NaCl, 2 g / L MgSO ₄ × 7 H ₂ O, 0.2 g / L CaCl ₂ , 0.5 g / L yeast extract (from Difco), 10 ml / L trace element mixture (200 mg / L FeSO ₄ × H ₂ O, 10 mg / L ZnSO ₄ × 7 H ₂ O, 3 mg / L MnCl ₂ × 4 H ₂ O, 30 mg / LH ₃ BO ₃ , 20 mg / L CoCl ₂ × 6 H ₂ O, 1 mg / L NiCl ₂ × 6 H ₂ O, 3 mg / L Na ₂ MoO ₄ × 2 H ₂ O, 500 mg / L complexing agent (EDTA or citric acid), 100 ml / L vitamin mixture (0.2 mg / L biotin, 0.2 mg / L folic acid, 20 mg / L p-amino benzoic acid, 20 mg / L riboflavin, 40 mg / L ca-pantothenate, 140 mg / L nicotinic acid, 40 mg / L pyridoxol hydrochloride, 200 mg / L myo-inositol). Lysozyme was added to the suspension at a final concentration of 2.5 mg / ml. Protoplasts were harvested by incubating at 37 ° C. for approximately 4 hours, then breaking the cell walls and centrifuging. The pellet was washed once with 5 ml Buffer-I and once with 5 ml TE-buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8). The pellet was resuspended in 4 ml TE-buffer and 0.5 ml SDS solution (10%) and 0.5 ml NaCl solution (5 M) were added. Proteinase K was added at a final concentration of 200 μg / ml, and then the suspension was incubated at 37 ° C. for about 18 hours. DNA was purified by extraction with phenol, phenol-chloroform-isoamyl alcohol and chloroform-isoamyl alcohol using standard methods. The DNA was then precipitated by addition of 1/50 volume of 3 M sodium acetate and 2 volumes of ethanol, followed by incubation at −20 ° C. for 30 minutes, followed by a high speed centrifuge using an SS34 rotor (Sorvall). Centrifugation for 30 minutes at 12,000 rpm. The DNA was dissolved in 1 ml TE-buffer containing 20 μg / ml RNaseA and dialyzed in 1000 ml TE-buffer at 4 ° C. for at least 3 hours. During dialysis, the buffer was changed three times. 0.4 ml aliquots of the dialyzed DNA solution were added, and 0.4 ml 2 M LiCl and 0.8 ml ethanol were added. DNA was collected by incubation at −20 ° C. for 30 minutes and then centrifuged (13,000 rpm, Biofuge Fresco, Hanau Heraeus, Germany). DNA pellets were dissolved in TE-buffer. DNA prepared in this manner was used for all purposes, such as Southern blotting or genome library construction.

Example 2: In Escherichia Coli Corynebacterium Glutamicum Genome Library Construction of ATCC13032

Using prepared DNA as described in Example 1, known and established methods (eg, Sambrook, J. et al. (1989) "Molecular Cloning: A Laboratory Manual", Cold Spring Harbor Laboratory) Presses or see Ausubel, FM et al. (1994) "Current Protocols in Molecular Biology", John Wiley & Sons) to produce cosmid and plasmid libraries.

Any plasmid or cosmid could be used. In particular, plasmid pBR322 (Sutcliffe, J.G. (1979) Proc. Natl. Acad. Sci. USA, 75: 3737-3741); pACYC177 (Change & Cohen (1978) J. Bacteriol 134: 1141-1156), plasmids of the pBS family (pBSSK +, pBSSK-, etc .; Stratagene, LaJolla, USA), or SuperCos I (Stratagene, LaJolla, USA) or Lorist 6 (Gibson, TJ, Rosenthal A. and Waterson, RH (1987) Gene 53: 283-286). Specifically, the gene library for use in Corynebacterium glutamicum is plasmid pSL 109 (Lee, H.-S. and AJ Sinskey (1994) J. Microbiol. Biotechnol. 4: 256-263). Can be produced using.

Example  3: DNA  Sequencing and Functional Analysis Using Computers

Using a genomic library as described in Example 2, DNA sequencing according to standard methods, in particular chain termination methods using ABI377 sequencers (see, eg, Fleischman, RD et al. (1995) "Whole- DNA sequencing was performed by genome Random Sequencing and Assembly of Haemophilus Influenzae Rd., Science, 269: 496-512.Sequencing primers with the following nucleotide sequences were used: 5'-GGAAACAGTATGACCATG-3 ' 305) or 5'-GTAAAACGACGGCCAGT-3 '(SEQ ID NO: 306).

Example  4: In vivo  Mutagenesis

Corynebacterium glutamicum vivo mutagenesis is a complete genetic information, ability to maintain a corrupt E. coli or other microorganisms (eg Bacillus species (Bacillus spp.), Or Saccharomyces access to my Celebi jiae (Saccharomyces yeast) such as cerevisiae ) may be performed by passage of plasmid (or other vector) DNA. Typical mutagenesis strains include genes related to DNA repair systems (eg mutHLS, mutD, mutT, etc .; for reference, see Rupp, WD (1996) DNA repair mechanisms, in: Escherichia coli and Salmonella , p. 2277- 2294, ASM: Washington). Such strains are known to those skilled in the art. The use of such strains is described, for example, in Greener, A. and Callahan, M. (1994) Strategies 7: 32-34.

Example  5: coliform Corynebacterium Glutamicum  Between DNA  relay

Several Corynebacterium and Brevibacterium species contain endogenous plasmids that autonomously replicate (eg, pHM1519 or pBL1, etc.) (see, for example, Martin, JF et al. (1987) Biotechnology, 5: 137-146). Shuttle vectors for use with E. coli and Corynebacterium glutamicum are standard vectors used for E. coli, with the origin of replication for Corynebacterium glutamicum and the appropriate markers from Corynebacterium glutamicum Sambrook, J. et al. (1989), "Molecular Cloning: A Laboratory Manual", Cold Spring Harbor Laboratory Press, or Ausubel, FM et al. (1994) "Current Protocols in Molecular Biology", John Wiley & Sons]). The origin of replication is preferably obtained from endogenous plasmids isolated from Corynebacterium and Brevibacterium species. In particular, the gene used as a transformation marker for the species may be a kanamycin resistance gene (eg, derived from Tn5 or Tn903 transposon, etc.) or chloramphenicol resistance gene (Winnacker, EL (1987) "From Genes to Clones-Introduction". to Gene Technology, VCH, Weinheim] .There are numerous documents on the construction of a wide variety of shuttle vectors that can be cloned in both E. coli and Corynebacterium glutamicum and used for a variety of purposes, including overexpression of genes. For reference, see, eg, Yoshihama, M. et al. (1985) J. Bacteriol. 162: 591-597, Martin JF et al. (1987) Biotechnology, 5: 137-146, and literature. (Eikmanns, BJ et al. (1991) Gene, 102: 93-98).

Using standard methods, the gene of interest can be cloned into one of the shuttle vectors described above and this hybrid vector can be introduced into the Corynebacterium glutamicum strain. Transformation of Corynebacterium glutamicum is known as protoplast transformation (Kastsumata, R. et al. (1984) J. Bacteriol. 159: 306-311), electroporation (Liebl, E. et al. (1989) FEMS Microbiol.Letters, 53: 399-303) and conjugation methods when certain vectors are used (eg, Schaefer, A et al. (1990) J. Bacteriol. 172: 1663). -1666). In addition, prep the plasmid DNA from Corynebacterium glutamicum (using standard methods known in the art) and transform it into Escherichia coli to transfer the shuttle vector to E. coli for use in Corynebacterium glutamicum. It may be. This transformation step can be performed using standard methods, but using a Mcr-deficient Escherichia coli strain such as NM522 (Gough & Murray (1983) J. Mol. Biol. 166: 1-19) and the like. It is advantageous.

pCGl (US Pat. No. 4,617,267) or fragments thereof, and optionally kanamycin resistance genes from TN903 (Grindley, ND and Joyce, CM (1980) Proc. Natl. Acad. Sci. USA 77 (12 ): 7176-7180]) can be used to overexpress genes in Corynebacterium glutamicum strains. In addition, plasmid pSL 109 (Lee, H.-S. and AJSinskey (1994) J. Microbiol. Biotechnol. 4: 256-263) was used to overexpress genes in Corynebacterium glutamicum strains. Can be.

In addition to using a replicative plasmid, gene overexpression may also be achieved by integration into the genome. Genomic integration in Corynebacterium glutamicum or other Corynebacterium or Brevibacterium species is characterized by homologous recombination with genomic region (s), restriction endonuclease mediated integration (REMI) (eg, literature (See German Patent No. 19823834), or by known methods such as using transposon. In addition, regulatory regions (e.g., by deletion, insertion, or deletion of sequences using positioning methods (e.g., homologous recombination) or random event based methods (e.g., transposon mutagenesis or REMI) For example, promoters, repressors, and / or enhancers may be modified to modulate the activity of the gene of interest. In addition, a nucleic acid sequence that functions as a transcription terminator may be inserted into one or more coding regions 3 'of a gene of the present invention; Such terminators are known in the art and are described, for example, in Winnacker, E.L. (1987) From Genes to Clones-Introduction to Gene Technology. VCH: Weinheim.

Example  6: expression evaluation of mutant proteins

Observation of the activity of the mutated protein in the transformed host cell revealed that the mutant protein was expressed in a similar manner and in a similar amount to the wild type protein. Useful methods for determining the level of transcription of a mutant gene (an indicator of the amount of mRNA available for translation into a gene product) include Northern blotting (see, for example, Ausubel et al. (1988) Current Protocols in Molecular). Biology, Wiley: New York), which extracts the whole RNA of a biological culture, develops it on a gel, transfers it to a stable matrix, and then binds to the gene of interest to detect a tag (usually Incubating with a primer labeled with a radioactive or chemiluminescent tag), the binding of the probe and its binding amount also indicate the presence and amount of mRNA of the gene of interest. This information is evidence of the degree of transcription of the mutant gene. Intracellular total RNA of Corynebacterium glutamicum is described, for example, in Bormann, E.R. et al. (1992) Mol. Microbiol. 6: 317-326 can be prepared using a variety of methods known in the art.

To assess the presence or relative amount of a protein translated from this mRNA, standard techniques such as western blotting can be used (see, for example, Ausubel et al. (1988) Current Protocols in Molecular Biology, Wiley). New York). In this method, the whole protein in the cell is extracted, separated by gel electrophoresis, and then transferred to a matrix such as nitrocellulose and incubated with a probe such as an antibody that specifically binds to a desired protein. Typically, these probes are tagged with chemiluminescent or colorimetric labels that can be easily detected. The presence and amount of the label observed indicates the presence and amount of the desired mutant protein in the cell.

Example 7: Genetically Modified Corynebacterium Growth of Glutamicum -Medium and Culture Conditions

Genetically modified corynebacteria are cultured in synthetic or natural growth media. Numerous different growth media of Corynebacteria are known in the art and are also readily available (Lieb et al. (1989) Appl. Microbiol. Biotechnol., 32: 205-210, von der Osten (1998) Biotechnology Letters, 11: 11-16, German Patent No. 4,120,867, Liebl (1992) "The Genus Corynebacterium", in: The Procaryotes, Volume II, Balows, A. et. al., eds. Springer-Verlag]. The medium consists of one or more carbon sources, nitrogen sources, inorganic salts, vitamins and trace elements. Preferred carbon sources are sugars such as monosaccharides, disaccharides, or polysaccharides. For example, glucose, fructose, mannose, galactose, ribose, sorbose, ribulose, lactose, maltose, sucrose, raffinose, starch or cellulose serve as very good carbon sources. In addition, sugar may be supplied to the medium through complex compounds such as molasses or other by-products generated during sugar purification. It may also be advantageous to feed a mixture of different carbon sources. Other possible carbon sources are alcohols and organic acids such as methanol, ethanol, acetic acid or lactic acid. Nitrogen sources are typically organic or inorganic nitrogen compounds, or materials containing such compounds. Examples of nitrogen sources include ammonia gas or ammonia salts such as NH ₄ Cl or (NH ₄ ) ₂ SO ₄ , NH ₄ 0H, nitrates, ureas, amino acids or corn liquor, soy flour, soy protein, yeast extract, Complex nitrogen sources such as meat extracts.

Inorganic salt compounds that may be included in the medium include calcium, magnesium, sodium, cobalt, molybdenum, potassium, manganese, zinc, copper and iron hydrochlorides, phosphates, or sulfates. Chelating compounds can be added to the medium to keep metal ions in solution. Particularly useful chelating compounds include dihydroxyphenols such as catechol or protocatechuate, organic acids such as citric acid, and the like. Also, the medium typically contains other growth factors such as vitamins or growth promoters, examples of which include biotin, riboflavin, thiamine, folic acid, nicotinic acid, pantophenate and pyridoxine. Growth factors and salts often occur in complex media components such as yeast extract, molasses, corn soak and the like. The exact composition of the media compound will vary greatly with subsequent experiments and will be determined separately for each particular case. Information on media optimization can be found in Applied Microbiol. Physiology, A Practical Approach (eds. PM Rhodes, PF Stanbury, IRL Press (1997) pp.53-73, ISBN 0 19 963577 3). It is also possible to select from growth media from the manufacturer, such as Standard 1 (Merck) or BHI (Leads in Grain, DIFCO).

All media components are sterilized by heat treatment (20 minutes at 1.5 bar and 121 ° C.) or sterile filtration. These components can be sterilized all at once or they can be sterilized separately as needed. All media components may be present from the beginning of the culture, or optionally added continuously or batchwise.

Culture conditions are defined separately for each experiment. The temperature should be in the range between 15 ° C and 45 ° C. The temperature can be kept constant or changed during the experiment. The pH of the medium should be in the range of 5 to 8.5, preferably about 7.0, and can be maintained by adding buffer to the medium. Examples of buffers that can be used for this purpose include potassium phosphate buffers and the like. Synthetic buffers such as MOPS, HEPES, ACES, etc. may alternatively or simultaneously be used. It is also possible to keep the pH of the culture constant by adding NaOH or NH ₄ OH during the culture. When complex media components such as yeast extracts are used, many complex compounds have high buffering performance, which may reduce the need for additional buffers. When the fermentor is used for microbial culture, the pH may be controlled using ammonia gas.

Incubation time ranges are typically several hours to several days. This time is chosen so that the amount of product that accumulates in the broth is maximum. The disclosed culture experiments can be performed in a variety of vessels, such as microtiter plates, glass tubes, glass flasks or glass or metal fermenters of different sizes. To screen numerous clones, microorganisms must be cultured in microtiter plates, glass tubes or shake flasks with or without baffles. It is preferable to use the required growth medium by filling 10% (volume ratio) in a 100 ml shake flask. These flasks should be shaken (25 mm amplitude) on a rotary shaker in the speed range 100-300 rpm. The amount of loss due to evaporation can be reduced by keeping the atmosphere wet; Alternatively, the amount of loss due to evaporation must be corrected correctly.

When testing genetically modified clones, unmodified control clones or control clones containing base plasmids without any inserts should also be tested. CM plates incubated at 30 ° C. (10 g / L glucose, 2.5 g / L NaCl, 2 g / L urea, 10 g / L polypeptone, 5 g / L yeast extract, 5 g / L meat extract, 22 g / Cells on agar plates, such as L agar, adjusted to pH 6.8 with 2 M NaOH), and the like are seeded in the medium to an OD ₆₀₀ 0.5-1.5. Medium inoculation is performed by introducing saline suspensions of Corynebacterium glutamicum cells from CM plates or by adding precultures of these bacteria.

Example  8: Regarding the Function of Mutant Proteins In vitro  analysis

Methods of measuring enzyme activity and reaction rate parameters are well established in the art. Experiments for measuring the activity of any given modified enzyme should be tailored to the inactivation of the wild type enzyme and one skilled in the art can do this well. Specific details regarding the structure, reaction rates, principles, methods, and applications of enzymes, as well as a general overview of enzymes and examples of methods for measuring activity of many enzymes, can be found in the following references: Dixon, M. and Webb, EC, (1979) Enzymes. Longmans: London; Fersht, (1985) Enzyme Structure and Mechanism. Freeman: New York, Walsh, (1979) Enzymatic Reaction Mechanisms. Freeman: San Francisco, Prix, NC, Stevens, L. (1982) Fundamentals of Enzymology. Oxford Univ. Press: Oxford, Boyer, PD, ed. (1983) The Enzymes, 3 ^rd ed. Academic Press: New York, Bisswanger, H., (1994) Enzymkinetik, 2 ^nd ed. VCH: Weinheim (ISBN 3527300325), Bergmeyer, HU, Bergmeyer, J., Grassl, M., eds. (1983-1986) Methods of Enzymatic Analysis, 3 rd ed, vol.I-XII, Verlag Chemie:. Weinheim], and the literature [Ullmann's Encyclopedia of Industrial Chemistry ( 1987) vol.A9, "Enzymes". VCH: Weinheim, p.352-363].

Protein activity that binds to DNA can be measured using a variety of well-established methods, such as DNA band-displacement assays (also called gel retardation assays). The effect of these proteins on the expression of other molecules is described in reporter gene analysis (see, eg, Kolmar, H. et al. (1995) EMBO J. 14: 3895-3904) and references cited therein. As shown in FIG. 2). Reporter gene test systems using enzymes such as β-galactosidase, green fluorescent proteins and many others are known and well established for application to both prokaryotic and eukaryotic cells.

Measurement of the activity of membrane transport proteins is described in Gennis, R.B. (1989) "Pores, Channels and Transporters", in Biomembranes, Molecular Structure and Function, Springer: Heidelberg, p. 85-137, 199-234, and 270-322, and the like.

Example  9: Analysis of the Effect of Mutant Proteins on Desired Product Production

The effect of genetic modification on Corynebacterium glutamicum on the production of the desired compound (e.g., amino acid) may be achieved by culturing the modified microorganism under appropriate conditions (e.g., as described above), Media components and / or intracellular components involved in increased production of product (ie, amino acids) can be assessed by analysis. Such analytical techniques are known to those skilled in the art and include analytical chromatography methods such as spectroscopy, thin layer chromatography, various staining, enzymatic and microbiological methods, and high performance liquid chromatography methods (e.g., literature Ullman, Encyclopedia of Industrial Chemistry, vol. A2, p. 89-90 and p. 443-613, VCH: Weinheim (1985), Falllon, A. et al., (1987) "Applications of HPLC in Biochemistry "in: Laboratory Techniques in Biochemistry and Molecular Biology, vol. 17, Rehm et al. (1993) Biotechnology, vol. 3, Chapter III:" Product recovery and purification ", page 469-714, VCH: Weinheim Belter, PA et al. (1988) Bioseparations: downstream processing for biotechnology, John Wiley and Sons, Kennedy, JF and Cabral, JMS (1992) Recovery processes for biological materials, John Wiley and Sons, Shaeiwitz, JA and Henry, JD (1988) Biochemical separations, in: Ulmann's Ency See clopedia of Industrial Chemistry, vol. B3, Chapter 11, page 1-27, VCH: Weinheim, and Dechow, F.J. (1989) Separation and purification techniques in biotechnology, Noyes Publications).

In addition to the determination of the final fermentation product, analysis of the other components of the metabolic pathways used to produce the desired compound, such as intermediates and by-products, as well as the measurement of the overall yield, productivity and / or production efficiency of the compound is possible. Analytical methods include measuring the content of nutrients (eg, sugars, hydrocarbons, nitrogen sources, phosphates and other ions) in the medium, determining the composition and growth of biomass, analyzing the production of common metabolites in biosynthetic pathways, and Gas measurements generated during fermentation. Standard methods for such measurements are described in Applied Microbial Physiology, A Practical Approach, PMRhodes and PFStanbury, eds., IRL Press, p. 103-129, 131-163, and 165-192 (ISBN: 0199635773) and References cited in the literature.

Example  10: Corynebacterium Glutamicum  Purification of desired product from culture

Various methods known in the art can be used to recover the desired product from Corynebacterium glutamicum cells or supernatants of the cultures described above. If the desired product is not secreted from the cells, the cultures can be harvested by low speed centrifugation and the cells can be lysed by standard techniques such as mechanical force or ultrasound. Cell debris is removed by centrifugation and the supernatant portion containing the soluble protein is left for further purification of the desired compound. If the product is secreted from Corynebacterium glutamicum cells, the cultures are centrifuged at low speed to remove the cells, leaving the supernatant portion for further purification.

The supernatant portion by any of the two purification methods is subjected to chromatography using a suitable resin, so that the desired molecule remains on the chromatography resin but no impurities remain in the sample, or the impurities remain in the resin but the desired molecule remains. Does not remain. If desired, these chromatography steps can be repeated using the same or different chromatography resins. Those skilled in the art will be skilled in selecting suitable chromatography resins and applying them most effectively for the purification of particular molecules. The purified product can be concentrated by filtration or ultrafiltration and stored at a temperature where the stability of the product is maximum.

Purification methods known in the art are various and are not limited to the purification methods described above. Such purification techniques are described, for example, in Bailey, J.E. & Ollis, D.F. Biochemical Engineering Fundamentals, McGraw-Hill: New York (1986).

Identification and purity of the isolated compound can be assessed by standard techniques in the art. These include high performance liquid chromatography (HPLC), spectroscopy, staining, thin layer chromatography, NIRS, enzymatic analysis, or microbiological methods. The assay method is reviewed in the following literature: Patek et al. (1994) Appl. Environ. Microbiol. 60: 133-140, Malakhova et al. (1996) Biotekhnologiya 11: 27-32, Schmidt et al. (1998) Bioprocess Engineer. 19: 67-70, Ulmann's Encyclopedia of Industrial Chemistry, (1996) vol. A27, VCH: Weinheim, p. 89-90, p.521-540, p.540547, p.559-566, p. 575-581 and p. 581-587, Michal, G. (1999) Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology, John Wiley and Sons, and Falllon, A. et al. (1987) Applications of HPLC in Biochemistry in: Laboratory Techniques in Biochemistry and Molecular Biology, vol. 17.

Example 11: Corey involved in Linkoping neomycin resistance using a Potter-les gene Corynebacterium Cloning of the Glutamicum Gene

A. Identification of genes encoding LMRB proteins

The aceB promoter region of Corynebacterium glutamicum on the polylinker of the lac operon fusion vector pRS415 without promoter (Simon, RW et al. (1987) Gene 53: 85-96) (paceB; Kim, HJ et al. 1997) J. Microbiol. Biotechnol. 7: 287-292) was ligated to prepare plasmid pSL130. This generated paceB-lac region. Plasmid pSL145 was constructed by ligation to the coli cloning vector pACYC184. this. E. coli DH5αF 'was transformed with pSL145 and the resulting strain was used as a host to screen the genome Corynebacterium glutamicum library (library made using pSL109).

Transformants were screened by growing on agar medium containing 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal). White colonies containing DNA that affect lacZ expression were selected and used in other studies. This clone was found to contain 4 kB fragments from the genetic library. Subclones were constructed in pSL109 and the subclones with white phenotype on X-Gal plates were identified. This subclone was found to contain 2.6 kB of BamH1-XhoI fragments (pSL149-5). This fragment was sequenced to identify the membrane protein coding gene (LMRB gene).

1442 nucleotides in the coding sequence of the LMRB gene encode a polypeptide of 481 amino acid residues with a high proportion of hydrophobic amino acids. Genebank search results showed that the LMRB protein was 40% identical to the protein product of the lmrB gene of Bacillus subtilis (Genbank Accession AL009126, TREMBL Accession P94422) when measured using CLUSTALW analysis (using standard parameters).

LMRB protein contains the sequence pattern 158-APALGPTLSG-167 (SEQ ID NO: 301) resembling the known multi-drug-resistant-protein consensus motif GXXXGPXXGG (SEQ ID NO: 302) (Paulsen, IT, and Skurray, RA (1993) Gene 124: 1-11). Therefore, LMRB protein was classified as drug resistant protein.

In vivo analysis of B. lmrB function

As described above, lmrB gene was overexpressed in Corynebacterium glutamicum ASO19E12 (Kim, H. J. et al. (1997) J Microbiol. Biotechnol. 7: 287-292) using plasmid pSL149-5.

The vector pSL18-lmrB was used to disrupt the LMRB gene. This vector was constructed as follows. Internal fragments of the LMRB gene were amplified by PCR under standard conditions using primers 5 'CTCCAGGATTGCTCCGAAGG-3' (SEQ ID NO: 303) and 5 'CACAGTGGTTGACCACTGGC-3' (SEQ ID NO: 304). The resulting PCR product was treated with T7 DNA polymerase and T7 polynucleotide kinase and cloned into the SmaI site of plasmid pSL18 (Kim, YH and H.-S. Lee (1996) J. Microbiol. Biotechnol. 6: 315- 320). The LMRB gene in Corynebacterium glutamicum ASO19E12 was disrupted by conjugation as described above (Schwarzer and Puhler (1991) Bio / Technology 9: 84-87).

Corynebacterium glutamicum cells transformed with pSL149-5 include erythromycin, penicillin G, tetracycline, chloramphenicol, spectinomycin, nalidic acid, gentamicin, streptomycin, ethidium bromide, carbonyl It showed similar resistance to untransformed cells against cyanide m-chlorophenylhydrazone (CCCP) and sodium dodecyl sulfate. However, significant differences were observed in the resistance of transformed and non-transformed cells to lincomycin.

LMRB-overexpressing Corynebacterium glutamicum cells were found to be able to grow in the presence of 20 μg / ml lincomycin. In contrast, cells that did not overexpress LMRB (cells that had destroyed LMRB) could not grow on agar medium containing 5 μg / ml lincomycin. This effect was clearly observed in liquid culture. Overexpression of LMRB increased the resistance to lincomycin nine times (relative to the wild type), and destruction of LMRB reduced the resistance to this antibiotic (reduced by 28% of the wild type).

Example  12: Gene Sequence Analysis of the Invention

Sequence comparison and determination of percent homology between two sequences is a technique known in the art, which is described by Karlin and Altschul (1990) Proc. Natl. Acad. Sci. USA 87: 2264-68, see Karlin and Altschul (1993) Proc. Natl. Acad. Sci. USA 90: 5873-77 may be used using mathematical algorithms, such as modified ones. The algorithm is described in Altschul, et al. (1990) J. Mol. Biol. 215: 403-10, the NBLAST and XBLAST programs (version 2.0). The nucleotide sequence homologous to the SRT nucleic acid molecule of the present invention can be obtained by searching for BLAST nucleotides with the NBLAST program with score = 100, wordlength = 12. The BLAST protein can be searched by the XBLAST program with score = 50 and wordlength = 3 to obtain an amino acid sequence homologous to the SRT protein molecule of the present invention. Altschul et al., (1997) Nucleic Acids Res. 25 (17): 3389-3402, a gap alignment for comparison can be obtained using gap BLAST. Those skilled in the art will know how to optimize the parameters of a program (eg, XBLAST and NBLAST) for the specific sequence to be analyzed when using the BLAST and Gap BLAST programs.

Other examples of mathematical algorithms used for sequence comparisons include Meyers and Miller (1988) Comput. Appl. Biosci. 4: 11-17]. This algorithm is introduced into the ALIGN program (version 2.0) which is part of the GCG sequence alignment software package. When using the ALIGN program for amino acid sequence comparison, the PAM 120 weight residue table, gap length penalty 12, and gap penalty 4 can be used. Additional sequencing algorithms are known in the art and described in Torelli and Robotti (1994) Comput. Appl. Biosci. 10: 3-5] and ADVANCE and ADAM., And Pearson and Lipman (1988) P.N.A.S. 85: 2444-8.

In addition, the percent homology comparison between the two amino acid sequences was performed using the Blosum 62 matrix or PAM250 matrix, and the gap weight 12, 10, 8, 6, or 4 and the length weight 2, 3, or 4 GCG software package. It may also be performed using the GAP program (available at http://www.gcg.com). The percent homology comparison between two nucleic acid sequences can be performed using the GAP program in the GCG software package with standard parameters such as gap weight 50 and length weight 3.

Gene sequences of the present invention were compared to those of Genbank using techniques known in the art (see, eg, Bexevanis and Ouellette, eds. (1998) Bioinformatics: A Practical Guide to the Analysis of Genes and Proteins.John Wiley and Sons: New York. The gene sequence of the present invention and Genebank's gene sequence were compared by a three-step method. In the first step, each of the sequences of the present invention was subjected to BLASTN analysis (eg, local alignment analysis) on the GenBank's nucleotide sequence, leaving the top 500 hits for further analysis. A FASTA search was then performed on the 500 hits (eg, combined local and global alignment analysis, aligning a defined region of the sequence). Then, using the GAP program in the GCG software package (using standard parameters), each gene sequence of the invention was globally aligned to each of the top three FASTA hits. To obtain accurate results, the lengths of sequences selected from GenBank were adjusted to the length of the sequences using methods known in the art. The analysis results are shown in Table 4. The data thus obtained were identical to the data obtained by comparing each gene of the present invention with each of the reference sequences of GenBank by performing GAP (Overall) analysis alone, but when compared with the database-wide GAP (Overall) analysis, Computer use time was much reduced. Unaligned sequences of the present invention are shown in Table 4 as cutoff values without alignment information. Furthermore, those skilled in the art will, in Table 4, list the GAP alignment homology (%) in European numeric format under the heading "% homology (GAP)", where ',' means decimal point. For example, the value "40,345" in this stage means "40.345%".

Example  13: DNA Microarray  Production and implementation

Sequences of the invention are known in the art for the design, methods, and uses of DNA microarrays (DNA arrays), see Schena, M. et al. (1995) Science 270: 467. -470, Wodicka, L. et al. (1997) Nature Biotechnology 15: 1359-1367, DeSaizieu, A. et al. (1998) Nature Biotechnology 16: 45-48, and DeRisi , JL et al. (1997) Science 278: 680-686), and the like.

DNA microarrays are solid or flexible supports composed of nitrocellulose, nylon, glass, silicone, or other materials. Nucleic acid molecules can be attached to this surface in a sequential manner. After appropriate labeling, other nucleic acids or nucleic acid mixtures can be hybridized to the immobilized nucleic acid molecules, and the labels can be used to monitor and measure the individual signal strengths of the hybridized molecules in defined regions. Using this method, it is possible to simultaneously quantify the relative or absolute amounts of all or selected nucleic acids in an applied nucleic acid sample or mixture. Therefore, DNA microarrays allow for simultaneous analysis of large (6800 or more) nucleic acid expression (see, eg, Schena, M. (1996) BioEssays 18 (5): 427-431).

The sequence of the present invention can be used for the design of oligonucleotide primers capable of amplifying a limited region of one or more Corynebacterium glutamicum genes by nucleic acid amplification reactions such as polymerase chain reaction. Selection and design of 5 'or 3' oligonucleotide primers or appropriate linkers resulted in PCR products described above (see also, eg, Schena, M. et al. (1995) Science 270: 467-470). It can also be covalently attached to the surface of the support medium.

In addition, nucleic acid microarrays are described by Wodicka, L. et al. (1997) Nature Biotechnology 15: 1359-1367, may also be prepared by in situ oligonucleotide synthesis. According to the photolithographic method, a matrix of exactly defined areas is exposed to light. As a result, the unstable protecting group is activated to cause nucleotide addition, but no modification occurs to the light-shielded region. Different oligonucleotides are synthesized at positions defined by subsequent protection and photoactivation cycles. Small confined regions of the genes of the invention can be synthesized by solid oligonucleotide synthesis on microarrays.

Nucleic acid molecules of the invention present in a sample or nucleotide mixture can hybridize with the microarray. Such nucleic acid molecules can be labeled according to standard methods. Briefly, isotopic or fluorescently labeled nucleotides are incorporated, for example, during reverse transcription or during DNA synthesis to label nucleic acid molecules (eg, mRNA molecules or DNA molecules). Methods of hybridizing labeled nucleic acids to microarrays are described in, for example, Schena, M. et al. (1995) supra, Dodicka, L. et al. (1997), supra And DeSaizieu A. et al. (1998), supra. Methods of detecting and quantifying hybridized molecules depend on the specific label incorporated. Radiolabels are described, for example, in Schena, M. et al. (1995) supra, and fluorescent labels can be detected, for example, by Shalon et al. (1996) Genome Research 6: 639-645.

As described above, application of the sequences of the present invention to DNA microarray techniques allows for the comparative analysis of different strains of Corynebacterium glutamicum or other Corynebacteria. For example, the study of variants between strains based on individual transcript profiles and the identification of genes important for specific and / or desired strain characteristics, such as pathogenicity, productivity and stress tolerance, are facilitated by nucleic acid alignment. In addition, nucleic acid alignment techniques can be used to compare the gene expression profiles of the present invention during the fermentation reaction process.

Example  14: Intracellular  Protein population kinetics Molecular Analysis (Proteomics)

The genes, compositions, and methods of the present invention can be applied to the interaction and kinetics studies of protein populations, referred to as 'proteomics'. The protein population of interest includes the entire protein population of Corynebacterium glutamicum (eg, as compared to the protein populations of other organisms), specific environmental or metabolic conditions (eg, during fermentation, at high or low temperatures, Or a population of proteins active under high pH or low pH), or a population of proteins active during certain periods of growth and development.

Protein populations can be analyzed by various known techniques, such as gel electrophoresis. Intracellular proteins can be obtained by lysis or extraction and can be separated from one another using a variety of electrophoretic techniques. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separates proteins primarily by their molecular weight. Isoelectric focusing Polyacrylamide gel electrophoresis (IEF-PAGE) separates proteins according to their isoelectric point (meaning not only amino acid sequences but also post-translational variants of the protein). Further preferred protein assays are a continuous parallel of both IEF-PAGE and SDS-PAGE, also known as 2D-gel electrophoresis (see, eg, Hermann et al. (1998) Electrophoresis 19: 3217-3221). , Funtoulakis et al. (1998) Electrophoresis 19: 1193-1202, Langen et al. (1997) Electrophoresis 18: 1184-1192, Antelmann et al. (1997) Electrophoresis 18: 1451-1463 ]. Proteins can also be separated using other separation techniques known in the art, such as capillary gel electrophoresis.

Proteins isolated in this way can be visualized by standard techniques such as staining or labeling. Suitable dyes are known in the art and include fluorescent dyes such as Coomassie Brilliant Blu, Silver Dye, or Sypro Ruby (Molecular Probes) and the like. Radiolabeled amino acids or other protein precursors (eg, ³⁵ S-methionine, ³⁵ S-cysteine, ¹⁴ C-labeled amino acids, ¹⁵ N-amino acids, ¹⁵ NO ₃ or ¹⁵ NH ₄ ⁺ or ¹³ C-labeled Amino acids) can be included in Corynebacterium glutamicum medium to label them before separating proteins from the cells. Similarly, fluorescent labels can be used. The labeled proteins can be extracted, isolated and separated according to the techniques described above.

Proteins visualized by this technique can be further analyzed by measuring the amount of dye or label used. The amount of a given protein can be quantitatively determined using optical methods or the like and can be compared with other protein amounts in the same gel or in other gels. Proteins on the gel can be compared through optical comparison, spectroscopy, image scanning and gel analysis, or the use of photographic films and screens. Such techniques are known in the art.

To determine what any given protein is, direct sequencing or other standard techniques can be used. For example, N- and / or C-terminal amino acid sequencing (e.g., Edman digestion) and mass spectroscopy (especially MALDI or ESI techniques (e.g. Langen et al. (1997)) Electrophoresis 18: 1184-1192). Using these techniques, the protein sequences provided herein can be used to identify Corynebacterium glutamicum proteins.

Using the information obtained in this manner, the presence, activity or modification of the protein between different samples from various biological conditions (e.g., different organisms, time of fermentation, media conditions, or different biotopes) You can compare them. The data obtained alone or in combination with other techniques can be used to compare the reactions of various organisms in a given state (e.g., metabolic), to increase the productivity of microorganisms that produce microchemicals, or to increase the production efficiency of microchemicals. It can be applied for various purposes.

Equivalence

Those skilled in the art will recognize or be able to as well as routine experimental methods, as well as many experimental methods equivalent to certain embodiments of the invention described herein. Such equivalent methods will be included in the following claims.

<110> BASF Aktiengesellschaft <120> CORYNEBACTERIUM GLUTAMICUM GENES ENCODING STRESS,       RESISTANCE AND TOLERANCE PROTEINS <130> BGI-124CPPC <140> PCT / US00 / 00922 <141> 2000-06-23 <150> 60/141031 <151> 1999-06-25 <150> 60/142692 <151> 1999-07-01 <150> 60/151214 <151> 1999-08-27 <150> DE 19930429.7 <151> 1999-07-01 <150> DE 1993 1413.6 <151> 1999-07-08 <150> DE 19931457.8 <151> 1999-07-08 <150> DE 1993 1541.8 <151> 1999-07-08 <150> DE 1993 2209.0 <151> 1999-07-09 <150> DE 19932230.9 <151> 1999-07-09 <150> DE 19932914.1 <151> 1999-07-14 <150> DE 19940764.9 <151> 1999-08-27 <150> DE 1994 1382.7 <151> 1999-08-31 <160> 306 <210> 1 <211> 1566 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1543) <223> RXA01524 <400> 1 ttgtggcact ctttagtagt tttttctcat agctcagttt cgcaacttta gagaactcta 60 gaaactgagc ttcatgctgt gaaaggcctt ttctccattc atg gat tcc caa att 115                                             Met Asp Ser Gln Ile                                               1 5 aat act cag acc tct ccg gca gct gcg aag ctg cct agg gag gtc gtt 163 Asn Thr Gln Thr Ser Pro Ala Ala Ala Lys Leu Pro Arg Glu Val Val                  10 15 20 gtt gtt ctt tcg atc ctc gtg gtt tcc gcg atg atc atg att ctt aat 211 Val Val Leu Ser Ile Leu Val Val Ser Ala Met Ile Met Ile Leu Asn              25 30 35 gaa acc att ctg tcg gtt gcg ttg cct tcc atc atg gaa gat ttc tcc 259 Glu Thr Ile Leu Ser Val Ala Leu Pro Ser Ile Met Glu Asp Phe Ser          40 45 50 gtg cct gaa act act gca cag tgg ttg acc act ggc ttt atg ttg acg 307 Val Pro Glu Thr Thr Ala Gln Trp Leu Thr Thr Gly Phe Met Leu Thr      55 60 65 atg gca gtg gtg att cca act act ggt tat ctg ctt gat cgt ttt tcc 355 Met Ala Val Val Ile Pro Thr Thr Gly Tyr Leu Leu Asp Arg Phe Ser  70 75 80 85 act aag acg atc ttt gtt act gcg ttg ttg ttc ttt acg gtt ggt acg 403 Thr Lys Thr Ile Phe Val Thr Ala Leu Leu Phe Phe Thr Val Gly Thr                  90 95 100 ttg act gcg gcg ttg gct cca acg ttt gcg gtg ctg ctt ggt gct cgt 451 Leu Thr Ala Ala Leu Ala Pro Thr Phe Ala Val Leu Leu Gly Ala Arg             105 110 115 atc gtt cag gcg gtt ggt act gcg ctg gtg atg cct ttg ctg atg acg 499 Ile Val Gln Ala Val Gly Thr Ala Leu Val Met Pro Leu Leu Met Thr         120 125 130 gtt acg ttg acg gtg gtt cct gcg gag cgt cgt ggt tcg atg atg ggc 547 Val Thr Leu Thr Val Val Pro Ala Glu Arg Arg Gly Ser Met Met Gly     135 140 145 att att tcc atc gtg att tct gtt gcg ccg gcg ctt ggt cct acg ttg 595 Ile Ile Ser Ile Val Ile Ser Val Ala Pro Ala Leu Gly Pro Thr Leu 150 155 160 165 tct ggt gtc att ctt aac tct ttg acc tgg cac tgg ttg ttt tgg atg 643 Ser Gly Val Ile Leu Asn Ser Leu Thr Trp His Trp Leu Phe Trp Met                 170 175 180 atg ctt ccg atc gtt gtt atc gct ttg gta att ggt ttc ttc ttg atc 691 Met Leu Pro Ile Val Val Ile Ala Leu Val Ile Gly Phe Phe Leu Ile             185 190 195 aaa aat atc ggc gaa acc aag atc acc cca ctg gat gtt ctg tct gtc 739 Lys Asn Ile Gly Glu Thr Lys Ile Thr Pro Leu Asp Val Leu Ser Val         200 205 210 atc ctt tcg gtg ttt gcc ttc ggt ggt ttg gtg tac ggc ttc agt tcc 787 Ile Leu Ser Val Phe Ala Phe Gly Gly Leu Val Tyr Gly Phe Ser Ser     215 220 225 ttc gga gca atc ctg gag ggc gaa ggc acc gta ggt atc ttc gcg atc 835 Phe Gly Ala Ile Leu Glu Gly Glu Gly Thr Val Gly Ile Phe Ala Ile 230 235 240 245 gtc gtt ggc gcc atc gca ctc ctc atc ttt gct ttg cga cag cac caa 883 Val Val Gly Ala Ile Ala Leu Leu Ile Phe Ala Leu Arg Gln His Gln                 250 255 260 ctc ggc aag caa gac aaa gca ctg atg gat ctc cga gcc ttc aag gtg 931 Leu Gly Lys Gln Asp Lys Ala Leu Met Asp Leu Arg Ala Phe Lys Val             265 270 275 agg aac ttc agc ttc tcc ttg acc acc atc ctt ttg gcg ttc ggc gcg 979 Arg Asn Phe Ser Phe Ser Leu Thr Thr Ile Leu Leu Ala Phe Gly Ala         280 285 290 atg ctc gga acc gtc atg gtt ttg cca atc tac ctg cag act tcc ctc 1027 Met Leu Gly Thr Val Met Val Leu Pro Ile Tyr Leu Gln Thr Ser Leu     295 300 305 gga gtt act gct ttg gtg acc ggt ttg gtt gtt atg ccc ggc ggc ctc 1075 Gly Val Thr Ala Leu Val Thr Gly Leu Val Val Met Pro Gly Gly Leu 310 315 320 325 ctc cag ggt ctg atc agc cca ttc atc gga cgt ttc tac gac aag gtc 1123 Leu Gln Gly Leu Ile Ser Pro Phe Ile Gly Arg Phe Tyr Asp Lys Val                 330 335 340 ggt cca cgt ccg ctg ctg att ccc gga gca att gcg ctg gct atc gcg 1171 Gly Pro Arg Pro Leu Leu Ile Pro Gly Ala Ile Ala Leu Ala Ile Ala             345 350 355 gca tcc tcg atg act ttt ctc aat gag aat tca ccc gtg tgg atg gtc 1219 Ala Ser Ser Met Thr Phe Leu Asn Glu Asn Ser Pro Val Trp Met Val         360 365 370 gtg gtc atg cac gtt gtg ttc agc atc ggc atg tgt ttg atg atg acc 1267 Val Val Met His Val Val Phe Ser Ile Gly Met Cys Leu Met Met Thr     375 380 385 cct ctc atg acc acc gct ctc ggc gcc ctt ccg aag cac ctc tat ggt 1315 Pro Leu Met Thr Thr Ala Leu Gly Ala Leu Pro Lys His Leu Tyr Gly 390 395 400 405 cac ggc tcc gca att ttg aac acg ttc caa cag ctc gca ggc gca gcc 1363 His Gly Ser Ala Ile Leu Asn Thr Phe Gln Gln Leu Ala Gly Ala Ala                 410 415 420 gga aca gcg atc atg att gca gca ctt tcc ttc ggc act tcc att gca 1411 Gly Thr Ala Ile Met Ile Ala Ala Leu Ser Phe Gly Thr Ser Ile Ala             425 430 435 gcg tct tcg gga tct gcg cat gct gaa gct gtt gcc gct ggt acc aag 1459 Ala Ser Ser Gly Ser Ala His Ala Glu Ala Val Ala Ala Gly Thr Lys         440 445 450 gtt gcg ttc atc gca ggc gca atc atc gcg gtg atc gct ttg gtt gtt 1507 Val Ala Phe Ile Ala Gly Ala Ile Ile Ala Val Ile Ala Leu Val Val     455 460 465 tcc ctc ttc gtc act cgc gtc gag gaa gaa gct cac taaataccaa 1553 Ser Leu Phe Val Thr Arg Val Glu Glu Glu Ala His 470 475 480 aaaatggggc aga 1566 <210> 2 <211> 481 <212> PRT <213> Corynebacterium glutamicum <400> 2 Met Asp Ser Gln Ile Asn Thr Gln Thr Ser Pro Ala Ala Ala Lys Leu   1 5 10 15 Pro Arg Glu Val Val Val Val Leu Ser Ile Leu Val Val Ser Ala Met              20 25 30 Ile Met Ile Leu Asn Glu Thr Ile Leu Ser Val Ala Leu Pro Ser Ile          35 40 45 Met Glu Asp Phe Ser Val Pro Glu Thr Thr Ala Gln Trp Leu Thr Thr      50 55 60 Gly Phe Met Leu Thr Met Ala Val Val Ile Pro Thr Thr Gly Tyr Leu  65 70 75 80 Leu Asp Arg Phe Ser Thr Lys Thr Ile Phe Val Thr Ala Leu Leu Phe                  85 90 95 Phe Thr Val Gly Thr Leu Thr Ala Ala Leu Ala Pro Thr Phe Ala Val             100 105 110 Leu Leu Gly Ala Arg Ile Val Gln Ala Val Gly Thr Ala Leu Val Met         115 120 125 Pro Leu Leu Met Thr Val Thr Leu Thr Val Val Pro Ala Glu Arg Arg     130 135 140 Gly Ser Met Met Gly Ile Ile Ser Ile Val Ile Ser Val Ala Pro Ala 145 150 155 160 Leu Gly Pro Thr Leu Ser Gly Val Ile Leu Asn Ser Leu Thr Trp His                 165 170 175 Trp Leu Phe Trp Met Met Leu Pro Ile Val Val Ile Ala Leu Val Ile             180 185 190 Gly Phe Phe Leu Ile Lys Asn Ile Gly Glu Thr Lys Ile Thr Pro Leu         195 200 205 Asp Val Leu Ser Val Ile Leu Ser Val Phe Ala Phe Gly Gly Leu Val     210 215 220 Tyr Gly Phe Ser Ser Phe Gly Ala Ile Leu Glu Gly Glu Gly Thr Val 225 230 235 240 Gly Ile Phe Ala Ile Val Val Gly Ala Ile Ala Leu Leu Ile Phe Ala                 245 250 255 Leu Arg Gln His Gln Leu Gly Lys Gln Asp Lys Ala Leu Met Asp Leu             260 265 270 Arg Ala Phe Lys Val Arg Asn Phe Ser Phe Ser Leu Thr Thr Ile Leu         275 280 285 Leu Ala Phe Gly Ala Met Leu Gly Thr Val Met Val Leu Pro Ile Tyr     290 295 300 Leu Gln Thr Ser Leu Gly Val Thr Ala Leu Val Thr Gly Leu Val Val 305 310 315 320 Met Pro Gly Gly Leu Leu Gln Gly Leu Ile Ser Pro Phe Ile Gly Arg                 325 330 335 Phe Tyr Asp Lys Val Gly Pro Arg Pro Leu Leu Ile Pro Gly Ala Ile             340 345 350 Ala Leu Ala Ile Ala Ala Ser Ser Met Thr Phe Leu Asn Glu Asn Ser         355 360 365 Pro Val Trp Met Val Val Val Met His Val Val Phe Ser Ile Gly Met     370 375 380 Cys Leu Met Met Thr Pro Leu Met Thr Thr Ala Leu Gly Ala Leu Pro 385 390 395 400 Lys His Leu Tyr Gly His Gly Ser Ala Ile Leu Asn Thr Phe Gln Gln                 405 410 415 Leu Ala Gly Ala Ala Gly Thr Ala Ile Met Ile Ala Ala Leu Ser Phe             420 425 430 Gly Thr Ser Ile Ala Ala Ser Ser Gly Ser Ala His Ala Glu Ala Val         435 440 445 Ala Ala Gly Thr Lys Val Ala Phe Ile Ala Gly Ala Ile Ile Ala Val     450 455 460 Ile Ala Leu Val Val Ser Leu Phe Val Thr Arg Val Glu Glu Glu Ala 465 470 475 480 His      <210> 3 <211> 371 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (52) .. (348) <223> RXA00497 <400> 3 tggaaaccca caaccggcac acacaaaatt tttctcatgg agggattcac c gtg gca 57                                                          Val ala                                                           One aac gtc aac atc aag ccg ctt gag gac aag atc ctc gtt cag atc aac 105 Asn Val Asn Ile Lys Pro Leu Glu Asp Lys Ile Leu Val Gln Ile Asn           5 10 15 gaa gca gag acc acc acc gct tcc ggc ctg gtc att cca gat tcc gct 153 Glu Ala Glu Thr Thr Thr Ala Ser Gly Leu Val Ile Pro Asp Ser Ala      20 25 30 aag gaa aag cca caa gag gca acc gtt atc gca gtt ggc cca ggc cgc 201 Lys Glu Lys Pro Gln Glu Ala Thr Val Ile Ala Val Gly Pro Gly Arg  35 40 45 50 ttc gat gac aag ggt aac cgc atc cca ctg gac atc aag gaa gat gac 249 Phe Asp Asp Lys Gly Asn Arg Ile Pro Leu Asp Ile Lys Glu Asp Asp                  55 60 65 gtt gtg atc ttc tcc cgt tac ggc ggc acc gag atc aag ttc ggt ggc 297 Val Val Ile Phe Ser Arg Tyr Gly Gly Thr Glu Ile Lys Phe Gly Gly              70 75 80 gtg gag tac ttg ctt ctc tcc gct cgt gac atc ctc gca atc gtc gag 345 Val Glu Tyr Leu Leu Leu Ser Ala Arg Asp Ile Leu Ala Ile Val Glu          85 90 95 aag taggggataa gttcatggca aag 371 Lys      <210> 4 <211> 99 <212> PRT <213> Corynebacterium glutamicum <400> 4 Val Ala Asn Val Asn Ile Lys Pro Leu Glu Asp Lys Ile Leu Val Gln   1 5 10 15 Ile Asn Glu Ala Glu Thr Thr Thr Ala Ser Gly Leu Val Ile Pro Asp              20 25 30 Ser Ala Lys Glu Lys Pro Gln Glu Ala Thr Val Ile Ala Val Gly Pro          35 40 45 Gly Arg Phe Asp Asp Lys Gly Asn Arg Ile Pro Leu Asp Ile Lys Glu      50 55 60 Asp Asp Val Val Ile Phe Ser Arg Tyr Gly Gly Thr Glu Ile Lys Phe  65 70 75 80 Gly Gly Val Glu Tyr Leu Leu Leu Ser Ala Arg Asp Ile Leu Ala Ile                  85 90 95 Val Glu Lys              <210> 5 <211> 1737 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1714) <223> RXN00493 <400> 5 cccgttacgg cggcaccgag atcaagttcg gtggcgtgga gtacttgctt ctctccgctc 60 gtgacatcct cgcaatcgtc gagaagtagg ggataagttc atg gca aag ctc att 115                                             Met Ala Lys Leu Ile                                               1 5 gct ttt gac cag gac gcc cgc gaa ggc att ctc cgg ggc gtt gac gct 163 Ala Phe Asp Gln Asp Ala Arg Glu Gly Ile Leu Arg Gly Val Asp Ala                  10 15 20 ctg gca aac gct gtc aag gta acc ctc ggc cca cgc ggc cgt aac gtg 211 Leu Ala Asn Ala Val Lys Val Thr Leu Gly Pro Arg Gly Arg Asn Val              25 30 35 gtt ctt gat aag gca ttc ggc gga cct ctg gtc acc aac gac ggt gtc 259 Val Leu Asp Lys Ala Phe Gly Gly Pro Leu Val Thr Asn Asp Gly Val          40 45 50 acc att gcc cgc gac atc gac ctt gag gat cct ttt gag aac ctc ggt 307 Thr Ile Ala Arg Asp Ile Asp Leu Glu Asp Pro Phe Glu Asn Leu Gly      55 60 65 gcg cag ctg gtg aag tcc gtt gct gtt aag acc aac gac atc gct ggt 355 Ala Gln Leu Val Lys Ser Val Ala Val Lys Thr Asn Asp Ile Ala Gly  70 75 80 85 gac ggc acc acg act gca act ctg ctt gct cag gca ctc att gct gaa 403 Asp Gly Thr Thr Thr Ala Thr Leu Leu Ala Gln Ala Leu Ile Ala Glu                  90 95 100 ggc ctg cgc aac gtt gct gct ggc gca aac cca atg gag ctc aac aag 451 Gly Leu Arg Asn Val Ala Ala Gly Ala Asn Pro Met Glu Leu Asn Lys             105 110 115 ggt att tct gca gct gca gaa aag acc ttg gaa gag ttg aag gca cgc 499 Gly Ile Ser Ala Ala Ala Glu Lys Thr Leu Glu Glu Leu Lys Ala Arg         120 125 130 gca acc gag gtg tct gac acc aag gaa atc gca aac gtc gct acc gtt 547 Ala Thr Glu Val Ser Asp Thr Lys Glu Ile Ala Asn Val Ala Thr Val     135 140 145 tca tcc cgc gat gaa gtt gtc ggc gag atc gtt gct gca gcg atg gaa 595 Ser Ser Arg Asp Glu Val Val Gly Glu Ile Val Ala Ala Ala Met Glu 150 155 160 165 aag gtt ggc aag gac ggt gtc gtc acc gtt gag gag tcc cag tcc atc 643 Lys Val Gly Lys Asp Gly Val Val Thr Val Glu Glu Ser Gln Ser Ile                 170 175 180 gag act gct ctc gag gtc acc gaa ggt att tct ttc gac aag ggc tac 691 Glu Thr Ala Leu Glu Val Thr Glu Gly Ile Ser Phe Asp Lys Gly Tyr             185 190 195 ctt tcc cct tat ttc atc aac gac aac gac act cag cag gct gtc ctg 739 Leu Ser Pro Tyr Phe Ile Asn Asp Asn Asp Thr Gln Gln Ala Val Leu         200 205 210 gac aac cct gca gtg ctg ctt gtt cgc aac aag att tct tcc ctc cca 787 Asp Asn Pro Ala Val Leu Leu Val Arg Asn Lys Ile Ser Ser Leu Pro     215 220 225 gac ttc ctc cca ttg ctg gag aag gtt gtg gag tcc aac cgt cct ttg 835 Asp Phe Leu Pro Leu Leu Glu Lys Val Val Glu Ser Asn Arg Pro Leu 230 235 240 245 ctg atc atc gca gaa gac gtc gag ggc gag cct ttg cag acc ctg gtt 883 Leu Ile Ile Ala Glu Asp Val Glu Gly Glu Pro Leu Gln Thr Leu Val                 250 255 260 gtg aac tcc atc cgc aag acc atc aag gtc gtt gca gtg aag tcc cct 931 Val Asn Ser Ile Arg Lys Thr Ile Lys Val Val Ala Val Lys Ser Pro             265 270 275 tac ttc ggt gac cga cgc aag gcg ttc atg gat gac ctg gct att gtc 979 Tyr Phe Gly Asp Arg Arg Lys Ala Phe Met Asp Asp Leu Ala Ile Val         280 285 290 acc aag gca act gtc gtg gat cca gaa gtg ggc atc aac ctc aac gaa 1027 Thr Lys Ala Thr Val Val Asp Pro Glu Val Gly Ile Asn Leu Asn Glu     295 300 305 gct ggc gaa gaa gtt ttc ggt acc gca cgc cgc atc acc gtt tcc aag 1075 Ala Gly Glu Glu Val Phe Gly Thr Ala Arg Arg Ile Thr Val Ser Lys 310 315 320 325 gac gaa acc atc atc gtt gat ggt gca ggt tcc gca gaa gac gtt gaa 1123 Asp Glu Thr Ile Ile Val Asp Gly Ala Gly Ser Ala Glu Asp Val Glu                 330 335 340 gca cgt cgc ggc cag atc cgt cgc gaa atc gcc aac acc gat tcc acc 1171 Ala Arg Arg Gly Gln Ile Arg Arg Glu Ile Ala Asn Thr Asp Ser Thr             345 350 355 tgg gat cgc gaa aag gca gaa gag cgt ttg gct aag ctc tcc ggt ggt 1219 Trp Asp Arg Glu Lys Ala Glu Glu Arg Leu Ala Lys Leu Ser Gly Gly         360 365 370 att gct gtc atc cgc gtt ggt gca gca act gaa acc gaa gtc aac gac 1267 Ile Ala Val Ile Arg Val Gly Ala Ala Thr Glu Thr Glu Val Asn Asp     375 380 385 cgc aag ctg cgt gtc gaa gat gcc atc aac gct gct cgc gca gca gca 1315 Arg Lys Leu Arg Val Glu Asp Ala Ile Asn Ala Ala Arg Ala Ala Ala 390 395 400 405 caa gaa ggc gtt atc gct ggt ggc ggt tcc gct ttg gtt cag atc gct 1363 Gln Glu Gly Val Ile Ala Gly Gly Gly Ser Ala Leu Val Gln Ile Ala                 410 415 420 gag act ctg aag gct tac gcc gaa gag ttc gaa ggc gac cag aag gtc 1411 Glu Thr Leu Lys Ala Tyr Ala Glu Glu Phe Glu Gly Asp Gln Lys Val             425 430 435 ggc gtt cgc gca ctg gct act gct ttg ggc aag cca gcg tac tgg atc 1459 Gly Val Arg Ala Leu Ala Thr Ala Leu Gly Lys Pro Ala Tyr Trp Ile         440 445 450 gcc tcc aac gca ggt ctt gac ggc tct gtt gtt gtt gca cgc act gct 1507 Ala Ser Asn Ala Gly Leu Asp Gly Ser Val Val Val Ala Arg Thr Ala     455 460 465 gct ctg cca aac ggc gag ggc ttc aac gct gca act ttg gaa tac gga 1555 Ala Leu Pro Asn Gly Glu Gly Phe Asn Ala Ala Thr Leu Glu Tyr Gly 470 475 480 485 aac ctg atc aac gac ggt gtc atc gac cca gtc aag gtc acc cat tcc 1603 Asn Leu Ile Asn Asp Gly Val Ile Asp Pro Val Lys Val Thr His Ser                 490 495 500 gca gta gtg aat gca acc tct gtt gca cgc atg gtt ctg acc act gag 1651 Ala Val Val Asn Ala Thr Ser Val Ala Arg Met Val Leu Thr Thr Glu             505 510 515 gct tct gtt gtt gag aag cct gca gaa gaa gca gcc gat gca cat gca 1699 Ala Ser Val Val Glu Lys Pro Ala Glu Glu Ala Ala Asp Ala His Ala         520 525 530 gga cat cat cac cac taaagttctg tgaaaaacac cgt 1737 Gly His His His His     535 <210> 6 <211> 538 <212> PRT <213> Corynebacterium glutamicum <400> 6 Met Ala Lys Leu Ile Ala Phe Asp Gln Asp Ala Arg Glu Gly Ile Leu   1 5 10 15 Arg Gly Val Asp Ala Leu Ala Asn Ala Val Lys Val Thr Leu Gly Pro              20 25 30 Arg Gly Arg Asn Val Val Leu Asp Lys Ala Phe Gly Gly Pro Leu Val          35 40 45 Thr Asn Asp Gly Val Thr Ile Ala Arg Asp Ile Asp Leu Glu Asp Pro      50 55 60 Phe Glu Asn Leu Gly Ala Gln Leu Val Lys Ser Val Ala Val Lys Thr  65 70 75 80 Asn Asp Ile Ala Gly Asp Gly Thr Thr Thr Ala Thr Leu Leu Ala Gln                  85 90 95 Ala Leu Ile Ala Glu Gly Leu Arg Asn Val Ala Ala Gly Ala Asn Pro             100 105 110 Met Glu Leu Asn Lys Gly Ile Ser Ala Ala Ala Glu Lys Thr Leu Glu         115 120 125 Glu Leu Lys Ala Arg Ala Thr Glu Val Ser Asp Thr Lys Glu Ile Ala     130 135 140 Asn Val Ala Thr Val Ser Ser Arg Asp Glu Val Val Gly Glu Ile Val 145 150 155 160 Ala Ala Ala Met Glu Lys Val Gly Lys Asp Gly Val Val Thr Val Glu                 165 170 175 Glu Ser Gln Ser Ile Glu Thr Ala Leu Glu Val Thr Glu Gly Ile Ser             180 185 190 Phe Asp Lys Gly Tyr Leu Ser Pro Tyr Phe Ile Asn Asp Asn Asp Thr         195 200 205 Gln Gln Ala Val Leu Asp Asn Pro Ala Val Leu Leu Val Arg Asn Lys     210 215 220 Ile Ser Ser Leu Pro Asp Phe Leu Pro Leu Leu Glu Lys Val Val Glu 225 230 235 240 Ser Asn Arg Pro Leu Leu Ile Ile Ala Glu Asp Val Glu Gly Glu Pro                 245 250 255 Leu Gln Thr Leu Val Val Asn Ser Ile Arg Lys Thr Ile Lys Val Val             260 265 270 Ala Val Lys Ser Pro Tyr Phe Gly Asp Arg Arg Lys Ala Phe Met Asp         275 280 285 Asp Leu Ala Ile Val Thr Lys Ala Thr Val Val Asp Pro Glu Val Gly     290 295 300 Ile Asn Leu Asn Glu Ala Gly Glu Glu Val Phe Gly Thr Ala Arg Arg 305 310 315 320 Ile Thr Val Ser Lys Asp Glu Thr Ile Ile Val Asp Gly Ala Gly Ser                 325 330 335 Ala Glu Asp Val Glu Ala Arg Arg Gly Gln Ile Arg Arg Glu Ile Ala             340 345 350 Asn Thr Asp Ser Thr Trp Asp Arg Glu Lys Ala Glu Glu Arg Leu Ala         355 360 365 Lys Leu Ser Gly Gly Ile Ala Val Ile Arg Val Gly Ala Ala Thr Glu     370 375 380 Thr Glu Val Asn Asp Arg Lys Leu Arg Val Glu Asp Ala Ile Asn Ala 385 390 395 400 Ala Arg Ala Ala Ala Gln Glu Gly Val Ile Ala Gly Gly Gly Ser Ala                 405 410 415 Leu Val Gln Ile Ala Glu Thr Leu Lys Ala Tyr Ala Glu Glu Phe Glu             420 425 430 Gly Asp Gln Lys Val Gly Val Arg Ala Leu Ala Thr Ala Leu Gly Lys         435 440 445 Pro Ala Tyr Trp Ile Ala Ser Asn Ala Gly Leu Asp Gly Ser Val Val     450 455 460 Val Ala Arg Thr Ala Ala Leu Pro Asn Gly Glu Gly Phe Asn Ala Ala 465 470 475 480 Thr Leu Glu Tyr Gly Asn Leu Ile Asn Asp Gly Val Ile Asp Pro Val                 485 490 495 Lys Val Thr His Ser Ala Val Val Asn Ala Thr Ser Val Ala Arg Met             500 505 510 Val Leu Thr Thr Glu Ala Ser Val Val Glu Lys Pro Ala Glu Glu Ala         515 520 525 Ala Asp Ala His Ala Gly His His His His     530 535 <210> 7 <211> 1339 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (1339) <223> FRXA00498 <400> 7 cccgttacgg cggcaccgag atcaagttcg gtggcgtgga gtacttgctt ctctccgctc 60 gtgacatcct cgcaatcgtc gagaagtagg ggataagttc atg gca aag ctc att 115                                             Met Ala Lys Leu Ile                                               1 5 gct ttt gac cag gac gcc cgc gaa ggc att ctc cgg ggc gtt gac gct 163 Ala Phe Asp Gln Asp Ala Arg Glu Gly Ile Leu Arg Gly Val Asp Ala                  10 15 20 ctg gca aac gct gtc aag gta acc ctc ggc cca cgc ggc cgt aac gtg 211 Leu Ala Asn Ala Val Lys Val Thr Leu Gly Pro Arg Gly Arg Asn Val              25 30 35 gtt ctt gat aag gca ttc ggc gga cct ctg gtc acc aac gac ggt gtc 259 Val Leu Asp Lys Ala Phe Gly Gly Pro Leu Val Thr Asn Asp Gly Val          40 45 50 acc att gcc cgc gac atc gac ctt gag gat cct ttt gag aac ctc ggt 307 Thr Ile Ala Arg Asp Ile Asp Leu Glu Asp Pro Phe Glu Asn Leu Gly      55 60 65 gcg cag ctg gtg aag tcc gtt gct gtt aag acc aac gac atc gct ggt 355 Ala Gln Leu Val Lys Ser Val Ala Val Lys Thr Asn Asp Ile Ala Gly  70 75 80 85 gac ggc acc acg act gca act ctg ctt gct cag gca ctc att gct gaa 403 Asp Gly Thr Thr Thr Ala Thr Leu Leu Ala Gln Ala Leu Ile Ala Glu                  90 95 100 ggc ctg cgc aac gtt gct gct ggc gca aac cca atg gag ctc aac aag 451 Gly Leu Arg Asn Val Ala Ala Gly Ala Asn Pro Met Glu Leu Asn Lys             105 110 115 ggt att tct gca gct gca gaa aag acc ttg gaa gag ttg aag gca cgc 499 Gly Ile Ser Ala Ala Ala Glu Lys Thr Leu Glu Glu Leu Lys Ala Arg         120 125 130 gca acc gag gtg tct gac acc aag gaa atc gca aac gtc gct acc gtt 547 Ala Thr Glu Val Ser Asp Thr Lys Glu Ile Ala Asn Val Ala Thr Val     135 140 145 tca tcc cgc gat gaa gtt gtc ggc gag atc gtt gct gca gcg atg gaa 595 Ser Ser Arg Asp Glu Val Val Gly Glu Ile Val Ala Ala Ala Met Glu 150 155 160 165 aag gtt ggc aag gac ggt gtc gtc acc gtt gag gag tcc cag tcc atc 643 Lys Val Gly Lys Asp Gly Val Val Thr Val Glu Glu Ser Gln Ser Ile                 170 175 180 gag act gct ctc gag gtc acc gaa ggt att tct ttc gac aag ggc tac 691 Glu Thr Ala Leu Glu Val Thr Glu Gly Ile Ser Phe Asp Lys Gly Tyr             185 190 195 ctt tcc cct tat ttc atc aac gac aac gac act cag cag gct gtc ctg 739 Leu Ser Pro Tyr Phe Ile Asn Asp Asn Asp Thr Gln Gln Ala Val Leu         200 205 210 gac aac cct gca gtg ctg ctt gtt cgc aac aag att tct tcc ctc cca 787 Asp Asn Pro Ala Val Leu Leu Val Arg Asn Lys Ile Ser Ser Leu Pro     215 220 225 gac ttc ctc cca ttg ctg gag aag gtt gtg gag tcc aac cgt cct ttg 835 Asp Phe Leu Pro Leu Leu Glu Lys Val Val Glu Ser Asn Arg Pro Leu 230 235 240 245 ctg atc atc gca gaa gac gtc gag ggc gag cct ttg cag acc ctg gtt 883 Leu Ile Ile Ala Glu Asp Val Glu Gly Glu Pro Leu Gln Thr Leu Val                 250 255 260 gtg aac tcc atc cgc aag acc atc aag gtc gtt gca gtg aag tcc cct 931 Val Asn Ser Ile Arg Lys Thr Ile Lys Val Val Ala Val Lys Ser Pro             265 270 275 tac ttc ggt gac cga cgc aag gcg ttc atg gat gac ctg gct att gtc 979 Tyr Phe Gly Asp Arg Arg Lys Ala Phe Met Asp Asp Leu Ala Ile Val         280 285 290 acc aag gca act gtc gtg gat cca gaa gtg ggc atc aac ctc aac gaa 1027 Thr Lys Ala Thr Val Val Asp Pro Glu Val Gly Ile Asn Leu Asn Glu     295 300 305 gct ggc gaa gaa gtt ttc ggt acc gca cgc cgc atc acc gtt tcc aag 1075 Ala Gly Glu Glu Val Phe Gly Thr Ala Arg Arg Ile Thr Val Ser Lys 310 315 320 325 gac gaa acc atc atc gtt gat ggt gca ggt tcc gca gaa gac gtt gaa 1123 Asp Glu Thr Ile Ile Val Asp Gly Ala Gly Ser Ala Glu Asp Val Glu                 330 335 340 gca cgt cgc ggc cag atc cgt cgc gaa atc gcc aac acc gat tcc acc 1171 Ala Arg Arg Gly Gln Ile Arg Arg Glu Ile Ala Asn Thr Asp Ser Thr             345 350 355 tgg gat cgc gaa aag gca gaa gag cgt ttg gct aag ctc tcc ggt ggt 1219 Trp Asp Arg Glu Lys Ala Glu Glu Arg Leu Ala Lys Leu Ser Gly Gly         360 365 370 att gct gtc atc cgc gtt ggt gca gca act gaa acc gaa gtc aac gac 1267 Ile Ala Val Ile Arg Val Gly Ala Ala Thr Glu Thr Glu Val Asn Asp     375 380 385 cgc aag ctg cgt gtc gaa gat gcc atc aac gct gct cgc gca gca gca 1315 Arg Lys Leu Arg Val Glu Asp Ala Ile Asn Ala Ala Arg Ala Ala Ala 390 395 400 405 caa gaa ggc gtt atc gct ggt ggc 1339 Gln Glu Gly Val Ile Ala Gly Gly                 410 <210> 8 <211> 413 <212> PRT <213> Corynebacterium glutamicum <400> 8 Met Ala Lys Leu Ile Ala Phe Asp Gln Asp Ala Arg Glu Gly Ile Leu   1 5 10 15 Arg Gly Val Asp Ala Leu Ala Asn Ala Val Lys Val Thr Leu Gly Pro              20 25 30 Arg Gly Arg Asn Val Val Leu Asp Lys Ala Phe Gly Gly Pro Leu Val          35 40 45 Thr Asn Asp Gly Val Thr Ile Ala Arg Asp Ile Asp Leu Glu Asp Pro      50 55 60 Phe Glu Asn Leu Gly Ala Gln Leu Val Lys Ser Val Ala Val Lys Thr  65 70 75 80 Asn Asp Ile Ala Gly Asp Gly Thr Thr Thr Ala Thr Leu Leu Ala Gln                  85 90 95 Ala Leu Ile Ala Glu Gly Leu Arg Asn Val Ala Ala Gly Ala Asn Pro             100 105 110 Met Glu Leu Asn Lys Gly Ile Ser Ala Ala Ala Glu Lys Thr Leu Glu         115 120 125 Glu Leu Lys Ala Arg Ala Thr Glu Val Ser Asp Thr Lys Glu Ile Ala     130 135 140 Asn Val Ala Thr Val Ser Ser Arg Asp Glu Val Val Gly Glu Ile Val 145 150 155 160 Ala Ala Ala Met Glu Lys Val Gly Lys Asp Gly Val Val Thr Val Glu                 165 170 175 Glu Ser Gln Ser Ile Glu Thr Ala Leu Glu Val Thr Glu Gly Ile Ser             180 185 190 Phe Asp Lys Gly Tyr Leu Ser Pro Tyr Phe Ile Asn Asp Asn Asp Thr         195 200 205 Gln Gln Ala Val Leu Asp Asn Pro Ala Val Leu Leu Val Arg Asn Lys     210 215 220 Ile Ser Ser Leu Pro Asp Phe Leu Pro Leu Leu Glu Lys Val Val Glu 225 230 235 240 Ser Asn Arg Pro Leu Leu Ile Ile Ala Glu Asp Val Glu Gly Glu Pro                 245 250 255 Leu Gln Thr Leu Val Val Asn Ser Ile Arg Lys Thr Ile Lys Val Val             260 265 270 Ala Val Lys Ser Pro Tyr Phe Gly Asp Arg Arg Lys Ala Phe Met Asp         275 280 285 Asp Leu Ala Ile Val Thr Lys Ala Thr Val Val Asp Pro Glu Val Gly     290 295 300 Ile Asn Leu Asn Glu Ala Gly Glu Glu Val Phe Gly Thr Ala Arg Arg 305 310 315 320 Ile Thr Val Ser Lys Asp Glu Thr Ile Ile Val Asp Gly Ala Gly Ser                 325 330 335 Ala Glu Asp Val Glu Ala Arg Arg Gly Gln Ile Arg Arg Glu Ile Ala             340 345 350 Asn Thr Asp Ser Thr Trp Asp Arg Glu Lys Ala Glu Glu Arg Leu Ala         355 360 365 Lys Leu Ser Gly Gly Ile Ala Val Ile Arg Val Gly Ala Ala Thr Glu     370 375 380 Thr Glu Val Asn Asp Arg Lys Leu Arg Val Glu Asp Ala Ile Asn Ala 385 390 395 400 Ala Arg Ala Ala Ala Gln Glu Gly Val Ile Ala Gly Gly                 405 410 <210> 9 <211> 723 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (700) <223> RXA01217 <400> 9 gcgctgcctg cgaagactcg accaagacat tcgagtcggt cgcgggcatt ttttattttc 60 gcggccgagt gtccaccttc atccatgagg agaaatcact atg gca aag tac caa 115                                             Met Ala Lys Tyr Gln                                               1 5 acc att gag gct gct gtc cgc tct gag ttc ggc aag ggc tct gca cgt 163 Thr Ile Glu Ala Ala Val Arg Ser Glu Phe Gly Lys Gly Ser Ala Arg                  10 15 20 cgt gca cgc gtt gct ggc cag atc cct gct gtt gtt tac ggc gca gat 211 Arg Ala Arg Val Ala Gly Gln Ile Pro Ala Val Val Tyr Gly Ala Asp              25 30 35 gtt gag tcc aac ctg cac gtc acc atc gac cac cgc acc ttc gct gcg 259 Val Glu Ser Asn Leu His Val Thr Ile Asp His Arg Thr Phe Ala Ala          40 45 50 ctg gtt cgc cag gaa ggc gta aac gct gtt ctt gag ctc gac atc gag 307 Leu Val Arg Gln Glu Gly Val Asn Ala Val Leu Glu Leu Asp Ile Glu      55 60 65 ggc cag aag cag ctc acc atg atc aag cac atc gac cag aac gtg ctg 355 Gly Gln Lys Gln Leu Thr Met Ile Lys His Ile Asp Gln Asn Val Leu  70 75 80 85 acc ttc cac atc gac cac ttg gac ctg ctt gcc att aag cgc ggc gaa 403 Thr Phe His Ile Asp His Leu Asp Leu Leu Ala Ile Lys Arg Gly Glu                  90 95 100 aag gtt gag gtt gac gtt cca gtt atc gtc gag ggc gag cca gct cca 451 Lys Val Glu Val Asp Val Pro Val Ile Val Glu Gly Glu Pro Ala Pro             105 110 115 ggc acc atg tgg gtt cag gat gct gac acc atc aag gtt gag gct gac 499 Gly Thr Met Trp Val Gln Asp Ala Asp Thr Ile Lys Val Glu Ala Asp         120 125 130 gtt ctg tcc atc cct gaa gag ttc acc gtt tcc atc gaa ggc ctt gag 547 Val Leu Ser Ile Pro Glu Glu Phe Thr Val Ser Ile Glu Gly Leu Glu     135 140 145 ctc ggc gca cag atc acc gca gct gac atc aag ctc gag ggc gac acc 595 Leu Gly Ala Gln Ile Thr Ala Ala Asp Ile Lys Leu Glu Gly Asp Thr 150 155 160 165 acc ctg gtt gag gat cct gag acc ctc atc gtc aac atc gtt ctc cca 643 Thr Leu Val Glu Asp Pro Glu Thr Leu Ile Val Asn Ile Val Leu Pro                 170 175 180 gct gtc gag gaa gaa gac acc gaa gag gac gaa gca gct gaa gaa gca 691 Ala Val Glu Glu Glu Asp Thr Glu Glu Asp Glu Ala Ala Glu Glu Ala             185 190 195 gct act gag taagcttttt tagatagctt tat 723 Ala Thr Glu         200 <210> 10 <211> 200 <212> PRT <213> Corynebacterium glutamicum <400> 10 Met Ala Lys Tyr Gln Thr Ile Glu Ala Ala Val Arg Ser Glu Phe Gly   1 5 10 15 Lys Gly Ser Ala Arg Arg Ala Arg Val Ala Gly Gln Ile Pro Ala Val              20 25 30 Val Tyr Gly Ala Asp Val Glu Ser Asn Leu His Val Thr Ile Asp His          35 40 45 Arg Thr Phe Ala Ala Leu Val Arg Gln Glu Gly Val Asn Ala Val Leu      50 55 60 Glu Leu Asp Ile Glu Gly Gln Lys Gln Leu Thr Met Ile Lys His Ile  65 70 75 80 Asp Gln Asn Val Leu Thr Phe His Ile Asp His Leu Asp Leu Leu Ala                  85 90 95 Ile Lys Arg Gly Glu Lys Val Glu Val Asp Val Pro Val Ile Val Glu             100 105 110 Gly Glu Pro Ala Pro Gly Thr Met Trp Val Gln Asp Ala Asp Thr Ile         115 120 125 Lys Val Glu Ala Asp Val Leu Ser Ile Pro Glu Glu Phe Thr Val Ser     130 135 140 Ile Glu Gly Leu Glu Leu Gly Ala Gln Ile Thr Ala Ala Asp Ile Lys 145 150 155 160 Leu Glu Gly Asp Thr Thr Leu Val Glu Asp Pro Glu Thr Leu Ile Val                 165 170 175 Asn Ile Val Leu Pro Ala Val Glu Glu Glu Asp Thr Glu Glu Asp Glu             180 185 190 Ala Ala Glu Glu Ala Ala Thr Glu         195 200 <210> 11 <211> 1603 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (33) .. (1580) <223> RXA00605 <400> 11 ataggtaacc ctcataaaag gaaggaatgc ta atg tct gag aag tca gca gca 53                                     Met Ser Glu Lys Ser Ala Ala                                      1 5 gac cag atc gta gat cgc gga atg cgt cca aag ctt tct gga aac act 101 Asp Gln Ile Val Asp Arg Gly Met Arg Pro Lys Leu Ser Gly Asn Thr          10 15 20 acc cgc cac aac gga gca cca gtt cca tct gag aac atc tcc gca acc 149 Thr Arg His Asn Gly Ala Pro Val Pro Ser Glu Asn Ile Ser Ala Thr      25 30 35 gca ggc cca cag ggt cca aac gtt ctc aat gac att cac ctc att gaa 197 Ala Gly Pro Gln Gly Pro Asn Val Leu Asn Asp Ile His Leu Ile Glu  40 45 50 55 aag ctc gca cac ttt aac cgc gag aac gtt cca gag cgt atc cct cac 245 Lys Leu Ala His Phe Asn Arg Glu Asn Val Pro Glu Arg Ile Pro His                  60 65 70 gca aag ggc cac ggc gct ttc ggt gag ctg cac atc acc gag gac gta 293 Ala Lys Gly His Gly Ala Phe Gly Glu Leu His Ile Thr Glu Asp Val              75 80 85 tcc gaa tac acc aag gca gac ctg ttc cag cct ggt aag gtc acc ccg 341 Ser Glu Tyr Thr Lys Ala Asp Leu Phe Gln Pro Gly Lys Val Thr Pro          90 95 100 ctg gct gtt cgc ttc tct act gtt gct ggt gag cag ggc tcc cca gat 389 Leu Ala Val Arg Phe Ser Thr Val Ala Gly Glu Gln Gly Ser Pro Asp     105 110 115 acc tgg cgc gac gtc cac ggc ttc gct ctt cgc ttc tac acc gaa gag 437 Thr Trp Arg Asp Val His Gly Phe Ala Leu Arg Phe Tyr Thr Glu Glu 120 125 130 135 ggc aac tac gac atc gtg ggt aac aac acc cca acc ttc ttc ctt cgt 485 Gly Asn Tyr Asp Ile Val Gly Asn Asn Thr Pro Thr Phe Phe Leu Arg                 140 145 150 gac ggc atg aag ttc ccg gac ttc atc cac tca cag aag cgt ctc aac 533 Asp Gly Met Lys Phe Pro Asp Phe Ile His Ser Gln Lys Arg Leu Asn             155 160 165 aag aac ggt ctg cgc gat gca gac atg cag tgg gat ttc tgg acc cgc 581 Lys Asn Gly Leu Arg Asp Ala Asp Met Gln Trp Asp Phe Trp Thr Arg         170 175 180 gca cct gaa tct gca cac cag gtg acc tac ctg atg ggt gac cgc ggt 629 Ala Pro Glu Ser Ala His Gln Val Thr Tyr Leu Met Gly Asp Arg Gly     185 190 195 acc cct aag acc tcc cgc cac cag gac ggc ttc ggc tcc cac acc ttc 677 Thr Pro Lys Thr Ser Arg His Gln Asp Gly Phe Gly Ser His Thr Phe 200 205 210 215 cag tgg atc aac gct gaa ggt aag cca gtt tgg gtt aag tac cac ttc 725 Gln Trp Ile Asn Ala Glu Gly Lys Pro Val Trp Val Lys Tyr His Phe                 220 225 230 aag acc cgc cag ggc tgg gat tgc ttc acc gat gca gaa gca gca aag 773 Lys Thr Arg Gln Gly Trp Asp Cys Phe Thr Asp Ala Glu Ala Ala Lys             235 240 245 gtt gca ggc gag aac gct gac tac cag cgc gaa gac ctc tac aac gct 821 Val Ala Gly Glu Asn Ala Asp Tyr Gln Arg Glu Asp Leu Tyr Asn Ala         250 255 260 att gaa aac ggc gac ttc cca atc tgg gac gtc aag gtt cag atc atg 869 Ile Glu Asn Gly Asp Phe Pro Ile Trp Asp Val Lys Val Gln Ile Met     265 270 275 cct ttc gag gat gca gag aac tac cgc tgg aac cca ttc gac ctg acc 917 Pro Phe Glu Asp Ala Glu Asn Tyr Arg Trp Asn Pro Phe Asp Leu Thr 280 285 290 295 aag acc tgg tcc cag aag gat tac cca ctg atc cca gtc ggt tac ttc 965 Lys Thr Trp Ser Gln Lys Asp Tyr Pro Leu Ile Pro Val Gly Tyr Phe                 300 305 310 atc ctg aac cgc aac cca cgc aac ttc ttc gct cag atc gag cag ctt 1013 Ile Leu Asn Arg Asn Pro Arg Asn Phe Phe Ala Gln Ile Glu Gln Leu             315 320 325 gca ctg gat cca ggc aac atc gtt cct ggc gtc ggc ctg tcc cca gac 1061 Ala Leu Asp Pro Gly Asn Ile Val Pro Gly Val Gly Leu Ser Pro Asp         330 335 340 cgc atg ctc cag gca cgt atc ttc gca tac gct gac cag cag cgt tac 1109 Arg Met Leu Gln Ala Arg Ile Phe Ala Tyr Ala Asp Gln Gln Arg Tyr     345 350 355 cgc atc ggc gct aac tac cgc gac ctg cca gtg aac cgt cca atc aac 1157 Arg Ile Gly Ala Asn Tyr Arg Asp Leu Pro Val Asn Arg Pro Ile Asn 360 365 370 375 gag gtc aac acc tac agc cgc gaa ggt tcc atg cag tac atc ttc gac 1205 Glu Val Asn Thr Tyr Ser Arg Glu Gly Ser Met Gln Tyr Ile Phe Asp                 380 385 390 gct gag ggc gag cct tcc tac agc cct aac cgc tac gac aag ggc gca 1253 Ala Glu Gly Glu Pro Ser Tyr Ser Pro Asn Arg Tyr Asp Lys Gly Ala             395 400 405 ggc tac ctg gat aac ggt acg gat tcc tcc tcc aac cac acc tcc tac 1301 Gly Tyr Leu Asp Asn Gly Thr Asp Ser Ser Ser Asn His Thr Ser Tyr         410 415 420 ggc cag gct gat gac atc tac gtc aac cca gac cca cac ggc acc gac 1349 Gly Gln Ala Asp Asp Ile Tyr Val Asn Pro Asp Pro His Gly Thr Asp     425 430 435 ctg gtt cgt gct gct tac gtc aag cac cag gat gat gac gac ttc atc 1397 Leu Val Arg Ala Ala Tyr Val Lys His Gln Asp Asp Asp Phe Ile 440 445 450 455 cag cca ggc atc cta tac cgc gag gtc ctg gat gag ggc gag aag gag 1445 Gln Pro Gly Ile Leu Tyr Arg Glu Val Leu Asp Glu Gly Glu Lys Glu                 460 465 470 cga ttg gca gac aac atc tcc aac gca atg cag ggc atc tct gag gca 1493 Arg Leu Ala Asp Asn Ile Ser Asn Ala Met Gln Gly Ile Ser Glu Ala             475 480 485 acc gag cca cgc gtc tac gac tac tgg aac aac gtt gat gag aac ctc 1541 Thr Glu Pro Arg Val Tyr Asp Tyr Trp Asn Asn Val Asp Glu Asn Leu         490 495 500 ggc gct cgc gtc aag gag ctt tac ctc cag aag aag gct taagtccttc 1590 Gly Ala Arg Val Lys Glu Leu Tyr Leu Gln Lys Lys Ala     505 510 515 tgatttaaaa tga 1603 <210> 12 <211> 516 <212> PRT <213> Corynebacterium glutamicum <400> 12 Met Ser Glu Lys Ser Ala Ala Asp Gln Ile Val Asp Arg Gly Met Arg   1 5 10 15 Pro Lys Leu Ser Gly Asn Thr Thr Arg His Asn Gly Ala Pro Val Pro              20 25 30 Ser Glu Asn Ile Ser Ala Thr Ala Gly Pro Gln Gly Pro Asn Val Leu          35 40 45 Asn Asp Ile His Leu Ile Glu Lys Leu Ala His Phe Asn Arg Glu Asn      50 55 60 Val Pro Glu Arg Ile Pro His Ala Lys Gly His Gly Ala Phe Gly Glu  65 70 75 80 Leu His Ile Thr Glu Asp Val Ser Glu Tyr Thr Lys Ala Asp Leu Phe                  85 90 95 Gln Pro Gly Lys Val Thr Pro Leu Ala Val Arg Phe Ser Thr Val Ala             100 105 110 Gly Glu Gln Gly Ser Pro Asp Thr Trp Arg Asp Val His Gly Phe Ala         115 120 125 Leu Arg Phe Tyr Thr Glu Glu Gly Asn Tyr Asp Ile Val Gly Asn Asn     130 135 140 Thr Pro Thr Phe Phe Leu Arg Asp Gly Met Lys Phe Pro Asp Phe Ile 145 150 155 160 His Ser Gln Lys Arg Leu Asn Lys Asn Gly Leu Arg Asp Ala Asp Met                 165 170 175 Gln Trp Asp Phe Trp Thr Arg Ala Pro Glu Ser Ala His Gln Val Thr             180 185 190 Tyr Leu Met Gly Asp Arg Gly Thr Pro Lys Thr Ser Arg His Gln Asp         195 200 205 Gly Phe Gly Ser His Thr Phe Gln Trp Ile Asn Ala Glu Gly Lys Pro     210 215 220 Val Trp Val Lys Tyr His Phe Lys Thr Arg Gln Gly Trp Asp Cys Phe 225 230 235 240 Thr Asp Ala Glu Ala Ala Lys Val Ala Gly Glu Asn Ala Asp Tyr Gln                 245 250 255 Arg Glu Asp Leu Tyr Asn Ala Ile Glu Asn Gly Asp Phe Pro Ile Trp             260 265 270 Asp Val Lys Val Gln Ile Met Pro Phe Glu Asp Ala Glu Asn Tyr Arg         275 280 285 Trp Asn Pro Phe Asp Leu Thr Lys Thr Trp Ser Gln Lys Asp Tyr Pro     290 295 300 Leu Ile Pro Val Gly Tyr Phe Ile Leu Asn Arg Asn Pro Arg Asn Phe 305 310 315 320 Phe Ala Gln Ile Glu Gln Leu Ala Leu Asp Pro Gly Asn Ile Val Pro                 325 330 335 Gly Val Gly Leu Ser Pro Asp Arg Met Leu Gln Ala Arg Ile Phe Ala             340 345 350 Tyr Ala Asp Gln Gln Arg Tyr Arg Ile Gly Ala Asn Tyr Arg Asp Leu         355 360 365 Pro Val Asn Arg Pro Ile Asn Glu Val Asn Thr Tyr Ser Arg Glu Gly     370 375 380 Ser Met Gln Tyr Ile Phe Asp Ala Glu Gly Glu Pro Ser Tyr Ser Pro 385 390 395 400 Asn Arg Tyr Asp Lys Gly Ala Gly Tyr Leu Asp Asn Gly Thr Asp Ser                 405 410 415 Ser Ser Asn His Thr Ser Tyr Gly Gln Ala Asp Asp Ile Tyr Val Asn             420 425 430 Pro Asp Pro His Gly Thr Asp Leu Val Arg Ala Ala Tyr Val Lys His         435 440 445 Gln Asp Asp Asp Asp Phe Ile Gln Pro Gly Ile Leu Tyr Arg Glu Val     450 455 460 Leu Asp Glu Gly Glu Lys Glu Arg Leu Ala Asp Asn Ile Ser Asn Ala 465 470 475 480 Met Gln Gly Ile Ser Glu Ala Thr Glu Pro Arg Val Tyr Asp Tyr Trp                 485 490 495 Asn Asn Val Asp Glu Asn Leu Gly Ala Arg Val Lys Glu Leu Tyr Leu             500 505 510 Gln Lys Lys Ala         515 <210> 13 <211> 2439 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (2416) <223> RXA00404 <400> 13 aagatccgat catcggcata cagaaacacc catctggccg aactttcctt tttctgcatg 60 catttctgca cacagtttct gcccgctgtt tctacgcata gtg gct ttg aaa cga 115                                             Val Ala Leu Lys Arg                                               1 5 ccc gaa gag aaa aca gta aag atc gtg acc ata aaa cag act gac aac 163 Pro Glu Glu Lys Thr Val Lys Ile Val Thr Ile Lys Gln Thr Asp Asn                  10 15 20 atc aat gac gat gat ttg gtg tac agc aac gct act gac ctt cca gta 211 Ile Asn Asp Asp Asp Leu Val Tyr Ser Asn Ala Thr Asp Leu Pro Val              25 30 35 ggc gtg aag aag tcc cct aaa atg tca ccg acc gcc cgc gtt ggt ctc 259 Gly Val Lys Lys Ser Pro Lys Met Ser Pro Thr Ala Arg Val Gly Leu          40 45 50 ctt gtc ttt ggg gtt atc gcg gcg gtg ggt tgg gga gca atc gct ttc 307 Leu Val Phe Gly Val Ile Ala Ala Val Gly Trp Gly Ala Ile Ala Phe      55 60 65 tcc cgt ggc gaa aca atc aac tct gtg tgg ctg gtt ttg gcg gca gtt 355 Ser Arg Gly Glu Thr Ile Asn Ser Val Trp Leu Val Leu Ala Ala Val  70 75 80 85 ggt tcc tat atc att gcg ttt tct ttc tat gcc cga ctg att gaa tac 403 Gly Ser Tyr Ile Ile Ala Phe Ser Phe Tyr Ala Arg Leu Ile Glu Tyr                  90 95 100 aaa gtt gtt aag ccg aaa gat cag cga gca acc ccg gcg gaa tac gtt 451 Lys Val Val Lys Pro Lys Asp Gln Arg Ala Thr Pro Ala Glu Tyr Val             105 110 115 aat gac ggc aag gac tat gtc cca acg gat cgt cgt gtg ctt ttt ggc 499 Asn Asp Gly Lys Asp Tyr Val Pro Thr Asp Arg Arg Val Leu Phe Gly         120 125 130 cac cac ttt gca gct att gca ggt gcc ggt cca ttg gtt gga cct gtc 547 His His Phe Ala Ala Ile Ala Gly Ala Gly Pro Leu Val Gly Pro Val     135 140 145 atg gcc gcg cag atg ggc tac ctg cca ggc acc ttg tgg att atc ctc 595 Met Ala Ala Gln Met Gly Tyr Leu Pro Gly Thr Leu Trp Ile Ile Leu 150 155 160 165 ggt gtg att ttc gcc ggt gca gtg cag gac tac cta gtg ctg tgg gtg 643 Gly Val Ile Phe Ala Gly Ala Val Gln Asp Tyr Leu Val Leu Trp Val                 170 175 180 tct act cgt agg cgt gga cgc tca ctt ggc cag atg gtt cgt gat gaa 691 Ser Thr Arg Arg Arg Gly Arg Ser Leu Gly Gln Met Val Arg Asp Glu             185 190 195 atg ggc acg gtc ggt gga gct gcc ggt atc ttg gcg acc atc tcc atc 739 Met Gly Thr Val Gly Gly Ala Ala Gly Ile Leu Ala Thr Ile Ser Ile         200 205 210 atg atc atc att atc gcg gtg ctc gca ttg atc gtg gtt aat gca ctg 787 Met Ile Ile Ile Ile Ala Val Leu Ala Leu Ile Val Val Asn Ala Leu     215 220 225 gct gat tca cca tgg ggc gtt ttc tcc atc acc atg acc atc cca att 835 Ala Asp Ser Pro Trp Gly Val Phe Ser Ile Thr Met Thr Ile Pro Ile 230 235 240 245 gca ctg ttc atg ggt gtg tac ttg cgt tac ctg cgc cca ggt cgt gtt 883 Ala Leu Phe Met Gly Val Tyr Leu Arg Tyr Leu Arg Pro Gly Arg Val                 250 255 260 act gaa gtg tcc atc atc ggt gtg gca ctg ctc ctg ctg gct atc gtt 931 Thr Glu Val Ser Ile Ile Gly Val Ala Leu Leu Leu Leu Ala Ile Val             265 270 275 gct ggt ggt tgg gtt gca gac acc tca tgg ggc gtg gaa tgg ttc acc 979 Ala Gly Gly Trp Val Ala Asp Thr Ser Trp Gly Val Glu Trp Phe Thr         280 285 290 tgg tct aag acc act ttg gcg ttg gcc ttg atc ggt tac gga atc atg 1027 Trp Ser Lys Thr Thr Leu Ala Leu Ala Leu Ile Gly Tyr Gly Ile Met     295 300 305 gct gcg att ttg ccg gtg tgg ctg ctg ctt gca ccg cgc gat tac ctg 1075 Ala Ala Ile Leu Pro Val Trp Leu Leu Leu Ala Pro Arg Asp Tyr Leu 310 315 320 325 tct acc ttt atg aag atc ggc gtc atc ggt ctg ttg gca gtg ggt att 1123 Ser Thr Phe Met Lys Ile Gly Val Ile Gly Leu Leu Ala Val Gly Ile                 330 335 340 ttg ttc gca cgt cct gag gtg cag atg cct tcc gtg acc tcc ttc gca 1171 Leu Phe Ala Arg Pro Glu Val Gln Met Pro Ser Val Thr Ser Phe Ala             345 350 355 ctt gag ggc aac ggt ccg gtg ttc tct gga agt ctg ttc cca ttc ctg 1219 Leu Glu Gly Asn Gly Pro Val Phe Ser Gly Ser Leu Phe Pro Phe Leu         360 365 370 ttc atc acg att gcc tgt ggt gca ctg tct ggt ttc cac gca ctg att 1267 Phe Ile Thr Ile Ala Cys Gly Ala Leu Ser Gly Phe His Ala Leu Ile     375 380 385 tct tca gga acc aca cca aag ctt gtg gag aag gaa tcc cag atg cgc 1315 Ser Ser Gly Thr Thr Pro Lys Leu Val Glu Lys Glu Ser Gln Met Arg 390 395 400 405 atg ctc ggc tac ggc ggc atg ttg atg gaa tct ttc gtg gcg atg atg 1363 Met Leu Gly Tyr Gly Gly Met Leu Met Glu Ser Phe Val Ala Met Met                 410 415 420 gca ctg atc acc gct gtt att ctg gat cgt cac ctg tac ttc tcc atg 1411 Ala Leu Ile Thr Ala Val Ile Leu Asp Arg His Leu Tyr Phe Ser Met             425 430 435 aac gct ccg ctg gca ctg act ggt gga gat cca gca acc gca gct gag 1459 Asn Ala Pro Leu Ala Leu Thr Gly Gly Asp Pro Ala Thr Ala Ala Glu         440 445 450 tgg gtt aac tcc att ggg ctg aca ggt gcg gat atc acc ccg gaa cag 1507 Trp Val Asn Ser Ile Gly Leu Thr Gly Ala Asp Ile Thr Pro Glu Gln     455 460 465 ctg tcg gaa gct gct gaa agt gtc gga gaa tcc act gtt att tcc cgt 1555 Leu Ser Glu Ala Ala Glu Ser Val Gly Glu Ser Thr Val Ile Ser Arg 470 475 480 485 acc ggt ggc gca cca acc ttg gcg ttc ggt atg tct gaa atc ctc tcc 1603 Thr Gly Gly Ala Pro Thr Leu Ala Phe Gly Met Ser Glu Ile Leu Ser                 490 495 500 gga ttc atc ggc ggc gct gga atg aag gcg ttc tgg tac cac ttc gcc 1651 Gly Phe Ile Gly Gly Ala Gly Met Lys Ala Phe Trp Tyr His Phe Ala             505 510 515 atc atg ttt gag gct ctg ttc atc ctc act act gtg gat gca ggt act 1699 Ile Met Phe Glu Ala Leu Phe Ile Leu Thr Thr Val Val Asp Ala Gly Thr         520 525 530 cgt gtg gct cgc ttt atg atg acc gat acc ttg ggc aat gtt cca ggt 1747 Arg Val Ala Arg Phe Met Met Thr Asp Thr Leu Gly Asn Val Pro Gly     535 540 545 ctg cgc cgt ttc aag gat cct tca tgg act gtc ggt aac tgg att tct 1795 Leu Arg Arg Phe Lys Asp Pro Ser Trp Thr Val Gly Asn Trp Ile Ser 550 555 560 565 acc gtg ttt gtg tgt gct cta tgg ggt gct att ttg ctc atg ggt gtt 1843 Thr Val Phe Val Cys Ala Leu Trp Gly Ala Ile Leu Leu Met Gly Val                 570 575 580 acc gat cca ctg ggc ggc atc aac gtg ctt ttc cca cta ttc ggt atc 1891 Thr Asp Pro Leu Gly Gly Ile Asn Val Leu Phe Pro Leu Phe Gly Ile             585 590 595 gct aac cag ctg ctc gcc gct att gca ctt gct ctc gtg ctg gtt gtt 1939 Ala Asn Gln Leu Leu Ala Ala Ile Ala Leu Ala Leu Val Leu Val Val         600 605 610 gtg gtg aag aag ggc ctg tac aag tgg gcg tgg att cca gct gtt cct 1987 Val Val Lys Lys Gly Leu Tyr Lys Trp Ala Trp Ile Pro Ala Val Pro     615 620 625 ttg gca tgg gat ctc att gtc acg atg act gcg tca tgg cag aag att 2035 Leu Ala Trp Asp Leu Ile Val Thr Met Thr Ala Ser Trp Gln Lys Ile 630 635 640 645 ttc cac tct gat ccg gct att ggc tac tgg gct cag aac gcg aac ttc 2083 Phe His Ser Asp Pro Ala Ile Gly Tyr Trp Ala Gln Asn Ala Asn Phe                 650 655 660 cgc gat gca aag tct caa ggc ctt acc gaa ttt ggt gcc gct aaa tct 2131 Arg Asp Ala Lys Ser Gln Gly Leu Thr Glu Phe Gly Ala Ala Lys Ser             665 670 675 cct gag gca atc gat gcg gtt atc cga aac acc atg att cag ggc atc 2179 Pro Glu Ala Ile Asp Ala Val Ile Arg Asn Thr Met Ile Gln Gly Ile         680 685 690 ttg tcc atc ctg ttc gcg gtg ctc gtc ctc gtt gtt gtc ggc gca gcc 2227 Leu Ser Ile Leu Phe Ala Val Leu Val Leu Val Val Val Gly Ala Ala     695 700 705 att gcg gtg tgc atc aag tcc atc agg gct cgt gca gcc gga aca cct 2275 Ile Ala Val Cys Ile Lys Ser Ile Arg Ala Arg Ala Ala Gly Thr Pro 710 715 720 725 ttg gag acc act gaa gag cct gat act gaa tct gag ttc ttc gcc cca 2323 Leu Glu Thr Thr Glu Glu Pro Asp Thr Glu Ser Glu Phe Phe Ala Pro                 730 735 740 act gga ttc ctt gca tct tcc agg gat aag gaa gtc cag gcc atg tgg 2371 Thr Gly Phe Leu Ala Ser Ser Arg Asp Lys Glu Val Gln Ala Met Trp             745 750 755 gac gag cgc tac cca ggc ggt gcg ccc gtg tct tct gga ggg cac 2416 Asp Glu Arg Tyr Pro Gly Gly Ala Pro Val Ser Ser Gly Gly His         760 765 770 taaaacatga tggctcttac tca 2439 <210> 14 <211> 772 <212> PRT <213> Corynebacterium glutamicum <400> 14 Val Ala Leu Lys Arg Pro Glu Glu Lys Thr Val Lys Ile Val Thr Ile   1 5 10 15 Lys Gln Thr Asp Asn Ile Asn Asp Asp Asp Leu Val Tyr Ser Asn Ala              20 25 30 Thr Asp Leu Pro Val Gly Val Lys Lys Ser Pro Lys Met Ser Pro Thr          35 40 45 Ala Arg Val Gly Leu Leu Val Phe Gly Val Ile Ala Ala Val Gly Trp      50 55 60 Gly Ala Ile Ala Phe Ser Arg Gly Glu Thr Ile Asn Ser Val Trp Leu  65 70 75 80 Val Leu Ala Ala Val Gly Ser Tyr Ile Ile Ala Phe Ser Phe Tyr Ala                  85 90 95 Arg Leu Ile Glu Tyr Lys Val Val Lys Pro Lys Asp Gln Arg Ala Thr             100 105 110 Pro Ala Glu Tyr Val Asn Asp Gly Lys Asp Tyr Val Pro Thr Asp Arg         115 120 125 Arg Val Leu Phe Gly His His Phe Ala Ala Ile Ala Gly Ala Gly Pro     130 135 140 Leu Val Gly Pro Val Met Ala Ala Gln Met Gly Tyr Leu Pro Gly Thr 145 150 155 160 Leu Trp Ile Ile Leu Gly Val Ile Phe Ala Gly Ala Val Gln Asp Tyr                 165 170 175 Leu Val Leu Trp Val Ser Thr Arg Arg Arg Gly Arg Ser Leu Gly Gln             180 185 190 Met Val Arg Asp Glu Met Gly Thr Val Gly Gly Ala Ala Gly Ile Leu         195 200 205 Ala Thr Ile Ser Ile Met Ile Ile Ile Ile Ala Val Leu Ala Leu Ile     210 215 220 Val Val Asn Ala Leu Ala Asp Ser Pro Trp Gly Val Phe Ser Ile Thr 225 230 235 240 Met Thr Ile Pro Ile Ala Leu Phe Met Gly Val Tyr Leu Arg Tyr Leu                 245 250 255 Arg Pro Gly Arg Val Thr Glu Val Ser Ile Gly Val Ala Leu Leu             260 265 270 Leu Leu Ala Ile Val Ala Gly Gly Trp Val Ala Asp Thr Ser Trp Gly         275 280 285 Val Glu Trp Phe Thr Trp Ser Lys Thr Thr Leu Ala Leu Ala Leu Ile     290 295 300 Gly Tyr Gly Ile Met Ala Ala Ile Leu Pro Val Trp Leu Leu Leu Ala 305 310 315 320 Pro Arg Asp Tyr Leu Ser Thr Phe Met Lys Ile Gly Val Ile Gly Leu                 325 330 335 Leu Ala Val Gly Ile Leu Phe Ala Arg Pro Glu Val Gln Met Pro Ser             340 345 350 Val Thr Ser Phe Ala Leu Glu Gly Asn Gly Pro Val Phe Ser Gly Ser         355 360 365 Leu Phe Pro Phe Leu Phe Ile Thr Ile Ala Cys Gly Ala Leu Ser Gly     370 375 380 Phe His Ala Leu Ile Ser Ser Gly Thr Thr Pro Lys Leu Val Glu Lys 385 390 395 400 Glu Ser Gln Met Arg Met Leu Gly Tyr Gly Gly Met Leu Met Glu Ser                 405 410 415 Phe Val Ala Met Met Ala Leu Ile Thr Ala Val Ile Leu Asp Arg His             420 425 430 Leu Tyr Phe Ser Met Asn Ala Pro Leu Ala Leu Thr Gly Gly Asp Pro         435 440 445 Ala Thr Ala Ala Glu Trp Val Asn Ser Ile Gly Leu Thr Gly Ala Asp     450 455 460 Ile Thr Pro Glu Gln Leu Ser Glu Ala Ala Glu Ser Val Gly Glu Ser 465 470 475 480 Thr Val Ile Ser Arg Thr Gly Gly Ala Pro Thr Leu Ala Phe Gly Met                 485 490 495 Ser Glu Ile Leu Ser Gly Phe Ile Gly Gly Ala Gly Met Lys Ala Phe             500 505 510 Trp Tyr His Phe Ala Ile Met Phe Glu Ala Leu Phe Ile Leu Thr Thr         515 520 525 Val Asp Ala Gly Thr Arg Val Ala Arg Phe Met Met Thr Asp Thr Leu     530 535 540 Gly Asn Val Pro Gly Leu Arg Arg Phe Lys Asp Pro Ser Trp Thr Val 545 550 555 560 Gly Asn Trp Ile Ser Thr Val Phe Val Cys Ala Leu Trp Gly Ala Ile                 565 570 575 Leu Leu Met Gly Val Thr Asp Pro Leu Gly Gly Ile Asn Val Leu Phe             580 585 590 Pro Leu Phe Gly Ile Ala Asn Gln Leu Leu Ala Ala Ile Ala Leu Ala         595 600 605 Leu Val Leu Val Val Val Val Lys Lys Gly Leu Tyr Lys Trp Ala Trp     610 615 620 Ile Pro Ala Val Pro Leu Ala Trp Asp Leu Ile Val Thr Met Thr Ala 625 630 635 640 Ser Trp Gln Lys Ile Phe His Ser Asp Pro Ala Ile Gly Tyr Trp Ala                 645 650 655 Gln Asn Ala Asn Phe Arg Asp Ala Lys Ser Gln Gly Leu Thr Glu Phe             660 665 670 Gly Ala Ala Lys Ser Pro Glu Ala Ile Asp Ala Val Ile Arg Asn Thr         675 680 685 Met Ile Gln Gly Ile Leu Ser Ile Leu Phe Ala Val Leu Val Leu Val     690 695 700 Val Val Gly Ala Ala Ile Ala Val Cys Ile Lys Ser Ile Arg Ala Arg 705 710 715 720 Ala Ala Gly Thr Pro Leu Glu Thr Thr Glu Glu Pro Asp Thr Glu Ser                 725 730 735 Glu Phe Phe Ala Pro Thr Gly Phe Leu Ala Ser Ser Arg Asp Lys Glu             740 745 750 Val Gln Ala Met Trp Asp Glu Arg Tyr Pro Gly Gly Ala Pro Val Ser         755 760 765 Ser Gly Gly His     770 <210> 15 <211> 255 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101) .. (232) <223> RXN03119 <400> 15 tgggaggtgt cgcaccaagt acttttgcga agcgccatct gacggatttt caaaagatgt 60 atatgctcgg tgcggaaacc tacgaaagga ttttttaccc atg gct gta tac gaa 115                                             Met Ala Val Tyr Glu                                               1 5 ctc cca gaa ctc gac tac gca tac gac gct ctc gag cca cac atc gtc 163 Leu Pro Glu Leu Asp Tyr Ala Tyr Asp Ala Leu Glu Pro His Ile Val                  10 15 20 gct gaa atc atg gag ctt gac cag tcc aag gac cac gca acc tac gtt 211 Ala Glu Ile Met Glu Leu Asp Gln Ser Lys Asp His Ala Thr Tyr Val              25 30 35 gcg ggc gca aat gca gca ctc taggcactag agaaggcacg cga 255 Ala Gly Ala Asn Ala Ala Leu          40 <210> 16 <211> 44 <212> PRT <213> Corynebacterium glutamicum <400> 16 Met Ala Val Tyr Glu Leu Pro Glu Leu Asp Tyr Ala Tyr Asp Ala Leu   1 5 10 15 Glu Pro His Ile Val Ala Glu Ile Met Glu Leu Asp Gln Ser Lys Asp              20 25 30 His Ala Thr Tyr Val Ala Gly Ala Asn Ala Ala Leu          35 40 <210> 17 <211> 249 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (226) <223> RXN03120 <400> 17 ctgggcagtt cttgggtacg accacatatc cggtcgcctg gttatcgagc agctcaccga 60 ccaggagggc aacatctcct tcgacatcac cccagttctg atg ctc gat atg tgg 115                                             Met Leu Asp Met Trp                                               1 5 gag cac gct ttc tac ctg cag tac atg aac gtt aag gca gat tac gtc 163 Glu His Ala Phe Tyr Leu Gln Tyr Met Asn Val Lys Ala Asp Tyr Val                  10 15 20 aag gct gtt tgg aac gtc ttc aac tgg gac gac gca aga gca cgc ttc 211 Lys Ala Val Trp Asn Val Phe Asn Trp Asp Asp Ala Arg Ala Arg Phe              25 30 35 gca gca gct tcc aag taagcatttt tagtccgtgc aat 249 Ala Ala Ala Ser Lys          40 <210> 18 <211> 42 <212> PRT <213> Corynebacterium glutamicum <400> 18 Met Leu Asp Met Trp Glu His Ala Phe Tyr Leu Gln Tyr Met Asn Val   1 5 10 15 Lys Ala Asp Tyr Val Lys Ala Val Trp Asn Val Phe Asn Trp Asp Asp              20 25 30 Ala Arg Ala Arg Phe Ala Ala Ala Ser Lys          35 40 <210> 19 <211> 660 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101) .. (637) <223> RXN00575 <400> 19 gaagcgtccg caggcaagaa aacgtcccgg aaaacggcga taggggtcac ccgcgcatgt 60 ccggttgccg atctatttaa ataccaggac aattgcgtgc atg gtt gaa aga gac 115                                             Met val glu arg asp                                               1 5 ttc act atc cga cca atc cgc gag ggt gat ttc cct cag gtg agg gac 163 Phe Thr Ile Arg Pro Ile Arg Glu Gly Asp Phe Pro Gln Val Arg Asp                  10 15 20 atc tac gaa ttg ggc ctg gag acg gga cat gcg act tat gag act tct 211 Ile Tyr Glu Leu Gly Leu Glu Thr Gly His Ala Thr Tyr Glu Thr Ser              25 30 35 ggt ccc acg tgg gac cag ttc tcc caa tct aaa atc atg gat acc gtc 259 Gly Pro Thr Trp Asp Gln Phe Ser Gln Ser Lys Ile Met Asp Thr Val          40 45 50 atg gtg gcg gta gaa aac aac gac ccg gac ttc atc ctc gga tgg gtg 307 Met Val Ala Val Glu Asn Asn Asp Pro Asp Phe Ile Leu Gly Trp Val      55 60 65 tct gct gct cca att tca agc cga cag gtt ttc cat gga gtg gtg gaa 355 Ser Ala Ala Pro Ile Ser Ser Arg Gln Val Phe His Gly Val Val Glu  70 75 80 85 gat tcc atc tat atc cac ccc cag ggc caa ggc cga gga atc ggc ggc 403 Asp Ser Ile Tyr Ile His Pro Gln Gly Gln Gly Arg Gly Ile Gly Gly                  90 95 100 gct ttg ctc gac gcc ctt atc acc tac tgc gaa agc aac ggc atc tgg 451 Ala Leu Leu Asp Ala Leu Ile Thr Tyr Cys Glu Ser Asn Gly Ile Trp             105 110 115 tcg atc cac tcc tgg atc ttc ccg gaa aac ctc ggt tct gcg aaa ctg 499 Ser Ile His Ser Trp Ile Phe Pro Glu Asn Leu Gly Ser Ala Lys Leu         120 125 130 cat gaa tcg aag ggc ttc gtg aag gtg ggc acc atg cac caa atg gca 547 His Glu Ser Lys Gly Phe Val Lys Val Gly Thr Met His Gln Met Ala     135 140 145 agg atg ccc tac ggc gag atg gaa gga caa tgg cgc gat tgt gat ctg 595 Arg Met Pro Tyr Gly Glu Met Glu Gly Gln Trp Arg Asp Cys Asp Leu 150 155 160 165 tgg gag tgc ctc tta tcc gtt cca gag caa gct caa agt tcc 637 Trp Glu Cys Leu Leu Ser Val Pro Glu Gln Ala Gln Ser Ser                 170 175 taaagcaatt taaatctgac ttt 660 <210> 20 <211> 179 <212> PRT <213> Corynebacterium glutamicum <400> 20 Met Val Glu Arg Asp Phe Thr Ile Arg Pro Ile Arg Glu Gly Asp Phe   1 5 10 15 Pro Gln Val Arg Asp Ile Tyr Glu Leu Gly Leu Glu Thr Gly His Ala              20 25 30 Thr Tyr Glu Thr Ser Gly Pro Thr Trp Asp Gln Phe Ser Gln Ser Lys          35 40 45 Ile Met Asp Thr Val Met Val Ala Val Glu Asn Asn Asp Pro Asp Phe      50 55 60 Ile Leu Gly Trp Val Ser Ala Ala Pro Ile Ser Ser Arg Gln Val Phe  65 70 75 80 His Gly Val Val Glu Asp Ser Ile Tyr Ile His Pro Gln Gly Gln Gly                  85 90 95 Arg Gly Ile Gly Gly Ala Leu Leu Asp Ala Leu Ile Thr Tyr Cys Glu             100 105 110 Ser Asn Gly Ile Trp Ser Ile His Ser Trp Ile Phe Pro Glu Asn Leu         115 120 125 Gly Ser Ala Lys Leu His Glu Ser Lys Gly Phe Val Lys Val Gly Thr     130 135 140 Met His Gln Met Ala Arg Met Pro Tyr Gly Glu Met Glu Gly Gln Trp 145 150 155 160 Arg Asp Cys Asp Leu Trp Glu Cys Leu Leu Ser Val Pro Glu Gln Ala                 165 170 175 Gln ser ser              <210> 21 <211> 606 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (101) .. (583) <223> FRXA00575 <400> 21 gcatgtccgg ttgccgatct atttaaatac caggacaatt gcgtgcatgg ttgaaagaga 60 cttcactatc cgaccaatcc gcgagggtga tttccctcag gtg agg gac atc tac 115                                             Val Arg Asp Ile Tyr                                               1 5 gaa ttg ggc ctg gag acg gga cat gcg act tat gag act tct ggt ccc 163 Glu Leu Gly Leu Glu Thr Gly His Ala Thr Tyr Glu Thr Ser Gly Pro                  10 15 20 acg tgg gac cag ttc tcc caa tct aaa atc atg gat acc gtc atg gtg 211 Thr Trp Asp Gln Phe Ser Gln Ser Lys Ile Met Asp Thr Val Met Val              25 30 35 gcg gta gaa aac aac gac ccg gac ttc atc ctc gga tgg gtg tct gct 259 Ala Val Glu Asn Asn Asp Pro Asp Phe Ile Leu Gly Trp Val Ser Ala          40 45 50 gct cca att tca agc cga cag gtt ttc cat gga gtg gtg gaa gat tcc 307 Ala Pro Ile Ser Ser Arg Gln Val Phe His Gly Val Val Glu Asp Ser      55 60 65 atc tat atc cac ccc cag ggc caa ggc cga gga atc ggc ggc gct ttg 355 Ile Tyr Ile His Pro Gln Gly Gln Gly Arg Gly Ile Gly Gly Ala Leu  70 75 80 85 ctc gac gcc ctt atc acc tac tgc gaa agc aac ggc atc tgg tcg atc 403 Leu Asp Ala Leu Ile Thr Tyr Cys Glu Ser Asn Gly Ile Trp Ser Ile                  90 95 100 cac tcc tgg atc ttc ccg gaa aac ctc ggt tct gcg aaa ctg cat gaa 451 His Ser Trp Ile Phe Pro Glu Asn Leu Gly Ser Ala Lys Leu His Glu             105 110 115 tcg aag ggc ttc gtg aag gtg ggc acc atg cac caa atg gca agg atg 499 Ser Lys Gly Phe Val Lys Val Gly Thr Met His Gln Met Ala Arg Met         120 125 130 ccc tac ggc gag atg gaa gga caa tgg cgc gat tgt gat ctg tgg gag 547 Pro Tyr Gly Glu Met Glu Gly Gln Trp Arg Asp Cys Asp Leu Trp Glu     135 140 145 tgc ctc tta tcc gtt cca gag caa gct caa agt tcc taaagcaatt 593 Cys Leu Leu Ser Val Pro Glu Gln Ala Gln Ser Ser 150 155 160 taaatctgac ttt 606 <210> 22 <211> 161 <212> PRT <213> Corynebacterium glutamicum <400> 22 Val Arg Asp Ile Tyr Glu Leu Gly Leu Glu Thr Gly His Ala Thr Tyr   1 5 10 15 Glu Thr Ser Gly Pro Thr Trp Asp Gln Phe Ser Gln Ser Lys Ile Met              20 25 30 Asp Thr Val Met Val Ala Val Glu Asn Asn Asp Pro Asp Phe Ile Leu          35 40 45 Gly Trp Val Ser Ala Ala Pro Ile Ser Ser Arg Gln Val Phe His Gly      50 55 60 Val Val Glu Asp Ser Ile Tyr Ile His Pro Gln Gly Gln Gly Arg Gly  65 70 75 80 Ile Gly Gly Ala Leu Leu Asp Ala Leu Ile Thr Tyr Cys Glu Ser Asn                  85 90 95 Gly Ile Trp Ser Ile His Ser Trp Ile Phe Pro Glu Asn Leu Gly Ser             100 105 110 Ala Lys Leu His Glu Ser Lys Gly Phe Val Lys Val Gly Thr Met His         115 120 125 Gln Met Ala Arg Met Pro Tyr Gly Glu Met Glu Gly Gln Trp Arg Asp     130 135 140 Cys Asp Leu Trp Glu Cys Leu Leu Ser Val Pro Glu Gln Ala Gln Ser 145 150 155 160 Ser      <210> 23 <211> 1575 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1552) <223> RXN01345 <400> 23 cataacctca ttgaacatgc aaaactaatg cttttggggg gtatgcataa attcgtttcg 60 ttccactgca cagcccgaaa atgctgctag ggtcaagttc atg cgt ttt gga ctt 115                                             Met Arg Phe Gly Leu                                               1 5 gac ttg gga act acc cgc aca atc gcg gcc gcc gtg gac cgc gga aac 163 Asp Leu Gly Thr Thr Arg Thr Ile Ala Ala Ala Val Asp Arg Gly Asn                  10 15 20 tat ccc atc gtc act gtg gaa gat tct tta ggc gac acc cac gat ttc 211 Tyr Pro Ile Val Thr Val Glu Asp Ser Leu Gly Asp Thr His Asp Phe              25 30 35 att cca tct gtg gtg gcc ctc aag gca gat agg att gtc gcg ggt tgg 259 Ile Pro Ser Val Val Ala Leu Lys Ala Asp Arg Ile Val Ala Gly Trp          40 45 50 gat gct att gag gtt ggg cag gac cac cct tcc ttc gta cgt tct ttc 307 Asp Ala Ile Glu Val Gly Gln Asp His Pro Ser Phe Val Arg Ser Phe      55 60 65 aaa cgc cta ctc tct gaa ccc aat gtc acg gaa gcc acc ccg gtc tac 355 Lys Arg Leu Leu Ser Glu Pro Asn Val Thr Glu Ala Thr Pro Val Tyr  70 75 80 85 ttg ggc gat cat gta cac cct ttg ggc gcc gtc ctg gag gct ttt gcg 403 Leu Gly Asp His Val His Pro Leu Gly Ala Val Leu Glu Ala Phe Ala                  90 95 100 gaa aac gtg gtc act gcg ctg cgt gca ttt cag acg caa ttg gga gat 451 Glu Asn Val Val Thr Ala Leu Arg Ala Phe Gln Thr Gln Leu Gly Asp             105 110 115 acc tcc ccg atc gaa gta gtc att ggt gtg ccc gcc aac tcc cac agc 499 Thr Ser Pro Ile Glu Val Val Ile Gly Val Pro Ala Asn Ser His Ser         120 125 130 gcc cag cga ctg ctc acc atg tcc gcc ttc agc gcc aca ggc atc acc 547 Ala Gln Arg Leu Leu Thr Met Ser Ala Phe Ser Ala Thr Gly Ile Thr     135 140 145 gtt gtc ggt ttg gtc aat gag ccc agc gcc gca gct ttc gag tac acc 595 Val Val Gly Leu Val Asn Glu Pro Ser Ala Ala Ala Phe Glu Tyr Thr 150 155 160 165 cac cgc cac gcc cgc acc tta aac tcc aag cgc caa gcc atc gtg gtt 643 His Arg His Ala Arg Thr Leu Asn Ser Lys Arg Gln Ala Ile Val Val                 170 175 180 tat gat ttg gga ggc gga aca ttc gac tcc tcg ctc atc cgc atc gac 691 Tyr Asp Leu Gly Gly Gly Thr Phe Asp Ser Ser Leu Ile Arg Ile Asp             185 190 195 ggc acc cac cac gag gtt gtg tcc tcc att ggc att tca cgc ctt ggt 739 Gly Thr His His Glu Val Val Ser Ser Ile Gly Ile Ser Arg Leu Gly         200 205 210 ggc gat gat ttc gat gaa atc ctc ctc caa tgc gcg ctc aag gcc gca 787 Gly Asp Asp Phe Asp Glu Ile Leu Leu Gln Cys Ala Leu Lys Ala Ala     215 220 225 ggc aga cag cac gat gcg ttt ggc aag cgt gct aaa aac acg ctt ctc 835 Gly Arg Gln His Asp Ala Phe Gly Lys Arg Ala Lys Asn Thr Leu Leu 230 235 240 245 gac gaa tcc cgc aac gcg aag gaa gct ctt gtt ccg caa tcc cgt cgc 883 Asp Glu Ser Arg Asn Ala Lys Glu Ala Leu Val Pro Gln Ser Arg Arg                 250 255 260 ttg gtt cta gaa att ggc gac gac gac atc acc gtt cca gtg aac aag 931 Leu Val Leu Glu Ile Gly Asp Asp Asp Ile Thr Val Pro Val Asn Lys             265 270 275 ttc tac gag gct gcc act ccc ctg gtg gaa aaa tcc ttg tcc atc atg 979 Phe Tyr Glu Ala Ala Thr Pro Leu Val Glu Lys Ser Leu Ser Ile Met         280 285 290 gaa ccc ctc atc ggc gtc gat gat ctt aaa gat tcc gac atc gca ggc 1027 Glu Pro Leu Ile Gly Val Asp Asp Leu Lys Asp Ser Asp Ile Ala Gly     295 300 305 atc tac ctt gtt ggt gga gga tcc tcg ctc cca ctc gtt tcc agg ttg 1075 Ile Tyr Leu Val Gly Gly Gly Ser Ser Leu Pro Leu Val Ser Arg Leu 310 315 320 325 ctc cgc gag cgt ttc ggc cgc cgt gtc cac cgc tcc cca ttc ccc tca 1123 Leu Arg Glu Arg Phe Gly Arg Arg Val His Arg Ser Pro Phe Pro Ser                 330 335 340 ggt tcc act gcg gtg ggt ctg gcc atc gcg gct gac cct tcc tct ggt 1171 Gly Ser Thr Ala Val Gly Leu Ala Ile Ala Ala Asp Pro Ser Ser Gly             345 350 355 ttc cac cta agg gac cgc gtt gcg cga ggc atc ggt gtg ttc cgt gag 1219 Phe His Leu Arg Asp Arg Val Ala Arg Gly Ile Gly Val Phe Arg Glu         360 365 370 cac gat tct ggt cgt gcc gtg agc ttt gac ccg ctg atc gcc ccg gac 1267 His Asp Ser Gly Arg Ala Val Ser Phe Asp Pro Leu Ile Ala Pro Asp     375 380 385 acc gat tct gcg acc gtg gcg aaa cga tgc tac aag gcg gtg cac aac 1315 Thr Asp Ser Ala Thr Val Ala Lys Arg Cys Tyr Lys Ala Val His Asn 390 395 400 405 att ggt tgg ttc agg ttc gtg gaa tac tcc acc gtg tcc gag gat ggc 1363 Ile Gly Trp Phe Arg Phe Val Glu Tyr Ser Thr Val Ser Glu Asp Gly                 410 415 420 agc ccc gga gat att tcc ctg ctc agt gaa atc aag att cct ttt gat 1411 Ser Pro Gly Asp Ile Ser Leu Leu Ser Glu Ile Lys Ile Pro Phe Asp             425 430 435 agc tcc atc acc gat gtg gat gct acc gag att tca cgt ttc gat ggc 1459 Ser Ser Ile Thr Asp Val Asp Ala Thr Glu Ile Ser Arg Phe Asp Gly         440 445 450 cca gaa gta gaa gaa acc atc aca gtc aat gac aac ggc gtg gct tcc 1507 Pro Glu Val Glu Glu Thr Ile Thr Val Asn Asp Asn Gly Val Ala Ser     455 460 465 att tcc atc aag ata ctc ggc ggc gtt acc gtc gag cac aca att 1552 Ile Ser Ile Lys Ile Leu Gly Gly Val Thr Val Glu His Thr Ile 470 475 480 tagttaccat tttggtgctg gtg 1575 <210> 24 <211> 484 <212> PRT <213> Corynebacterium glutamicum <400> 24 Met Arg Phe Gly Leu Asp Leu Gly Thr Thr Arg Thr Ile Ala Ala Ala   1 5 10 15 Val Asp Arg Gly Asn Tyr Pro Ile Val Thr Val Glu Asp Ser Leu Gly              20 25 30 Asp Thr His Asp Phe Ile Pro Ser Val Val Ala Leu Lys Ala Asp Arg          35 40 45 Ile Val Ala Gly Trp Asp Ala Ile Glu Val Gly Gln Asp His Pro Ser      50 55 60 Phe Val Arg Ser Phe Lys Arg Leu Leu Ser Glu Pro Asn Val Thr Glu  65 70 75 80 Ala Thr Pro Val Tyr Leu Gly Asp His Val His Pro Leu Gly Ala Val                  85 90 95 Leu Glu Ala Phe Ala Glu Asn Val Val Thr Ala Leu Arg Ala Phe Gln             100 105 110 Thr Gln Leu Gly Asp Thr Ser Pro Ile Glu Val Val Ile Gly Val Pro         115 120 125 Ala Asn Ser His Ser Ala Gln Arg Leu Leu Thr Met Ser Ala Phe Ser     130 135 140 Ala Thr Gly Ile Thr Val Val Gly Leu Val Asn Glu Pro Ser Ala Ala 145 150 155 160 Ala Phe Glu Tyr Thr His Arg His Ala Arg Thr Leu Asn Ser Lys Arg                 165 170 175 Gln Ala Ile Val Val Tyr Asp Leu Gly Gly Gly Thr Phe Asp Ser Ser             180 185 190 Leu Ile Arg Ile Asp Gly Thr His His Glu Val Val Ser Ser Ile Gly         195 200 205 Ile Ser Arg Leu Gly Gly Asp Asp Phe Asp Glu Ile Leu Leu Gln Cys     210 215 220 Ala Leu Lys Ala Ala Gly Arg Gln His Asp Ala Phe Gly Lys Arg Ala 225 230 235 240 Lys Asn Thr Leu Leu Asp Glu Ser Arg Asn Ala Lys Glu Ala Leu Val                 245 250 255 Pro Gln Ser Arg Arg Leu Val Leu Glu Ile Gly Asp Asp Asp Ile Thr             260 265 270 Val Pro Val Asn Lys Phe Tyr Glu Ala Ala Thr Pro Leu Val Glu Lys         275 280 285 Ser Leu Ser Ile Met Glu Pro Leu Ile Gly Val Asp Asp Leu Lys Asp     290 295 300 Ser Asp Ile Ala Gly Ile Tyr Leu Val Gly Gly Gly Ser Ser Leu Pro 305 310 315 320 Leu Val Ser Arg Leu Leu Arg Glu Arg Phe Gly Arg Arg Val His Arg                 325 330 335 Ser Pro Phe Pro Ser Gly Ser Thr Ala Val Gly Leu Ala Ile Ala Ala             340 345 350 Asp Pro Ser Ser Gly Phe His Leu Arg Asp Arg Val Ala Arg Gly Ile         355 360 365 Gly Val Phe Arg Glu His Asp Ser Gly Arg Ala Val Ser Phe Asp Pro     370 375 380 Leu Ile Ala Pro Asp Thr Asp Ser Ala Thr Val Ala Lys Arg Cys Tyr 385 390 395 400 Lys Ala Val His Asn Ile Gly Trp Phe Arg Phe Val Glu Tyr Ser Thr                 405 410 415 Val Ser Glu Asp Gly Ser Pro Gly Asp Ile Ser Leu Leu Ser Glu Ile             420 425 430 Lys Ile Pro Phe Asp Ser Ser Ile Thr Asp Val Asp Ala Thr Glu Ile         435 440 445 Ser Arg Phe Asp Gly Pro Glu Val Glu Glu Thr Ile Thr Val Asn Asp     450 455 460 Asn Gly Val Ala Ser Ile Ser Ile Lys Ile Leu Gly Gly Val Thr Val 465 470 475 480 Glu His Thr Ile                  <210> 25 <211> 1267 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (1267) <223> FRXA01345 <400> 25 cataacctca ttgaacatgc aaaactaatg cttttggggg gtatgcataa attcgtttcg 60 ttccactgca cagcccgaaa atgctgctag ggtcaagttc atg cgt ttt gga ctt 115                                             Met Arg Phe Gly Leu                                               1 5 gac ttg gga act acc cgc aca atc gcg gcc gcc gtg gac cgc gga aac 163 Asp Leu Gly Thr Thr Arg Thr Ile Ala Ala Ala Val Asp Arg Gly Asn                  10 15 20 tat ccc atc gtc act gtg gaa gat tct tta ggc gac acc cac gat ttc 211 Tyr Pro Ile Val Thr Val Glu Asp Ser Leu Gly Asp Thr His Asp Phe              25 30 35 att cca tct gtg gtg gcc ctc aag gca gat agg att gtc gcg ggt tgg 259 Ile Pro Ser Val Val Ala Leu Lys Ala Asp Arg Ile Val Ala Gly Trp          40 45 50 gat gct att gag gtt ggg cag gac cac cct tcc ttc gta cgt tct ttc 307 Asp Ala Ile Glu Val Gly Gln Asp His Pro Ser Phe Val Arg Ser Phe      55 60 65 aaa cgc cta ctc tct gaa ccc aat gtc acg gaa gcc acc ccg gtc tac 355 Lys Arg Leu Leu Ser Glu Pro Asn Val Thr Glu Ala Thr Pro Val Tyr  70 75 80 85 ttg ggc gat cat gta cac cct ttg ggc gcc gtc ctg gag gct ttt gcg 403 Leu Gly Asp His Val His Pro Leu Gly Ala Val Leu Glu Ala Phe Ala                  90 95 100 gaa aac gtg gtc act gcg ctg cgt gca ttt cag acg caa ttg gga gat 451 Glu Asn Val Val Thr Ala Leu Arg Ala Phe Gln Thr Gln Leu Gly Asp             105 110 115 acc tcc ccg atc gaa gta gtc att ggt gtg ccc gcc aac tcc cac agc 499 Thr Ser Pro Ile Glu Val Val Ile Gly Val Pro Ala Asn Ser His Ser         120 125 130 gcc cag cga ctg ctc acc atg tcc gcc ttc agc gcc aca ggc atc acc 547 Ala Gln Arg Leu Leu Thr Met Ser Ala Phe Ser Ala Thr Gly Ile Thr     135 140 145 gtt gtc ggt ttg gtc aat gag ccc agc gcc gca gct ttc gag tac acc 595 Val Val Gly Leu Val Asn Glu Pro Ser Ala Ala Ala Phe Glu Tyr Thr 150 155 160 165 cac cgc cac gcc cgc acc tta aac tcc aag cgc caa gcc atc gtg gtt 643 His Arg His Ala Arg Thr Leu Asn Ser Lys Arg Gln Ala Ile Val Val                 170 175 180 tat gat ttg gga ggc gga aca ttc gac tcc tcg ctc atc cgc atc gac 691 Tyr Asp Leu Gly Gly Gly Thr Phe Asp Ser Ser Leu Ile Arg Ile Asp             185 190 195 ggc acc cac cac gag gtt gtg tcc tcc att ggc att tca cgc ctt ggt 739 Gly Thr His His Glu Val Val Ser Ser Ile Gly Ile Ser Arg Leu Gly         200 205 210 ggc gat gat ttc gat gaa atc ctc ctc caa tgc gcg ctc aag gcc gca 787 Gly Asp Asp Phe Asp Glu Ile Leu Leu Gln Cys Ala Leu Lys Ala Ala     215 220 225 ggc aga cag cac gat gcg ttt ggc aag cgt gct aaa aac acg ctt ctc 835 Gly Arg Gln His Asp Ala Phe Gly Lys Arg Ala Lys Asn Thr Leu Leu 230 235 240 245 gac gaa tcc cgc aac gcg aag gaa gct ctt gtt ccg caa tcc cgt cgc 883 Asp Glu Ser Arg Asn Ala Lys Glu Ala Leu Val Pro Gln Ser Arg Arg                 250 255 260 ttg gtt cta gaa att ggc gac gac gac atc acc gtt cca gtg aac aag 931 Leu Val Leu Glu Ile Gly Asp Asp Asp Ile Thr Val Pro Val Asn Lys             265 270 275 ttc tac gag gct gcc act ccc ctg gtg gaa aaa tcc ttg tcc atc atg 979 Phe Tyr Glu Ala Ala Thr Pro Leu Val Glu Lys Ser Leu Ser Ile Met         280 285 290 gaa ccc ctc atc ggc gtc gat gat ctt aaa gat tcc gac atc gca ggc 1027 Glu Pro Leu Ile Gly Val Asp Asp Leu Lys Asp Ser Asp Ile Ala Gly     295 300 305 atc tac ctt gtt ggt gga gga tcc tcg ctc cca ctc gtt tcc agg ttg 1075 Ile Tyr Leu Val Gly Gly Gly Ser Ser Leu Pro Leu Val Ser Arg Leu 310 315 320 325 ctc cgc gag cgt ttc ggc cgc cgt gtc cac cgc tcc cca ttc ccc tca 1123 Leu Arg Glu Arg Phe Gly Arg Arg Val His Arg Ser Pro Phe Pro Ser                 330 335 340 ggt tcc act gcg gtg ggt ctg gcc atc gcg gct gac cct tcc tct ggt 1171 Gly Ser Thr Ala Val Gly Leu Ala Ile Ala Ala Asp Pro Ser Ser Gly             345 350 355 ttc cac cta agg gac cgc gtt gcg cga ggc atc ggt gtg ttc cgt gag 1219 Phe His Leu Arg Asp Arg Val Ala Arg Gly Ile Gly Val Phe Arg Glu         360 365 370 cac gat tct ggt cgt gcc gtg agc ttt gac ccg ctg atc gcc ccg gac 1267 His Asp Ser Gly Arg Ala Val Ser Phe Asp Pro Leu Ile Ala Pro Asp     375 380 385 <210> 26 <211> 389 <212> PRT <213> Corynebacterium glutamicum <400> 26 Met Arg Phe Gly Leu Asp Leu Gly Thr Thr Arg Thr Ile Ala Ala Ala   1 5 10 15 Val Asp Arg Gly Asn Tyr Pro Ile Val Thr Val Glu Asp Ser Leu Gly              20 25 30 Asp Thr His Asp Phe Ile Pro Ser Val Val Ala Leu Lys Ala Asp Arg          35 40 45 Ile Val Ala Gly Trp Asp Ala Ile Glu Val Gly Gln Asp His Pro Ser      50 55 60 Phe Val Arg Ser Phe Lys Arg Leu Leu Ser Glu Pro Asn Val Thr Glu  65 70 75 80 Ala Thr Pro Val Tyr Leu Gly Asp His Val His Pro Leu Gly Ala Val                  85 90 95 Leu Glu Ala Phe Ala Glu Asn Val Val Thr Ala Leu Arg Ala Phe Gln             100 105 110 Thr Gln Leu Gly Asp Thr Ser Pro Ile Glu Val Val Ile Gly Val Pro         115 120 125 Ala Asn Ser His Ser Ala Gln Arg Leu Leu Thr Met Ser Ala Phe Ser     130 135 140 Ala Thr Gly Ile Thr Val Val Gly Leu Val Asn Glu Pro Ser Ala Ala 145 150 155 160 Ala Phe Glu Tyr Thr His Arg His Ala Arg Thr Leu Asn Ser Lys Arg                 165 170 175 Gln Ala Ile Val Val Tyr Asp Leu Gly Gly Gly Thr Phe Asp Ser Ser             180 185 190 Leu Ile Arg Ile Asp Gly Thr His His Glu Val Val Ser Ser Ile Gly         195 200 205 Ile Ser Arg Leu Gly Gly Asp Asp Phe Asp Glu Ile Leu Leu Gln Cys     210 215 220 Ala Leu Lys Ala Ala Gly Arg Gln His Asp Ala Phe Gly Lys Arg Ala 225 230 235 240 Lys Asn Thr Leu Leu Asp Glu Ser Arg Asn Ala Lys Glu Ala Leu Val                 245 250 255 Pro Gln Ser Arg Arg Leu Val Leu Glu Ile Gly Asp Asp Asp Ile Thr             260 265 270 Val Pro Val Asn Lys Phe Tyr Glu Ala Ala Thr Pro Leu Val Glu Lys         275 280 285 Ser Leu Ser Ile Met Glu Pro Leu Ile Gly Val Asp Asp Leu Lys Asp     290 295 300 Ser Asp Ile Ala Gly Ile Tyr Leu Val Gly Gly Gly Ser Ser Leu Pro 305 310 315 320 Leu Val Ser Arg Leu Leu Arg Glu Arg Phe Gly Arg Arg Val His Arg                 325 330 335 Ser Pro Phe Pro Ser Gly Ser Thr Ala Val Gly Leu Ala Ile Ala Ala             340 345 350 Asp Pro Ser Ser Gly Phe His Leu Arg Asp Arg Val Ala Arg Gly Ile         355 360 365 Gly Val Phe Arg Glu His Asp Ser Gly Arg Ala Val Ser Phe Asp Pro     370 375 380 Leu Ile Ala Pro Asp 385 <210> 27 <211> 1308 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1285) <223> RXA02541 <400> 27 atccgccggt gtccggacaa caaaacttgc aacacaagat aacttaagaa attgcataca 60 attcaccgca tataagactc atggaaggag gggatgccca gtg aac aac agc gaa 115                                             Val Asn Asn Ser Glu                                               1 5 tgg gca aat aag aac tat tac gca gac ctg ggg gtc tcc tcg tcc gct 163 Trp Ala Asn Lys Asn Tyr Tyr Ala Asp Leu Gly Val Ser Ser Ser Ala                  10 15 20 tca gaa gat gag atc aaa aag gct tac cgc aag ctc gcc agg gaa aat 211 Ser Glu Asp Glu Ile Lys Lys Ala Tyr Arg Lys Leu Ala Arg Glu Asn              25 30 35 cac cca gat aaa aat cca ggt gac aag gcc gct gaa gat cga ttc aaa 259 His Pro Asp Lys Asn Pro Gly Asp Lys Ala Ala Glu Asp Arg Phe Lys          40 45 50 aaa gcg gcc gag gca tat gac gta ctt ggt gat gac aag aaa cga aaa 307 Lys Ala Ala Glu Ala Tyr Asp Val Leu Gly Asp Asp Lys Lys Arg Lys      55 60 65 gaa tac gac gag ctc aaa gca ctt cta gct tct ggt gga atc cgc gga 355 Glu Tyr Asp Glu Leu Lys Ala Leu Leu Ala Ser Gly Gly Ile Arg Gly  70 75 80 85 gga ttc gga agc gga ggt gcg gga ttc ccc ggc ggg ttt cgc acg tcg 403 Gly Phe Gly Ser Gly Gly Ala Gly Phe Pro Gly Gly Phe Arg Thr Ser                  90 95 100 acg gga gga ttc gac acc tca gac ctc ttc gga gga gga caa ggt gga 451 Thr Gly Gly Phe Asp Thr Ser Asp Leu Phe Gly Gly Gly Gln Gly Gly             105 110 115 ggg ttt tct acg gac ggc ggt ttg ggc gat atc ttc ggt ggc ctt ttc 499 Gly Phe Ser Thr Asp Gly Gly Leu Gly Asp Ile Phe Gly Gly Leu Phe         120 125 130 aac cgc ggc gct ggt tct cac cag tca gct agg ccg acg cgg ggg gcg 547 Asn Arg Gly Ala Gly Ser His Gln Ser Ala Arg Pro Thr Arg Gly Ala     135 140 145 gat gta caa acc gaa ata act ctc tcg ttt gtt gag gca gcg aaa ggc 595 Asp Val Gln Thr Glu Ile Thr Leu Ser Phe Val Glu Ala Ala Lys Gly 150 155 160 165 acg acc atc cca gtg gaa ctc acc ggc gat gcg ccc tgc aac acc tgc 643 Thr Thr Ile Pro Val Glu Leu Thr Gly Asp Ala Pro Cys Asn Thr Cys                 170 175 180 cac gga tcg ggc tcc aaa tca ggc cac ccc gca aaa tgt gga acc tgt 691 His Gly Ser Gly Ser Lys Ser Gly His Pro Ala Lys Cys Gly Thr Cys             185 190 195 gat gga acc gga ttc acc tct gag aac aag ggt gct ttc gga ttc tcc 739 Asp Gly Thr Gly Phe Thr Ser Glu Asn Lys Gly Ala Phe Gly Phe Ser         200 205 210 gct cca tgt gca acc tgt ggt ggc act ggt gaa ata atc act gat cct 787 Ala Pro Cys Ala Thr Cys Gly Gly Thr Gly Glu Ile Ile Thr Asp Pro     215 220 225 tgc gat aac tgc cac ggc cga ggc acc gtc cgg aag tct cgt tcc atc 835 Cys Asp Asn Cys His Gly Arg Gly Thr Val Arg Lys Ser Arg Ser Ile 230 235 240 245 acc gtg cgt atc cca act ggt gtg gaa gat gga cag aaa gtt cgt ctt 883 Thr Val Arg Ile Pro Thr Gly Val Glu Asp Gly Gln Lys Val Arg Leu                 250 255 260 gca ggc caa ggc gaa gca gga cca aat ggc aaa cca gcg ggc gat ctc 931 Ala Gly Gln Gly Glu Ala Gly Pro Asn Gly Lys Pro Ala Gly Asp Leu             265 270 275 ttt gtg aaa gtc cac gtg aaa aag gac gat gtg ttc aca cgc gac ggc 979 Phe Val Lys Val His Val Lys Lys Asp Asp Val Phe Thr Arg Asp Gly         280 285 290 agc aac att ttg atc acc att ccc gtg agc ttc agc gag ctg gct ttg 1027 Ser Asn Ile Leu Ile Thr Ile Pro Val Ser Phe Ser Glu Leu Ala Leu     295 300 305 ggt ggc gct att tct gtg cca acg ctc aac aag cct gta aaa ctc aag 1075 Gly Gly Ala Ile Ser Val Pro Thr Leu Asn Lys Pro Val Lys Leu Lys 310 315 320 325 cta cct gcg gga acg cca gat ggt cgt act ttg cgt gta cgc ggt cgc 1123 Leu Pro Ala Gly Thr Pro Asp Gly Arg Thr Leu Arg Val Arg Gly Arg                 330 335 340 ggt atc gaa gca cgt gat tcc act ggt gat ctg ctg gtt aca gtc cag 1171 Gly Ile Glu Ala Arg Asp Ser Thr Gly Asp Leu Leu Val Thr Val Gln             345 350 355 gtt tct gtc ccg aag aat ctg gat gac aac gct gcg gaa gct ctc cgc 1219 Val Ser Val Pro Lys Asn Leu Asp Asp Asn Ala Ala Glu Ala Leu Arg         360 365 370 gca tat gct gaa gca gaa act aat tca ggt ttt gat ccc cgc gct aac 1267 Ala Tyr Ala Glu Ala Glu Thr Asn Ser Gly Phe Asp Pro Arg Ala Asn     375 380 385 tgg gcg ggc cag aac cgc tagacgttct ctttgagaaa gga 1308 Trp Ala Gly Gln Asn Arg 390 395 <210> 28 <211> 395 <212> PRT <213> Corynebacterium glutamicum <400> 28 Val Asn Asn Ser Glu Trp Ala Asn Lys Asn Tyr Tyr Ala Asp Leu Gly   1 5 10 15 Val Ser Ser Ser Ala Ser Glu Asp Glu Ile Lys Lys Ala Tyr Arg Lys              20 25 30 Leu Ala Arg Glu Asn His Pro Asp Lys Asn Pro Gly Asp Lys Ala Ala          35 40 45 Glu Asp Arg Phe Lys Lys Ala Ala Glu Ala Tyr Asp Val Leu Gly Asp      50 55 60 Asp Lys Lys Arg Lys Glu Tyr Asp Glu Leu Lys Ala Leu Leu Ala Ser  65 70 75 80 Gly Gly Ile Arg Gly Gly Phe Gly Ser Gly Gly Ala Gly Phe Pro Gly                  85 90 95 Gly Phe Arg Thr Ser Thr Gly Gly Phe Asp Thr Ser Asp Leu Phe Gly             100 105 110 Gly Gly Gln Gly Gly Gly Phe Ser Thr Asp Gly Gly Leu Gly Asp Ile         115 120 125 Phe Gly Gly Leu Phe Asn Arg Gly Ala Gly Ser His Gln Ser Ala Arg     130 135 140 Pro Thr Arg Gly Ala Asp Val Gln Thr Glu Ile Thr Leu Ser Phe Val 145 150 155 160 Glu Ala Ala Lys Gly Thr Thr Ile Pro Val Glu Leu Thr Gly Asp Ala                 165 170 175 Pro Cys Asn Thr Cys His Gly Ser Gly Ser Lys Ser Gly His Pro Ala             180 185 190 Lys Cys Gly Thr Cys Asp Gly Thr Gly Phe Thr Ser Glu Asn Lys Gly         195 200 205 Ala Phe Gly Phe Ser Ala Pro Cys Ala Thr Cys Gly Gly Thr Gly Glu     210 215 220 Ile Ile Thr Asp Pro Cys Asp Asn Cys His Gly Arg Gly Thr Val Arg 225 230 235 240 Lys Ser Arg Ser Ile Thr Val Arg Ile Pro Thr Gly Val Glu Asp Gly                 245 250 255 Gln Lys Val Arg Leu Ala Gly Gln Gly Glu Ala Gly Pro Asn Gly Lys             260 265 270 Pro Ala Gly Asp Leu Phe Val Lys Val His Val Lys Lys Asp Asp Val         275 280 285 Phe Thr Arg Asp Gly Ser Asn Ile Leu Ile Thr Ile Pro Val Ser Phe     290 295 300 Ser Glu Leu Ala Leu Gly Gly Ala Ile Ser Val Pro Thr Leu Asn Lys 305 310 315 320 Pro Val Lys Leu Lys Leu Pro Ala Gly Thr Pro Asp Gly Arg Thr Leu                 325 330 335 Arg Val Arg Gly Arg Gly Ile Glu Ala Arg Asp Ser Thr Gly Asp Leu             340 345 350 Leu Val Thr Val Gln Val Ser Val Pro Lys Asn Leu Asp Asp Asn Ala         355 360 365 Ala Glu Ala Leu Arg Ala Tyr Ala Glu Ala Glu Thr Asn Ser Gly Phe     370 375 380 Asp Pro Arg Ala Asn Trp Ala Gly Gln Asn Arg 385 390 395 <210> 29 <211> 777 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101) .. (754) <223> RXA02542 <400> 29 ccaggctgac gcaggtgcag aaggcgctgc agatgacaat gttgttgacg ctgaagttgt 60 cgaagacgac gcagctgaca atggtgagga caagaagtaa atg act acc cct aac 115                                             Met thr thr pro asn                                               1 5 gga atg ccc gac aat cct ggg gat cct gaa aat acc gat cca gag gca 163 Gly Met Pro Asp Asn Pro Gly Asp Pro Glu Asn Thr Asp Pro Glu Ala                  10 15 20 acc tct gct gat cgt gct gag cag gca gct gaa gaa gca gct gcc cgc 211 Thr Ser Ala Asp Arg Ala Glu Gln Ala Ala Glu Glu Ala Ala Ala Arg              25 30 35 caa gcg gag gaa tct cca ttt gga cag gcc tca gag gaa gaa att tct 259 Gln Ala Glu Glu Ser Pro Phe Gly Gln Ala Ser Glu Glu Glu Ile Ser          40 45 50 cca gag ctc gaa gca gag atc aat gat ctt cta tca gat gtt gat cca 307 Pro Glu Leu Glu Ala Glu Ile Asn Asp Leu Leu Ser Asp Val Asp Pro      55 60 65 gat ttg gat ggc gat ggt gaa gtg tcc gct gta gaa aca cag ctt gcc 355 Asp Leu Asp Gly Asp Gly Glu Val Ser Ala Val Glu Thr Gln Leu Ala  70 75 80 85 gaa cgc act gag gat ctg cag cga gtc acc gct gag tac gcc aac tac 403 Glu Arg Thr Glu Asp Leu Gln Arg Val Thr Ala Glu Tyr Ala Asn Tyr                  90 95 100 cgt cga cgt acc gag cgt gaa cgc cag ggc atc atc gac acc gca cgc 451 Arg Arg Arg Thr Glu Arg Glu Arg Gln Gly Ile Ile Asp Thr Ala Arg             105 110 115 gca ggt gtt gtt acc caa ctt ctg ccg ttg ctc gac gat ctt gac ctg 499 Ala Gly Val Val Thr Gln Leu Leu Pro Leu Leu Asp Asp Leu Asp Leu         120 125 130 gct gaa cag cac ggt gac ctt aac gaa ggt ccg ctg aag tca ctg tct 547 Ala Glu Gln His Gly Asp Leu Asn Glu Gly Pro Leu Lys Ser Leu Ser     135 140 145 gac aag ctg atc aac atc ctg ggt gga ttg aag gtg gaa tcc ttc ggc 595 Asp Lys Leu Ile Asn Ile Leu Gly Gly Leu Lys Val Glu Ser Phe Gly 150 155 160 165 gag atc ggc gaa gca ttc gat cca gag atc cac gaa gca gta cag gat 643 Glu Ile Gly Glu Ala Phe Asp Pro Glu Ile His Glu Ala Val Gln Asp                 170 175 180 ctc tca cag ggt gat gtc aag gtt ctg gga acg gta ctc cgc aag gga 691 Leu Ser Gln Gly Asp Val Lys Val Leu Gly Thr Val Leu Arg Lys Gly             185 190 195 tac cgc ctc ggc gac cgc gtc atc cgc acc gca atg gtc ctc att ggg 739 Tyr Arg Leu Gly Asp Arg Val Ile Arg Thr Ala Met Val Leu Ile Gly         200 205 210 gat cca gag gag agc tagagagact aagtctctta gtg 777 Asp Pro Glu Glu Ser     215 <210> 30 <211> 218 <212> PRT <213> Corynebacterium glutamicum <400> 30 Met Thr Thr Pro Asn Gly Met Pro Asp Asn Pro Gly Asp Pro Glu Asn   1 5 10 15 Thr Asp Pro Glu Ala Thr Ser Ala Asp Arg Ala Glu Gln Ala Ala Glu              20 25 30 Glu Ala Ala Ala Arg Gln Ala Glu Glu Ser Pro Phe Gly Gln Ala Ser          35 40 45 Glu Glu Glu Ile Ser Pro Glu Leu Glu Ala Glu Ile Asn Asp Leu Leu      50 55 60 Ser Asp Val Asp Pro Asp Leu Asp Gly Asp Gly Glu Val Ser Ala Val  65 70 75 80 Glu Thr Gln Leu Ala Glu Arg Thr Glu Asp Leu Gln Arg Val Thr Ala                  85 90 95 Glu Tyr Ala Asn Tyr Arg Arg Arg Thr Glu Arg Glu Arg Gln Gly Ile             100 105 110 Ile Asp Thr Ala Arg Ala Gly Val Val Thr Gln Leu Leu Pro Leu Leu         115 120 125 Asp Asp Leu Asp Leu Ala Glu Gln His Gly Asp Leu Asn Glu Gly Pro     130 135 140 Leu Lys Ser Leu Ser Asp Lys Leu Ile Asn Ile Leu Gly Gly Leu Lys 145 150 155 160 Val Glu Ser Phe Gly Glu Ile Gly Glu Ala Phe Asp Pro Glu Ile His                 165 170 175 Glu Ala Val Gln Asp Leu Ser Gln Gly Asp Val Lys Val Leu Gly Thr             180 185 190 Val Leu Arg Lys Gly Tyr Arg Leu Gly Asp Arg Val Ile Arg Thr Ala         195 200 205 Met Val Leu Ile Gly Asp Pro Glu Glu Ser     210 215 <210> 31 <211> 1977 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1954) <223> RXN02543 <400> 31 ctcaatgagg agtttttctt accggcgaaa gtcggtggga agcaagtcaa agctcaagcc 60 gtggacagta ctaaaatcac ctaaaacagg aggcaccatt atg gga cgt gca gta 115                                             Met Gly Arg Ala Val                                               1 5 gga att gac ctt gga acc acc aac tct gtg gtt tcc gta ctt gaa ggc 163 Gly Ile Asp Leu Gly Thr Thr Asn Ser Val Val Ser Val Leu Glu Gly                  10 15 20 ggc gag cca gta gtt atc gca aac gca gaa ggc tca cgc acc acc cct 211 Gly Glu Pro Val Val Ile Ala Asn Ala Glu Gly Ser Arg Thr Thr Pro              25 30 35 tcc gtc gtt gca ttc gca aag aac ggt gaa gtt cta gtc ggc cag tcc 259 Ser Val Val Ala Phe Ala Lys Asn Gly Glu Val Leu Val Gly Gln Ser          40 45 50 gct aag aac cag gcg gtc acc aac gtt gac cgc acc att cgc tcc gtc 307 Ala Lys Asn Gln Ala Val Thr Asn Val Asp Arg Thr Ile Arg Ser Val      55 60 65 aag cgc cac atc ggc acc gac tgg tcc gtt gct atc gat gac aag aac 355 Lys Arg His Ile Gly Thr Asp Trp Ser Val Ala Ile Asp Asp Lys Asn  70 75 80 85 tac acc tca cag gaa atc tcg gct cgt acc ctg atg aag ctg aag cgc 403 Tyr Thr Ser Gln Glu Ile Ser Ala Arg Thr Leu Met Lys Leu Lys Arg                  90 95 100 gac gct gaa gca tac ctg ggc gag gac gtc act gat gct gtt att acc 451 Asp Ala Glu Ala Tyr Leu Gly Glu Asp Val Thr Asp Ala Val Ile Thr             105 110 115 gtt cct gca tac ttc gag gac tca cag cgc cag gca acc aag gaa gct 499 Val Pro Ala Tyr Phe Glu Asp Ser Gln Arg Gln Ala Thr Lys Glu Ala         120 125 130 ggt cag atc gca ggc ctt aac gtt ctg cgt att gtt aac gag cca acc 547 Gly Gln Ile Ala Gly Leu Asn Val Leu Arg Ile Val Asn Glu Pro Thr     135 140 145 gcg gct gca ctt gca tac ggc ctt gag aag ggc gag cag gag cag acc 595 Ala Ala Ala Leu Ala Tyr Gly Leu Glu Lys Gly Glu Gln Glu Gln Thr 150 155 160 165 att ctg gta ttc gac ctc ggt ggc ggc acc ttc gac gtc tcc ctc cta 643 Ile Leu Val Phe Asp Leu Gly Gly Gly Thr Phe Asp Val Ser Leu Leu                 170 175 180 gag atc ggc gac ggt gtt gtt gag gtt cgc gca acc tcc ggc gat aac 691 Glu Ile Gly Asp Gly Val Val Glu Val Arg Ala Thr Ser Gly Asp Asn             185 190 195 gag ctc ggt ggc gac gac tgg gat cag cgt atc gtt gac tgg ctg gta 739 Glu Leu Gly Gly Asp Asp Trp Asp Gln Arg Ile Val Asp Trp Leu Val         200 205 210 gag aag ttc cag tcc tcc aac ggc att gac ctg acc aag gac aag atg 787 Glu Lys Phe Gln Ser Ser Asn Gly Ile Asp Leu Thr Lys Asp Lys Met     215 220 225 gcc ctg cag cgt ctg cgt gag gca gct gag aag gca aag atc gag ctg 835 Ala Leu Gln Arg Leu Arg Glu Ala Ala Glu Lys Ala Lys Ile Glu Leu 230 235 240 245 tcc tct tcc cag agt gca aac atc aac ctt cct tac atc acc gtt gat 883 Ser Ser Ser Gln Ser Ala Asn Ile Asn Leu Pro Tyr Ile Thr Val Asp                 250 255 260 gca gac aag aac cca ctg ttc ttg gat gag acc ctt tcc cgt gcc gag 931 Ala Asp Lys Asn Pro Leu Phe Leu Asp Glu Thr Leu Ser Arg Ala Glu             265 270 275 ttc cag cgc atc acc cag gac ctc ctg gcc cgc acc aag act cct ttc 979 Phe Gln Arg Ile Thr Gln Asp Leu Leu Ala Arg Thr Lys Thr Pro Phe         280 285 290 aac cag gtt gtt aag gac gct ggc gtg tcc gtc tcg gag atc gac cac 1027 Asn Gln Val Val Lys Asp Ala Gly Val Ser Val Ser Glu Ile Asp His     295 300 305 gtt gtt ctc gtc ggt ggt tcc acc cgt atg cct gct gtt acc gaa ctg 1075 Val Val Leu Val Gly Gly Ser Thr Arg Met Pro Ala Val Thr Glu Leu 310 315 320 325 gtc aag gaa ctg acc ggt gga cgt gag cca aac aag ggt gtt aac cca 1123 Val Lys Glu Leu Thr Gly Gly Arg Glu Pro Asn Lys Gly Val Asn Pro                 330 335 340 gat gag gtt gtt gca gtt ggt gca gca ctt cag gcc ggt gtt ctc cgc 1171 Asp Glu Val Val Ala Val Gly Ala Ala Leu Gln Ala Gly Val Leu Arg             345 350 355 ggc gag gtc aag gat gtt ctt ctt ctt gac gtc acc cca ctg tcc ctc 1219 Gly Glu Val Lys Asp Val Leu Leu Leu Asp Val Thr Pro Leu Ser Leu         360 365 370 ggc att gag acc aag ggt ggc gtg atg acc aag ctc atc gag cgc aac 1267 Gly Ile Glu Thr Lys Gly Gly Val Met Thr Lys Leu Ile Glu Arg Asn     375 380 385 acc acc atc cct acc aag cgt tcc gag acc ttc acc acc gca gag gac 1315 Thr Thr Ile Pro Thr Lys Arg Ser Glu Thr Phe Thr Thr Ala Glu Asp 390 395 400 405 aac cag cct tct gtt cag atc cag gtc ttc cag ggc gag cgt gaa atc 1363 Asn Gln Pro Ser Val Gln Ile Gln Val Phe Gln Gly Glu Arg Glu Ile                 410 415 420 gca acc gcc aac aag ctg ctc gga tcc ttc gag ctc ggc ggc atc gca 1411 Ala Thr Ala Asn Lys Leu Leu Gly Ser Phe Glu Leu Gly Gly Ile Ala             425 430 435 cct gca cca cgt ggc gtc cca cag atc gag gtc act ttc gac atc gac 1459 Pro Ala Pro Arg Gly Val Pro Gln Ile Glu Val Thr Phe Asp Ile Asp         440 445 450 gcc aac ggc atc gtc cac gtc acc gca aag gac aag ggt act ggc aag 1507 Ala Asn Gly Ile Val His Val Thr Ala Lys Asp Lys Gly Thr Gly Lys     455 460 465 gaa aac acc atc acc att cag gac ggc tcc ggt ctc tcc cag gat gaa 1555 Glu Asn Thr Ile Thr Ile Gln Asp Gly Ser Gly Leu Ser Gln Asp Glu 470 475 480 485 att gat cgc atg atc aag gat gct gaa gct cac gct gat gag gac aag 1603 Ile Asp Arg Met Ile Lys Asp Ala Glu Ala His Ala Asp Glu Asp Lys                 490 495 500 aag cgc cgc gag gag cag gaa gtc cgc aac aac gct gag tcc ctg gtt 1651 Lys Arg Arg Glu Glu Gln Glu Val Arg Asn Asn Ala Glu Ser Leu Val             505 510 515 tac cag acc cgc aag ttc gtt gaa gag aac tcc gag aag gtc tcc gaa 1699 Tyr Gln Thr Arg Lys Phe Val Glu Glu Asn Ser Glu Lys Val Ser Glu         520 525 530 gac ctc aag gca aag gtc gaa gag gca gcc aag ggc gtt gaa gaa gca 1747 Asp Leu Lys Ala Lys Val Glu Glu Ala Ala Lys Gly Val Glu Glu Ala     535 540 545 ctc aag ggc gag gac ctc gag gca atc aag gct gca gtt gag aag ctg 1795 Leu Lys Gly Glu Asp Leu Glu Ala Ile Lys Ala Ala Val Glu Lys Leu 550 555 560 565 aac acc gag tcc cag gaa atg ggt aag gct atc tac gag gct gac gct 1843 Asn Thr Glu Ser Gln Glu Met Gly Lys Ala Ile Tyr Glu Ala Asp Ala                 570 575 580 gct gct ggt gca acc cag gct gac gca ggt gca gaa ggc gct gca gat 1891 Ala Ala Gly Ala Thr Gln Ala Asp Ala Gly Ala Glu Gly Ala Ala Asp             585 590 595 gac aat gtt gtt gac gct gaa gtt gtc gaa gac gac gca gct gac aat 1939 Asp Asn Val Val Asp Ala Glu Val Val Glu Asp Asp Ala Ala Asp Asn         600 605 610 ggt gag gac aag aag taaatgacta cccctaacgg aat 1977 Gly Glu Asp Lys Lys     615 <210> 32 <211> 618 <212> PRT <213> Corynebacterium glutamicum <400> 32 Met Gly Arg Ala Val Gly Ile Asp Leu Gly Thr Thr Asn Ser Val Val   1 5 10 15 Ser Val Leu Glu Gly Gly Glu Pro Val Val Ile Ala Asn Ala Glu Gly              20 25 30 Ser Arg Thr Thr Pro Ser Val Val Ala Phe Ala Lys Asn Gly Glu Val          35 40 45 Leu Val Gly Gln Ser Ala Lys Asn Gln Ala Val Thr Asn Val Asp Arg      50 55 60 Thr Ile Arg Ser Val Lys Arg His Ile Gly Thr Asp Trp Ser Val Ala  65 70 75 80 Ile Asp Asp Lys Asn Tyr Thr Ser Gln Glu Ile Ser Ala Arg Thr Leu                  85 90 95 Met Lys Leu Lys Arg Asp Ala Glu Ala Tyr Leu Gly Glu Asp Val Thr             100 105 110 Asp Ala Val Ile Thr Val Pro Ala Tyr Phe Glu Asp Ser Gln Arg Gln         115 120 125 Ala Thr Lys Glu Ala Gly Gln Ile Ala Gly Leu Asn Val Leu Arg Ile     130 135 140 Val Asn Glu Pro Thr Ala Ala Ala Leu Ala Tyr Gly Leu Glu Lys Gly 145 150 155 160 Glu Gln Glu Gln Thr Ile Leu Val Phe Asp Leu Gly Gly Gly Thr Phe                 165 170 175 Asp Val Ser Leu Leu Glu Ile Gly Asp Gly Val Val Glu Val Arg Ala             180 185 190 Thr Ser Gly Asp Asn Glu Leu Gly Gly Asp Asp Trp Asp Gln Arg Ile         195 200 205 Val Asp Trp Leu Val Glu Lys Phe Gln Ser Ser Asn Gly Ile Asp Leu     210 215 220 Thr Lys Asp Lys Met Ala Leu Gln Arg Leu Arg Glu Ala Ala Glu Lys 225 230 235 240 Ala Lys Ile Glu Leu Ser Ser Ser Gln Ser Ala Asn Ile Asn Leu Pro                 245 250 255 Tyr Ile Thr Val Asp Ala Asp Lys Asn Pro Leu Phe Leu Asp Glu Thr             260 265 270 Leu Ser Arg Ala Glu Phe Gln Arg Ile Thr Gln Asp Leu Leu Ala Arg         275 280 285 Thr Lys Thr Pro Phe Asn Gln Val Val Lys Asp Ala Gly Val Ser Val     290 295 300 Ser Glu Ile Asp His Val Val Leu Val Gly Gly Ser Thr Arg Met Pro 305 310 315 320 Ala Val Thr Glu Leu Val Lys Glu Leu Thr Gly Gly Arg Glu Pro Asn                 325 330 335 Lys Gly Val Asn Pro Asp Glu Val Val Ala Val Gly Ala Ala Leu Gln             340 345 350 Ala Gly Val Leu Arg Gly Glu Val Lys Asp Val Leu Leu Leu Asp Val         355 360 365 Thr Pro Leu Ser Leu Gly Ile Glu Thr Lys Gly Gly Val Met Thr Lys     370 375 380 Leu Ile Glu Arg Asn Thr Thr Ile Pro Thr Lys Arg Ser Glu Thr Phe 385 390 395 400 Thr Thr Ala Glu Asp Asn Gln Pro Ser Val Gln Ile Gln Val Phe Gln                 405 410 415 Gly Glu Arg Glu Ile Ala Thr Ala Asn Lys Leu Leu Gly Ser Phe Glu             420 425 430 Leu Gly Gly Ile Ala Pro Ala Pro Arg Gly Val Pro Gln Ile Glu Val         435 440 445 Thr Phe Asp Ile Asp Ala Asn Gly Ile Val His Val Thr Ala Lys Asp     450 455 460 Lys Gly Thr Gly Lys Glu Asn Thr Ile Thr Ile Gln Asp Gly Ser Gly 465 470 475 480 Leu Ser Gln Asp Glu Ile Asp Arg Met Ile Lys Asp Ala Glu Ala His                 485 490 495 Ala Asp Glu Asp Lys Lys Arg Arg Glu Glu Gln Glu Val Arg Asn Asn             500 505 510 Ala Glu Ser Leu Val Tyr Gln Thr Arg Lys Phe Val Glu Glu Asn Ser         515 520 525 Glu Lys Val Ser Glu Asp Leu Lys Ala Lys Val Glu Glu Ala Ala Lys     530 535 540 Gly Val Glu Glu Ala Leu Lys Gly Glu Asp Leu Glu Ala Ile Lys Ala 545 550 555 560 Ala Val Glu Lys Leu Asn Thr Glu Ser Gln Glu Met Gly Lys Ala Ile                 565 570 575 Tyr Glu Ala Asp Ala Ala Ala Gly Ala Thr Gln Ala Asp Ala Gly Ala             580 585 590 Glu Gly Ala Ala Asp Asp Asn Val Val Asp Ala Glu Val Val Glu Asp         595 600 605 Asp Ala Ala Asp Asn Gly Glu Asp Lys Lys     610 615 <210> 33 <211> 1977 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1954) <223> FRXA02543 <220> <221> misc_feature <222> 1824 N = a, t, c, or g <220> <221> misc_feature <222> 1830 N = a, t, c, or g <220> <221> misc_feature <222> 1844 N = a, t, c, or g <220> <221> VARIANT <222> 575 <223> Xaa = Val, Ala, Asp, or Gly <220> <221> VARIANT <222> 577 Xaa = Phe, Ser, Tyr, or Cys <220> <221> VARIANT <222> 582 <223> Xaa = Ser, Pro, Thr, or Ala <400> 33 ctcaatgagg agtttttctt accggcgaaa gtcggtggga agcaagtcaa agctcaagcc 60 gtggacagta ctaaaatcac ctaaaacagg aggcaccatt atg gga cgt gca gta 115                                             Met Gly Arg Ala Val                                               1 5 gga att gac ctt gga acc acc aac tct gtg gtt tcc gta ctt gaa ggc 163 Gly Ile Asp Leu Gly Thr Thr Asn Ser Val Val Ser Val Leu Glu Gly                  10 15 20 ggc gag cca gta gtt atc gca aac gca gaa ggc tca cgc acc acc cct 211 Gly Glu Pro Val Val Ile Ala Asn Ala Glu Gly Ser Arg Thr Thr Pro              25 30 35 tcc gtc gtt gca ttc gca aag aac ggt gaa gtt cta gtc ggc cag tcc 259 Ser Val Val Ala Phe Ala Lys Asn Gly Glu Val Leu Val Gly Gln Ser          40 45 50 gct aag aac cag gcg gtc acc aac gtt gac cgc acc att cgc tcc gtc 307 Ala Lys Asn Gln Ala Val Thr Asn Val Asp Arg Thr Ile Arg Ser Val      55 60 65 aag cgc cac atc ggc acc gac tgg tcc gtt gct atc gat gac aag aac 355 Lys Arg His Ile Gly Thr Asp Trp Ser Val Ala Ile Asp Asp Lys Asn  70 75 80 85 tac acc tca cag gaa atc tcg gct cgt acc ctg atg aag ctg aag cgc 403 Tyr Thr Ser Gln Glu Ile Ser Ala Arg Thr Leu Met Lys Leu Lys Arg                  90 95 100 gac gct gaa gca tac ctg ggc gag gac gtc act gat gct gtt att acc 451 Asp Ala Glu Ala Tyr Leu Gly Glu Asp Val Thr Asp Ala Val Ile Thr             105 110 115 gtt cct gca tac ttc gag gac tca cag cgc cag gca acc aag gaa gct 499 Val Pro Ala Tyr Phe Glu Asp Ser Gln Arg Gln Ala Thr Lys Glu Ala         120 125 130 ggt cag atc gca ggc ctt aac gtt ctg cgt att gtt aac gag cca acc 547 Gly Gln Ile Ala Gly Leu Asn Val Leu Arg Ile Val Asn Glu Pro Thr     135 140 145 gcg gct gca ctt gca tac ggc ctt gag aag ggc gag cag gag cag acc 595 Ala Ala Ala Leu Ala Tyr Gly Leu Glu Lys Gly Glu Gln Glu Gln Thr 150 155 160 165 att ctg gta ttc gac ctc ggt ggc ggc acc ttc gac gtc tcc ctc cta 643 Ile Leu Val Phe Asp Leu Gly Gly Gly Thr Phe Asp Val Ser Leu Leu                 170 175 180 gag atc ggc gac ggt gtt gtt gag gtt cgc gca acc tcc ggc gat aac 691 Glu Ile Gly Asp Gly Val Val Glu Val Arg Ala Thr Ser Gly Asp Asn             185 190 195 gag ctc ggt ggc gac gac tgg gat cag cgt atc gtt gac tgg ctg gta 739 Glu Leu Gly Gly Asp Asp Trp Asp Gln Arg Ile Val Asp Trp Leu Val         200 205 210 gag aag ttc cag tcc tcc aac ggc att gac ctg acc aag gac aag atg 787 Glu Lys Phe Gln Ser Ser Asn Gly Ile Asp Leu Thr Lys Asp Lys Met     215 220 225 gcc ctg cag cgt ctg cgt gag gca gct gag aag gca aag atc gag ctg 835 Ala Leu Gln Arg Leu Arg Glu Ala Ala Glu Lys Ala Lys Ile Glu Leu 230 235 240 245 tcc tct tcc cag agt gca aac atc aac ctt cct tac atc acc gtt gat 883 Ser Ser Ser Gln Ser Ala Asn Ile Asn Leu Pro Tyr Ile Thr Val Asp                 250 255 260 gca gac aag aac cca ctg ttc ttg gat gag acc ctt tcc cgt gcc gag 931 Ala Asp Lys Asn Pro Leu Phe Leu Asp Glu Thr Leu Ser Arg Ala Glu             265 270 275 ttc cag cgc atc acc cag gac ctc ctg gcc cgc acc aag act cct ttc 979 Phe Gln Arg Ile Thr Gln Asp Leu Leu Ala Arg Thr Lys Thr Pro Phe         280 285 290 aac cag gtt gtt aag gac gct ggc gtg tcc gtc tcg gag atc gac cac 1027 Asn Gln Val Val Lys Asp Ala Gly Val Ser Val Ser Glu Ile Asp His     295 300 305 gtt gtt ctc gtc ggt ggt tcc acc cgt atg cct gct gtt acc gaa ctg 1075 Val Val Leu Val Gly Gly Ser Thr Arg Met Pro Ala Val Thr Glu Leu 310 315 320 325 gtc aag gaa ctg acc ggt gga cgt gag cca aac aag ggt gtt aac cca 1123 Val Lys Glu Leu Thr Gly Gly Arg Glu Pro Asn Lys Gly Val Asn Pro                 330 335 340 gat gag gtt gtt gca gtt ggt gca gca ctt cag gcc ggt gtt ctc cgc 1171 Asp Glu Val Val Ala Val Gly Ala Ala Leu Gln Ala Gly Val Leu Arg             345 350 355 ggc gag gtc aag gat gtt ctt ctt ctt gac gtc acc cca ctg tcc ctc 1219 Gly Glu Val Lys Asp Val Leu Leu Leu Asp Val Thr Pro Leu Ser Leu         360 365 370 ggc att gag acc aag ggt ggc gtg atg acc aag ctc atc gag cgc aac 1267 Gly Ile Glu Thr Lys Gly Gly Val Met Thr Lys Leu Ile Glu Arg Asn     375 380 385 acc acc atc cct acc aag cgt tcc gag acc ttc acc acc gca gag gac 1315 Thr Thr Ile Pro Thr Lys Arg Ser Glu Thr Phe Thr Thr Ala Glu Asp 390 395 400 405 aac cag cct tct gtt cag atc cag gtc ttc cag ggc gag cgt gaa atc 1363 Asn Gln Pro Ser Val Gln Ile Gln Val Phe Gln Gly Glu Arg Glu Ile                 410 415 420 gca acc gcc aac aag ctg ctc gga tcc ttc gag ctc ggc ggc atc gca 1411 Ala Thr Ala Asn Lys Leu Leu Gly Ser Phe Glu Leu Gly Gly Ile Ala             425 430 435 cct gca cca cgt ggc gtc cca cag atc gag gtc act ttc gac atc gac 1459 Pro Ala Pro Arg Gly Val Pro Gln Ile Glu Val Thr Phe Asp Ile Asp         440 445 450 gcc aac ggc atc gtc cac gtc acc gca aag gac aag ggt act ggc aag 1507 Ala Asn Gly Ile Val His Val Thr Ala Lys Asp Lys Gly Thr Gly Lys     455 460 465 gaa aac acc atc acc att cag gac ggc tcc ggt ctc tcc cag gat gaa 1555 Glu Asn Thr Ile Thr Ile Gln Asp Gly Ser Gly Leu Ser Gln Asp Glu 470 475 480 485 att gat cgc atg atc aag gat gct gaa gct cac gct gat gag gac aag 1603 Ile Asp Arg Met Ile Lys Asp Ala Glu Ala His Ala Asp Glu Asp Lys                 490 495 500 aag cgc cgc gag gag cag gaa gtc cgc aac aac gct gag tcc ctg gtt 1651 Lys Arg Arg Glu Glu Gln Glu Val Arg Asn Asn Ala Glu Ser Leu Val             505 510 515 tac cag acc cgc aag ttc gtt gaa gag aac tcc gag aag gtc tcc gaa 1699 Tyr Gln Thr Arg Lys Phe Val Glu Glu Asn Ser Glu Lys Val Ser Glu         520 525 530 gac ctc aag gca aag gtc gaa gag gca gcc aag ggc gtt gaa gaa gca 1747 Asp Leu Lys Ala Lys Val Glu Glu Ala Ala Lys Gly Val Glu Glu Ala     535 540 545 ctc aag ggc gag gac ctc gag gca atc aag gct gca gtt gag aag ctg 1795 Leu Lys Gly Glu Asp Leu Glu Ala Ile Lys Ala Ala Val Glu Lys Leu 550 555 560 565 aac acc gag tcc cag gaa atg ggt aag gnt atc tnc gag gct gac gct 1843 Asn Thr Glu Ser Gln Glu Met Gly Lys Xaa Ile Xaa Glu Ala Asp Ala                 570 575 580 nct gct ggt gca acc cag gct gac gca ggt gca gaa ggc gct gca gat 1891 Xaa Ala Gly Ala Thr Gln Ala Asp Ala Gly Ala Glu Gly Ala Ala Asp             585 590 595 gac aat gtt gtt gac gct gaa gtt gtc gaa gac gac gca gct gac aat 1939 Asp Asn Val Val Asp Ala Glu Val Val Glu Asp Asp Ala Ala Asp Asn         600 605 610 ggt gag gac aag aag taaatgacta cccctaacgg aat 1977 Gly Glu Asp Lys Lys     615 <210> 34 <211> 618 <212> PRT <213> Corynebacterium glutamicum <220> <221> VARIANT <222> 575 <223> Xaa = Val, Ala, Asp, or Gly <220> <221> VARIANT <222> 577 Xaa = Phe, Ser, Tyr, or Cys <220> <221> VARIANT <222> 582 <223> Xaa = Ser, Pro, Thr, or Ala <400> 34 Met Gly Arg Ala Val Gly Ile Asp Leu Gly Thr Thr Asn Ser Val Val   1 5 10 15 Ser Val Leu Glu Gly Gly Glu Pro Val Val Ile Ala Asn Ala Glu Gly              20 25 30 Ser Arg Thr Thr Pro Ser Val Val Ala Phe Ala Lys Asn Gly Glu Val          35 40 45 Leu Val Gly Gln Ser Ala Lys Asn Gln Ala Val Thr Asn Val Asp Arg      50 55 60 Thr Ile Arg Ser Val Lys Arg His Ile Gly Thr Asp Trp Ser Val Ala  65 70 75 80 Ile Asp Asp Lys Asn Tyr Thr Ser Gln Glu Ile Ser Ala Arg Thr Leu                  85 90 95 Met Lys Leu Lys Arg Asp Ala Glu Ala Tyr Leu Gly Glu Asp Val Thr             100 105 110 Asp Ala Val Ile Thr Val Pro Ala Tyr Phe Glu Asp Ser Gln Arg Gln         115 120 125 Ala Thr Lys Glu Ala Gly Gln Ile Ala Gly Leu Asn Val Leu Arg Ile     130 135 140 Val Asn Glu Pro Thr Ala Ala Ala Leu Ala Tyr Gly Leu Glu Lys Gly 145 150 155 160 Glu Gln Glu Gln Thr Ile Leu Val Phe Asp Leu Gly Gly Gly Thr Phe                 165 170 175 Asp Val Ser Leu Leu Glu Ile Gly Asp Gly Val Val Glu Val Arg Ala             180 185 190 Thr Ser Gly Asp Asn Glu Leu Gly Gly Asp Asp Trp Asp Gln Arg Ile         195 200 205 Val Asp Trp Leu Val Glu Lys Phe Gln Ser Ser Asn Gly Ile Asp Leu     210 215 220 Thr Lys Asp Lys Met Ala Leu Gln Arg Leu Arg Glu Ala Ala Glu Lys 225 230 235 240 Ala Lys Ile Glu Leu Ser Ser Ser Gln Ser Ala Asn Ile Asn Leu Pro                 245 250 255 Tyr Ile Thr Val Asp Ala Asp Lys Asn Pro Leu Phe Leu Asp Glu Thr             260 265 270 Leu Ser Arg Ala Glu Phe Gln Arg Ile Thr Gln Asp Leu Leu Ala Arg         275 280 285 Thr Lys Thr Pro Phe Asn Gln Val Val Lys Asp Ala Gly Val Ser Val     290 295 300 Ser Glu Ile Asp His Val Val Leu Val Gly Gly Ser Thr Arg Met Pro 305 310 315 320 Ala Val Thr Glu Leu Val Lys Glu Leu Thr Gly Gly Arg Glu Pro Asn                 325 330 335 Lys Gly Val Asn Pro Asp Glu Val Val Ala Val Gly Ala Ala Leu Gln             340 345 350 Ala Gly Val Leu Arg Gly Glu Val Lys Asp Val Leu Leu Leu Asp Val         355 360 365 Thr Pro Leu Ser Leu Gly Ile Glu Thr Lys Gly Gly Val Met Thr Lys     370 375 380 Leu Ile Glu Arg Asn Thr Thr Ile Pro Thr Lys Arg Ser Glu Thr Phe 385 390 395 400 Thr Thr Ala Glu Asp Asn Gln Pro Ser Val Gln Ile Gln Val Phe Gln                 405 410 415 Gly Glu Arg Glu Ile Ala Thr Ala Asn Lys Leu Leu Gly Ser Phe Glu             420 425 430 Leu Gly Gly Ile Ala Pro Ala Pro Arg Gly Val Pro Gln Ile Glu Val         435 440 445 Thr Phe Asp Ile Asp Ala Asn Gly Ile Val His Val Thr Ala Lys Asp     450 455 460 Lys Gly Thr Gly Lys Glu Asn Thr Ile Thr Ile Gln Asp Gly Ser Gly 465 470 475 480 Leu Ser Gln Asp Glu Ile Asp Arg Met Ile Lys Asp Ala Glu Ala His                 485 490 495 Ala Asp Glu Asp Lys Lys Arg Arg Glu Glu Gln Glu Val Arg Asn Asn             500 505 510 Ala Glu Ser Leu Val Tyr Gln Thr Arg Lys Phe Val Glu Glu Asn Ser         515 520 525 Glu Lys Val Ser Glu Asp Leu Lys Ala Lys Val Glu Glu Ala Ala Lys     530 535 540 Gly Val Glu Glu Ala Leu Lys Gly Glu Asp Leu Glu Ala Ile Lys Ala 545 550 555 560 Ala Val Glu Lys Leu Asn Thr Glu Ser Gln Glu Met Gly Lys Xaa Ile                 565 570 575 Xaa Glu Ala Asp Ala Xaa Ala Gly Ala Thr Gln Ala Asp Ala Gly Ala             580 585 590 Glu Gly Ala Ala Asp Asp Asn Val Val Asp Ala Glu Val Val Glu Asp         595 600 605 Asp Ala Ala Asp Asn Gly Glu Asp Lys Lys     610 615 <210> 35 <211> 1947 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1924) <223> RXN02280 <400> 35 cgcgattgcg tcatcgatcg ttgttgcttc catgcgcacc acactatctt tctgcacgcc 60 ctgatgccct gtggattcaa aactgtgctt ttataggcgt atg caa gaa tcc tca 115                                             Met Gln Glu Ser Ser                                               1 5 cgt gat aat ttc caa gtt gac ctc ggc ggc gtt gtt gat ctt ttg agt 163 Arg Asp Asn Phe Gln Val Asp Leu Gly Gly Val Val Asp Leu Leu Ser                  10 15 20 cgc cac att tat tcc ggt ccg agg gtg tat gtg cgt gag ttg ctg cag 211 Arg His Ile Tyr Ser Gly Pro Arg Val Tyr Val Arg Glu Leu Leu Gln              25 30 35 aat gcg gtt gat gct tgt act gca cgt tct gaa cag ggt gag gag ggc 259 Asn Ala Val Asp Ala Cys Thr Ala Arg Ser Glu Gln Gly Glu Glu Gly          40 45 50 tac gag ccg agt att cgt att cgg ccg gtg acc aag gat cgt gcc acg 307 Tyr Glu Pro Ser Ile Arg Ile Arg Pro Val Thr Lys Asp Arg Ala Thr      55 60 65 ttt tca ctg gtt gat aat ggt acg ggc ctg acc gcg cag gag gcg cgg 355 Phe Ser Leu Val Asp Asn Gly Thr Gly Leu Thr Ala Gln Glu Ala Arg  70 75 80 85 gaa ttg ctg gcg acg gtg ggg cgg acg tcg aaa cgc gat gaa ttc ggt 403 Glu Leu Leu Ala Thr Val Gly Arg Thr Ser Lys Arg Asp Glu Phe Gly                  90 95 100 ctg cag cgg gaa ggt cgc ctg ggg caa ttt ggc atc ggg ctg ctt agt 451 Leu Gln Arg Glu Gly Arg Leu Gly Gln Phe Gly Ile Gly Leu Leu Ser             105 110 115 tgt ttc atg gtg gcg gat gag atc acc atg gtg tcg cat gcg gag ggt 499 Cys Phe Met Val Ala Asp Glu Ile Thr Met Val Ser His Ala Glu Gly         120 125 130 gcg tcg gcg att cgg tgg act ggt cat gcg gat ggc acc ttt aac ctg 547 Ala Ser Ala Ile Arg Trp Thr Gly His Ala Asp Gly Thr Phe Asn Leu     135 140 145 gag att ctt ggg gat gac gca acg gat gtc att ccg gtg ggc acg act 595 Glu Ile Leu Gly Asp Asp Ala Thr Asp Val Ile Pro Val Gly Thr Thr 150 155 160 165 gtg cac ctg act ccg cgc cct gat gag cgc acg ttg ctg acg gaa aat 643 Val His Leu Thr Pro Arg Pro Asp Glu Arg Thr Leu Leu Thr Glu Asn                 170 175 180 tcc gtg gtc acc att gct agt aat tat ggc cgc tac ctg ccg att cct 691 Ser Val Val Thr Ile Ala Ser Asn Tyr Gly Arg Tyr Leu Pro Ile Pro             185 190 195 att gtg gtg cag ggt gag aaa aac acc acc atc act aca tcg ccg gtg 739 Ile Val Val Gln Gly Glu Lys Asn Thr Thr Ile Thr Thr Ser Pro Val         200 205 210 ttt gca aag gat act gat cag cag cac agg ctg tat gcc ggc cgg gag 787 Phe Ala Lys Asp Thr Asp Gln Gln His Arg Leu Tyr Ala Gly Arg Glu     215 220 225 cgc ctt ggt aaa act cct ttt gat gtc atc gat ctc acc ggt cct ggc 835 Arg Leu Gly Lys Thr Pro Phe Asp Val Ile Asp Leu Thr Gly Pro Gly 230 235 240 245 atc gag ggt gtg gct tat gta ttg ccg gag gcc cag gct ccg cat atg 883 Ile Glu Gly Val Ala Tyr Val Leu Pro Glu Ala Gln Ala Pro His Met                 250 255 260 tcc agg cgt cac agt att tat gtc aac cgc atg ttg gtc tct gat ggg 931 Ser Arg Arg His Ser Ile Tyr Val Asn Arg Met Leu Val Ser Asp Gly             265 270 275 cct tcc acg gtg ctg ccc aac tgg gcg ttc ttt gtg gaa tgt gaa atc 979 Pro Ser Thr Val Leu Pro Asn Trp Ala Phe Phe Val Glu Cys Glu Ile         280 285 290 aat tca acc gat ttg gaa ccc acc gca tcg cgt gaa gcg ctc atg gat 1027 Asn Ser Thr Asp Leu Glu Pro Thr Ala Ser Arg Glu Ala Leu Met Asp     295 300 305 gac acc gcg ttc gcg gca acc agg gaa cat atc ggt gag tgc att aaa 1075 Asp Thr Ala Phe Ala Ala Thr Arg Glu His Ile Gly Glu Cys Ile Lys 310 315 320 325 tcg tgg ctg att aat ctc gcc atg acc aag cct cac cgc gtg cgg gaa 1123 Ser Trp Leu Ile Asn Leu Ala Met Thr Lys Pro His Arg Val Arg Glu                 330 335 340 ttt act gcg att cat gat ctt gcc ctg cgc gag ctg tgc caa tcg gac 1171 Phe Thr Ala Ile His Asp Leu Ala Leu Arg Glu Leu Cys Gln Ser Asp             345 350 355 gcg gac ctg gct gaa acc atg ttg ggt ctt ctc acc ttg gag acc tcc 1219 Ala Asp Leu Ala Glu Thr Met Leu Gly Leu Leu Thr Leu Glu Thr Ser         360 365 370 cgt ggt cgc atc tcg atc ggt gag atc acc acg ttg tcc atc acc gag 1267 Arg Gly Arg Ile Ser Ile Gly Glu Ile Thr Thr Leu Ser Ile Thr Glu     375 380 385 gat gtg tcg ctg cag ctg gct acc acg ttg gat gat ttc agg cag ctc 1315 Asp Val Ser Leu Gln Leu Ala Thr Thr Leu Asp Asp Phe Arg Gln Leu 390 395 400 405 aac acc att gcg cgc ccg gac acc ttg att att aat ggc ggc tac att 1363 Asn Thr Ile Ala Arg Pro Asp Thr Leu Ile Ile Asn Gly Gly Tyr Ile                 410 415 420 cac gac agc gat ctg gct cgg ctc att ccc gtt cac tac cca ccg ctt 1411 His Asp Ser Asp Leu Ala Arg Leu Ile Pro Val His Tyr Pro Pro Leu             425 430 435 acg gta tct act gct gac ctg cgc gaa tcc atg gat ctg atg gag ctt 1459 Thr Val Ser Thr Ala Asp Leu Arg Glu Ser Met Asp Leu Met Glu Leu         440 445 450 ccg ccg ctg cag gac att gag aaa gcc aag gca ctg gat gcg cag gtc 1507 Pro Pro Leu Gln Asp Ile Glu Lys Ala Lys Ala Leu Asp Ala Gln Val     455 460 465 acg gaa tca ttg aag gat ttt cag atc aag ggc gca acg agg gtt ttt 1555 Thr Glu Ser Leu Lys Asp Phe Gln Ile Lys Gly Ala Thr Arg Val Phe 470 475 480 485 gaa ccc gca gat gtt cct gcc gtg gtg atc att gat tcc aag gcg cag 1603 Glu Pro Ala Asp Val Pro Ala Val Val Ile Ile Asp Ser Lys Ala Gln                 490 495 500 gcc tca cgg gat cgc aat gaa aca caa agc gca acc act gat cgt tgg 1651 Ala Ser Arg Asp Arg Asn Glu Thr Gln Ser Ala Thr Thr Asp Arg Trp             505 510 515 gct gac att ttg gca acg gtg gat aac acg ttg agc cgt caa aca gcc 1699 Ala Asp Ile Leu Ala Thr Val Asp Asn Thr Leu Ser Arg Gln Thr Ala         520 525 530 aac att cca cag gat cag gga ctg tcg gcg ttg tgc ttg aat tgg aac 1747 Asn Ile Pro Gln Asp Gln Gly Leu Ser Ala Leu Cys Leu Asn Trp Asn     535 540 545 aat tcg ctg gtc agg aaa ttg gcg tcc act gat gac acc gcc gtg gtg 1795 Asn Ser Leu Val Arg Lys Leu Ala Ser Thr Asp Asp Thr Ala Val Val 550 555 560 565 tcg cgc acg gtg cgt ttg ctc tac gtt cag gca ttg ttg tcc agc aag 1843 Ser Arg Thr Val Arg Leu Leu Tyr Val Gln Ala Leu Leu Ser Ser Lys                 570 575 580 agg cca ctg cgg gtg aag gaa cgc gcg ctg ctt aat gat tcg ctg gca 1891 Arg Pro Leu Arg Val Lys Glu Arg Ala Leu Leu Asn Asp Ser Leu Ala             585 590 595 gat ctg gtt tct ttg tct ttg tca tcc gat atc taagacaatc ctccgctaat 1944 Asp Leu Val Ser Leu Ser Leu Ser Ser Asp Ile         600 605 ctt 1947 <210> 36 <211> 608 <212> PRT <213> Corynebacterium glutamicum <400> 36 Met Gln Glu Ser Ser Arg Asp Asn Phe Gln Val Asp Leu Gly Gly Val   1 5 10 15 Val Asp Leu Leu Ser Arg His Ile Tyr Ser Gly Pro Arg Val Tyr Val              20 25 30 Arg Glu Leu Leu Gln Asn Ala Val Asp Ala Cys Thr Ala Arg Ser Glu          35 40 45 Gln Gly Glu Glu Gly Tyr Glu Pro Ser Ile Arg Ile Arg Pro Val Thr      50 55 60 Lys Asp Arg Ala Thr Phe Ser Leu Val Asp Asn Gly Thr Gly Leu Thr  65 70 75 80 Ala Gln Glu Ala Arg Glu Leu Leu Ala Thr Val Gly Arg Thr Ser Lys                  85 90 95 Arg Asp Glu Phe Gly Leu Gln Arg Glu Gly Arg Leu Gly Gln Phe Gly             100 105 110 Ile Gly Leu Leu Ser Cys Phe Met Val Ala Asp Glu Ile Thr Met Val         115 120 125 Ser His Ala Glu Gly Ala Ser Ala Ile Arg Trp Thr Gly His Ala Asp     130 135 140 Gly Thr Phe Asn Leu Glu Ile Leu Gly Asp Asp Ala Thr Asp Val Ile 145 150 155 160 Pro Val Gly Thr Thr Val His Leu Thr Pro Arg Pro Asp Glu Arg Thr                 165 170 175 Leu Leu Thr Glu Asn Ser Val Val Thr Ile Ala Ser Asn Tyr Gly Arg             180 185 190 Tyr Leu Pro Ile Pro Ile Val Val Gln Gly Glu Lys Asn Thr Thr Ile         195 200 205 Thr Thr Ser Pro Val Phe Ala Lys Asp Thr Asp Gln Gln His Arg Leu     210 215 220 Tyr Ala Gly Arg Glu Arg Leu Gly Lys Thr Pro Phe Asp Val Ile Asp 225 230 235 240 Leu Thr Gly Pro Gly Ile Glu Gly Val Ala Tyr Val Leu Pro Glu Ala                 245 250 255 Gln Ala Pro His Met Ser Arg Arg His Ser Ile Tyr Val Asn Arg Met             260 265 270 Leu Val Ser Asp Gly Pro Ser Thr Val Leu Pro Asn Trp Ala Phe Phe         275 280 285 Val Glu Cys Glu Ile Asn Ser Thr Asp Leu Glu Pro Thr Ala Ser Arg     290 295 300 Glu Ala Leu Met Asp Asp Thr Ala Phe Ala Ala Thr Arg Glu His Ile 305 310 315 320 Gly Glu Cys Ile Lys Ser Trp Leu Ile Asn Leu Ala Met Thr Lys Pro                 325 330 335 His Arg Val Arg Glu Phe Thr Ala Ile His Asp Leu Ala Leu Arg Glu             340 345 350 Leu Cys Gln Ser Asp Ala Asp Leu Ala Glu Thr Met Leu Gly Leu Leu         355 360 365 Thr Leu Glu Thr Ser Arg Gly Arg Ile Ser Ile Gly Glu Ile Thr Thr     370 375 380 Leu Ser Ile Thr Glu Asp Val Ser Leu Gln Leu Ala Thr Thr Leu Asp 385 390 395 400 Asp Phe Arg Gln Leu Asn Thr Ile Ala Arg Pro Asp Thr Leu Ile Ile                 405 410 415 Asn Gly Gly Tyr Ile His Asp Ser Asp Leu Ala Arg Leu Ile Pro Val             420 425 430 His Tyr Pro Pro Leu Thr Val Ser Thr Ala Asp Leu Arg Glu Ser Met         435 440 445 Asp Leu Met Glu Leu Pro Pro Leu Gln Asp Ile Glu Lys Ala Lys Ala     450 455 460 Leu Asp Ala Gln Val Thr Glu Ser Leu Lys Asp Phe Gln Ile Lys Gly 465 470 475 480 Ala Thr Arg Val Phe Glu Pro Ala Asp Val Pro Ala Val Val Ile Ile                 485 490 495 Asp Ser Lys Ala Gln Ala Ser Arg Asp Arg Asn Glu Thr Gln Ser Ala             500 505 510 Thr Thr Asp Arg Trp Ala Asp Ile Leu Ala Thr Val Asp Asn Thr Leu         515 520 525 Ser Arg Gln Thr Ala Asn Ile Pro Gln Asp Gln Gly Leu Ser Ala Leu     530 535 540 Cys Leu Asn Trp Asn Asn Ser Leu Val Arg Lys Leu Ala Ser Thr Asp 545 550 555 560 Asp Thr Ala Val Val Ser Arg Thr Val Arg Leu Leu Tyr Val Gln Ala                 565 570 575 Leu Leu Ser Ser Lys Arg Pro Leu Arg Val Lys Glu Arg Ala Leu Leu             580 585 590 Asn Asp Ser Leu Ala Asp Leu Val Ser Leu Ser Leu Ser Ser Asp Ile         595 600 605 <210> 37 <211> 436 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (101) .. (436) <223> FRXA02282 <400> 37 cgcgattgcg tcatcgatcg ttgttgcttc catgcgcacc acactatctt tctgcacgcc 60 ctgatgccct gtggattcaa aactgtgctt ttataggcgt atg caa gaa tcc tca 115                                             Met Gln Glu Ser Ser                                               1 5 cgt gat aat ttc caa gtt gac ctc ggc ggc gtt gtt gat ctt ttg agt 163 Arg Asp Asn Phe Gln Val Asp Leu Gly Gly Val Val Asp Leu Leu Ser                  10 15 20 cgc cac att tat tcc ggt ccg agg gtg tat gtg cgt gag ttg ctg cag 211 Arg His Ile Tyr Ser Gly Pro Arg Val Tyr Val Arg Glu Leu Leu Gln              25 30 35 aat gcg gtt gat gct tgt act gca cgt tct gaa cag ggt gag gag ggc 259 Asn Ala Val Asp Ala Cys Thr Ala Arg Ser Glu Gln Gly Glu Glu Gly          40 45 50 tac gag ccg agt att cgt att cgg ccg gtg acc aag gat cgt gcc acg 307 Tyr Glu Pro Ser Ile Arg Ile Arg Pro Val Thr Lys Asp Arg Ala Thr      55 60 65 ttt tca ctg gtt gat aat ggt acg ggc ctg acc gcg cag gag gcg cgg 355 Phe Ser Leu Val Asp Asn Gly Thr Gly Leu Thr Ala Gln Glu Ala Arg  70 75 80 85 gaa ttg ctg gcg acg gtg ggg cgg acg tcg aaa cgc gat gaa ttc ggt 403 Glu Leu Leu Ala Thr Val Gly Arg Thr Ser Lys Arg Asp Glu Phe Gly                  90 95 100 ctg cag cgg gaa ggt cgc ctg ggg caa ttt ggc 436 Leu Gln Arg Glu Gly Arg Leu Gly Gln Phe Gly             105 110 <210> 38 <211> 112 <212> PRT <213> Corynebacterium glutamicum <400> 38 Met Gln Glu Ser Ser Arg Asp Asn Phe Gln Val Asp Leu Gly Gly Val   1 5 10 15 Val Asp Leu Leu Ser Arg His Ile Tyr Ser Gly Pro Arg Val Tyr Val              20 25 30 Arg Glu Leu Leu Gln Asn Ala Val Asp Ala Cys Thr Ala Arg Ser Glu          35 40 45 Gln Gly Glu Glu Gly Tyr Glu Pro Ser Ile Arg Ile Arg Pro Val Thr      50 55 60 Lys Asp Arg Ala Thr Phe Ser Leu Val Asp Asn Gly Thr Gly Leu Thr  65 70 75 80 Ala Gln Glu Ala Arg Glu Leu Leu Ala Thr Val Gly Arg Thr Ser Lys                  85 90 95 Arg Asp Glu Phe Gly Leu Gln Arg Glu Gly Arg Leu Gly Gln Phe Gly             100 105 110 <210> 39 <211> 1269 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1246) <223> RXA00886 <400> 39 taagtatgtt ggtcgtgtgc tcgctggcga atagctgcgg gtatagttgg ccatttgttt 60 tgattaatct gtttagaagc taaaggaagt atcacccacc gtg gca cgt gac tat 115                                             Val Ala Arg Asp Tyr                                               1 5 tac ggc att ctc ggc gtc gat cgc aat gca acc gaa tca gag atc aaa 163 Tyr Gly Ile Leu Gly Val Asp Arg Asn Ala Thr Glu Ser Glu Ile Lys                  10 15 20 aag gca tac cga aag ctt gcc cgc aaa tac cac ccg gac gta aac cca 211 Lys Ala Tyr Arg Lys Leu Ala Arg Lys Tyr His Pro Asp Val Asn Pro              25 30 35 ggt gag gaa gca gcg gag aaa ttc cgc gag gct tct gtt gcg cat gag 259 Gly Glu Glu Ala Ala Glu Lys Phe Arg Glu Ala Ser Val Ala His Glu          40 45 50 gta ctc act gat ccg gat aag cgc cgc att gtt gat atg ggc ggt gac 307 Val Leu Thr Asp Pro Asp Lys Arg Arg Ile Val Asp Met Gly Gly Asp      55 60 65 cca atg gag caa ggc ggc gga gct ggc gct ggt ggc ttc ggt gga ggc 355 Pro Met Glu Gln Gly Gly Gly Ala Gly Ala Gly Gly Phe Gly Gly Gly  70 75 80 85 ttc ggc ggc agc ggt gga ctg ggc gat atc ttc gat gcc ttc ttc ggc 403 Phe Gly Gly Ser Gly Gly Leu Gly Asp Ile Phe Asp Ala Phe Phe Gly                  90 95 100 ggt ggc gcg ggc ggt tcc cgt gga cca cgt tcc cgc gtg cag cca ggc 451 Gly Gly Ala Gly Gly Ser Arg Gly Pro Arg Ser Arg Val Gln Pro Gly             105 110 115 agt gac acc ttg tgg cgc acc tcc atc acc ttg gaa gag gct tac aag 499 Ser Asp Thr Leu Trp Arg Thr Ser Ile Thr Leu Glu Glu Ala Tyr Lys         120 125 130 ggc gct aag aaa gat ctc acc ctt gac acc gca gtg ctg tgt acc aag 547 Gly Ala Lys Lys Asp Leu Thr Leu Asp Thr Ala Val Leu Cys Thr Lys     135 140 145 tgt cat ggt tct gga tct gca tcc gac aag aag cct gtt acc tgt ggc 595 Cys His Gly Ser Gly Ser Ala Ser Asp Lys Lys Pro Val Thr Cys Gly 150 155 160 165 acc tgt aat ggc gct ggt gaa att cag gaa gtg cag cgc agc ttc ctg 643 Thr Cys Asn Gly Ala Gly Glu Ile Gln Glu Val Gln Arg Ser Phe Leu                 170 175 180 ggc aac gtc atg acg tcc cgc cca tgc cac acc tgc gat ggc acc ggt 691 Gly Asn Val Met Thr Ser Arg Pro Cys His Thr Cys Asp Gly Thr Gly             185 190 195 gag atc atc cca gat cct tgc act gag tgt gca gca gat ggt cgt gtg 739 Glu Ile Ile Pro Asp Pro Cys Thr Glu Cys Ala Ala Asp Gly Arg Val         200 205 210 cgt gct cgc cgc gac atc gtg gcc aac atc cca gct ggc atc cag tcc 787 Arg Ala Arg Arg Asp Ile Val Ala Asn Ile Pro Ala Gly Ile Gln Ser     215 220 225 ggc atg cgc atc cgc atg gca ggc caa ggt gag gtt ggc gct ggt ggc 835 Gly Met Arg Ile Arg Met Ala Gly Gln Gly Glu Val Gly Ala Gly Gly 230 235 240 245 ggt cct gcg ggt gac ctc tac att gaa gtc atg gtg cgc ccg cac gcc 883 Gly Pro Ala Gly Asp Leu Tyr Ile Glu Val Met Val Arg Pro His Ala                 250 255 260 atc ttc acc cgc gat ggc gac gat ctg cac gcc agc atc aag gtt cca 931 Ile Phe Thr Arg Asp Gly Asp Asp Leu His Ala Ser Ile Lys Val Pro             265 270 275 atg ttc gat gca gcg ctt ggc acc gaa ttg gac gtg gaa tcc ctc acc 979 Met Phe Asp Ala Ala Leu Gly Thr Glu Leu Asp Val Glu Ser Leu Thr         280 285 290 ggc gaa gag gtg aaa att acc atc cct gca ggt act cag ccc aac gat 1027 Gly Glu Glu Val Lys Ile Thr Ile Pro Ala Gly Thr Gln Pro Asn Asp     295 300 305 gtg atc acc ttg gat ggt gaa ggc atg ccg aag ctg cgc gca gaa ggc 1075 Val Ile Thr Leu Asp Gly Glu Gly Met Pro Lys Leu Arg Ala Glu Gly 310 315 320 325 cac ggc aac ctc atg gcg cat gtc gat cta ttt gtg cca acc gat ttg 1123 His Gly Asn Leu Met Ala His Val Asp Leu Phe Val Pro Thr Asp Leu                 330 335 340 gat gac cgc acc cgc gaa ttg ctt gaa gaa atc cgc aac cat cgc agc 1171 Asp Asp Arg Thr Arg Glu Leu Leu Glu Glu Ile Arg Asn His Arg Ser             345 350 355 gac aac gct tcc gtg cat cgc gaa ggc gga gaa gaa tcc ggt ttc ttt 1219 Asp Asn Ala Ser Val His Arg Glu Gly Gly Glu Glu Ser Gly Phe Phe         360 365 370 gac aag ctc cga aac aag ttc cgc aaa taatgtcact gccagtattt 1266 Asp Lys Leu Arg Asn Lys Phe Arg Lys     375 380 atc 1269 <210> 40 <211> 382 <212> PRT <213> Corynebacterium glutamicum <400> 40 Val Ala Arg Asp Tyr Tyr Gly Ile Leu Gly Val Asp Arg Asn Ala Thr   1 5 10 15 Glu Ser Glu Ile Lys Lys Ala Tyr Arg Lys Leu Ala Arg Lys Tyr His              20 25 30 Pro Asp Val Asn Pro Gly Glu Glu Ala Ala Glu Lys Phe Arg Glu Ala          35 40 45 Ser Val Ala His Glu Val Leu Thr Asp Pro Asp Lys Arg Arg Ile Val      50 55 60 Asp Met Gly Gly Asp Pro Met Glu Gln Gly Gly Gly Ala Gly Ala Gly  65 70 75 80 Gly Phe Gly Gly Gly Phe Gly Gly Ser Gly Gly Leu Gly Asp Ile Phe                  85 90 95 Asp Ala Phe Phe Gly Gly Gly Ala Gly Gly Ser Arg Gly Pro Arg Ser             100 105 110 Arg Val Gln Pro Gly Ser Asp Thr Leu Trp Arg Thr Ser Ile Thr Leu         115 120 125 Glu Glu Ala Tyr Lys Gly Ala Lys Lys Asp Leu Thr Leu Asp Thr Ala     130 135 140 Val Leu Cys Thr Lys Cys His Gly Ser Gly Ser Ala Ser Asp Lys Lys 145 150 155 160 Pro Val Thr Cys Gly Thr Cys Asn Gly Ala Gly Glu Ile Gln Glu Val                 165 170 175 Gln Arg Ser Phe Leu Gly Asn Val Met Thr Ser Arg Pro Cys His Thr             180 185 190 Cys Asp Gly Thr Gly Glu Ile Ile Pro Asp Pro Cys Thr Glu Cys Ala         195 200 205 Ala Asp Gly Arg Val Arg Ala Arg Arg Asp Ile Val Ala Asn Ile Pro     210 215 220 Ala Gly Ile Gln Ser Gly Met Arg Ile Arg Met Ala Gly Gln Gly Glu 225 230 235 240 Val Gly Ala Gly Gly Gly Pro Ala Gly Asp Leu Tyr Ile Glu Val Met                 245 250 255 Val Arg Pro His Ala Ile Phe Thr Arg Asp Gly Asp Asp Leu His Ala             260 265 270 Ser Ile Lys Val Pro Met Phe Asp Ala Ala Leu Gly Thr Glu Leu Asp         275 280 285 Val Glu Ser Leu Thr Gly Glu Glu Val Lys Ile Thr Ile Pro Ala Gly     290 295 300 Thr Gln Pro Asn Asp Val Ile Thr Leu Asp Gly Glu Gly Met Pro Lys 305 310 315 320 Leu Arg Ala Glu Gly His Gly Asn Leu Met Ala His Val Asp Leu Phe                 325 330 335 Val Pro Thr Asp Leu Asp Asp Arg Thr Arg Glu Leu Leu Glu Glu Ile             340 345 350 Arg Asn His Arg Ser Asp Asn Ala Ser Val His Arg Glu Gly Gly Glu         355 360 365 Glu Ser Gly Phe Phe Asp Lys Leu Arg Asn Lys Phe Arg Lys     370 375 380 <210> 41 <211> 1470 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1447) <223> RXS00568 <400> 41 gtttcggacg acgcgagaaa tcgcattaat cgtcggaaac cgggcttgtt tttgtaatat 60 ctgaaacttt ccctttcccg atcatccagg agatttactc gtg aag agt tct gtc 115                                             Val Lys Ser Ser Val                                               1 5 gag aag ctg agc gac acc cgt tca aag atc acc gtt gag gtt cca ttt 163 Glu Lys Leu Ser Asp Thr Arg Ser Lys Ile Thr Val Glu Val Pro Phe                  10 15 20 tct gaa ctg aag cca gag atc gac cag gca tac gcc gct cta gcg cag 211 Ser Glu Leu Lys Pro Glu Ile Asp Gln Ala Tyr Ala Ala Leu Ala Gln              25 30 35 caa gtc cag atc cct ggt ttc cgt aag ggc aag gca ccg cgt cag ctt 259 Gln Val Gln Ile Pro Gly Phe Arg Lys Gly Lys Ala Pro Arg Gln Leu          40 45 50 atc gac gca cgc ttc ggc cgt ggt gcg gtt ctg gag cag gtt gtc aac 307 Ile Asp Ala Arg Phe Gly Arg Gly Ala Val Leu Glu Gln Val Val Asn      55 60 65 gac atg ctt cct aac cgc tac gca cag gca atc gaa gct gag ggc atc 355 Asp Met Leu Pro Asn Arg Tyr Ala Gln Ala Ile Glu Ala Glu Gly Ile  70 75 80 85 aag gca atc ggc cag cct aac gta gag gtc acc aag atc gaa gac aac 403 Lys Ala Ile Gly Gln Pro Asn Val Glu Val Thr Lys Ile Glu Asp Asn                  90 95 100 gag ctc gtt gag ttc gtc gct gag gtt gac gtt cgc cca gag ttc gag 451 Glu Leu Val Glu Phe Val Ala Glu Val Asp Val Arg Pro Glu Phe Glu             105 110 115 ctt cct aag ttc gag gac atc act gtt gag gtc cca gct atc aag gct 499 Leu Pro Lys Phe Glu Asp Ile Thr Val Glu Val Pro Ala Ile Lys Ala         120 125 130 gac gaa gag gca atc gaa gca gag ctc gag acc ctg cgt gca cgt ttc 547 Asp Glu Glu Ala Ile Glu Ala Glu Leu Glu Thr Leu Arg Ala Arg Phe     135 140 145 tcc acc ttg aag gat cac aac cac aag ctg aag aag ggt gag ttc gtc 595 Ser Thr Leu Lys Asp His Asn His Lys Leu Lys Lys Gly Glu Phe Val 150 155 160 165 acc atc aac atc acc gca agc att gac ggt gag aag att gaa gag gca 643 Thr Ile Asn Ile Thr Ala Ser Ile Asp Gly Glu Lys Ile Glu Glu Ala                 170 175 180 acc act gag ggt ctg tcc tac gaa atc gga tct gac gat ctg att gac 691 Thr Thr Glu Gly Leu Ser Tyr Glu Ile Gly Ser Asp Asp Leu Ile Asp             185 190 195 ggc ctg gac aag gct ctg atc ggc gct aag aag gat gaa acc gta gag 739 Gly Leu Asp Lys Ala Leu Ile Gly Ala Lys Lys Asp Glu Thr Val Glu         200 205 210 ttc acc tct gag ctg gca aac ggc gag cac aag ggc aag gaa gct caa 787 Phe Thr Ser Glu Leu Ala Asn Gly Glu His Lys Gly Lys Glu Ala Gln     215 220 225 atc agc gtt gag atc acc gca acc aag cag cgc gag ctg cct gag ctg 835 Ile Ser Val Glu Ile Thr Ala Thr Lys Gln Arg Glu Leu Pro Glu Leu 230 235 240 245 gat gat gag ttc gca cag ctg gct tct gag ttc gac acc atc gaa gag 883 Asp Asp Glu Phe Ala Gln Leu Ala Ser Glu Phe Asp Thr Ile Glu Glu                 250 255 260 ctt cgt gag tcc acc gtg tct gac gtt gag gct aag cag aag aac gag 931 Leu Arg Glu Ser Thr Val Ser Asp Val Glu Ala Lys Gln Lys Asn Glu             265 270 275 cag gct gct gca atc cgc gac gaa gtt ctc gct gcg gct ctt ggc gag 979 Gln Ala Ala Ala Ile Arg Asp Glu Val Leu Ala Ala Ala Leu Gly Glu         280 285 290 gct gac ttc gct ctg cca cag tcc atc gtt gac gag cag gca cac tcc 1027 Ala Asp Phe Ala Leu Pro Gln Ser Ile Val Asp Glu Gln Ala His Ser     295 300 305 cag ctg cac cag ctc ctc ggc gag ctt gca cac gac gat gct gca ctg 1075 Gln Leu His Gln Leu Leu Gly Glu Leu Ala His Asp Asp Ala Ala Leu 310 315 320 325 aac tcc ctc ctt gag gct cag ggc acc act cgt gaa gag ttc gac aag 1123 Asn Ser Leu Leu Glu Ala Gln Gly Thr Thr Arg Glu Glu Phe Asp Lys                 330 335 340 aag aac gtc gaa gat gct gag aag gct gtt cgc acc cag ctg ttc ctg 1171 Lys Asn Val Glu Asp Ala Glu Lys Ala Val Arg Thr Gln Leu Phe Leu             345 350 355 gac acc ctc tct gag gtt gag gag cct gag gtt tcc cag cag gag ctc 1219 Asp Thr Leu Ser Glu Val Glu Glu Pro Glu Val Ser Gln Gln Glu Leu         360 365 370 acc gac cac atc ctg ttc acc gca cag tct tac ggc atg gac cca aac 1267 Thr Asp His Ile Leu Phe Thr Ala Gln Ser Tyr Gly Met Asp Pro Asn     375 380 385 cag ttc atc ggt cag ctg cag cag tcc ggc cag atc gcg aac ctc ttc 1315 Gln Phe Ile Gly Gln Leu Gln Gln Ser Gly Gln Ile Ala Asn Leu Phe 390 395 400 405 tcc gac gtt cgc cgt ggc aag gct ctt gca cag gct atc tgc cgc gta 1363 Ser Asp Val Arg Arg Gly Lys Ala Leu Ala Gln Ala Ile Cys Arg Val                 410 415 420 aac gtg aag gac tcc gag ggt aac gag atc gac cct aag gaa tac ttc 1411 Asn Val Lys Asp Ser Glu Gly Asn Glu Ile Asp Pro Lys Glu Tyr Phe             425 430 435 ggt gaa gaa gaa gta gct gag act gag tct gaa gct taaaaacttt 1457 Gly Glu Glu Glu Val Ala Glu Thr Glu Ser Glu Ala         440 445 aaagaaataa cgc 1470 <210> 42 <211> 449 <212> PRT <213> Corynebacterium glutamicum <400> 42 Val Lys Ser Ser Val Glu Lys Leu Ser Asp Thr Arg Ser Lys Ile Thr   1 5 10 15 Val Glu Val Pro Phe Ser Glu Leu Lys Pro Glu Ile Asp Gln Ala Tyr              20 25 30 Ala Ala Leu Ala Gln Gln Val Gln Ile Pro Gly Phe Arg Lys Gly Lys          35 40 45 Ala Pro Arg Gln Leu Ile Asp Ala Arg Phe Gly Arg Gly Ala Val Leu      50 55 60 Glu Gln Val Val Asn Asp Met Leu Pro Asn Arg Tyr Ala Gln Ala Ile  65 70 75 80 Glu Ala Glu Gly Ile Lys Ala Ile Gly Gln Pro Asn Val Glu Val Thr                  85 90 95 Lys Ile Glu Asp Asn Glu Leu Val Glu Phe Val Ala Glu Val Asp Val             100 105 110 Arg Pro Glu Phe Glu Leu Pro Lys Phe Glu Asp Ile Thr Val Glu Val         115 120 125 Pro Ala Ile Lys Ala Asp Glu Glu Ala Ile Glu Ala Glu Leu Glu Thr     130 135 140 Leu Arg Ala Arg Phe Ser Thr Leu Lys Asp His Asn His Lys Leu Lys 145 150 155 160 Lys Gly Glu Phe Val Thr Ile Asn Ile Thr Ala Ser Ile Asp Gly Glu                 165 170 175 Lys Ile Glu Glu Ala Thr Thr Glu Gly Leu Ser Tyr Glu Ile Gly Ser             180 185 190 Asp Asp Leu Ile Asp Gly Leu Asp Lys Ala Leu Ile Gly Ala Lys Lys         195 200 205 Asp Glu Thr Val Glu Phe Thr Ser Glu Leu Ala Asn Gly Glu His Lys     210 215 220 Gly Lys Glu Ala Gln Ile Ser Val Glu Ile Thr Ala Thr Lys Gln Arg 225 230 235 240 Glu Leu Pro Glu Leu Asp Asp Glu Phe Ala Gln Leu Ala Ser Glu Phe                 245 250 255 Asp Thr Ile Glu Glu Leu Arg Glu Ser Thr Val Ser Asp Val Glu Ala             260 265 270 Lys Gln Lys Asn Glu Gln Ala Ala Ala Ile Arg Asp Glu Val Leu Ala         275 280 285 Ala Ala Leu Gly Glu Ala Asp Phe Ala Leu Pro Gln Ser Ile Val Asp     290 295 300 Glu Gln Ala His Ser Gln Leu His Gln Leu Leu Gly Glu Leu Ala His 305 310 315 320 Asp Asp Ala Ala Leu Asn Ser Leu Leu Glu Ala Gln Gly Thr Thr Arg                 325 330 335 Glu Glu Phe Asp Lys Lys Asn Val Glu Asp Ala Glu Lys Ala Val Arg             340 345 350 Thr Gln Leu Phe Leu Asp Thr Leu Ser Glu Val Glu Glu Pro Glu Val         355 360 365 Ser Gln Gln Glu Leu Thr Asp His Ile Leu Phe Thr Ala Gln Ser Tyr     370 375 380 Gly Met Asp Pro Asn Gln Phe Ile Gly Gln Leu Gln Gln Ser Gly Gln 385 390 395 400 Ile Ala Asn Leu Phe Ser Asp Val Arg Arg Gly Lys Ala Leu Ala Gln                 405 410 415 Ala Ile Cys Arg Val Asn Val Lys Asp Ser Glu Gly Asn Glu Ile Asp             420 425 430 Pro Lys Glu Tyr Phe Gly Glu Glu Glu Val Ala Glu Thr Glu Ser Glu         435 440 445 Ala      <210> 43 <211> 826 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (826) <223> RXN03038 <400> 43 gcgcggaaaa caccaagtaa gccttacagt ccgacagcct catagcggat gggataagtt 60 ccaaacacgt tcaaatccgt taaagtgcct gtttaaaact atg cat tca aag gaa 115                                             Met His Ser Lys Glu                                               1 5 gag tta aca gtg cgt aaa gga att tcc cgc gtc ctc tcg gta gcg gtt 163 Glu Leu Thr Val Arg Lys Gly Ile Ser Arg Val Leu Ser Val Ala Val                  10 15 20 gct agt tca atc gga ttc gga act gta ctg aca ggc acc ggc atc gca 211 Ala Ser Ser Ile Gly Phe Gly Thr Val Leu Thr Gly Thr Gly Ile Ala              25 30 35 gca gct caa gac tct gca ttt gac tac ggt atg gat cca aac atg aac 259 Ala Ala Gln Asp Ser Ala Phe Asp Tyr Gly Met Asp Pro Asn Met Asn          40 45 50 tac aac ccg atc gat gac atc aag gat cgt ccc gaa gga ttg tcc aat 307 Tyr Asn Pro Ile Asp Asp Ile Lys Asp Arg Pro Glu Gly Leu Ser Asn      55 60 65 ctt ccc tac ttc gga agt aaa ttg acc agc tgg ggc tca tca tat gcc 355 Leu Pro Tyr Phe Gly Ser Lys Leu Thr Ser Trp Gly Ser Ser Tyr Ala  70 75 80 85 acc gcc tca tcc ggc gtc gtg acc tcc gcg ctc ccg cag tac acc gat 403 Thr Ala Ser Ser Gly Val Val Thr Ser Ala Leu Pro Gln Tyr Thr Asp                  90 95 100 ccg cgc tac ccc ctc ggc aaa gac gac ctg ccc aag gca acc atc gac 451 Pro Arg Tyr Pro Leu Gly Lys Asp Asp Leu Pro Lys Ala Thr Ile Asp             105 110 115 atg gag cca gaa gtt ctt gcg cgc ctt gag cga ttc gtc ggc gtt gac 499 Met Glu Pro Glu Val Leu Ala Arg Leu Glu Arg Phe Val Gly Val Asp         120 125 130 ggt gat cgc atc cgc caa atc aac gcg tac tcg cca tca atg gga cgc 547 Gly Asp Arg Ile Arg Gln Ile Asn Ala Tyr Ser Pro Ser Met Gly Arg     135 140 145 acc att cct cta gtc tgg gtt gtt cca gaa gac aac acc gtg cct ggc 595 Thr Ile Pro Leu Val Trp Val Val Pro Glu Asp Asn Thr Val Pro Gly 150 155 160 165 cca acg gtc tac gca ctc gga ggc ggt gac ggt gga caa ggc ggc cag 643 Pro Thr Val Tyr Ala Leu Gly Gly Gly Asp Gly Gly Gln Gly Gly Gln                 170 175 180 aac tgg gtc acc cgc acc gac ctt gag gaa tta acc agt gac aac aac 691 Asn Trp Val Thr Arg Thr Asp Leu Glu Glu Leu Thr Ser Asp Asn Asn             185 190 195 atc aac ctc atc atg ccg atg ctc gga tct ttt agt ttc tac tct gac 739 Ile Asn Leu Ile Met Pro Met Leu Gly Ser Phe Ser Phe Tyr Ser Asp         200 205 210 tgg gca cgc gaa agc caa tcc atg ggt tgt gcg caa cag tgg gaa aca 787 Trp Ala Arg Glu Ser Gln Ser Met Gly Cys Ala Gln Gln Trp Glu Thr     215 220 225 ttg ctc atg cac gaa ctg cct gag ccg ctt gta gcg gcc 826 Leu Leu Met His Glu Leu Pro Glu Pro Leu Val Ala Ala 230 235 240 <210> 44 <211> 242 <212> PRT <213> Corynebacterium glutamicum <400> 44 Met His Ser Lys Glu Glu Leu Thr Val Arg Lys Gly Ile Ser Arg Val   1 5 10 15 Leu Ser Val Ala Val Ala Ser Ser Ile Gly Phe Gly Thr Val Leu Thr              20 25 30 Gly Thr Gly Ile Ala Ala Ala Gln Asp Ser Ala Phe Asp Tyr Gly Met          35 40 45 Asp Pro Asn Met Asn Tyr Asn Pro Ile Asp Asp Ile Lys Asp Arg Pro      50 55 60 Glu Gly Leu Ser Asn Leu Pro Tyr Phe Gly Ser Lys Leu Thr Ser Trp  65 70 75 80 Gly Ser Ser Tyr Ala Thr Ala Ser Ser Gly Val Val Thr Ser Ala Leu                  85 90 95 Pro Gln Tyr Thr Asp Pro Arg Tyr Pro Leu Gly Lys Asp Asp Leu Pro             100 105 110 Lys Ala Thr Ile Asp Met Glu Pro Glu Val Leu Ala Arg Leu Glu Arg         115 120 125 Phe Val Gly Val Asp Gly Asp Arg Ile Arg Gln Ile Asn Ala Tyr Ser     130 135 140 Pro Ser Met Gly Arg Thr Ile Pro Leu Val Trp Val Val Pro Glu Asp 145 150 155 160 Asn Thr Val Pro Gly Pro Thr Val Tyr Ala Leu Gly Gly Gly Asp Gly                 165 170 175 Gly Gln Gly Gly Gln Asn Trp Val Thr Arg Thr Asp Leu Glu Glu Leu             180 185 190 Thr Ser Asp Asn Asn Ile Asn Leu Ile Met Pro Met Leu Gly Ser Phe         195 200 205 Ser Phe Tyr Ser Asp Trp Ala Arg Glu Ser Gln Ser Met Gly Cys Ala     210 215 220 Gln Gln Trp Glu Thr Leu Leu Met His Glu Leu Pro Glu Pro Leu Val 225 230 235 240 Ala Ala          <210> 45 <211> 653 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (1) .. (630) <223> RXN03039 <220> <221> VARIANT <222> 141 <223> Xaa = Leu <220> <221> VARIANT <222> 142 X223 = Pro <220> <221> VARIANT <222> 166, 180 <223> Xaa = Ser <220> <221> VARIANT <222> 178 <223> Xaa = Tyr <220> <221> VARIANT <222> 195 <223> Xaa = Gly <400> 45 gca ctc ccg caa tac acc gac cca cgc tac ccc ctc ggc aaa gac gac 48 Ala Leu Pro Gln Tyr Thr Asp Pro Arg Tyr Pro Leu Gly Lys Asp Asp   1 5 10 15 ctg ccc aaa gca acc atc gac atg gag cca gaa gct ctt gcg cgc ctt 96 Leu Pro Lys Ala Thr Ile Asp Met Glu Pro Glu Ala Leu Ala Arg Leu              20 25 30 gag cga ttc gtc ggc gtt gac ggt gat cgc atc cgc caa atc aac gcg 144 Glu Arg Phe Val Gly Val Asp Gly Asp Arg Ile Arg Gln Ile Asn Ala          35 40 45 tac tcg cca tca atg gga cgc acc att cct cta gtc tgg gtc gtg cca 192 Tyr Ser Pro Ser Met Gly Arg Thr Ile Pro Leu Val Trp Val Val Pro      50 55 60 gaa gac aac acc gtg cct ggc cca acg gtc tac gca ctc ggc ggc ggc 240 Glu Asp Asn Thr Val Pro Gly Pro Thr Val Tyr Ala Leu Gly Gly Gly  65 70 75 80 gac ggt ggc caa ggc ggc caa aac tgg gtc acc cgc acc gac ctt gat 288 Asp Gly Gly Gln Gly Gly Gln Asn Trp Val Thr Arg Thr Asp Leu Asp                  85 90 95 gag ttg acc agt gaa aac aac atc aac ctc atc atg ccc atg ctc gga 336 Glu Leu Thr Ser Glu Asn Asn Ile Asn Leu Ile Met Pro Met Leu Gly             100 105 110 tct ttt agt ttc tac gct gac tgg gca ggc gaa agc gaa tcc atg ggt 384 Ser Phe Ser Phe Tyr Ala Asp Trp Ala Gly Glu Ser Glu Ser Met Gly         115 120 125 ggt gcg caa cag tgg gaa aca ttc ctc atg cac gaa ctr ccm gag ccg 432 Gly Ala Gln Gln Trp Glu Thr Phe Leu Met His Glu Xaa Xaa Glu Pro     130 135 140 cta gaa gcg gcc atc ggc gca gac ggg caa cgc agc atc gtc ggc atg 480 Leu Glu Ala Ala Ile Gly Ala Asp Gly Gln Arg Ser Ile Val Gly Met 145 150 155 160 tcc atg tcc ggg gga tcr gtg ctg aac ttt gcg acg cat gac ccc aac 528 Ser Met Ser Gly Gly Xaa Val Leu Asn Phe Ala Thr His Asp Pro Asn                 165 170 175 ttt tay tcc tck gtc ggc tca ttt tct gga tgt gcc gaa acc aac tcc 576 Phe Xaa Ser Xaa Val Gly Ser Phe Ser Gly Cys Ala Glu Thr Asn Ser             180 185 190 tgg atg ggr cgc cgn tgg cat cgc agc cac tgc cta caa cgg caa tgt 624 Trp Met Xaa Arg Arg Trp His Arg Ser His Cys Leu Gln Arg Gln Cys         195 200 205 cgt gcc tgagcaaatc tttggtgaag tag 653 Arg ala     210 <210> 46 <211> 210 <212> PRT <213> Corynebacterium glutamicum <220> <221> VARIANT <222> 141 <223> Xaa = Leu <220> <221> VARIANT <222> 142 X223 = Pro <220> <221> VARIANT <222> 166, 180 <223> Xaa = Ser <220> <221> VARIANT <222> 178 <223> Xaa = Tyr <220> <221> VARIANT <222> 195 <223> Xaa = Gly <400> 46 Ala Leu Pro Gln Tyr Thr Asp Pro Arg Tyr Pro Leu Gly Lys Asp Asp   1 5 10 15 Leu Pro Lys Ala Thr Ile Asp Met Glu Pro Glu Ala Leu Ala Arg Leu              20 25 30 Glu Arg Phe Val Gly Val Asp Gly Asp Arg Ile Arg Gln Ile Asn Ala          35 40 45 Tyr Ser Pro Ser Met Gly Arg Thr Ile Pro Leu Val Trp Val Val Pro      50 55 60 Glu Asp Asn Thr Val Pro Gly Pro Thr Val Tyr Ala Leu Gly Gly Gly  65 70 75 80 Asp Gly Gly Gln Gly Gly Gln Asn Trp Val Thr Arg Thr Asp Leu Asp                  85 90 95 Glu Leu Thr Ser Glu Asn Asn Ile Asn Leu Ile Met Pro Met Leu Gly             100 105 110 Ser Phe Ser Phe Tyr Ala Asp Trp Ala Gly Glu Ser Glu Ser Met Gly         115 120 125 Gly Ala Gln Gln Trp Glu Thr Phe Leu Met His Glu Xaa Xaa Glu Pro     130 135 140 Leu Glu Ala Ala Ile Gly Ala Asp Gly Gln Arg Ser Ile Val Gly Met 145 150 155 160 Ser Met Ser Gly Gly Xaa Val Leu Asn Phe Ala Thr His Asp Pro Asn                 165 170 175 Phe Xaa Ser Xaa Val Gly Ser Phe Ser Gly Cys Ala Glu Thr Asn Ser             180 185 190 Trp Met Xaa Arg Arg Trp His Arg Ser His Cys Leu Gln Arg Gln Cys         195 200 205 Arg ala     210 <210> 47 <211> 432 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (409) <223> RXN03040 <220> <221> misc_feature <222> 25, 108, 395 N = a, t, c, or g <220> <221> VARIANT <222> 3 Xaa = Phe, Ser, Tyr, or Cys <220> <221> VARIANT <222> 99 <223> Xaa = Phe, Leu, Ile, or Val <400> 47 attactctcg ctataacgat ccttntgctc aacgctgcga agctcgaaga acaagacaac 60 ctctacatct tcgccggttc cggtgtgttc tctgaactag atg tca tnc ggt gac 115                                             Met Ser Xaa Gly Asp                                               1 5 aac gca ccg att gat gag gat gcg ttc aaa aac cgc gtc ttg gtt ggg 163 Asn Ala Pro Ile Asp Glu Asp Ala Phe Lys Asn Arg Val Leu Val Gly                  10 15 20 ttt gaa atc gaa gct atg tcc aac acc tgc acc cat aac ctc aag gct 211 Phe Glu Ile Glu Ala Met Ser Asn Thr Cys Thr His Asn Leu Lys Ala              25 30 35 gcg acc gat caa atg ggc atc gac aac atc aac tac gat ttc cga cca 259 Ala Thr Asp Gln Met Gly Ile Asp Asn Ile Asn Tyr Asp Phe Arg Pro          40 45 50 acc gga acc cac gcc tgg gat tac tgg aac gaa gcg ctc cac cgc ttc 307 Thr Gly Thr His Ala Trp Asp Tyr Trp Asn Glu Ala Leu His Arg Phe      55 60 65 ttc ccg ttg atg atg cag ggc ttc ggc ctc gac ggt ggt ccc atc ccg 355 Phe Pro Leu Met Met Gln Gly Phe Gly Leu Asp Gly Gly Pro Ile Pro  70 75 80 85 atc tat aac cct aac ggt gtg acc tcc agc gag tct tct ntc aga act 403 Ile Tyr Asn Pro Asn Gly Val Thr Ser Ser Glu Ser Ser Xaa Arg Thr                  90 95 100 gtc ttc tgatgtgagc cttggcaccn gtg 432 Val Phe          <210> 48 <211> 103 <212> PRT <213> Corynebacterium glutamicum <220> <221> VARIANT <222> 3 Xaa = Phe, Ser, Tyr, or Cys <220> <221> VARIANT <222> 99 <223> Xaa = Phe, Leu, Ile, or Val <400> 48 Met Ser Xaa Gly Asp Asn Ala Pro Ile Asp Glu Asp Ala Phe Lys Asn   1 5 10 15 Arg Val Leu Val Gly Phe Glu Ile Glu Ala Met Ser Asn Thr Cys Thr              20 25 30 His Asn Leu Lys Ala Ala Thr Asp Gln Met Gly Ile Asp Asn Ile Asn          35 40 45 Tyr Asp Phe Arg Pro Thr Gly Thr His Ala Trp Asp Tyr Trp Asn Glu      50 55 60 Ala Leu His Arg Phe Phe Pro Leu Met Met Gln Gly Phe Gly Leu Asp  65 70 75 80 Gly Gly Pro Ile Pro Ile Tyr Asn Pro Asn Gly Val Thr Ser Ser Glu                  85 90 95 Ser Ser Xaa Arg Thr Val Phe             100 <210> 49 <211> 835 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (835) <223> RXN03051 <400> 49 acatccagaa gtagtcgttg agtatcacga gcaagtcaac gatagtaaag ataatgtcga 60 ggaactcccg ctgcctaagc gggacatagt tgcaggggac atg cgt tca gat gtt 115                                             Met Arg Ser Asp Val                                               1 5 atc gag tta ccg gag ggg gta agc aag gag aaa gct gac cag cta gaa 163 Ile Glu Leu Pro Glu Gly Val Ser Lys Glu Lys Ala Asp Gln Leu Glu                  10 15 20 gtt gcg gaa gcg cga ctt aac gag ggt gca cga ctg atg gca acc acc 211 Val Ala Glu Ala Arg Leu Asn Glu Gly Ala Arg Leu Met Ala Thr Thr              25 30 35 ggg tgt gag gtt atg tgg cca acg ggc ttc tca gtt tgt ggc cga att 259 Gly Cys Glu Val Met Trp Pro Thr Gly Phe Ser Val Cys Gly Arg Ile          40 45 50 ctt gac acc tat cgc cag gtt gga ggt cag ttg tca tgg ctt ggg cca 307 Leu Asp Thr Tyr Arg Gln Val Gly Gly Gln Leu Ser Trp Leu Gly Pro      55 60 65 ccg aag tca aac gag ttg acc aat ccc gac ggt gtt ggc aaa aga agt 355 Pro Lys Ser Asn Glu Leu Thr Asn Pro Asp Gly Val Gly Lys Arg Ser  70 75 80 85 gaa ttt ttt ggt gga gcc atc tat tgg cac cca gac aca ggc gct tat 403 Glu Phe Phe Gly Gly Ala Ile Tyr Trp His Pro Asp Thr Gly Ala Tyr                  90 95 100 gca gtg acc ttg gac ggt ttg cga cag tgg ggg acc ttg aac tgg gaa 451 Ala Val Thr Leu Asp Gly Leu Arg Gln Trp Gly Thr Leu Asn Trp Glu             105 110 115 tca ggg cca ttg ggg tac cca acc tct ggt ccg atg gat aca aac tat 499 Ser Gly Pro Leu Gly Tyr Pro Thr Ser Gly Pro Met Asp Thr Asn Tyr         120 125 130 ccc ctt act cag cga cag act ttt caa ggt ggt gac aac tac tac aac 547 Pro Leu Thr Gln Arg Gln Thr Phe Gln Gly Gly Asp Asn Tyr Tyr Asn     135 140 145 cca ttg act ggc ggt gct gtg tgg ggc gat att aaa cag cgc tac gaa 595 Pro Leu Thr Gly Gly Ala Val Trp Gly Asp Ile Lys Gln Arg Tyr Glu 150 155 160 165 gaa ctt ggc ggc tcg aat cat gcc att ggc atc ccg atc act aat gag 643 Glu Leu Gly Gly Ser Asn His Ala Ile Gly Ile Pro Ile Thr Asn Glu                 170 175 180 cta cct agc ggt act gag tat ttt tac aat aat ttc tcc aat gga aca 691 Leu Pro Ser Gly Thr Glu Tyr Phe Tyr Asn Asn Phe Ser Asn Gly Thr             185 190 195 att tcg tgg cga aat gat cgt cag aca cgg ttt atg tat ttg gct acg 739 Ile Ser Trp Arg Asn Asp Arg Gln Thr Arg Phe Met Tyr Leu Ala Thr         200 205 210 cag cgg gtg tgg gat gcg ttg ggt cgg gag acg ggt cgt tta ggt ttt 787 Gln Arg Val Trp Asp Ala Leu Gly Arg Glu Thr Gly Arg Leu Gly Phe     215 220 225 cct gaa gca gat gaa aca cct gag gtt tct ggt cta ttc cat gtg gcg 835 Pro Glu Ala Asp Glu Thr Pro Glu Val Ser Gly Leu Phe His Val Ala 230 235 240 245 <210> 50 <211> 245 <212> PRT <213> Corynebacterium glutamicum <400> 50 Met Arg Ser Asp Val Ile Glu Leu Pro Glu Gly Val Ser Lys Glu Lys   1 5 10 15 Ala Asp Gln Leu Glu Val Ala Glu Ala Arg Leu Asn Glu Gly Ala Arg              20 25 30 Leu Met Ala Thr Thr Gly Cys Glu Val Met Trp Pro Thr Gly Phe Ser          35 40 45 Val Cys Gly Arg Ile Leu Asp Thr Tyr Arg Gln Val Gly Gly Gln Leu      50 55 60 Ser Trp Leu Gly Pro Pro Lys Ser Asn Glu Leu Thr Asn Pro Asp Gly  65 70 75 80 Val Gly Lys Arg Ser Glu Phe Phe Gly Gly Ala Ile Tyr Trp His Pro                  85 90 95 Asp Thr Gly Ala Tyr Ala Val Thr Leu Asp Gly Leu Arg Gln Trp Gly             100 105 110 Thr Leu Asn Trp Glu Ser Gly Pro Leu Gly Tyr Pro Thr Ser Gly Pro         115 120 125 Met Asp Thr Asn Tyr Pro Leu Thr Gln Arg Gln Thr Phe Gln Gly Gly     130 135 140 Asp Asn Tyr Tyr Asn Pro Leu Thr Gly Gly Ala Val Trp Gly Asp Ile 145 150 155 160 Lys Gln Arg Tyr Glu Glu Leu Gly Gly Ser Asn His Ala Ile Gly Ile                 165 170 175 Pro Ile Thr Asn Glu Leu Pro Ser Gly Thr Glu Tyr Phe Tyr Asn Asn             180 185 190 Phe Ser Asn Gly Thr Ile Ser Trp Arg Asn Asp Arg Gln Thr Arg Phe         195 200 205 Met Tyr Leu Ala Thr Gln Arg Val Trp Asp Ala Leu Gly Arg Glu Thr     210 215 220 Gly Arg Leu Gly Phe Pro Glu Ala Asp Glu Thr Pro Glu Val Ser Gly 225 230 235 240 Leu Phe His Val Ala                 245 <210> 51 <211> 1704 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (1681) <223> RXN03054 <400> 51 ggtggaaata cgcgcacaac aattttattc acagaactta tgattttttc gggttagggt 60 cagtttgttc acatcaacta gtaacgaaag gatcatgtga atg aaa ctg ttt tcc 115                                             Met Lys Leu Phe Ser                                               1 5 aag gct gca ggc gtc att gct gca gca ctt ctt gtt gca ggt ggt ata 163 Lys Ala Ala Gly Val Ile Ala Ala Ala Leu Leu Val Ala Gly Gly Ile                  10 15 20 gca cct gtg gca cag ggg caa gct agt cag gtg gtc aca cct gaa gac 211 Ala Pro Val Ala Gln Gly Gln Ala Ser Gln Val Val Thr Pro Glu Asp              25 30 35 caa gat gcg tat gtt caa cag ttc cac cac gaa ggg aat acc cca cct 259 Gln Asp Ala Tyr Val Gln Gln Phe His His Glu Gly Asn Thr Pro Pro          40 45 50 gtg gta gac ggg gtg ggt ggc tac act gag caa gaa atc gcc gag atc 307 Val Val Asp Gly Val Gly Gly Tyr Thr Glu Gln Glu Ile Ala Glu Ile      55 60 65 cac gag gct atc cga caa gcc caa gaa tct ggc gca cct aat gaa gag 355 His Glu Ala Ile Arg Gln Ala Gln Glu Ser Gly Ala Pro Asn Glu Glu  70 75 80 85 ctc att ccg ggt gag atg tgg tca gat aag gtg gag ctg cca gta act 403 Leu Ile Pro Gly Glu Met Trp Ser Asp Lys Val Glu Leu Pro Val Thr                  90 95 100 att gat aaa gca gcc gct gat gag gca gag ata gct att gca cag caa 451 Ile Asp Lys Ala Ala Ala Asp Glu Ala Glu Ile Ala Ile Ala Gln Gln             105 110 115 caa tct cag cca cag acg cga ggc ctt gct gcg gct gcg gcg tgt cag 499 Gln Ser Gln Pro Gln Thr Arg Gly Leu Ala Ala Ala Ala Ala Cys Gln         120 125 130 acg ttt tgg ccg tca cct cat cag gtt tgt ggt gct att tta gag cgc 547 Thr Phe Trp Pro Ser Pro His Gln Val Cys Gly Ala Ile Leu Glu Arg     135 140 145 tat att cag cag ggt gcc cag ttt ggg tgg atg ttg ttt ccg agt gaa 595 Tyr Ile Gln Gln Gly Ala Gln Phe Gly Trp Met Leu Phe Pro Ser Glu 150 155 160 165 ggc caa acg tta aat cct gat ggt cag ggg tat cgt cag cgg ttt atg 643 Gly Gln Thr Leu Asn Pro Asp Gly Gln Gly Tyr Arg Gln Arg Phe Met                 170 175 180 aat ggg ttt gtt tat tgg cat ccg aca act ggt gcg cat gct gtt aat 691 Asn Gly Phe Val Tyr Trp His Pro Thr Thr Gly Ala His Ala Val Asn             185 190 195 aat tac agt gcg cag gtg tgg gag cgt aat ggg tgg gag tct ggg tgg 739 Asn Tyr Ser Ala Gln Val Trp Glu Arg Asn Gly Trp Glu Ser Gly Trp         200 205 210 atg ggt tat ccc act ggt ggt gaa gtc cct gtg aat ggt tcc aat ccg 787 Met Gly Tyr Pro Thr Gly Gly Glu Val Pro Val Asn Gly Ser Asn Pro     215 220 225 att gat ggt gag ttg agt ggg tgg gtg caa act ttc caa ggt ggg cga 835 Ile Asp Gly Glu Leu Ser Gly Trp Val Gln Thr Phe Gln Gly Gly Arg 230 235 240 245 gtg tat cgc agt ccg gta ttg gac ggt ttc cag gtg gcc agt att aat 883 Val Tyr Arg Ser Pro Val Leu Asp Gly Phe Gln Val Ala Ser Ile Asn                 250 255 260 ggg ctg atc ttg gat aaa tgg ctt gaa ttg ggt ggt cct gat agt gac 931 Gly Leu Ile Leu Asp Lys Trp Leu Glu Leu Gly Gly Pro Asp Ser Asp             265 270 275 ctt ggt ttt ccc att gcg gat gag gct gtg aca gct gac ggt gtg ggt 979 Leu Gly Phe Pro Ile Ala Asp Glu Ala Val Thr Ala Asp Gly Val Gly         280 285 290 aga ttt tct gtt ttc cag aac gga gtt gtc tac tgg cat ccg caa cac 1027 Arg Phe Ser Val Phe Gln Asn Gly Val Val Tyr Trp His Pro Gln His     295 300 305 gga gct cac cct ata tta ggg aat ata tac agt atc tgg aga gaa gaa 1075 Gly Ala His Pro Ile Leu Gly Asn Ile Tyr Ser Ile Trp Arg Glu Glu 310 315 320 325 gga gct gag agt ggg gaa ttc ggt tac cct atc ggc gat cca gaa aag 1123 Gly Ala Glu Ser Gly Glu Phe Gly Tyr Pro Ile Gly Asp Pro Glu Lys                 330 335 340 tat aca gaa aac atg gct aat cag gta ttc gaa aaa ggc gaa ctt gca 1171 Tyr Thr Glu Asn Met Ala Asn Gln Val Phe Glu Lys Gly Glu Leu Ala             345 350 355 gct aac cta tac ccc aat cct ctt gag gct ttt att gag ttt tta ccc 1219 Ala Asn Leu Tyr Pro Asn Pro Leu Glu Ala Phe Ile Glu Phe Leu Pro         360 365 370 ttt gct aat ctt gag gaa gca ata gag tat ttt gag aac gga ttg tca 1267 Phe Ala Asn Leu Glu Glu Ala Ile Glu Tyr Phe Glu Asn Gly Leu Ser     375 380 385 aat tct cgt gta gag gcg aat tca ctt aac gcc aag aaa gat tcg att 1315 Asn Ser Arg Val Glu Ala Asn Ser Leu Asn Ala Lys Lys Asp Ser Ile 390 395 400 405 caa tgt caa tcg caa tcc gct aac att cat gtg aga acg aag agt gac 1363 Gln Cys Gln Ser Gln Ser Ala Asn Ile His Val Arg Thr Lys Ser Asp                 410 415 420 gga gtc ggg att agg gtt cca aag att ggg ttt aag gct agg atg gat 1411 Gly Val Gly Ile Arg Val Pro Lys Ile Gly Phe Lys Ala Arg Met Asp             425 430 435 tgc gac ctt cct gga act gtc tca gat gta gtg ggg tat gga tgg att 1459 Cys Asp Leu Pro Gly Thr Val Ser Asp Val Val Gly Tyr Gly Trp Ile         440 445 450 tac tac gac tat tgg gga cga tgg gct caa gca gca tat gca caa caa 1507 Tyr Tyr Asp Tyr Trp Gly Arg Trp Ala Gln Ala Ala Tyr Ala Gln Gln     455 460 465 ttc ttc ggt aat agg aat tct gtt gtg caa acc aat tta gag gcg ggt 1555 Phe Phe Gly Asn Arg Asn Ser Val Val Gln Thr Asn Leu Glu Ala Gly 470 475 480 485 tgc agc ggg gag aag aat aca tta ttt tgg ggt act tca tat ttt cag 1603 Cys Ser Gly Glu Lys Asn Thr Leu Phe Trp Gly Thr Ser Tyr Phe Gln                 490 495 500 gtg act tat gaa ggt cag ccg tat ttc ggt cag tca gca act aat tac 1651 Val Thr Tyr Glu Gly Gln Pro Tyr Phe Gly Gln Ser Ala Thr Asn Tyr             505 510 515 gct tat ctt ccg tgt acg ata gac cgt agt taacataagg aatggaatag 1701 Ala Tyr Leu Pro Cys Thr Ile Asp Arg Ser         520 525 gag 1704 <210> 52 <211> 527 <212> PRT <213> Corynebacterium glutamicum <400> 52 Met Lys Leu Phe Ser Lys Ala Ala Gly Val Ile Ala Ala Ala Leu Leu   1 5 10 15 Val Ala Gly Gly Ile Ala Pro Val Ala Gln Gly Gln Ala Ser Gln Val              20 25 30 Val Thr Pro Glu Asp Gln Asp Ala Tyr Val Gln Gln Phe His His Glu          35 40 45 Gly Asn Thr Pro Pro Val Val Asp Gly Val Gly Gly Tyr Thr Glu Gln      50 55 60 Glu Ile Ala Glu Ile His Glu Ala Ile Arg Gln Ala Gln Glu Ser Gly  65 70 75 80 Ala Pro Asn Glu Glu Leu Ile Pro Gly Glu Met Trp Ser Asp Lys Val                  85 90 95 Glu Leu Pro Val Thr Ile Asp Lys Ala Ala Ala Asp Glu Ala Glu Ile             100 105 110 Ala Ile Ala Gln Gln Gln Ser Gln Pro Gln Thr Arg Gly Leu Ala Ala         115 120 125 Ala Ala Ala Cys Gln Thr Phe Trp Pro Ser Pro His Gln Val Cys Gly     130 135 140 Ala Ile Leu Glu Arg Tyr Ile Gln Gln Gly Ala Gln Phe Gly Trp Met 145 150 155 160 Leu Phe Pro Ser Glu Gly Gln Thr Leu Asn Pro Asp Gly Gln Gly Tyr                 165 170 175 Arg Gln Arg Phe Met Asn Gly Phe Val Tyr Trp His Pro Thr Thr Gly             180 185 190 Ala His Ala Val Asn Asn Tyr Ser Ala Gln Val Trp Glu Arg Asn Gly         195 200 205 Trp Glu Ser Gly Trp Met Gly Tyr Pro Thr Gly Gly Glu Val Pro Val     210 215 220 Asn Gly Ser Asn Pro Ile Asp Gly Glu Leu Ser Gly Trp Val Gln Thr 225 230 235 240 Phe Gln Gly Gly Arg Val Tyr Arg Ser Pro Val Leu Asp Gly Phe Gln                 245 250 255 Val Ala Ser Ile Asn Gly Leu Ile Leu Asp Lys Trp Leu Glu Leu Gly             260 265 270 Gly Pro Asp Ser Asp Leu Gly Phe Pro Ile Ala Asp Glu Ala Val Thr         275 280 285 Ala Asp Gly Val Gly Arg Phe Ser Val Phe Gln Asn Gly Val Val Tyr     290 295 300 Trp His Pro Gln His Gly Ala His Pro Ile Leu Gly Asn Ile Tyr Ser 305 310 315 320 Ile Trp Arg Glu Glu Gly Ala Glu Ser Gly Glu Phe Gly Tyr Pro Ile                 325 330 335 Gly Asp Pro Glu Lys Tyr Thr Glu Asn Met Ala Asn Gln Val Phe Glu             340 345 350 Lys Gly Glu Leu Ala Ala Asn Leu Tyr Pro Asn Pro Leu Glu Ala Phe         355 360 365 Ile Glu Phe Leu Pro Phe Ala Asn Leu Glu Glu Ala Ile Glu Tyr Phe     370 375 380 Glu Asn Gly Leu Ser Asn Ser Arg Val Glu Ala Asn Ser Leu Asn Ala 385 390 395 400 Lys Lys Asp Ser Ile Gln Cys Gln Ser Gln Ser Ala Asn Ile His Val                 405 410 415 Arg Thr Lys Ser Asp Gly Val Gly Ile Arg Val Pro Lys Ile Gly Phe             420 425 430 Lys Ala Arg Met Asp Cys Asp Leu Pro Gly Thr Val Ser Asp Val Val         435 440 445 Gly Tyr Gly Trp Ile Tyr Tyr Asp Tyr Trp Gly Arg Trp Ala Gln Ala     450 455 460 Ala Tyr Ala Gln Gln Phe Phe Gly Asn Arg Asn Ser Val Val Gln Thr 465 470 475 480 Asn Leu Glu Ala Gly Cys Ser Gly Glu Lys Asn Thr Leu Phe Trp Gly                 485 490 495 Thr Ser Tyr Phe Gln Val Thr Tyr Glu Gly Gln Pro Tyr Phe Gly Gln             500 505 510 Ser Ala Thr Asn Tyr Ala Tyr Leu Pro Cys Thr Ile Asp Arg Ser         515 520 525 <210> 53 <211> 456 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <101> (101) .. (433) <223> RXN02949 <400> 53 actctcgaag gttgaacaca gggctgcgat tgtgctggat caaatgtctg cacgaaaaat 60 tgttatcgcc cctggatgag tagtgattta gaggagtgct gtg agc gac gag cag 115                                             Val Ser Asp Glu Gln                                               1 5 aat tct ggc gta ggc gga acg tct cgc cca acg ggt aaa cgc cag ctg 163 Asn Ser Gly Val Gly Gly Thr Ser Arg Pro Thr Gly Lys Arg Gln Leu                  10 15 20 tcg ggt gct tcc act acc tct acc tct tct tat gag gct aag cag gta 211 Ser Gly Ala Ser Thr Thr Ser Ser Thr Ser Ser Tyr Glu Ala Lys Gln Val              25 30 35 tct aca cag aag aag tca tcc ggt tcg gat tct aag cct ggc ggc ggt 259 Ser Thr Gln Lys Lys Ser Ser Gly Ser Asp Ser Lys Pro Gly Gly Gly          40 45 50 gtt att tct ttt ctg cct gag gtt gtg gga gaa gtc cgt aag gtt att 307 Val Ile Ser Phe Leu Pro Glu Val Val Gly Glu Val Arg Lys Val Ile      55 60 65 tgg cct act gcg cgc cag atg gtc acg tac acc ctt gtc gtt ttg gga 355 Trp Pro Thr Ala Arg Gln Met Val Thr Tyr Thr Leu Val Val Leu Gly  70 75 80 85 ttc ttg att gtt ttg acc gct ttg gtg tct ggt gtg gat ttc cta gct 403 Phe Leu Ile Val Leu Thr Ala Leu Val Ser Gly Val Asp Phe Leu Ala                  90 95 100 ggt ctt gga gtt gag aag att ctg act ccg taggtaggat gtgtaacatc 453 Gly Leu Gly Val Glu Lys Ile Leu Thr Pro             105 110 ttt 456 <210> 54 <211> 111 <212> PRT <213> Corynebacterium glutamicum <400> 54 Val Ser Asp Glu Gln Asn Ser Gly Val Gly Gly Thr Ser Arg Pro Thr   1 5 10 15 Gly Lys Arg Gln Leu Ser Gly Ala Ser Thr Thr Ser Thr Ser Ser Tyr              20 25 30 Glu Ala Lys Gln Val Ser Thr Gln Lys Lys Ser Ser Gly Ser Asp Ser          35 40 45 Lys Pro Gly Gly Gly Val Ile Ser Phe Leu Pro Glu Val Val Gly Glu      50 55 60 Val Arg Lys Val Ile Trp Pro Thr Ala Arg Gln Met Val Thr Tyr Thr  65 70 75 80 Leu Val Val Leu Gly Phe Leu Ile Val Leu Thr Ala Leu Val Ser Gly                  85 90 95 Val Asp Phe Leu Ala Gly Leu Gly Val Glu Lys Ile Leu Thr Pro             100 105 110 <210> 55 <211> 1941 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1918) <223> RXN02462 <400> 55 tccatcctca tcgacgaagc ccgcacccca ctgattatct ccgggaccag tagacggcac 60 atcgcagttc tacaacgtct tcgcacagat cgtcccacgc atg acc aag gac gtt 115                                             Met Thr Lys Asp Val                                               1 5 cac tac gaa gtc gac gaa cgt aaa aag acc gtc ggt gtg aaa gaa gaa 163 His Tyr Glu Val Asp Glu Arg Lys Lys Thr Val Gly Val Lys Glu Glu                  10 15 20 ggc gtc gaa tac gtc gaa gac caa ctc ggc atc gac aac ctc tac gca 211 Gly Val Glu Tyr Val Glu Asp Gln Leu Gly Ile Asp Asn Leu Tyr Ala              25 30 35 cct gag cac tca cag ctg gtc agc tac ctg aac aac gcc atc aag gca 259 Pro Glu His Ser Gln Leu Val Ser Tyr Leu Asn Asn Ala Ile Lys Ala          40 45 50 cag gaa ctg ttc acc cgc gac aag gac tac atc gtc cgc aac ggc gaa 307 Gln Glu Leu Phe Thr Arg Asp Lys Asp Tyr Ile Val Arg Asn Gly Glu      55 60 65 gtt atg atc gtc gac ggc ttc acc ggc cgt gtc ctt gcc ggc cgc cga 355 Val Met Ile Val Asp Gly Phe Thr Gly Arg Val Leu Ala Gly Arg Arg  70 75 80 85 tac aac gaa ggc atg cac cag gcg atc gaa gcc aaa gag cgc gta gag 403 Tyr Asn Glu Gly Met His Gln Ala Ile Glu Ala Lys Glu Arg Val Glu                  90 95 100 atc aaa aac gag aac cag acc ctg gcg acc gtt acc ctc cag aac tac 451 Ile Lys Asn Glu Asn Gln Thr Leu Ala Thr Val Thr Leu Gln Asn Tyr             105 110 115 ttc cgc ctc tac acc aaa ctc gcc ggc atg acc ggt acc gca gag acc 499 Phe Arg Leu Tyr Thr Lys Leu Ala Gly Met Thr Gly Thr Ala Glu Thr         120 125 130 gaa gca gca gag ctc aac cag atc tac aag ctc gac gtc atc gcg atc 547 Glu Ala Ala Glu Leu Asn Gln Ile Tyr Lys Leu Asp Val Ile Ala Ile     135 140 145 cca acc aac cga cca aac cag cgc gaa gac ttg acc gac ttg gtg tac 595 Pro Thr Asn Arg Pro Asn Gln Arg Glu Asp Leu Thr Asp Leu Val Tyr 150 155 160 165 aaa acc caa gag gct aag ttc gca gca gtc gtc gac gac atc gca gaa 643 Lys Thr Gln Glu Ala Lys Phe Ala Ala Val Val Asp Asp Ile Ala Glu                 170 175 180 cgc acc gaa aag ggc caa cca gtc ctc gtc ggt acc gtc tcc gtc gag 691 Arg Thr Glu Lys Gly Gln Pro Val Leu Val Gly Thr Val Ser Val Glu             185 190 195 cgc tcc gaa tac ctc tcc cag ctg ttg acc aaa cga ggc atc aag cac 739 Arg Ser Glu Tyr Leu Ser Gln Leu Leu Thr Lys Arg Gly Ile Lys His         200 205 210 aac gtc ctc aat gcg aag cac cac gag cag gaa gca cag atc gtt gct 787 Asn Val Leu Asn Ala Lys His His Glu Gln Glu Ala Gln Ile Val Ala     215 220 225 cag gca ggt ctt cca ggc gcc gtc acc gtt gcc acc aac atg gcg ggc 835 Gln Ala Gly Leu Pro Gly Ala Val Thr Val Ala Thr Asn Met Ala Gly 230 235 240 245 cgt gga acc gac atc gtg ctc ggc gga aac cca gaa atc ctc ctc gac 883 Arg Gly Thr Asp Ile Val Leu Gly Gly Asn Pro Glu Ile Leu Leu Asp                 250 255 260 atc aaa ctc cgc gaa cgt gga ctt gat cct ttc gaa gac gaa gaa agc 931 Ile Lys Leu Arg Glu Arg Gly Leu Asp Pro Phe Glu Asp Glu Glu Ser             265 270 275 tac cag gaa gcc tgg gac gct gaa ctt cca gca atg aag cag cga tgc 979 Tyr Gln Glu Ala Trp Asp Ala Glu Leu Pro Ala Met Lys Gln Arg Cys         280 285 290 gaa gaa cgt ggc gac aaa gtc cgc gaa gcc gga gga ctc tac gtc ctt 1027 Glu Glu Arg Gly Asp Lys Val Arg Glu Ala Gly Gly Leu Tyr Val Leu     295 300 305 ggc acc gaa cgc cac gaa tcc cga cgc atc gac aac cag ctg cgc ggt 1075 Gly Thr Glu Arg His Glu Ser Arg Arg Ile Asp Asn Gln Leu Arg Gly 310 315 320 325 cgt tct gca cgt cag ggc gac cca gga tcc acc cgc ttc tat ctc tct 1123 Arg Ser Ala Arg Gln Gly Asp Pro Gly Ser Thr Arg Phe Tyr Leu Ser                 330 335 340 atg cgc gac gac ctg atg gtt cgc ttc gtc ggc cca acc atg gaa aac 1171 Met Arg Asp Asp Leu Met Val Arg Phe Val Gly Pro Thr Met Glu Asn             345 350 355 atg atg aac agg atc ctc aac gtc cca gac gat gtg ccc atc gaa tcc aaa 1219 Met Met Asn Arg Leu Asn Val Pro Asp Asp Val Pro Ile Glu Ser Lys         360 365 370 acc gtc acc aac tcc atc aag ggc gcc caa gct cag gtg gag aac cag 1267 Thr Val Thr Asn Ser Ile Lys Gly Ala Gln Ala Gln Val Glu Asn Gln     375 380 385 aac ttc gaa atg cgt aag aac gtt ctg aag tac gac gaa gtc atg aac 1315 Asn Phe Glu Met Arg Lys Asn Val Leu Lys Tyr Asp Glu Val Met Asn 390 395 400 405 gaa cag cgc aag gtt atc tac agc gag cga cgc gaa atc ctc gaa tcc 1363 Glu Gln Arg Lys Val Ile Tyr Ser Glu Arg Arg Glu Ile Leu Glu Ser                 410 415 420 gca gac atc tcc cgc tac atc caa aac atg atc gaa gaa aca gtc agc 1411 Ala Asp Ile Ser Arg Tyr Ile Gln Asn Met Ile Glu Glu Thr Val Ser             425 430 435 gca tac gtc gac ggc gcc acc gcc aac ggc tac gtc gaa gac tgg gac 1459 Ala Tyr Val Asp Gly Ala Thr Ala Asn Gly Tyr Val Glu Asp Trp Asp         440 445 450 ctc gac aaa ctc tgg aac gcc ctc gaa gcc ctc tac gac cca tcg atc 1507 Leu Asp Lys Leu Trp Asn Ala Leu Glu Ala Leu Tyr Asp Pro Ser Ile     455 460 465 aac tgg acc gac ctc gtc gaa ggc agc gaa tac ggc aaa cca ggg gag 1555 Asn Trp Thr Asp Leu Val Glu Gly Ser Glu Tyr Gly Lys Pro Gly Glu 470 475 480 485 ctg tcc gcc gaa gat cta cgc acc gca ctc gtc aac gac gcc cac gcc 1603 Leu Ser Ala Glu Asp Leu Arg Thr Ala Leu Val Asn Asp Ala His Ala                 490 495 500 gaa tac gca aaa ctc gaa gaa gcc gta tcc gca atc ggc ggc gaa gca 1651 Glu Tyr Ala Lys Leu Glu Glu Ala Val Ser Ala Ile Gly Gly Glu Ala             505 510 515 cag atc cgc aac atc gaa cga atg gtg ctc atg cca gtc atc gac acc 1699 Gln Ile Arg Asn Ile Glu Arg Met Val Leu Met Pro Val Ile Asp Thr         520 525 530 aaa tgg cgc gaa cac ctc tac gaa atg gac tac ctg aaa gaa ggc atc 1747 Lys Trp Arg Glu His Leu Tyr Glu Met Asp Tyr Leu Lys Glu Gly Ile     535 540 545 ggc ctg cgc gca atg gca cag cgc gac cca ctg gtc gaa tac caa aag 1795 Gly Leu Arg Ala Met Ala Gln Arg Asp Pro Leu Val Glu Tyr Gln Lys 550 555 560 565 gaa ggc ggc gac atg ttc aac ggc atg aaa gac ggc atc aag gaa gaa 1843 Glu Gly Gly Asp Met Phe Asn Gly Met Lys Asp Gly Ile Lys Glu Glu                 570 575 580 acc gtc cgc cag ctc ttc ctc tcc gca agc agt tca tca agc aag acg 1891 Thr Val Arg Gln Leu Phe Leu Ser Ala Ser Ser Ser Ser Ser Lys Thr             585 590 595 cgg aag tcg ctg act aac tca gaa ccc tgaaattcag catccgccac 1938 Arg Lys Ser Leu Thr Asn Ser Glu Pro         600 605 atg 1941 <210> 56 <211> 606 <212> PRT <213> Corynebacterium glutamicum <400> 56 Met Thr Lys Asp Val His Tyr Glu Val Asp Glu Arg Lys Lys Thr Val   1 5 10 15 Gly Val Lys Glu Glu Gly Val Glu Tyr Val Glu Asp Gln Leu Gly Ile              20 25 30 Asp Asn Leu Tyr Ala Pro Glu His Ser Gln Leu Val Ser Tyr Leu Asn          35 40 45 Asn Ala Ile Lys Ala Gln Glu Leu Phe Thr Arg Asp Lys Asp Tyr Ile      50 55 60 Val Arg Asn Gly Glu Val Met Ile Val Asp Gly Phe Thr Gly Arg Val  65 70 75 80 Leu Ala Gly Arg Arg Tyr Asn Glu Gly Met His Gln Ala Ile Glu Ala                  85 90 95 Lys Glu Arg Val Glu Ile Lys Asn Glu Asn Gln Thr Leu Ala Thr Val             100 105 110 Thr Leu Gln Asn Tyr Phe Arg Leu Tyr Thr Lys Leu Ala Gly Met Thr         115 120 125 Gly Thr Ala Glu Thr Glu Ala Ala Glu Leu Asn Gln Ile Tyr Lys Leu     130 135 140 Asp Val Ile Ala Ile Pro Thr Asn Arg Pro Asn Gln Arg Glu Asp Leu 145 150 155 160 Thr Asp Leu Val Tyr Lys Thr Gln Glu Ala Lys Phe Ala Ala Val Val                 165 170 175 Asp Asp Ile Ala Glu Arg Thr Glu Lys Gly Gln Pro Val Leu Val Gly             180 185 190 Thr Val Ser Val Glu Arg Ser Glu Tyr Leu Ser Gln Leu Leu Thr Lys         195 200 205 Arg Gly Ile Lys His Asn Val Leu Asn Ala Lys His His Glu Gln Glu     210 215 220 Ala Gln Ile Val Ala Gln Ala Gly Leu Pro Gly Ala Val Thr Val Ala 225 230 235 240 Thr Asn Met Ala Gly Arg Gly Thr Asp Ile Val Leu Gly Gly Asn Pro                 245 250 255 Glu Ile Leu Leu Asp Ile Lys Leu Arg Glu Arg Gly Leu Asp Pro Phe             260 265 270 Glu Asp Glu Glu Ser Tyr Gln Glu Ala Trp Asp Ala Glu Leu Pro Ala         275 280 285 Met Lys Gln Arg Cys Glu Glu Arg Gly Asp Lys Val Arg Glu Ala Gly     290 295 300 Gly Leu Tyr Val Leu Gly Thr Glu Arg His Glu Ser Arg Arg Ile Asp 305 310 315 320 Asn Gln Leu Arg Gly Arg Ser Ala Arg Gln Gly Asp Pro Gly Ser Thr                 325 330 335 Arg Phe Tyr Leu Ser Met Arg Asp Asp Leu Met Val Arg Phe Val Gly             340 345 350 Pro Thr Met Glu Asn Met Met Asn Arg Leu Asn Val Pro Asp Asp Val         355 360 365 Pro Ile Glu Ser Lys Thr Val Thr Asn Ser Ile Lys Gly Ala Gln Ala     370 375 380 Gln Val Glu Asn Gln Asn Phe Glu Met Arg Lys Asn Val Leu Lys Tyr 385 390 395 400 Asp Glu Val Met Asn Glu Gln Arg Lys Val Ile Tyr Ser Glu Arg Arg                 405 410 415 Glu Ile Leu Glu Ser Ala Asp Ile Ser Arg Tyr Ile Gln Asn Met Ile             420 425 430 Glu Glu Thr Val Ser Ala Tyr Val Asp Gly Ala Thr Ala Asn Gly Tyr         435 440 445 Val Glu Asp Trp Asp Leu Asp Lys Leu Trp Asn Ala Leu Glu Ala Leu     450 455 460 Tyr Asp Pro Ser Ile Asn Trp Thr Asp Leu Val Glu Gly Ser Glu Tyr 465 470 475 480 Gly Lys Pro Gly Glu Leu Ser Ala Glu Asp Leu Arg Thr Ala Leu Val                 485 490 495 Asn Asp Ala His Ala Glu Tyr Ala Lys Leu Glu Glu Ala Val Ser Ala             500 505 510 Ile Gly Gly Glu Ala Gln Ile Arg Asn Ile Glu Arg Met Val Leu Met         515 520 525 Pro Val Ile Asp Thr Lys Trp Arg Glu His Leu Tyr Glu Met Asp Tyr     530 535 540 Leu Lys Glu Gly Ile Gly Leu Arg Ala Met Ala Gln Arg Asp Pro Leu 545 550 555 560 Val Glu Tyr Gln Lys Glu Gly Gly Asp Met Phe Asn Gly Met Lys Asp                 565 570 575 Gly Ile Lys Glu Glu Thr Val Arg Gln Leu Phe Leu Ser Ala Ser Ser             580 585 590 Ser Ser Ser Lys Thr Arg Lys Ser Leu Thr Asn Ser Glu Pro         595 600 605 <210> 57 <211> 1965 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1942) <223> RXN01559 <400> 57 gtctggttga ttggaattga aggagacttt cttggctcgg caaaaaaaga gtgccgctag 60 cgcctgggaa cgatggccaa aacgcgcaat agcgttgttt gtg ctc atc gtc gtt 115                                             Val Leu Ile Val Val                                               1 5 ggt gtt tat gcg ttg gtg ctg ttg aca ggc gat cgt tct gcc aca cca 163 Gly Val Tyr Ala Leu Val Leu Leu Thr Gly Asp Arg Ser Ala Thr Pro                  10 15 20 aaa ttg ggt att gat ctg caa ggc gga acc cga gtg acc ctc gtg ccg 211 Lys Leu Gly Ile Asp Leu Gln Gly Gly Thr Arg Val Thr Leu Val Pro              25 30 35 cag ggg cag gat cca act cag gac cag ctg aat cag gca cgc acc att 259 Gln Gly Gln Asp Pro Thr Gln Asp Gln Leu Asn Gln Ala Arg Thr Ile          40 45 50 ctg gaa aac cgt gtg aac ggc atg ggc gtt tca ggt gca agc gtg gtc 307 Leu Glu Asn Arg Val Asn Gly Met Gly Val Ser Gly Ala Ser Val Val      55 60 65 gct gac ggt aac acg ctg gtg atc act gtt ccc ggg gaa aat acc gca 355 Ala Asp Gly Asn Thr Leu Val Ile Thr Val Pro Gly Glu Asn Thr Ala  70 75 80 85 cag gcg caa tcc cta gga cag acc tcc cag ctg ctg ttc cgt ccc gtt 403 Gln Ala Gln Ser Leu Gly Gln Thr Ser Gln Leu Leu Phe Arg Pro Val                  90 95 100 ggt cag gca gga atg ccc gat atg acc acg ttg atg cca gag ctg gaa 451 Gly Gln Ala Gly Met Pro Asp Met Thr Thr Leu Met Pro Glu Leu Glu             105 110 115 gag atg gcc aac agg tgg gtt gaa tac ggc gtc atc acc gaa gag cag 499 Glu Met Ala Asn Arg Trp Val Glu Tyr Gly Val Ile Thr Glu Glu Gln         120 125 130 gca aat gcc tcc ttg gag gaa atg aac acc gct gtt gca tcg acc act 547 Ala Asn Ala Ser Leu Glu Glu Met Asn Thr Ala Val Ala Ser Thr Thr     135 140 145 gcg gtg gaa ggc gaa gaa gca act gag cca gaa ccc gtc acc gtg tcg 595 Ala Val Glu Gly Glu Glu Ala Thr Glu Pro Glu Pro Val Thr Val Ser 150 155 160 165 gcg acc cct atg gat gag cca gcc aac tcc att gag gca aca cag cga 643 Ala Thr Pro Met Asp Glu Pro Ala Asn Ser Ile Glu Ala Thr Gln Arg                 170 175 180 cgc cag gaa atc acg gac atg ctg cgc acc gac cgc cag tcc acc gat 691 Arg Gln Glu Ile Thr Asp Met Leu Arg Thr Asp Arg Gln Ser Thr Asp             185 190 195 ccc act gtc cag atc gct gca agt tct ttg atg cag tgc acc act gat 739 Pro Thr Val Gln Ile Ala Ala Ser Ser Leu Met Gln Cys Thr Thr Asp         200 205 210 gag atg gat cct ttg gcc ggc acc gat gat cca cgc ctg cca ttg gtg 787 Glu Met Asp Pro Leu Ala Gly Thr Asp Asp Pro Arg Leu Pro Leu Val     215 220 225 gca tgt gat cca gct gta ggt ggc gtg tat gta ctt gat cct gca cct 835 Ala Cys Asp Pro Ala Val Gly Gly Val Tyr Val Leu Asp Pro Ala Pro 230 235 240 245 ttg ctc aac ggc gaa acc gat gag gaa aat ggt gcg cgc cta acc ggt 883 Leu Leu Asn Gly Glu Thr Asp Glu Glu Asn Gly Ala Arg Leu Thr Gly                 250 255 260 aat gag atc gat acc aac cgt ccc atc acc ggt gga ttc aac gcc cag 931 Asn Glu Ile Asp Thr Asn Arg Pro Ile Thr Gly Gly Phe Asn Ala Gln             265 270 275 tcc ggc cag atg gaa atc agc ttt gcc ttc aaa tcc ggc gat ggg gaa 979 Ser Gly Gln Met Glu Ile Ser Phe Ala Phe Lys Ser Gly Asp Gly Glu         280 285 290 gaa ggc tct gca act tgg tcc tct ctg acc agc cag tac ctg cag cag 1027 Glu Gly Ser Ala Thr Trp Ser Ser Leu Thr Ser Gln Tyr Leu Gln Gln     295 300 305 cag atc gcc atc acc ctg gac tct cag gtg att tct gca ccc gtg att 1075 Gln Ile Ala Ile Thr Leu Asp Ser Gln Val Ile Ser Ala Pro Val Ile 310 315 320 325 cag tca gca acc cct gtg ggt tct gca aca tcc atc acc ggt gac ttc 1123 Gln Ser Ala Thr Pro Val Gly Ser Ala Thr Ser Ile Thr Gly Asp Phe                 330 335 340 act caa act gaa gcc caa gat ctg gcg aac aac ctg cgc tac ggt gca 1171 Thr Gln Thr Glu Ala Gln Asp Leu Ala Asn Asn Leu Arg Tyr Gly Ala             345 350 355 ttg ccc ctg agc ttc gca ggt gaa aac ggc gag cgc ggc gga act acc 1219 Leu Pro Leu Ser Phe Ala Gly Glu Asn Gly Glu Arg Gly Gly Thr Thr         360 365 370 acc acc gtt ccg cca tca cta ggc gca gca tcc ttg aag gcc gga ctg 1267 Thr Thr Val Pro Pro Ser Leu Gly Ala Ala Ser Leu Lys Ala Gly Leu     375 380 385 atc gca ggc atc gtc ggc atc gcg ctg gtc gcc atc ttc gtg ttc gcc 1315 Ile Ala Gly Ile Val Gly Ile Ala Leu Val Ala Ile Phe Val Phe Ala 390 395 400 405 tac tac cgc gtc ttc gga ttc gtt tcc ctg ttc acc ctg ttt gcc gca 1363 Tyr Tyr Arg Val Phe Gly Phe Val Ser Leu Phe Thr Leu Phe Ala Ala                 410 415 420 ggc gtg ttg gtc tac ggc ctt ctg gta ctg ctg gga cgc tgg atc gga 1411 Gly Val Leu Val Tyr Gly Leu Leu Val Leu Leu Gly Arg Trp Ile Gly             425 430 435 tat tcc cta gac ctt gct ggt atc gcc ggt ttg atc atc ggt atc ggt 1459 Tyr Ser Leu Asp Leu Ala Gly Ile Ala Gly Leu Ile Ile Gly Ile Gly         440 445 450 acc acc gcc gac tcc ttc gtg gtg ttc tat gag cgc atc aag gat gag 1507 Thr Thr Ala Asp Ser Phe Val Val Phe Tyr Glu Arg Ile Lys Asp Glu     455 460 465 atc cgt gaa gga aga tcc ttt aga tct gca gta cct cgt gca tgg gaa 1555 Ile Arg Glu Gly Arg Ser Phe Arg Ser Ala Val Pro Arg Ala Trp Glu 470 475 480 485 agc gcc aag cgc acc atc gtc aca ggc aac atg gtc act ttg ctc ggc 1603 Ser Ala Lys Arg Thr Ile Val Thr Gly Asn Met Val Thr Leu Leu Gly                 490 495 500 gct atc gtg att tac ttg ctc gcg gtc ggc gaa gtc aag ggc ttt gcc 1651 Ala Ile Val Ile Tyr Leu Leu Ala Val Gly Glu Val Lys Gly Phe Ala             505 510 515 ttc acc ctg ggt ctg acc acc gta ttc gat ctc gtt gtc acc ttc ctg 1699 Phe Thr Leu Gly Leu Thr Thr Val Phe Asp Leu Val Val Thr Phe Leu         520 525 530 atc acg gca cca ctg gtt atc ctg gca tca cgc aac cca ttc ttt gcc 1747 Ile Thr Ala Pro Leu Val Ile Leu Ala Ser Arg Asn Pro Phe Phe Ala     535 540 545 aag tca tcg gtc aac ggc atg gga cga gtg atg aag ctc gtt gaa gaa 1795 Lys Ser Ser Val Asn Gly Met Gly Arg Val Met Lys Leu Val Glu Glu 550 555 560 565 cgc cgc gcc aac ggt gaa ttg gat gag cct gag tac ctg aaa aag atc 1843 Arg Arg Ala Asn Gly Glu Leu Asp Glu Pro Glu Tyr Leu Lys Lys Ile                 570 575 580 cat gcc aag aat gcg gca gct gat aag gct tcc act gac aat tct tcc 1891 His Ala Lys Asn Ala Ala Ala Asp Lys Ala Ser Thr Asp Asn Ser Ser             585 590 595 act gac aat tct gaa gca cct ggc acc gat acg aac caa gag gag gag 1939 Thr Asp Asn Ser Glu Ala Pro Gly Thr Asp Thr Asn Gln Glu Glu Glu         600 605 610 aag tagccatgac tgattcccag act 1965 Lys      <210> 58 <211> 614 <212> PRT <213> Corynebacterium glutamicum <400> 58 Val Leu Ile Val Val Gly Val Tyr Ala Leu Val Leu Leu Thr Gly Asp   1 5 10 15 Arg Ser Ala Thr Pro Lys Leu Gly Ile Asp Leu Gln Gly Gly Thr Arg              20 25 30 Val Thr Leu Val Pro Gln Gly Gln Asp Pro Thr Gln Asp Gln Leu Asn          35 40 45 Gln Ala Arg Thr Ile Leu Glu Asn Arg Val Asn Gly Met Gly Val Ser      50 55 60 Gly Ala Ser Val Val Ala Asp Gly Asn Thr Leu Val Ile Thr Val Pro  65 70 75 80 Gly Glu Asn Thr Ala Gln Ala Gln Ser Leu Gly Gln Thr Ser Gln Leu                  85 90 95 Leu Phe Arg Pro Val Gly Gln Ala Gly Met Pro Asp Met Thr Thr Leu             100 105 110 Met Pro Glu Leu Glu Glu Met Ala Asn Arg Trp Val Glu Tyr Gly Val         115 120 125 Ile Thr Glu Glu Gln Ala Asn Ala Ser Leu Glu Glu Met Asn Thr Ala     130 135 140 Val Ala Ser Thr Thr Ala Val Glu Gly Glu Glu Ala Thr Glu Pro Glu 145 150 155 160 Pro Val Thr Val Ser Ala Thr Pro Met Asp Glu Pro Ala Asn Ser Ile                 165 170 175 Glu Ala Thr Gln Arg Arg Gln Glu Ile Thr Asp Met Leu Arg Thr Asp             180 185 190 Arg Gln Ser Thr Asp Pro Thr Val Gln Ile Ala Ala Ser Ser Leu Met         195 200 205 Gln Cys Thr Thr Asp Glu Met Asp Pro Leu Ala Gly Thr Asp Asp Pro     210 215 220 Arg Leu Pro Leu Val Ala Cys Asp Pro Ala Val Gly Gly Val Tyr Val 225 230 235 240 Leu Asp Pro Ala Pro Leu Leu Asn Gly Glu Thr Asp Glu Glu Asn Gly                 245 250 255 Ala Arg Leu Thr Gly Asn Glu Ile Asp Thr Asn Arg Pro Ile Thr Gly             260 265 270 Gly Phe Asn Ala Gln Ser Gly Gln Met Glu Ile Ser Phe Ala Phe Lys         275 280 285 Ser Gly Asp Gly Glu Glu Gly Ser Ala Thr Trp Ser Ser Leu Thr Ser     290 295 300 Gln Tyr Leu Gln Gln Gln Ile Ala Ile Thr Leu Asp Ser Gln Val Ile 305 310 315 320 Ser Ala Pro Val Ile Gln Ser Ala Thr Pro Val Gly Ser Ala Thr Ser                 325 330 335 Ile Thr Gly Asp Phe Thr Gln Thr Glu Ala Gln Asp Leu Ala Asn Asn             340 345 350 Leu Arg Tyr Gly Ala Leu Pro Leu Ser Phe Ala Gly Glu Asn Gly Glu         355 360 365 Arg Gly Gly Thr Thr Thr Thr Thr Pro Pro Ser Leu Gly Ala Ala Ser     370 375 380 Leu Lys Ala Gly Leu Ile Ala Gly Ile Val Gly Ile Ala Leu Val Ala 385 390 395 400 Ile Phe Val Phe Ala Tyr Tyr Arg Val Phe Gly Phe Val Ser Leu Phe                 405 410 415 Thr Leu Phe Ala Ala Gly Val Leu Val Tyr Gly Leu Leu Val Leu Leu             420 425 430 Gly Arg Trp Ile Gly Tyr Ser Leu Asp Leu Ala Gly Ile Ala Gly Leu         435 440 445 Ile Ile Gly Ile Gly Thr Thr Ala Asp Ser Phe Val Val Phe Tyr Glu     450 455 460 Arg Ile Lys Asp Glu Ile Arg Glu Gly Arg Ser Phe Arg Ser Ala Val 465 470 475 480 Pro Arg Ala Trp Glu Ser Ala Lys Arg Thr Ile Val Thr Gly Asn Met                 485 490 495 Val Thr Leu Leu Gly Ala Ile Val Ile Tyr Leu Leu Ala Val Gly Glu             500 505 510 Val Lys Gly Phe Ala Phe Thr Leu Gly Leu Thr Thr Val Phe Asp Leu         515 520 525 Val Val Thr Phe Leu Ile Thr Ala Pro Leu Val Ile Leu Ala Ser Arg     530 535 540 Asn Pro Phe Phe Ala Lys Ser Ser Val Asn Gly Met Gly Arg Val Met 545 550 555 560 Lys Leu Val Glu Glu Arg Arg Ala Asn Gly Glu Leu Asp Glu Pro Glu                 565 570 575 Tyr Leu Lys Lys Ile His Ala Lys Asn Ala Ala Ala Asp Lys Ala Ser             580 585 590 Thr Asp Asn Ser Ser Thr Asp Asn Ser Glu Ala Pro Gly Thr Asp Thr         595 600 605 Asn Gln Glu Glu Glu Lys     610 <210> 59 <211> 819 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (796) <223> RXN00046 <400> 59 tggtgcccaa gcagccgtca tcgcagcagc aaaatatgcc cgcgataacg ccttttaagc 60 acctaaaacg ctgttctcaa cacaggagtt tccttaaata atg gac tta aat act 115                                             Met Asp Leu Asn Thr                                               1 5 caa cgc tca aag ctc tac gca cag ctt caa ggc cag ctc att gtt tcc 163 Gln Arg Ser Lys Leu Tyr Ala Gln Leu Gln Gly Gln Leu Ile Val Ser                  10 15 20 gtg caa gct ccc gac ggc cat gcc atg cga gat acc cat acg ctc acc 211 Val Gln Ala Pro Asp Gly His Ala Met Arg Asp Thr His Thr Leu Thr              25 30 35 cat gtg gcc gca gcc tgt gtc gat ggc ggt gct cct gcc att cgc tgt 259 His Val Ala Ala Ala Cys Val Asp Gly Gly Ala Pro Ala Ile Arg Cys          40 45 50 ggc ggt tac ggc ggt ttg gaa gat atc cgt tca atc tcc aac cgt gtc 307 Gly Gly Tyr Gly Gly Leu Glu Asp Ile Arg Ser Ile Ser Asn Arg Val      55 60 65 gac gtt ccc gtt ttc gga ctc acc aaa gaa ggc tcc gaa gga gtt tac 355 Asp Val Pro Val Phe Gly Leu Thr Lys Glu Gly Ser Glu Gly Val Tyr  70 75 80 85 atc acc cca acc agg gat tcc gtt cga gca gtg gca gaa tcc ggc gcc 403 Ile Thr Pro Thr Arg Asp Ser Val Arg Ala Val Ala Glu Ser Gly Ala                  90 95 100 act gta gtc tgc gcg gat gca act ttc cga cct agg cct gac ggc tcc 451 Thr Val Val Cys Ala Asp Ala Thr Phe Arg Pro Arg Pro Asp Gly Ser             105 110 115 acc ttt gca gag ctg gtc act gtt gcc cac gat tcc gga att ctc atc 499 Thr Phe Ala Glu Leu Val Thr Val Ala His Asp Ser Gly Ile Leu Ile         120 125 130 atg gcg gac tgc gca act ccc gaa gaa gtt ctc agt gcg cat aag gct 547 Met Ala Asp Cys Ala Thr Pro Glu Glu Val Leu Ser Ala His Lys Ala     135 140 145 ggc gcg gat ttt gtg tcc acc acg ctt gct gga tac acc gaa cac cgc 595 Gly Ala Asp Phe Val Ser Thr Thr Leu Ala Gly Tyr Thr Glu His Arg 150 155 160 165 gag aaa aca gtc ggt cca gat ttc gat tgc ctc cgc gaa gca cgt gag 643 Glu Lys Thr Val Gly Pro Asp Phe Asp Cys Leu Arg Glu Ala Arg Glu                 170 175 180 tta gtt ccc gat gcg ttc ctc att ggc gaa ggt cgc ttc tcc aac cct 691 Leu Val Pro Asp Ala Phe Leu Ile Gly Glu Gly Arg Phe Ser Asn Pro             185 190 195 gcg gat gtg gcg cac ggt cgt ctc att ggt gcc aac gcg atc atc gtg 739 Ala Asp Val Ala His Gly Arg Leu Ile Gly Ala Asn Ala Ile Ile Val         200 205 210 ggc acc gca atc act gac cct ggt ttc atc act gga cag ttc gcg tca 787 Gly Thr Ala Ile Thr Asp Pro Gly Phe Ile Thr Gly Gln Phe Ala Ser     215 220 225 ctg ttg cac tagcacttag tccagcgctg cac 819 Leu Leu His 230 <210> 60 <211> 232 <212> PRT <213> Corynebacterium glutamicum <400> 60 Met Asp Leu Asn Thr Gln Arg Ser Lys Leu Tyr Ala Gln Leu Gln Gly   1 5 10 15 Gln Leu Ile Val Ser Val Gln Ala Pro Asp Gly His Ala Met Arg Asp              20 25 30 Thr His Thr Leu Thr His Val Ala Ala Ala Cys Val Asp Gly Gly Ala          35 40 45 Pro Ala Ile Arg Cys Gly Gly Tyr Gly Gly Leu Glu Asp Ile Arg Ser      50 55 60 Ile Ser Asn Arg Val Asp Val Pro Val Phe Gly Leu Thr Lys Glu Gly  65 70 75 80 Ser Glu Gly Val Tyr Ile Thr Pro Thr Arg Asp Ser Val Arg Ala Val                  85 90 95 Ala Glu Ser Gly Ala Thr Val Val Cys Ala Asp Ala Thr Phe Arg Pro             100 105 110 Arg Pro Asp Gly Ser Thr Phe Ala Glu Leu Val Thr Val Ala His Asp         115 120 125 Ser Gly Ile Leu Ile Met Ala Asp Cys Ala Thr Pro Glu Glu Val Leu     130 135 140 Ser Ala His Lys Ala Gly Ala Asp Phe Val Ser Thr Thr Leu Ala Gly 145 150 155 160 Tyr Thr Glu His Arg Glu Lys Thr Val Gly Pro Asp Phe Asp Cys Leu                 165 170 175 Arg Glu Ala Arg Glu Leu Val Pro Asp Ala Phe Leu Ile Gly Glu Gly             180 185 190 Arg Phe Ser Asn Pro Ala Asp Val Ala His Gly Arg Leu Ile Gly Ala         195 200 205 Asn Ala Ile Ile Val Gly Thr Ala Ile Thr Asp Pro Gly Phe Ile Thr     210 215 220 Gly Gln Phe Ala Ser Leu Leu His 225 230 <210> 61 <211> 1219 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (48) .. (1196) <223> RXN01863 <400> 61 ggtatcatac cgatatgaac caaatagaaa gaaggaagtt taagacg atg aat agc 56                                                     Met asn ser                                                      One gtc aaa ttg aag caa cct gtt agc att tac aat gat cca tgg gaa tca 104 Val Lys Leu Lys Gln Pro Val Ser Ile Tyr Asn Asp Pro Trp Glu Ser       5 10 15 tat aac gat gtt aaa gaa cat ggc caa tta act tta agt aac atc gaa 152 Tyr Asn Asp Val Lys Glu His Gly Gln Leu Thr Leu Ser Asn Ile Glu  20 25 30 35 ttt aca act aca aat ctt tgt aat atg cgt tgt agc cac tgt gca gtt 200 Phe Thr Thr Thr Asn Leu Cys Asn Met Arg Cys Ser His Cys Ala Val                  40 45 50 ggt tat act tta caa act gtc gac ccc gag cct tta gat atg gac tta 248 Gly Tyr Thr Leu Gln Thr Val Asp Pro Glu Pro Leu Asp Met Asp Leu              55 60 65 att tat cgt aga ctt gat gaa att cca aat ctg cga acg atg tca att 296 Ile Tyr Arg Arg Leu Asp Glu Ile Pro Asn Leu Arg Thr Met Ser Ile          70 75 80 aca ggt ggc gaa cca atg ttt tct aaa aag tct att aga aat gtt gtt 344 Thr Gly Gly Glu Pro Met Phe Ser Lys Lys Ser Ile Arg Asn Val Val      85 90 95 aaa cct cta tta aag tat gca cat cat cga ggt ata tat aca caa atg 392 Lys Pro Leu Leu Lys Tyr Ala His His Arg Gly Ile Tyr Thr Gln Met 100 105 110 115 aat tca aac cta aca ttg cct caa gat cgt tat tta gat att gct gaa 440 Asn Ser Asn Leu Thr Leu Pro Gln Asp Arg Tyr Leu Asp Ile Ala Glu                 120 125 130 tat atc gat gtt atg cat atc tca cat aac tgg gga aca act gat gaa 488 Tyr Ile Asp Val Met His Ile Ser His Asn Trp Gly Thr Thr Asp Glu             135 140 145 ttc gca aat gtt ggc ttt ggc gca atg aag aag caa cca ccg tta aaa 536 Phe Ala Asn Val Gly Phe Gly Ala Met Lys Lys Gln Pro Pro Leu Lys         150 155 160 gct aag tta aaa tta tat gaa caa atg att tcg aat gca cgt aca tta 584 Ala Lys Leu Lys Leu Tyr Glu Gln Met Ile Ser Asn Ala Arg Thr Leu     165 170 175 tca gaa caa gga atg ttt gta tct gcg gaa aca atg ctc aat caa agt 632 Ser Glu Gln Gly Met Phe Val Ser Ala Glu Thr Met Leu Asn Gln Ser 180 185 190 195 acg cta cca cat tta cga aaa ata cat caa gaa gtc gtt cat gat atg 680 Thr Leu Pro His Leu Arg Lys Ile His Gln Glu Val Val His Asp Met                 200 205 210 aaa tgt agc aga cac gag att cac cct atg tat cca gct gac ttt gca 728 Lys Cys Ser Arg His Glu Ile His Pro Met Tyr Pro Ala Asp Phe Ala             215 220 225 agt caa tta aat gtg tta act cta gcg gaa atg aaa aag aca att cat 776 Ser Gln Leu Asn Val Leu Thr Leu Ala Glu Met Lys Lys Thr Ile His         230 235 240 gat ata ttg gat ttc aga gat gaa gat att tgg atg tta ttt ggt act 824 Asp Ile Leu Asp Phe Arg Asp Glu Asp Ile Trp Met Leu Phe Gly Thr     245 250 255 ttg cct gtg ttt cca tgc tta aag gat gat gaa gat caa aag tta cta 872 Leu Pro Val Phe Pro Cys Leu Lys Asp Asp Glu Asp Gln Lys Leu Leu 260 265 270 275 tca cgt tta aga aat gct aac aat gta acg act aga aat gac ccg gat 920 Ser Arg Leu Arg Asn Ala Asn Asn Val Thr Thr Arg Asn Asp Pro Asp                 280 285 290 ggc cgt agt cgt tta aat gtc aat gta ttt aca ggt aat gta atc gta 968 Gly Arg Ser Arg Leu Asn Val Asn Val Phe Thr Gly Asn Val Ile Val             295 300 305 act gat ttc gga gat gaa aca ggt aca att tcg aat ata caa aaa gat 1016 Thr Asp Phe Gly Asp Glu Thr Gly Thr Ile Ser Asn Ile Gln Lys Asp         310 315 320 aaa tta aca gat gta ttt gat aaa tgg tta tcc tct gat ctt gct aaa 1064 Lys Leu Thr Asp Val Phe Asp Lys Trp Leu Ser Ser Asp Leu Ala Lys     325 330 335 tca tta aat tgt cat tgt tcc gag ttt agt tgt tta gga cca aat gtt 1112 Ser Leu Asn Cys His Cys Ser Glu Phe Ser Cys Leu Gly Pro Asn Val 340 345 350 355 ctt gtt aaa aat atg tac tat ccg aat atg gat ttt aaa gat aat gag 1160 Leu Val Lys Asn Met Tyr Tyr Pro Asn Met Asp Phe Lys Asp Asn Glu                 360 365 370 cgt cat atg cac aaa caa cca caa att ata caa ttt taaaaactct 1206 Arg His Met His Lys Gln Pro Gln Ile Ile Gln Phe             375 380 taattatgcg gag 1219 <210> 62 <211> 383 <212> PRT <213> Corynebacterium glutamicum <400> 62 Met Asn Ser Val Lys Leu Lys Gln Pro Val Ser Ile Tyr Asn Asp Pro   1 5 10 15 Trp Glu Ser Tyr Asn Asp Val Lys Glu His Gly Gln Leu Thr Leu Ser              20 25 30 Asn Ile Glu Phe Thr Thr Thr Asn Leu Cys Asn Met Arg Cys Ser His          35 40 45 Cys Ala Val Gly Tyr Thr Leu Gln Thr Val Asp Pro Glu Pro Leu Asp      50 55 60 Met Asp Leu Ile Tyr Arg Arg Leu Asp Glu Ile Pro Asn Leu Arg Thr  65 70 75 80 Met Ser Ile Thr Gly Gly Glu Pro Met Phe Ser Lys Lys Ser Ile Arg                  85 90 95 Asn Val Val Lys Pro Leu Leu Lys Tyr Ala His His Arg Gly Ile Tyr             100 105 110 Thr Gln Met Asn Ser Asn Leu Thr Leu Pro Gln Asp Arg Tyr Leu Asp         115 120 125 Ile Ala Glu Tyr Ile Asp Val Met His Ile Ser His Asn Trp Gly Thr     130 135 140 Thr Asp Glu Phe Ala Asn Val Gly Phe Gly Ala Met Lys Lys Gln Pro 145 150 155 160 Pro Leu Lys Ala Lys Leu Lys Leu Tyr Glu Gln Met Ile Ser Asn Ala                 165 170 175 Arg Thr Leu Ser Glu Gln Gly Met Phe Val Ser Ala Glu Thr Met Leu             180 185 190 Asn Gln Ser Thr Leu Pro His Leu Arg Lys Ile His Gln Glu Val Val         195 200 205 His Asp Met Lys Cys Ser Arg His Glu Ile His Pro Met Tyr Pro Ala     210 215 220 Asp Phe Ala Ser Gln Leu Asn Val Leu Thr Leu Ala Glu Met Lys Lys 225 230 235 240 Thr Ile His Asp Ile Leu Asp Phe Arg Asp Glu Asp Ile Trp Met Leu                 245 250 255 Phe Gly Thr Leu Pro Val Phe Pro Cys Leu Lys Asp Asp Glu Asp Gln             260 265 270 Lys Leu Leu Ser Arg Leu Arg Asn Ala Asn Asn Val Thr Thr Arg Asn         275 280 285 Asp Pro Asp Gly Arg Ser Arg Leu Asn Val Asn Val Phe Thr Gly Asn     290 295 300 Val Ile Val Thr Asp Phe Gly Asp Glu Thr Gly Thr Ile Ser Asn Ile 305 310 315 320 Gln Lys Asp Lys Leu Thr Asp Val Phe Asp Lys Trp Leu Ser Ser Asp                 325 330 335 Leu Ala Lys Ser Leu Asn Cys His Cys Ser Glu Phe Ser Cys Leu Gly             340 345 350 Pro Asn Val Leu Val Lys Asn Met Tyr Tyr Pro Asn Met Asp Phe Lys         355 360 365 Asp Asn Glu Arg His Met His Lys Gln Pro Gln Ile Ile Gln Phe     370 375 380 <210> 63 <211> 618 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (101) .. (595) <223> RXN00833 <400> 63 agctttttgc atgtgtcata tcgtaccgtt tgcataggcc tgttcgcgct tggtgaacct 60 tttctagcac caaaacaaaa ctctccctag tatggggtcc atg gct aaa aca cat 115                                             Met Ala Lys Thr His                                               1 5 ttt caa ggc aac gaa act gct acc tcc ggc gaa ctg cca cag gtc ggc 163 Phe Gln Gly Asn Glu Thr Ala Thr Ser Gly Glu Leu Pro Gln Val Gly                  10 15 20 gac aac ctc gca gag ttc aac ctc gtc aac acc gaa ctg ggc gag gtc 211 Asp Asn Leu Ala Glu Phe Asn Leu Val Asn Thr Glu Leu Gly Glu Val              25 30 35 tcc tca aag gac ttc cag ggc cgc aag ctt gtc ctg aac atc ttc cca 259 Ser Ser Lys Asp Phe Gln Gly Arg Lys Leu Val Leu Asn Ile Phe Pro          40 45 50 tcc gtt gac acc ggc gtt tgt gca aca tca gtc cgc aag ttc aac gag 307 Ser Val Asp Thr Gly Val Cys Ala Thr Ser Val Arg Lys Phe Asn Glu      55 60 65 gca gca gca agc ctg gaa aac acc acc gtg ctg tgc atc tcc aag gat 355 Ala Ala Ala Ser Leu Glu Asn Thr Thr Val Val Leu Cys Ile Ser Lys Asp  70 75 80 85 ctt cca ttc gca ctg ggc cgt ttc tgc tcc gca gaa ggc atc gag aac 403 Leu Pro Phe Ala Leu Gly Arg Phe Cys Ser Ala Glu Gly Ile Glu Asn                  90 95 100 gtc acc cca gta tcc gca ttc cgt tcc acc ttc ggt gaa gac aac ggc 451 Val Thr Pro Val Ser Ala Phe Arg Ser Thr Phe Gly Glu Asp Asn Gly             105 110 115 atc gtg ctc gaa ggc tca cca ctt aag ggt ctt ctt gca cgc agc gtc 499 Ile Val Leu Glu Gly Ser Pro Leu Lys Gly Leu Leu Ala Arg Ser Val         120 125 130 atc gtc gtc gat gaa aac ggc aag gtt gct tac acc cag ttg gtt gat 547 Ile Val Val Asp Glu Asn Gly Lys Val Ala Tyr Thr Gln Leu Val Asp     135 140 145 gag atc ttc act gaa cct gat tac gac gct gca ctt gct ggg ctg aac 595 Glu Ile Phe Thr Glu Pro Asp Tyr Asp Ala Ala Leu Ala Gly Leu Asn 150 155 160 165 taatttactt cgctcagggg aat 618 <210> 64 <211> 165 <212> PRT <213> Corynebacterium glutamicum <400> 64 Met Ala Lys Thr His Phe Gln Gly Asn Glu Thr Ala Thr Ser Gly Glu   1 5 10 15 Leu Pro Gln Val Gly Asp Asn Leu Ala Glu Phe Asn Leu Val Asn Thr              20 25 30 Glu Leu Gly Glu Val Ser Ser Lys Asp Phe Gln Gly Arg Lys Leu Val          35 40 45 Leu Asn Ile Phe Pro Ser Val Asp Thr Gly Val Cys Ala Thr Ser Val      50 55 60 Arg Lys Phe Asn Glu Ala Ala Ala Ser Leu Glu Asn Thr Thr Val Leu  65 70 75 80 Cys Ile Ser Lys Asp Leu Pro Phe Ala Leu Gly Arg Phe Cys Ser Ala                  85 90 95 Glu Gly Ile Glu Asn Val Thr Pro Val Ser Ala Phe Arg Ser Thr Phe             100 105 110 Gly Glu Asp Asn Gly Ile Val Leu Glu Gly Ser Pro Leu Lys Gly Leu         115 120 125 Leu Ala Arg Ser Val Ile Val Val Asp Glu Asn Gly Lys Val Ala Tyr     130 135 140 Thr Gln Leu Val Asp Glu Ile Phe Thr Glu Pro Asp Tyr Asp Ala Ala 145 150 155 160 Leu Ala Gly Leu Asn                 165 <210> 65 <211> 879 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (856) <223> RXN01676 <400> 65 agttacagct tttctcggtg gcacactcgc gctacttagc ccttgtgccg cactcctttt 60 accagcattt tttgcatcct cagtgggtgc tggcccgcgc atg atc ctt cac ggt 115                                             Met Ile Leu His Gly                                               1 5 gtt gtg ttc tac gca gga ctt cta gta ctt ctc gtg cca ctt ggc ctt 163 Val Val Phe Tyr Ala Gly Leu Leu Val Leu Leu Val Pro Leu Gly Leu                  10 15 20 ggt gcg gga atc ctc ggc gag ctg ttt atc acc caa cgc cag acc atc 211 Gly Ala Gly Ile Leu Gly Glu Leu Phe Ile Thr Gln Arg Gln Thr Ile              25 30 35 atc gtg gtt tca tcg atc gtg ctg att atc cta ggt ttt gtc cag atc 259 Ile Val Val Ser Ser Ile Val Leu Ile Ile Leu Gly Phe Val Gln Ile          40 45 50 ttc ggc ggc gga ttc gac ttc gga aaa gca ctc cca gga tta gat cgt 307 Phe Gly Gly Gly Phe Asp Phe Gly Lys Ala Leu Pro Gly Leu Asp Arg      55 60 65 ctg caa tct aag gcc act gtg acc tca ggt cta gga aag agc ttt tta 355 Leu Gln Ser Lys Ala Thr Val Thr Ser Gly Leu Gly Lys Ser Phe Leu  70 75 80 85 cta gga atg acc agt agt att gcc ggt ttt tgt tcc gga cca atc ctc 403 Leu Gly Met Thr Ser Ser Ile Ala Gly Phe Cys Ser Gly Pro Ile Leu                  90 95 100 ggc gcc gtt ctt act ttg gct gcc acc agt gga aac tcc atc acc tca 451 Gly Ala Val Leu Thr Leu Ala Ala Thr Ser Gly Asn Ser Ile Thr Ser             105 110 115 gca ctc att ttg agt gct tat ggt gcg gga atg gtg ctg ccc ctg atg 499 Ala Leu Ile Leu Ser Ala Tyr Gly Ala Gly Met Val Leu Pro Leu Met         120 125 130 gct att gca gcg ctc tgg gcc aaa ctc gga cag cgt gga cag cag atg 547 Ala Ile Ala Ala Leu Trp Ala Lys Leu Gly Gln Arg Gly Gln Gln Met     135 140 145 ctc cgc ggc cgg gaa ttc acc ttc ttg ggc agg cag tgg cac att gtt 595 Leu Arg Gly Arg Glu Phe Thr Phe Leu Gly Arg Gln Trp His Ile Val 150 155 160 165 tct gtc att agc ggt gcc ctg atc atc gct gtc gga atc ctc ttt tgg 643 Ser Val Ile Ser Gly Ala Leu Ile Ile Ala Val Gly Ile Leu Phe Trp                 170 175 180 tcc acg aac ggc ctt gtc agc atg ccg gag ctc gtt cca atg gac acc 691 Ser Thr Asn Gly Leu Val Ser Met Pro Glu Leu Val Pro Met Asp Thr             185 190 195 cag atc tgg cta cag gaa gcc aca ttc tca ctc ggg tca cca ctc ttt 739 Gln Ile Trp Leu Gln Glu Ala Thr Phe Ser Leu Gly Ser Pro Leu Phe         200 205 210 gac atc gca ttg atc att gtc gcc gct ggc ttg ttc ttg tac ttc tgg 787 Asp Ile Ala Leu Ile Ile Val Ala Ala Gly Leu Phe Leu Tyr Phe Trp     215 220 225 aac aaa cga caa aag cga aaa gaa gaa gct cag cga ccc aaa gaa agt 835 Asn Lys Arg Gln Lys Arg Lys Glu Glu Ala Gln Arg Pro Lys Glu Ser 230 235 240 245 gga tgg gtt att aac cct cgc taattattag ttttggagcg agg 879 Gly Trp Val Ile Asn Pro Arg                 250 <210> 66 <211> 252 <212> PRT <213> Corynebacterium glutamicum <400> 66 Met Ile Leu His Gly Val Val Phe Tyr Ala Gly Leu Leu Val Leu Leu   1 5 10 15 Val Pro Leu Gly Leu Gly Ala Gly Ile Leu Gly Glu Leu Phe Ile Thr              20 25 30 Gln Arg Gln Thr Ile Ile Val Val Ser Ser Ile Val Leu Ile Ile Leu          35 40 45 Gly Phe Val Gln Ile Phe Gly Gly Gly Phe Asp Phe Gly Lys Ala Leu      50 55 60 Pro Gly Leu Asp Arg Leu Gln Ser Lys Ala Thr Val Thr Ser Gly Leu  65 70 75 80 Gly Lys Ser Phe Leu Leu Gly Met Thr Ser Ser Ile Ala Gly Phe Cys                  85 90 95 Ser Gly Pro Ile Leu Gly Ala Val Leu Thr Leu Ala Ala Thr Ser Gly             100 105 110 Asn Ser Ile Thr Ser Ala Leu Ile Leu Ser Ala Tyr Gly Ala Gly Met         115 120 125 Val Leu Pro Leu Met Ala Ile Ala Ala Leu Trp Ala Lys Leu Gly Gln     130 135 140 Arg Gly Gln Gln Met Leu Arg Gly Arg Glu Phe Thr Phe Leu Gly Arg 145 150 155 160 Gln Trp His Ile Val Ser Val Ile Ser Gly Ala Leu Ile Ile Ala Val                 165 170 175 Gly Ile Leu Phe Trp Ser Thr Asn Gly Leu Val Ser Met Pro Glu Leu             180 185 190 Val Pro Met Asp Thr Gln Ile Trp Leu Gln Glu Ala Thr Phe Ser Leu         195 200 205 Gly Ser Pro Leu Phe Asp Ile Ala Leu Ile Ile Val Ala Ala Gly Leu     210 215 220 Phe Leu Tyr Phe Trp Asn Lys Arg Gln Lys Arg Lys Glu Glu Ala Gln 225 230 235 240 Arg Pro Lys Glu Ser Gly Trp Val Ile Asn Pro Arg                 245 250 <210> 67 <211> 744 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (721) <223> RXN00380 <400> 67 caggcaatgc gacctcgcct cagtgacatc cttggtgttc caagacgatc aaattgtcgg 60 cgtgcattac aacgaaccag ctcaggagat ttgatcactc gtg cgt ttg acc aaa 115                                             Val Arg Leu Thr Lys                                               1 5 cta gca gca aca atc ggc tgc gtg aca ctc agc gga ctt gcg cta gta 163 Leu Ala Ala Thr Ile Gly Cys Val Thr Leu Ser Gly Leu Ala Leu Val                  10 15 20 gcc tgc agc agt gac agt acc gct ggt act gac gct gtt gct gtc ggc 211 Ala Cys Ser Ser Asp Ser Thr Ala Gly Thr Asp Ala Val Ala Val Gly              25 30 35 gga acc ttc caa ttc cac tcc ccg gat gga aag atg gaa att ttc tac 259 Gly Thr Phe Gln Phe His Ser Pro Asp Gly Lys Met Glu Ile Phe Tyr          40 45 50 gac gag gct gac cgt caa caa ctc ccc gac att ggt gga gat tcc ctc 307 Asp Glu Ala Asp Arg Gln Gln Leu Pro Asp Ile Gly Gly Asp Ser Leu      55 60 65 atg gaa gag ggc aca cag atc aac ctg tct gat ttc gaa aac caa gtt 355 Met Glu Glu Gly Thr Gln Ile Asn Leu Ser Asp Phe Glu Asn Gln Val  70 75 80 85 gtc atc ctc aat gcg tgg ggg cag tgg tgt gca ccg tgc cgc tcc gaa 403 Val Ile Leu Asn Ala Trp Gly Gln Trp Cys Ala Pro Cys Arg Ser Glu                  90 95 100 tcc gat gat ctc cag att atc cat gag gaa ctc caa gct gcc gga aac 451 Ser Asp Asp Leu Gln Ile Ile His Glu Glu Leu Gln Ala Ala Gly Asn             105 110 115 ggc gac acc cct ggt ggc acc gtg ttg ggt atc aat gtg cgt gat tac 499 Gly Asp Thr Pro Gly Gly Thr Val Leu Gly Ile Asn Val Arg Asp Tyr         120 125 130 tcc cgc gac atc gcc caa gac ttt gtc acc gac aac ggc ctt gat tac 547 Ser Arg Asp Ile Ala Gln Asp Phe Val Thr Asp Asn Gly Leu Asp Tyr     135 140 145 cca agc att tac gat cca cca ttt atg aca gca gca tcc ctc ggt ggt 595 Pro Ser Ile Tyr Asp Pro Pro Phe Met Thr Ala Ala Ser Leu Gly Gly 150 155 160 165 gtt ccc gca tcg gtg atc cca acc acc atc gtg ctg gat aaa cag cac 643 Val Pro Ala Ser Val Ile Pro Thr Thr Ile Val Leu Asp Lys Gln His                 170 175 180 cgc ccc gca gca gtg ttc ttg cgc gaa gtc acc tcc aaa gat gtg ttg 691 Arg Pro Ala Ala Val Phe Leu Arg Glu Val Thr Ser Lys Asp Val Leu             185 190 195 gat gtt gcg ttg cca ttg gta gat gag gcc taaatgtctg agattgtggt 741 Asp Val Ala Leu Pro Leu Val Asp Glu Ala         200 205 agc 744 <210> 68 <211> 207 <212> PRT <213> Corynebacterium glutamicum <400> 68 Val Arg Leu Thr Lys Leu Ala Ala Thr Ile Gly Cys Val Thr Leu Ser   1 5 10 15 Gly Leu Ala Leu Val Ala Cys Ser Ser Asp Ser Thr Ala Gly Thr Asp              20 25 30 Ala Val Ala Val Gly Gly Thr Phe Gln Phe His Ser Pro Asp Gly Lys          35 40 45 Met Glu Ile Phe Tyr Asp Glu Ala Asp Arg Gln Gln Leu Pro Asp Ile      50 55 60 Gly Gly Asp Ser Leu Met Glu Glu Gly Thr Gln Ile Asn Leu Ser Asp  65 70 75 80 Phe Glu Asn Gln Val Val Ile Leu Asn Ala Trp Gly Gln Trp Cys Ala                  85 90 95 Pro Cys Arg Ser Glu Ser Asp Asp Leu Gln Ile Ile His Glu Glu Leu             100 105 110 Gln Ala Ala Gly Asn Gly Asp Thr Pro Gly Gly Thr Val Leu Gly Ile         115 120 125 Asn Val Arg Asp Tyr Ser Arg Asp Ile Ala Gln Asp Phe Val Thr Asp     130 135 140 Asn Gly Leu Asp Tyr Pro Ser Ile Tyr Asp Pro Pro Phe Met Thr Ala 145 150 155 160 Ala Ser Leu Gly Gly Val Pro Ala Ser Val Ile Pro Thr Thr Ile Val                 165 170 175 Leu Asp Lys Gln His Arg Pro Ala Ala Val Phe Leu Arg Glu Val Thr             180 185 190 Ser Lys Asp Val Leu Asp Val Ala Leu Pro Leu Val Asp Glu Ala         195 200 205 <210> 69 <211> 495 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (472) <223> RXN00937 <400> 69 agctgccggt caatgaagaa aatccttggc cgggaataac tacagtccgc tgaaagttgg 60 tctatatata gaccttacaa atcttgaacg gagattctta atg gca acc atc gat 115                                             Met Ala Thr Ile Asp                                               1 5 gta acc gaa gaa aca ttt gag agc aca gtt acc ggc gac gga att gtc 163 Val Thr Glu Glu Thr Phe Glu Ser Thr Val Thr Gly Asp Gly Ile Val                  10 15 20 ctc gta gac gca tgg gca tcc tgg tgc gga cct tgc cgc cag ttc gcc 211 Leu Val Asp Ala Trp Ala Ser Trp Cys Gly Pro Cys Arg Gln Phe Ala              25 30 35 cca acc tac gag aag gtt tcc gaa acc cac acc gac gca acc ttc gcc 259 Pro Thr Tyr Glu Lys Val Ser Glu Thr His Thr Asp Ala Thr Phe Ala          40 45 50 aag ctt gat acc gaa gca aac cag ggc ctg gct gca gca ctg cag atc 307 Lys Leu Asp Thr Glu Ala Asn Gln Gly Leu Ala Ala Ala Leu Gln Ile      55 60 65 cag tcc atc cca act ctg atg gtt ttc cgc gac ggc atc atg gtc tac 355 Gln Ser Ile Pro Thr Leu Met Val Phe Arg Asp Gly Ile Met Val Tyr  70 75 80 85 cgc gaa gcc ggc acc atg cca gct cct gca ctg gat gat ctg gtc aac 403 Arg Glu Ala Gly Thr Met Pro Ala Pro Ala Leu Asp Asp Leu Val Asn                  90 95 100 cag gtt aag gca ctc gac atg gat gac gtt cgt cgc cag gtc gca gag 451 Gln Val Lys Ala Leu Asp Met Asp Asp Val Arg Arg Gln Val Ala Glu             105 110 115 cag cag ggt tct gca gag gca taagcttcca attgtgtttt ggt 495 Gln Gln Gly Ser Ala Glu Ala         120 <210> 70 <211> 124 <212> PRT <213> Corynebacterium glutamicum <400> 70 Met Ala Thr Ile Asp Val Thr Glu Glu Thr Phe Glu Ser Thr Val Thr   1 5 10 15 Gly Asp Gly Ile Val Leu Val Asp Ala Trp Ala Ser Trp Cys Gly Pro              20 25 30 Cys Arg Gln Phe Ala Pro Thr Tyr Glu Lys Val Ser Glu Thr His Thr          35 40 45 Asp Ala Thr Phe Ala Lys Leu Asp Thr Glu Ala Asn Gln Gly Leu Ala      50 55 60 Ala Ala Leu Gln Ile Gln Ser Ile Pro Thr Leu Met Val Phe Arg Asp  65 70 75 80 Gly Ile Met Val Tyr Arg Glu Ala Gly Thr Met Pro Ala Pro Ala Leu                  85 90 95 Asp Asp Leu Val Asn Gln Val Lys Ala Leu Asp Met Asp Asp Val Arg             100 105 110 Arg Gln Val Ala Glu Gln Gln Gly Ser Ala Glu Ala         115 120 <210> 71 <211> 990 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (967) <223> RXN02325 <400> 71 cagagatttg aagatggaga ccaaggctca aagggaatcc atgccgtctt ggtttaatac 60 tgcacccgtc taatgaaaat cattactatt aggtgtcatg atg gac cat gca cac 115                                             Met Asp His Ala His                                               1 5 gat tcc tgc tca cca act ctg cgc cgt gat ttg gag gtc act ggc cag 163 Asp Ser Cys Ser Pro Thr Leu Arg Arg Asp Leu Glu Val Thr Gly Gln                  10 15 20 ctc caa cct gag aaa gct gtc gat tta gca gcg ccg cac gaa ggg aag 211 Leu Gln Pro Glu Lys Ala Val Asp Leu Ala Ala Pro His Glu Gly Lys              25 30 35 gtt gcc aat ata acg aag gtg acc tcc tca aat atg gag cac acc atc 259 Val Ala Asn Ile Thr Lys Val Thr Ser Ser Asn Met Glu His Thr Ile          40 45 50 acg cag gcc tca aaa gct aag gag gtg gtg gtg ctc att ggt cac tcc 307 Thr Gln Ala Ser Lys Ala Lys Glu Val Val Val Leu Ile Gly His Ser      55 60 65 ctg ctg ccc aca ttt cag gat ttg gaa aaa gac att ctg cac ttt cag 355 Leu Leu Pro Thr Phe Gln Asp Leu Glu Lys Asp Ile Leu His Phe Gln  70 75 80 85 gca ggt aat aaa ggg cga ttt tct gta gcg att gtt gat cct gat cgc 403 Ala Gly Asn Lys Gly Arg Phe Ser Val Ala Ile Val Asp Pro Asp Arg                  90 95 100 agt gca gat gtg gtt gcc aga ttt agg cca aaa cag att ccg gtg gca 451 Ser Ala Asp Val Val Ala Arg Phe Arg Pro Lys Gln Ile Pro Val Ala             105 110 115 tac gtg gtg aaa gat ggc gcc agc att gcg gag ttc aac tcg ctc aac 499 Tyr Val Val Lys Asp Gly Ala Ser Ile Ala Glu Phe Asn Ser Leu Asn         120 125 130 aag gag ccg gtt gca caa tgg ctt gat cat ttt gtg tcg cgg gaa acg 547 Lys Glu Pro Val Ala Gln Trp Leu Asp His Phe Val Ser Arg Glu Thr     135 140 145 atc ccc aat gaa aaa gag ggg gac gtc gat aag caa ata gac ccg cgc 595 Ile Pro Asn Glu Lys Glu Gly Asp Val Asp Lys Gln Ile Asp Pro Arg 150 155 160 165 ctg tgg cgg gca gcg gaa ttg gtg aac gcc ggt gat ttt cgc gcg gcg 643 Leu Trp Arg Ala Ala Glu Leu Val Asn Ala Gly Asp Phe Arg Ala Ala                 170 175 180 ttg gcg ttg tat gag cag ttg ccg cag gat gcg acg gtg aag cgg gcg 691 Leu Ala Leu Tyr Glu Gln Leu Pro Gln Asp Ala Thr Val Lys Arg Ala             185 190 195 cac gcg gcg gtg tcg gta ttg gcg cgg atg tct gtg gcg gat cgg gga 739 His Ala Ala Val Ser Val Leu Ala Arg Met Ser Val Ala Asp Arg Gly         200 205 210 gag gat ccg atc gag aag tcg cgc cgg gat cca gac gat gtg aac aag 787 Glu Asp Pro Ile Glu Lys Ser Arg Arg Asp Pro Asp Asp Val Asn Lys     215 220 225 gcg ctg gcg gcg gcg gat atg tat gtg ttg atg aat cag ccg gac aca 835 Ala Leu Ala Ala Ala Asp Met Tyr Val Leu Met Asn Gln Pro Asp Thr 230 235 240 245 gcg ctc gcg cac ctt gca gca cta ttg cca aaa ccg gag gct gcc cgg 883 Ala Leu Ala His Leu Ala Ala Leu Leu Pro Lys Pro Glu Ala Ala Arg                 250 255 260 cgg atc gtg gag ttg ctg aac ttg ttt gat ccg ctg gac ctg gtc gca 931 Arg Ile Val Glu Leu Leu Asn Leu Phe Asp Pro Leu Asp Leu Val Ala             265 270 275 ttg gaa atc agg gcg cag gtg ggg aat gca atg agc taagaaaaca 977 Leu Glu Ile Arg Ala Gln Val Gly Asn Ala Met Ser         280 285 ctttaaatat tct 990 <210> 72 <211> 289 <212> PRT <213> Corynebacterium glutamicum <400> 72 Met Asp His Ala His Asp Ser Cys Ser Pro Thr Leu Arg Arg Asp Leu   1 5 10 15 Glu Val Thr Gly Gln Leu Gln Pro Glu Lys Ala Val Asp Leu Ala Ala              20 25 30 Pro His Glu Gly Lys Val Ala Asn Ile Thr Lys Val Thr Ser Ser Asn          35 40 45 Met Glu His Thr Ile Thr Gln Ala Ser Lys Ala Lys Glu Val Val Val      50 55 60 Leu Ile Gly His Ser Leu Leu Pro Thr Phe Gln Asp Leu Glu Lys Asp  65 70 75 80 Ile Leu His Phe Gln Ala Gly Asn Lys Gly Arg Phe Ser Val Ala Ile                  85 90 95 Val Asp Pro Asp Arg Ser Ala Asp Val Val Ala Arg Phe Arg Pro Lys             100 105 110 Gln Ile Pro Val Ala Tyr Val Val Lys Asp Gly Ala Ser Ile Ala Glu         115 120 125 Phe Asn Ser Leu Asn Lys Glu Pro Val Ala Gln Trp Leu Asp His Phe     130 135 140 Val Ser Arg Glu Thr Ile Pro Asn Glu Lys Glu Gly Asp Val Asp Lys 145 150 155 160 Gln Ile Asp Pro Arg Leu Trp Arg Ala Ala Glu Leu Val Asn Ala Gly                 165 170 175 Asp Phe Arg Ala Ala Leu Ala Leu Tyr Glu Gln Leu Pro Gln Asp Ala             180 185 190 Thr Val Lys Arg Ala His Ala Ala Val Ser Val Leu Ala Arg Met Ser         195 200 205 Val Ala Asp Arg Gly Glu Asp Pro Ile Glu Lys Ser Arg Arg Asp Pro     210 215 220 Asp Asp Val Asn Lys Ala Leu Ala Ala Ala Asp Met Tyr Val Leu Met 225 230 235 240 Asn Gln Pro Asp Thr Ala Leu Ala His Leu Ala Ala Leu Leu Pro Lys                 245 250 255 Pro Glu Ala Ala Arg Arg Ile Val Glu Leu Leu Asn Leu Phe Asp Pro             260 265 270 Leu Asp Leu Val Ala Leu Glu Ile Arg Ala Gln Val Gly Asn Ala Met         275 280 285 Ser      <210> 73 <211> 900 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (877) <223> RXN01837 <400> 73 cccccatcat tccctcaagg tgtgaagata cggttaggat agaaaagaat ttttttgacg 60 ttggacattc tcaaaatcaa gtagcaaggg atcaaactct gtg agt act aat aag 115                                             Val Ser Thr Asn Lys                                               1 5 gaa cga cgc caa cag gcg ctt tcc cag ctg gag aaa gaa atc aaa agc 163 Glu Arg Arg Gln Gln Ala Leu Ser Gln Leu Glu Lys Glu Ile Lys Ser                  10 15 20 cgg gac cgc aaa gaa aag acc aag cca cta acc gtg gtc ttt gct tcc 211 Arg Asp Arg Lys Glu Lys Thr Lys Pro Leu Thr Val Val Phe Ala Ser              25 30 35 ctg gct gtc atc ctg gtt gtc gtt ggc ggt atc tgg tac gca gct acc 259 Leu Ala Val Ile Leu Val Val Val Gly Gly Ile Trp Tyr Ala Ala Thr          40 45 50 cgc agc acc gaa gac gaa gtc atc acc gct gat gaa aca tcc acc acc 307 Arg Ser Thr Glu Asp Glu Val Ile Thr Ala Asp Glu Thr Ser Thr Thr      55 60 65 gca gag acc cct gac tac cag cca ctg gcg ctg acc cgc acc acc gcg 355 Ala Glu Thr Pro Asp Tyr Gln Pro Leu Ala Leu Thr Arg Thr Thr Ala  70 75 80 85 ctc ggc gac tcc gtg acc tgt gag tac cca gat gct ggc gag gct tcc 403 Leu Gly Asp Ser Val Thr Cys Glu Tyr Pro Asp Ala Gly Glu Ala Ser                  90 95 100 aag gat gtc tcc aag cct gct act gaa aac gtg cca gca acc ggc acc 451 Lys Asp Val Ser Lys Pro Ala Thr Glu Asn Val Pro Ala Thr Gly Thr             105 110 115 gtg acc gtc aac ctg acc acc gcc cag ggc aac atc ggc atg gaa ctt 499 Val Thr Val Asn Leu Thr Thr Ala Gln Gly Asn Ile Gly Met Glu Leu         120 125 130 gat cgc tcc gta tcc cct tgt acc gtc aac gct gtt gag cac atg gct 547 Asp Arg Ser Val Ser Pro Cys Thr Val Asn Ala Val Glu His Met Ala     135 140 145 tcc gag ggc tac tac aac gat act gtc tgc cac cgc atc acc acc tct 595 Ser Glu Gly Tyr Tyr Asn Asp Thr Val Cys His Arg Ile Thr Thr Ser 150 155 160 165 ggc att tac gtt ctc cag tgc ggc gat cca agc agc acc ggc gca ggc 643 Gly Ile Tyr Val Leu Gln Cys Gly Asp Pro Ser Ser Thr Gly Ala Gly                 170 175 180 ggc cca ggg ttc agc ttc gcc aac gaa tac cca acc gac gaa gca act 691 Gly Pro Gly Phe Ser Phe Ala Asn Glu Tyr Pro Thr Asp Glu Ala Thr             185 190 195 gac cta acc acc cca gtc atc tac gag cgc ggc acc atc gcc atg gcc 739 Asp Leu Thr Thr Pro Val Ile Tyr Glu Arg Gly Thr Ile Ala Met Ala         200 205 210 aac gct ggc gct gac acc aac ggg ctc cca gtt ctt cct caa cta cga 787 Asn Ala Gly Ala Asp Thr Asn Gly Leu Pro Val Leu Pro Gln Leu Arg     215 220 225 gga ttc ccc act ggc acc gaa cta cac cta ctt cgg cca gat cac cga 835 Gly Phe Pro Thr Gly Thr Glu Leu His Leu Leu Arg Pro Asp His Arg 230 235 240 245 aga agg cct tgc aac cct cga cgc cat cgc aga agt tgg cac 877 Arg Arg Pro Cys Asn Pro Arg Arg His Arg Arg Ser Trp His                 250 255 tgaaggtgga accggcgacg gag 900 <210> 74 <211> 259 <212> PRT <213> Corynebacterium glutamicum <400> 74 Val Ser Thr Asn Lys Glu Arg Arg Gln Gln Ala Leu Ser Gln Leu Glu   1 5 10 15 Lys Glu Ile Lys Ser Arg Asp Arg Lys Glu Lys Thr Lys Pro Leu Thr              20 25 30 Val Val Phe Ala Ser Leu Ala Val Ile Leu Val Val Val Gly Gly Ile          35 40 45 Trp Tyr Ala Ala Thr Arg Ser Thr Glu Asp Glu Val Ile Thr Ala Asp      50 55 60 Glu Thr Ser Thr Thr Ala Glu Thr Pro Asp Tyr Gln Pro Leu Ala Leu  65 70 75 80 Thr Arg Thr Thr Ala Leu Gly Asp Ser Val Thr Cys Glu Tyr Pro Asp                  85 90 95 Ala Gly Glu Ala Ser Lys Asp Val Ser Lys Pro Ala Thr Glu Asn Val             100 105 110 Pro Ala Thr Gly Thr Val Thr Val Asn Leu Thr Thr Ala Gln Gly Asn         115 120 125 Ile Gly Met Glu Leu Asp Arg Ser Val Ser Pro Cys Thr Val Asn Ala     130 135 140 Val Glu His Met Ala Ser Glu Gly Tyr Tyr Asn Asp Thr Val Cys His 145 150 155 160 Arg Ile Thr Thr Ser Gly Ile Tyr Val Leu Gln Cys Gly Asp Pro Ser                 165 170 175 Ser Thr Gly Ala Gly Gly Pro Gly Phe Ser Phe Ala Asn Glu Tyr Pro             180 185 190 Thr Asp Glu Ala Thr Asp Leu Thr Thr Pro Val Ile Tyr Glu Arg Gly         195 200 205 Thr Ile Ala Met Ala Asn Ala Gly Ala Asp Thr Asn Gly Leu Pro Val     210 215 220 Leu Pro Gln Leu Arg Gly Phe Pro Thr Gly Thr Glu Leu His Leu Leu 225 230 235 240 Arg Pro Asp His Arg Arg Arg Pro Cys Asn Pro Arg Arg His Arg Arg                 245 250 255 Ser Trp His              <210> 75 <211> 741 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (1) .. (741) <223> RXN01926 <400> 75 ctg cga agc ttc tac acc cca gaa caa gcc atc gaa cgc gaa ggc gac 48 Leu Arg Ser Phe Tyr Thr Pro Glu Gln Ala Ile Glu Arg Glu Gly Asp   1 5 10 15 gtc tgg aaa gcc gcc acc gaa gaa gca gaa ctc ctc gca gct gac ggc 96 Val Trp Lys Ala Ala Thr Glu Glu Ala Glu Leu Leu Ala Ala Asp Gly              20 25 30 gcc gtc cac gac cag gaa ctc ttc ctc aac tgc acc acc tcc cca ctg 144 Ala Val His Asp Gln Glu Leu Phe Leu Asn Cys Thr Thr Ser Pro Leu          35 40 45 atc ttc gcc tcc gcg atg ctc aac ttc ggc gtc cac caa atc ctg gac 192 Ile Phe Ala Ser Ala Met Leu Asn Phe Gly Val His Gln Ile Leu Asp      50 55 60 acc ctc tgc caa ctc gca cca tcc ccc gcc ggc cgc gac gca gac ccc 240 Thr Leu Cys Gln Leu Ala Pro Ser Pro Ala Gly Arg Asp Ala Asp Pro  65 70 75 80 aaa gcc ctc gaa gcc gcc acc tcc gca atg gac gac cac cgc gac acc 288 Lys Ala Leu Glu Ala Ala Thr Ser Ala Met Asp Asp His Arg Asp Thr                  85 90 95 acc gac gac ttc tcc ggc gtc gtc ttc aaa gtc caa gcc ggc atg gac 336 Thr Asp Asp Phe Ser Gly Val Val Phe Lys Val Gln Ala Gly Met Asp             100 105 110 aaa aac cac cgc gat acc ctc gcc ttc atg cgc gtc gtc tcc ggc gaa 384 Lys Asn His Arg Asp Thr Leu Ala Phe Met Arg Val Val Ser Gly Glu         115 120 125 ttc gac cgc ggc atg caa gtc acc cac tcc caa tcc ggc cgc agc ttc 432 Phe Asp Arg Gly Met Gln Val Thr His Ser Gln Ser Gly Arg Ser Phe     130 135 140 tcc acc aaa tac gcc ctc acc gtc ttc ggc cgc acc cgc tct acc gtc 480 Ser Thr Lys Tyr Ala Leu Thr Val Phe Gly Arg Thr Arg Ser Thr Val 145 150 155 160 gaa acc gcc ttc ccc ggc gac atc gtc ggc ctc gtc aac gcc ggc gcc 528 Glu Thr Ala Phe Pro Gly Asp Ile Val Gly Leu Val Asn Ala Gly Ala                 165 170 175 ctc gca cca ggc gac acc atc ttc gaa ggc cga aaa atc caa tac cca 576 Leu Ala Pro Gly Asp Thr Ile Phe Glu Gly Arg Lys Ile Gln Tyr Pro             180 185 190 cca atg cca aaa ttc gcg cca gaa cac ttc cgc atc ctg cgc gcc aaa 624 Pro Met Pro Lys Phe Ala Pro Glu His Phe Arg Ile Leu Arg Ala Lys         195 200 205 tca ctc ggc aaa tac aaa cag ttc cgc aaa gcc ctc gag cag ctg gac 672 Ser Leu Gly Lys Tyr Lys Gln Phe Arg Lys Ala Leu Glu Gln Leu Asp     210 215 220 tcc gaa ggt gtc gtc cag atc ctc aag aac gac ctg cgt ggc gac gcc 720 Ser Glu Gly Val Val Gln Ile Leu Lys Asn Asp Leu Arg Gly Asp Ala 225 230 235 240 aac cca ggt cat ggc cgg tgt 741 Asn Pro Gly His Gly Arg Cys                 245 <210> 76 <211> 247 <212> PRT <213> Corynebacterium glutamicum <400> 76 Leu Arg Ser Phe Tyr Thr Pro Glu Gln Ala Ile Glu Arg Glu Gly Asp   1 5 10 15 Val Trp Lys Ala Ala Thr Glu Glu Ala Glu Leu Leu Ala Ala Asp Gly              20 25 30 Ala Val His Asp Gln Glu Leu Phe Leu Asn Cys Thr Thr Ser Pro Leu          35 40 45 Ile Phe Ala Ser Ala Met Leu Asn Phe Gly Val His Gln Ile Leu Asp      50 55 60 Thr Leu Cys Gln Leu Ala Pro Ser Pro Ala Gly Arg Asp Ala Asp Pro  65 70 75 80 Lys Ala Leu Glu Ala Ala Thr Ser Ala Met Asp Asp His Arg Asp Thr                  85 90 95 Thr Asp Asp Phe Ser Gly Val Val Phe Lys Val Gln Ala Gly Met Asp             100 105 110 Lys Asn His Arg Asp Thr Leu Ala Phe Met Arg Val Val Ser Gly Glu         115 120 125 Phe Asp Arg Gly Met Gln Val Thr His Ser Gln Ser Gly Arg Ser Phe     130 135 140 Ser Thr Lys Tyr Ala Leu Thr Val Phe Gly Arg Thr Arg Ser Thr Val 145 150 155 160 Glu Thr Ala Phe Pro Gly Asp Ile Val Gly Leu Val Asn Ala Gly Ala                 165 170 175 Leu Ala Pro Gly Asp Thr Ile Phe Glu Gly Arg Lys Ile Gln Tyr Pro             180 185 190 Pro Met Pro Lys Phe Ala Pro Glu His Phe Arg Ile Leu Arg Ala Lys         195 200 205 Ser Leu Gly Lys Tyr Lys Gln Phe Arg Lys Ala Leu Glu Gln Leu Asp     210 215 220 Ser Glu Gly Val Val Gln Ile Leu Lys Asn Asp Leu Arg Gly Asp Ala 225 230 235 240 Asn Pro Gly His Gly Arg Cys                 245 <210> 77 <211> 478 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101) .. (478) <223> RXN02002 <400> 77 aagtggcaaa aaacgtttca agcaggcaac gccggcgtac aacttcgctg agctggggcg 60 attatggccc agcgcccaca acccgctatt cttaataccc atg agc aac gcc aat 115                                             Met Ser Asn Ala Asn                                               1 5 tcc gac acc acc gcc gcc gag gca cat cgc cgc aga aca ttc gcc gta 163 Ser Asp Thr Thr Ala Ala Glu Ala His Arg Arg Arg Thr Phe Ala Val                  10 15 20 atc gca cac ccc gac gcc ggt aaa tcc acc ctc acc gag gca ttg gcg 211 Ile Ala His Pro Asp Ala Gly Lys Ser Thr Leu Thr Glu Ala Leu Ala              25 30 35 ctg cat gca cac atc atc tcc gaa gcc ggc gcc acc cac ggc aaa gca 259 Leu His Ala His Ile Ile Ser Glu Ala Gly Ala Thr His Gly Lys Ala          40 45 50 ggc cgc aaa gcc acc gtt tcc gac tgg atg gaa atg gaa aaa gac cgc 307 Gly Arg Lys Ala Thr Val Ser Asp Trp Met Glu Met Glu Lys Asp Arg      55 60 65 ggc atc tcc atc gcc tcc tcc gca ctc caa ttc gag tac gca cca gaa 355 Gly Ile Ser Ile Ala Ser Ser Ala Leu Gln Phe Glu Tyr Ala Pro Glu  70 75 80 85 ggc cac gca ggc gag ccc ttc atg atc aac ctc gtg gac acc cca ggc 403 Gly His Ala Gly Glu Pro Phe Met Ile Asn Leu Val Asp Thr Pro Gly                  90 95 100 cac gcc gac ttc tcc gaa gac acc tac cgc gtc ctc atg gcc gtc gac 451 His Ala Asp Phe Ser Glu Asp Thr Tyr Arg Val Leu Met Ala Val Asp             105 110 115 gca gca gtc atg ctt atg cac tcc gtc 478 Ala Ala Val Met Leu Met His Ser Val         120 125 <210> 78 <211> 126 <212> PRT <213> Corynebacterium glutamicum <400> 78 Met Ser Asn Ala Asn Ser Asp Thr Thr Ala Ala Glu Ala His Arg Arg   1 5 10 15 Arg Thr Phe Ala Val Ile Ala His Pro Asp Ala Gly Lys Ser Thr Leu              20 25 30 Thr Glu Ala Leu Ala Leu His Ala His Ile Ile Ser Glu Ala Gly Ala          35 40 45 Thr His Gly Lys Ala Gly Arg Lys Ala Thr Val Ser Asp Trp Met Glu      50 55 60 Met Glu Lys Asp Arg Gly Ile Ser Ile Ala Ser Ser Ala Leu Gln Phe  65 70 75 80 Glu Tyr Ala Pro Glu Gly His Ala Gly Glu Pro Phe Met Ile Asn Leu                  85 90 95 Val Asp Thr Pro Gly His Ala Asp Phe Ser Glu Asp Thr Tyr Arg Val             100 105 110 Leu Met Ala Val Asp Ala Ala Val Met Leu Met His Ser Val         115 120 125 <210> 79 <211> 1080 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1057) <223> RXN02736 <400> 79 cagaggatta cccagcgggt acgtggggtc caaagagcgc tgatgaaatg ctttcccgca 60 acggtcacac ctggcgcagg ccataattta ggggcaaaaa atg atc ttt gaa ctt 115                                             Met Ile Phe Glu Leu                                               1 5 ccg gat acc acc acc cag caa att tcc aag acc cta act cga ctg cgt 163 Pro Asp Thr Thr Thr Gln Gln Ile Ser Lys Thr Leu Thr Arg Leu Arg                  10 15 20 gaa tcg ggc acc cag gtc acc acc ggc cga gtg ctc acc ctc atc gtg 211 Glu Ser Gly Thr Gln Val Thr Thr Gly Arg Val Leu Thr Leu Ile Val              25 30 35 gtc act gac tcc gaa agc gat gtc gct gca gtt acc gag tcc acc aat 259 Val Thr Asp Ser Glu Ser Asp Val Ala Ala Val Thr Glu Ser Thr Asn          40 45 50 gaa gcc tcg cgc gag cac cca tct cgc gtg atc att ttg gtg gtt ggc 307 Glu Ala Ser Arg Glu His Pro Ser Arg Val Ile Ile Leu Val Val Gly      55 60 65 gat aaa act gca gaa aac aaa gtt gac gca gaa gtc cgt atc ggt ggc 355 Asp Lys Thr Ala Glu Asn Lys Val Asp Ala Glu Val Arg Ile Gly Gly  70 75 80 85 gac gct ggt gct tcc gag atg atc atc atg cat ctc aac gga cct gtc 403 Asp Ala Gly Ala Ser Glu Met Ile Met His Leu Asn Gly Pro Val                  90 95 100 gct gac aag ctc cag tat gtc gtc aca cca ctg ttg ctt cct gac acc 451 Ala Asp Lys Leu Gln Tyr Val Val Thr Pro Leu Leu Leu Pro Asp Thr             105 110 115 ccc atc gtt gct tgg tgg cca ggt gaa tca cca aag aat cct tcc cag 499 Pro Ile Val Ala Trp Trp Pro Gly Glu Ser Pro Lys Asn Pro Ser Gln         120 125 130 gac cca att gga cgc atc gca caa cga cgc atc act gat gct ttg tac 547 Asp Pro Ile Gly Arg Ile Ala Gln Arg Arg Ile Thr Asp Ala Leu Tyr     135 140 145 gac cgt gat gac gca cta gaa gat cgt gtt gag aac tat cac cca ggt 595 Asp Arg Asp Asp Ala Leu Glu Asp Arg Val Glu Asn Tyr His Pro Gly 150 155 160 165 gat acc gac atg acg tgg gcg cgc ctt acc cag tgg cgg gga ctt gtt 643 Asp Thr Asp Met Thr Trp Ala Arg Leu Thr Gln Trp Arg Gly Leu Val                 170 175 180 gcc tcc tca ttg gat cac cca cca cac agc gaa atc act tcc gtg agg 691 Ala Ser Ser Leu Asp His Pro Pro His Ser Glu Ile Thr Ser Val Arg             185 190 195 ctg acc ggt gca agc ggc agt acc tcg gtg gat ttg gct gca ggc tgg 739 Leu Thr Gly Ala Ser Gly Ser Thr Ser Val Asp Leu Ala Ala Gly Trp         200 205 210 ttg gcg cgg agg ctg aaa gtg cct gtg atc cgc gag gtg aca gat gct 787 Leu Ala Arg Arg Leu Lys Val Pro Val Ile Arg Glu Val Thr Asp Ala     215 220 225 ccc acc gtg cca acc gat gag ttt ggt act cca ctg ctg gct atc cag 835 Pro Thr Val Pro Thr Asp Glu Phe Gly Thr Pro Leu Leu Ala Ile Gln 230 235 240 245 cgc ctg gag atc gtt cgc acc acc ggc tcg atc atc atc acc atc tat 883 Arg Leu Glu Ile Val Arg Thr Thr Gly Ser Ile Ile Ile Thr Ile Tyr                 250 255 260 gac gct cat acc ctt cag gta gag atg ccg gaa tcc ggc aat gcc cca 931 Asp Ala His Thr Leu Gln Val Glu Met Pro Glu Ser Gly Asn Ala Pro             265 270 275 tcg ctg gtg gct att ggt cgt cga agt gag tcc gac tgc ttg tct gag 979 Ser Leu Val Ala Ile Gly Arg Arg Ser Glu Ser Asp Cys Leu Ser Glu         280 285 290 gag ctt cgc cac atg gat cca gat ttg ggc tac cag cac gca cta tcc 1027 Glu Leu Arg His Met Asp Pro Asp Leu Gly Tyr Gln His Ala Leu Ser     295 300 305 ggc ttg tcc agc gtc aag ctg gaa acc gtc taaggagaaa tacaacacta 1077 Gly Leu Ser Ser Val Lys Leu Glu Thr Val 310 315 tgg 1080 <210> 80 <211> 319 <212> PRT <213> Corynebacterium glutamicum <400> 80 Met Ile Phe Glu Leu Pro Asp Thr Thr Thr Gln Gln Ile Ser Lys Thr   1 5 10 15 Leu Thr Arg Leu Arg Glu Ser Gly Thr Gln Val Thr Thr Gly Arg Val              20 25 30 Leu Thr Leu Ile Val Val Thr Asp Ser Glu Ser Asp Val Ala Ala Val          35 40 45 Thr Glu Ser Thr Asn Glu Ala Ser Arg Glu His Pro Ser Arg Val Ile      50 55 60 Ile Leu Val Val Gly Asp Lys Thr Ala Glu Asn Lys Val Asp Ala Glu  65 70 75 80 Val Arg Ile Gly Gly Asp Ala Gly Ala Ser Glu Met Ile Ile Met His                  85 90 95 Leu Asn Gly Pro Val Ala Asp Lys Leu Gln Tyr Val Val Thr Pro Leu             100 105 110 Leu Leu Pro Asp Thr Pro Ile Val Ala Trp Trp Pro Gly Glu Ser Pro         115 120 125 Lys Asn Pro Ser Gln Asp Pro Ile Gly Arg Ile Ala Gln Arg Arg Ile     130 135 140 Thr Asp Ala Leu Tyr Asp Arg Asp Asp Ala Leu Glu Asp Arg Val Glu 145 150 155 160 Asn Tyr His Pro Gly Asp Thr Asp Met Thr Trp Ala Arg Leu Thr Gln                 165 170 175 Trp Arg Gly Leu Val Ala Ser Ser Leu Asp His Pro Pro His Ser Glu             180 185 190 Ile Thr Ser Val Arg Leu Thr Gly Ala Ser Gly Ser Thr Ser Val Asp         195 200 205 Leu Ala Ala Gly Trp Leu Ala Arg Arg Leu Lys Val Pro Val Ile Arg     210 215 220 Glu Val Thr Asp Ala Pro Thr Val Pro Thr Asp Glu Phe Gly Thr Pro 225 230 235 240 Leu Leu Ala Ile Gln Arg Leu Glu Ile Val Arg Thr Thr Gly Ser Ile                 245 250 255 Ile Ile Thr Ile Tyr Asp Ala His Thr Leu Gln Val Glu Met Pro Glu             260 265 270 Ser Gly Asn Ala Pro Ser Leu Val Ala Ile Gly Arg Arg Ser Glu Ser         275 280 285 Asp Cys Leu Ser Glu Glu Leu Arg His Met Asp Pro Asp Leu Gly Tyr     290 295 300 Gln His Ala Leu Ser Gly Leu Ser Ser Val Lys Leu Glu Thr Val 305 310 315 <210> 81 <211> 331 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101) .. (301) <223> RXS03217 <400> 81 tctgtgaagg tagatggttt gacgaggagt tccaacgact cggacgctgg tgaatcatgc 60 tggcgaacgt agcatcacct gattaggaaa aggtacaaat atg gca cag ggt act 115                                             Met Ala Gln Gly Thr                                               1 5 gtg aaa tgg ttc aac ggc gaa aag gga ttt ggt ttc atc gct ccc aac 163 Val Lys Trp Phe Asn Gly Glu Lys Gly Phe Gly Phe Ile Ala Pro Asn                  10 15 20 gat ggc tcc gca gat ctc ttc gtc cac tac tct gag att cag ggc tcc 211 Asp Gly Ser Ala Asp Leu Phe Val His Tyr Ser Glu Ile Gln Gly Ser              25 30 35 ggt ttc cgt aat ctt gag gaa aac cag cca gtt gaa ttt gag gtc ggc 259 Gly Phe Arg Asn Leu Glu Glu Asn Gln Pro Val Glu Phe Glu Val Gly          40 45 50 gag ggc gcc aag ggc cca cag gct cag cag gtt cgt gct ctc 301 Glu Gly Ala Lys Gly Pro Gln Ala Gln Gln Val Arg Ala Leu      55 60 65 taagctctaa ctgctagcta aaaattccgc 331 <210> 82 <211> 67 <212> PRT <213> Corynebacterium glutamicum <400> 82 Met Ala Gln Gly Thr Val Lys Trp Phe Asn Gly Glu Lys Gly Phe Gly   1 5 10 15 Phe Ile Ala Pro Asn Asp Gly Ser Ala Asp Leu Phe Val His Tyr Ser              20 25 30 Glu Ile Gln Gly Ser Gly Phe Arg Asn Leu Glu Glu Asn Gln Pro Val          35 40 45 Glu Phe Glu Val Gly Glu Gly Ala Lys Gly Pro Gln Ala Gln Gln Val      50 55 60 Arg Ala Leu  65 <210> 83 <211> 324 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101) .. (301) <223> FRXA01917 <400> 83 tctgtgaagg tagatggttt gacgaggagt tccaacgact cggacgctgg tgaatcatgc 60 tggcgaacgt agcatcacct gattaggaaa aggtacaaat atg gca cag ggt act 115                                             Met Ala Gln Gly Thr                                               1 5 gtg aaa tgg ttc aac ggc gaa aag gga ttt ggt ttc atc gct ccc aac 163 Val Lys Trp Phe Asn Gly Glu Lys Gly Phe Gly Phe Ile Ala Pro Asn                  10 15 20 gat ggc tcc gca gat ctc ttc gtc cac tac tct gag att cag ggc tcc 211 Asp Gly Ser Ala Asp Leu Phe Val His Tyr Ser Glu Ile Gln Gly Ser              25 30 35 ggt ttc cgt aat ctt gag gaa aac cag cca gtt gaa ttt gag gtc ggc 259 Gly Phe Arg Asn Leu Glu Glu Asn Gln Pro Val Glu Phe Glu Val Gly          40 45 50 gag ggc gcc aag ggc cca cag gct cag cag gtt cgt gct ctc 301 Glu Gly Ala Lys Gly Pro Gln Ala Gln Gln Val Arg Ala Leu      55 60 65 taagctctaa ctgctagcta aaa 324 <210> 84 <211> 67 <212> PRT <213> Corynebacterium glutamicum <400> 84 Met Ala Gln Gly Thr Val Lys Trp Phe Asn Gly Glu Lys Gly Phe Gly   1 5 10 15 Phe Ile Ala Pro Asn Asp Gly Ser Ala Asp Leu Phe Val His Tyr Ser              20 25 30 Glu Ile Gln Gly Ser Gly Phe Arg Asn Leu Glu Glu Asn Gln Pro Val          35 40 45 Glu Phe Glu Val Gly Glu Gly Ala Lys Gly Pro Gln Ala Gln Gln Val      50 55 60 Arg Ala Leu  65 <210> 85 <211> 504 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (481) <223> RXA02184 <400> 85 tttaccgcga tgcttgcggt gtataataat ttcttctggt caaaaatagt tgatcaattt 60 gaatcagcat atgaattagg aatgaaagtg gtgaggacaa gtg cct gtc gga aca 115                                             Val Pro Val Gly Thr                                               1 5 gtg aag tgg tac gac gcg gag cgt ggt ttc ggc ttt gtc tcc aat cca 163 Val Lys Trp Tyr Asp Ala Glu Arg Gly Phe Gly Phe Val Ser Asn Pro                  10 15 20 ggt ggt gaa gat tgc ttc gta ggt aag caa gta ctt ccc aag gga gtc 211 Gly Gly Glu Asp Cys Phe Val Gly Lys Gln Val Leu Pro Lys Gly Val              25 30 35 acc gaa ttg cac aag gga cag cga atc gat ttt gac ttc gcc gca ggc 259 Thr Glu Leu His Lys Gly Gln Arg Ile Asp Phe Asp Phe Ala Ala Gly          40 45 50 cgt aag ggc cct caa gca ctt cga ata aag att ctt gaa act cca cgc 307 Arg Lys Gly Pro Gln Ala Leu Arg Ile Lys Ile Leu Glu Thr Pro Arg      55 60 65 agg cgt cca cag cac aaa tac aag cca gaa gag ctc aac gga atg atc 355 Arg Arg Pro Gln His Lys Tyr Lys Pro Glu Glu Leu Asn Gly Met Ile  70 75 80 85 tct gac ctc atc acg ctt cta gaa agt gga gtg caa cca ggc ctt gcc 403 Ser Asp Leu Ile Thr Leu Leu Glu Ser Gly Val Gln Pro Gly Leu Ala                  90 95 100 aaa ggg caa tac ccg gag cac aaa gct gga gcg cag gta gca gaa att 451 Lys Gly Gln Tyr Pro Glu His Lys Ala Gly Ala Gln Val Ala Glu Ile             105 110 115 ctt cgc gtt gtt gcg aag gag ctt gag tct taaaacaata aggagaggat 501 Leu Arg Val Val Ala Lys Glu Leu Glu Ser         120 125 ccg 504 <210> 86 <211> 127 <212> PRT <213> Corynebacterium glutamicum <400> 86 Val Pro Val Gly Thr Val Lys Trp Tyr Asp Ala Glu Arg Gly Phe Gly   1 5 10 15 Phe Val Ser Asn Pro Gly Gly Glu Asp Cys Phe Val Gly Lys Gln Val              20 25 30 Leu Pro Lys Gly Val Thr Glu Leu His Lys Gly Gln Arg Ile Asp Phe          35 40 45 Asp Phe Ala Ala Gly Arg Lys Gly Pro Gln Ala Leu Arg Ile Lys Ile      50 55 60 Leu Glu Thr Pro Arg Arg Arg Pro Gln His Lys Tyr Lys Pro Glu Glu  65 70 75 80 Leu Asn Gly Met Ile Ser Asp Leu Ile Thr Leu Leu Glu Ser Gly Val                  85 90 95 Gln Pro Gly Leu Ala Lys Gly Gln Tyr Pro Glu His Lys Ala Gly Ala             100 105 110 Gln Val Ala Glu Ile Leu Arg Val Val Ala Lys Glu Leu Glu Ser         115 120 125 <210> 87 <211> 324 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101) .. (301) <223> RXA00810 <400> 87 tcggcgttcg ttcaagaaac ggccagatgt tgctgttcga gctcatgcaa gagtggaaca 60 tcgaacccgg tagtaattcc aatcagtaaa ggtaagacaa atg gca cag ggc act 115                                             Met Ala Gln Gly Thr                                               1 5 gtt aag tgg ttc aac cca gag aag ggc ttc ggc ttc atc gct cct tcc 163 Val Lys Trp Phe Asn Pro Glu Lys Gly Phe Gly Phe Ile Ala Pro Ser                  10 15 20 gac gga tcc gct gac gtt ttc gtc cac tac tcc gag atc gag ggc aac 211 Asp Gly Ser Ala Asp Val Phe Val His Tyr Ser Glu Ile Glu Gly Asn              25 30 35 ggc ttc cgt acc ctc gag gag aac cag ctc gtc gag ttc gaa atc ggc 259 Gly Phe Arg Thr Leu Glu Glu Asn Gln Leu Val Glu Phe Glu Ile Gly          40 45 50 gag ggc gct aag ggc ctt cag gct cag gct gtt cgt gca atc 301 Glu Gly Ala Lys Gly Leu Gln Ala Gln Ala Val Arg Ala Ile      55 60 65 taattgcatc tgagttcgaa acc 324 <210> 88 <211> 67 <212> PRT <213> Corynebacterium glutamicum <400> 88 Met Ala Gln Gly Thr Val Lys Trp Phe Asn Pro Glu Lys Gly Phe Gly   1 5 10 15 Phe Ile Ala Pro Ser Asp Gly Ser Ala Asp Val Phe Val His Tyr Ser              20 25 30 Glu Ile Glu Gly Asn Gly Phe Arg Thr Leu Glu Glu Asn Gln Leu Val          35 40 45 Glu Phe Glu Ile Gly Glu Gly Ala Lys Gly Leu Gln Ala Gln Ala Val      50 55 60 Arg Ala Ile  65 <210> 89 <211> 1017 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (994) <223> RXA01674 <400> 89 cggcgtcgat tccagaaggt ttgtagacat gcttcaaggt tgcgctaatt gaaaagaacg 60 cggtagacgg tactttcata tccacccata taatgttgat atg gat aat ggg tgg 115                                             Met Asp Asn Gly Trp                                               1 5 ccg aac ctg caa act ctc gca ctc ttt gtg gcg att gtg gaa gag ggg 163 Pro Asn Leu Gln Thr Leu Ala Leu Phe Val Ala Ile Val Glu Glu Gly                  10 15 20 agc ctc ggt gcc ggt gct cga aaa gtc gga atg gcc caa cct aat gcc 211 Ser Leu Gly Ala Gly Ala Arg Lys Val Gly Met Ala Gln Pro Asn Ala              25 30 35 agt cgg gct atc gca gag ctt gag gca gac atg aaa gcc gaa ttg ttg 259 Ser Arg Ala Ile Ala Glu Leu Glu Ala Asp Met Lys Ala Glu Leu Leu          40 45 50 gta cgt cat cct cga gga tca cat cca aca gct gct gga ctt gcg ctt 307 Val Arg His Pro Arg Gly Ser His Pro Thr Ala Ala Gly Leu Ala Leu      55 60 65 gtt gag cat tcg cgc gat ctg ctt caa tct gta caa gaa ttt act gaa 355 Val Glu His Ser Arg Asp Leu Leu Gln Ser Val Gln Glu Phe Thr Glu  70 75 80 85 tgg gtg aca gag gga cga act gag cag ccg ctg aaa ttg cat gtt ggg 403 Trp Val Thr Glu Gly Arg Thr Glu Gln Pro Leu Lys Leu His Val Gly                  90 95 100 gcc agt atg acc att gcc gag gct cta ctt cca gct tgg gtt gcg gac 451 Ala Ser Met Thr Ile Ala Glu Ala Leu Leu Pro Ala Trp Val Ala Asp             105 110 115 atg cgc acg cgt ttt cct gcc tgc cgt gtc gac gtc tct gtg atg aat 499 Met Arg Thr Arg Phe Pro Ala Cys Arg Val Asp Val Ser Val Met Asn         120 125 130 tct tct caa gta att gaa gcc gtc cag aaa ggg cac ttg caa cta ggt 547 Ser Ser Gln Val Ile Glu Ala Val Gln Lys Gly His Leu Gln Leu Gly     135 140 145 ttt att gaa aca ccg cat gtt ccc gta cgg ctt cat gct cgt gtg gtg 595 Phe Ile Glu Thr Pro His Val Pro Val Arg Leu His Ala Arg Val Val 150 155 160 165 caa gag gac aag ctg att gtg gtg att tct cct aat cat gag tgg gct 643 Gln Glu Asp Lys Leu Ile Val Val Ile Ser Pro Asn His Glu Trp Ala                 170 175 180 aat cgc acg ggt agg atc agt ctt cgg gag ttg tcg gaa act ccg ctg 691 Asn Arg Thr Gly Arg Ile Ser Leu Arg Glu Leu Ser Glu Thr Pro Leu             185 190 195 ata gtg agg gaa gtc ggc tca ggt acc cga gaa gca tta caa gaa tta 739 Ile Val Arg Glu Val Gly Ser Gly Thr Arg Glu Ala Leu Gln Glu Leu         200 205 210 ctt gcg gat tat gac atg gct gag ccg att caa gtg tta aac agc aat 787 Leu Ala Asp Tyr Asp Met Ala Glu Pro Ile Gln Val Leu Asn Ser Asn     215 220 225 gct gcg gta cgt gtt gtt gtt gaa gca ggg gca ggt cct gca gta ctt 835 Ala Ala Val Arg Val Val Val Glu Ala Gly Ala Gly Pro Ala Val Leu 230 235 240 245 ggt gaa tta gcc ttg cgt gat cat ctt gcg ctc ggc agg ctg ttg agt 883 Gly Glu Leu Ala Leu Arg Asp His Leu Ala Leu Gly Arg Leu Leu Ser                 250 255 260 gtg cca ttt gaa ggc agt gga gtt act cgt cct ctt aca gct gtg tgg 931 Val Pro Phe Glu Gly Ser Gly Val Thr Arg Pro Leu Thr Ala Val Trp             265 270 275 agt gga ccc cgc aga ttg ccg att cta gcg gga gaa tta gtg tcc atc 979 Ser Gly Pro Arg Arg Leu Pro Ile Leu Ala Gly Glu Leu Val Ser Ile         280 285 290 gca tcg aac cac atc tgattttgag ccctggctaa cgg 1017 Ala Ser Asn His Ile     295 <210> 90 <211> 298 <212> PRT <213> Corynebacterium glutamicum <400> 90 Met Asp Asn Gly Trp Pro Asn Leu Gln Thr Leu Ala Leu Phe Val Ala   1 5 10 15 Ile Val Glu Glu Gly Ser Leu Gly Ala Gly Ala Arg Lys Val Gly Met              20 25 30 Ala Gln Pro Asn Ala Ser Arg Ala Ile Ala Glu Leu Glu Ala Asp Met          35 40 45 Lys Ala Glu Leu Leu Val Arg His Pro Arg Gly Ser His Pro Thr Ala      50 55 60 Ala Gly Leu Ala Leu Val Glu His Ser Arg Asp Leu Leu Gln Ser Val  65 70 75 80 Gln Glu Phe Thr Glu Trp Val Thr Glu Gly Arg Thr Glu Gln Pro Leu                  85 90 95 Lys Leu His Val Gly Ala Ser Met Thr Ile Ala Glu Ala Leu Leu Pro             100 105 110 Ala Trp Val Ala Asp Met Arg Thr Arg Phe Pro Ala Cys Arg Val Asp         115 120 125 Val Ser Val Met Asn Ser Ser Gln Val Ile Glu Ala Val Gln Lys Gly     130 135 140 His Leu Gln Leu Gly Phe Ile Glu Thr Pro His Val Pro Val Arg Leu 145 150 155 160 His Ala Arg Val Val Gln Glu Asp Lys Leu Ile Val Val Ile Ser Pro                 165 170 175 Asn His Glu Trp Ala Asn Arg Thr Gly Arg Ile Ser Leu Arg Glu Leu             180 185 190 Ser Glu Thr Pro Leu Ile Val Arg Glu Val Gly Ser Gly Thr Arg Glu         195 200 205 Ala Leu Gln Glu Leu Leu Ala Asp Tyr Asp Met Ala Glu Pro Ile Gln     210 215 220 Val Leu Asn Ser Asn Ala Ala Val Arg Val Val Val Glu Ala Gly Ala 225 230 235 240 Gly Pro Ala Val Leu Gly Glu Leu Ala Leu Arg Asp His Leu Ala Leu                 245 250 255 Gly Arg Leu Leu Ser Val Pro Phe Glu Gly Ser Gly Val Thr Arg Pro             260 265 270 Leu Thr Ala Val Trp Ser Gly Pro Arg Arg Leu Pro Ile Leu Ala Gly         275 280 285 Glu Leu Val Ser Ile Ala Ser Asn His Ile     290 295 <210> 91 <211> 1214 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (1) .. (1191) <223> RXA02431 <400> 91 gtg gtg gtg aca ccc cgt cat atc gtt tac tcc gca gcc tcg cgc cgg 48 Val Val Val Thr Pro Arg His Ile Val Tyr Ser Ala Ala Ser Arg Arg   1 5 10 15 gtg ttc caa atc gtg gaa aaa cgc gcc gga att gtc gaa cgc ctc agc 96 Val Phe Gln Ile Val Glu Lys Arg Ala Gly Ile Val Glu Arg Leu Ser              20 25 30 atc gat gaa ggc ttc atg gaa cca gag gct ctc gtt gga gcc acc cca 144 Ile Asp Glu Gly Phe Met Glu Pro Glu Ala Leu Val Gly Ala Thr Pro          35 40 45 gaa gag gtg aaa cag tgg gcg gaa gaa tta cgc gcg gaa att aaa gaa 192 Glu Glu Val Lys Gln Trp Ala Glu Glu Leu Arg Ala Glu Ile Lys Glu      50 55 60 gtt act ggc tta ccc tcc tcg gtt ggt gct ggc tcc ggt aag cag atc 240 Val Thr Gly Leu Pro Ser Ser Val Gly Ala Gly Ser Gly Lys Gln Ile  65 70 75 80 gcc aaa att ggt tca ggc gaa gca aag cca gat ggt gtg ttt gtc gtg 288 Ala Lys Ile Gly Ser Gly Glu Ala Lys Pro Asp Gly Val Phe Val Val                  85 90 95 cca gta gac aag caa cat gac ttg ctt gat cca ctt cct gtg ggc gca 336 Pro Val Asp Lys Gln His Asp Leu Leu Asp Pro Leu Pro Val Gly Ala             100 105 110 ctt tgg gga gtg ggt cct gtg aca ggc tcc aag ctt gcc tca atg ggg 384 Leu Trp Gly Val Gly Pro Val Thr Gly Ser Lys Leu Ala Ser Met Gly         115 120 125 gtg gaa aca att ggt gat cta gca gcg cta acc caa aaa gaa gta gaa 432 Val Glu Thr Ile Gly Asp Leu Ala Ala Leu Thr Gln Lys Glu Val Glu     130 135 140 atc agc ctc ggt gca acc atc gga ata tca ctg tgg aac ctt gcc cga 480 Ile Ser Leu Gly Ala Thr Ile Gly Ile Ser Leu Trp Asn Leu Ala Arg 145 150 155 160 gga atc gac gac cgc cct gtg gaa ccc cgc gcc gaa gca aaa cag atc 528 Gly Ile Asp Asp Arg Pro Val Glu Pro Arg Ala Glu Ala Lys Gln Ile                 165 170 175 tcc caa gag cac acc tat gaa aaa gac ctc ctc acc agg caa caa gta 576 Ser Gln Glu His Thr Tyr Glu Lys Asp Leu Leu Thr Arg Gln Gln Val             180 185 190 gat gct gcc atc att cga tca gcc gaa ggc gca cac cga cgg ctc ctc 624 Asp Ala Ala Ile Ile Arg Ser Ala Glu Gly Ala His Arg Arg Leu Leu         195 200 205 aaa gac gga cgc ggt gcc aga act gtc agc gtg aaa ctg cgg atg gcc 672 Lys Asp Gly Arg Gly Ala Arg Thr Val Ser Val Lys Leu Arg Met Ala     210 215 220 gac ttt cgt att gag tct cgt tcc tac acc ttg tcc tat gcc acc gat 720 Asp Phe Arg Ile Glu Ser Arg Ser Tyr Thr Leu Ser Tyr Ala Thr Asp 225 230 235 240 gat tac gca act ctt gag gca aca gca ttc cga ctt gcc cgc tac ccc 768 Asp Tyr Ala Thr Leu Glu Ala Thr Ala Phe Arg Leu Ala Arg Tyr Pro                 245 250 255 gga gaa gta ggc ccc atc cgc ctt gtc gga gta agt ttt tct ggt ttg 816 Gly Glu Val Gly Pro Ile Arg Leu Val Gly Val Ser Phe Ser Gly Leu             260 265 270 gaa gaa tcc cgc caa gac atc ctc ttc ccg gaa ctt gac caa caa atc 864 Glu Glu Ser Arg Gln Asp Ile Leu Phe Pro Glu Leu Asp Gln Gln Ile         275 280 285 atc gta cca cca gca ccc gac acc gat tat gag gta ggc gtg caa tcc 912 Ile Val Pro Pro Ala Pro Asp Thr Asp Tyr Glu Val Gly Val Gln Ser     290 295 300 tct tct agt tcc gaa agt act caa gtt gaa gcg ccg caa gat gtc gcg 960 Ser Ser Ser Ser Glu Ser Thr Gln Val Glu Ala Pro Gln Asp Val Ala 305 310 315 320 ttg agt atg tgg tgc gca acg caa gat gtc tac cac cca gaa tat ggc 1008 Leu Ser Met Trp Cys Ala Thr Gln Asp Val Tyr His Pro Glu Tyr Gly                 325 330 335 cac ggt tgg gta caa ggt gcc ggt cac ggt gtt gta tca gta cgt ttt 1056 His Gly Trp Val Gln Gly Ala Gly His Gly Val Val Ser Val Arg Phe             340 345 350 gaa acc cgc agc acc aca aaa ggg cga act aaa agt ttt tcc atg gat 1104 Glu Thr Arg Ser Thr Thr Lys Gly Arg Thr Lys Ser Phe Ser Met Asp         355 360 365 gac ccg gac ctc acc ccg gca gac cct cta gat agt ttg gat tgg gct 1152 Asp Pro Asp Leu Thr Pro Ala Asp Pro Leu Asp Ser Leu Asp Trp Ala     370 375 380 gac tgg ttt gct gaa aat ggt gaa acg ggg gat gac gaa tagggtttca 1201 Asp Trp Phe Ala Glu Asn Gly Glu Thr Gly Asp Asp Glu 385 390 395 tcgggtttcg ggg 1214 <210> 92 <211> 397 <212> PRT <213> Corynebacterium glutamicum <400> 92 Val Val Val Thr Pro Arg His Ile Val Tyr Ser Ala Ala Ser Arg Arg   1 5 10 15 Val Phe Gln Ile Val Glu Lys Arg Ala Gly Ile Val Glu Arg Leu Ser              20 25 30 Ile Asp Glu Gly Phe Met Glu Pro Glu Ala Leu Val Gly Ala Thr Pro          35 40 45 Glu Glu Val Lys Gln Trp Ala Glu Glu Leu Arg Ala Glu Ile Lys Glu      50 55 60 Val Thr Gly Leu Pro Ser Ser Val Gly Ala Gly Ser Gly Lys Gln Ile  65 70 75 80 Ala Lys Ile Gly Ser Gly Glu Ala Lys Pro Asp Gly Val Phe Val Val                  85 90 95 Pro Val Asp Lys Gln His Asp Leu Leu Asp Pro Leu Pro Val Gly Ala             100 105 110 Leu Trp Gly Val Gly Pro Val Thr Gly Ser Lys Leu Ala Ser Met Gly         115 120 125 Val Glu Thr Ile Gly Asp Leu Ala Ala Leu Thr Gln Lys Glu Val Glu     130 135 140 Ile Ser Leu Gly Ala Thr Ile Gly Ile Ser Leu Trp Asn Leu Ala Arg 145 150 155 160 Gly Ile Asp Asp Arg Pro Val Glu Pro Arg Ala Glu Ala Lys Gln Ile                 165 170 175 Ser Gln Glu His Thr Tyr Glu Lys Asp Leu Leu Thr Arg Gln Gln Val             180 185 190 Asp Ala Ala Ile Ile Arg Ser Ala Glu Gly Ala His Arg Arg Leu Leu         195 200 205 Lys Asp Gly Arg Gly Ala Arg Thr Val Ser Val Lys Leu Arg Met Ala     210 215 220 Asp Phe Arg Ile Glu Ser Arg Ser Tyr Thr Leu Ser Tyr Ala Thr Asp 225 230 235 240 Asp Tyr Ala Thr Leu Glu Ala Thr Ala Phe Arg Leu Ala Arg Tyr Pro                 245 250 255 Gly Glu Val Gly Pro Ile Arg Leu Val Gly Val Ser Phe Ser Gly Leu             260 265 270 Glu Glu Ser Arg Gln Asp Ile Leu Phe Pro Glu Leu Asp Gln Gln Ile         275 280 285 Ile Val Pro Pro Ala Pro Asp Thr Asp Tyr Glu Val Gly Val Gln Ser     290 295 300 Ser Ser Ser Ser Glu Ser Thr Gln Val Glu Ala Pro Gln Asp Val Ala 305 310 315 320 Leu Ser Met Trp Cys Ala Thr Gln Asp Val Tyr His Pro Glu Tyr Gly                 325 330 335 His Gly Trp Val Gln Gly Ala Gly His Gly Val Val Ser Val Arg Phe             340 345 350 Glu Thr Arg Ser Thr Thr Lys Gly Arg Thr Lys Ser Phe Ser Met Asp         355 360 365 Asp Pro Asp Leu Thr Pro Ala Asp Pro Leu Asp Ser Leu Asp Trp Ala     370 375 380 Asp Trp Phe Ala Glu Asn Gly Glu Thr Gly Asp Asp Glu 385 390 395 <210> 93 <211> 558 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (535) <223> RXA02446 <400> 93 caggaaatct tgctgtctaa tgcacaacgg aaaactgctc acggacgaac tagttgtagc 60 gagcgtattc tttgtgttct ctcacgacag gaatactgct atg gcg atc gag tcc 115                                             Met Ala Ile Glu Ser                                               1 5 atc gcg tac acc agt gaa gca ctc tca acc ggc agt ggc cgg ctg ggg 163 Ile Ala Tyr Thr Ser Glu Ala Leu Ser Thr Gly Ser Gly Arg Leu Gly                  10 15 20 cat gtg cgc tcc aca gat ggt gcg ctc gaa ttt gaa atg aca ccg cca 211 His Val Arg Ser Thr Asp Gly Ala Leu Glu Phe Glu Met Thr Pro Pro              25 30 35 aag gct ttg ggc gga tcc ggt gaa ggc acc aat cca gaa cag ctg ttc 259 Lys Ala Leu Gly Gly Ser Gly Glu Gly Thr Asn Pro Glu Gln Leu Phe          40 45 50 gcg gta ggt tac gca gcc tgt ttc cac tct gcc atg cac tct gtc gca 307 Ala Val Gly Tyr Ala Ala Cys Phe His Ser Ala Met His Ser Val Ala      55 60 65 cgc agc cgc aag atc act ctt gaa gac aca gcg gtt ggt gcc cga gtt 355 Arg Ser Arg Lys Ile Thr Leu Glu Asp Thr Ala Val Gly Ala Arg Val  70 75 80 85 agc atc ggg cca aac ggc gct ggt gga ttt gag att gcc gta gaa ctc 403 Ser Ile Gly Pro Asn Gly Ala Gly Gly Phe Glu Ile Ala Val Glu Leu                  90 95 100 gaa gta tcg att cct caa ttg cca caa gca gaa gcc cag gaa ctt gct 451 Glu Val Ser Ile Pro Gln Leu Pro Gln Ala Glu Ala Gln Glu Leu Ala             105 110 115 gat gcc gcg cac cag gtg tgc ccg tat tcc aac gcc aca cga ggc aat 499 Asp Ala Ala His Gln Val Cys Pro Tyr Ser Asn Ala Thr Arg Gly Asn         120 125 130 atc tcg gta act gtg tca gtc atc gac gaa gag gct taaaaccaca 545 Ile Ser Val Thr Val Ser Val Ile Asp Glu Glu Ala     135 140 145 ggattaacaa aac 558 <210> 94 <211> 145 <212> PRT <213> Corynebacterium glutamicum <400> 94 Met Ala Ile Glu Ser Ile Ala Tyr Thr Ser Glu Ala Leu Ser Thr Gly   1 5 10 15 Ser Gly Arg Leu Gly His Val Arg Ser Thr Asp Gly Ala Leu Glu Phe              20 25 30 Glu Met Thr Pro Pro Lys Ala Leu Gly Gly Ser Gly Glu Gly Thr Asn          35 40 45 Pro Glu Gln Leu Phe Ala Val Gly Tyr Ala Ala Cys Phe His Ser Ala      50 55 60 Met His Ser Val Ala Arg Ser Arg Lys Ile Thr Leu Glu Asp Thr Ala  65 70 75 80 Val Gly Ala Arg Val Ser Ile Gly Pro Asn Gly Ala Gly Gly Phe Glu                  85 90 95 Ile Ala Val Glu Leu Glu Val Ser Ile Pro Gln Leu Pro Gln Ala Glu             100 105 110 Ala Gln Glu Leu Ala Asp Ala Ala His Gln Val Cys Pro Tyr Ser Asn         115 120 125 Ala Thr Arg Gly Asn Ile Ser Val Thr Val Ser Val Ile Asp Glu Glu     130 135 140 Ala 145 <210> 95 <211> 1206 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (1183) <223> RXA02861 <400> 95 ccctcgatta ttacaatccg gctacttcgg aagcgtttgt ggccggattt ttctatcctg 60 gaaggtatta atacttcttt aagggtcgga ggattttcgt atg tct act aga aca 115                                             Met Ser Thr Arg Thr                                               1 5 acg cca caa gac cgt tat acc gac gaa tac ggc atc gaa cgc gtc aac 163 Thr Pro Gln Asp Arg Tyr Thr Asp Glu Tyr Gly Ile Glu Arg Val Asn                  10 15 20 aag gat gaa ccc ggc ctg gtg gac aaa ctc cgg gac aag cac gac tgg 211 Lys Asp Glu Pro Gly Leu Val Asp Lys Leu Arg Asp Lys His Asp Trp              25 30 35 ttt gat cat ctc atg cgc atg aat gaa cgt ttc ggc gca aaa ggt ggc 259 Phe Asp His Leu Met Arg Met Asn Glu Arg Phe Gly Ala Lys Gly Gly          40 45 50 aac caa ttg tcg gcg ggt att acg tat ttc tcc gtg ctg tcg atc ttc 307 Asn Gln Leu Ser Ala Gly Ile Thr Tyr Phe Ser Val Leu Ser Ile Phe      55 60 65 ccg att gcc atg ctt gtc ttc ggt att gca ggt gtc atc ctt gcc gga 355 Pro Ile Ala Met Leu Val Phe Gly Ile Ala Gly Val Ile Leu Ala Gly  70 75 80 85 aac cct gaa gtt ctc aca gat att caa aat cga atc aac gat gct tta 403 Asn Pro Glu Val Leu Thr Asp Ile Gln Asn Arg Ile Asn Asp Ala Leu                  90 95 100 gaa ggc gag atc ggt aac acc gtc aac ggc atc att gat tcc gcg att 451 Glu Gly Glu Ile Gly Asn Thr Val Asn Gly Ile Ile Asp Ser Ala Ile             105 110 115 gcg cag cgt ggt gct gtg ttg ggc att ggt ggt gta act gcc ctg tgg 499 Ala Gln Arg Gly Ala Val Leu Gly Ile Gly Gly Val Thr Ala Leu Trp         120 125 130 tct gga ctg ggg tgg atg gcg aac ctg cgc ttt gga gtt tcc cgc atg 547 Ser Gly Leu Gly Trp Met Ala Asn Leu Arg Phe Gly Val Ser Arg Met     135 140 145 tgg gcc att gac cca act gaa ggc aac ttc att caa aag aag ctc acc 595 Trp Ala Ile Asp Pro Thr Glu Gly Asn Phe Ile Gln Lys Lys Leu Thr 150 155 160 165 gac ttg gtc gcg ctg atc gtc ttg ctg ctg gcc atg ggc gta gcc ttc 643 Asp Leu Val Ala Leu Ile Val Leu Leu Leu Ala Met Gly Val Ala Phe                 170 175 180 ggt atc acg gcg ctc ggt gct tcc gga cta acc aaa aac ctg ctg gac 691 Gly Ile Thr Ala Leu Gly Ala Ser Gly Leu Thr Lys Asn Leu Leu Asp             185 190 195 ttt gtg ggc ctg ggg gag att ccg ggc att agc tac atc acc tgg gtg 739 Phe Val Gly Leu Gly Glu Ile Pro Gly Ile Ser Tyr Ile Thr Trp Val         200 205 210 gtc gca gca ctt gtt ggt gtc ttg gct aac ttc ctg gtg ttc atg tgg 787 Val Ala Ala Leu Val Gly Val Leu Ala Asn Phe Leu Val Phe Met Trp     215 220 225 ctg att ttc tcc ctg cca cgt acc aaa gtt ccc atg aaa ccg ggt ctt 835 Leu Ile Phe Ser Leu Pro Arg Thr Lys Val Pro Met Lys Pro Gly Leu 230 235 240 245 cag gca gca ctg ctt ggc gca atc ggt ttt gag gtg gtc aag cag gtt 883 Gln Ala Ala Leu Leu Gly Ala Ile Gly Phe Glu Val Val Lys Gln Val                 250 255 260 gga tcg ctg ttg gct tca aat gca ttg agt aac ccc gcg ggt gca gca 931 Gly Ser Leu Leu Ala Ser Asn Ala Leu Ser Asn Pro Ala Gly Ala Ala             265 270 275 ttc ggt ccg atc atc ggc atc atg gtt gtg ctg tat ttg atc tgg cgc 979 Phe Gly Pro Ile Ile Gly Ile Met Val Val Leu Tyr Leu Ile Trp Arg         280 285 290 atc ctc atg tat tgc tct gcg tgg gct gcc acc agt gaa gaa gcg ttg 1027 Ile Leu Met Tyr Cys Ser Ala Trp Ala Ala Thr Ser Glu Glu Ala Leu     295 300 305 cgt ctt gcg act gtt cca gca cca gag cct gcg atc att cgg gtt cgc 1075 Arg Leu Ala Thr Val Pro Ala Pro Glu Pro Ala Ile Ile Arg Val Arg 310 315 320 325 cat gaa att gat cca ggt gaa gaa gtc tcc caa tct gct cga aaa gtg 1123 His Glu Ile Asp Pro Gly Glu Glu Val Ser Gln Ser Ala Arg Lys Val                 330 335 340 ggc att gga gtg gcc gtg ggt gcc gcg act gcg ggt gct ttt gcg ctg 1171 Gly Ile Gly Val Ala Val Gly Ala Ala Thr Ala Gly Ala Phe Ala Leu             345 350 355 ttg cgt aaa aaa tagtttttat taagggcatt ccc 1206 Leu Arg Lys Lys         360 <210> 96 <211> 361 <212> PRT <213> Corynebacterium glutamicum <400> 96 Met Ser Thr Arg Thr Thr Pro Gln Asp Arg Tyr Thr Asp Glu Tyr Gly   1 5 10 15 Ile Glu Arg Val Asn Lys Asp Glu Pro Gly Leu Val Asp Lys Leu Arg              20 25 30 Asp Lys His Asp Trp Phe Asp His Leu Met Arg Met Asn Glu Arg Phe          35 40 45 Gly Ala Lys Gly Gly Asn Gln Leu Ser Ala Gly Ile Thr Tyr Phe Ser      50 55 60 Val Leu Ser Ile Phe Pro Ile Ala Met Leu Val Phe Gly Ile Ala Gly  65 70 75 80 Val Ile Leu Ala Gly Asn Pro Glu Val Leu Thr Asp Ile Gln Asn Arg                  85 90 95 Ile Asn Asp Ala Leu Glu Gly Glu Ile Gly Asn Thr Val Asn Gly Ile             100 105 110 Ile Asp Ser Ala Ile Ala Gln Arg Gly Ala Val Leu Gly Ile Gly Gly         115 120 125 Val Thr Ala Leu Trp Ser Gly Leu Gly Trp Met Ala Asn Leu Arg Phe     130 135 140 Gly Val Ser Arg Met Trp Ala Ile Asp Pro Thr Glu Gly Asn Phe Ile 145 150 155 160 Gln Lys Lys Leu Thr Asp Leu Val Ala Leu Ile Val Leu Leu Leu Ala                 165 170 175 Met Gly Val Ala Phe Gly Ile Thr Ala Leu Gly Ala Ser Gly Leu Thr             180 185 190 Lys Asn Leu Leu Asp Phe Val Gly Leu Gly Glu Ile Pro Gly Ile Ser         195 200 205 Tyr Ile Thr Trp Val Val Ala Ala Leu Val Gly Val Leu Ala Asn Phe     210 215 220 Leu Val Phe Met Trp Leu Ile Phe Ser Leu Pro Arg Thr Lys Val Pro 225 230 235 240 Met Lys Pro Gly Leu Gln Ala Ala Leu Leu Gly Ala Ile Gly Phe Glu                 245 250 255 Val Val Lys Gln Val Gly Ser Leu Leu Ala Ser Asn Ala Leu Ser Asn             260 265 270 Pro Ala Gly Ala Ala Phe Gly Pro Ile Ile Gly Ile Met Val Val Leu         275 280 285 Tyr Leu Ile Trp Arg Ile Leu Met Tyr Cys Ser Ala Trp Ala Ala Thr     290 295 300 Ser Glu Glu Ala Leu Arg Leu Ala Thr Val Pro Ala Pro Glu Pro Ala 305 310 315 320 Ile Ile Arg Val Arg His Glu Ile Asp Pro Gly Glu Glu Val Ser Gln                 325 330 335 Ser Ala Arg Lys Val Gly Ile Gly Val Ala Val Gly Ala Ala Thr Ala             340 345 350 Gly Ala Phe Ala Leu Leu Arg Lys Lys         355 360 <210> 97 <211> 753 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101) .. (730) <223> RXA00981 <400> 97 aaccaatggc tgggtactga tgtggtgatc agtgcccagt ttcttctttc tactagtgtc 60 ggatagaagt acccccagtc cagaatgaag gtcaccacca atg tca gag aat ttg 115                                             Met ser glu asn leu                                               1 5 cca gcg ccc gag aat ctc ctg gac gcc gag aga att cag atg atc aag 163 Pro Ala Pro Glu Asn Leu Leu Asp Ala Glu Arg Ile Gln Met Ile Lys                  10 15 20 aac ttc cgc aac gaa tta acg ggg ttc atg ctc aac tac caa ttt ggc 211 Asn Phe Arg Asn Glu Leu Thr Gly Phe Met Leu Asn Tyr Gln Phe Gly              25 30 35 att gat gag atc ctg acc aag atc aac atc ctg aaa act gaa ttc agc 259 Ile Asp Glu Ile Leu Thr Lys Ile Asn Ile Leu Lys Thr Glu Phe Ser          40 45 50 cag ctg cac gaa tac gca cct atc gag cac gta tct tca cga ttg aag 307 Gln Leu His Glu Tyr Ala Pro Ile Glu His Val Ser Ser Arg Leu Lys      55 60 65 aca cca gaa agc atc gtc aaa aag gtc atc cga aaa gga gac gag ctc 355 Thr Pro Glu Ser Ile Val Lys Lys Val Ile Arg Lys Gly Asp Glu Leu  70 75 80 85 tcc ctc gca gct atc aaa gac aca gtg ttt gat atc gca ggc att cga 403 Ser Leu Ala Ala Ile Lys Asp Thr Val Phe Asp Ile Ala Gly Ile Arg                  90 95 100 atc gtc tgc agt ttc ctc aaa gat gcc tac gca atc gcc gat atg ctg 451 Ile Val Cys Ser Phe Leu Lys Asp Ala Tyr Ala Ile Ala Asp Met Leu             105 110 115 acc aac caa aaa gac gtc acg gtc atc gag gcc aaa gac tac atc gct 499 Thr Asn Gln Lys Asp Val Thr Val Ile Glu Ala Lys Asp Tyr Ile Ala         120 125 130 aac cca aag ccg aac ggc tac aag agt ttg cac ctt atc ctc caa gtg 547 Asn Pro Lys Pro Asn Gly Tyr Lys Ser Leu His Leu Ile Leu Gln Val     135 140 145 cct gtc ttc ctg tct aac tcc gtg gaa aag gtc aat gtt gaa gtc cag 595 Pro Val Phe Leu Ser Asn Ser Val Glu Lys Val Asn Val Glu Val Gln 150 155 160 165 atc cgc acc att gcc atg gac ttc tgg gca agc ctc gag cac aaa atc 643 Ile Arg Thr Ile Ala Met Asp Phe Trp Ala Ser Leu Glu His Lys Ile                 170 175 180 tac tac aaa ttt gaa caa gaa gtt cct cag tca atc ctt gat gag ctc 691 Tyr Tyr Lys Phe Glu Gln Glu Val Pro Gln Ser Ile Leu Asp Glu Leu             185 190 195 agt gaa gat gga aag aat cca cgg gga agt gaa gtc act taaacctcca 740 Ser Glu Asp Gly Lys Asn Pro Arg Gly Ser Glu Val Thr         200 205 210 gttgaaacca ctg 753 <210> 98 <211> 210 <212> PRT <213> Corynebacterium glutamicum <400> 98 Met Ser Glu Asn Leu Pro Ala Pro Glu Asn Leu Leu Asp Ala Glu Arg   1 5 10 15 Ile Gln Met Ile Lys Asn Phe Arg Asn Glu Leu Thr Gly Phe Met Leu              20 25 30 Asn Tyr Gln Phe Gly Ile Asp Glu Ile Leu Thr Lys Ile Asn Ile Leu          35 40 45 Lys Thr Glu Phe Ser Gln Leu His Glu Tyr Ala Pro Ile Glu His Val      50 55 60 Ser Ser Arg Leu Lys Thr Pro Glu Ser Ile Val Lys Lys Val Ile Arg  65 70 75 80 Lys Gly Asp Glu Leu Ser Leu Ala Ala Ile Lys Asp Thr Val Phe Asp                  85 90 95 Ile Ala Gly Ile Arg Ile Val Cys Ser Phe Leu Lys Asp Ala Tyr Ala             100 105 110 Ile Ala Asp Met Leu Thr Asn Gln Lys Asp Val Thr Val Ile Glu Ala         115 120 125 Lys Asp Tyr Ile Ala Asn Pro Lys Pro Asn Gly Tyr Lys Ser Leu His     130 135 140 Leu Ile Leu Gln Val Pro Val Phe Leu Ser Asn Ser Val Glu Lys Val 145 150 155 160 Asn Val Glu Val Gln Ile Arg Thr Ile Ala Met Asp Phe Trp Ala Ser                 165 170 175 Leu Glu His Lys Ile Tyr Tyr Lys Phe Glu Gln Glu Val Pro Gln Ser             180 185 190 Ile Leu Asp Glu Leu Ser Glu Asp Gly Lys Asn Pro Arg Gly Ser Glu         195 200 205 Val Thr     210 <210> 99 <211> 1098 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101) .. (1075) <223> RXN00786 <400> 99 tccttcttcc accagctaaa agtgaattac cccgcctttg tcggggtggt tgcattccca 60 gtctaggtgt ttagcctcaa cgttggatac gctgggaggc atg agc tca cct gtt 115                                             Met ser ser pro val                                               1 5 atc agc ccc gaa acc aaa acc gga aag aag atc ctg ctt gca gcc cct 163 Ile Ser Pro Glu Thr Lys Thr Gly Lys Lys Ile Leu Leu Ala Ala Pro                  10 15 20 cgc gga tac tgt gcc ggc gta gac cgt gca gtg gaa acc gtc gag cgc 211 Arg Gly Tyr Cys Ala Gly Val Asp Arg Ala Val Glu Thr Val Glu Arg              25 30 35 gcg ctc gag gaa tac ggc gcc cca att tat gtc cgt aaa gaa atc gtg 259 Ala Leu Glu Glu Tyr Gly Ala Pro Ile Tyr Val Arg Lys Glu Ile Val          40 45 50 cac aac cgt tac gtt gtg gac acc ctg gca gaa aag ggc gcg att ttt 307 His Asn Arg Tyr Val Val Asp Thr Leu Ala Glu Lys Gly Ala Ile Phe      55 60 65 gtc aac gaa gca tct gaa gca cca gaa ggt gcc aac atg gtg ttc tct 355 Val Asn Glu Ala Ser Glu Ala Pro Glu Gly Ala Asn Met Val Phe Ser  70 75 80 85 gca cac ggc gtg agc cca atg gtc cac gaa gaa gct gca gct aaa aac 403 Ala His Gly Val Ser Pro Met Val His Glu Glu Ala Ala Ala Lys Asn                  90 95 100 atc aag gct att gac gcg gcc tgc ccg ctg gtc acc aaa gtg cac aag 451 Ile Lys Ala Ile Asp Ala Ala Cys Pro Leu Val Thr Lys Val His Lys             105 110 115 gaa gtc cag cgc ttt gat aag cag gga ttc cac att ctc ttc atc ggt 499 Glu Val Gln Arg Phe Asp Lys Gln Gly Phe His Ile Leu Phe Ile Gly         120 125 130 cac gaa ggc cat gaa gaa gta gag ggc acc atg ggt cat tcc gtt gag 547 His Glu Gly His Glu Glu Val Glu Gly Thr Met Gly His Ser Val Glu     135 140 145 aaa acc cac ctg gtt gac ggc gtt gct ggc att gcc acc ctg cct gaa 595 Lys Thr His Leu Val Asp Gly Val Ala Gly Ile Ala Thr Leu Pro Glu 150 155 160 165 ttc tta aac gat gaa cca aac ctg atc tgg ctg tct cag acc acg ctt 643 Phe Leu Asn Asp Glu Pro Asn Leu Ile Trp Leu Ser Gln Thr Thr Leu                 170 175 180 tct gtg gac gag acc atg gag atc gtc cgc gag ctg aag gtg aag ttc 691 Ser Val Asp Glu Thr Met Glu Ile Val Arg Glu Leu Lys Val Lys Phe             185 190 195 cct cag ctg cag gat cca ccg tca gat gat att tgc tac gcc acg cag 739 Pro Gln Leu Gln Asp Pro Pro Ser Asp Asp Ile Cys Tyr Ala Thr Gln         200 205 210 aac cgc cag gtt gcc gtc aag gct atc gct gag cgc tgc gag ctg atg 787 Asn Arg Gln Val Ala Val Lys Ala Ile Ala Glu Arg Cys Glu Leu Met     215 220 225 att gtg gtc ggt tcc cgc aac tcc tcc aac tcg gtt cgt ctg gtt gag 835 Ile Val Val Gly Ser Arg Asn Ser Ser Asn Ser Val Arg Leu Val Glu 230 235 240 245 gtc gct aag caa aac ggt gcc gat aac gcc tac ctg gtg gat tac gcc 883 Val Ala Lys Gln Asn Gly Ala Asp Asn Ala Tyr Leu Val Asp Tyr Ala                 250 255 260 cgc gaa atc gac cca gca tgg ttc gaa ggc gta gag acc atc ggt atc 931 Arg Glu Ile Asp Pro Ala Trp Phe Glu Gly Val Glu Thr Ile Gly Ile             265 270 275 tcc tcc ggc gct tcc gtg cct gag atc ctc gtc cag ggc gtc att gag 979 Ser Ser Gly Ala Ser Val Pro Glu Ile Leu Val Gln Gly Val Ile Glu         280 285 290 cgc ctg gct gag ttc ggc tac gac gac gtc gag gaa gtc acc tcc gcc 1027 Arg Leu Ala Glu Phe Gly Tyr Asp Asp Val Glu Glu Val Thr Ser Ala     295 300 305 gct gag aag att gtt ttc gcg ctg cct cgc gtg ctg cgc cac aag aat 1075 Ala Glu Lys Ile Val Phe Ala Leu Pro Arg Val Leu Arg His Lys Asn 310 315 320 325 taattgcaag aatgaaaaat ccc 1098 <210> 100 <211> 325 <212> PRT <213> Corynebacterium glutamicum <400> 100 Met Ser Ser Pro Val Ile Ser Pro Glu Thr Lys Thr Gly Lys Lys Ile   1 5 10 15 Leu Leu Ala Ala Pro Arg Gly Tyr Cys Ala Gly Val Asp Arg Ala Val              20 25 30 Glu Thr Val Glu Arg Ala Leu Glu Glu Tyr Gly Ala Pro Ile Tyr Val          35 40 45 Arg Lys Glu Ile Val His Asn Arg Tyr Val Val Asp Thr Leu Ala Glu      50 55 60 Lys Gly Ala Ile Phe Val Asn Glu Ala Ser Glu Ala Pro Glu Gly Ala  65 70 75 80 Asn Met Val Phe Ser Ala His Gly Val Ser Pro Met Val His Glu Glu                  85 90 95 Ala Ala Ala Lys Asn Ile Lys Ala Ile Asp Ala Ala Cys Pro Leu Val             100 105 110 Thr Lys Val His Lys Glu Val Gln Arg Phe Asp Lys Gln Gly Phe His         115 120 125 Ile Leu Phe Ile Gly His Glu Gly His Glu Glu Val Glu Gly Thr Met     130 135 140 Gly His Ser Val Glu Lys Thr His Leu Val Asp Gly Val Ala Gly Ile 145 150 155 160 Ala Thr Leu Pro Glu Phe Leu Asn Asp Glu Pro Asn Leu Ile Trp Leu                 165 170 175 Ser Gln Thr Thr Leu Ser Val Asp Glu Thr Met Glu Ile Val Arg Glu             180 185 190 Leu Lys Val Lys Phe Pro Gln Leu Gln Asp Pro Pro Ser Asp Asp Ile         195 200 205 Cys Tyr Ala Thr Gln Asn Arg Gln Val Ala Val Lys Ala Ile Ala Glu     210 215 220 Arg Cys Glu Leu Met Ile Val Val Gly Ser Arg Asn Ser Ser Asn Ser 225 230 235 240 Val Arg Leu Val Glu Val Ala Lys Gln Asn Gly Ala Asp Asn Ala Tyr                 245 250 255 Leu Val Asp Tyr Ala Arg Glu Ile Asp Pro Ala Trp Phe Glu Gly Val             260 265 270 Glu Thr Ile Gly Ile Ser Ser Gly Ala Ser Val Pro Glu Ile Leu Val         275 280 285 Gln Gly Val Ile Glu Arg Leu Ala Glu Phe Gly Tyr Asp Asp Val Glu     290 295 300 Glu Val Thr Ser Ala Ala Glu Lys Ile Val Phe Ala Leu Pro Arg Val 305 310 315 320 Leu Arg His Lys Asn                 325 <210> 101 <211> 1131 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1108) <223> RXS01027 <400> 101 aatagatgga agtagttttt cattcactta tgtgcgcgtt tttaatctgg tttctaccaa 60 gaactgtgtg caccacaacg cggaaggtga atcgcaccca atg gca aat aag aac 115                                             Met Ala Asn Lys Asn                                               1 5 aat aag cct cat gag gtg gac aaa gac caa gat tca gcc atg ctg atc 163 Asn Lys Pro His Glu Val Asp Lys Asp Gln Asp Ser Ala Met Leu Ile                  10 15 20 aac ggt cgc ctg caa cag atc ccg gcg cgt ccc act gag gaa ttc acc 211 Asn Gly Arg Leu Gln Gln Ile Pro Ala Arg Pro Thr Glu Glu Phe Thr              25 30 35 cgc cca act ctt gca gca ggt gca gta ctg tgg cgc ggc gac atc acc 259 Arg Pro Thr Leu Ala Ala Gly Ala Val Leu Trp Arg Gly Asp Ile Thr          40 45 50 aac ccg gac agc atc gag gtc gct gtc atc cac cgc ccg cac tat gat 307 Asn Pro Asp Ser Ile Glu Val Ala Val Ile His Arg Pro His Tyr Asp      55 60 65 gac tgg tcc ctg gcc aag ggc aaa gtc gat ccc ggc gag tct att ccg 355 Asp Trp Ser Leu Ala Lys Gly Lys Val Asp Pro Gly Glu Ser Ile Pro  70 75 80 85 aca acc gcg gcc cgt gaa atc ctt gaa gaa act ggc tac gac atc cgt 403 Thr Thr Ala Ala Arg Glu Ile Leu Glu Glu Thr Gly Tyr Asp Ile Arg                  90 95 100 ctg ggc aag ctg atc ggc aag gtt act tac cct gtg ctc gac cga acc 451 Leu Gly Lys Leu Ile Gly Lys Val Thr Tyr Pro Val Leu Asp Arg Thr             105 110 115 aaa gtg gtc tac tac tgg act gcc cag gtt ctt ggt gga gag ttt gtc 499 Lys Val Val Tyr Tyr Trp Thr Ala Gln Val Leu Gly Gly Glu Phe Val         120 125 130 ccc aac gat gaa gtt gat gaa atc cgt tgg ctg tct gtt gat gaa gca 547 Pro Asn Asp Glu Val Asp Glu Ile Arg Trp Leu Ser Val Asp Glu Ala     135 140 145 tgc gag ttg ctc agc tac caa gta gat acc gaa gtt ctg gcc aag gca 595 Cys Glu Leu Leu Ser Tyr Gln Val Asp Thr Glu Val Leu Ala Lys Ala 150 155 160 165 gca aag cgt ttc cgc act cct tcc acc act cgg gtg ctg tat gtt cgc 643 Ala Lys Arg Phe Arg Thr Pro Ser Thr Thr Arg Val Leu Tyr Val Arg                 170 175 180 cat gct cat gca cat ggt cgc caa acc tgg ggt ggc gac gac aat aag 691 His Ala His Ala His Gly Arg Gln Thr Trp Gly Gly Asp Asp Asn Lys             185 190 195 cgc cca ttg gac aaa aag ggg cgt cga caa gca gaa atg ctc gta ccc 739 Arg Pro Leu Asp Lys Lys Gly Arg Arg Gln Ala Glu Met Leu Val Pro         200 205 210 atg ttg ttg ccc ttc aaa ccc acc gca att tac tcg gcg gtg ccc gat 787 Met Leu Leu Pro Phe Lys Pro Thr Ala Ile Tyr Ser Ala Val Pro Asp     215 220 225 cgc tgc caa gcc acc gcg ctc ccc ctt gcc gat gag ctc ggc ctc gac 835 Arg Cys Gln Ala Thr Ala Leu Pro Leu Ala Asp Glu Leu Gly Leu Asp 230 235 240 245 gtg tcc gtc aac cga ctg ttc ggc gac gac gcc tgg gaa acc gat ccc 883 Val Ser Val Asn Arg Leu Phe Gly Asp Asp Ala Trp Glu Thr Asp Pro                 250 255 260 gag gcc tgc aag aag cgc ttc acc gac gtg gtc gcg caa ggt ggc gtg 931 Glu Ala Cys Lys Lys Arg Phe Thr Asp Val Val Ala Gln Gly Gly Val             265 270 275 ccg atg atc gtt ggg cag ggc gac atc att ccg gaa atg atc aaa tgg 979 Pro Met Ile Val Gly Gln Gly Asp Ile Ile Pro Glu Met Ile Lys Trp         280 285 290 ttc tcc gag aac ggc acc ctc cct atc gat gag aag atc aag gcg aaa 1027 Phe Ser Glu Asn Gly Thr Leu Pro Ile Asp Glu Lys Ile Lys Ala Lys     295 300 305 aag ggc agc gtg tgg gtg ttg agc ttt cac gac ggt gtg ttc acc ggc 1075 Lys Gly Ser Val Trp Val Leu Ser Phe His Asp Gly Val Phe Thr Gly 310 315 320 325 gct gat tac ctg gcg agt tcc ctg ccg gtt aaa taggagcgcg tttaaggcct 1128 Ala Asp Tyr Leu Ala Ser Ser Leu Pro Val Lys                 330 335 cca 1131 <210> 102 <211> 336 <212> PRT <213> Corynebacterium glutamicum <400> 102 Met Ala Asn Lys Asn Asn Lys Pro His Glu Val Asp Lys Asp Gln Asp   1 5 10 15 Ser Ala Met Leu Ile Asn Gly Arg Leu Gln Gln Ile Pro Ala Arg Pro              20 25 30 Thr Glu Glu Phe Thr Arg Pro Thr Leu Ala Ala Gly Ala Val Leu Trp          35 40 45 Arg Gly Asp Ile Thr Asn Pro Asp Ser Ile Glu Val Ala Val Ile His      50 55 60 Arg Pro His Tyr Asp Asp Trp Ser Leu Ala Lys Gly Lys Val Asp Pro  65 70 75 80 Gly Glu Ser Ile Pro Thr Thr Ala Ala Arg Glu Ile Leu Glu Glu Thr                  85 90 95 Gly Tyr Asp Ile Arg Leu Gly Lys Leu Ile Gly Lys Val Thr Tyr Pro             100 105 110 Val Leu Asp Arg Thr Lys Val Val Tyr Tyr Trp Thr Ala Gln Val Leu         115 120 125 Gly Gly Glu Phe Val Pro Asn Asp Glu Val Asp Glu Ile Arg Trp Leu     130 135 140 Ser Val Asp Glu Ala Cys Glu Leu Leu Ser Tyr Gln Val Asp Thr Glu 145 150 155 160 Val Leu Ala Lys Ala Ala Lys Arg Phe Arg Thr Pro Ser Thr Thr Arg                 165 170 175 Val Leu Tyr Val Arg His Ala His Ala His Gly Arg Gln Thr Trp Gly             180 185 190 Gly Asp Asp Asn Lys Arg Pro Leu Asp Lys Lys Gly Arg Arg Gln Ala         195 200 205 Glu Met Leu Val Pro Met Leu Leu Pro Phe Lys Pro Thr Ala Ile Tyr     210 215 220 Ser Ala Val Pro Asp Arg Cys Gln Ala Thr Ala Leu Pro Leu Ala Asp 225 230 235 240 Glu Leu Gly Leu Asp Val Ser Val Asn Arg Leu Phe Gly Asp Asp Ala                 245 250 255 Trp Glu Thr Asp Pro Glu Ala Cys Lys Lys Arg Phe Thr Asp Val Val             260 265 270 Ala Gln Gly Gly Val Pro Met Ile Val Gly Gln Gly Asp Ile Ile Pro         275 280 285 Glu Met Ile Lys Trp Phe Ser Glu Asn Gly Thr Leu Pro Ile Asp Glu     290 295 300 Lys Ile Lys Ala Lys Lys Gly Ser Val Trp Val Leu Ser Phe His Asp 305 310 315 320 Gly Val Phe Thr Gly Ala Asp Tyr Leu Ala Ser Ser Leu Pro Val Lys                 325 330 335 <210> 103 <211> 651 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (628) <223> RXS01528 <400> 103 cacccaaacc caaacctctc agtcgaataa gcagaagtct caggacaacc gcaggggtaa 60 gggtcgtagg tctccaacca ggaggcgttc caacacgagg gtg aat cag gcg tgg 115                                             Val Asn Gln Ala Trp                                               1 5 cag cag tcc cgt ttg gtt act tct gat gag act tcc gca ggt ggt ctc 163 Gln Gln Ser Arg Leu Val Thr Ser Asp Glu Thr Ser Ala Gly Gly Leu                  10 15 20 gtg gtg tca ggt ttg gct gag gcg gtc aac gct aac aat gag gtt gat 211 Val Val Ser Gly Leu Ala Glu Ala Val Asn Ala Asn Asn Glu Val Asp              25 30 35 ctg tcg aag att tat gtt gcg ttg att ggt cgc ctt gat cgt cgt ggt 259 Leu Ser Lys Ile Tyr Val Ala Leu Ile Gly Arg Leu Asp Arg Arg Gly          40 45 50 cgt ttg ttg tgg tcg atg ccg aag ggc cat gtt gag cct ggt gag gat 307 Arg Leu Leu Trp Ser Met Pro Lys Gly His Val Glu Pro Gly Glu Asp      55 60 65 aag gct gcg act gct gag cgt gag gtg tgg gag gag acc ggc atc cac 355 Lys Ala Ala Thr Ala Glu Arg Glu Val Trp Glu Glu Thr Gly Ile His  70 75 80 85 ggt gag gtg ttc act gag ttg ggt gtg att gat tat tgg ttc gtt tcg 403 Gly Glu Val Phe Thr Glu Leu Gly Val Ile Asp Tyr Trp Phe Val Ser                  90 95 100 gaa ggg aag cgg atc cat aag acg gtg cat cat cat ttg ttg cgt tat 451 Glu Gly Lys Arg Ile His Lys Thr Val His His His Leu Leu Arg Tyr             105 110 115 gtt gat ggc gat ttg aat gat gag gat cca gaa gtc act gag gtg gcg 499 Val Asp Gly Asp Leu Asn Asp Glu Asp Pro Glu Val Thr Glu Val Ala         120 125 130 tgg att ccg gcg aat cag ttg att gag cat ttg gct ttt gcg gat gag 547 Trp Ile Pro Ala Asn Gln Leu Ile Glu His Leu Ala Phe Ala Asp Glu     135 140 145 cgg aag ttg gct agg cag gcg cat gat ttg ttg cct gag ttt gct ttg 595 Arg Lys Leu Ala Arg Gln Ala His Asp Leu Leu Pro Glu Phe Ala Leu 150 155 160 165 aag gaa aag gcg gag gga agg tcc acc cca agg tgattccgaa ccccaacccg 648 Lys Glu Lys Ala Glu Gly Arg Ser Thr Pro Arg                 170 175 aac 651 <210> 104 <211> 176 <212> PRT <213> Corynebacterium glutamicum <400> 104 Val Asn Gln Ala Trp Gln Gln Ser Arg Leu Val Thr Ser Asp Glu Thr   1 5 10 15 Ser Ala Gly Gly Leu Val Val Ser Gly Leu Ala Glu Ala Val Asn Ala              20 25 30 Asn Asn Glu Val Asp Leu Ser Lys Ile Tyr Val Ala Leu Ile Gly Arg          35 40 45 Leu Asp Arg Arg Gly Arg Leu Leu Trp Ser Met Pro Lys Gly His Val      50 55 60 Glu Pro Gly Glu Asp Lys Ala Ala Thr Ala Glu Arg Glu Val Trp Glu  65 70 75 80 Glu Thr Gly Ile His Gly Glu Val Phe Thr Glu Leu Gly Val Ile Asp                  85 90 95 Tyr Trp Phe Val Ser Glu Gly Lys Arg Ile His Lys Thr Val His His             100 105 110 His Leu Leu Arg Tyr Val Asp Gly Asp Leu Asn Asp Glu Asp Pro Glu         115 120 125 Val Thr Glu Val Ala Trp Ile Pro Ala Asn Gln Leu Ile Glu His Leu     130 135 140 Ala Phe Ala Asp Glu Arg Lys Leu Ala Arg Gln Ala His Asp Leu Leu 145 150 155 160 Pro Glu Phe Ala Leu Lys Glu Lys Ala Glu Gly Arg Ser Thr Pro Arg                 165 170 175 <210> 105 <211> 509 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (1) .. (486) <223> RXS01716 <400> 105 gaa gtc act cct gag gga ttc aaa gag atc acc cgt gaa aac acc atc 48 Glu Val Thr Pro Glu Gly Phe Lys Glu Ile Thr Arg Glu Asn Thr Ile   1 5 10 15 gtt cgc ctg ggc aaa ggc gtc gac gcc acc ggt cag cta gac ccc gag 96 Val Arg Leu Gly Lys Gly Val Asp Ala Thr Gly Gln Leu Asp Pro Glu              20 25 30 gca atc gag cgc act cgt gtc gct ttg gaa aac tac gtt gaa ctc atg 144 Ala Ile Glu Arg Thr Arg Val Ala Leu Glu Asn Tyr Val Glu Leu Met          35 40 45 gaa acc cat ggg gta gag gcc gta cga atg gtt gcc acc tcc gca acc 192 Glu Thr His Gly Val Glu Ala Val Arg Met Val Ala Thr Ser Ala Thr      50 55 60 cgc gat gcg tcc aac cgc gat gaa ttc ttt tcg atg acc cgc cag ctt 240 Arg Asp Ala Ser Asn Arg Asp Glu Phe Phe Ser Met Thr Arg Gln Leu  65 70 75 80 ctg tcc aag atc cgt cct gga tac caa gct gaa gta att tcc ggc gaa 288 Leu Ser Lys Ile Arg Pro Gly Tyr Gln Ala Glu Val Ile Ser Gly Glu                  85 90 95 gag gaa gct ctg ctg tcc ttc cga ggt gca atc gtt gac ctg cct gaa 336 Glu Glu Ala Leu Leu Ser Phe Arg Gly Ala Ile Val Asp Leu Pro Glu             100 105 110 gac caa ggt cct ttc tgt gtt atc gac ctt ggc ggt gga tcc act gag 384 Asp Gln Gly Pro Phe Cys Val Ile Asp Leu Gly Gly Gly Ser Thr Glu         115 120 125 ttc atc gtt ggc acc tac gac ggt gaa atc cta ggc tcc cac tca acc 432 Phe Ile Val Gly Thr Tyr Asp Gly Glu Ile Leu Gly Ser His Ser Thr     130 135 140 caa atg gga tgc gtg cgc ctg acc gaa cga atc atg cgc agc gac cca 480 Gln Met Gly Cys Val Arg Leu Thr Glu Arg Ile Met Arg Ser Asp Pro 145 150 155 160 ccc gac tgaaaccgaa gtggaaatcg ccc 509 Pro asp          <210> 106 <211> 162 <212> PRT <213> Corynebacterium glutamicum <400> 106 Glu Val Thr Pro Glu Gly Phe Lys Glu Ile Thr Arg Glu Asn Thr Ile   1 5 10 15 Val Arg Leu Gly Lys Gly Val Asp Ala Thr Gly Gln Leu Asp Pro Glu              20 25 30 Ala Ile Glu Arg Thr Arg Val Ala Leu Glu Asn Tyr Val Glu Leu Met          35 40 45 Glu Thr His Gly Val Glu Ala Val Arg Met Val Ala Thr Ser Ala Thr      50 55 60 Arg Asp Ala Ser Asn Arg Asp Glu Phe Phe Ser Met Thr Arg Gln Leu  65 70 75 80 Leu Ser Lys Ile Arg Pro Gly Tyr Gln Ala Glu Val Ile Ser Gly Glu                  85 90 95 Glu Glu Ala Leu Leu Ser Phe Arg Gly Ala Ile Val Asp Leu Pro Glu             100 105 110 Asp Gln Gly Pro Phe Cys Val Ile Asp Leu Gly Gly Gly Ser Thr Glu         115 120 125 Phe Ile Val Gly Thr Tyr Asp Gly Glu Ile Leu Gly Ser His Ser Thr     130 135 140 Gln Met Gly Cys Val Arg Leu Thr Glu Arg Ile Met Arg Ser Asp Pro 145 150 155 160 Pro asp          <210> 107 <211> 654 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (101) .. (631) <223> RXS01835 <400> 107 tcaacatcta ttcctcctgc gatttgcatg ggatatatat taaaaattct agccgaaagt 60 ttcctgcgtg aatacacttt ccccgcgcct tcgcaaagct atg aat act gcc gcg 115                                             Met Asn Thr Ala Ala                                               1 5 tgg gca cac cgc cac cac gta cgc aaa ggc ggt gga att ccg tat gtc 163 Trp Ala His Arg His His Val Arg Lys Gly Gly Gly Ile Pro Tyr Val                  10 15 20 agc cat ctt tat tca gtg atg tac ttg ctg gcc agc gtc act aat gat 211 Ser His Leu Tyr Ser Val Met Tyr Leu Leu Ala Ser Val Thr Asn Asp              25 30 35 gaa gat gtg ctc atc gcc ggg ctg ctc cac gac acc ctc gaa gac gta 259 Glu Asp Val Leu Ile Ala Gly Leu Leu His Asp Thr Leu Glu Asp Val          40 45 50 ccc gag gaa tac aat tct gcc caa ctt gaa gct gat ttt ggt ccg cgg 307 Pro Glu Glu Tyr Asn Ser Ala Gln Leu Glu Ala Asp Phe Gly Pro Arg      55 60 65 gtg cgc gag ttg gtg gaa gag ctc acc aaa cag ccc tta aaa agc tgg 355 Val Arg Glu Leu Val Glu Glu Leu Thr Lys Gln Pro Leu Lys Ser Trp  70 75 80 85 aaa gcg cgt gcc gac gct tac ctc ctg cac ctc agc gca ggt gcc agc 403 Lys Ala Arg Ala Asp Ala Tyr Leu Leu His Leu Ser Ala Gly Ala Ser                  90 95 100 tta gag gct gtc tta atc tcc acc gca gat aaa ctg cat aat ctc atg 451 Leu Glu Ala Val Leu Ile Ser Thr Ala Asp Lys Leu His Asn Leu Met             105 110 115 tcc atc ttg gat gac ctt gaa ata cac ggt gaa gat tta tgg caa cgc 499 Ser Ile Leu Asp Asp Leu Glu Ile His Gly Glu Asp Leu Trp Gln Arg         120 125 130 ttt aac gct ggc aaa gag cag caa atc tgg tgg tat agc gag gtt tat 547 Phe Asn Ala Gly Lys Glu Gln Gln Ile Trp Trp Tyr Ser Glu Val Tyr     135 140 145 cag ata tct ctc cag cgc tta ggg ttc aat gag ttg aat aaa caa ctg 595 Gln Ile Ser Leu Gln Arg Leu Gly Phe Asn Glu Leu Asn Lys Gln Leu 150 155 160 165 ggg ttg tgc gtc gaa aag ctc tta aag caa agc gcc taggcgctcg 641 Gly Leu Cys Val Glu Lys Leu Leu Lys Gln Ser Ala                 170 175 gcggcgtcga taa 654 <210> 108 <211> 177 <212> PRT <213> Corynebacterium glutamicum <400> 108 Met Asn Thr Ala Ala Trp Ala His Arg His His Val Arg Lys Gly Gly   1 5 10 15 Gly Ile Pro Tyr Val Ser His Leu Tyr Ser Val Met Tyr Leu Leu Ala              20 25 30 Ser Val Thr Asn Asp Glu Asp Val Leu Ile Ala Gly Leu Leu His Asp          35 40 45 Thr Leu Glu Asp Val Pro Glu Glu Tyr Asn Ser Ala Gln Leu Glu Ala      50 55 60 Asp Phe Gly Pro Arg Val Arg Glu Leu Val Glu Glu Leu Thr Lys Gln  65 70 75 80 Pro Leu Lys Ser Trp Lys Ala Arg Ala Asp Ala Tyr Leu Leu His Leu                  85 90 95 Ser Ala Gly Ala Ser Leu Glu Ala Val Leu Ile Ser Thr Ala Asp Lys             100 105 110 Leu His Asn Leu Met Ser Ile Leu Asp Asp Leu Glu Ile His Gly Glu         115 120 125 Asp Leu Trp Gln Arg Phe Asn Ala Gly Lys Glu Gln Gln Ile Trp Trp     130 135 140 Tyr Ser Glu Val Tyr Gln Ile Ser Leu Gln Arg Leu Gly Phe Asn Glu 145 150 155 160 Leu Asn Lys Gln Leu Gly Leu Cys Val Glu Lys Leu Leu Lys Gln Ser                 165 170 175 Ala      <210> 109 <211> 1050 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (1027) <223> RXS02497 <400> 109 tcgatgccgc cgctggcgaa gactcgggga aacctaaaaa taccgaagaa gaatttgacc 60 gattcacact ttgccaccct agaccgtcta acctttaggt gtg aga tta ggt gta 115                                             Val Arg Leu Gly Val                                               1 5 tta gat gtg ggc agc aat act gtc cac cta gtt gca gta gac gcg cgt 163 Leu Asp Val Gly Ser Asn Thr Val His Leu Val Ala Val Asp Ala Arg                  10 15 20 ccc ggt gga cac ccc acc ccg atg agc aat tgg cgt acc cca ctg cgc 211 Pro Gly Gly His Pro Thr Pro Met Ser Asn Trp Arg Thr Pro Leu Arg              25 30 35 ctt gtt gag ctt ctt gat gac tcc ggg gcg atc tcc gaa aag ggc atc 259 Leu Val Glu Leu Leu Asp Asp Ser Gly Ala Ile Ser Glu Lys Gly Ile          40 45 50 aac aaa ctc acc tca gca gtc ggg gaa gca gca gac cta gcg aaa acg 307 Asn Lys Leu Thr Ser Ala Val Gly Glu Ala Ala Asp Leu Ala Lys Thr      55 60 65 ctc ggc tgc gct gaa ctg atg cca ttt gct aca tcg gca gtc cgc tcc 355 Leu Gly Cys Ala Glu Leu Met Pro Phe Ala Thr Ser Ala Val Arg Ser  70 75 80 85 gcc acc aac agc gag gca gtg ctc gac cac gtg gag aag gaa acc ggc 403 Ala Thr Asn Ser Glu Ala Val Leu Asp His Val Glu Lys Glu Thr Gly                  90 95 100 gtc cgc ctg tcc atc ctt tcc ggt gaa gac gaa gca cgc caa act ttc 451 Val Arg Leu Ser Ile Leu Ser Gly Glu Asp Glu Ala Arg Gln Thr Phe             105 110 115 ctc gca gtt cga cgt tgg tat gga tgg tcc gca ggg cgc ata act aac 499 Leu Ala Val Arg Arg Trp Tyr Gly Trp Ser Ala Gly Arg Ile Thr Asn         120 125 130 ctc gac atc ggt ggc ggc tcc ctg gaa cta tcc tcc gga acc gac gaa 547 Leu Asp Ile Gly Gly Gly Ser Leu Glu Leu Ser Ser Gly Thr Asp Glu     135 140 145 tcc cca gac ctc gcg ttc tca ctg gat ctg ggt gcg ggc cgc ttg acc 595 Ser Pro Asp Leu Ala Phe Ser Leu Asp Leu Gly Ala Gly Arg Leu Thr 150 155 160 165 cac aac tgg ttc gac acc gat cca ccg gca cgt aag aaa atc aac ctc 643 His Asn Trp Phe Asp Thr Asp Pro Pro Ala Arg Lys Lys Ile Asn Leu                 170 175 180 ctg cgc gat tat atc gat gcg gaa ctt gca gaa ccc gcc cgc cag atg 691 Leu Arg Asp Tyr Ile Asp Ala Glu Leu Ala Glu Pro Ala Arg Gln Met             185 190 195 cgc acc cta ggg ccc gcg cgc ctg gca gtg gga aca tcc aaa act ttc 739 Arg Thr Leu Gly Pro Ala Arg Leu Ala Val Gly Thr Ser Lys Thr Phe         200 205 210 cgc acc ctg gca cga ctg act ggt gct gcg ccc tca tcc gca gga cca 787 Arg Thr Leu Ala Arg Leu Thr Gly Ala Ala Pro Ser Ser Ala Gly Pro     215 220 225 cac gtc acc cga acc ctc acc gcg ccg ggt ctg cgc cag ctg atc gca 835 His Val Thr Arg Thr Leu Thr Ala Pro Gly Leu Arg Gln Leu Ile Ala 230 235 240 245 ttt atc tca cga atg act gcg gcg gac cgc gct gag ctg gaa ggt atc 883 Phe Ile Ser Arg Met Thr Ala Ala Asp Arg Ala Glu Leu Glu Gly Ile                 250 255 260 agc tcg gat cgg tca cat cag atc gtg gca ggt gcg cta gtt gcg gaa 931 Ser Ser Asp Arg Ser His Gln Ile Val Ala Gly Ala Leu Val Ala Glu             265 270 275 gct gcg atg cgt gcg ttg gat att gac aag gta gaa att tgt ccg tgg 979 Ala Ala Met Arg Ala Leu Asp Ile Asp Lys Val Glu Ile Cys Pro Trp         280 285 290 gca ctt cgt gaa ggt gtg atc ctc acc agg atc gac aaa gga ctc gag 1027 Ala Leu Arg Glu Gly Val Ile Leu Thr Arg Ile Asp Lys Gly Leu Glu     295 300 305 taacatttac ccggaaagga gtt 1050 <210> 110 <211> 309 <212> PRT <213> Corynebacterium glutamicum <400> 110 Val Arg Leu Gly Val Leu Asp Val Gly Ser Asn Thr Val His Leu Val   1 5 10 15 Ala Val Asp Ala Arg Pro Gly Gly His Pro Thr Pro Met Ser Asn Trp              20 25 30 Arg Thr Pro Leu Arg Leu Val Glu Leu Leu Asp Asp Ser Gly Ala Ile          35 40 45 Ser Glu Lys Gly Ile Asn Lys Leu Thr Ser Ala Val Gly Glu Ala Ala      50 55 60 Asp Leu Ala Lys Thr Leu Gly Cys Ala Glu Leu Met Pro Phe Ala Thr  65 70 75 80 Ser Ala Val Arg Ser Ala Thr Asn Ser Glu Ala Val Leu Asp His Val                  85 90 95 Glu Lys Glu Thr Gly Val Arg Leu Ser Ile Leu Ser Gly Glu Asp Glu             100 105 110 Ala Arg Gln Thr Phe Leu Ala Val Arg Arg Trp Tyr Gly Trp Ser Ala         115 120 125 Gly Arg Ile Thr Asn Leu Asp Ile Gly Gly Gly Ser Leu Glu Leu Ser     130 135 140 Ser Gly Thr Asp Glu Ser Pro Asp Leu Ala Phe Ser Leu Asp Leu Gly 145 150 155 160 Ala Gly Arg Leu Thr His Asn Trp Phe Asp Thr Asp Pro Pro Ala Arg                 165 170 175 Lys Lys Ile Asn Leu Leu Arg Asp Tyr Ile Asp Ala Glu Leu Ala Glu             180 185 190 Pro Ala Arg Gln Met Arg Thr Leu Gly Pro Ala Arg Leu Ala Val Gly         195 200 205 Thr Ser Lys Thr Phe Arg Thr Leu Ala Arg Leu Thr Gly Ala Ala Pro     210 215 220 Ser Ser Ala Gly Pro His Val Thr Arg Thr Leu Thr Ala Pro Gly Leu 225 230 235 240 Arg Gln Leu Ile Ala Phe Ile Ser Arg Met Thr Ala Ala Asp Arg Ala                 245 250 255 Glu Leu Glu Gly Ile Ser Ser Asp Arg Ser His Gln Ile Val Ala Gly             260 265 270 Ala Leu Val Ala Glu Ala Ala Met Arg Ala Leu Asp Ile Asp Lys Val         275 280 285 Glu Ile Cys Pro Trp Ala Leu Arg Glu Gly Val Ile Leu Thr Arg Ile     290 295 300 Asp Lys Gly Leu Glu 305 <210> 111 <211> 534 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (511) <223> RXS02972 <400> 111 acctacgacg gtgaaatcct aggctcccac tcaacccaaa tgggatgcgt gcgcctgacc 60 gaacgaatca tgcgcagcga cccacccgac tgaaaccgaa gtg gaa atc gcc cgc 115                                             Val Glu Ile Ala Arg                                               1 5 gac tac gtt gca gaa cgc atc cag gaa gta aaa gcc atc gtc cca att 163 Asp Tyr Val Ala Glu Arg Ile Gln Glu Val Lys Ala Ile Val Pro Ile                  10 15 20 tca aag gca aaa acc ttt gtg gga tgc gca ggc acc ttc acc aca atc 211 Ser Lys Ala Lys Thr Phe Val Gly Cys Ala Gly Thr Phe Thr Thr Ile              25 30 35 tcc gcc tgg gtg caa ggc cta gaa agc tac gac cgc gac gcg atc cac 259 Ser Ala Trp Val Gln Gly Leu Glu Ser Tyr Asp Arg Asp Ala Ile His          40 45 50 ctc tct gca ctc aac ttc gat gca ctg cga gtt gtc acc gat gag atc 307 Leu Ser Ala Leu Asn Phe Asp Ala Leu Arg Val Val Thr Asp Glu Ile      55 60 65 att tca gaa tca tca tca cag cgc gcc agc aac cca gtt gtt gat cca 355 Ile Ser Glu Ser Ser Ser Gln Arg Ala Ser Asn Pro Val Val Asp Pro  70 75 80 85 ggt cgc gcc gac gtc atc ggt ggc gga tcc gtt gtt gtc caa gca gcg 403 Gly Arg Ala Asp Val Ile Gly Gly Gly Ser Val Val Val Gln Ala Ala                  90 95 100 atc gac tta gcc tcc aaa gaa gcc ggt gta gac tac atc att att tcc 451 Ile Asp Leu Ala Ser Lys Glu Ala Gly Val Asp Tyr Ile Ile Ile Ser             105 110 115 gaa aaa gac atc ctc gac ggc ctc atc ctt ggc ctg gta gaa gcc gac 499 Glu Lys Asp Ile Leu Asp Gly Leu Ile Leu Gly Leu Val Glu Ala Asp         120 125 130 tct ttg aag aaa taggacccta gttttaaacc act 534 Ser Leu Lys Lys     135 <210> 112 <211> 137 <212> PRT <213> Corynebacterium glutamicum <400> 112 Val Glu Ile Ala Arg Asp Tyr Val Ala Glu Arg Ile Gln Glu Val Lys   1 5 10 15 Ala Ile Val Pro Ile Ser Lys Ala Lys Thr Phe Val Gly Cys Ala Gly              20 25 30 Thr Phe Thr Thr Ile Ser Ala Trp Val Gln Gly Leu Glu Ser Tyr Asp          35 40 45 Arg Asp Ala Ile His Leu Ser Ala Leu Asn Phe Asp Ala Leu Arg Val      50 55 60 Val Thr Asp Glu Ile Ile Ser Glu Ser Ser Ser Gln Arg Ala Ser Asn  65 70 75 80 Pro Val Val Asp Pro Gly Arg Ala Asp Val Ile Gly Gly Gly Ser Val                  85 90 95 Val Val Gln Ala Ala Ile Asp Leu Ala Ser Lys Glu Ala Gly Val Asp             100 105 110 Tyr Ile Ile Ile Ser Glu Lys Asp Ile Leu Asp Gly Leu Ile Leu Gly         115 120 125 Leu Val Glu Ala Asp Ser Leu Lys Lys     130 135 <210> 113 <211> 636 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101) .. (613) <223> RXA02159 <400> 113 tgatggacca gcgtccaaag ttttcgatga agcagaaaac cgcctccacg ctcagaaagc 60 actgctggtg tggctgctgg ccaaccagcc gaggtaagac atg tcc ctt ggc tca 115                                             Met Ser Leu Gly Ser                                               1 5 acc ccg tca aca ccg gaa aac tta aat ccc gtg act cgc act gca cgc 163 Thr Pro Ser Thr Pro Glu Asn Leu Asn Pro Val Thr Arg Thr Ala Arg                  10 15 20 caa gct ctc att ttg cag att ttg gac aaa caa aaa gtc acc agc cag 211 Gln Ala Leu Ile Leu Gln Ile Leu Asp Lys Gln Lys Val Thr Ser Gln              25 30 35 gta caa ctg tct gaa ttg ctg ctg gat gaa ggc atc gat atc acc cag 259 Val Gln Leu Ser Glu Leu Leu Leu Asp Glu Gly Ile Asp Ile Thr Gln          40 45 50 gcc acc ttg tcc cga gat ctc gat gaa ctc ggt gca cgc aag gtt cgc 307 Ala Thr Leu Ser Arg Asp Leu Asp Glu Leu Gly Ala Arg Lys Val Arg      55 60 65 ccc gat ggg gga cgc gcc tac tac gcg gtc ggc cca gta gat agc atc 355 Pro Asp Gly Gly Arg Ala Tyr Tyr Ala Val Gly Pro Val Asp Ser Ile  70 75 80 85 gcc cgc gaa gat ctc cgg ggt ccg tcg gag aag ctg cgc cgc atg ctt 403 Ala Arg Glu Asp Leu Arg Gly Pro Ser Glu Lys Leu Arg Arg Met Leu                  90 95 100 gat gaa ctg ctg gtt tct aca gat cat tcc ggc aac atc gcg atg ctg 451 Asp Glu Leu Leu Val Ser Thr Asp His Ser Gly Asn Ile Ala Met Leu             105 110 115 cgc acc ccg ccg gga gct gcc cag tac ctg gca agt ttc atc gat agg 499 Arg Thr Pro Pro Gly Ala Ala Gln Tyr Leu Ala Ser Phe Ile Asp Arg         120 125 130 gtg ggg ctg aaa gaa gtc gtt ggc acc atc gct ggt gat gac acc gtt 547 Val Gly Leu Lys Glu Val Val Gly Thr Ile Ala Gly Asp Asp Thr Val     135 140 145 ttc gtt ctc gcc cgt gat ccg ctc aca ggt aaa gaa cta ggt gaa tta 595 Phe Val Leu Ala Arg Asp Pro Leu Thr Gly Lys Glu Leu Gly Glu Leu 150 155 160 165 ctc agc ggg cgc acc act taaagcgccc ctagttcaag gct 636 Leu Ser Gly Arg Thr Thr                 170 <210> 114 <211> 171 <212> PRT <213> Corynebacterium glutamicum <400> 114 Met Ser Leu Gly Ser Thr Pro Ser Thr Pro Glu Asn Leu Asn Pro Val   1 5 10 15 Thr Arg Thr Ala Arg Gln Ala Leu Ile Leu Gln Ile Leu Asp Lys Gln              20 25 30 Lys Val Thr Ser Gln Val Gln Leu Ser Glu Leu Leu Leu Asp Glu Gly          35 40 45 Ile Asp Ile Thr Gln Ala Thr Leu Ser Arg Asp Leu Asp Glu Leu Gly      50 55 60 Ala Arg Lys Val Arg Pro Asp Gly Gly Arg Ala Tyr Tyr Ala Val Gly  65 70 75 80 Pro Val Asp Ser Ile Ala Arg Glu Asp Leu Arg Gly Pro Ser Glu Lys                  85 90 95 Leu Arg Arg Met Leu Asp Glu Leu Leu Val Ser Thr Asp His Ser Gly             100 105 110 Asn Ile Ala Met Leu Arg Thr Pro Pro Gly Ala Ala Gln Tyr Leu Ala         115 120 125 Ser Phe Ile Asp Arg Val Gly Leu Lys Glu Val Val Gly Thr Ile Ala     130 135 140 Gly Asp Asp Thr Val Phe Val Leu Ala Arg Asp Pro Leu Thr Gly Lys 145 150 155 160 Glu Leu Gly Glu Leu Leu Ser Gly Arg Thr Thr                 165 170 <210> 115 <211> 486 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (463) <223> RXA02201 <400> 115 tctaccagcc aaatcatcaa ctcatagcga aggaatcaac ttcatgaata atcaaccatc 60 agtacttttc gtttgcgtcg gcaatggtgg aaaatctcaa atg gcc gca gcg cta 115                                             Met Ala Ala Ala Leu                                               1 5 gcc aaa aaa cat gcc ggg gac gct ctc aaa gtt tat tca gct ggc aca 163 Ala Lys Lys His Ala Gly Asp Ala Leu Lys Val Tyr Ser Ala Gly Thr                  10 15 20 aag cca ggt acg aaa tta aat caa cag tcc ctt gat tcc att gct gaa 211 Lys Pro Gly Thr Lys Leu Asn Gln Gln Ser Leu Asp Ser Ile Ala Glu              25 30 35 gtt ggc gca gat atg tct caa ggg ttt cca aag ggc att gac cag gag 259 Val Gly Ala Asp Met Ser Gln Gly Phe Pro Lys Gly Ile Asp Gln Glu          40 45 50 tta att aag cga gta gac cgc gtg gtc att ctt ggt gcc gaa gct caa 307 Leu Ile Lys Arg Val Asp Arg Val Val Ile Leu Gly Ala Glu Ala Gln      55 60 65 cta gaa atg cct atc gat gca aac ggc ata cta cag cgc tgg gta act 355 Leu Glu Met Pro Ile Asp Ala Asn Gly Ile Leu Gln Arg Trp Val Thr  70 75 80 85 gac gaa ccc tct gaa cgt gga att gaa ggt atg gaa cgc atg cgc ctg 403 Asp Glu Pro Ser Glu Arg Gly Ile Glu Gly Met Glu Arg Met Arg Leu                  90 95 100 gtc cga gat gat att gac gcc cga gtc caa aac ctc gtc gct gaa cta 451 Val Arg Asp Asp Ile Asp Ala Arg Val Gln Asn Leu Val Ala Glu Leu             105 110 115 acc caa aac gca tagcagtttt ctaatctcac aca 486 Thr Gln Asn Ala         120 <210> 116 <211> 121 <212> PRT <213> Corynebacterium glutamicum <400> 116 Met Ala Ala Ala Leu Ala Lys Lys His Ala Gly Asp Ala Leu Lys Val   1 5 10 15 Tyr Ser Ala Gly Thr Lys Pro Gly Thr Lys Leu Asn Gln Gln Ser Leu              20 25 30 Asp Ser Ile Ala Glu Val Gly Ala Asp Met Ser Gln Gly Phe Pro Lys          35 40 45 Gly Ile Asp Gln Glu Leu Ile Lys Arg Val Asp Arg Val Val Ile Leu      50 55 60 Gly Ala Glu Ala Gln Leu Glu Met Pro Ile Asp Ala Asn Gly Ile Leu  65 70 75 80 Gln Arg Trp Val Thr Asp Glu Pro Ser Glu Arg Gly Ile Glu Gly Met                  85 90 95 Glu Arg Met Arg Leu Val Arg Asp Asp Ile Asp Ala Arg Val Gln Asn             100 105 110 Leu Val Ala Glu Leu Thr Gln Asn Ala         115 120 <210> 117 <211> 510 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (101) .. (487) <223> RXA00599 <400> 117 gaacgatcgg ccctttgatt gaagtcccag tattagtcgg attggtttat gtcatgttgt 60 ggcttggacc aaaaatcttt aaaaaggaga atgcaggatc atg aaa tca gtt ttg 115                                             Met Lys Ser Val Leu                                               1 5 ttt gtg tgc gtc ggt aat ggc gga aaa tca cag atg gcg gcg gcg ctg 163 Phe Val Cys Val Gly Asn Gly Gly Lys Ser Gln Met Ala Ala Ala Leu                  10 15 20 gca cag aag tat gca tca gat tca gta gag atc cat tct gct gga acc 211 Ala Gln Lys Tyr Ala Ser Asp Ser Val Glu Ile His Ser Ala Gly Thr              25 30 35 aag cct gca cag ggg cta aac caa ttg tct gtg gaa tcc atc gct gag 259 Lys Pro Ala Gln Gly Leu Asn Gln Leu Ser Val Glu Ser Ile Ala Glu          40 45 50 gtg ggc gct gat atg tcg caa gga att ccc aaa gcg atc gat ccg gag 307 Val Gly Ala Asp Met Ser Gln Gly Ile Pro Lys Ala Ile Asp Pro Glu      55 60 65 ctg ctg cgc act gtc gat cgt gtg gtt att ttg ggc gat gac gca cag 355 Leu Leu Arg Thr Val Asp Arg Val Val Ile Leu Gly Asp Asp Ala Gln  70 75 80 85 gta gat atg cct gaa tct gca cag ggc gct ctt gag cgt tgg tca att 403 Val Asp Met Pro Glu Ser Ala Gln Gly Ala Leu Glu Arg Trp Ser Ile                  90 95 100 gag gaa ccg gat gct caa ggt atg gaa cgt atg cgt att gtg cgg gat 451 Glu Glu Pro Asp Ala Gln Gly Met Glu Arg Met Arg Ile Val Arg Asp             105 110 115 cag atc gat aac cga gtc caa gct ttg cta gcg gga taagcgccga 497 Gln Ile Asp Asn Arg Val Gln Ala Leu Leu Ala Gly         120 125 aaaaggggca tgt 510 <210> 118 <211> 129 <212> PRT <213> Corynebacterium glutamicum <400> 118 Met Lys Ser Val Leu Phe Val Cys Val Gly Asn Gly Gly Lys Ser Gln   1 5 10 15 Met Ala Ala Ala Leu Ala Gln Lys Tyr Ala Ser Asp Ser Val Glu Ile              20 25 30 His Ser Ala Gly Thr Lys Pro Ala Gln Gly Leu Asn Gln Leu Ser Val          35 40 45 Glu Ser Ile Ala Glu Val Gly Ala Asp Met Ser Gln Gly Ile Pro Lys      50 55 60 Ala Ile Asp Pro Glu Leu Leu Arg Thr Val Asp Arg Val Val Ile Leu  65 70 75 80 Gly Asp Asp Ala Gln Val Asp Met Pro Glu Ser Ala Gln Gly Ala Leu                  85 90 95 Glu Arg Trp Ser Ile Glu Glu Pro Asp Ala Gln Gly Met Glu Arg Met             100 105 110 Arg Ile Val Arg Asp Gln Ile Asp Asn Arg Val Gln Ala Leu Leu Ala         115 120 125 Gly      <210> 119 <211> 1221 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1198) <223> RXA00600 <400> 119 cggagttaat gagcggtagg tggatgggtg cggtcatgtc cgtcattata tattgacgca 60 catcgatatt gaaggtattt ttatatcggc aaacatcaat atg att gaa ggc tgg 115                                             Met Ile Glu Gly Trp                                               1 5 ctc atg acc ctt act aaa gag cat tcg aca cct cga gcg gct ggc tca 163 Leu Met Thr Leu Thr Lys Glu His Ser Thr Pro Arg Ala Ala Gly Ser                  10 15 20 atg tcg ttt ctt gac cgc tgg tta gct gcc tgg att ttc ttg gct atg 211 Met Ser Phe Leu Asp Arg Trp Leu Ala Ala Trp Ile Phe Leu Ala Met              25 30 35 gct gct ggg ttg tta atc ggc aag gtc ttt cca gga att ggg gcg ctt 259 Ala Ala Gly Leu Leu Ile Gly Lys Val Phe Pro Gly Ile Gly Ala Leu          40 45 50 ttg agc gcg gtg gaa att ggt gga att tcc att cca att gct atc ggt 307 Leu Ser Ala Val Glu Ile Gly Gly Ile Ser Ile Pro Ile Ala Ile Gly      55 60 65 ttg atc gtc atg atg tat cca cct ttg gcc aag gtg cgc tac gac aaa 355 Leu Ile Val Met Met Tyr Pro Pro Leu Ala Lys Val Arg Tyr Asp Lys  70 75 80 85 act aaa gaa atc agc aca gac cgc gct ctc atg gtg gtg tcg att atg 403 Thr Lys Glu Ile Ser Thr Asp Arg Ala Leu Met Val Val Ser Ile Met                  90 95 100 ttg aac tgg atc gtt gga cca gca ctt atg ttt agc ctg gcg tgg ctg 451 Leu Asn Trp Ile Val Gly Pro Ala Leu Met Phe Ser Leu Ala Trp Leu             105 110 115 ttt ctt cca gat caa cca gag ctt cgc act ggg cta att atc gtg ggc 499 Phe Leu Pro Asp Gln Pro Glu Leu Arg Thr Gly Leu Ile Val Gly         120 125 130 ctt gcg cgc tgt atc gcg atg gtt ttg gta tgg agt gat ctc gct tgt 547 Leu Ala Arg Cys Ile Ala Met Val Leu Val Trp Ser Asp Leu Ala Cys     135 140 145 ggt gac cgg gaa gca act gct gtg ctg gtt gca atc aac tcg gtg ttc 595 Gly Asp Arg Glu Ala Thr Ala Val Leu Val Ala Ile Asn Ser Val Phe 150 155 160 165 cag atc ctt atg ttc ggt gtg ctt ggt tgg ttt tac ctg cag att ctt 643 Gln Ile Leu Met Phe Gly Val Leu Gly Trp Phe Tyr Leu Gln Ile Leu                 170 175 180 ccc tcg tgg ctg gga tta gac acc acg tcg gtg act ttc tct gtg gta 691 Pro Ser Trp Leu Gly Leu Asp Thr Thr Ser Val Val Phe Ser Val Val             185 190 195 tca atc gtg act tcc gtt ctc gtg ttc ttg ggc ata cca ctt gta gct 739 Ser Ile Val Thr Ser Val Leu Val Phe Leu Gly Ile Pro Leu Val Ala         200 205 210 gga gtt tta tct cgc gtc att ggt gaa aaa aca aag gga cgg cgc tgg 787 Gly Val Leu Ser Arg Val Ile Gly Glu Lys Thr Lys Gly Arg Arg Trp     215 220 225 tac gag gac acg ttc ctg cct aag att tca ccc ttg gcg ctg att ggc 835 Tyr Glu Asp Thr Phe Leu Pro Lys Ile Ser Pro Leu Ala Leu Ile Gly 230 235 240 245 ttg cta tac aca att gtt ctg ctg ttt tcg ttg cag ggg gat gaa atc 883 Leu Leu Tyr Thr Ile Val Leu Leu Phe Ser Leu Gln Gly Asp Glu Ile                 250 255 260 aca gcg cag cct tgg aca gta gct cgt ctt gca ttg ccg ctg ctg atg 931 Thr Ala Gln Pro Trp Thr Val Ala Arg Leu Ala Leu Pro Leu Leu Met             265 270 275 tac ttt gtg ggc atg ttt ttc att tcc ctg gtg gta tcc aaa ctg tcc 979 Tyr Phe Val Gly Met Phe Phe Ile Ser Leu Val Val Ser Lys Leu Ser         280 285 290 ggg tta act tat gag cga gct gct tcc gtg tct ttt act gca gca gga 1027 Gly Leu Thr Tyr Glu Arg Ala Ala Ser Val Ser Phe Thr Ala Ala Gly     295 300 305 aac aac ttt gaa tta gcg att gcg gta tcg atc gga acc ttt ggt gcg 1075 Asn Asn Phe Glu Leu Ala Ile Ala Val Ser Ile Gly Thr Phe Gly Ala 310 315 320 325 aca tca ccg cag gca tta gct gga acg atc ggc cct ttg att gaa gtc 1123 Thr Ser Pro Gln Ala Leu Ala Gly Thr Ile Gly Pro Leu Ile Glu Val                 330 335 340 cca gta tta gtc gga ttg gtt tat gtc atg ttg tgg ctt gga cca aaa 1171 Pro Val Leu Val Gly Leu Val Tyr Val Met Leu Trp Leu Gly Pro Lys             345 350 355 atc ttt aaa aag gag aat gca gga tca tgaaatcagt tttgtttgtg 1218 Ile Phe Lys Lys Glu Asn Ala Gly Ser         360 365 tgc 1221 <210> 120 <211> 366 <212> PRT <213> Corynebacterium glutamicum <400> 120 Met Ile Glu Gly Trp Leu Met Thr Leu Thr Lys Glu His Ser Thr Pro   1 5 10 15 Arg Ala Ala Gly Ser Met Ser Phe Leu Asp Arg Trp Leu Ala Ala Trp              20 25 30 Ile Phe Leu Ala Met Ala Ala Gly Leu Leu Ile Gly Lys Val Phe Pro          35 40 45 Gly Ile Gly Ala Leu Leu Ser Ala Val Glu Ile Gly Gly Ile Ser Ile      50 55 60 Pro Ile Ala Ile Gly Leu Ile Val Met Met Tyr Pro Pro Leu Ala Lys  65 70 75 80 Val Arg Tyr Asp Lys Thr Lys Glu Ile Ser Thr Asp Arg Ala Leu Met                  85 90 95 Val Val Ser Ile Met Leu Asn Trp Ile Val Gly Pro Ala Leu Met Phe             100 105 110 Ser Leu Ala Trp Leu Phe Leu Pro Asp Gln Pro Glu Leu Arg Thr Gly         115 120 125 Leu Ile Ile Val Gly Leu Ala Arg Cys Ile Ala Met Val Leu Val Trp     130 135 140 Ser Asp Leu Ala Cys Gly Asp Arg Glu Ala Thr Ala Val Leu Val Ala 145 150 155 160 Ile Asn Ser Val Phe Gln Ile Leu Met Phe Gly Val Leu Gly Trp Phe                 165 170 175 Tyr Leu Gln Ile Leu Pro Ser Trp Leu Gly Leu Asp Thr Thr Ser Val             180 185 190 Thr Phe Ser Val Val Ser Ile Val Thr Ser Val Leu Val Phe Leu Gly         195 200 205 Ile Pro Leu Val Ala Gly Val Leu Ser Arg Val Ile Gly Glu Lys Thr     210 215 220 Lys Gly Arg Arg Trp Tyr Glu Asp Thr Phe Leu Pro Lys Ile Ser Pro 225 230 235 240 Leu Ala Leu Ile Gly Leu Leu Tyr Thr Ile Val Leu Leu Phe Ser Leu                 245 250 255 Gln Gly Asp Glu Ile Thr Ala Gln Pro Trp Thr Val Ala Arg Leu Ala             260 265 270 Leu Pro Leu Leu Met Tyr Phe Val Gly Met Phe Phe Ile Ser Leu Val         275 280 285 Val Ser Lys Leu Ser Gly Leu Thr Tyr Glu Arg Ala Ala Ser Val Ser     290 295 300 Phe Thr Ala Ala Gly Asn Asn Phe Glu Leu Ala Ile Ala Val Ser Ile 305 310 315 320 Gly Thr Phe Gly Ala Thr Ser Pro Gln Ala Leu Ala Gly Thr Ile Gly                 325 330 335 Pro Leu Ile Glu Val Pro Val Leu Val Gly Leu Val Tyr Val Met Leu             340 345 350 Trp Leu Gly Pro Lys Ile Phe Lys Lys Glu Asn Ala Gly Ser         355 360 365 <210> 121 <211> 1233 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1210) <223> RXA02200 <400> 121 attgtgtgga gagtggtcat aaatccacta tatattgacg aatgtcgata ttgaaagtat 60 tttgaatatc gacaggtatc aatataccga aaggtgtcgc atg aca aac tca act 115                                             Met Thr Asn Ser Thr                                               1 5 cag acg cgg gcc aag cca gcc cga atc tca ttt ctt gat aaa tac att 163 Gln Thr Arg Ala Lys Pro Ala Arg Ile Ser Phe Leu Asp Lys Tyr Ile                  10 15 20 cca ctt tgg att att ttg gcg atg gcg ttt ggg cta ttt tta ggc cgg 211 Pro Leu Trp Ile Ile Leu Ala Met Ala Phe Gly Leu Phe Leu Gly Arg              25 30 35 agc gtt tcg gga ctc tca ggc ttt cta ggc gca atg gaa gtc gga ggg 259 Ser Val Ser Gly Leu Ser Gly Phe Leu Gly Ala Met Glu Val Gly Gly          40 45 50 atc tcc ttg cca atc gct tta ggc ctc ctt gta atg atg tac cca ccg 307 Ile Ser Leu Pro Ile Ala Leu Gly Leu Leu Val Met Met Tyr Pro Pro      55 60 65 ttg gcc aaa gtt cgg tat gac aaa act aaa caa att gcc act gat aag 355 Leu Ala Lys Val Arg Tyr Asp Lys Thr Lys Gln Ile Ala Thr Asp Lys  70 75 80 85 cat ttg atg ggc gtg tca ctc att ctc aat tgg gtg gtg ggt cct gcc 403 His Leu Met Gly Val Ser Leu Ile Leu Asn Trp Val Val Gly Pro Ala                  90 95 100 tta atg ttc gcg cta gct tgg ttg ttc ctc cca gac caa ccg gaa tta 451 Leu Met Phe Ala Leu Ala Trp Leu Phe Leu Pro Asp Gln Pro Glu Leu             105 110 115 cga acc ggc ctg att att gta gga ctc gca cga tgt att gcg atg gtc 499 Arg Thr Gly Leu Ile Ile Val Gly Leu Ala Arg Cys Ile Ala Met Val         120 125 130 ttg gtt tgg tct gat atg tcc tgt gga gac cgc gag gct aca gca gtt 547 Leu Val Trp Ser Asp Met Ser Cys Gly Asp Arg Glu Ala Thr Ala Val     135 140 145 ctc gta gcc att aat tca gtt ttt caa gtc gca atg ttt ggt gca ctt 595 Leu Val Ala Ile Asn Ser Val Phe Gln Val Ala Met Phe Gly Ala Leu 150 155 160 165 ggc tgg ttc tat ctg caa gtt tta cca tcc tgg cta gga tta cca act 643 Gly Trp Phe Tyr Leu Gln Val Leu Pro Ser Trp Leu Gly Leu Pro Thr                 170 175 180 acc acc gct caa ttc tct ttc tgg tca att gtg act tcg gtt ttg gtg 691 Thr Thr Ala Gln Phe Ser Phe Trp Ser Ile Val Thr Ser Val Leu Val             185 190 195 ttc ctc gga ata cct cta ctt gct gga gtt ttc tcg cga att att ggc 739 Phe Leu Gly Ile Pro Leu Leu Ala Gly Val Phe Ser Arg Ile Ile Gly         200 205 210 gaa aag atc aag gga cgt gag tgg tat gaa caa aag ttc ctt ccg gca 787 Glu Lys Ile Lys Gly Arg Glu Trp Tyr Glu Gln Lys Phe Leu Pro Ala     215 220 225 atc tct cca ttt gca cta atc ggt ctg ctt tat acg atc gtc ttg ttg 835 Ile Ser Pro Phe Ala Leu Ile Gly Leu Leu Tyr Thr Ile Val Leu Leu 230 235 240 245 ttt tca ttg caa ggt gat cag atc gtc tct caa cca tgg gct gta gtt 883 Phe Ser Leu Gln Gly Asp Gln Ile Val Ser Gln Pro Trp Ala Val Val                 250 255 260 cgt ctc gcg ata cca ttg gtt atc tat ttc gtt gga atg ttt ttc att 931 Arg Leu Ala Ile Pro Leu Val Ile Tyr Phe Val Gly Met Phe Phe Ile             265 270 275 tca ctc att gcg tca aaa cta tct ggc atg aac tat gca aag tct gca 979 Ser Leu Ile Ala Ser Lys Leu Ser Gly Met Asn Tyr Ala Lys Ser Ala         280 285 290 tcc gtc tct ttc act gca gct ggc aac aat ttt gaa ctt gcg att gcg 1027 Ser Val Ser Phe Thr Ala Ala Gly Asn Asn Phe Glu Leu Ala Ile Ala     295 300 305 gtg tcg atc gga acg ttt ggc gca act tct gca cag gct atg gca gga 1075 Val Ser Ile Gly Thr Phe Gly Ala Thr Ser Ala Gln Ala Met Ala Gly 310 315 320 325 acg att ggt ccc ttg att gaa att cca gta ctt gtc ggc ttg gtc tac 1123 Thr Ile Gly Pro Leu Ile Glu Ile Pro Val Leu Val Gly Leu Val Tyr                 330 335 340 gcc atg ctg tgg cta ggc ccc aag ttg ttc cca aat gac ccc acg ctg 1171 Ala Met Leu Trp Leu Gly Pro Lys Leu Phe Pro Asn Asp Pro Thr Leu             345 350 355 cca tca tca gct cgt tct acc agc caa atc atc aac tca tagcgaagga 1220 Pro Ser Ser Ala Arg Ser Thr Ser Gln Ile Ile Asn Ser         360 365 370 atcaacttca tga 1233 <210> 122 <211> 370 <212> PRT <213> Corynebacterium glutamicum <400> 122 Met Thr Asn Ser Thr Gln Thr Arg Ala Lys Pro Ala Arg Ile Ser Phe   1 5 10 15 Leu Asp Lys Tyr Ile Pro Leu Trp Ile Ile Leu Ala Met Ala Phe Gly              20 25 30 Leu Phe Leu Gly Arg Ser Val Ser Gly Leu Ser Gly Phe Leu Gly Ala          35 40 45 Met Glu Val Gly Gly Ile Ser Leu Pro Ile Ala Leu Gly Leu Leu Val      50 55 60 Met Met Tyr Pro Pro Leu Ala Lys Val Arg Tyr Asp Lys Thr Lys Gln  65 70 75 80 Ile Ala Thr Asp Lys His Leu Met Gly Val Ser Leu Ile Leu Asn Trp                  85 90 95 Val Val Gly Pro Ala Leu Met Phe Ala Leu Ala Trp Leu Phe Leu Pro             100 105 110 Asp Gln Pro Glu Leu Arg Thr Gly Leu Ile Ile Val Gly Leu Ala Arg         115 120 125 Cys Ile Ala Met Val Leu Val Trp Ser Asp Met Ser Cys Gly Asp Arg     130 135 140 Glu Ala Thr Ala Val Leu Val Ala Ile Asn Ser Val Phe Gln Val Ala 145 150 155 160 Met Phe Gly Ala Leu Gly Trp Phe Tyr Leu Gln Val Leu Pro Ser Trp                 165 170 175 Leu Gly Leu Pro Thr Thr Thr Ala Gln Phe Ser Phe Trp Ser Ile Val             180 185 190 Thr Ser Val Leu Val Phe Leu Gly Ile Pro Leu Leu Ala Gly Val Phe         195 200 205 Ser Arg Ile Ile Gly Glu Lys Ile Lys Gly Arg Glu Trp Tyr Glu Gln     210 215 220 Lys Phe Leu Pro Ala Ile Ser Pro Phe Ala Leu Ile Gly Leu Leu Tyr 225 230 235 240 Thr Ile Val Leu Leu Phe Ser Leu Gln Gly Asp Gln Ile Val Ser Gln                 245 250 255 Pro Trp Ala Val Val Arg Leu Ala Ile Pro Leu Val Ile Tyr Phe Val             260 265 270 Gly Met Phe Phe Ile Ser Leu Ile Ala Ser Lys Leu Ser Gly Met Asn         275 280 285 Tyr Ala Lys Ser Ala Ser Val Ser Phe Thr Ala Ala Gly Asn Asn Phe     290 295 300 Glu Leu Ala Ile Ala Val Ser Ile Gly Thr Phe Gly Ala Thr Ser Ala 305 310 315 320 Gln Ala Met Ala Gly Thr Ile Gly Pro Leu Ile Glu Ile Pro Val Leu                 325 330 335 Val Gly Leu Val Tyr Ala Met Leu Trp Leu Gly Pro Lys Leu Phe Pro             340 345 350 Asn Asp Pro Thr Leu Pro Ser Ser Ala Arg Ser Thr Ser Gln Ile Ile         355 360 365 Asn ser     370 <210> 123 <211> 762 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101) .. (739) <223> RXA02202 <400> 123 cgctgaacta acccaaaacg catagcagtt ttctaatctc acacatcttc aacaccgtta 60 aatctattgg tttccccgta aaatcttcga aaggaagaac atg acc ggg caa gct 115                                             Met Thr Gly Gln Ala                                               1 5 gca cca aac ttg cat acc aat att ttg aac cgt atc gca aat gaa ctg 163 Ala Pro Asn Leu His Thr Asn Ile Leu Asn Arg Ile Ala Asn Glu Leu                  10 15 20 gcg ttg acc tat caa gga gtt ttc tct gca gag act atc aac cgc tat 211 Ala Leu Thr Tyr Gln Gly Val Phe Ser Ala Glu Thr Ile Asn Arg Tyr              25 30 35 att ttt gaa tcg tat gtg tcg ttg gcg aga aca gca aaa atc cat acg 259 Ile Phe Glu Ser Tyr Val Ser Leu Ala Arg Thr Ala Lys Ile His Thr          40 45 50 cac ctg cca att ttg gca gaa ggt ttt gct aaa gac cgg ctg cac gca 307 His Leu Pro Ile Leu Ala Glu Gly Phe Ala Lys Asp Arg Leu His Ala      55 60 65 ctt gcg gta gct gaa ggt aag gtg gct tca cct gtg cct cag gtc cta 355 Leu Ala Val Ala Glu Gly Lys Val Ala Ser Pro Val Pro Gln Val Leu  70 75 80 85 ttt att tgc gtc cac aac gca ggt cgt tca caa att gct tcg gcg ttg 403 Phe Ile Cys Val His Asn Ala Gly Arg Ser Gln Ile Ala Ser Ala Leu                  90 95 100 ttg tct cac tat gcc ggt agt tct gta gag gta cgt tct gca ggt tct 451 Leu Ser His Tyr Ala Gly Ser Ser Val Glu Val Arg Ser Ala Gly Ser             105 110 115 tta cct gct tct gaa att cac cca ctg gtg ttg gaa att ttg tca gag 499 Leu Pro Ala Ser Glu Ile His Pro Leu Val Leu Glu Ile Leu Ser Glu         120 125 130 cga gga gtg aac att tct gat gca ttt ccg aaa ccg cta acc gat gat 547 Arg Gly Val Asn Ile Ser Asp Ala Phe Pro Lys Pro Leu Thr Asp Asp     135 140 145 gtt att cgc gca tct gac tat gtc ata aca atg gga tgt gga gat gtg 595 Val Ile Arg Ala Ser Asp Tyr Val Ile Thr Met Gly Cys Gly Asp Val 150 155 160 165 tgc cca atg tat cca gga aag cac tat ctc gat tgg gag ctc gct gat 643 Cys Pro Met Tyr Pro Gly Lys His Tyr Leu Asp Trp Glu Leu Ala Asp                 170 175 180 ccg tca gat gaa ggt gag gac aag ata cag gaa ata att gag gaa att 691 Pro Ser Asp Glu Gly Glu Asp Lys Ile Gln Glu Ile Ile Glu Glu Ile             185 190 195 gac ggt cga atc cgc gag ctt tgg aaa agc att caa tta tcg caa aac 739 Asp Gly Arg Ile Arg Glu Leu Trp Lys Ser Ile Gln Leu Ser Gln Asn         200 205 210 taggcagtca aaggtctggc acc 762 <210> 124 <211> 213 <212> PRT <213> Corynebacterium glutamicum <400> 124 Met Thr Gly Gln Ala Ala Pro Asn Leu His Thr Asn Ile Leu Asn Arg   1 5 10 15 Ile Ala Asn Glu Leu Ala Leu Thr Tyr Gln Gly Val Phe Ser Ala Glu              20 25 30 Thr Ile Asn Arg Tyr Ile Phe Glu Ser Tyr Val Ser Leu Ala Arg Thr          35 40 45 Ala Lys Ile His Thr His Leu Pro Ile Leu Ala Glu Gly Phe Ala Lys      50 55 60 Asp Arg Leu His Ala Leu Ala Val Ala Glu Gly Lys Val Ala Ser Pro  65 70 75 80 Val Pro Gln Val Leu Phe Ile Cys Val His Asn Ala Gly Arg Ser Gln                  85 90 95 Ile Ala Ser Ala Leu Leu Ser His Tyr Ala Gly Ser Ser Val Glu Val             100 105 110 Arg Ser Ala Gly Ser Leu Pro Ala Ser Glu Ile His Pro Leu Val Leu         115 120 125 Glu Ile Leu Ser Glu Arg Gly Val Asn Ile Ser Asp Ala Phe Pro Lys     130 135 140 Pro Leu Thr Asp Asp Val Ile Arg Ala Ser Asp Tyr Val Ile Thr Met 145 150 155 160 Gly Cys Gly Asp Val Cys Pro Met Tyr Pro Gly Lys His Tyr Leu Asp                 165 170 175 Trp Glu Leu Ala Asp Pro Ser Asp Glu Gly Glu Asp Lys Ile Gln Glu             180 185 190 Ile Ile Glu Glu Ile Asp Gly Arg Ile Arg Glu Leu Trp Lys Ser Ile         195 200 205 Gln Leu Ser Gln Asn     210 <210> 125 <211> 1002 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (979) <223> RXA02205 <400> 125 gcccaaaccg agcctggata cccgcaaacc acttgaaccg accattcgct gtttcacgcc 60 caccacacta ctgaggtcat aaggtagtac ggtagatcgg gtg aat gaa gag ata 115                                             Val Asn Glu Glu Ile                                               1 5 acc ctc cta gcc gca gca gca gat cct gcc gca act gaa aat att ggc 163 Thr Leu Leu Ala Ala Ala Ala Asp Pro Ala Ala Thr Glu Asn Ile Gly                  10 15 20 tgg gta caa acc att gtg ctc tcc atc gtt caa ggc ctc aca gag ttc 211 Trp Val Gln Thr Ile Val Leu Ser Ile Val Gln Gly Leu Thr Glu Phe              25 30 35 ctg ccg atc agc tcc agc gga cac ctc cga atc atc tct gag ctg ttc 259 Leu Pro Ile Ser Ser Ser Gly His Leu Arg Ile Ile Ser Glu Leu Phe          40 45 50 tgg ggt gcc gat gcc ggc gcg tcc ttt acc gcc gtg gtt cag ctt ggt 307 Trp Gly Ala Asp Ala Gly Ala Ser Phe Thr Ala Val Val Gln Leu Gly      55 60 65 acc gaa gcc gca gtg ctg gtg ttt ttt gcc aag gaa atc tgg caa atc 355 Thr Glu Ala Ala Val Leu Val Phe Phe Ala Lys Glu Ile Trp Gln Ile  70 75 80 85 atc aca ggt tgg ttc gct ggc gta ttc aat aag gaa cgc cgc gga ttt 403 Ile Thr Gly Trp Phe Ala Gly Val Phe Asn Lys Glu Arg Arg Gly Phe                  90 95 100 gaa tac cgc atg ggc tgg atg atc att gtt gcc acc att ccc gtc gtg 451 Glu Tyr Arg Met Gly Trp Met Ile Ile Val Ala Thr Ile Pro Val Val             105 110 115 atc ttg ggt gtg ttg ggc aag gac ctg atc cgt gag gcg ctg cga aat 499 Ile Leu Gly Val Leu Gly Lys Asp Leu Ile Arg Glu Ala Leu Arg Asn         120 125 130 atg tgg atc act gca tcc gtg ctg atc ctg ttc tcc ctg gtg ttc att 547 Met Trp Ile Thr Ala Ser Val Leu Ile Leu Phe Ser Leu Val Phe Ile     135 140 145 ttg gcc gag aag atg ggc aag aag gaa cgc gac tac gac aaa ctg acc 595 Leu Ala Glu Lys Met Gly Lys Lys Glu Arg Asp Tyr Asp Lys Leu Thr 150 155 160 165 atg aaa gat gcc atc atc atg ggt ctt gca cag tgt ctt gcg ctg atc 643 Met Lys Asp Ala Ile Ile Met Gly Leu Ala Gln Cys Leu Ala Leu Ile                 170 175 180 cct ggc gtg tct cgc tcc ggc ggc acc atc tct gct ggt ttg ttc ctt 691 Pro Gly Val Ser Arg Ser Gly Gly Thr Ile Ser Ala Gly Leu Phe Leu             185 190 195 ggt ctc aag cgt gaa gta gcc acc aag ttc tcc ttc ctg ctg gca atc 739 Gly Leu Lys Arg Glu Val Ala Thr Lys Phe Ser Phe Leu Leu Ala Ile         200 205 210 cct gca gtg ctt ggc tcc ggt ttg tac tcc ctg cct gac gct ttt gcg 787 Pro Ala Val Leu Gly Ser Gly Leu Tyr Ser Leu Pro Asp Ala Phe Ala     215 220 225 cca agc tcc gga caa gct gcc tcc ggc cta cag ctc acc gtg ggt acc 835 Pro Ser Ser Gly Gln Ala Ala Ser Gly Leu Gln Leu Thr Val Gly Thr 230 235 240 245 ctg gtt gcc ttc gta gtt ggc tac att tcc att gcg tgg ctg atg aag 883 Leu Val Ala Phe Val Val Gly Tyr Ile Ser Ile Ala Trp Leu Met Lys                 250 255 260 ttc gtg gca aac cac tcc ttc agc tgg ttt gct gca tac cgt att cct 931 Phe Val Ala Asn His Ser Phe Ser Trp Phe Ala Ala Tyr Arg Ile Pro             265 270 275 gca ggt ctg ctc gtg atg ctg ctg ctc gca ctg ggc atg ctc aac cca 979 Ala Gly Leu Leu Val Met Leu Leu Leu Ala Leu Gly Met Leu Asn Pro         280 285 290 taaaattcct gtacatctta aaa 1002 <210> 126 <211> 293 <212> PRT <213> Corynebacterium glutamicum <400> 126 Val Asn Glu Glu Ile Thr Leu Leu Ala Ala Ala Ala Asp Pro Ala Ala   1 5 10 15 Thr Glu Asn Ile Gly Trp Val Gln Thr Ile Val Leu Ser Ile Val Gln              20 25 30 Gly Leu Thr Glu Phe Leu Pro Ile Ser Ser Ser Gly His Leu Arg Ile          35 40 45 Ile Ser Glu Leu Phe Trp Gly Ala Asp Ala Gly Ala Ser Phe Thr Ala      50 55 60 Val Val Gln Leu Gly Thr Glu Ala Ala Val Leu Val Phe Phe Ala Lys  65 70 75 80 Glu Ile Trp Gln Ile Ile Thr Gly Trp Phe Ala Gly Val Phe Asn Lys                  85 90 95 Glu Arg Arg Gly Phe Glu Tyr Arg Met Gly Trp Met Ile Ile Val Ala             100 105 110 Thr Ile Pro Val Val Ile Leu Gly Val Leu Gly Lys Asp Leu Ile Arg         115 120 125 Glu Ala Leu Arg Asn Met Trp Ile Thr Ala Ser Val Leu Ile Leu Phe     130 135 140 Ser Leu Val Phe Ile Leu Ala Glu Lys Met Gly Lys Lys Glu Arg Asp 145 150 155 160 Tyr Asp Lys Leu Thr Met Lys Asp Ala Ile Ile Met Gly Leu Ala Gln                 165 170 175 Cys Leu Ala Leu Ile Pro Gly Val Ser Arg Ser Gly Gly Thr Ile Ser             180 185 190 Ala Gly Leu Phe Leu Gly Leu Lys Arg Glu Val Ala Thr Lys Phe Ser         195 200 205 Phe Leu Leu Ala Ile Pro Ala Val Leu Gly Ser Gly Leu Tyr Ser Leu     210 215 220 Pro Asp Ala Phe Ala Pro Ser Ser Gly Gln Ala Ala Ser Gly Leu Gln 225 230 235 240 Leu Thr Val Gly Thr Leu Val Ala Phe Val Val Gly Tyr Ile Ser Ile                 245 250 255 Ala Trp Leu Met Lys Phe Val Ala Asn His Ser Phe Ser Trp Phe Ala             260 265 270 Ala Tyr Arg Ile Pro Ala Gly Leu Leu Val Met Leu Leu Leu Ala Leu         275 280 285 Gly Met Leu Asn Pro     290 <210> 127 <211> 975 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (952) <223> RXA00900 <400> 127 gtgcggtggc tgcgctggtc gctagtgtgg tgtgcgcgct ggcgccgtcg ataagcgtat 60 tagtgatcgc acgcctggtg caggggcttg gcggcggtgc gtg cgt ggt att gcg 115                                             Val Arg Gly Ile Ala                                               1 5 cgc gcg atc gtg cca gac ctt gaa cgc gga caa aag gct gcg cac gcc 163 Arg Ala Ile Val Pro Asp Leu Glu Arg Gly Gln Lys Ala Ala His Ala                  10 15 20 ttt gca ctg ctg atg att att cag gga att gct ccc gtg gta gct ccg 211 Phe Ala Leu Leu Met Ile Ile Gln Gly Ile Ala Pro Val Val Ala Pro              25 30 35 ctc att ggt ggt gtg ctg gtc ggg cct ttt ggc tgg cgg gga att ttc 259 Leu Ile Gly Gly Val Leu Val Gly Pro Phe Gly Trp Arg Gly Ile Phe          40 45 50 tgg gca ctt gca ctg gtg aat ttt gcg cag ctg ctt gtt gct ttg ctg 307 Trp Ala Leu Ala Leu Val Asn Phe Ala Gln Leu Leu Val Ala Leu Leu      55 60 65 cag att aag gag tcg aag cca gtt gaa gag cgt acc gca gca gga ctt 355 Gln Ile Lys Glu Ser Lys Pro Val Glu Glu Arg Thr Ala Ala Gly Leu  70 75 80 85 ggc gga atg ctg tcc aac tat gtc ttt gtg ctg aag aat cct caa ttt 403 Gly Gly Met Leu Ser Asn Tyr Val Phe Val Leu Lys Asn Pro Gln Phe                  90 95 100 ttg gca tat gta ttc aca ttg ggg ctg tct ttt ggg gcg atg ttc tcc 451 Leu Ala Tyr Val Phe Thr Leu Gly Leu Ser Phe Gly Ala Met Phe Ser             105 110 115 tac att tcg gcg tcg ccg ttc gtg ctg cag aat caa atg ggc att ccg 499 Tyr Ile Ser Ala Ser Pro Phe Val Leu Gln Asn Gln Met Gly Ile Pro         120 125 130 gta ctg ctg tat tcc att att ttc gga gtg aat gct ttt ggt ttg att 547 Val Leu Leu Tyr Ser Ile Ile Phe Gly Val Asn Ala Phe Gly Leu Ile     135 140 145 gtg ggc gga atg gtc aat agg cga ctt ctg cag cgg att cat cca cac 595 Val Gly Gly Met Val Asn Arg Arg Leu Leu Gln Arg Ile His Pro His 150 155 160 165 cgc atc atg caa act gtg ctg gcc agt ttt act gtg ctg tgt gcg ctt 643 Arg Ile Met Gln Thr Val Leu Ala Ser Phe Thr Val Leu Cys Ala Leu                 170 175 180 ttg ctg att gaa gtg ctg ttt att aat tgg ata ccg ctg ttc ctg ttg 691 Leu Leu Ile Glu Val Leu Phe Ile Asn Trp Ile Pro Leu Phe Leu Leu             185 190 195 ctg ctg ttt ctt atc gtt tcc cat att ccg atg gtt atg gct aac gcg 739 Leu Leu Phe Leu Ile Val Ser His Ile Pro Met Val Met Ala Asn Ala         200 205 210 aca gct ctg gga act gaa gtg gtg cga agc agg gcg gga tcg ggt tct 787 Thr Ala Leu Gly Thr Glu Val Val Arg Ser Arg Ala Gly Ser Gly Ser     215 220 225 gca att ttg ggt ttc gtg caa ttc acg atg ggt gct ttg gtg agt tca 835 Ala Ile Leu Gly Phe Val Gln Phe Thr Met Gly Ala Leu Val Ser Ser 230 235 240 245 ctg gtc gga tta ggc tct gat aag gct ttg act atg gga atc gca atg 883 Leu Val Gly Leu Gly Ser Asp Lys Ala Leu Thr Met Gly Ile Ala Met                 250 255 260 act gct tgt gca ctg ctg gcg tgt ggg tgt gcg tac ctg gca ggt cga 931 Thr Ala Cys Ala Leu Leu Ala Cys Gly Cys Ala Tyr Leu Ala Gly Arg             265 270 275 aaa ggt att cca gaa atg aag tagctctagg tggcgtttta agg 975 Lys Gly Ile Pro Glu Met Lys         280 <210> 128 <211> 284 <212> PRT <213> Corynebacterium glutamicum <400> 128 Val Arg Gly Ile Ala Arg Ala Ile Val Pro Asp Leu Glu Arg Gly Gln   1 5 10 15 Lys Ala Ala His Ala Phe Ala Leu Leu Met Ile Ile Gln Gly Ile Ala              20 25 30 Pro Val Val Ala Pro Leu Ile Gly Gly Val Leu Val Gly Pro Phe Gly          35 40 45 Trp Arg Gly Ile Phe Trp Ala Leu Ala Leu Val Asn Phe Ala Gln Leu      50 55 60 Leu Val Ala Leu Leu Gln Ile Lys Glu Ser Lys Pro Val Glu Glu Arg  65 70 75 80 Thr Ala Ala Gly Leu Gly Gly Met Leu Ser Asn Tyr Val Phe Val Leu                  85 90 95 Lys Asn Pro Gln Phe Leu Ala Tyr Val Phe Thr Leu Gly Leu Ser Phe             100 105 110 Gly Ala Met Phe Ser Tyr Ile Ser Ala Ser Pro Phe Val Leu Gln Asn         115 120 125 Gln Met Gly Ile Pro Val Leu Leu Tyr Ser Ile Ile Phe Gly Val Asn     130 135 140 Ala Phe Gly Leu Ile Val Gly Gly Met Val Asn Arg Arg Leu Leu Gln 145 150 155 160 Arg Ile His Pro His Arg Ile Met Gln Thr Val Leu Ala Ser Phe Thr                 165 170 175 Val Leu Cys Ala Leu Leu Leu Ile Glu Val Leu Phe Ile Asn Trp Ile             180 185 190 Pro Leu Phe Leu Leu Leu Leu Phe Leu Ile Val Ser His Ile Pro Met         195 200 205 Val Met Ala Asn Ala Thr Ala Leu Gly Thr Glu Val Val Arg Ser Arg     210 215 220 Ala Gly Ser Gly Ser Ala Ile Leu Gly Phe Val Gln Phe Thr Met Gly 225 230 235 240 Ala Leu Val Ser Ser Leu Val Gly Leu Gly Ser Asp Lys Ala Leu Thr                 245 250 255 Met Gly Ile Ala Met Thr Ala Cys Ala Leu Leu Ala Cys Gly Cys Ala             260 265 270 Tyr Leu Ala Gly Arg Lys Gly Ile Pro Glu Met Lys         275 280 <210> 129 <211> 537 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (514) <223> RXN00901 <400> 129 ttttaagtat tggtgctatc ttccggtgct gatggtacct gaatgaaaat ttctaattaa 60 aaataccccc aaatcttcga tatagataca cgagacagtg atg cag aaa aaa caa 115                                             Met Gln Lys Lys Gln                                               1 5 cag ctg agc acc gcc ctg att atg gga ttg gca tta ttg tca gcc agc 163 Gln Leu Ser Thr Ala Leu Ile Met Gly Leu Ala Leu Leu Ser Ala Ser                  10 15 20 tcc gcg cta gcg act gat atg tat ttg ccg gca atg cct ggt att gcg 211 Ser Ala Leu Ala Thr Asp Met Tyr Leu Pro Ala Met Pro Gly Ile Ala              25 30 35 gaa gat ttg ggg aca act gca ccg atg gtg cag tta act ctt tct tcc 259 Glu Asp Leu Gly Thr Thr Ala Pro Met Val Gln Leu Thr Leu Ser Ser          40 45 50 ttt atg gct gga atg gcg att ggc caa ttg atc att ggt cct ttg tcg 307 Phe Met Ala Gly Met Ala Ile Gly Gln Leu Ile Ile Gly Pro Leu Ser      55 60 65 gat caa ttg gga agg aaa ggc ctg ctc gtt gca ggt gcg gtg gct gcg 355 Asp Gln Leu Gly Arg Lys Gly Leu Leu Val Ala Gly Ala Val Ala Ala  70 75 80 85 ctg gtc gct agt gtg gtg tgc gcg ctg gcg ccg tcg ata agc gta tta 403 Leu Val Ala Ser Val Val Cys Ala Leu Ala Pro Ser Ile Ser Val Leu                  90 95 100 gtg atc gca cgc ctg gtg cag ggg ctt ggc ggc ggt gcg tgc gtg gta 451 Val Ile Ala Arg Leu Val Gln Gly Leu Gly Gly Gly Ala Cys Val Val             105 110 115 ttg cgc gcg cga tcg tgc cag acc ttg aac gcg gac aaa agg ctg cgc 499 Leu Arg Ala Arg Ser Cys Gln Thr Leu Asn Ala Asp Lys Arg Leu Arg         120 125 130 acg cct ttg cac tgc tgatgattat tcagggaatt gct 537 Thr Pro Leu His Cys     135 <210> 130 <211> 138 <212> PRT <213> Corynebacterium glutamicum <400> 130 Met Gln Lys Lys Gln Gln Leu Ser Thr Ala Leu Ile Met Gly Leu Ala   1 5 10 15 Leu Leu Ser Ala Ser Ser Ala Leu Ala Thr Asp Met Tyr Leu Pro Ala              20 25 30 Met Pro Gly Ile Ala Glu Asp Leu Gly Thr Thr Ala Pro Met Val Gln          35 40 45 Leu Thr Leu Ser Ser Phe Met Ala Gly Met Ala Ile Gly Gln Leu Ile      50 55 60 Ile Gly Pro Leu Ser Asp Gln Leu Gly Arg Lys Gly Leu Leu Val Ala  65 70 75 80 Gly Ala Val Ala Ala Leu Val Ala Ser Val Val Cys Ala Leu Ala Pro                  85 90 95 Ser Ile Ser Val Leu Val Ile Ala Arg Leu Val Gln Gly Leu Gly Gly             100 105 110 Gly Ala Cys Val Val Leu Arg Ala Arg Ser Cys Gln Thr Leu Asn Ala         115 120 125 Asp Lys Arg Leu Arg Thr Pro Leu His Cys     130 135 <210> 131 <211> 501 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101) .. (478) <223> FRXA00901 <400> 131 acctgaatga aaatttctaa ttaaaaatac ccccaaatct tcgatataga tacacgagac 60 agtgatgcag aaaaaacaac agctgagcac cgccctgatt atg gga ttg gca tta 115                                             Met Gly Leu Ala Leu                                               1 5 ttg tca gcc agc tcc gcg cta gcg act gat atg tat ttg ccg gca atg 163 Leu Ser Ala Ser Ser Ala Leu Ala Thr Asp Met Tyr Leu Pro Ala Met                  10 15 20 cct ggt att gcg gaa gat ttg ggg aca act gca ccg atg gtg cag tta 211 Pro Gly Ile Ala Glu Asp Leu Gly Thr Thr Ala Pro Met Val Gln Leu              25 30 35 act ctt tct tcc ttt atg gct gga atg gcg att ggc caa ttg atc att 259 Thr Leu Ser Ser Phe Met Ala Gly Met Ala Ile Gly Gln Leu Ile Ile          40 45 50 ggt cct ttg tcg gat caa ttg gga agg aaa ggc ctg ctc gtt gca ggt 307 Gly Pro Leu Ser Asp Gln Leu Gly Arg Lys Gly Leu Leu Val Ala Gly      55 60 65 gcg gtg gct gcg ctg gtc gct agt gtg gtg tgc gcg ctg gcg ccg tcg 355 Ala Val Ala Ala Leu Val Ala Ser Val Val Cys Ala Leu Ala Pro Ser  70 75 80 85 ata agc gta tta gtg atc gca cgc ctg gtg cag ggg ctt ggc ggc ggt 403 Ile Ser Val Leu Val Ile Ala Arg Leu Val Gln Gly Leu Gly Gly Gly                  90 95 100 gcg tgc gtg gta ttg cgc gcg cga tcg tgc cag acc ttg aac gcg gac 451 Ala Cys Val Val Leu Arg Ala Arg Ser Cys Gln Thr Leu Asn Ala Asp             105 110 115 aaa agg ctg cgc acg cct ttg cac tgc tgatgattat tcagggaatt 498 Lys Arg Leu Arg Thr Pro Leu His Cys         120 125 gct 501 <210> 132 <211> 126 <212> PRT <213> Corynebacterium glutamicum <400> 132 Met Gly Leu Ala Leu Leu Ser Ala Ser Ser Ala Leu Ala Thr Asp Met   1 5 10 15 Tyr Leu Pro Ala Met Pro Gly Ile Ala Glu Asp Leu Gly Thr Thr Ala              20 25 30 Pro Met Val Gln Leu Thr Leu Ser Ser Phe Met Ala Gly Met Ala Ile          35 40 45 Gly Gln Leu Ile Ile Gly Pro Leu Ser Asp Gln Leu Gly Arg Lys Gly      50 55 60 Leu Leu Val Ala Gly Ala Val Ala Ala Leu Val Ala Ser Val Val Cys  65 70 75 80 Ala Leu Ala Pro Ser Ile Ser Val Leu Val Ile Ala Arg Leu Val Gln                  85 90 95 Gly Leu Gly Gly Gly Ala Cys Val Val Leu Arg Ala Arg Ser Cys Gln             100 105 110 Thr Leu Asn Ala Asp Lys Arg Leu Arg Thr Pro Leu His Cys         115 120 125 <210> 133 <211> 1299 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (1276) <223> RXA00289 <133> 133 cctccccata agttcactca agcaagttct cccgaacaga ttcacccgag aagtcgacag 60 accccattaa acagcccgat tcaagaaagg cttcgcagcc atg agc acc acc 115                                             Met Ser Thr Thr Thr                                               1 5 gcg ccc gaa gca cgg ttt cct gtc gtc cct ttg acc gcc atg agt ttc 163 Ala Pro Glu Ala Arg Phe Pro Val Val Pro Leu Thr Ala Met Ser Phe                  10 15 20 gcg gca ttt gtt tat gtc acg ttc gag atg ttt gca gtt ggc ctc atc 211 Ala Ala Phe Val Tyr Val Thr Phe Glu Met Phe Ala Val Gly Leu Ile              25 30 35 aag ccg atg gcc agc gat ctt gga gtg tca gaa tcc agc atc ggc ctg 259 Lys Pro Met Ala Ser Asp Leu Gly Val Ser Glu Ser Ser Ile Gly Leu          40 45 50 ttg atg act gtg tat gcg act gtc gtt gcc gtg gtg acg atc cct gcc 307 Leu Met Thr Val Tyr Ala Thr Val Val Ala Val Val Thr Ile Pro Ala      55 60 65 atg ttg tgg gtt tct cga ttt aac aag cgc aca gtt ttc ctg att act 355 Met Leu Trp Val Ser Arg Phe Asn Lys Arg Thr Val Phe Leu Ile Thr  70 75 80 85 ctg gca ttt ttg gcc acg ggc att gtt gtt cag gca ctg acc gtt aat 403 Leu Ala Phe Leu Ala Thr Gly Ile Val Val Gln Ala Leu Thr Val Asn                  90 95 100 tat gga atg cta gcc atc ggc cgc act atc gca gca ttg act cac ggg 451 Tyr Gly Met Leu Ala Ile Gly Arg Thr Ile Ala Ala Leu Thr His Gly             105 110 115 gtg ttt tgg gca ctt gtt ggg cca atg gca gcg cgt atg tcc cca ggt 499 Val Phe Trp Ala Leu Val Gly Pro Met Ala Ala Arg Met Ser Pro Gly         120 125 130 cac act ggt cgt gca gta ggc gtt gtg tcg att gga tca acc atg gcg 547 His Thr Gly Arg Ala Val Gly Val Val Ser Ile Gly Ser Thr Met Ala     135 140 145 ctg gtc gtt ggt tct ccg ctg gca aca tgg atc ggt gaa ctc atc gga 595 Leu Val Val Gly Ser Pro Leu Ala Thr Trp Ile Gly Glu Leu Ile Gly 150 155 160 165 tgg cgt cct gcc acc tgg att ctt ggt gcg ctg acc att gcg gcc gtg 643 Trp Arg Pro Ala Thr Trp Ile Leu Gly Ala Leu Thr Ile Ala Ala Val                 170 175 180 gct gta ctc att cca acc gtt cca tca ctg cca cca ctt cca gac acg 691 Ala Val Leu Ile Pro Thr Val Pro Ser Leu Pro Pro Leu Pro Asp Thr             185 190 195 gaa tca gag tcc aaa gaa aag aaa tcc ctt cca tgg ggt ctc att tcc 739 Glu Ser Glu Ser Lys Glu Lys Lys Ser Leu Pro Trp Gly Leu Ile Ser         200 205 210 ctg gtc att ttc ctt ctc ctt gcc gtc acc ggt gtt ttt gct gcc tac 787 Leu Val Ile Phe Leu Leu Leu Ala Val Thr Gly Val Phe Ala Ala Tyr     215 220 225 acc tac ctt ggc ctc atc atc gct gaa aca gca ggg gac agc ttc gtg 835 Thr Tyr Leu Gly Leu Ile Ile Ala Glu Thr Ala Gly Asp Ser Phe Val 230 235 240 245 tcc att ggc ttg ttc gcc ttc ggt gca ctc gga ctc att ggc gtg aca 883 Ser Ile Gly Leu Phe Ala Phe Gly Ala Leu Gly Leu Ile Gly Val Thr                 250 255 260 gtg gca acc cga act gtg gat caa cgc atg ctg cgt gga agt gtt cac 931 Val Ala Thr Arg Thr Val Asp Gln Arg Met Leu Arg Gly Ser Val His             265 270 275 acc acc act ttg ttt gtc att gct gca att ctc gga cag atc gca ttc 979 Thr Thr Thr Leu Phe Val Ile Ala Ala Ile Leu Gly Gln Ile Ala Phe         280 285 290 gga tta gag ggc aca cta gcc gta gta gct atc ttc ctt gca gtc acc 1027 Gly Leu Glu Gly Thr Leu Ala Val Val Ala Ile Phe Leu Ala Val Thr     295 300 305 gtg ttt ggt gga gca tac ggc gct ctc cca acc ctg gga acc acc atc 1075 Val Phe Gly Gly Ala Tyr Gly Ala Leu Pro Thr Leu Gly Thr Thr Ile 310 315 320 325 ttc ctc cat gcg ggt cgc gac cac cca gat act gca tcc tcc att tat 1123 Phe Leu His Ala Gly Arg Asp His Pro Asp Thr Ala Ser Ser Ile Tyr                 330 335 340 gtg gtc act tac caa gtg ggt atc gcg tct ggc gcg gca ctt ggc gcg 1171 Val Val Thr Tyr Gln Val Gly Ile Ala Ser Gly Ala Ala Leu Gly Ala             345 350 355 atg gct gtg gat gcc gat tgg gtt gct ggc act ttg tgg atc atg gct 1219 Met Ala Val Asp Ala Asp Trp Val Ala Gly Thr Leu Trp Ile Met Ala         360 365 370 gga ctg tca ttg gct tcc acg ttg gcc ttg gcg ctg tgg tcc cgc ccg 1267 Gly Leu Ser Leu Ala Ser Thr Leu Ala Leu Ala Leu Trp Ser Arg Pro     375 380 385 cta ctg aag tagcagccca aattcagccc act 1299 Leu Leu Lys 390 <210> 134 <211> 392 <212> PRT <213> Corynebacterium glutamicum <400> 134 Met Ser Thr Thr Thr Ala Pro Glu Ala Arg Phe Pro Val Val Pro Leu   1 5 10 15 Thr Ala Met Ser Phe Ala Ala Phe Val Tyr Val Thr Phe Glu Met Phe              20 25 30 Ala Val Gly Leu Ile Lys Pro Met Ala Ser Asp Leu Gly Val Ser Glu          35 40 45 Ser Ser Ile Gly Leu Leu Met Thr Val Tyr Ala Thr Val Val Ala Val      50 55 60 Val Thr Ile Pro Ala Met Leu Trp Val Ser Arg Phe Asn Lys Arg Thr  65 70 75 80 Val Phe Leu Ile Thr Leu Ala Phe Leu Ala Thr Gly Ile Val Val Gln                  85 90 95 Ala Leu Thr Val Asn Tyr Gly Met Leu Ala Ile Gly Arg Thr Ile Ala             100 105 110 Ala Leu Thr His Gly Val Phe Trp Ala Leu Val Gly Pro Met Ala Ala         115 120 125 Arg Met Ser Pro Gly His Thr Gly Arg Ala Val Gly Val Val Ser Ile     130 135 140 Gly Ser Thr Met Ala Leu Val Val Gly Ser Pro Leu Ala Thr Trp Ile 145 150 155 160 Gly Glu Leu Ile Gly Trp Arg Pro Ala Thr Trp Ile Leu Gly Ala Leu                 165 170 175 Thr Ile Ala Ala Val Ala Val Leu Ile Pro Thr Val Pro Ser Leu Pro             180 185 190 Pro Leu Pro Asp Thr Glu Ser Glu Ser Lys Glu Lys Lys Ser Leu Pro         195 200 205 Trp Gly Leu Ile Ser Leu Val Ile Phe Leu Leu Leu Ala Val Thr Gly     210 215 220 Val Phe Ala Ala Tyr Thr Tyr Leu Gly Leu Ile Ile Ala Glu Thr Ala 225 230 235 240 Gly Asp Ser Phe Val Ser Ile Gly Leu Phe Ala Phe Gly Ala Leu Gly                 245 250 255 Leu Ile Gly Val Thr Val Ala Thr Arg Thr Val Asp Gln Arg Met Leu             260 265 270 Arg Gly Ser Val His Thr Thr Thr Leu Phe Val Ile Ala Ala Ile Leu         275 280 285 Gly Gln Ile Ala Phe Gly Leu Glu Gly Thr Leu Ala Val Val Ala Ile     290 295 300 Phe Leu Ala Val Thr Val Phe Gly Gly Ala Tyr Gly Ala Leu Pro Thr 305 310 315 320 Leu Gly Thr Thr Ile Phe Leu His Ala Gly Arg Asp His Pro Asp Thr                 325 330 335 Ala Ser Ser Ile Tyr Val Val Thr Tyr Gln Val Gly Ile Ala Ser Gly             340 345 350 Ala Ala Leu Gly Ala Met Ala Val Asp Ala Asp Trp Val Ala Gly Thr         355 360 365 Leu Trp Ile Met Ala Gly Leu Ser Leu Ala Ser Thr Leu Ala Leu Ala     370 375 380 Leu Trp Ser Arg Pro Leu Leu Lys 385 390 <210> 135 <211> 420 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (397) <223> RXN01984 <400> 135 aggaaatgtc tcacgtcaca accttttgaa aggtggctaa gtacgcacat ttgttgtctg 60 caatagtgcc ggtgagggag ctgtccgata ttgtgcttac atg cac gaa tct gga 115                                             Met His Glu Ser Gly                                               1 5 aaa aat cct gtc aag gtt gtc gac tcg cag gca cca caa gga cgc ggt 163 Lys Asn Pro Val Lys Val Val Asp Ser Gln Ala Pro Gln Gly Arg Gly                  10 15 20 ggg cat atc ggc gga cat atc aaa cgc cgc ccg att cct agg caa acg 211 Gly His Ile Gly Gly His Ile Lys Arg Arg Pro Ile Pro Arg Gln Thr              25 30 35 gaa att tcc gag gtt cgt cga tat atc gtc atg act gcc ctc gca ctc 259 Glu Ile Ser Glu Val Arg Arg Tyr Ile Val Met Thr Ala Leu Ala Leu          40 45 50 ggt ggc ttc gcc atc ggt gtg acg gaa ttt gtc tcc atg ggt ctg ctc 307 Gly Gly Phe Ala Ile Gly Val Thr Glu Phe Val Ser Met Gly Leu Leu      55 60 65 agc gcg atc gcc tcc gac ttt gag atc tcc gaa gac caa gcc gga cac 355 Ser Ala Ile Ala Ser Asp Phe Glu Ile Ser Glu Asp Gln Ala Gly His  70 75 80 85 atc atc acc atc tac gcc ctc gcg tgg ttg tgg gtg ccc cgc 397 Ile Ile Thr Ile Tyr Ala Leu Ala Trp Leu Trp Val Pro Arg                  90 95 tgatcacagc gtttaccggc aaa 420 <210> 136 <211> 99 <212> PRT <213> Corynebacterium glutamicum <400> 136 Met His Glu Ser Gly Lys Asn Pro Val Lys Val Val Asp Ser Gln Ala   1 5 10 15 Pro Gln Gly Arg Gly Gly His Ile Gly Gly His Ile Lys Arg Arg Pro              20 25 30 Ile Pro Arg Gln Thr Glu Ile Ser Glu Val Arg Arg Tyr Ile Val Met          35 40 45 Thr Ala Leu Ala Leu Gly Gly Phe Ala Ile Gly Val Thr Glu Phe Val      50 55 60 Ser Met Gly Leu Leu Ser Ala Ile Ala Ser Asp Phe Glu Ile Ser Glu  65 70 75 80 Asp Gln Ala Gly His Ile Ile Thr Ile Tyr Ala Leu Ala Trp Leu Trp                  85 90 95 Val Pro Arg              <210> 137 <211> 379 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (379) <223> FRXA01984 <400> 137 aggaaatgtc tcacgtcaca accttttgaa aggtggctaa gtacgcacat ttgttgtctg 60 caatagtgcc ggtgagggag ctgtccgata ttgtgcttac atg cac gaa tct gga 115                                             Met His Glu Ser Gly                                               1 5 aaa aat cct gtc aag gtt gtc gac tcg cag gca cca caa gga cgc ggt 163 Lys Asn Pro Val Lys Val Val Asp Ser Gln Ala Pro Gln Gly Arg Gly                  10 15 20 ggg cat atc ggc gga cat atc aaa cgc cgc ccg att cct agg caa acg 211 Gly His Ile Gly Gly His Ile Lys Arg Arg Pro Ile Pro Arg Gln Thr              25 30 35 gaa att tcc gag gtt cgt cga tat atc gtc atg act gcc ctc gca ctc 259 Glu Ile Ser Glu Val Arg Arg Tyr Ile Val Met Thr Ala Leu Ala Leu          40 45 50 ggt ggc ttc gcc atc ggt gtg acg gaa ttt gtc tcc atg ggt ctg ctc 307 Gly Gly Phe Ala Ile Gly Val Thr Glu Phe Val Ser Met Gly Leu Leu      55 60 65 agc gcg atc gcc tcc gac ttt gag atc tcc gaa gac caa gcc gga cac 355 Ser Ala Ile Ala Ser Asp Phe Glu Ile Ser Glu Asp Gln Ala Gly His  70 75 80 85 atc atc acc atc tac gcc ctc gcg 379 Ile Ile Thr Ile Tyr Ala Leu Ala                  90 <210> 138 <211> 93 <212> PRT <213> Corynebacterium glutamicum <400> 138 Met His Glu Ser Gly Lys Asn Pro Val Lys Val Val Asp Ser Gln Ala   1 5 10 15 Pro Gln Gly Arg Gly Gly His Ile Gly Gly His Ile Lys Arg Arg Pro              20 25 30 Ile Pro Arg Gln Thr Glu Ile Ser Glu Val Arg Arg Tyr Ile Val Met          35 40 45 Thr Ala Leu Ala Leu Gly Gly Phe Ala Ile Gly Val Thr Glu Phe Val      50 55 60 Ser Met Gly Leu Leu Ser Ala Ile Ala Ser Asp Phe Glu Ile Ser Glu  65 70 75 80 Asp Gln Ala Gly His Ile Ile Thr Ile Tyr Ala Leu Ala                  85 90 <139> <211> 735 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (712) <223> RXA00109 <400> 139 aagtggggga agatttcgac aactaaccgg gcgcaaagat gaaactaatg cgtccgacca 60 cggcgaaaag gaagtttcgc ccatctatga gaggttgaat gtg gct tca gag aag 115                                             Val Ala Ser Glu Lys                                               1 5 aat cta aaa ttg cgt acc ttg gcg gca gct gct ggg gtg ttg ggc gtt 163 Asn Leu Lys Leu Arg Thr Leu Ala Ala Ala Ala Gly Val Leu Gly Val                  10 15 20 ggc gcg atg tcg atg ctc gtg gct ccg cag gct gct gcc cat gat gtg 211 Gly Ala Met Ser Met Leu Val Ala Pro Gln Ala Ala Ala His Asp Val              25 30 35 gtg gtg gat tct aat cct gaa aat ggc agt gtc gtt gat gag ttc ccg 259 Val Val Asp Ser Asn Pro Glu Asn Gly Ser Val Val Asp Glu Phe Pro          40 45 50 gag acc att gag ttg gag ttt tcc ggt att cct cag gat ctg ttc aca 307 Glu Thr Ile Glu Leu Glu Phe Ser Gly Ile Pro Gln Asp Leu Phe Thr      55 60 65 aca gtt gca ttg agc aat gcg gat tcc gga gag gtg tta act tct gga 355 Thr Val Ala Leu Ser Asn Ala Asp Ser Gly Glu Val Leu Thr Ser Gly  70 75 80 85 act cct cag ctt gag ggg cag cac ttg agc tat gaa gtg cca tct gat 403 Thr Pro Gln Leu Glu Gly Gln His Leu Ser Tyr Glu Val Pro Ser Asp                  90 95 100 gtg cag acg gga gct ggt aac tac att ttg ggt ttc cag atc act tct 451 Val Gln Thr Gly Ala Gly Asn Tyr Ile Leu Gly Phe Gln Ile Thr Ser             105 110 115 tct gat ggt cac gct act aaa ggt tca atc tct ttt gag gtg aca ggc 499 Ser Asp Gly His Ala Thr Lys Gly Ser Ile Ser Phe Glu Val Thr Gly         120 125 130 tct gct gaa acg aca aca gag aca aca gca gag acg aca act gag tca 547 Ser Ala Glu Thr Thr Thr Glu Thr Thr Ala Glu Thr Thr Thr Glu Ser     135 140 145 gca gca acc act gac acc tca gag acc acc gaa gca gag aca act gaa 595 Ala Ala Thr Thr Asp Thr Ser Glu Thr Thr Glu Ala Glu Thr Thr Glu 150 155 160 165 act gct gat gaa act tct gga att cct gcg ccg tgg aat tgg gtt ttg 643 Thr Ala Asp Glu Thr Ser Gly Ile Pro Ala Pro Trp Asn Trp Val Leu                 170 175 180 agc atc gtg gcg gtg ctt gtt gtt gca agt gcc atc gtc atg atg att 691 Ser Ile Val Ala Val Leu Val Val Ala Ser Ala Ile Val Met Met Ile             185 190 195 gca aag aat cgt aac cag aaa taagagggtt tattcaccat gaa 735 Ala Lys Asn Arg Asn Gln Lys         200 <210> 140 <211> 204 <212> PRT <213> Corynebacterium glutamicum <400> 140 Val Ala Ser Glu Lys Asn Leu Lys Leu Arg Thr Leu Ala Ala Ala Ala   1 5 10 15 Gly Val Leu Gly Val Gly Ala Met Ser Met Leu Val Ala Pro Gln Ala              20 25 30 Ala Ala His Asp Val Val Val Asp Ser Asn Pro Glu Asn Gly Ser Val          35 40 45 Val Asp Glu Phe Pro Glu Thr Ile Glu Leu Glu Phe Ser Gly Ile Pro      50 55 60 Gln Asp Leu Phe Thr Thr Val Ala Leu Ser Asn Ala Asp Ser Gly Glu  65 70 75 80 Val Leu Thr Ser Gly Thr Pro Gln Leu Glu Gly Gln His Leu Ser Tyr                  85 90 95 Glu Val Pro Ser Asp Val Gln Thr Gly Ala Gly Asn Tyr Ile Leu Gly             100 105 110 Phe Gln Ile Thr Ser Ser Asp Gly His Ala Thr Lys Gly Ser Ile Ser         115 120 125 Phe Glu Val Thr Gly Ser Ala Glu Thr Thr Thr Glu Thr Thr Ala Glu     130 135 140 Thr Thr Thr Glu Ser Ala Ala Thr Thr Asp Thr Ser Glu Thr Thr Glu 145 150 155 160 Ala Glu Thr Thr Glu Thr Ala Asp Glu Thr Ser Gly Ile Pro Ala Pro                 165 170 175 Trp Asn Trp Val Leu Ser Ile Val Ala Val Leu Val Val Ala Ser Ala             180 185 190 Ile Val Met Met Ile Ala Lys Asn Arg Asn Gln Lys         195 200 <210> 141 <211> 735 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (712) <223> RXA00109 <400> 141 aagtggggga agatttcgac aactaaccgg gcgcaaagat gaaactaatg cgtccgacca 60 cggcgaaaag gaagtttcgc ccatctatga gaggttgaat gtg gct tca gag aag 115                                             Val Ala Ser Glu Lys                                               1 5 aat cta aaa ttg cgt acc ttg gcg gca gct gct ggg gtg ttg ggc gtt 163 Asn Leu Lys Leu Arg Thr Leu Ala Ala Ala Ala Gly Val Leu Gly Val                  10 15 20 ggc gcg atg tcg atg ctc gtg gct ccg cag gct gct gcc cat gat gtg 211 Gly Ala Met Ser Met Leu Val Ala Pro Gln Ala Ala Ala His Asp Val              25 30 35 gtg gtg gat tct aat cct gaa aat ggc agt gtc gtt gat gag ttc ccg 259 Val Val Asp Ser Asn Pro Glu Asn Gly Ser Val Val Asp Glu Phe Pro          40 45 50 gag acc att gag ttg gag ttt tcc ggt att cct cag gat ctg ttc aca 307 Glu Thr Ile Glu Leu Glu Phe Ser Gly Ile Pro Gln Asp Leu Phe Thr      55 60 65 aca gtt gca ttg agc aat gcg gat tcc gga gag gtg tta act tct gga 355 Thr Val Ala Leu Ser Asn Ala Asp Ser Gly Glu Val Leu Thr Ser Gly  70 75 80 85 act cct cag ctt gag ggg cag cac ttg agc tat gaa gtg cca tct gat 403 Thr Pro Gln Leu Glu Gly Gln His Leu Ser Tyr Glu Val Pro Ser Asp                  90 95 100 gtg cag acg gga gct ggt aac tac att ttg ggt ttc cag atc act tct 451 Val Gln Thr Gly Ala Gly Asn Tyr Ile Leu Gly Phe Gln Ile Thr Ser             105 110 115 tct gat ggt cac gct act aaa ggt tca atc tct ttt gag gtg aca ggc 499 Ser Asp Gly His Ala Thr Lys Gly Ser Ile Ser Phe Glu Val Thr Gly         120 125 130 tct gct gaa acg aca aca gag aca aca gca gag acg aca act gag tca 547 Ser Ala Glu Thr Thr Thr Glu Thr Thr Ala Glu Thr Thr Thr Glu Ser     135 140 145 gca gca acc act gac acc tca gag acc acc gaa gca gag aca act gaa 595 Ala Ala Thr Thr Asp Thr Ser Glu Thr Thr Glu Ala Glu Thr Thr Glu 150 155 160 165 act gct gat gaa act tct gga att cct gcg ccg tgg aat tgg gtt ttg 643 Thr Ala Asp Glu Thr Ser Gly Ile Pro Ala Pro Trp Asn Trp Val Leu                 170 175 180 agc atc gtg gcg gtg ctt gtt gtt gca agt gcc atc gtc atg atg att 691 Ser Ile Val Ala Val Leu Val Val Ala Ser Ala Ile Val Met Met Ile             185 190 195 gca aag aat cgt aac cag aaa taagagggtt tattcaccat gaa 735 Ala Lys Asn Arg Asn Gln Lys         200 <210> 142 <211> 204 <212> PRT <213> Corynebacterium glutamicum <400> 142 Val Ala Ser Glu Lys Asn Leu Lys Leu Arg Thr Leu Ala Ala Ala Ala   1 5 10 15 Gly Val Leu Gly Val Gly Ala Met Ser Met Leu Val Ala Pro Gln Ala              20 25 30 Ala Ala His Asp Val Val Val Asp Ser Asn Pro Glu Asn Gly Ser Val          35 40 45 Val Asp Glu Phe Pro Glu Thr Ile Glu Leu Glu Phe Ser Gly Ile Pro      50 55 60 Gln Asp Leu Phe Thr Thr Val Ala Leu Ser Asn Ala Asp Ser Gly Glu  65 70 75 80 Val Leu Thr Ser Gly Thr Pro Gln Leu Glu Gly Gln His Leu Ser Tyr                  85 90 95 Glu Val Pro Ser Asp Val Gln Thr Gly Ala Gly Asn Tyr Ile Leu Gly             100 105 110 Phe Gln Ile Thr Ser Ser Asp Gly His Ala Thr Lys Gly Ser Ile Ser         115 120 125 Phe Glu Val Thr Gly Ser Ala Glu Thr Thr Thr Glu Thr Thr Ala Glu     130 135 140 Thr Thr Thr Glu Ser Ala Ala Thr Thr Asp Thr Ser Glu Thr Thr Glu 145 150 155 160 Ala Glu Thr Thr Glu Thr Ala Asp Glu Thr Ser Gly Ile Pro Ala Pro                 165 170 175 Trp Asn Trp Val Leu Ser Ile Val Ala Val Leu Val Val Ala Ser Ala             180 185 190 Ile Val Met Met Ile Ala Lys Asn Arg Asn Gln Lys         195 200 <210> 143 <211> 864 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (841) <223> RXA00996 <400> 143 atcttaagcc ttttgtccaa ggctctgagt tcttagtggc ggtatttacc aagaagtaac 60 acctctatct tgcacctgat ctggcgtaga ctcataagtt atg agc acc gta acg 115                                             Met Ser Thr Val Thr                                               1 5 gca gtg cag gtc aac ggc cta aaa gtt tcc ata tcg tcc ggt ttt tca 163 Ala Val Gln Val Asn Gly Leu Lys Val Ser Ile Ser Ser Gly Phe Ser                  10 15 20 cgc aag aaa aca aaa acg atc ttg cat gat ctc gat ttc acc gta gag 211 Arg Lys Lys Thr Lys Thr Ile Leu His Asp Leu Asp Phe Thr Val Glu              25 30 35 acc gga aag atc acg gga ttg ctg ggg cca tcg ggc agc ggc aag aca 259 Thr Gly Lys Ile Thr Gly Leu Leu Gly Pro Ser Gly Ser Gly Lys Thr          40 45 50 act ttg atg cgc gcg att gtg gga gtg caa aac ttc gac ggc acc ctt 307 Thr Leu Met Arg Ala Ile Val Gly Val Gln Asn Phe Asp Gly Thr Leu      55 60 65 gag gtg ttt gat cag ccc gca ggt gct gcc tct ctg cgc ggg aaa atc 355 Glu Val Phe Asp Gln Pro Ala Gly Ala Ala Ser Leu Arg Gly Lys Ile  70 75 80 85 ggc tat gtc acc caa aac gcc agc gta tat cac gat ctg tcg gtg ata 403 Gly Tyr Val Thr Gln Asn Ala Ser Val Tyr His Asp Leu Ser Val Ile                  90 95 100 gaa aac ctc aag tat ttc ggg gct ctg gct aaa gga acc tcc act cca 451 Glu Asn Leu Lys Tyr Phe Gly Ala Leu Ala Lys Gly Thr Ser Thr Pro             105 110 115 cgc acc ccg gaa aag att ctg gag gtc tta gac atc gca gat ctt gct 499 Arg Thr Pro Glu Lys Ile Leu Glu Val Leu Asp Ile Ala Asp Leu Ala         120 125 130 caa cgc caa gta tca aca cta tct ggt ggg cag cgc ggc cga gtc tcc 547 Gln Arg Gln Val Ser Thr Leu Ser Gly Gly Gln Arg Gly Arg Val Ser     135 140 145 ctt gga tgt gcg ctt att gcc tca cca gaa ctc ttg gtg atg gat gag 595 Leu Gly Cys Ala Leu Ile Ala Ser Pro Glu Leu Leu Val Met Asp Glu 150 155 160 165 cca acc gtg ggt ttg gat ccc att acc cgg caa gca ctg tgg gaa gag 643 Pro Thr Val Gly Leu Asp Pro Ile Thr Arg Gln Ala Leu Trp Glu Glu                 170 175 180 ttc acc acc atc gca aaa gca ggt gct gga gtg gtt atc tcc agt cac 691 Phe Thr Thr Ile Ala Lys Ala Gly Ala Gly Val Val Ile Ser Ser His             185 190 195 gtg ttg gag gaa gcc gcg cgg tgc gac aac ctc att ttg ttg cgt gat 739 Val Leu Glu Glu Ala Ala Arg Cys Asp Asn Leu Ile Leu Leu Arg Asp         200 205 210 ggt cgg atc atc tgg agg gga aca ccc aca cgc ctt cta gaa gat aca 787 Gly Arg Ile Ile Trp Arg Gly Thr Pro Thr Arg Leu Leu Glu Asp Thr     215 220 225 ggc aaa agc tca tac gaa gat gct ttc ttg gct gcc att gac ggg gta 835 Gly Lys Ser Ser Tyr Glu Asp Ala Phe Leu Ala Ala Ile Asp Gly Val 230 235 240 245 agg tca tgaaccctca ctatctgctt gcc 864 Arg ser          <210> 144 <211> 247 <212> PRT <213> Corynebacterium glutamicum <400> 144 Met Ser Thr Val Thr Ala Val Gln Val Asn Gly Leu Lys Val Ser Ile   1 5 10 15 Ser Ser Gly Phe Ser Arg Lys Lys Thr Lys Thr Ile Leu His Asp Leu              20 25 30 Asp Phe Thr Val Glu Thr Gly Lys Ile Thr Gly Leu Leu Gly Pro Ser          35 40 45 Gly Ser Gly Lys Thr Thr Leu Met Arg Ala Ile Val Gly Val Gln Asn      50 55 60 Phe Asp Gly Thr Leu Glu Val Phe Asp Gln Pro Ala Gly Ala Ala Ser  65 70 75 80 Leu Arg Gly Lys Ile Gly Tyr Val Thr Gln Asn Ala Ser Val Tyr His                  85 90 95 Asp Leu Ser Val Ile Glu Asn Leu Lys Tyr Phe Gly Ala Leu Ala Lys             100 105 110 Gly Thr Ser Thr Pro Arg Thr Pro Glu Lys Ile Leu Glu Val Leu Asp         115 120 125 Ile Ala Asp Leu Ala Gln Arg Gln Val Ser Thr Leu Ser Gly Gly Gln     130 135 140 Arg Gly Arg Val Ser Leu Gly Cys Ala Leu Ile Ala Ser Pro Glu Leu 145 150 155 160 Leu Val Met Asp Glu Pro Thr Val Gly Leu Asp Pro Ile Thr Arg Gln                 165 170 175 Ala Leu Trp Glu Glu Phe Thr Thr Ile Ala Lys Ala Gly Ala Gly Val             180 185 190 Val Ile Ser Ser His Val Leu Glu Glu Ala Ala Arg Cys Asp Asn Leu         195 200 205 Ile Leu Leu Arg Asp Gly Arg Ile Ile Trp Arg Gly Thr Pro Thr Arg     210 215 220 Leu Leu Glu Asp Thr Gly Lys Ser Ser Tyr Glu Asp Ala Phe Leu Ala 225 230 235 240 Ala Ile Asp Gly Val Arg Ser                 245 <210> 145 <211> 2463 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (101) .. (2440) <223> RXN00829 <400> 145 tgttttagcc atggacccca tactagggag agttttgttt tggtgctaga aaaggttcac 60 caagcgcgaa caggcctatg caaacggtac gatatgacac atg caa aaa gct gat 115                                             Met Gln Lys Ala Asp                                               1 5 tcc cat gat tgg att tcg gtc cac ggt gcg aat gaa aac aac ctc aaa 163 Ser His Asp Trp Ile Ser Val His Gly Ala Asn Glu Asn Asn Leu Lys                  10 15 20 aat gtg tcg gtg cgc atc cct aaa agg cgt ctc acc gtg ttc acg ggt 211 Asn Val Ser Val Arg Ile Pro Lys Arg Arg Leu Thr Val Phe Thr Gly              25 30 35 gtg tcg gga tct ggc aag tcc tcg ctg gtg ttc ggc aca att gct gcg 259 Val Ser Gly Ser Gly Lys Ser Ser Leu Val Phe Gly Thr Ile Ala Ala          40 45 50 gaa tca cgc cgg ttg atc aac gaa acc tat agc act ttt gtg caa ggt 307 Glu Ser Arg Arg Leu Ile Asn Glu Thr Tyr Ser Thr Phe Val Gln Gly      55 60 65 ttc atg ccg tcg atg gca agg ccc gat gtt gac cat ttg gaa ggc atc 355 Phe Met Pro Ser Met Ala Arg Pro Asp Val Asp His Leu Glu Gly Ile  70 75 80 85 acc acg gcg atc atc gtc gat cag gag cag atg ggc gca aac cca cgg 403 Thr Thr Ala Ile Ile Val Asp Gln Glu Gln Met Gly Ala Asn Pro Arg                  90 95 100 tct acg gtg ggt acc gca act gat gcc acc gcg atg ttg cgc att ttg 451 Ser Thr Val Gly Thr Ala Thr Asp Ala Thr Ala Met Leu Arg Ile Leu             105 110 115 ttt tcc cga atc gcg gaa cct aac gcg ggt ggc ccg gga gct tat tcc 499 Phe Ser Arg Ile Ala Glu Pro Asn Ala Gly Gly Pro Gly Ala Tyr Ser         120 125 130 ttc aac gtc ccc tct gtt tcc gca tcc ggc gcc atc acg gtg gaa aag 547 Phe Asn Val Pro Ser Val Ser Ala Ser Gly Ala Ile Thr Val Glu Lys     135 140 145 ggc gga aac acc aag cgg gag aaa gct acc ttc aaa cgc acg ggt ggc 595 Gly Gly Asn Thr Lys Arg Glu Lys Ala Thr Phe Lys Arg Thr Gly Gly 150 155 160 165 atg tgc cca gcg tgc gag ggc atg ggc agg gcc tca gac atc gac ctc 643 Met Cys Pro Ala Cys Glu Gly Met Gly Arg Ala Ser Asp Ile Asp Leu                 170 175 180 aaa gag ctt ttc gac gcc tcc ctc tcc ctc aac gac ggc gcc ctg acc 691 Lys Glu Leu Phe Asp Ala Ser Leu Ser Leu Asn Asp Gly Ala Leu Thr             185 190 195 atc ccc ggt tac acc cca ggt gga tgg agt tat cgg atg tat tca gaa 739 Ile Pro Gly Tyr Thr Pro Gly Gly Trp Ser Tyr Arg Met Tyr Ser Glu         200 205 210 tcg ggc ctt ttt gat gct gcc aag ccg att aag gat ttc acc gag gaa 787 Ser Gly Leu Phe Asp Ala Ala Lys Pro Ile Lys Asp Phe Thr Glu Glu     215 220 225 gaa cgc cac aac ttc ctt tat ctt gag ccc acc aag atg aag atc gct 835 Glu Arg His Asn Phe Leu Tyr Leu Glu Pro Thr Lys Met Lys Ile Ala 230 235 240 245 ggc atc aac atg acc tat gag ggt ctt atc ccc cgc att cag aaa tcc 883 Gly Ile Asn Met Thr Tyr Glu Gly Leu Ile Pro Arg Ile Gln Lys Ser                 250 255 260 atg ttg tct aag gat cgc gaa ggc atg cag aag cat att cgt gcg ttc 931 Met Leu Ser Lys Asp Arg Glu Gly Met Gln Lys His Ile Arg Ala Phe             265 270 275 gtg gat cga gcg gtt acc ttc att cct tgc cct gcg tgt ggt gga act 979 Val Asp Arg Ala Val Thr Phe Ile Pro Cys Pro Ala Cys Gly Gly Thr         280 285 290 cga tta gcg cca cat gcc ttg gag tcc aag atc aat ggc aaa aac atc 1027 Arg Leu Ala Pro His Ala Leu Glu Ser Lys Ile Asn Gly Lys Asn Ile     295 300 305 gct gag ttg tgc gcg atg gag gtc cgt gat ttg gcc aag tgg atc aaa 1075 Ala Glu Leu Cys Ala Met Glu Val Arg Asp Leu Ala Lys Trp Ile Lys 310 315 320 325 acg gtg gaa gcc ccc tcg gtt gct ccc ctg ctc acc gca ctg act gaa 1123 Thr Val Glu Ala Pro Ser Val Ala Pro Leu Leu Thr Ala Leu Thr Glu                 330 335 340 acc ctg gat aac ttc gtg gag atc ggt ttg ggc tat atc caa ctc gat 1171 Thr Leu Asp Asn Phe Val Glu Ile Gly Leu Gly Tyr Ile Gln Leu Asp             345 350 355 cgc ccc gct ggc acg ttg tct ggt ggt gag gca cag cgc acc aag atg 1219 Arg Pro Ala Gly Thr Leu Ser Gly Gly Glu Ala Gln Arg Thr Lys Met         360 365 370 atc cgc cat ttg ggc tct gca ttg act gac gtc acc tat gtt ttt gat 1267 Ile Arg His Leu Gly Ser Ala Leu Thr Asp Val Thr Tyr Val Phe Asp     375 380 385 gaa ccc acc gcc ggt ttg cac gcc tac gac att gaa cgc atg aac aag 1315 Glu Pro Thr Ala Gly Leu His Ala Tyr Asp Ile Glu Arg Met Asn Lys 390 395 400 405 ttg ctg ctc gat ctt cgc gat aaa ggc aat acc gtt tta gtc gtg gag 1363 Leu Leu Leu Asp Leu Arg Asp Lys Gly Asn Thr Val Leu Val Val Glu                 410 415 420 cac aag ccg gaa acc atc gcc att gca gat cat gtg gtg gac ctt ggg 1411 His Lys Pro Glu Thr Ile Ala Ile Ala Asp His Val Val Asp Leu Gly             425 430 435 cca ggt gca ggc gcg ggt gga ggt gaa att cgg ttt gag ggg agc gtc 1459 Pro Gly Ala Gly Ala Gly Gly Gly Glu Ile Arg Phe Glu Gly Ser Val         440 445 450 gac aag ctt aaa gac agc gac acc gtg act ggc ctc cat ttt aat gac 1507 Asp Lys Leu Lys Asp Ser Asp Thr Val Thr Gly Leu His Phe Asn Asp     455 460 465 cgg gcg tca ttg aag gaa tcc gtg cgt gcg ccg cat ggc gcc ctg gag 1555 Arg Ala Ser Leu Lys Glu Ser Val Arg Ala Pro His Gly Ala Leu Glu 470 475 480 485 atc cgc ggg gcc gat cga aat aat ttg aac aat gtg gat gtc gat att 1603 Ile Arg Gly Ala Asp Arg Asn Asn Leu Asn Asn Val Asp Val Asp Ile                 490 495 500 ccg ctc ggc gtg ttc acg gcg att tcc ggc gtt gca ggt tcg ggt aag 1651 Pro Leu Gly Val Phe Thr Ala Ile Ser Gly Val Ala Gly Ser Gly Lys             505 510 515 tcc tcg ttg att cat gag att ccg cgt gat gag tcg gtt gtg ttt gtc 1699 Ser Ser Leu Ile His Glu Ile Pro Arg Asp Glu Ser Val Val Phe Val         520 525 530 gat caa acc gca atc cac ggt tct aat cgt tcc aat cct gcg aca tat 1747 Asp Gln Thr Ala Ile His Gly Ser Asn Arg Ser Asn Pro Ala Thr Tyr     535 540 545 aca ggc atg ctg gat tcg att cgc aag gct ttt gcc aag gcc aat gat 1795 Thr Gly Met Leu Asp Ser Ile Arg Lys Ala Phe Ala Lys Ala Asn Asp 550 555 560 565 gtg aaa ccg gcg ctg ttc tcc ccc aat tct gaa ggc gcg tgc cca aac 1843 Val Lys Pro Ala Leu Phe Ser Pro Asn Ser Glu Gly Ala Cys Pro Asn                 570 575 580 tgt aag ggc gcc ggc tcg gtc tat gtc gat ttg ggc atg atg gct ggg 1891 Cys Lys Gly Ala Gly Ser Val Tyr Val Asp Leu Gly Met Met Ala Gly             585 590 595 gta tct tcg ccg tgt gag gtg tgc gag ggc aag cgt ttt gat gag tcc 1939 Val Ser Ser Pro Cys Glu Val Cys Glu Gly Lys Arg Phe Asp Glu Ser         600 605 610 gtg ttg gac tac cac ttt ggt ggc aag gac atc gca gac gtg ttg ggg 1987 Val Leu Asp Tyr His Phe Gly Gly Lys Asp Ile Ala Asp Val Leu Gly     615 620 625 ctg tcg gct gcc aat gcg tat gag ttt ttc gcg gcg aaa gat tca aag 2035 Leu Ser Ala Ala Asn Ala Tyr Glu Phe Phe Ala Ala Lys Asp Ser Lys 630 635 640 645 att ttg cct gcg gca aag atc gca aag agg ctt gtc gac gtc ggc ctc 2083 Ile Leu Pro Ala Ala Lys Ile Ala Lys Arg Leu Val Asp Val Gly Leu                 650 655 660 ggc tac atc acc ctc ggc cag ccg ctc acc acg ttg tcc ggc ggt gaa 2131 Gly Tyr Ile Thr Leu Gly Gln Pro Leu Thr Thr Leu Ser Gly Gly Glu             665 670 675 cgc cag cgt ttg aag ctc gcc acc cac atg gca gac aag gcc acc acc 2179 Arg Gln Arg Leu Lys Leu Ala Thr His Met Ala Asp Lys Ala Thr Thr         680 685 690 ttt att ttg gat gag ccc acc aca ggc ctg cac ctc gct gat gtg aaa 2227 Phe Ile Leu Asp Glu Pro Thr Thr Gly Leu His Leu Ala Asp Val Lys     695 700 705 acc ttg ctg gat ctt ttt gat caa ctg gtt gat gac ggc aag tct gtc 2275 Thr Leu Leu Asp Leu Phe Asp Gln Leu Val Asp Asp Gly Lys Ser Val 710 715 720 725 atc gtc atc gaa cac cac ctc ggc gtg ctc gct cac gct gac cac atc 2323 Ile Val Ile Glu His His Leu Gly Val Leu Ala His Ala Asp His Ile                 730 735 740 att gat gtc ggc cct ggt gca ggt tct gat ggt ggc tcg att gta ttc 2371 Ile Asp Val Gly Pro Gly Ala Gly Ser Asp Gly Gly Ser Ile Val Phe             745 750 755 gag ggc agc ccc gcg gaa ctc atc aaa act gat act cca aca gga cgc 2419 Glu Gly Ser Pro Ala Glu Leu Ile Lys Thr Asp Thr Pro Thr Gly Arg         760 765 770 cac ctt aaa gct tat gta gat tagtttctta tggaaaaccc tgg 2463 His Leu Lys Ala Tyr Val Asp     775 780 <210> 146 <211> 780 <212> PRT <213> Corynebacterium glutamicum <400> 146 Met Gln Lys Ala Asp Ser His Asp Trp Ile Ser Val His Gly Ala Asn   1 5 10 15 Glu Asn Asn Leu Lys Asn Val Ser Val Arg Ile Pro Lys Arg Arg Leu              20 25 30 Thr Val Phe Thr Gly Val Ser Gly Ser Gly Lys Ser Ser Leu Val Phe          35 40 45 Gly Thr Ile Ala Ala Glu Ser Arg Arg Leu Ile Asn Glu Thr Tyr Ser      50 55 60 Thr Phe Val Gln Gly Phe Met Pro Ser Met Ala Arg Pro Asp Val Asp  65 70 75 80 His Leu Glu Gly Ile Thr Thr Ala Ile Ile Val Asp Gln Glu Gln Met                  85 90 95 Gly Ala Asn Pro Arg Ser Thr Val Gly Thr Ala Thr Asp Ala Thr Ala             100 105 110 Met Leu Arg Ile Leu Phe Ser Arg Ile Ala Glu Pro Asn Ala Gly Gly         115 120 125 Pro Gly Ala Tyr Ser Phe Asn Val Pro Ser Val Ser Ala Ser Gly Ala     130 135 140 Ile Thr Val Glu Lys Gly Gly Asn Thr Lys Arg Glu Lys Ala Thr Phe 145 150 155 160 Lys Arg Thr Gly Gly Met Cys Pro Ala Cys Glu Gly Met Gly Arg Ala                 165 170 175 Ser Asp Ile Asp Leu Lys Glu Leu Phe Asp Ala Ser Leu Ser Leu Asn             180 185 190 Asp Gly Ala Leu Thr Ile Pro Gly Tyr Thr Pro Gly Gly Trp Ser Tyr         195 200 205 Arg Met Tyr Ser Glu Ser Gly Leu Phe Asp Ala Ala Lys Pro Ile Lys     210 215 220 Asp Phe Thr Glu Glu Glu Arg His Asn Phe Leu Tyr Leu Glu Pro Thr 225 230 235 240 Lys Met Lys Ile Ala Gly Ile Asn Met Thr Tyr Glu Gly Leu Ile Pro                 245 250 255 Arg Ile Gln Lys Ser Met Leu Ser Lys Asp Arg Glu Gly Met Gln Lys             260 265 270 His Ile Arg Ala Phe Val Asp Arg Ala Val Thr Phe Ile Pro Cys Pro         275 280 285 Ala Cys Gly Gly Thr Arg Leu Ala Pro His Ala Leu Glu Ser Lys Ile     290 295 300 Asn Gly Lys Asn Ile Ala Glu Leu Cys Ala Met Glu Val Arg Asp Leu 305 310 315 320 Ala Lys Trp Ile Lys Thr Val Glu Ala Pro Ser Val Ala Pro Leu Leu                 325 330 335 Thr Ala Leu Thr Glu Thr Leu Asp Asn Phe Val Glu Ile Gly Leu Gly             340 345 350 Tyr Ile Gln Leu Asp Arg Pro Ala Gly Thr Leu Ser Gly Gly Glu Ala         355 360 365 Gln Arg Thr Lys Met Ile Arg His Leu Gly Ser Ala Leu Thr Asp Val     370 375 380 Thr Tyr Val Phe Asp Glu Pro Thr Ala Gly Leu His Ala Tyr Asp Ile 385 390 395 400 Glu Arg Met Asn Lys Leu Leu Leu Asp Leu Arg Asp Lys Gly Asn Thr                 405 410 415 Val Leu Val Val Glu His Lys Pro Glu Thr Ile Ala Ile Ala Asp His             420 425 430 Val Val Asp Leu Gly Pro Gly Ala Gly Ala Gly Gly Gly Glu Ile Arg         435 440 445 Phe Glu Gly Ser Val Asp Lys Leu Lys Asp Ser Asp Thr Val Thr Gly     450 455 460 Leu His Phe Asn Asp Arg Ala Ser Leu Lys Glu Ser Val Arg Ala Pro 465 470 475 480 His Gly Ala Leu Glu Ile Arg Gly Ala Asp Arg Asn Asn Leu Asn Asn                 485 490 495 Val Asp Val Asp Ile Pro Leu Gly Val Phe Thr Ala Ile Ser Gly Val             500 505 510 Ala Gly Ser Gly Lys Ser Ser Leu Ile His Glu Ile Pro Arg Asp Glu         515 520 525 Ser Val Val Phe Val Asp Gln Thr Ala Ile His Gly Ser Asn Arg Ser     530 535 540 Asn Pro Ala Thr Tyr Thr Gly Met Leu Asp Ser Ile Arg Lys Ala Phe 545 550 555 560 Ala Lys Ala Asn Asp Val Lys Pro Ala Leu Phe Ser Pro Asn Ser Glu                 565 570 575 Gly Ala Cys Pro Asn Cys Lys Gly Ala Gly Ser Val Tyr Val Asp Leu             580 585 590 Gly Met Met Ala Gly Val Ser Ser Pro Cys Glu Val Cys Glu Gly Lys         595 600 605 Arg Phe Asp Glu Ser Val Leu Asp Tyr His Phe Gly Gly Lys Asp Ile     610 615 620 Ala Asp Val Leu Gly Leu Ser Ala Ala Asn Ala Tyr Glu Phe Phe Ala 625 630 635 640 Ala Lys Asp Ser Lys Ile Leu Pro Ala Ala Lys Ile Ala Lys Arg Leu                 645 650 655 Val Asp Val Gly Leu Gly Tyr Ile Thr Leu Gly Gln Pro Leu Thr Thr             660 665 670 Leu Ser Gly Gly Glu Arg Gln Arg Leu Lys Leu Ala Thr His Met Ala         675 680 685 Asp Lys Ala Thr Thr Phe Ile Leu Asp Glu Pro Thr Thr Gly Leu His     690 695 700 Leu Ala Asp Val Lys Thr Leu Leu Asp Leu Phe Asp Gln Leu Val Asp 705 710 715 720 Asp Gly Lys Ser Val Ile Val Ile Glu His His Leu Gly Val Leu Ala                 725 730 735 His Ala Asp His Ile Ile Asp Val Gly Pro Gly Ala Gly Ser Asp Gly             740 745 750 Gly Ser Ile Val Phe Glu Gly Ser Pro Ala Glu Leu Ile Lys Thr Asp         755 760 765 Thr Pro Thr Gly Arg His Leu Lys Ala Tyr Val Asp     770 775 780 <210> 147 <211> 278 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (1) .. (255) <223> FRXA00829 <400> 147 ttg gat gag ccc acc aca ggc ctg cac ctc gct gat gtg aaa acc ttg 48 Leu Asp Glu Pro Thr Thr Gly Leu His Leu Ala Asp Val Lys Thr Leu   1 5 10 15 ctg gat ctt ttt gat caa ctg gtt gat gac ggc aag tct gtc atc gtc 96 Leu Asp Leu Phe Asp Gln Leu Val Asp Asp Gly Lys Ser Val Ile Val              20 25 30 atc gaa cac cac ctc ggc gtg ctc gct cac gct gac cac atc att gat 144 Ile Glu His His Leu Gly Val Leu Ala His Ala Asp His Ile Ile Asp          35 40 45 gtc ggc cct ggt gca ggt tct gat ggt ggc tcg att gta ttc gag ggc 192 Val Gly Pro Gly Ala Gly Ser Asp Gly Gly Ser Ile Val Phe Glu Gly      50 55 60 agc ccc gcg gaa ctc atc aaa act gat act cca aca gga cgc cac ctt 240 Ser Pro Ala Glu Leu Ile Lys Thr Asp Thr Pro Thr Gly Arg His Leu  65 70 75 80 aaa gct tat gta gat tagtttctta tggaaaaccc tgg 278 Lys Ala Tyr Val Asp                  85 <210> 148 <211> 85 <212> PRT <213> Corynebacterium glutamicum <400> 148 Leu Asp Glu Pro Thr Thr Gly Leu His Leu Ala Asp Val Lys Thr Leu   1 5 10 15 Leu Asp Leu Phe Asp Gln Leu Val Asp Asp Gly Lys Ser Val Ile Val              20 25 30 Ile Glu His His Leu Gly Val Leu Ala His Ala Asp His Ile Ile Asp          35 40 45 Val Gly Pro Gly Ala Gly Ser Asp Gly Gly Ser Ile Val Phe Glu Gly      50 55 60 Ser Pro Ala Glu Leu Ile Lys Thr Asp Thr Pro Thr Gly Arg His Leu  65 70 75 80 Lys Ala Tyr Val Asp                  85 <210> 149 <211> 1663 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1663) <223> FRXA00834 <400> 149 tgttttagcc atggacccca tactagggag agttttgttt tggtgctaga aaaggttcac 60 caagcgcgaa caggcctatg caaacggtac gatatgacac atg caa aaa gct gat 115                                             Met Gln Lys Ala Asp                                               1 5 tcc cat gat tgg att tcg gtc cac ggt gcg aat gaa aac aac ctc aaa 163 Ser His Asp Trp Ile Ser Val His Gly Ala Asn Glu Asn Asn Leu Lys                  10 15 20 aat gtg tcg gtg cgc atc cct aaa agg cgt ctc acc gtg ttc acg ggt 211 Asn Val Ser Val Arg Ile Pro Lys Arg Arg Leu Thr Val Phe Thr Gly              25 30 35 gtg tcg gga tct ggc aag tcc tcg ctg gtg ttc ggc aca att gct gcg 259 Val Ser Gly Ser Gly Lys Ser Ser Leu Val Phe Gly Thr Ile Ala Ala          40 45 50 gaa tca cgc cgg ttg atc aac gaa acc tat agc act ttt gtg caa ggt 307 Glu Ser Arg Arg Leu Ile Asn Glu Thr Tyr Ser Thr Phe Val Gln Gly      55 60 65 ttc atg ccg tcg atg gca agg ccc gat gtt gac cat ttg gaa ggc atc 355 Phe Met Pro Ser Met Ala Arg Pro Asp Val Asp His Leu Glu Gly Ile  70 75 80 85 acc acg gcg atc atc gtc gat cag gag cag atg ggc gca aac cca cgg 403 Thr Thr Ala Ile Ile Val Asp Gln Glu Gln Met Gly Ala Asn Pro Arg                  90 95 100 tct acg gtg ggt acc gca act gat gcc acc gcg atg ttg cgc att ttg 451 Ser Thr Val Gly Thr Ala Thr Asp Ala Thr Ala Met Leu Arg Ile Leu             105 110 115 ttt tcc cga atc gcg gaa cct aac gcg ggt ggc ccg gga gct tat tcc 499 Phe Ser Arg Ile Ala Glu Pro Asn Ala Gly Gly Pro Gly Ala Tyr Ser         120 125 130 ttc aac gtc ccc tct gtt tcc gca tcc ggc gcc atc acg gtg gaa aag 547 Phe Asn Val Pro Ser Val Ser Ala Ser Gly Ala Ile Thr Val Glu Lys     135 140 145 ggc gga aac acc aag cgg gag aaa gct acc ttc aaa cgc acg ggt ggc 595 Gly Gly Asn Thr Lys Arg Glu Lys Ala Thr Phe Lys Arg Thr Gly Gly 150 155 160 165 atg tgc cca gcg tgc gag ggc atg ggc agg gcc tca gac atc gac ctc 643 Met Cys Pro Ala Cys Glu Gly Met Gly Arg Ala Ser Asp Ile Asp Leu                 170 175 180 aaa gag ctt ttc gac gcc tcc ctc tcc ctc aac gac ggc gcc ctg acc 691 Lys Glu Leu Phe Asp Ala Ser Leu Ser Leu Asn Asp Gly Ala Leu Thr             185 190 195 atc ccc ggt tac acc cca ggt gga tgg agt tat cgg atg tat tca gaa 739 Ile Pro Gly Tyr Thr Pro Gly Gly Trp Ser Tyr Arg Met Tyr Ser Glu         200 205 210 tcg ggc ctt ttt gat gct gcc aag ccg att aag gat ttc acc gag gaa 787 Ser Gly Leu Phe Asp Ala Ala Lys Pro Ile Lys Asp Phe Thr Glu Glu     215 220 225 gaa cgc cac aac ttc ctt tat ctt gag ccc acc aag atg aag atc gct 835 Glu Arg His Asn Phe Leu Tyr Leu Glu Pro Thr Lys Met Lys Ile Ala 230 235 240 245 ggc atc aac atg acc tat gag ggt ctt atc ccc cgc att cag aaa tcc 883 Gly Ile Asn Met Thr Tyr Glu Gly Leu Ile Pro Arg Ile Gln Lys Ser                 250 255 260 atg ttg tct aag gat cgc gaa ggc atg cag aag cat att cgt gcg ttc 931 Met Leu Ser Lys Asp Arg Glu Gly Met Gln Lys His Ile Arg Ala Phe             265 270 275 gtg gat cga gcg gtt acc ttc att cct tgc cct gcg tgt ggt gga act 979 Val Asp Arg Ala Val Thr Phe Ile Pro Cys Pro Ala Cys Gly Gly Thr         280 285 290 cga tta gcg cca cat gcc ttg gag tcc aag atc aat ggc aaa aac atc 1027 Arg Leu Ala Pro His Ala Leu Glu Ser Lys Ile Asn Gly Lys Asn Ile     295 300 305 gct gag ttg tgc gcg atg gag gtc cgt gat ttg gcc aag tgg atc aaa 1075 Ala Glu Leu Cys Ala Met Glu Val Arg Asp Leu Ala Lys Trp Ile Lys 310 315 320 325 acg gtg gaa gcc ccc tcg gtt gct ccc ctg ctc acc gca ctg act gaa 1123 Thr Val Glu Ala Pro Ser Val Ala Pro Leu Leu Thr Ala Leu Thr Glu                 330 335 340 acc ctg gat aac ttc gtg gag atc ggt ttg ggc tat atc caa ctc gat 1171 Thr Leu Asp Asn Phe Val Glu Ile Gly Leu Gly Tyr Ile Gln Leu Asp             345 350 355 cgc ccc gct ggc acg ttg tct ggt ggt gag gca cag cgc acc aag atg 1219 Arg Pro Ala Gly Thr Leu Ser Gly Gly Glu Ala Gln Arg Thr Lys Met         360 365 370 atc cgc cat ttg ggc tct gca ttg act gac gtc acc tat gtt ttt gat 1267 Ile Arg His Leu Gly Ser Ala Leu Thr Asp Val Thr Tyr Val Phe Asp     375 380 385 gaa ccc acc gcc ggt ttg cac gcc tac gac att gaa cgc atg aac aag 1315 Glu Pro Thr Ala Gly Leu His Ala Tyr Asp Ile Glu Arg Met Asn Lys 390 395 400 405 ttg ctg ctc gat ctt cgc gat aaa ggc aat acc gtt tta gtc gtg gag 1363 Leu Leu Leu Asp Leu Arg Asp Lys Gly Asn Thr Val Leu Val Val Glu                 410 415 420 cac aag ccg gaa acc atc gcc att gca gat cat gtg gtg gac ctt ggg 1411 His Lys Pro Glu Thr Ile Ala Ile Ala Asp His Val Val Asp Leu Gly             425 430 435 cca ggt gca ggc gcg ggt gga ggt gaa att cgg ttt gag ggg agc gtc 1459 Pro Gly Ala Gly Ala Gly Gly Gly Glu Ile Arg Phe Glu Gly Ser Val         440 445 450 gac aag ctt aaa gac agc gac acc gtg act ggc ctc cat ttt aat gac 1507 Asp Lys Leu Lys Asp Ser Asp Thr Val Thr Gly Leu His Phe Asn Asp     455 460 465 cgg gcg tca ttg aag gaa tcc gtg cgt gcg ccg cat ggc gcc ctg gag 1555 Arg Ala Ser Leu Lys Glu Ser Val Arg Ala Pro His Gly Ala Leu Glu 470 475 480 485 atc cgc ggg gcc gat cga aat aat ttg aac aat gtg gat gtc gat att 1603 Ile Arg Gly Ala Asp Arg Asn Asn Leu Asn Asn Val Asp Val Asp Ile                 490 495 500 ccg ctc ggc gtg ttc acg gcg att tcc ggc gtt gca ggt tcg ggt aag 1651 Pro Leu Gly Val Phe Thr Ala Ile Ser Gly Val Ala Gly Ser Gly Lys             505 510 515 tcc tcg ttg att 1663 Ser Ser Leu Ile         520 <210> 150 <211> 521 <212> PRT <213> Corynebacterium glutamicum <400> 150 Met Gln Lys Ala Asp Ser His Asp Trp Ile Ser Val His Gly Ala Asn   1 5 10 15 Glu Asn Asn Leu Lys Asn Val Ser Val Arg Ile Pro Lys Arg Arg Leu              20 25 30 Thr Val Phe Thr Gly Val Ser Gly Ser Gly Lys Ser Ser Leu Val Phe          35 40 45 Gly Thr Ile Ala Ala Glu Ser Arg Arg Leu Ile Asn Glu Thr Tyr Ser      50 55 60 Thr Phe Val Gln Gly Phe Met Pro Ser Met Ala Arg Pro Asp Val Asp  65 70 75 80 His Leu Glu Gly Ile Thr Thr Ala Ile Ile Val Asp Gln Glu Gln Met                  85 90 95 Gly Ala Asn Pro Arg Ser Thr Val Gly Thr Ala Thr Asp Ala Thr Ala             100 105 110 Met Leu Arg Ile Leu Phe Ser Arg Ile Ala Glu Pro Asn Ala Gly Gly         115 120 125 Pro Gly Ala Tyr Ser Phe Asn Val Pro Ser Val Ser Ala Ser Gly Ala     130 135 140 Ile Thr Val Glu Lys Gly Gly Asn Thr Lys Arg Glu Lys Ala Thr Phe 145 150 155 160 Lys Arg Thr Gly Gly Met Cys Pro Ala Cys Glu Gly Met Gly Arg Ala                 165 170 175 Ser Asp Ile Asp Leu Lys Glu Leu Phe Asp Ala Ser Leu Ser Leu Asn             180 185 190 Asp Gly Ala Leu Thr Ile Pro Gly Tyr Thr Pro Gly Gly Trp Ser Tyr         195 200 205 Arg Met Tyr Ser Glu Ser Gly Leu Phe Asp Ala Ala Lys Pro Ile Lys     210 215 220 Asp Phe Thr Glu Glu Glu Arg His Asn Phe Leu Tyr Leu Glu Pro Thr 225 230 235 240 Lys Met Lys Ile Ala Gly Ile Asn Met Thr Tyr Glu Gly Leu Ile Pro                 245 250 255 Arg Ile Gln Lys Ser Met Leu Ser Lys Asp Arg Glu Gly Met Gln Lys             260 265 270 His Ile Arg Ala Phe Val Asp Arg Ala Val Thr Phe Ile Pro Cys Pro         275 280 285 Ala Cys Gly Gly Thr Arg Leu Ala Pro His Ala Leu Glu Ser Lys Ile     290 295 300 Asn Gly Lys Asn Ile Ala Glu Leu Cys Ala Met Glu Val Arg Asp Leu 305 310 315 320 Ala Lys Trp Ile Lys Thr Val Glu Ala Pro Ser Val Ala Pro Leu Leu                 325 330 335 Thr Ala Leu Thr Glu Thr Leu Asp Asn Phe Val Glu Ile Gly Leu Gly             340 345 350 Tyr Ile Gln Leu Asp Arg Pro Ala Gly Thr Leu Ser Gly Gly Glu Ala         355 360 365 Gln Arg Thr Lys Met Ile Arg His Leu Gly Ser Ala Leu Thr Asp Val     370 375 380 Thr Tyr Val Phe Asp Glu Pro Thr Ala Gly Leu His Ala Tyr Asp Ile 385 390 395 400 Glu Arg Met Asn Lys Leu Leu Leu Asp Leu Arg Asp Lys Gly Asn Thr                 405 410 415 Val Leu Val Val Glu His Lys Pro Glu Thr Ile Ala Ile Ala Asp His             420 425 430 Val Val Asp Leu Gly Pro Gly Ala Gly Ala Gly Gly Gly Glu Ile Arg         435 440 445 Phe Glu Gly Ser Val Asp Lys Leu Lys Asp Ser Asp Thr Val Thr Gly     450 455 460 Leu His Phe Asn Asp Arg Ala Ser Leu Lys Glu Ser Val Arg Ala Pro 465 470 475 480 His Gly Ala Leu Glu Ile Arg Gly Ala Asp Arg Asn Asn Leu Asn Asn                 485 490 495 Val Asp Val Asp Ile Pro Leu Gly Val Phe Thr Ala Ile Ser Gly Val             500 505 510 Ala Gly Ser Gly Lys Ser Ser Leu Ile         515 520 <210> 151 <211> 864 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (841) <223> RXA00995 <400> 151 gtcggatcat ctggagggga acacccacac gccttctaga agatacaggc aaaagctcat 60 acgaagatgc tttcttggct gccattgacg gggtaaggtc atg aac cct cac tat 115                                             Met Asn Pro His Tyr                                               1 5 ctg ctt gcc acg gtc aaa cga gtc ctg ctg cag ctg aaa gcc gat aaa 163 Leu Leu Ala Thr Val Lys Arg Val Leu Leu Gln Leu Lys Ala Asp Lys                  10 15 20 cgt tcc atc gcg ctg att ctt cta gca ccc gtg gcg ttg atg tcg ctg 211 Arg Ser Ile Ala Leu Ile Leu Leu Ala Pro Val Ala Leu Met Ser Leu              25 30 35 ttt tat tac atg tat tcc tcc aca ccg gca ggc acc cag ctg ttt aag 259 Phe Tyr Tyr Met Tyr Ser Ser Thr Pro Ala Gly Thr Gln Leu Phe Lys          40 45 50 acc att tcc acg gtc atg atc gca gtg ttc ccc ttg atg ctc atg ttt 307 Thr Ile Ser Thr Val Met Ile Ala Val Phe Pro Leu Met Leu Met Phe      55 60 65 ttg atg acg tcg gtg acg atg caa aga gaa cgc aac gct gga acg ctc 355 Leu Met Thr Ser Val Thr Met Gln Arg Glu Arg Asn Ala Gly Thr Leu  70 75 80 85 gag cgc ttg tgg acc acg aac att cac cgc gtt gat ttg atc ggt ggc 403 Glu Arg Leu Trp Thr Thr Asn Ile His Arg Val Asp Leu Ile Gly Gly                  90 95 100 tac ggg gtg gcc ttc ggc atc atg gcg gtg gcg caa tct ttg ctc atg 451 Tyr Gly Val Ala Phe Gly Ile Met Ala Val Ala Gln Ser Leu Leu Met             105 110 115 gtg ctc acc ctt cgg tat ctc ctg ggt gtg gaa acc gaa tcg gag tgg 499 Val Leu Thr Leu Arg Tyr Leu Leu Gly Val Glu Thr Glu Ser Glu Trp         120 125 130 tgg att tct acg ctc att gct gcg atc acc ggt ctt atc gga gtg tct 547 Trp Ile Ser Thr Leu Ile Ala Ala Ile Thr Gly Leu Ile Gly Val Ser     135 140 145 ctt ggc ctg ttg agc tct gcg ttt gcc agc act gag ttc caa gct atc 595 Leu Gly Leu Leu Ser Ser Ala Phe Ala Ser Thr Glu Phe Gln Ala Ile 150 155 160 165 caa acg ctg ccg ttg ctt att ttg ccc cag ttc cta ttg tgc ggt ttg 643 Gln Thr Leu Pro Leu Leu Ile Leu Pro Gln Phe Leu Leu Cys Gly Leu                 170 175 180 ctg atc cca cgg gat gat ctg ccg gat gtg ttg cgc tgg gtt tct aat 691 Leu Ile Pro Arg Asp Asp Leu Pro Asp Val Leu Arg Trp Val Ser Asn             185 190 195 gtg ttg ccg ctg tcc tat gca gtt gat gca gcg ctt gag gcc tca cgg 739 Val Leu Pro Leu Ser Tyr Ala Val Asp Ala Ala Leu Glu Ala Ser Arg         200 205 210 acg gga atc gga cag caa gta gtg gtc aac att gcc atc tgc gcc gcg 787 Thr Gly Ile Gly Gln Gln Val Val Val Asn Ile Ala Ile Cys Ala Ala     215 220 225 ttt gcc gtg agc ttc ctg ctg gtg gcg gcg cta tcg atg ccg aga atg 835 Phe Ala Val Ser Phe Leu Leu Val Ala Ala Leu Ser Met Pro Arg Met 230 235 240 245 acc cgc tagattactc ttccagcgag gtg 864 Thr arg          <210> 152 <211> 247 <212> PRT <213> Corynebacterium glutamicum <400> 152 Met Asn Pro His Tyr Leu Leu Ala Thr Val Lys Arg Val Leu Leu Gln   1 5 10 15 Leu Lys Ala Asp Lys Arg Ser Ile Ala Leu Ile Leu Leu Ala Pro Val              20 25 30 Ala Leu Met Ser Leu Phe Tyr Tyr Met Tyr Ser Ser Thr Pro Ala Gly          35 40 45 Thr Gln Leu Phe Lys Thr Ile Ser Thr Val Met Ile Ala Val Phe Pro      50 55 60 Leu Met Leu Met Phe Leu Met Thr Ser Val Thr Met Gln Arg Glu Arg  65 70 75 80 Asn Ala Gly Thr Leu Glu Arg Leu Trp Thr Thr Asn Ile His Arg Val                  85 90 95 Asp Leu Ile Gly Gly Tyr Gly Val Ala Phe Gly Ile Met Ala Val Ala             100 105 110 Gln Ser Leu Leu Met Val Leu Thr Leu Arg Tyr Leu Leu Gly Val Glu         115 120 125 Thr Glu Ser Glu Trp Trp Ile Ser Thr Leu Ile Ala Ala Ile Thr Gly     130 135 140 Leu Ile Gly Val Ser Leu Gly Leu Leu Ser Ser Ala Phe Ala Ser Thr 145 150 155 160 Glu Phe Gln Ala Ile Gln Thr Leu Pro Leu Leu Ile Leu Pro Gln Phe                 165 170 175 Leu Leu Cys Gly Leu Leu Ile Pro Arg Asp Asp Leu Pro Asp Val Leu             180 185 190 Arg Trp Val Ser Asn Val Leu Pro Leu Ser Tyr Ala Val Asp Ala Ala         195 200 205 Leu Glu Ala Ser Arg Thr Gly Ile Gly Gln Gln Val Val Val Asn Ile     210 215 220 Ala Ile Cys Ala Ala Phe Ala Val Ser Phe Leu Leu Val Ala Ala Leu 225 230 235 240 Ser Met Pro Arg Met Thr Arg                 245 <210> 153 <211> 1353 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1330) <223> RXN00803 <400> 153 tcatccttcc ttagctcgcg tgagcttccc aagcgtaagc acccccgtgt gagggcataa 60 cggccgttct gttaaagatt ggtctggcca tttcctccat atg ggg gtg tcc gcg 115                                             Met Gly Val Ser Ala                                               1 5 ctt aac atg tct gac atg gtg gcg aac aaa cgg gca cag cgt aaa gtc 163 Leu Asn Met Ser Asp Met Val Ala Asn Lys Arg Ala Gln Arg Lys Val                  10 15 20 tgg cta gcg gta gct tta tcg gtc ttt acg gtc gcg tgg ggt ggc aat 211 Trp Leu Ala Val Ala Leu Ser Val Phe Thr Val Ala Trp Gly Gly Asn              25 30 35 gaa ttc act ccc ttg ctg gtg ttt tac cga ggt gaa ggg ttc ttt agc 259 Glu Phe Thr Pro Leu Leu Val Phe Tyr Arg Gly Glu Gly Phe Phe Ser          40 45 50 aac ctg ttc atc gac ctt ttg ctg gtg ttt tat gcc atc gga gta gcg 307 Asn Leu Phe Ile Asp Leu Leu Leu Val Phe Tyr Ala Ile Gly Val Ala      55 60 65 gta ggt ttg ctg gca gct ggt cct tta tct gac cgc tat ggc cga cgt 355 Val Gly Leu Leu Ala Ala Gly Pro Leu Ser Asp Arg Tyr Gly Arg Arg  70 75 80 85 gcc gtc atg ttg cct gcg cca ttg atc gcg atc ttg ggt tcc gcg ttg 403 Ala Val Met Leu Pro Ala Pro Leu Ile Ala Ile Leu Gly Ser Ala Leu                  90 95 100 att gcc tcg ggt gaa gaa acc gcc atc ctg att gcc att ggt cga gtg 451 Ile Ala Ser Gly Glu Glu Thr Ala Ile Leu Ile Ala Ile Gly Arg Val             105 110 115 ctg tcg gga att tcg gtg ggc atg gtg atg aca gcg gga ggt tcc tgg 499 Leu Ser Gly Ile Ser Val Gly Met Val Met Thr Ala Gly Gly Ser Trp         120 125 130 att aag gag ctt tca tcg tcg cgg ttt gag cca ggg gtg aaa acc agt 547 Ile Lys Glu Leu Ser Ser Ser Arg Phe Glu Pro Gly Val Lys Thr Ser     135 140 145 gct ggt gca aaa cgc gca tcg atg tct ttg acc ggt ggt ttt gcg ctc 595 Ala Gly Ala Lys Arg Ala Ser Met Ser Leu Thr Gly Gly Phe Ala Leu 150 155 160 165 ggc cca gcg ctt gct ggt gtg atg gca cag tgg ctg cca cta cct gga 643 Gly Pro Ala Leu Ala Gly Val Met Ala Gln Trp Leu Pro Leu Pro Gly                 170 175 180 cag ttg gca tat gtt ttg cac att att ctc act ctg att ttg ttc ccg 691 Gln Leu Ala Tyr Val Leu His Ile Ile Leu Thr Leu Ile Leu Phe Pro             185 190 195 ttg ctt att aca gcg ccg gaa act cgt caa tca gcg cac ctg aaa act 739 Leu Leu Ile Thr Ala Pro Glu Thr Arg Gln Ser Ala His Leu Lys Thr         200 205 210 aag gga tca ttc tgg tca gat gtg ctt gtg cca tct gca cta gac aag 787 Lys Gly Ser Phe Trp Ser Asp Val Leu Val Pro Ser Ala Leu Asp Lys     215 220 225 cga ttc ttg ttt gtg gtt gct cca att gga ccg tgg gtt ttc ggt gcg 835 Arg Phe Leu Phe Val Val Ala Pro Ile Gly Pro Trp Val Phe Gly Ala 230 235 240 245 gcc ttc act gcc tac gca gtt ttg ccg tcg cag ctg cgt gac atg gtt 883 Ala Phe Thr Ala Tyr Ala Val Leu Pro Ser Gln Leu Arg Asp Met Val                 250 255 260 tct gca ccc gtt gcg tat tct gcg ctg atc gct ttg gtt acc tta ggt 931 Ser Ala Pro Val Ala Tyr Ser Ala Leu Ile Ala Leu Val Thr Leu Gly             265 270 275 tct gga ttt ggt atc caa caa ttc ggt cct caa atc atg ggc acc tct 979 Ser Gly Phe Gly Ile Gln Gln Phe Gly Pro Gln Ile Met Gly Thr Ser         280 285 290 aaa act cgc ggg ccg att ttg gcc atg ttc gtc aca gtc atc ggc atg 1027 Lys Thr Arg Gly Pro Ile Leu Ala Met Phe Val Thr Val Ile Gly Met     295 300 305 atc ggc gcg gtg atc gtg gtg atg aac cct cat cca tgg tgg gcg cta 1075 Ile Gly Ala Val Ile Val Val Met Asn Pro His Pro Trp Trp Ala Leu 310 315 320 325 gtt ggc tgc atg gcc ctc ggc ctg tct tat ggc ctg tgt atg ttc atg 1123 Val Gly Cys Met Ala Leu Gly Leu Ser Tyr Gly Leu Cys Met Phe Met                 330 335 340 ggg ttg gcg gaa act caa aac att gct cca cct att gat atg gca ggc 1171 Gly Leu Ala Glu Thr Gln Asn Ile Ala Pro Pro Ile Asp Met Ala Gly             345 350 355 ctg acg ggt att ttc tac tgc ctg acg tac gta ggt atg gtc ttt cca 1219 Leu Thr Gly Ile Phe Tyr Cys Leu Thr Tyr Val Gly Met Val Phe Pro         360 365 370 gcc ttg atg acc tgg ttg aat caa tgg ctc agt tac ccg ttc atg ctg 1267 Ala Leu Met Thr Trp Leu Asn Gln Trp Leu Ser Tyr Pro Phe Met Leu     375 380 385 ggc ttt ggt gcg gtg atg gca act att tgt ctg atc att gtg agt ttt 1315 Gly Phe Gly Ala Val Met Ala Thr Ile Cys Leu Ile Ile Val Ser Phe 390 395 400 405 agt gca cgc cga ttc tgagaaacaa ctaaagtgag cca 1353 Ser Ala Arg Arg Phe                 410 <210> 154 <211> 410 <212> PRT <213> Corynebacterium glutamicum <400> 154 Met Gly Val Ser Ala Leu Asn Met Ser Asp Met Val Ala Asn Lys Arg   1 5 10 15 Ala Gln Arg Lys Val Trp Leu Ala Val Ala Leu Ser Val Phe Thr Val              20 25 30 Ala Trp Gly Gly Asn Glu Phe Thr Pro Leu Leu Val Phe Tyr Arg Gly          35 40 45 Glu Gly Phe Phe Ser Asn Leu Phe Ile Asp Leu Leu Leu Val Phe Tyr      50 55 60 Ala Ile Gly Val Ala Val Gly Leu Leu Ala Ala Gly Pro Leu Ser Asp  65 70 75 80 Arg Tyr Gly Arg Arg Ala Val Met Leu Pro Ala Pro Leu Ile Ala Ile                  85 90 95 Leu Gly Ser Ala Leu Ile Ala Ser Gly Glu Glu Thr Ala Ile Leu Ile             100 105 110 Ala Ile Gly Arg Val Leu Ser Gly Ile Ser Val Gly Met Val Met Thr         115 120 125 Ala Gly Gly Ser Trp Ile Lys Glu Leu Ser Ser Ser Arg Phe Glu Pro     130 135 140 Gly Val Lys Thr Ser Ala Gly Ala Lys Arg Ala Ser Met Ser Leu Thr 145 150 155 160 Gly Gly Phe Ala Leu Gly Pro Ala Leu Ala Gly Val Met Ala Gln Trp                 165 170 175 Leu Pro Leu Pro Gly Gln Leu Ala Tyr Val Leu His Ile Ile Leu Thr             180 185 190 Leu Ile Leu Phe Pro Leu Leu Ile Thr Ala Pro Glu Thr Arg Gln Ser         195 200 205 Ala His Leu Lys Thr Lys Gly Ser Phe Trp Ser Asp Val Leu Val Pro     210 215 220 Ser Ala Leu Asp Lys Arg Phe Leu Phe Val Val Ala Pro Ile Gly Pro 225 230 235 240 Trp Val Phe Gly Ala Ala Phe Thr Ala Tyr Ala Val Leu Pro Ser Gln                 245 250 255 Leu Arg Asp Met Val Ser Ala Pro Val Ala Tyr Ser Ala Leu Ile Ala             260 265 270 Leu Val Thr Leu Gly Ser Gly Phe Gly Ile Gln Gln Phe Gly Pro Gln         275 280 285 Ile Met Gly Thr Ser Lys Thr Arg Gly Pro Ile Leu Ala Met Phe Val     290 295 300 Thr Val Ile Gly Met Ile Gly Ala Val Ile Val Val Met Asn Pro His 305 310 315 320 Pro Trp Trp Ala Leu Val Gly Cys Met Ala Leu Gly Leu Ser Tyr Gly                 325 330 335 Leu Cys Met Phe Met Gly Leu Ala Glu Thr Gln Asn Ile Ala Pro Pro             340 345 350 Ile Asp Met Ala Gly Leu Thr Gly Ile Phe Tyr Cys Leu Thr Tyr Val         355 360 365 Gly Met Val Phe Pro Ala Leu Met Thr Trp Leu Asn Gln Trp Leu Ser     370 375 380 Tyr Pro Phe Met Leu Gly Phe Gly Ala Val Met Ala Thr Ile Cys Leu 385 390 395 400 Ile Ile Val Ser Phe Ser Ala Arg Arg Phe                 405 410 <210> 155 <211> 703 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (703) <223> FRXA00803 <400> 155 tcatccttcc ttagctcgcg tgagcttccc aagcgtaagc acccccgtgt gagggcataa 60 cggccgttct gttaaagatt ggtctggcca tttcctccat atg ggg gtg tcc gcg 115                                             Met Gly Val Ser Ala                                               1 5 ctt aac atg tct gac atg gtg gcg aac aaa cgg gca cag cgt aaa gtc 163 Leu Asn Met Ser Asp Met Val Ala Asn Lys Arg Ala Gln Arg Lys Val                  10 15 20 tgg cta gcg gta gct tta tcg gtc ttt acg gtc gcg tgg ggt ggc aat 211 Trp Leu Ala Val Ala Leu Ser Val Phe Thr Val Ala Trp Gly Gly Asn              25 30 35 gaa ttc act ccc ttg ctg gtg ttt tac cga ggt gaa ggg ttc ttt agc 259 Glu Phe Thr Pro Leu Leu Val Phe Tyr Arg Gly Glu Gly Phe Phe Ser          40 45 50 aac ctg ttc atc gac ctt ttg ctg gtg ttt tat gcc atc gga gta gcg 307 Asn Leu Phe Ile Asp Leu Leu Leu Val Phe Tyr Ala Ile Gly Val Ala      55 60 65 gta ggt ttg ctg gca gct ggt cct tta tct gac cgc tat ggc cga cgt 355 Val Gly Leu Leu Ala Ala Gly Pro Leu Ser Asp Arg Tyr Gly Arg Arg  70 75 80 85 gcc gtc atg ttg cct gcg cca ttg atc gcg atc ttg ggt tcc gcg ttg 403 Ala Val Met Leu Pro Ala Pro Leu Ile Ala Ile Leu Gly Ser Ala Leu                  90 95 100 att gcc tcg ggt gaa gaa acc gcc atc ctg att gcc att ggt cga gtg 451 Ile Ala Ser Gly Glu Glu Thr Ala Ile Leu Ile Ala Ile Gly Arg Val             105 110 115 ctg tcg gga att tcg gtg ggc atg gtg atg aca gcg gga ggt tcc tgg 499 Leu Ser Gly Ile Ser Val Gly Met Val Met Thr Ala Gly Gly Ser Trp         120 125 130 att aag gag ctt tca tcg tcg cgg ttt gag cca ggg gtg aaa acc agt 547 Ile Lys Glu Leu Ser Ser Ser Arg Phe Glu Pro Gly Val Lys Thr Ser     135 140 145 gct ggt gca aaa cgc gca tcg atg tct ttg acc ggt ggt ttt gcg ctc 595 Ala Gly Ala Lys Arg Ala Ser Met Ser Leu Thr Gly Gly Phe Ala Leu 150 155 160 165 ggc cca gcg ctt gct ggt gtg atg gca cag tgg ctg cca caa cct gga 643 Gly Pro Ala Leu Ala Gly Val Met Ala Gln Trp Leu Pro Gln Pro Gly                 170 175 180 cag ttg gca tat gtt ttg cac att att ctc act ctg att ttg ttc ccg 691 Gln Leu Ala Tyr Val Leu His Ile Ile Leu Thr Leu Ile Leu Phe Pro             185 190 195 ttg ctt att aca 703 Leu Leu Ile Thr         200 <210> 156 <211> 201 <212> PRT <213> Corynebacterium glutamicum <400> 156 Met Gly Val Ser Ala Leu Asn Met Ser Asp Met Val Ala Asn Lys Arg   1 5 10 15 Ala Gln Arg Lys Val Trp Leu Ala Val Ala Leu Ser Val Phe Thr Val              20 25 30 Ala Trp Gly Gly Asn Glu Phe Thr Pro Leu Leu Val Phe Tyr Arg Gly          35 40 45 Glu Gly Phe Phe Ser Asn Leu Phe Ile Asp Leu Leu Leu Val Phe Tyr      50 55 60 Ala Ile Gly Val Ala Val Gly Leu Leu Ala Ala Gly Pro Leu Ser Asp  65 70 75 80 Arg Tyr Gly Arg Arg Ala Val Met Leu Pro Ala Pro Leu Ile Ala Ile                  85 90 95 Leu Gly Ser Ala Leu Ile Ala Ser Gly Glu Glu Thr Ala Ile Leu Ile             100 105 110 Ala Ile Gly Arg Val Leu Ser Gly Ile Ser Val Gly Met Val Met Thr         115 120 125 Ala Gly Gly Ser Trp Ile Lys Glu Leu Ser Ser Ser Arg Phe Glu Pro     130 135 140 Gly Val Lys Thr Ser Ala Gly Ala Lys Arg Ala Ser Met Ser Leu Thr 145 150 155 160 Gly Gly Phe Ala Leu Gly Pro Ala Leu Ala Gly Val Met Ala Gln Trp                 165 170 175 Leu Pro Gln Pro Gly Gln Leu Ala Tyr Val Leu His Ile Ile Leu Thr             180 185 190 Leu Ile Leu Phe Pro Leu Leu Ile Thr         195 200 <210> 157 <211> 1014 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) .. (991) <223> RXA01407 <400> 157 atccggggaa cggatcccaa agatctcctt gatgccatcg cgtttttaac ctggccagct 60 ctggttgccc cagtgatcgc cccacttctg ggaggtcttc ttg caa gat acc att 115                                             Leu Gln Asp Thr Ile                                               1 5 ggt tgc cga tgg atc ttc ctc ctc aac gtg ccc tta gga atc atc gcg 163 Gly Cys Arg Trp Ile Phe Leu Leu Asn Val Pro Leu Gly Ile Ile Ala                  10 15 20 atc atg gct gga cta ttc atc cag ccc aag aac acg gcc gtg aat gtg 211 Ile Met Ala Gly Leu Phe Ile Gln Pro Lys Asn Thr Ala Val Asn Val              25 30 35 aag cga ttt gat cgg cca ggt ttc ctc ggc gca atg ctg gtg atg gtg 259 Lys Arg Phe Asp Arg Pro Gly Phe Leu Gly Ala Met Leu Val Met Val          40 45 50 gcg caa gcc gtg att gcg gag tta att tgc agc aga agt ccg gcc gca 307 Ala Gln Ala Val Ile Ala Glu Leu Ile Cys Ser Arg Ser Pro Ala Ala      55 60 65 ctt act atc tgt gca tgc ctc gtc tta agt gct gcg gtg gta tgc ggt 355 Leu Thr Ile Cys Ala Cys Leu Val Leu Ser Ala Ala Val Val Cys Gly  70 75 80 85 ttt gta gtg cgc tgg ctg cga gtt cca ggc cga ctt ttt gat ctc agc 403 Phe Val Val Arg Trp Leu Arg Val Pro Gly Arg Leu Phe Asp Leu Ser                  90 95 100 atc atg cgc atc cca ggt ttc cga gtg ggt aat tcc tcc gga agt atc 451 Ile Met Arg Ile Pro Gly Phe Arg Val Gly Asn Ser Ser Gly Ser Ile             105 110 115 tac cgc ttg gta atc acc gca gca cca ttc atg ttc act ttg ctc ttc 499 Tyr Arg Leu Val Ile Thr Ala Ala Pro Phe Met Phe Thr Leu Leu Phe         120 125 130 caa gtg gcg ttt ggg tgg tct gca aca tta gcg ggt gcc atg gtg gtc 547 Gln Val Ala Phe Gly Trp Ser Ala Thr Leu Ala Gly Ala Met Val Val     135 140 145 gca cta ttc gca ggc aac gtg gca atc aaa cct ttc acc acg ccg atc 595 Ala Leu Phe Ala Gly Asn Val Ala Ile Lys Pro Phe Thr Thr Pro Ile 150 155 160 165 att aaa cgc tgg aat ttc aaa cca gta ctg gtc ttt tct aac gct gct 643 Ile Lys Arg Trp Asn Phe Lys Pro Val Leu Val Phe Ser Asn Ala Ala                 170 175 180 ggc gcc ttg gta ttg gca act ttt ttg ttc gtt cgt gca gat acc cca 691 Gly Ala Leu Val Leu Ala Thr Phe Leu Phe Val Arg Ala Asp Thr Pro             185 190 195 ctg gtt ctc atc gtg ctg ctg ctc ttt gtt tcg ggc gca tta agg tcc 739 Leu Val Leu Ile Val Leu Leu Leu Phe Val Ser Gly Ala Leu Arg Ser         200 205 210 ctc ggt ttc agc gcc tac aac acc ttg cag ttt gtc gat atc tca cca 787 Leu Gly Phe Ser Ala Tyr Asn Thr Leu Gln Phe Val Asp Ile Ser Pro     215 220 225 gaa caa acc agc aac gcc aac gtg tta tca gca acc ctg cac caa cta 835 Glu Gln Thr Ser Asn Ala Asn Val Leu Ser Ala Thr Leu His Gln Leu 230 235 240 245 ggc atg tct ttg ggt att gcg gta gca gtc atc gcc atg tcc ctt gca 883 Gly Met Ser Leu Gly Ile Ala Val Ala Val Ile Ala Met Ser Leu Ala                 250 255 260 ccc acc gcc aac tgg gca ttc cca ctg gca gca gcg ttg ttc ctc att 931 Pro Thr Ala Asn Trp Ala Phe Pro Leu Ala Ala Ala Leu Phe Leu Ile             265 270 275 cct cta atc ggc gca cta tct ttg cct cgc gac ggc ggt gcc cga gcc 979 Pro Leu Ile Gly Ala Leu Ser Leu Pro Arg Asp Gly Gly Ala Arg Ala         280 285 290 ttt tcc tcc tct tagaaaccca cttctgaaag gta 1014 Phe Ser Ser Ser     295 <210> 158 <211> 297 <212> PRT <213> Corynebacterium glutamicum <400> 158 Leu Gln Asp Thr Ile Gly Cys Arg Trp Ile Phe Leu Leu Asn Val Pro   1 5 10 15 Leu Gly Ile Ile Ala Ile Met Ala Gly Leu Phe Ile Gln Pro Lys Asn              20 25 30 Thr Ala Val Asn Val Lys Arg Phe Asp Arg Pro Gly Phe Leu Gly Ala          35 40 45 Met Leu Val Met Val Ala Gln Ala Val Ile Ala Glu Leu Ile Cys Ser      50 55 60 Arg Ser Pro Ala Ala Leu Thr Ile Cys Ala Cys Leu Val Leu Ser Ala  65 70 75 80 Ala Val Val Cys Gly Phe Val Val Arg Trp Leu Arg Val Pro Gly Arg                  85 90 95 Leu Phe Asp Leu Ser Ile Met Arg Ile Pro Gly Phe Arg Val Gly Asn             100 105 110 Ser Ser Gly Ser Ile Tyr Arg Leu Val Ile Thr Ala Ala Pro Phe Met         115 120 125 Phe Thr Leu Leu Phe Gln Val Ala Phe Gly Trp Ser Ala Thr Leu Ala     130 135 140 Gly Ala Met Val Val Ala Leu Phe Ala Gly Asn Val Ala Ile Lys Pro 145 150 155 160 Phe Thr Thr Pro Ile Ile Lys Arg Trp Asn Phe Lys Pro Val Leu Val                 165 170 175 Phe Ser Asn Ala Ala Gly Ala Leu Val Leu Ala Thr Phe Leu Phe Val             180 185 190 Arg Ala Asp Thr Pro Leu Val Leu Ile Val Leu Leu Leu Phe Val Ser         195 200 205 Gly Ala Leu Arg Ser Leu Gly Phe Ser Ala Tyr Asn Thr Leu Gln Phe     210 215 220 Val Asp Ile Ser Pro Glu Gln Thr Ser Asn Ala Asn Val Leu Ser Ala 225 230 235 240 Thr Leu His Gln Leu Gly Met Ser Leu Gly Ile Ala Val Ala Val Ile                 245 250 255 Ala Met Ser Leu Ala Pro Thr Ala Asn Trp Ala Phe Pro Leu Ala Ala             260 265 270 Ala Leu Phe Leu Ile Pro Leu Ile Gly Ala Leu Ser Leu Pro Arg Asp         275 280 285 Gly Gly Ala Arg Ala Phe Ser Ser Ser     290 295 <210> 159 <211> 324 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101) .. (301) <223> RXA01408 <400> 159 cgcatagagt tatctcgaaa ctaccaagac ggtttctttt ctttagatct aaggaggaga 60 gaccgtcttt caccctttca tctgattgga catcgacgcc atg cgc aat gat cgg 115                                             Met Arg Asn Asp Arg                                               1 5 tcc ttt agc gtt ccc att gcg cta ctt gcc gcg gga gca ctg ttt cta 163 Ser Phe Ser Val Pro Ile Ala Leu Leu Ala Ala Gly Ala Leu Phe Leu                  10 15 20 gaa atc ctc gac ggc acc atc ctg aca acc gca gtg cca gct att gct 211 Glu Ile Leu Asp Gly Thr Ile Leu Thr Thr Ala Val Pro Ala Ile Ala              25 30 35 cgt gac ttc ggt att gac gcc gtg gat gtc agc att gca ctg gtt gct 259 Arg Asp Phe Gly Ile Asp Ala Val Asp Val Ser Ile Ala Leu Val Ala          40 45 50 tac ttg gca gcc gca gca gct ggc att ccg ctg cag ggt ggc 301 Tyr Leu Ala Ala Ala Ala Ala Gly Ile Pro Leu Gln Gly Gly      55 60 65 tagcggatcg atttggtgtg cgc 324 <210> 160 <211> 67 <212> PRT <213> Corynebacterium glutamicum <400> 160 Met Arg Asn Asp Arg Ser Phe Ser Val Pro Ile Ala Leu Leu Ala Ala   1 5 10 15 Gly Ala Leu Phe Leu Glu Ile Leu Asp Gly Thr Ile Leu Thr Thr Ala              20 25 30 Val Pro Ala Ile Ala Arg Asp Phe Gly Ile Asp Ala Val Asp Val Ser          35 40 45 Ile Ala Leu Val Ala Tyr Leu Ala Ala Ala Ala Ala Gly Ile Pro Leu      50 55 60 Gln Gly Gly  65 <210> 161 <211> 1275 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1252) <223> RXN01922 <400> 161 acactccttt ggtcacctgg tttggttgag ggaaacagac cgcccaagaa cccaagaaat 60 cccaagaaaa catgctgctt atgaattaaa gtgagcaccc atg aga tca gga aac 115                                             Met Arg Ser Gly Asn                                               1 5 gcc aat cgc gtc ttc ata ggt gtt acc atc ctg ctg ttt act gca gga 163 Ala Asn Arg Val Phe Ile Gly Val Thr Ile Leu Leu Phe Thr Ala Gly                  10 15 20 tgg gca gcc aat cat ttc gcg tca gtg ttg gtg ttg atc cgt gaa caa 211 Trp Ala Ala Asn His Phe Ala Ser Val Leu Val Leu Ile Arg Glu Gln              25 30 35 tta gac gta tca agc gtg ctg gtc aac ggc gct ttt ggt att tat gca 259 Leu Asp Val Ser Ser Val Leu Val Asn Gly Ala Phe Gly Ile Tyr Ala          40 45 50 ctg gga ctt ctt cca agt ttg ctc gca ggc ggt gtg ctt gcc gac cgt 307 Leu Gly Leu Leu Pro Ser Leu Leu Ala Gly Gly Val Leu Ala Asp Arg      55 60 65 ttt ggt gcc cgc atg gtg gta ctc acc gga ggt gta ctt tct gcg ctt 355 Phe Gly Ala Arg Met Val Val Leu Thr Gly Gly Val Leu Ser Ala Leu  70 75 80 85 gga aac ctt tct ctt tta gcg ttt cat gat ggt cct tcc ctc ctg gta 403 Gly Asn Leu Ser Leu Leu Ala Phe His Asp Gly Pro Ser Leu Leu Val                  90 95 100 gga cga ttc atc gtt ggt ctg ggc gtt gga tta gtc gtc agc gcg ggc 451 Gly Arg Phe Ile Val Gly Leu Gly Val Gly Leu Val Val Ser Ala Gly             105 110 115 acc gca tgg gcg ggc aga ttg cgc gga gca agc ggc gtg aca ttg gcc 499 Thr Ala Trp Ala Gly Arg Leu Arg Gly Ala Ser Gly Val Thr Leu Ala         120 125 130 ggc att att ctg acc gcc ggt ttc atg atg ggg ccg att gtg aca agt 547 Gly Ile Ile Leu Thr Ala Gly Phe Met Met Gly Pro Ile Val Thr Ser     135 140 145 ggg ttg ggg atg gcg tcg aca agc att att acg ccc ttt gcc ata agc 595 Gly Leu Gly Met Ala Ser Thr Ser Ile Ile Thr Pro Phe Ala Ile Ser 150 155 160 165 gtt gcc ctc tcg ctg atc gcg gtg gtt gtg gga ttt gcg ctt ggc gat 643 Val Ala Leu Ser Leu Ile Ala Val Val Val Gly Phe Ala Leu Gly Asp                 170 175 180 gcc cgc agc acc ccg agc gca ctt ggc gca tcc agc gga atc aaa cac 691 Ala Arg Ser Thr Pro Ser Ala Leu Gly Ala Ser Ser Gly Ile Lys His             185 190 195 gaa cga agc atg aaa aag gcc ctc gcg gtg tcc ttg ccg atg gca att 739 Glu Arg Ser Met Lys Lys Ala Leu Ala Val Ser Leu Pro Met Ala Ile         200 205 210 tgg gtg ttc agc tgc atc acc acc tcc ctg atc gtg atg tcc gcg cgc 787 Trp Val Phe Ser Cys Ile Thr Thr Ser Leu Ile Val Met Ser Ala Arg     215 220 225 atc gac tcc acc ttc ggc aac gcc att ctt ctc ccc gga atc ggc gcg 835 Ile Asp Ser Thr Phe Gly Asn Ala Ile Leu Leu Pro Gly Ile Gly Ala 230 235 240 245 gcg atc gcc ttc agc gca ggc ctg atc gca caa ttt tta ggt agg aaa 883 Ala Ile Ala Phe Ser Ala Gly Leu Ile Ala Gln Phe Leu Gly Arg Lys                 250 255 260 ttc gcg tgg ggt cgt ggc tcc gga atc gtg ggc gcg ctg tgt gcc ctc 931 Phe Ala Trp Gly Arg Gly Ser Gly Ile Val Gly Ala Leu Cys Ala Leu             265 270 275 gcg ggt ttt gcg ctg gca gct ttt ggt ggc gac tcc att cca gtg tgg 979 Ala Gly Phe Ala Leu Ala Ala Phe Gly Gly Asp Ser Ile Pro Val Trp         280 285 290 ctt ttc gtt atc gcc tcg atc ctg ttc ggc acc gca tat ggc ctc tgc 1027 Leu Phe Val Ile Ala Ser Ile Leu Phe Gly Thr Ala Tyr Gly Leu Cys     295 300 305 ctg cgc gaa ggc ctc ctc agc atc gaa act tac acg cca ctc aac cga 1075 Leu Arg Glu Gly Leu Leu Ser Ile Glu Thr Tyr Thr Pro Leu Asn Arg 310 315 320 325 cgt ggc acc ggc atc ggc atc tat tat gtg ttc acg tat ttg gga ttc 1123 Arg Gly Thr Gly Ile Gly Ile Tyr Tyr Val Phe Thr Tyr Leu Gly Phe                 330 335 340 ggg ctg cca gtg ctt ctc gac gcc ctc ctc ccg cac ctt ggc gcc tcc 1171 Gly Leu Pro Val Leu Leu Asp Ala Leu Leu Pro His Leu Gly Ala Ser             345 350 355 att ccg ctg tac gcg ctg gcg gcg ctc gcc ctt ggc tcc gca gta atc 1219 Ile Pro Leu Tyr Ala Leu Ala Ala Leu Ala Leu Gly Ser Ala Val Ile         360 365 370 cgc ggc gta caa atc aag cgc ggg tat gtg gtt tagatttcta cctacgacct 1272 Arg Gly Val Gln Ile Lys Arg Gly Tyr Val Val     375 380 gaa 1275 <210> 162 <211> 384 <212> PRT <213> Corynebacterium glutamicum <400> 162 Met Arg Ser Gly Asn Ala Asn Arg Val Phe Ile Gly Val Thr Ile Leu   1 5 10 15 Leu Phe Thr Ala Gly Trp Ala Ala Asn His Phe Ala Ser Val Leu Val              20 25 30 Leu Ile Arg Glu Gln Leu Asp Val Ser Ser Val Leu Val Asn Gly Ala          35 40 45 Phe Gly Ile Tyr Ala Leu Gly Leu Leu Pro Ser Leu Leu Ala Gly Gly      50 55 60 Val Leu Ala Asp Arg Phe Gly Ala Arg Met Val Val Leu Thr Gly Gly  65 70 75 80 Val Leu Ser Ala Leu Gly Asn Leu Ser Leu Leu Ala Phe His Asp Gly                  85 90 95 Pro Ser Leu Leu Val Gly Arg Phe Ile Val Gly Leu Gly Val Gly Leu             100 105 110 Val Val Ser Ala Gly Thr Ala Trp Ala Gly Arg Leu Arg Gly Ala Ser         115 120 125 Gly Val Thr Leu Ala Gly Ile Ile Leu Thr Ala Gly Phe Met Met Gly     130 135 140 Pro Ile Val Thr Ser Gly Leu Gly Met Ala Ser Thr Ser Ile Ile Thr 145 150 155 160 Pro Phe Ala Ile Ser Val Ala Leu Ser Leu Ile Ala Val Val Val Gly                 165 170 175 Phe Ala Leu Gly Asp Ala Arg Ser Thr Pro Ser Ala Leu Gly Ala Ser             180 185 190 Ser Gly Ile Lys His Glu Arg Ser Met Lys Lys Ala Leu Ala Val Ser         195 200 205 Leu Pro Met Ala Ile Trp Val Phe Ser Cys Ile Thr Thr Ser Leu Ile     210 215 220 Val Met Ser Ala Arg Ile Asp Ser Thr Phe Gly Asn Ala Ile Leu Leu 225 230 235 240 Pro Gly Ile Gly Ala Ala Ile Ala Phe Ser Ala Gly Leu Ile Ala Gln                 245 250 255 Phe Leu Gly Arg Lys Phe Ala Trp Gly Arg Gly Ser Gly Ile Val Gly             260 265 270 Ala Leu Cys Ala Leu Ala Gly Phe Ala Leu Ala Ala Phe Gly Gly Asp         275 280 285 Ser Ile Pro Val Trp Leu Phe Val Ile Ala Ser Ile Leu Phe Gly Thr     290 295 300 Ala Tyr Gly Leu Cys Leu Arg Glu Gly Leu Leu Ser Ile Glu Thr Tyr 305 310 315 320 Thr Pro Leu Asn Arg Arg Gly Thr Gly Ile Gly Ile Tyr Tyr Val Phe                 325 330 335 Thr Tyr Leu Gly Phe Gly Leu Pro Val Leu Leu Asp Ala Leu Leu Pro             340 345 350 His Leu Gly Ala Ser Ile Pro Leu Tyr Ala Leu Ala Ala Leu Ala Leu         355 360 365 Gly Ser Ala Val Ile Arg Gly Val Gln Ile Lys Arg Gly Tyr Val Val     370 375 380 <210> 163 <211> 1130 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (1) .. (1107) <223> FRXA01922 <400> 163 ctg ctg ttt act gca gga tgg gca gcc aat cat ttc gcg tca gtg ttg 48 Leu Leu Phe Thr Ala Gly Trp Ala Ala Asn His Phe Ala Ser Val Leu   1 5 10 15 gtg ttg atc cgt gaa caa tta gac gta tca agc gtg ctg gtc aac ggc 96 Val Leu Ile Arg Glu Gln Leu Asp Val Ser Ser Val Leu Val Asn Gly              20 25 30 gct ttt ggt att tat gca ctg gga ctt ctt cca agt ttg ctc gca ggc 144 Ala Phe Gly Ile Tyr Ala Leu Gly Leu Leu Pro Ser Leu Leu Ala Gly          35 40 45 ggt gtg ctt gcc gac cgt ttt ggt gcc cgc atg gtg gta ctc acc gga 192 Gly Val Leu Ala Asp Arg Phe Gly Ala Arg Met Val Val Leu Thr Gly      50 55 60 ggt gta ctt tct gcg ctt gga aac ctt tct ctt tta gcg ttt cat gat 240 Gly Val Leu Ser Ala Leu Gly Asn Leu Ser Leu Leu Ala Phe His Asp  65 70 75 80 ggt cct tcc ctc ctg gta gga cga ttc atc gtt ggt ctg ggc gtt gga 288 Gly Pro Ser Leu Leu Val Gly Arg Phe Ile Val Gly Leu Gly Val Gly                  85 90 95 tta gtc gtc agc gcg ggc acc gca tgg gcg ggc aga ttg cgc gga gca 336 Leu Val Val Ser Ala Gly Thr Ala Trp Ala Gly Arg Leu Arg Gly Ala             100 105 110 agc ggc gtg aca ttg gcc ggc att att ctg acc gcc ggt ttc atg atg 384 Ser Gly Val Thr Leu Ala Gly Ile Ile Leu Thr Ala Gly Phe Met Met         115 120 125 ggg ccg att gtg aca agt ggg ttg ggg atg gcg tcg aca agc att att 432 Gly Pro Ile Val Thr Ser Gly Leu Gly Met Ala Ser Thr Ser Ile Ile     130 135 140 acg ccc ttt gcc ata agc gtt gcc ctc tcg ctg atc gcg gtg gtt gtg 480 Thr Pro Phe Ala Ile Ser Val Ala Leu Ser Leu Ile Ala Val Val Val 145 150 155 160 gga ttt gcg ctt ggc gat gcc cgc agc acc ccg agc gca ctt ggc gca 528 Gly Phe Ala Leu Gly Asp Ala Arg Ser Thr Pro Ser Ala Leu Gly Ala                 165 170 175 tcc agc gga atc aaa cac gaa cga agc atg aaa aag gcc ctc gcg gtg 576 Ser Ser Gly Ile Lys His Glu Arg Ser Met Lys Lys Ala Leu Ala Val             180 185 190 tcc ttg ccg atg gca att tgg gtg ttc agc tgc atc acc acc tcc ctg 624 Ser Leu Pro Met Ala Ile Trp Val Phe Ser Cys Ile Thr Thr Ser Leu         195 200 205 atc gtg atg tcc gcg cgc atc gac tcc acc ttc ggc aac gcc att ctt 672 Ile Val Met Ser Ala Arg Ile Asp Ser Thr Phe Gly Asn Ala Ile Leu     210 215 220 ctc ccc gga atc ggc gcg gcg atc gcc ttc agc gca ggc ctg atc gca 720 Leu Pro Gly Ile Gly Ala Ala Ile Ala Phe Ser Ala Gly Leu Ile Ala 225 230 235 240 caa ttt tta ggt agg aaa ttc gcg tgg ggt cgt ggc tcc gga atc gtg 768 Gln Phe Leu Gly Arg Lys Phe Ala Trp Gly Arg Gly Ser Gly Ile Val                 245 250 255 ggc gcg ctg tgt gcc ctc gcg ggt ttt gcg ctg gca gct ttt ggt ggc 816 Gly Ala Leu Cys Ala Leu Ala Gly Phe Ala Leu Ala Ala Phe Gly Gly             260 265 270 gac tcc att cca gtg tgg ctt ttc gtt atc gcc tcg atc ctg ttc ggc 864 Asp Ser Ile Pro Val Trp Leu Phe Val Ile Ala Ser Ile Leu Phe Gly         275 280 285 acc gca tat ggc ctc tgc ctg cgc gaa ggc ctc ctc agc atc gaa act 912 Thr Ala Tyr Gly Leu Cys Leu Arg Glu Gly Leu Leu Ser Ile Glu Thr     290 295 300 tac acg cca ctc aac cga cgt ggc acc ggc atc ggc atc tat tat gtg 960 Tyr Thr Pro Leu Asn Arg Arg Gly Thr Gly Ile Gly Ile Tyr Tyr Val 305 310 315 320 ttc acg tat ttg gga ttc ggg ctg cca gtg ctt ctc gac gcc ctc ctc 1008 Phe Thr Tyr Leu Gly Phe Gly Leu Pro Val Leu Leu Asp Ala Leu Leu                 325 330 335 ccg cac ctt ggc gcc tcc att ccg ctg tac gcg ctg gcg gcg ctc gcc 1056 Pro His Leu Gly Ala Ser Ile Pro Leu Tyr Ala Leu Ala Ala Leu Ala             340 345 350 ctt ggc tcc gca gta atc cgc ggc gta caa atc aag cgc ggg tat gtg 1104 Leu Gly Ser Ala Val Ile Arg Gly Val Gln Ile Lys Arg Gly Tyr Val         355 360 365 gtt tagatttcta cctacgacct gaa 1130 Val      <210> 164 <211> 369 <212> PRT <213> Corynebacterium glutamicum <400> 164 Leu Leu Phe Thr Ala Gly Trp Ala Ala Asn His Phe Ala Ser Val Leu   1 5 10 15 Val Leu Ile Arg Glu Gln Leu Asp Val Ser Ser Val Leu Val Asn Gly              20 25 30 Ala Phe Gly Ile Tyr Ala Leu Gly Leu Leu Pro Ser Leu Leu Ala Gly          35 40 45 Gly Val Leu Ala Asp Arg Phe Gly Ala Arg Met Val Val Leu Thr Gly      50 55 60 Gly Val Leu Ser Ala Leu Gly Asn Leu Ser Leu Leu Ala Phe His Asp  65 70 75 80 Gly Pro Ser Leu Leu Val Gly Arg Phe Ile Val Gly Leu Gly Val Gly                  85 90 95 Leu Val Val Ser Ala Gly Thr Ala Trp Ala Gly Arg Leu Arg Gly Ala             100 105 110 Ser Gly Val Thr Leu Ala Gly Ile Ile Leu Thr Ala Gly Phe Met Met         115 120 125 Gly Pro Ile Val Thr Ser Gly Leu Gly Met Ala Ser Thr Ser Ile Ile     130 135 140 Thr Pro Phe Ala Ile Ser Val Ala Leu Ser Leu Ile Ala Val Val Val 145 150 155 160 Gly Phe Ala Leu Gly Asp Ala Arg Ser Thr Pro Ser Ala Leu Gly Ala                 165 170 175 Ser Ser Gly Ile Lys His Glu Arg Ser Met Lys Lys Ala Leu Ala Val             180 185 190 Ser Leu Pro Met Ala Ile Trp Val Phe Ser Cys Ile Thr Thr Ser Leu         195 200 205 Ile Val Met Ser Ala Arg Ile Asp Ser Thr Phe Gly Asn Ala Ile Leu     210 215 220 Leu Pro Gly Ile Gly Ala Ala Ile Ala Phe Ser Ala Gly Leu Ile Ala 225 230 235 240 Gln Phe Leu Gly Arg Lys Phe Ala Trp Gly Arg Gly Ser Gly Ile Val                 245 250 255 Gly Ala Leu Cys Ala Leu Ala Gly Phe Ala Leu Ala Ala Phe Gly Gly             260 265 270 Asp Ser Ile Pro Val Trp Leu Phe Val Ile Ala Ser Ile Leu Phe Gly         275 280 285 Thr Ala Tyr Gly Leu Cys Leu Arg Glu Gly Leu Leu Ser Ile Glu Thr     290 295 300 Tyr Thr Pro Leu Asn Arg Arg Gly Thr Gly Ile Gly Ile Tyr Tyr Val 305 310 315 320 Phe Thr Tyr Leu Gly Phe Gly Leu Pro Val Leu Leu Asp Ala Leu Leu                 325 330 335 Pro His Leu Gly Ala Ser Ile Pro Leu Tyr Ala Leu Ala Ala Leu Ala             340 345 350 Leu Gly Ser Ala Val Ile Arg Gly Val Gln Ile Lys Arg Gly Tyr Val         355 360 365 Val      <210> 165 <211> 362 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (1) .. (339) <223> RXA02060 <400> 165 gaa ttt gcc cgc att ttg aag cca aag gga cag gtc atc gtg ctt acc 48 Glu Phe Ala Arg Ile Leu Lys Pro Lys Gly Gln Val Ile Val Leu Thr   1 5 10 15 gca gat acc ggc cac ttg gct gag ctt cgt gaa cca ctg ggc atc att 96 Ala Asp Thr Gly His Leu Ala Glu Leu Arg Glu Pro Leu Gly Ile Ile              20 25 30 gat gtg gag gcc ggc aaa gtt gat cgc atg atc gaa caa gcg gca ggc 144 Asp Val Glu Ala Gly Lys Val Asp Arg Met Ile Glu Gln Ala Ala Gly          35 40 45 cac ctc aag cca gtt ggc gaa aga gac ttg gtg gaa ttt gaa atg ctg 192 His Leu Lys Pro Val Gly Glu Arg Asp Leu Val Glu Phe Glu Met Leu      50 55 60 ctg gat caa aaa tcc att gca tct cag atc ggt atg agc cct tct gca 240 Leu Asp Gln Lys Ser Ile Ala Ser Gln Ile Gly Met Ser Pro Ser Ala  65 70 75 80 cgc cac att aag cct gag gct ttg gcg gaa cgc atc gcc gct cta cca 288 Arg His Ile Lys Pro Glu Ala Leu Ala Glu Arg Ile Ala Ala Leu Pro                  85 90 95 gaa caa atg aag gtt aca gcc cgg gcc aag atc acc agg ctg gaa cgc 336 Glu Gln Met Lys Val Thr Ala Arg Ala Lys Ile Thr Arg Leu Glu Arg             100 105 110 atc taactcttat ctcactgggc ctt 362 Ile      <210> 166 <211> 113 <212> PRT <213> Corynebacterium glutamicum <400> 166 Glu Phe Ala Arg Ile Leu Lys Pro Lys Gly Gln Val Ile Val Leu Thr   1 5 10 15 Ala Asp Thr Gly His Leu Ala Glu Leu Arg Glu Pro Leu Gly Ile Ile              20 25 30 Asp Val Glu Ala Gly Lys Val Asp Arg Met Ile Glu Gln Ala Ala Gly          35 40 45 His Leu Lys Pro Val Gly Glu Arg Asp Leu Val Glu Phe Glu Met Leu      50 55 60 Leu Asp Gln Lys Ser Ile Ala Ser Gln Ile Gly Met Ser Pro Ser Ala  65 70 75 80 Arg His Ile Lys Pro Glu Ala Leu Ala Glu Arg Ile Ala Ala Leu Pro                  85 90 95 Glu Gln Met Lys Val Thr Ala Arg Ala Lys Ile Thr Arg Leu Glu Arg             100 105 110 Ile      <210> 167 <211> 1395 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (1372) <223> RXN01936 <400> 167 gcgcggtgac accacagccg ttgtcagcgg cgcttggtct gtggaggatc gccgaggtta 60 ctaacaaata ggcccaacaa agaggtctaa gctctacctg gtg agt ttc cga gat 115                                             Val Ser Phe Arg Asp                                               1 5 att ttc gct gac acc aga ccg ctg aaa gaa ccg gcc ttc aaa cgc ctc 163 Ile Phe Ala Asp Thr Arg Pro Leu Lys Glu Pro Ala Phe Lys Arg Leu                  10 15 20 tgg ctt ggc aat gtt gcc acc gtc att ggt gcc caa tta act gtt gtt 211 Trp Leu Gly Asn Val Ala Thr Val Ile Gly Ala Gln Leu Thr Val Val              25 30 35 gcc gtt ccg gtg cag att tac caa atg act ggg tcc tcc ggc tat gtg 259 Ala Val Pro Val Gln Ile Tyr Gln Met Thr Gly Ser Ser Gly Tyr Val          40 45 50 ggc ttg acc ggg ctt ttt ggc ctt att cct ttg gtt att ttt ggc ctt 307 Gly Leu Thr Gly Leu Phe Gly Leu Ile Pro Leu Val Ile Phe Gly Leu      55 60 65 tat ggt gga tcc att gcg gat gct ttt gat aaa cgc atc gtg ctg atc 355 Tyr Gly Gly Ser Ile Ala Asp Ala Phe Asp Lys Arg Ile Val Leu Ile  70 75 80 85 tgc acc acg atc ggc atg tgt gtc acc act gcc ggt ttt tgg gtg ctg 403 Cys Thr Thr Ile Gly Met Cys Val Thr Thr Ala Gly Phe Trp Val Leu                  90 95 100 acc att tta ggc aat gag aat att tgg ctc ctg tta ata aac ttt tct 451 Thr Ile Leu Gly Asn Glu Asn Ile Trp Leu Leu Leu Ile Asn Phe Ser             105 110 115 tta cag cag gca ttt ttc gcg gtg aat caa ccc acc cga acg gcg atc 499 Leu Gln Gln Ala Phe Phe Ala Val Asn Gln Pro Thr Arg Thr Ala Ile         120 125 130 ctt cga agt att ttg ccg att gat caa tta gcg tcg gca aca tca ctg 547 Leu Arg Ser Ile Leu Pro Ile Asp Gln Leu Ala Ser Ala Thr Ser Leu     135 140 145 aat atg ctg ctc atg cag acc ggc gca atc gtt ggc ccg ctg atc gca 595 Asn Met Leu Leu Met Gln Thr Gly Ala Ile Val Gly Pro Leu Ile Ala 150 155 160 165 ggt gcg ttg att ccg ctg atc ggt ttc ggg tgg ctg tat ttc ctt gat 643 Gly Ala Leu Ile Pro Leu Ile Gly Phe Gly Trp Leu Tyr Phe Leu Asp                 170 175 180 gtt gtc tcc atc atc ccc aca ctg tgg gct gta tgg tca ctg cct tcg 691 Val Val Ser Ile Ile Pro Thr Leu Trp Ala Val Trp Ser Leu Pro Ser             185 190 195 atc aag cca tcc ggc aag gtg atg aag gct ggt ttc gcc agt gtg gtg 739 Ile Lys Pro Ser Gly Lys Val Met Lys Ala Gly Phe Ala Ser Val Val         200 205 210 gat ggc ctg aag tat ttg gct ggc caa ccc gtg ttg ttg atg gtg atg 787 Asp Gly Leu Lys Tyr Leu Ala Gly Gln Pro Val Leu Leu Met Val Met     215 220 225 gtg ctg gat ctt atc gcc atg att ttc ggc atg cca cgt gcg ctt tac 835 Val Leu Asp Leu Ile Ala Met Ile Phe Gly Met Pro Arg Ala Leu Tyr 230 235 240 245 ccc gag atc gca gaa gtg aac ttc ggt ggg ggt gac gcc ggt gca acg 883 Pro Glu Ile Ala Glu Val Asn Phe Gly Gly Gly Asp Ala Gly Ala Thr                 250 255 260 atg ctg gcg ttc atg tac tca tcc atg gct gtt ggc gca gtt ctt ggc 931 Met Leu Ala Phe Met Tyr Ser Ser Met Ala Val Gly Ala Val Leu Gly             265 270 275 ggc gtg ctg tct ggt tgg gtg gcc cgg att agc cgc cag ggt gtt gca 979 Gly Val Leu Ser Gly Trp Val Ala Arg Ile Ser Arg Gln Gly Val Ala         280 285 290 gtt tat tgg tgc atc atc gcc tgg ggc gca gcc gtt gct ttg ggt ggt 1027 Val Tyr Trp Cys Ile Ile Ala Trp Gly Ala Ala Val Ala Leu Gly Gly     295 300 305 gtg gca att gtt gtc agc ccc ggc gcg gtg act gcg tgg gcg tgg atg 1075 Val Ala Ile Val Val Ser Pro Gly Ala Val Thr Ala Trp Ala Trp Met 310 315 320 325 ttc atc atc atg atg gtc att ggt ggc atg gct gac atg ttc agc tcg 1123 Phe Ile Ile Met Met Val Ile Gly Gly Met Ala Asp Met Phe Ser Ser                 330 335 340 gca gtt cga aac gct att ttg cag cag tct gct gcg gaa cat gtg cag 1171 Ala Val Arg Asn Ala Ile Leu Gln Gln Ser Ala Ala Glu His Val Gln             345 350 355 ggc cga atc caa ggt gtg tgg atc atc gtc gtg gtg ggt gga cct cgt 1219 Gly Arg Ile Gln Gly Val Trp Ile Ile Val Val Val Gly Gly Pro Arg         360 365 370 tta gct gac gtc ctt cac ggt tgg gcc gct gag ccc ctc ggc gca ggt 1267 Leu Ala Asp Val Leu His Gly Trp Ala Ala Glu Pro Leu Gly Ala Gly     375 380 385 tgg acg gta tta tgg ggc gga gta gcg gtg gtt gta ctc act gca att 1315 Trp Thr Val Leu Trp Gly Gly Val Ala Val Val Val Leu Thr Ala Ile 390 395 400 405 tgt atg gtg gcg gtg cct aaa ttc tgg aaa tac gag aaa cca aaa att 1363 Cys Met Val Ala Val Pro Lys Phe Trp Lys Tyr Glu Lys Pro Lys Ile                 410 415 420 acc ggc atc taaatactta tccatgccca ttt 1395 Thr Gly Ile              <210> 168 <211> 424 <212> PRT <213> Corynebacterium glutamicum <400> 168 Val Ser Phe Arg Asp Ile Phe Ala Asp Thr Arg Pro Leu Lys Glu Pro   1 5 10 15 Ala Phe Lys Arg Leu Trp Leu Gly Asn Val Ala Thr Val Ile Gly Ala              20 25 30 Gln Leu Thr Val Val Ala Val Pro Val Gln Ile Tyr Gln Met Thr Gly          35 40 45 Ser Ser Gly Tyr Val Gly Leu Thr Gly Leu Phe Gly Leu Ile Pro Leu      50 55 60 Val Ile Phe Gly Leu Tyr Gly Gly Ser Ile Ala Asp Ala Phe Asp Lys  65 70 75 80 Arg Ile Val Leu Ile Cys Thr Thr Ile Gly Met Cys Val Thr Thr Ala                  85 90 95 Gly Phe Trp Val Leu Thr Ile Leu Gly Asn Glu Asn Ile Trp Leu Leu             100 105 110 Leu Ile Asn Phe Ser Leu Gln Gln Ala Phe Phe Ala Val Asn Gln Pro         115 120 125 Thr Arg Thr Ala Ile Leu Arg Ser Ile Leu Pro Ile Asp Gln Leu Ala     130 135 140 Ser Ala Thr Ser Leu Asn Met Leu Leu Met Gln Thr Gly Ala Ile Val 145 150 155 160 Gly Pro Leu Ile Ala Gly Ala Leu Ile Pro Leu Ile Gly Phe Gly Trp                 165 170 175 Leu Tyr Phe Leu Asp Val Val Ser Ile Pro Thr Leu Trp Ala Val             180 185 190 Trp Ser Leu Pro Ser Ile Lys Pro Ser Gly Lys Val Met Lys Ala Gly         195 200 205 Phe Ala Ser Val Val Asp Gly Leu Lys Tyr Leu Ala Gly Gln Pro Val     210 215 220 Leu Leu Met Val Met Val Leu Asp Leu Ile Ala Met Ile Phe Gly Met 225 230 235 240 Pro Arg Ala Leu Tyr Pro Glu Ile Ala Glu Val Asn Phe Gly Gly Gly                 245 250 255 Asp Ala Gly Ala Thr Met Leu Ala Phe Met Tyr Ser Ser Met Ala Val             260 265 270 Gly Ala Val Leu Gly Gly Val Leu Ser Gly Trp Val Ala Arg Ile Ser         275 280 285 Arg Gln Gly Val Ala Val Tyr Trp Cys Ile Ile Ala Trp Gly Ala Ala     290 295 300 Val Ala Leu Gly Gly Val Ala Ile Val Val Ser Pro Gly Ala Val Thr 305 310 315 320 Ala Trp Ala Trp Met Phe Ile Ile Met Met Val Ile Gly Gly Met Ala                 325 330 335 Asp Met Phe Ser Ser Ala Val Arg Asn Ala Ile Leu Gln Gln Ser Ala             340 345 350 Ala Glu His Val Gln Gly Arg Ile Gln Gly Val Trp Ile Ile Val Val         355 360 365 Val Gly Gly Pro Arg Leu Ala Asp Val Leu His Gly Trp Ala Ala Glu     370 375 380 Pro Leu Gly Ala Gly Trp Thr Val Leu Trp Gly Gly Val Ala Val Val 385 390 395 400 Val Leu Thr Ala Ile Cys Met Val Ala Val Pro Lys Phe Trp Lys Tyr                 405 410 415 Glu Lys Pro Lys Ile Thr Gly Ile             420 <210> 169 <211> 945 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (922) <223> FRXA01936 <400> 169 tttacagcag gcatttttcg cggtgaatca acccacccga acggcgatcc ttcgaagtat 60 tttgccgatt gatcaataag cgtcggcaac atcactgaat atg ctg ctc atg cag 115                                             Met Leu Leu Met Gln                                               1 5 acc ggc gca atc gtt ggc ccg ctg atc gca ggt gcg ttg att ccg ctg 163 Thr Gly Ala Ile Val Gly Pro Leu Ile Ala Gly Ala Leu Ile Pro Leu                  10 15 20 atc ggt ttc ggg tgg ctg tat ttc ctt gat gtt gtc tcc atc atc ccc 211 Ile Gly Phe Gly Trp Leu Tyr Phe Leu Asp Val Val Ser Ile Ile Pro              25 30 35 aca ctg tgg gct gta tgg tca ctg cct tcg atc aag cca tcc ggc aag 259 Thr Leu Trp Ala Val Trp Ser Leu Pro Ser Ile Lys Pro Ser Gly Lys          40 45 50 gtg atg aag gct ggt ttc gcc agt gtg gtg gat ggc ctg aag tat ttg 307 Val Met Lys Ala Gly Phe Ala Ser Val Val Asp Gly Leu Lys Tyr Leu      55 60 65 gct ggc caa ccc gtg ttg ttg atg gtg atg gtg ctg gat ctt atc gcc 355 Ala Gly Gln Pro Val Leu Leu Met Val Met Val Leu Asp Leu Ile Ala  70 75 80 85 atg att ttc ggc atg cca cgt gcg ctt tac ccc gag atc gca gaa gtg 403 Met Ile Phe Gly Met Pro Arg Ala Leu Tyr Pro Glu Ile Ala Glu Val                  90 95 100 aac ttc ggt ggg ggt gac gcc ggt gca acg atg ctg gcg ttc atg tac 451 Asn Phe Gly Gly Gly Asp Ala Gly Ala Thr Met Leu Ala Phe Met Tyr             105 110 115 tca tcc atg gct gtt ggc gca gtt ctt ggc ggc gtg ctg tct ggt tgg 499 Ser Ser Met Ala Val Gly Ala Val Leu Gly Gly Val Leu Ser Gly Trp         120 125 130 gtg gcc cgg att agc cgc cag ggt gtt gca gtt tat tgg tgc atc atc 547 Val Ala Arg Ile Ser Arg Gln Gly Val Ala Val Tyr Trp Cys Ile Ile     135 140 145 gcc tgg ggc gca gcc gtt gct ttg ggt ggt gtg gca att gtt gtc agc 595 Ala Trp Gly Ala Ala Val Ala Leu Gly Gly Val Ala Ile Val Val Ser 150 155 160 165 ccc ggc gcg gtg act gcg tgg gcg tgg atg ttc atc atc atg atg gtc 643 Pro Gly Ala Val Thr Ala Trp Ala Trp Met Phe Ile Ile Met Met Val                 170 175 180 att ggt ggc atg gct gac atg ttc agc tcg gca gtt cga aac gct att 691 Ile Gly Gly Met Ala Asp Met Phe Ser Ser Ala Val Arg Asn Ala Ile             185 190 195 ttg cag cag tct gct gcg gaa cat gtg cag ggc cga atc caa ggt gtg 739 Leu Gln Gln Ser Ala Ala Glu His Val Gln Gly Arg Ile Gln Gly Val         200 205 210 tgg atc atc gtc gtg gtg ggt gga cct cgt tta gct gac gtc ctt cac 787 Trp Ile Ile Val Val Val Gly Gly Pro Arg Leu Ala Asp Val Leu His     215 220 225 ggt tgg gcc gct gag ccc ctc ggc gca ggt tgg acg gta tta tgg ggc 835 Gly Trp Ala Ala Glu Pro Leu Gly Ala Gly Trp Thr Val Leu Trp Gly 230 235 240 245 gga gta gcg gtg gtt gta ctc act gca att tgt atg gtg gcg gtg cct 883 Gly Val Ala Val Val Val Leu Thr Ala Ile Cys Met Val Ala Val Pro                 250 255 260 aaa ttc tgg aaa tac gag aaa cca aaa att acc ggc atc taaatactta 932 Lys Phe Trp Lys Tyr Glu Lys Pro Lys Ile Thr Gly Ile             265 270 tccatgccca ttt 945 <210> 170 <211> 274 <212> PRT <213> Corynebacterium glutamicum <400> 170 Met Leu Leu Met Gln Thr Gly Ala Ile Val Gly Pro Leu Ile Ala Gly   1 5 10 15 Ala Leu Ile Pro Leu Ile Gly Phe Gly Trp Leu Tyr Phe Leu Asp Val              20 25 30 Val Ser Ile Ile Pro Thr Leu Trp Ala Val Trp Ser Leu Pro Ser Ile          35 40 45 Lys Pro Ser Gly Lys Val Met Lys Ala Gly Phe Ala Ser Val Val Asp      50 55 60 Gly Leu Lys Tyr Leu Ala Gly Gln Pro Val Leu Leu Met Val Met Val  65 70 75 80 Leu Asp Leu Ile Ala Met Ile Phe Gly Met Pro Arg Ala Leu Tyr Pro                  85 90 95 Glu Ile Ala Glu Val Asn Phe Gly Gly Gly Asp Ala Gly Ala Thr Met             100 105 110 Leu Ala Phe Met Tyr Ser Ser Met Ala Val Gly Ala Val Leu Gly Gly         115 120 125 Val Leu Ser Gly Trp Val Ala Arg Ile Ser Arg Gln Gly Val Ala Val     130 135 140 Tyr Trp Cys Ile Ile Ala Trp Gly Ala Ala Val Ala Leu Gly Gly Val 145 150 155 160 Ala Ile Val Val Ser Pro Gly Ala Val Thr Ala Trp Ala Trp Met Phe                 165 170 175 Ile Ile Met Met Val Ile Gly Gly Met Ala Asp Met Phe Ser Ser Ala             180 185 190 Val Arg Asn Ala Ile Leu Gln Gln Ser Ala Ala Glu His Val Gln Gly         195 200 205 Arg Ile Gln Gly Val Trp Ile Ile Val Val Val Gly Gly Pro Arg Leu     210 215 220 Ala Asp Val Leu His Gly Trp Ala Ala Glu Pro Leu Gly Ala Gly Trp 225 230 235 240 Thr Val Leu Trp Gly Gly Val Ala Val Val Val Leu Thr Ala Ile Cys                 245 250 255 Met Val Ala Val Pro Lys Phe Trp Lys Tyr Glu Lys Pro Lys Ile Thr             260 265 270 Gly ile          <210> 171 <211> 549 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (526) <223> FRXA01937 <400> 171 gcgcggtgac accacagccg ttgtcagcgg cgcttggtct gtggaggatc gccgaggtta 60 ctaacaaata ggcccaacaa agaggtctaa gctctacctg gtg agt ttc cga gat 115                                             Val Ser Phe Arg Asp                                               1 5 att ttc gct gac acc aga ccg ctg aaa gaa ccg gcc ttc aaa cgc ctc 163 Ile Phe Ala Asp Thr Arg Pro Leu Lys Glu Pro Ala Phe Lys Arg Leu                  10 15 20 tgg ctt ggc aat gtt gcc acc gtc att ggt gcc caa tta act gtt gtt 211 Trp Leu Gly Asn Val Ala Thr Val Ile Gly Ala Gln Leu Thr Val Val              25 30 35 gcc gtt ccg gtg cag att tac caa atg act ggg tcc tcc ggc tat gtg 259 Ala Val Pro Val Gln Ile Tyr Gln Met Thr Gly Ser Ser Gly Tyr Val          40 45 50 ggc ttg acc ggg ctt ttt ggc ctt att cct ttg gtt att ttt ggc ctt 307 Gly Leu Thr Gly Leu Phe Gly Leu Ile Pro Leu Val Ile Phe Gly Leu      55 60 65 tat ggt gga tcc att gcg gat gct ttt gat aaa cgc atc gtg ctg atc 355 Tyr Gly Gly Ser Ile Ala Asp Ala Phe Asp Lys Arg Ile Val Leu Ile  70 75 80 85 tgc acc acg atc ggc atg tgt gtc acc act gcc ggt ttt tgg gtg ctg 403 Cys Thr Thr Ile Gly Met Cys Val Thr Thr Ala Gly Phe Trp Val Leu                  90 95 100 acc att tta ggc aat gag aat att tgg ctc ctg tta ata aac ttt tct 451 Thr Ile Leu Gly Asn Glu Asn Ile Trp Leu Leu Leu Ile Asn Phe Ser             105 110 115 tta cag cag gca ttt ttc gcg gtg aat caa ccc acc cga acg gcg atc 499 Leu Gln Gln Ala Phe Phe Ala Val Asn Gln Pro Thr Arg Thr Ala Ile         120 125 130 ctt cga agt att ttg ccg att gat caa taagcgtcgg caacatcact 546 Leu Arg Ser Ile Leu Pro Ile Asp Gln     135 140 gaa 549 <210> 172 <211> 142 <212> PRT <213> Corynebacterium glutamicum <400> 172 Val Ser Phe Arg Asp Ile Phe Ala Asp Thr Arg Pro Leu Lys Glu Pro   1 5 10 15 Ala Phe Lys Arg Leu Trp Leu Gly Asn Val Ala Thr Val Ile Gly Ala              20 25 30 Gln Leu Thr Val Val Ala Val Pro Val Gln Ile Tyr Gln Met Thr Gly          35 40 45 Ser Ser Gly Tyr Val Gly Leu Thr Gly Leu Phe Gly Leu Ile Pro Leu      50 55 60 Val Ile Phe Gly Leu Tyr Gly Gly Ser Ile Ala Asp Ala Phe Asp Lys  65 70 75 80 Arg Ile Val Leu Ile Cys Thr Thr Ile Gly Met Cys Val Thr Thr Ala                  85 90 95 Gly Phe Trp Val Leu Thr Ile Leu Gly Asn Glu Asn Ile Trp Leu Leu             100 105 110 Leu Ile Asn Phe Ser Leu Gln Gln Ala Phe Phe Ala Val Asn Gln Pro         115 120 125 Thr Arg Thr Ala Ile Leu Arg Ser Ile Leu Pro Ile Asp Gln     130 135 140 <210> 173 <211> 1242 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1219) <223> RXN01010 <400> 173 gtgccaaagc gtttcctgta aaacgcataa ccccgaatac cccctgtttc cagatccaaa 60 aaaagatctg gcagggggtt taggcataga ttaggaactt atg aag aaa ctg caa 115                                             Met Lys Lys Leu Gln                                               1 5 atg ccg gcc att ttg gtc gga ggc ttt gtg ggg ccg ttt act ggc caa 163 Met Pro Ala Ile Leu Val Gly Gly Phe Val Gly Pro Phe Thr Gly Gln                  10 15 20 gct cta tca gtg gtc ttg ccg gaa ttt gca gac acc ttt gat atc agt 211 Ala Leu Ser Val Val Leu Pro Glu Phe Ala Asp Thr Phe Asp Ile Ser              25 30 35 gtc agc cag gca gcg ctg acc atg acc gca tac ttg ttg ccc ttt gcc 259 Val Ser Gln Ala Ala Leu Thr Met Thr Ala Tyr Leu Leu Pro Phe Ala          40 45 50 acc atg atg ttg ttt tcg ggg cgc atc acc aga aag atc cat ccg cat 307 Thr Met Met Leu Phe Ser Gly Arg Ile Thr Arg Lys Ile His Pro His      55 60 65 aag gtg gtg cag gcg gct tat att gtc aca ctg cca ctt gcg ctg ttg 355 Lys Val Val Gln Ala Ala Tyr Ile Val Thr Leu Pro Leu Ala Leu Leu  70 75 80 85 ctc cta gtt aca cca tcg tgg ggg ctg ttt atg gct gcg tat gcc acg 403 Leu Leu Val Thr Pro Ser Trp Gly Leu Phe Met Ala Ala Tyr Ala Thr                  90 95 100 att ggt atc gct aat gca ttt acc act ccg gtg ctg caa att atg ttg 451 Ile Gly Ile Ala Asn Ala Phe Thr Thr Pro Val Leu Gln Ile Met Leu             105 110 115 cgt gag ctt gtt ccg ccg cgt tct ttg ggt aag gca ttg ggc acc tat 499 Arg Glu Leu Val Pro Pro Arg Ser Leu Gly Lys Ala Leu Gly Thr Tyr         120 125 130 gct gcg atg caa tca ctc ggc atg ttg tcg gcg cca ctg atc gca ggt 547 Ala Ala Met Gln Ser Leu Gly Met Leu Ser Ala Pro Leu Ile Ala Gly     135 140 145 gtg tct tcg gtg gtg tcg tgg agg ttg acc ttc ctg gtc act gca gca 595 Val Ser Ser Val Val Ser Trp Arg Leu Thr Phe Leu Val Thr Ala Ala 150 155 160 165 gcg tca ctg ttt att ttg gtg gcg cga ctc ccc gtt gtt cca cca cca 643 Ala Ser Leu Phe Ile Leu Val Ala Arg Leu Pro Val Val Pro Pro Pro                 170 175 180 tca gca tcg aag caa aac gtt agt ggc aag gtg cag tgg gga ccg acc 691 Ser Ala Ser Lys Gln Asn Val Ser Gly Lys Val Gln Trp Gly Pro Thr             185 190 195 atc atc cac atg gtt tcc ggc ttt gtg gtg ggc atc ggc atc atc ggc 739 Ile Ile His Met Val Ser Gly Phe Val Val Gly Ile Gly Ile Ile Gly         200 205 210 att gga ttc atg aca tcg ctg cac gtt ggc gag caa ttc gga ctt gat 787 Ile Gly Phe Met Thr Ser Leu His Val Gly Glu Gln Phe Gly Leu Asp     215 220 225 gct gca gcg cgt ggt ttg gtg gtc atg tgt ggt ggc ctg gct gcg ttc 835 Ala Ala Ala Arg Gly Leu Val Val Met Cys Gly Gly Leu Ala Ala Phe 230 235 240 245 ttt gcc tcc cgc aag att ggc gat ttg gca gac aaa ttt ggt gtg cgc 883 Phe Ala Ser Arg Lys Ile Gly Asp Leu Ala Asp Lys Phe Gly Val Arg                 250 255 260 gcg gtg ctc att gtc agt gct gtc atc ggt acc atc gca ctc gca ctg 931 Ala Val Leu Ile Val Ser Ala Val Ile Gly Thr Ile Ala Leu Ala Leu             265 270 275 ctg ccg atc gca ccg tgg atc att gtg gtg gcc gta ctg tgg gcc ttc 979 Leu Pro Ile Ala Pro Trp Ile Ile Val Val Ala Val Leu Trp Ala Phe         280 285 290 gca gta gca gca gca caa gga atc caa gca acc gtc aac ttg gct gtc 1027 Ala Val Ala Ala Ala Gln Gly Ile Gln Ala Thr Val Asn Leu Ala Val     295 300 305 atc gga agc ccc ggt gga tca tcg ctg ctt tct acc gtg cag gct ttc 1075 Ile Gly Ser Pro Gly Gly Ser Ser Leu Leu Ser Thr Val Gln Ala Phe 310 315 320 325 cga ttc ttc gga tca gcg gca gca cca gtg aca ttc ctt cct atc tat 1123 Arg Phe Phe Gly Ser Ala Ala Ala Pro Val Thr Phe Leu Pro Ile Tyr                 330 335 340 atg ggc atc ggc tcg ggg gcg ttt tgg gtc agc gcg gta gcg ctg ttc 1171 Met Gly Ile Gly Ser Gly Ala Phe Trp Val Ser Ala Val Ala Leu Phe             345 350 355 ttc gtt gcc atc gcc cag tgg ctc aac ccg cag cgg gtg gag cgg ggc 1219 Phe Val Ala Ile Ala Gln Trp Leu Asn Pro Gln Arg Val Glu Arg Gly         360 365 370 tgagggagac gtcgagaagc gtc 1242 <210> 174 <211> 373 <212> PRT <213> Corynebacterium glutamicum <400> 174 Met Lys Lys Leu Gln Met Pro Ala Ile Leu Val Gly Gly Phe Val Gly   1 5 10 15 Pro Phe Thr Gly Gln Ala Leu Ser Val Val Leu Pro Glu Phe Ala Asp              20 25 30 Thr Phe Asp Ile Ser Val Ser Gln Ala Ala Leu Thr Met Thr Ala Tyr          35 40 45 Leu Leu Pro Phe Ala Thr Met Met Leu Phe Ser Gly Arg Ile Thr Arg      50 55 60 Lys Ile His Pro His Lys Val Val Gln Ala Ala Tyr Ile Val Thr Leu  65 70 75 80 Pro Leu Ala Leu Leu Leu Leu Val Thr Pro Ser Trp Gly Leu Phe Met                  85 90 95 Ala Ala Tyr Ala Thr Ile Gly Ile Ala Asn Ala Phe Thr Thr Pro Val             100 105 110 Leu Gln Ile Met Leu Arg Glu Leu Val Pro Pro Arg Ser Leu Gly Lys         115 120 125 Ala Leu Gly Thr Tyr Ala Ala Met Gln Ser Leu Gly Met Leu Ser Ala     130 135 140 Pro Leu Ile Ala Gly Val Ser Ser Val Val Ser Trp Arg Leu Thr Phe 145 150 155 160 Leu Val Thr Ala Ala Ala Ser Leu Phe Ile Leu Val Ala Arg Leu Pro                 165 170 175 Val Val Pro Pro Pro Ser Ala Ser Lys Gln Asn Val Ser Gly Lys Val             180 185 190 Gln Trp Gly Pro Thr Ile Ile His Met Val Ser Gly Phe Val Val Gly         195 200 205 Ile Gly Ile Ile Gly Ile Gly Phe Met Thr Ser Leu His Val Gly Glu     210 215 220 Gln Phe Gly Leu Asp Ala Ala Ala Arg Gly Leu Val Val Met Cys Gly 225 230 235 240 Gly Leu Ala Ala Phe Phe Ala Ser Arg Lys Ile Gly Asp Leu Ala Asp                 245 250 255 Lys Phe Gly Val Arg Ala Val Leu Ile Val Ser Ala Val Ile Gly Thr             260 265 270 Ile Ala Leu Ala Leu Leu Pro Ile Ala Pro Trp Ile Ile Val Val Ala         275 280 285 Val Leu Trp Ala Phe Ala Val Ala Ala Ala Gln Gly Ile Gln Ala Thr     290 295 300 Val Asn Leu Ala Val Ile Gly Ser Pro Gly Gly Ser Ser Leu Leu Ser 305 310 315 320 Thr Val Gln Ala Phe Arg Phe Phe Gly Ser Ala Ala Ala Pro Val Thr                 325 330 335 Phe Leu Pro Ile Tyr Met Gly Ile Gly Ser Gly Ala Phe Trp Val Ser             340 345 350 Ala Val Ala Leu Phe Phe Val Ala Ile Ala Gln Trp Leu Asn Pro Gln         355 360 365 Arg Val Glu Arg Gly     370 <175> 175 <211> 871 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (871) <223> FRXA01010 <400> 175 gtgccaaagc gtttcctgta aaacgcataa ccccgaatac cccctgtttc cagatccaaa 60 aaaagatctg gcagggggtt taggcataga ttaggaactt atg aag aaa ctg caa 115                                             Met Lys Lys Leu Gln                                               1 5 atg ccg gcc att ttg gtc gga ggc ttt gtg ggg ccg ttt act ggc caa 163 Met Pro Ala Ile Leu Val Gly Gly Phe Val Gly Pro Phe Thr Gly Gln                  10 15 20 gct cta tca gtg gtc ttg ccg gaa ttt gca gac acc ttt gat atc agt 211 Ala Leu Ser Val Val Leu Pro Glu Phe Ala Asp Thr Phe Asp Ile Ser              25 30 35 gtc agc cag gca gcg ctg acc atg acc gca tac ttg ttg ccc ttt gcc 259 Val Ser Gln Ala Ala Leu Thr Met Thr Ala Tyr Leu Leu Pro Phe Ala          40 45 50 acc atg atg ttg ttt tcg ggg cgc atc acc aga aag atc cat ccg cat 307 Thr Met Met Leu Phe Ser Gly Arg Ile Thr Arg Lys Ile His Pro His      55 60 65 aag gtg gtg cag gcg gct tat att gtc aca ctg cca ctt gcg ctg ttg 355 Lys Val Val Gln Ala Ala Tyr Ile Val Thr Leu Pro Leu Ala Leu Leu  70 75 80 85 ctc cta gtt aca cca tcg tgg ggg ctg ttt atg gct gcg tat gcc acg 403 Leu Leu Val Thr Pro Ser Trp Gly Leu Phe Met Ala Ala Tyr Ala Thr                  90 95 100 att ggt atc gct aat gca ttt acc act ccg gtg ctg caa att atg ttg 451 Ile Gly Ile Ala Asn Ala Phe Thr Thr Pro Val Leu Gln Ile Met Leu             105 110 115 cgt gag ctt gtt ccg ccg cgt tct ttg ggt aag gca ttg ggc acc tat 499 Arg Glu Leu Val Pro Pro Arg Ser Leu Gly Lys Ala Leu Gly Thr Tyr         120 125 130 gct gcg atg caa tca ctc ggc atg ttg tcg gcg cca ctg atc gca ggt 547 Ala Ala Met Gln Ser Leu Gly Met Leu Ser Ala Pro Leu Ile Ala Gly     135 140 145 gtg tct tcg gtg gtg tcg tgg agg ttg acc ttc ctg gtc act gca gca 595 Val Ser Ser Val Val Ser Trp Arg Leu Thr Phe Leu Val Thr Ala Ala 150 155 160 165 gcg tca ctg ttt att ttg gtg gcg cga ctc ccc gtt gtt cca cca cca 643 Ala Ser Leu Phe Ile Leu Val Ala Arg Leu Pro Val Val Pro Pro Pro                 170 175 180 tca gca ttg aag caa aac gtt agt ggc aag gtg cag tgg gga ccg acc 691 Ser Ala Leu Lys Gln Asn Val Ser Gly Lys Val Gln Trp Gly Pro Thr             185 190 195 atc atc cac atg gtt tcc ggc ttt gtg gtg ggc atc ggc atc atc ggc 739 Ile Ile His Met Val Ser Gly Phe Val Val Gly Ile Gly Ile Ile Gly         200 205 210 att gga ttc atg aca tcg ctg cac gtt ggc gag caa ttc gga ctt aat 787 Ile Gly Phe Met Thr Ser Leu His Val Gly Glu Gln Phe Gly Leu Asn     215 220 225 act gca gcg cgt ggt ttg gtg gtc atg tgt ggt ggc cgg gct gcg ttc 835 Thr Ala Ala Arg Gly Leu Val Val Met Cys Gly Gly Arg Ala Ala Phe 230 235 240 245 ttt gcc tcc cgc aag ata ggc gat ttg gca gac aaa 871 Phe Ala Ser Arg Lys Ile Gly Asp Leu Ala Asp Lys                 250 255 <210> 176 <211> 257 <212> PRT <213> Corynebacterium glutamicum <400> 176 Met Lys Lys Leu Gln Met Pro Ala Ile Leu Val Gly Gly Phe Val Gly   1 5 10 15 Pro Phe Thr Gly Gln Ala Leu Ser Val Val Leu Pro Glu Phe Ala Asp              20 25 30 Thr Phe Asp Ile Ser Val Ser Gln Ala Ala Leu Thr Met Thr Ala Tyr          35 40 45 Leu Leu Pro Phe Ala Thr Met Met Leu Phe Ser Gly Arg Ile Thr Arg      50 55 60 Lys Ile His Pro His Lys Val Val Gln Ala Ala Tyr Ile Val Thr Leu  65 70 75 80 Pro Leu Ala Leu Leu Leu Leu Val Thr Pro Ser Trp Gly Leu Phe Met                  85 90 95 Ala Ala Tyr Ala Thr Ile Gly Ile Ala Asn Ala Phe Thr Thr Pro Val             100 105 110 Leu Gln Ile Met Leu Arg Glu Leu Val Pro Pro Arg Ser Leu Gly Lys         115 120 125 Ala Leu Gly Thr Tyr Ala Ala Met Gln Ser Leu Gly Met Leu Ser Ala     130 135 140 Pro Leu Ile Ala Gly Val Ser Ser Val Val Ser Trp Arg Leu Thr Phe 145 150 155 160 Leu Val Thr Ala Ala Ala Ser Leu Phe Ile Leu Val Ala Arg Leu Pro                 165 170 175 Val Val Pro Pro Pro Ser Ala Leu Lys Gln Asn Val Ser Gly Lys Val             180 185 190 Gln Trp Gly Pro Thr Ile Ile His Met Val Ser Gly Phe Val Val Gly         195 200 205 Ile Gly Ile Ile Gly Ile Gly Phe Met Thr Ser Leu His Val Gly Glu     210 215 220 Gln Phe Gly Leu Asn Thr Ala Ala Arg Gly Leu Val Val Met Cys Gly 225 230 235 240 Gly Arg Ala Ala Phe Phe Ala Ser Arg Lys Ile Gly Asp Leu Ala Asp                 245 250 255 Lys      <210> 177 <211> 1266 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (1243) <223> RXN03142 <400> 177 gcaatttggc caatcaacaa cataggagga ctgcgtggcg gtcaccgcaa gaacggacat 60 aaaaccacac ccatcgcaac caacggcact gttcactcca gtg ttt att ttg ggc 115                                             Val Phe Ile Leu Gly                                               1 5 tgg ctc gtc aac ttg acc cag tac ttg agc ttc tac ttc ctg atc aca 163 Trp Leu Val Asn Leu Thr Gln Tyr Leu Ser Phe Tyr Phe Leu Ile Thr                  10 15 20 gtc atg gcg ctg tat gcg atg gaa agc ttc gcc gtt tca gag gcc gct 211 Val Met Ala Leu Tyr Ala Met Glu Ser Phe Ala Val Ser Glu Ala Ala              25 30 35 gtc gga ttt gcg gcc agc tcc ttt gtt atc ggc gca acc gtg gct cgt 259 Val Gly Phe Ala Ala Ser Ser Phe Val Ile Gly Ala Thr Val Ala Arg          40 45 50 gtg ttc gcg gga tgg acg tcc gac cgt ttt ggt aaa aaa cag atc ctg 307 Val Phe Ala Gly Trp Thr Ser Asp Arg Phe Gly Lys Lys Gln Ile Leu      55 60 65 ctc atc ttt gtc ggc ttg gaa gcg gta gca tca cta ttc tat att cca 355 Leu Ile Phe Val Gly Leu Glu Ala Val Ala Ser Leu Phe Tyr Ile Pro  70 75 80 85 gct gcc tca cta cca gcg ctg gtt gct gtg cgt ttt gtt cac ggt ttt 403 Ala Ala Ser Leu Pro Ala Leu Val Ala Val Arg Phe Val His Gly Phe                  90 95 100 tct tat tct ctt gct tcc acc gct gtg atg gca ctt gtg cag tcc gtg 451 Ser Tyr Ser Leu Ala Ser Thr Ala Val Met Ala Leu Val Gln Ser Val             105 110 115 att cct gca agc cgt agg gca gag ggc acc ggc tac ttc gcg ctc gga 499 Ile Pro Ala Ser Arg Arg Ala Glu Gly Thr Gly Tyr Phe Ala Leu Gly         120 125 130 tcc aca ctg gct aca gct ttc ggc cca gca att gcg ctg ttt gtt atc 547 Ser Thr Leu Ala Thr Ala Phe Gly Pro Ala Ile Ala Leu Phe Val Ile     135 140 145 gat gac ttc aac tac aac acc ctg ttc tgg att acc act gcg acc agt 595 Asp Asp Phe Asn Tyr Asn Thr Leu Phe Trp Ile Thr Thr Ala Thr Ser 150 155 160 165 gtt ttc ggc ctg atc ctc acc gtt ttg atc cgc aag ccg gag ttc att 643 Val Phe Gly Leu Ile Leu Thr Val Leu Ile Arg Lys Pro Glu Phe Ile                 170 175 180 aag aat gcg gaa cac ggc aga gta aag cca gtc tgg tct atc aag act 691 Lys Asn Ala Glu His Gly Arg Val Lys Pro Val Trp Ser Ile Lys Thr             185 190 195 gtt gtg cac cca tcg gtc atg ctc att gga ttc ttc atg ctc gct gtc 739 Val Val His Pro Ser Val Met Leu Ile Gly Phe Phe Met Leu Ala Val         200 205 210 gga ctg gct tac gca ggc gtg atc acc ttc ctc aac ggc ttc gcg caa 787 Gly Leu Ala Tyr Ala Gly Val Ile Thr Phe Leu Asn Gly Phe Ala Gln     215 220 225 gac act ggc ctc acc gcc gga gcg ggt ctt ttc ttt atc gct tat gcg 835 Asp Thr Gly Leu Thr Ala Gly Ala Gly Leu Phe Phe Ile Ala Tyr Ala 230 235 240 245 gtt gcg atg ctg gtc atg cgt ttc ttc ctt gga cgc att cag gac aaa 883 Val Ala Met Leu Val Met Arg Phe Phe Leu Gly Arg Ile Gln Asp Lys                 250 255 260 cat ggt gac aac ccg gtt att tac ttc ggt ttg atc agc ttc gcc ctc 931 His Gly Asp Asn Pro Val Ile Tyr Phe Gly Leu Ile Ser Phe Ala Leu             265 270 275 gcg ctg ggg ctt atg gct ttg gcg act gaa gac tgg cac att gtt ctc 979 Ala Leu Gly Leu Met Ala Leu Ala Thr Glu Asp Trp His Ile Val Leu         280 285 290 gct ggc gca ctc acc ggt ttg ggc tat ggc acc atc atg ccg gcc gca 1027 Ala Gly Ala Leu Thr Gly Leu Gly Tyr Gly Thr Ile Met Pro Ala Ala     295 300 305 caa gcc att gct gtc gat tca gtt cca agc act cag gtt ggt tcc ggt 1075 Gln Ala Ile Ala Val Asp Ser Val Pro Ser Thr Gln Val Gly Ser Gly 310 315 320 325 att tct acg ctt ttc ctg ttc acc gac atc ggc att ggc tta ggc cca 1123 Ile Ser Thr Leu Phe Leu Phe Thr Asp Ile Gly Ile Gly Leu Gly Pro                 330 335 340 atc ctg ctg ggt gga ttg gtt gca gcg acc gga tac aac gtc atg tac 1171 Ile Leu Leu Gly Gly Leu Val Ala Ala Thr Gly Tyr Asn Val Met Tyr             345 350 355 gca gct ttg gcc gca gtg att gtt gtg gcg ggc gtg ctc tac ctg gtt 1219 Ala Ala Leu Ala Ala Val Ile Val Val Ala Gly Val Leu Tyr Leu Val         360 365 370 gct ttg ggt agg aaa gct agc cac taagttagag cattttattg agc 1266 Ala Leu Gly Arg Lys Ala Ser His     375 380 <210> 178 <211> 381 <212> PRT <213> Corynebacterium glutamicum <400> 178 Val Phe Ile Leu Gly Trp Leu Val Asn Leu Thr Gln Tyr Leu Ser Phe   1 5 10 15 Tyr Phe Leu Ile Thr Val Met Ala Leu Tyr Ala Met Glu Ser Phe Ala              20 25 30 Val Ser Glu Ala Ala Val Gly Phe Ala Ala Ser Ser Phe Val Ile Gly          35 40 45 Ala Thr Val Ala Arg Val Phe Ala Gly Trp Thr Ser Asp Arg Phe Gly      50 55 60 Lys Lys Gln Ile Leu Leu Ile Phe Val Gly Leu Glu Ala Val Ala Ser  65 70 75 80 Leu Phe Tyr Ile Pro Ala Ala Ser Leu Pro Ala Leu Val Ala Val Arg                  85 90 95 Phe Val His Gly Phe Ser Tyr Ser Leu Ala Ser Thr Ala Val Met Ala             100 105 110 Leu Val Gln Ser Val Ile Pro Ala Ser Arg Arg Ala Glu Gly Thr Gly         115 120 125 Tyr Phe Ala Leu Gly Ser Thr Leu Ala Thr Ala Phe Gly Pro Ala Ile     130 135 140 Ala Leu Phe Val Ile Asp Asp Phe Asn Tyr Asn Thr Leu Phe Trp Ile 145 150 155 160 Thr Thr Ala Thr Ser Val Phe Gly Leu Ile Leu Thr Val Leu Ile Arg                 165 170 175 Lys Pro Glu Phe Ile Lys Asn Ala Glu His Gly Arg Val Lys Pro Val             180 185 190 Trp Ser Ile Lys Thr Val Val His Pro Ser Val Met Leu Ile Gly Phe         195 200 205 Phe Met Leu Ala Val Gly Leu Ala Tyr Ala Gly Val Ile Thr Phe Leu     210 215 220 Asn Gly Phe Ala Gln Asp Thr Gly Leu Thr Ala Gly Ala Gly Leu Phe 225 230 235 240 Phe Ile Ala Tyr Ala Val Ala Met Leu Val Met Arg Phe Phe Leu Gly                 245 250 255 Arg Ile Gln Asp Lys His Gly Asp Asn Pro Val Ile Tyr Phe Gly Leu             260 265 270 Ile Ser Phe Ala Leu Ala Leu Gly Leu Met Ala Leu Ala Thr Glu Asp         275 280 285 Trp His Ile Val Leu Ala Gly Ala Leu Thr Gly Leu Gly Tyr Gly Thr     290 295 300 Ile Met Pro Ala Ala Gln Ala Ile Ala Val Asp Ser Val Pro Ser Thr 305 310 315 320 Gln Val Gly Ser Gly Ile Ser Thr Leu Phe Leu Phe Thr Asp Ile Gly                 325 330 335 Ile Gly Leu Gly Pro Ile Leu Leu Gly Gly Leu Val Ala Ala Thr Gly             340 345 350 Tyr Asn Val Met Tyr Ala Ala Leu Ala Ala Val Ile Val Val Ala Gly         355 360 365 Val Leu Tyr Leu Val Ala Leu Gly Arg Lys Ala Ser His     370 375 380 <210> 179 <211> 914 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (1) .. (891) <223> FRXA01150 <400> 179 cca gct gcc tca cta cca gcg ctg gtt gct gtg cgt ttt gtt cac ggt 48 Pro Ala Ala Ser Leu Pro Ala Leu Val Ala Val Arg Phe Val His Gly   1 5 10 15 ttt tct tat tct ctt gct tcc acc gct gtg atg gca ctt gtg cag tcc 96 Phe Ser Tyr Ser Leu Ala Ser Thr Ala Val Met Ala Leu Val Gln Ser              20 25 30 gtg att cct gca agc cgt agg gca gag ggc acc ggc tac ttc gcg ctc 144 Val Ile Pro Ala Ser Arg Arg Ala Glu Gly Thr Gly Tyr Phe Ala Leu          35 40 45 gga tcc aca ctg gct aca gct ttc ggc cca gca att gcg ctg ttt gtt 192 Gly Ser Thr Leu Ala Thr Ala Phe Gly Pro Ala Ile Ala Leu Phe Val      50 55 60 atc gat gac ttc aac tac aac acc ctg ttc tgg att acc act gcg acc 240 Ile Asp Asp Phe Asn Tyr Asn Thr Leu Phe Trp Ile Thr Thr Ala Thr  65 70 75 80 agt gtt ttc ggc ctg atc ctc acc gtt ttg atc cgc aag ccg gag ttc 288 Ser Val Phe Gly Leu Ile Leu Thr Val Leu Ile Arg Lys Pro Glu Phe                  85 90 95 att aag aat gcg gaa cac ggc aga gta aag cca gtc tgg tct atc aag 336 Ile Lys Asn Ala Glu His Gly Arg Val Lys Pro Val Trp Ser Ile Lys             100 105 110 act gtt gtg cac cca tcg gtc atg ctc att gga ttc ttc atg ctc gct 384 Thr Val Val His Pro Ser Val Met Leu Ile Gly Phe Phe Met Leu Ala         115 120 125 gtc gga ctg gct tac gca ggc gtg atc acc ttc ctc aac ggc ttc gcg 432 Val Gly Leu Ala Tyr Ala Gly Val Ile Thr Phe Leu Asn Gly Phe Ala     130 135 140 caa gac act ggc ctc acc gcc gga gcg ggt ctt ttc ttt atc gct tat 480 Gln Asp Thr Gly Leu Thr Ala Gly Ala Gly Leu Phe Phe Ile Ala Tyr 145 150 155 160 gcg gtt gcg atg ctg gtc atg cgt ttc ttc ctt gga cgc att cag gac 528 Ala Val Ala Met Leu Val Met Arg Phe Phe Leu Gly Arg Ile Gln Asp                 165 170 175 aaa cat ggt gac aac ccg gtt att tac ttc ggt ttg atc agc ttc gcc 576 Lys His Gly Asp Asn Pro Val Ile Tyr Phe Gly Leu Ile Ser Phe Ala             180 185 190 ctc gcg ctg ggg ctt atg gct ttg gcg act gaa gac tgg cac att gtt 624 Leu Ala Leu Gly Leu Met Ala Leu Ala Thr Glu Asp Trp His Ile Val         195 200 205 ctc gct ggc gca ctc acc ggt ttg ggc tat ggc acc atc atg ccg gcc 672 Leu Ala Gly Ala Leu Thr Gly Leu Gly Tyr Gly Thr Ile Met Pro Ala     210 215 220 gca caa gcc att gct gtc gat tca gtt cca agc act cag gtt ggt tcc 720 Ala Gln Ala Ile Ala Val Asp Ser Val Pro Ser Thr Gln Val Gly Ser 225 230 235 240 ggt att tct acg ctt ttc ctg ttc acc gac atc ggc att ggc tta ggc 768 Gly Ile Ser Thr Leu Phe Leu Phe Thr Asp Ile Gly Ile Gly Leu Gly                 245 250 255 cca atc ctg ctg ggt gga ttg gtt gca gcg acc gga tac aac gtc atg 816 Pro Ile Leu Leu Gly Gly Leu Val Ala Ala Thr Gly Tyr Asn Val Met             260 265 270 tac gca gct ttg gcc gca gtg att gtt gtg gcg ggc gtg ctc tac ctg 864 Tyr Ala Ala Leu Ala Ala Val Ile Val Val Ala Gly Val Leu Tyr Leu         275 280 285 gtt gct ttg ggt agg aaa gct agc cac taagttagag cattttattg 911 Val Ala Leu Gly Arg Lys Ala Ser His     290 295 agc 914 <210> 180 <211> 297 <212> PRT <213> Corynebacterium glutamicum <400> 180 Pro Ala Ala Ser Leu Pro Ala Leu Val Ala Val Arg Phe Val His Gly   1 5 10 15 Phe Ser Tyr Ser Leu Ala Ser Thr Ala Val Met Ala Leu Val Gln Ser              20 25 30 Val Ile Pro Ala Ser Arg Arg Ala Glu Gly Thr Gly Tyr Phe Ala Leu          35 40 45 Gly Ser Thr Leu Ala Thr Ala Phe Gly Pro Ala Ile Ala Leu Phe Val      50 55 60 Ile Asp Asp Phe Asn Tyr Asn Thr Leu Phe Trp Ile Thr Thr Ala Thr  65 70 75 80 Ser Val Phe Gly Leu Ile Leu Thr Val Leu Ile Arg Lys Pro Glu Phe                  85 90 95 Ile Lys Asn Ala Glu His Gly Arg Val Lys Pro Val Trp Ser Ile Lys             100 105 110 Thr Val Val His Pro Ser Val Met Leu Ile Gly Phe Phe Met Leu Ala         115 120 125 Val Gly Leu Ala Tyr Ala Gly Val Ile Thr Phe Leu Asn Gly Phe Ala     130 135 140 Gln Asp Thr Gly Leu Thr Ala Gly Ala Gly Leu Phe Phe Ile Ala Tyr 145 150 155 160 Ala Val Ala Met Leu Val Met Arg Phe Phe Leu Gly Arg Ile Gln Asp                 165 170 175 Lys His Gly Asp Asn Pro Val Ile Tyr Phe Gly Leu Ile Ser Phe Ala             180 185 190 Leu Ala Leu Gly Leu Met Ala Leu Ala Thr Glu Asp Trp His Ile Val         195 200 205 Leu Ala Gly Ala Leu Thr Gly Leu Gly Tyr Gly Thr Ile Met Pro Ala     210 215 220 Ala Gln Ala Ile Ala Val Asp Ser Val Pro Ser Thr Gln Val Gly Ser 225 230 235 240 Gly Ile Ser Thr Leu Phe Leu Phe Thr Asp Ile Gly Ile Gly Leu Gly                 245 250 255 Pro Ile Leu Leu Gly Gly Leu Val Ala Ala Thr Gly Tyr Asn Val Met             260 265 270 Tyr Ala Ala Leu Ala Ala Val Ile Val Val Ala Gly Val Leu Tyr Leu         275 280 285 Val Ala Leu Gly Arg Lys Ala Ser His     290 295 <210> 181 <211> 1341 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (101) .. (1318) <223> RXN02964 <400> 181 ttttatatcc tagcaagggt gttgcatgat gcaataaacg tggtagtttg tgttcataac 60 aaaattgcat gatgcaataa tttcgattta aaggagaaca gtg tcc gta gct gaa 115                                             Val Ser Val Ala Glu                                               1 5 gaa ggg aaa ctt ttt aca cca acg ttt gtc atg gga tgg ttt gcc aac 163 Glu Gly Lys Leu Phe Thr Pro Thr Phe Val Met Gly Trp Phe Ala Asn                  10 15 20 ctt ttc cag ttc ctg gtg ttc tac ttc ctc atc acc acc atg gct ttg 211 Leu Phe Gln Phe Leu Val Phe Tyr Phe Leu Ile Thr Thr Met Ala Leu              25 30 35 tac gcc atc aag gaa ttt caa gcc tct gaa gta gaa gct ggc ttc gca 259 Tyr Ala Ile Lys Glu Phe Gln Ala Ser Glu Val Glu Ala Gly Phe Ala          40 45 50 tcc agc tca att gtt atc ggc gca gtc ttt tcc agg ttt ttc tcc ggc 307 Ser Ser Ser Ile Val Ile Gly Ala Val Phe Ser Arg Phe Phe Ser Gly      55 60 65 tat att att gac cgt ttt ggt cga cgc aag att gtg ctc atc tca gtc 355 Tyr Ile Ile Asp Arg Phe Gly Arg Arg Lys Ile Val Leu Ile Ser Val  70 75 80 85 cta gtc act acc att gcg tgt gcc ttg tac ctt ccc atc gaa tca ttg 403 Leu Val Thr Thr Ile Ala Cys Ala Leu Tyr Leu Pro Ile Glu Ser Leu                  90 95 100 cca ttg cta tac gca aac agg ttc ctc cac ggt gtt gga tac gct ttt 451 Pro Leu Leu Tyr Ala Asn Arg Phe Leu His Gly Val Gly Tyr Ala Phe             105 110 115 gct gcc acc gcg atc atg gca atg gtc cag gag ctc att cca gcg tca 499 Ala Ala Thr Ala Ile Met Ala Met Val Gln Glu Leu Ile Pro Ala Ser         120 125 130 cga cgt tcc gaa ggt act ggt tac ctg gca ttg ggc act acc gtt tct 547 Arg Arg Ser Glu Gly Thr Gly Tyr Leu Ala Leu Gly Thr Thr Val Ser     135 140 145 gca gca ctt gga cca gcc cta gca ctt ttt gtc cta gga aca ttt gat 595 Ala Ala Leu Gly Pro Ala Leu Ala Leu Phe Val Leu Gly Thr Phe Asp 150 155 160 165 tac gac atg ctg ttt atc gtg gtc ttg gca acc tcg gtc atc tct ttg 643 Tyr Asp Met Leu Phe Ile Val Val Leu Ala Thr Ser Val Ile Ser Leu                 170 175 180 atc gcc gtc gtg ttc atg tac ttt aag acc agc gac cct gag cct tct 691 Ile Ala Val Val Phe Met Tyr Phe Lys Thr Ser Asp Pro Glu Pro Ser             185 190 195 ggg gaa cca gcc aag ttc agc ttc aaa tct att atg aac cca aag atc 739 Gly Glu Pro Ala Lys Phe Ser Phe Lys Ser Ile Met Asn Pro Lys Ile         200 205 210 atc ccc atc ggc atc ttt atc ttg ctt att tgc ttt gct tac tct ggc 787 Ile Pro Ile Gly Ile Phe Ile Leu Leu Ile Cys Phe Ala Tyr Ser Gly     215 220 225 gtc att gcc tac atc aac gca ttt gct gaa gaa cgc gat ctg att acg 835 Val Ile Ala Tyr Ile Asn Ala Phe Ala Glu Glu Arg Asp Leu Ile Thr 230 235 240 245 ggt gct gga ttg ttc ttc att gcc tac gca gta tca atg ttt gtg atg 883 Gly Ala Gly Leu Phe Phe Ile Ala Tyr Ala Val Ser Met Phe Val Met                 250 255 260 cgc agc ttc ctt ggc aaa ctg cag gac cgt cgc gga gac aac gtc gtt 931 Arg Ser Phe Leu Gly Lys Leu Gln Asp Arg Arg Gly Asp Asn Val Val             265 270 275 att tac ttt gga ttg ttc ttc ttc gtt att tcc ttg acg att ttg tcc 979 Ile Tyr Phe Gly Leu Phe Phe Phe Val Ile Ser Leu Thr Ile Leu Ser         280 285 290 ttt gcc act tcc aac tgg cac gtt gtg ttg tcc gga gtc att gca ggt 1027 Phe Ala Thr Ser Asn Trp His Val Val Leu Ser Gly Val Ile Ala Gly     295 300 305 ctg gga tac ggc act ttg atg cca gca gtg cag tcc atc gct gtt ggt 1075 Leu Gly Tyr Gly Thr Leu Met Pro Ala Val Gln Ser Ile Ala Val Gly 310 315 320 325 gta gta gac aaa acc gaa ttc ggt acg gcc ttc tcc act ttg ttc ctg 1123 Val Val Asp Lys Thr Glu Phe Gly Thr Ala Phe Ser Thr Leu Phe Leu                 330 335 340 ttt gtg gac tta ggt ttt ggc ttt gga cct att atc ctg gga gca gtt 1171 Phe Val Asp Leu Gly Phe Gly Phe Gly Pro Ile Ile Leu Gly Ala Val             345 350 355 tct gcg gca att ggt ttc gga cct atg tat gca gca ctg gca ggt gtg 1219 Ser Ala Ala Ile Gly Phe Gly Pro Met Tyr Ala Ala Leu Ala Gly Val         360 365 370 ggt gtg att gcc gga atc ttc tac ctg ttc aca cac gct cgc acc gat 1267 Gly Val Ile Ala Gly Ile Phe Tyr Leu Phe Thr His Ala Arg Thr Asp     375 380 385 cga gct aag aat ggc ttt gtt aaa cac cca gag cct gtc gct tta gtt 1315 Arg Ala Lys Asn Gly Phe Val Lys His Pro Glu Pro Val Ala Leu Val 390 395 400 405 agc tagttctttc agctttccct ccc 1341 Ser      <210> 182 <211> 406 <212> PRT <213> Corynebacterium glutamicum <400> 182 Val Ser Val Ala Glu Glu Gly Lys Leu Phe Thr Pro Thr Phe Val Met   1 5 10 15 Gly Trp Phe Ala Asn Leu Phe Gln Phe Leu Val Phe Tyr Phe Leu Ile              20 25 30 Thr Thr Met Ala Leu Tyr Ala Ile Lys Glu Phe Gln Ala Ser Glu Val          35 40 45 Glu Ala Gly Phe Ala Ser Ser Ser Ile Val Ile Gly Ala Val Phe Ser      50 55 60 Arg Phe Phe Ser Gly Tyr Ile Ile Asp Arg Phe Gly Arg Arg Lys Ile  65 70 75 80 Val Leu Ile Ser Val Leu Val Thr Thr Ile Ala Cys Ala Leu Tyr Leu                  85 90 95 Pro Ile Glu Ser Leu Pro Leu Leu Tyr Ala Asn Arg Phe Leu His Gly             100 105 110 Val Gly Tyr Ala Phe Ala Ala Thr Ala Ile Met Ala Met Val Gln Glu         115 120 125 Leu Ile Pro Ala Ser Arg Arg Ser Glu Gly Thr Gly Tyr Leu Ala Leu     130 135 140 Gly Thr Thr Val Ser Ala Ala Leu Gly Pro Ala Leu Ala Leu Phe Val 145 150 155 160 Leu Gly Thr Phe Asp Tyr Asp Met Leu Phe Ile Val Val Leu Ala Thr                 165 170 175 Ser Val Ile Ser Leu Ile Ala Val Val Phe Met Tyr Phe Lys Thr Ser             180 185 190 Asp Pro Glu Pro Ser Gly Glu Pro Ala Lys Phe Ser Phe Lys Ser Ile         195 200 205 Met Asn Pro Lys Ile Ile Pro Ile Gly Ile Phe Ile Leu Leu Ile Cys     210 215 220 Phe Ala Tyr Ser Gly Val Ile Ala Tyr Ile Asn Ala Phe Ala Glu Glu 225 230 235 240 Arg Asp Leu Ile Thr Gly Ala Gly Leu Phe Phe Ile Ala Tyr Ala Val                 245 250 255 Ser Met Phe Val Met Arg Ser Phe Leu Gly Lys Leu Gln Asp Arg Arg             260 265 270 Gly Asp Asn Val Val Ile Tyr Phe Gly Leu Phe Phe Phe Val Ile Ser         275 280 285 Leu Thr Ile Leu Ser Phe Ala Thr Ser Asn Trp His Val Val Leu Ser     290 295 300 Gly Val Ile Ala Gly Leu Gly Tyr Gly Thr Leu Met Pro Ala Val Gln 305 310 315 320 Ser Ile Ala Val Gly Val Val Asp Lys Thr Glu Phe Gly Thr Ala Phe                 325 330 335 Ser Thr Leu Phe Leu Phe Val Asp Leu Gly Phe Gly Phe Gly Pro Ile             340 345 350 Ile Leu Gly Ala Val Ser Ala Ala Ile Gly Phe Gly Pro Met Tyr Ala         355 360 365 Ala Leu Ala Gly Val Gly Val Ile Ala Gly Ile Phe Tyr Leu Phe Thr     370 375 380 His Ala Arg Thr Asp Arg Ala Lys Asn Gly Phe Val Lys His Pro Glu 385 390 395 400 Pro Val Ala Leu Val Ser                 405 <210> 183 <211> 1006 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (1006) <223> FRXA02116 <400> 183 ttttatatcc tagcaagggt gttgcatgat gcaataaacg tggtagtttg tgttcataac 60 aaaattgcat gatgcaataa tttcgattta aaggagaaca gtg tcc gta gct gaa 115                                             Val Ser Val Ala Glu                                               1 5 gaa ggg aaa ctt ttt aca cca acg ttt gtc atg gga tgg ttt gcc aac 163 Glu Gly Lys Leu Phe Thr Pro Thr Phe Val Met Gly Trp Phe Ala Asn                  10 15 20 ctt ttc cag ttc ctg gtg ttc tac ttc ctc atc acc acc atg gct ttg 211 Leu Phe Gln Phe Leu Val Phe Tyr Phe Leu Ile Thr Thr Met Ala Leu              25 30 35 tac gcc atc aag gaa ttt caa gcc tct gaa gta gaa gct ggc ttc gca 259 Tyr Ala Ile Lys Glu Phe Gln Ala Ser Glu Val Glu Ala Gly Phe Ala          40 45 50 tcc agc tca att gtt atc ggc gca gtc ttt tcc agg ttt ttc tcc ggc 307 Ser Ser Ser Ile Val Ile Gly Ala Val Phe Ser Arg Phe Phe Ser Gly      55 60 65 tat att att gac cgt ttt ggt cga cgc aag att gtg ctc atc tca gtc 355 Tyr Ile Ile Asp Arg Phe Gly Arg Arg Lys Ile Val Leu Ile Ser Val  70 75 80 85 cta gtc act acc att gcg tgt gcc ttg tac ctt ccc atc gaa tca ttg 403 Leu Val Thr Thr Ile Ala Cys Ala Leu Tyr Leu Pro Ile Glu Ser Leu                  90 95 100 cca ttg cta tac gca aac agg ttc ctc cac ggt gtt gga tac gct ttt 451 Pro Leu Leu Tyr Ala Asn Arg Phe Leu His Gly Val Gly Tyr Ala Phe             105 110 115 gct gcc acc gcg atc atg gca atg gtc cag gag ctc att cca gcg tca 499 Ala Ala Thr Ala Ile Met Ala Met Val Gln Glu Leu Ile Pro Ala Ser         120 125 130 cga cgt tcc gaa ggt act ggt tac ctg gca ttg ggc act acc gtt tct 547 Arg Arg Ser Glu Gly Thr Gly Tyr Leu Ala Leu Gly Thr Thr Val Ser     135 140 145 gca gca ctt gga cca gcc cta gca ctt ttt gtc cta gga aca ttt gat 595 Ala Ala Leu Gly Pro Ala Leu Ala Leu Phe Val Leu Gly Thr Phe Asp 150 155 160 165 tac gac atg ctg ttt atc gtg gtc ttg gca acc tcg gtc atc tct ttg 643 Tyr Asp Met Leu Phe Ile Val Val Leu Ala Thr Ser Val Ile Ser Leu                 170 175 180 atc gcc gtc gtg ttc atg tac ttt aag acc agc gac cct gag cct tct 691 Ile Ala Val Val Phe Met Tyr Phe Lys Thr Ser Asp Pro Glu Pro Ser             185 190 195 ggg gaa cca gcc aag ttc agc ttc aaa tct att atg aac cca aag atc 739 Gly Glu Pro Ala Lys Phe Ser Phe Lys Ser Ile Met Asn Pro Lys Ile         200 205 210 atc ccc atc ggc atc ttt atc ttg ctt att tgc ttt gct tac tct ggc 787 Ile Pro Ile Gly Ile Phe Ile Leu Leu Ile Cys Phe Ala Tyr Ser Gly     215 220 225 gtc att gcc tac atc aac gca ttt gct gaa gaa cgc gat ctg att acg 835 Val Ile Ala Tyr Ile Asn Ala Phe Ala Glu Glu Arg Asp Leu Ile Thr 230 235 240 245 ggt gct gga ttg ttc ttc att gcc tac gca gta tca atg ttt gtg atg 883 Gly Ala Gly Leu Phe Phe Ile Ala Tyr Ala Val Ser Met Phe Val Met                 250 255 260 cgc agc ttc ctt ggc aaa ctg cag gac cgt cgc gga gac aac gtc gtt 931 Arg Ser Phe Leu Gly Lys Leu Gln Asp Arg Arg Gly Asp Asn Val Val             265 270 275 att tac ttt gga ttg ttc ttc ttc gtt att tcc ttg acg att ttg tcc 979 Ile Tyr Phe Gly Leu Phe Phe Phe Val Ile Ser Leu Thr Ile Leu Ser         280 285 290 ttt gcc act tcc aac tgg cac gtt gtg 1006 Phe Ala Thr Ser Asn Trp His Val Val     295 300 <210> 184 <211> 302 <212> PRT <213> Corynebacterium glutamicum <400> 184 Val Ser Val Ala Glu Glu Gly Lys Leu Phe Thr Pro Thr Phe Val Met   1 5 10 15 Gly Trp Phe Ala Asn Leu Phe Gln Phe Leu Val Phe Tyr Phe Leu Ile              20 25 30 Thr Thr Met Ala Leu Tyr Ala Ile Lys Glu Phe Gln Ala Ser Glu Val          35 40 45 Glu Ala Gly Phe Ala Ser Ser Ser Ile Val Ile Gly Ala Val Phe Ser      50 55 60 Arg Phe Phe Ser Gly Tyr Ile Ile Asp Arg Phe Gly Arg Arg Lys Ile  65 70 75 80 Val Leu Ile Ser Val Leu Val Thr Thr Ile Ala Cys Ala Leu Tyr Leu                  85 90 95 Pro Ile Glu Ser Leu Pro Leu Leu Tyr Ala Asn Arg Phe Leu His Gly             100 105 110 Val Gly Tyr Ala Phe Ala Ala Thr Ala Ile Met Ala Met Val Gln Glu         115 120 125 Leu Ile Pro Ala Ser Arg Arg Ser Glu Gly Thr Gly Tyr Leu Ala Leu     130 135 140 Gly Thr Thr Val Ser Ala Ala Leu Gly Pro Ala Leu Ala Leu Phe Val 145 150 155 160 Leu Gly Thr Phe Asp Tyr Asp Met Leu Phe Ile Val Val Leu Ala Thr                 165 170 175 Ser Val Ile Ser Leu Ile Ala Val Val Phe Met Tyr Phe Lys Thr Ser             180 185 190 Asp Pro Glu Pro Ser Gly Glu Pro Ala Lys Phe Ser Phe Lys Ser Ile         195 200 205 Met Asn Pro Lys Ile Ile Pro Ile Gly Ile Phe Ile Leu Leu Ile Cys     210 215 220 Phe Ala Tyr Ser Gly Val Ile Ala Tyr Ile Asn Ala Phe Ala Glu Glu 225 230 235 240 Arg Asp Leu Ile Thr Gly Ala Gly Leu Phe Phe Ile Ala Tyr Ala Val                 245 250 255 Ser Met Phe Val Met Arg Ser Phe Leu Gly Lys Leu Gln Asp Arg Arg             260 265 270 Gly Asp Asn Val Val Ile Tyr Phe Gly Leu Phe Phe Phe Val Ile Ser         275 280 285 Leu Thr Ile Leu Ser Phe Ala Thr Ser Asn Trp His Val Val     290 295 300 <210> 185 <211> 568 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101) .. (568) <223> RXA00858 <400> 185 ttttgttttt cagatgcatg ttagatgcgt tgagggacaa gggtggggga gacctccggt 60 tcttaaattg tctaaccaag aaccggaggt tctttttgtc atg gaa gta aac tta 115                                             Met glu val asn leu                                               1 5 gcc aca tgg cta atc act atc gca gtg att gct ggc ttc ttc att ttc 163 Ala Thr Trp Leu Ile Thr Ile Ala Val Ile Ala Gly Phe Phe Ile Phe                  10 15 20 gat ttc tat tcc cac gtc cgc acc cca cac gag ccc act atc aaa gaa 211 Asp Phe Tyr Ser His Val Arg Thr Pro His Glu Pro Thr Ile Lys Glu              25 30 35 tcc gca tgg tgg agc ctc ttc tac gta gcc ctc gcc tgt gtt ttc ggc 259 Ser Ala Trp Trp Ser Leu Phe Tyr Val Ala Leu Ala Cys Val Phe Gly          40 45 50 gtg ttc ctc tgg ttt gct tgg ggc gag cca ggt aac cca cac cag cac 307 Val Phe Leu Trp Phe Ala Trp Gly Glu Pro Gly Asn Pro His Gln His      55 60 65 ggc att gag ttc ttc acc ggt tac gtg aca gag aag gcg ttg agt gtt 355 Gly Ile Glu Phe Phe Thr Gly Tyr Val Thr Glu Lys Ala Leu Ser Val  70 75 80 85 gat aac ctc ttc atc ttc gcg ctg atc atg ggt tct ttc aag att cct 403 Asp Asn Leu Phe Ile Phe Ala Leu Ile Met Gly Ser Phe Lys Ile Pro                  90 95 100 cgc aag tac cag cag aag gtt ctg ctc atc ggt atc gcg ctg gca ctg 451 Arg Lys Tyr Gln Gln Lys Val Leu Leu Ile Gly Ile Ala Leu Ala Leu             105 110 115 gtc ttc cgc ctg gca ttc atc ctc gca ggt gct gca gtt atc gaa gcc 499 Val Phe Arg Leu Ala Phe Ile Leu Ala Gly Ala Ala Val Ile Glu Ala         120 125 130 tgg tcc gat gtc ttc tac atc ttc tcc atc tgg ctg atc tac acc gct 547 Trp Ser Asp Val Phe Tyr Ile Phe Ser Ile Trp Leu Ile Tyr Thr Ala     135 140 145 gtg aag gct cct gtg cac gag 568 Val Lys Ala Pro Val His Glu 150 155 <210> 186 <211> 156 <212> PRT <213> Corynebacterium glutamicum <400> 186 Met Glu Val Asn Leu Ala Thr Trp Leu Ile Thr Ile Ala Val Ile Ala   1 5 10 15 Gly Phe Phe Ile Phe Asp Phe Tyr Ser His Val Arg Thr Pro His Glu              20 25 30 Pro Thr Ile Lys Glu Ser Ala Trp Trp Ser Leu Phe Tyr Val Ala Leu          35 40 45 Ala Cys Val Phe Gly Val Phe Leu Trp Phe Ala Trp Gly Glu Pro Gly      50 55 60 Asn Pro His Gln His Gly Ile Glu Phe Phe Thr Gly Tyr Val Thr Glu  65 70 75 80 Lys Ala Leu Ser Val Asp Asn Leu Phe Ile Phe Ala Leu Ile Met Gly                  85 90 95 Ser Phe Lys Ile Pro Arg Lys Tyr Gln Gln Lys Val Leu Leu Ile Gly             100 105 110 Ile Ala Leu Ala Leu Val Phe Arg Leu Ala Phe Ile Leu Ala Gly Ala         115 120 125 Ala Val Ile Glu Ala Trp Ser Asp Val Phe Tyr Ile Phe Ser Ile Trp     130 135 140 Leu Ile Tyr Thr Ala Val Lys Ala Pro Val His Glu 145 150 155 <210> 187 <211> 975 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (952) <223> RXA02305 <400> 187 tatgcgcgca ggtgtctact ggtgacgcag ccgacgacga ttattttgac gaagccaccg 60 caaacgatga cttcgatccc gaaaagtgga ggaacatgta atg cca gcc ttt gag 115                                             Met Pro Ala Phe Glu                                               1 5 gca atg cca gga atg ccg tat tgg atc gac ctg tcc acc tcg gac att 163 Ala Met Pro Gly Met Pro Tyr Trp Ile Asp Leu Ser Thr Ser Asp Ile                  10 15 20 gca aaa tct gca cac ttc tac gaa aac gtt ctc ggc tgg gaa att gaa 211 Ala Lys Ser Ala His Phe Tyr Glu Asn Val Leu Gly Trp Glu Ile Glu              25 30 35 gaa gtc aac gat ggc tac cgc atg gct cgt ctg cag gga cta ccc gtg 259 Glu Val Asn Asp Gly Tyr Arg Met Ala Arg Leu Gln Gly Leu Pro Val          40 45 50 gca ggg ctg atc gat cag cgc ggt gaa tca agc atc ccg gat acc tgg 307 Ala Gly Leu Ile Asp Gln Arg Gly Glu Ser Ser Ile Pro Asp Thr Trp      55 60 65 att acc tac ttc ctc tcc tac gat ctg gat gcc act gca aag aag atc 355 Ile Thr Tyr Phe Leu Ser Tyr Asp Leu Asp Ala Thr Ala Lys Lys Ile  70 75 80 85 gca gaa ctg ggt gga cga att ctg gcc gag cca act gac gtg cac ttg 403 Ala Glu Leu Gly Gly Arg Ile Leu Ala Glu Pro Thr Asp Val His Leu                  90 95 100 gga cgc atg atc cta gct gtt gat act gcc ggc gca ctg ttc ggc gtt 451 Gly Arg Met Ile Leu Ala Val Asp Thr Ala Gly Ala Leu Phe Gly Val             105 110 115 att gag cca ggc agc gag gaa tca ttc gtc gct gct ggt gaa cca ggc 499 Ile Glu Pro Gly Ser Glu Glu Ser Phe Val Ala Ala Gly Glu Pro Gly         120 125 130 aca tcc gtg tgg cat gaa ctc acc act gtc tcc aaa tat tcc gaa gct 547 Thr Ser Val Trp His Glu Leu Thr Thr Val Ser Lys Tyr Ser Glu Ala     135 140 145 atc gat ttc tac ggt gag ctg ttc act tgg aca acc tct gaa atg gct 595 Ile Asp Phe Tyr Gly Glu Leu Phe Thr Trp Thr Thr Ser Glu Met Ala 150 155 160 165 agt gct gaa gac gat agt ttc cgc tac acc acc gca ttg gct gac ggt 643 Ser Ala Glu Asp Asp Ser Phe Arg Tyr Thr Thr Ala Leu Ala Asp Gly                 170 175 180 tcc gcc ttt gct gga att ttt gat gcc aaa ggc cac ttc cca cct cag 691 Ser Ala Phe Ala Gly Ile Phe Asp Ala Lys Gly His Phe Pro Pro Gln             185 190 195 gtt cca agc ttc tgg cag tcc tac ctt ggc gtg ctc aac gcc gat gat 739 Val Pro Ser Phe Trp Gln Ser Tyr Leu Gly Val Leu Asn Ala Asp Asp         200 205 210 gct gca gcg aag gcc aag gaa ttt ggt ggc gat gtt att cgt aag cca 787 Ala Ala Ala Lys Ala Lys Glu Phe Gly Gly Asp Val Ile Arg Lys Pro     215 220 225 tgg gac tca gaa ttt ggc cgc atg gtt ctc atc tct gat tcc act ggt 835 Trp Asp Ser Glu Phe Gly Arg Met Val Leu Ile Ser Asp Ser Thr Gly 230 235 240 245 gcc aca att acc ttg tgt gaa gta gag gaa tac gtc gag gaa gca gca 883 Ala Thr Ile Thr Leu Cys Glu Val Glu Glu Tyr Val Glu Glu Ala Ala                 250 255 260 gaa ggc gat gat ctc ttc gac atc gat ctc agt gct ttc gaa gag cag 931 Glu Gly Asp Asp Leu Phe Asp Ile Asp Leu Ser Ala Phe Glu Glu Gln             265 270 275 ttc cgc aag caa gaa gga cag taatcctaca gcgccatgga gga 975 Phe Arg Lys Gln Glu Gly Gln         280 <210> 188 <211> 284 <212> PRT <213> Corynebacterium glutamicum <400> 188 Met Pro Ala Phe Glu Ala Met Pro Gly Met Pro Tyr Trp Ile Asp Leu   1 5 10 15 Ser Thr Ser Asp Ile Ala Lys Ser Ala His Phe Tyr Glu Asn Val Leu              20 25 30 Gly Trp Glu Ile Glu Glu Val Asn Asp Gly Tyr Arg Met Ala Arg Leu          35 40 45 Gln Gly Leu Pro Val Ala Gly Leu Ile Asp Gln Arg Gly Glu Ser Ser      50 55 60 Ile Pro Asp Thr Trp Ile Thr Tyr Phe Leu Ser Tyr Asp Leu Asp Ala  65 70 75 80 Thr Ala Lys Lys Ile Ala Glu Leu Gly Gly Arg Ile Leu Ala Glu Pro                  85 90 95 Thr Asp Val His Leu Gly Arg Met Ile Leu Ala Val Asp Thr Ala Gly             100 105 110 Ala Leu Phe Gly Val Ile Glu Pro Gly Ser Glu Glu Ser Phe Val Ala         115 120 125 Ala Gly Glu Pro Gly Thr Ser Val Trp His Glu Leu Thr Thr Val Ser     130 135 140 Lys Tyr Ser Glu Ala Ile Asp Phe Tyr Gly Glu Leu Phe Thr Trp Thr 145 150 155 160 Thr Ser Glu Met Ala Ser Ala Glu Asp Asp Ser Phe Arg Tyr Thr Thr                 165 170 175 Ala Leu Ala Asp Gly Ser Ala Phe Ala Gly Ile Phe Asp Ala Lys Gly             180 185 190 His Phe Pro Pro Gln Val Pro Ser Phe Trp Gln Ser Tyr Leu Gly Val         195 200 205 Leu Asn Ala Asp Asp Ala Ala Ala Lys Ala Lys Glu Phe Gly Gly Asp     210 215 220 Val Ile Arg Lys Pro Trp Asp Ser Glu Phe Gly Arg Met Val Leu Ile 225 230 235 240 Ser Asp Ser Thr Gly Ala Thr Ile Thr Leu Cys Glu Val Glu Glu Tyr                 245 250 255 Val Glu Glu Ala Ala Glu Gly Asp Asp Leu Phe Asp Ile Asp Leu Ser             260 265 270 Ala Phe Glu Glu Gln Phe Arg Lys Gln Glu Gly Gln         275 280 <210> 189 <211> 948 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101) .. (925) <223> RXA00084 <400> 189 tcacccttgt cgataccagc tactgggtat ctggcgtcgg tccacttggc ggcagcaaag 60 tcttggaaga catcgatgcc ttcctcgacg cacagcaata atg tcc aca gct ctc 115                                             Met ser thr ala leu                                               1 5 ccc gat cag ctc aag tgg gaa tac agt gcc ttc ccc gtg cag atc tcg 163 Pro Asp Gln Leu Lys Trp Glu Tyr Ser Ala Phe Pro Val Gln Ile Ser                  10 15 20 cag aag caa cgg ctt agt ccc ggc ttc atg cgg atc acc gtc act ggt 211 Gln Lys Gln Arg Leu Ser Pro Gly Phe Met Arg Ile Thr Val Thr Gly              25 30 35 gac aag ctc cga ttc ttt ggc cag tgg ggt ttg gac caa cgc atc aaa 259 Asp Lys Leu Arg Phe Phe Gly Gln Trp Gly Leu Asp Gln Arg Ile Lys          40 45 50 ctg atc att cca agc ccg gct ggg aac atc cca gat ttc gga att ctc 307 Leu Ile Ile Pro Ser Pro Ala Gly Asn Ile Pro Asp Phe Gly Ile Leu      55 60 65 gac gaa ccc act ccc cca ccg aca acg tgg ctt cct cgt gct aag tct 355 Asp Glu Pro Thr Pro Pro Pro Thr Thr Trp Leu Pro Arg Ala Lys Ser  70 75 80 85 ttt cca gcg gac caa cga ccg atc ttg cgc acc tac acc cca tct gcg 403 Phe Pro Ala Asp Gln Arg Pro Ile Leu Arg Thr Tyr Thr Pro Ser Ala                  90 95 100 gtc cga ccc gaa cta tgc gaa gta gac att gat atc tat ctt cac aac 451 Val Arg Pro Glu Leu Cys Glu Val Asp Ile Asp Ile Tyr Leu His Asn             105 110 115 cct tcg gga cca gta tcc aga tgg gca aag aac tgc agt gtt gac gat 499 Pro Ser Gly Pro Val Ser Arg Trp Ala Lys Asn Cys Ser Val Asp Asp         120 125 130 gaa cta atc atc acc ggc cct gac gta cgc gca gga gaa acc ggc tac 547 Glu Leu Ile Ile Thr Gly Pro Asp Val Arg Ala Gly Glu Thr Gly Tyr     135 140 145 gga atc acc tat cat ccg act tct gcg atc gat cgc ctc tgt ctc atc 595 Gly Ile Thr Tyr His Pro Thr Ser Ala Ile Asp Arg Leu Cys Leu Ile 150 155 160 165 ggc gat tgt gca tca gct ccc gcg atc gca aat atc gtc aat caa tca 643 Gly Asp Cys Ala Ser Ala Pro Ala Ile Ala Asn Ile Val Asn Gln Ser                 170 175 180 aaa gta cct act acg gtt ttc ctc cac gta gac agc cta gaa gat gat 691 Lys Val Pro Thr Thr Val Phe Leu His Val Asp Ser Leu Glu Asp Asp             185 190 195 gta ttg atc gcc gat agc tcc acc aag ctc act ttc gaa gac atc gac 739 Val Leu Ile Ala Asp Ser Ser Thr Lys Leu Thr Phe Glu Asp Ile Asp         200 205 210 gct tac aaa gca aag gtc ttc caa tgg gct tca gcc aat gca gca gat 787 Ala Tyr Lys Ala Lys Val Phe Gln Trp Ala Ser Ala Asn Ala Ala Asp     215 220 225 cct tca gta cac ttc tgg atc gcc ggt gaa act agc atg gtg cgc ttc 835 Pro Ser Val His Phe Trp Ile Ala Gly Glu Thr Ser Met Val Arg Phe 230 235 240 245 att cgc aaa gaa cta atc aac agc tac cga gtt gat tcc tca cga atc 883 Ile Arg Lys Glu Leu Ile Asn Ser Tyr Arg Val Asp Ser Ser Arg Ile                 250 255 260 act ttc ctc ggc tac tgg aaa tac ggc cga cga acc gta gac 925 Thr Phe Leu Gly Tyr Trp Lys Tyr Gly Arg Arg Thr Val Asp             265 270 275 tagctttcag attcagaccc cag 948 <210> 190 <211> 275 <212> PRT <213> Corynebacterium glutamicum <400> 190 Met Ser Thr Ala Leu Pro Asp Gln Leu Lys Trp Glu Tyr Ser Ala Phe   1 5 10 15 Pro Val Gln Ile Ser Gln Lys Gln Arg Leu Ser Pro Gly Phe Met Arg              20 25 30 Ile Thr Val Thr Gly Asp Lys Leu Arg Phe Phe Gly Gln Trp Gly Leu          35 40 45 Asp Gln Arg Ile Lys Leu Ile Ile Pro Ser Pro Ala Gly Asn Ile Pro      50 55 60 Asp Phe Gly Ile Leu Asp Glu Pro Thr Pro Pro Pro Thr Thr Trp Leu  65 70 75 80 Pro Arg Ala Lys Ser Phe Pro Ala Asp Gln Arg Pro Ile Leu Arg Thr                  85 90 95 Tyr Thr Pro Ser Ala Val Arg Pro Glu Leu Cys Glu Val Asp Ile Asp             100 105 110 Ile Tyr Leu His Asn Pro Ser Gly Pro Val Ser Arg Trp Ala Lys Asn         115 120 125 Cys Ser Val Asp Asp Glu Leu Ile Ill Thr Gly Pro Asp Val Arg Ala     130 135 140 Gly Glu Thr Gly Tyr Gly Ile Thr Tyr His Pro Thr Ser Ala Ile Asp 145 150 155 160 Arg Leu Cys Leu Ile Gly Asp Cys Ala Ser Ala Pro Ala Ile Ala Asn                 165 170 175 Ile Val Asn Gln Ser Lys Val Pro Thr Thr Val Phe Leu His Val Asp             180 185 190 Ser Leu Glu Asp Asp Val Leu Ile Ala Asp Ser Ser Thr Lys Leu Thr         195 200 205 Phe Glu Asp Ile Asp Ala Tyr Lys Ala Lys Val Phe Gln Trp Ala Ser     210 215 220 Ala Asn Ala Ala Asp Pro Ser Val His Phe Trp Ile Ala Gly Glu Thr 225 230 235 240 Ser Met Val Arg Phe Ile Arg Lys Glu Leu Ile Asn Ser Tyr Arg Val                 245 250 255 Asp Ser Ser Arg Ile Thr Phe Leu Gly Tyr Trp Lys Tyr Gly Arg Arg             260 265 270 Thr val asp         275 <210> 191 <211> 468 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (445) <223> RXA00843 <400> 191 gccctgatgc gaaaccggcg ccaacaatga tgccgacgaa ggcaaatgcc actcttagga 60 tttgaataat catggaacaa accttagtag gctcaacgtt atg aaa gtc acg att 115                                             Met Lys Val Thr Ile                                               1 5 ttc cat aat ccg cgt tgt tcc aca tcc aga aat acc ctc gct tac ctc 163 Phe His Asn Pro Arg Cys Ser Thr Ser Arg Asn Thr Leu Ala Tyr Leu                  10 15 20 cgc gac aag gac att gag cct gaa att gtt cag tat ctc aaa gac acg 211 Arg Asp Lys Asp Ile Glu Pro Glu Ile Val Gln Tyr Leu Lys Asp Thr              25 30 35 ccc acc gct tcc gag ctc aaa gaa cta ttc aat acg ctg gga att cca 259 Pro Thr Ala Ser Glu Leu Lys Glu Leu Phe Asn Thr Leu Gly Ile Pro          40 45 50 gtc cac gac ggc atc aga acc cgc gaa gct gag tac aca gaa ctg ggc 307 Val His Asp Gly Ile Arg Thr Arg Glu Ala Glu Tyr Thr Glu Leu Gly      55 60 65 ctg tca cca gaa aca cct gaa act gag ctt atc gac gcc atc gtt gcc 355 Leu Ser Pro Glu Thr Pro Glu Thr Glu Leu Ile Asp Ala Ile Val Ala  70 75 80 85 cat ccc agg ctc ctt cag cgt ccg atc gtg gtg acg gcc aaa ggc gcg 403 His Pro Arg Leu Leu Gln Arg Pro Ile Val Val Thr Ala Lys Gly Ala                  90 95 100 cgc att gcg cgc ccc aaa atc gac gtc att gac agc atc ttg 445 Arg Ile Ala Arg Pro Lys Ile Asp Val Ile Asp Ser Ile Leu             105 110 115 tgacaacatt ttgtagagca acc 468 <210> 192 <211> 115 <212> PRT <213> Corynebacterium glutamicum <400> 192 Met Lys Val Thr Ile Phe His Asn Pro Arg Cys Ser Thr Ser Arg Asn   1 5 10 15 Thr Leu Ala Tyr Leu Arg Asp Lys Asp Ile Glu Pro Glu Ile Val Gln              20 25 30 Tyr Leu Lys Asp Thr Pro Thr Ala Ser Glu Leu Lys Glu Leu Phe Asn          35 40 45 Thr Leu Gly Ile Pro Val His Asp Gly Ile Arg Thr Arg Glu Ala Glu      50 55 60 Tyr Thr Glu Leu Gly Leu Ser Pro Glu Thr Pro Glu Thr Glu Leu Ile  65 70 75 80 Asp Ala Ile Val Ala His Pro Arg Leu Leu Gln Arg Pro Ile Val Val                  85 90 95 Thr Ala Lys Gly Ala Arg Ile Ala Arg Pro Lys Ile Asp Val Ile Asp             100 105 110 Ser ile leu         115 <210> 193 <211> 432 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (409) <223> RXA01052 <400> 193 tatggccaac cctaggggga tggcctgtgt gttcactgtt aggtttcctc aaaatcttta 60 acgaacaacg aagagcttgc ccgagagtat cttgggtcgc atg gac aca aaa tta 115                                             Met Asp Thr Lys Leu                                               1 5 ggc gct gaa ttg ggt act gaa ttt gat ctc att gtt gtt ggt ttc ggc 163 Gly Ala Glu Leu Gly Thr Glu Phe Asp Leu Ile Val Val Gly Phe Gly                  10 15 20 aaa gca ggc aag act atc gcg atg aaa cgc tcg gca gcg ggg gat aag 211 Lys Ala Gly Lys Thr Ile Ala Met Lys Arg Ser Ala Ala Gly Asp Lys              25 30 35 gtc gca ctg atc gag cag agt cca cag atg tat ggc ggt acc tgc atc 259 Val Ala Leu Ile Glu Gln Ser Pro Gln Met Tyr Gly Gly Thr Cys Ile          40 45 50 aat gta ggt tgc atc ccc acg aag aag ttg ttg ttt gag act gca acg 307 Asn Val Gly Cys Ile Pro Thr Lys Lys Leu Leu Phe Glu Thr Ala Thr      55 60 65 ggc aag gat ttc ccg gat gcg gtt gtg gcg cgt gat cag ttg att ggc 355 Gly Lys Asp Phe Pro Asp Ala Val Val Ala Arg Asp Gln Leu Ile Gly  70 75 80 85 aag ctg aat gcc aag aat ctt gcg atg gcc aca gac aag ggt gtc acc 403 Lys Leu Asn Ala Lys Asn Leu Ala Met Ala Thr Asp Lys Gly Val Thr                  90 95 100 cgt cat tgatggaaaa gctacgttta cag 432 Arg his          <210> 194 <211> 103 <212> PRT <213> Corynebacterium glutamicum <400> 194 Met Asp Thr Lys Leu Gly Ala Glu Leu Gly Thr Glu Phe Asp Leu Ile   1 5 10 15 Val Val Gly Phe Gly Lys Ala Gly Lys Thr Ile Ala Met Lys Arg Ser              20 25 30 Ala Ala Gly Asp Lys Val Ala Leu Ile Glu Gln Ser Pro Gln Met Tyr          35 40 45 Gly Gly Thr Cys Ile Asn Val Gly Cys Ile Pro Thr Lys Lys Leu Leu      50 55 60 Phe Glu Thr Ala Thr Gly Lys Asp Phe Pro Asp Ala Val Val Ala Arg  65 70 75 80 Asp Gln Leu Ile Gly Lys Leu Asn Ala Lys Asn Leu Ala Met Ala Thr                  85 90 95 Asp Lys Gly Val Thr Arg His             100 <210> 195 <211> 543 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101) .. (520) <223> RXA01053 <400> 195 ttgcgatggc cacagacaag ggtgtcaccc gtcattgatg gaaaagctac gtttacagct 60 agccacgaaa tcacaagtaa cttcaggtag tgacactctt gtg ctg tat gcg cca 115                                             Val Leu Tyr Ala Pro                                               1 5 acg att gtg atc aac acg ggc tcc acg ccg gtc atc ccc aat gtc cca 163 Thr Ile Val Ile Asn Thr Gly Ser Thr Pro Val Ile Pro Asn Val Pro                  10 15 20 ggc acc gac aat ccg cat gtt ttt gat tcc act ggc att cag cac att 211 Gly Thr Asp Asn Pro His Val Phe Asp Ser Thr Gly Ile Gln His Ile              25 30 35 tcg ccc ctg ccg aag cac ctc gcg atc atc ggc ggt ggc ccc atc ggt 259 Ser Pro Leu Pro Lys His Leu Ala Ile Ile Gly Gly Gly Pro Ile Gly          40 45 50 ttg gaa ttt gcc acg ctt ttc agt gga caa ggc tcc aaa gtc acc atc 307 Leu Glu Phe Ala Thr Leu Phe Ser Gly Gln Gly Ser Lys Val Thr Ile      55 60 65 atc gac cgt ggt gaa ttg ccg ctg aaa aat ttc gac agg gaa gta gcg 355 Ile Asp Arg Gly Glu Leu Pro Leu Lys Asn Phe Asp Arg Glu Val Ala  70 75 80 85 gag ctg gcc aaa acc gac ctg gag gcc cgc gga atc acc ttc ctc aac 403 Glu Leu Ala Lys Thr Asp Leu Glu Ala Arg Gly Ile Thr Phe Leu Asn                  90 95 100 aac gct gaa ctc acc gga ttc agc ggt gac ctc acc atc gcg ctc aaa 451 Asn Ala Glu Leu Thr Gly Phe Ser Gly Asp Leu Thr Ile Ala Leu Lys             105 110 115 gac cac gac ctc ctc gcc gac gcc gca ctt ttt gca tcg gcc gac gcc 499 Asp His Asp Leu Leu Ala Asp Ala Ala Leu Phe Ala Ser Ala Asp Ala         120 125 130 cgg cac cga cgg gct cgg cct tgaacaggcg ggcatcaaaa cag 543 Arg His Arg Arg Ala Arg Pro     135 140 <210> 196 <211> 140 <212> PRT <213> Corynebacterium glutamicum <400> 196 Val Leu Tyr Ala Pro Thr Ile Val Ile Asn Thr Gly Ser Thr Pro Val   1 5 10 15 Ile Pro Asn Val Pro Gly Thr Asp Asn Pro His Val Phe Asp Ser Thr              20 25 30 Gly Ile Gln His Ile Ser Pro Leu Pro Lys His Leu Ala Ile Ile Gly          35 40 45 Gly Gly Pro Ile Gly Leu Glu Phe Ala Thr Leu Phe Ser Gly Gln Gly      50 55 60 Ser Lys Val Thr Ile Ile Asp Arg Gly Glu Leu Pro Leu Lys Asn Phe  65 70 75 80 Asp Arg Glu Val Ala Glu Leu Ala Lys Thr Asp Leu Glu Ala Arg Gly                  85 90 95 Ile Thr Phe Leu Asn Asn Ala Glu Leu Thr Gly Phe Ser Gly Asp Leu             100 105 110 Thr Ile Ala Leu Lys Asp His Asp Leu Leu Ala Asp Ala Ala Leu Phe         115 120 125 Ala Ser Ala Asp Ala Arg His Arg Arg Ala Arg Pro     130 135 140 <210> 197 <211> 612 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (589) <223> RXA01054 <400> 197 gacctcctcg ccgacgccgc actttttgca tcggccgacg cccggcaccg acgggctcgg 60 ccttgaacag gcgggcatca aaacaggcac gcgtggggag gtg ctt gtc gac gcc 115                                             Val Leu Val Asp Ala                                               1 5 cac ctc cgg acc aac atc gac ggc atc ttc gct gta ggt gat gtc aat 163 His Leu Arg Thr Asn Ile Asp Gly Ile Phe Ala Val Gly Asp Val Asn                  10 15 20 ggc ggc ccg cag ttt acc tac gtg tcc tac gat gac cac cgc att gtg 211 Gly Gly Pro Gln Phe Thr Tyr Val Ser Tyr Asp Asp His Arg Ile Val              25 30 35 ctg gat caa cta gcc gga aca ggt aag aaa tcc att gca cac cga ctg 259 Leu Asp Gln Leu Ala Gly Thr Gly Lys Lys Ser Ile Ala His Arg Leu          40 45 50 atc ccc acc acc acg ttc atc gaa ccg ccg tta tcc acc atc ggt gac 307 Ile Pro Thr Thr Thr Phe Ile Glu Pro Pro Leu Ser Thr Ile Gly Asp      55 60 65 aac act gaa ggg gaa aat gtg gtg gtg aaa aag gcc ttg att gca gat 355 Asn Thr Glu Gly Glu Asn Val Val Val Lys Lys Ala Leu Ile Ala Asp  70 75 80 85 atg ccg atc gtt ccc cga cca gag att att aac caa cct cac ggt atg 403 Met Pro Ile Val Pro Arg Pro Glu Ile Ile Asn Gln Pro His Gly Met                  90 95 100 gtg aag ttt ttc gtc gac aag caa tct gat gcg ctg ctc ggc gcg acc 451 Val Lys Phe Phe Val Asp Lys Gln Ser Asp Ala Leu Leu Gly Ala Thr             105 110 115 ttg tac tgc gcc gac tcc cag gag ctc atc aac acc gtg gcg ctt gcc 499 Leu Tyr Cys Ala Asp Ser Gln Glu Leu Ile Asn Thr Val Ala Leu Ala         120 125 130 atg cgg cat ggc gtc acc gcc tcc gag ctt ggc gac ggc atc tac acc 547 Met Arg His Gly Val Thr Ala Ser Glu Leu Gly Asp Gly Ile Tyr Thr     135 140 145 cac ccc gcc acc tcg gag atc ttc aac caa tta ttg ggc agt 589 His Pro Ala Thr Ser Glu Ile Phe Asn Gln Leu Leu Gly Ser 150 155 160 taacgcagcg gatcgaacgg ctt 612 <210> 198 <211> 163 <212> PRT <213> Corynebacterium glutamicum <400> 198 Val Leu Val Asp Ala His Leu Arg Thr Asn Ile Asp Gly Ile Phe Ala   1 5 10 15 Val Gly Asp Val Asn Gly Gly Pro Gln Phe Thr Tyr Val Ser Tyr Asp              20 25 30 Asp His Arg Ile Val Leu Asp Gln Leu Ala Gly Thr Gly Lys Lys Ser          35 40 45 Ile Ala His Arg Leu Ile Pro Thr Thr Thr Phe Ile Glu Pro Pro Leu      50 55 60 Ser Thr Ile Gly Asp Asn Thr Glu Gly Glu Asn Val Val Val Lys Lys  65 70 75 80 Ala Leu Ile Ala Asp Met Pro Ile Val Pro Arg Pro Glu Ile Ile Asn                  85 90 95 Gln Pro His Gly Met Val Lys Phe Phe Val Asp Lys Gln Ser Asp Ala             100 105 110 Leu Leu Gly Ala Thr Leu Tyr Cys Ala Asp Ser Gln Glu Leu Ile Asn         115 120 125 Thr Val Ala Leu Ala Met Arg His Gly Val Thr Ala Ser Glu Leu Gly     130 135 140 Asp Gly Ile Tyr Thr His Pro Ala Thr Ser Glu Ile Phe Asn Gln Leu 145 150 155 160 Leu gly ser              <210> 199 <211> 561 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (538) <223> RXN03123 <400> 199 agctctacca acgcgcctac accttgacca acgtggatgc cgatgccggt acctttgacc 60 tggcttttgt gctgcacgag ccgctggggc ccgcctcggc gtg ggc gac gcg ctg 115                                             Val Gly Asp Ala Leu                                               1 5 cga ggc cgg gga aag cct gaa gtc atg cgc tac cca gga att ccg ttc 163 Arg Gly Arg Gly Lys Pro Glu Val Met Arg Tyr Pro Gly Ile Pro Phe                  10 15 20 gcc atc cca gat cca gcg ccg cgt ggc ttc ctt ttc tta ggc gat ctc 211 Ala Ile Pro Asp Pro Ala Pro Arg Gly Phe Leu Phe Leu Gly Asp Leu              25 30 35 acc tct tac cca gcg atc tgc tcg att ctg gag acc ttg gac ggt gaa 259 Thr Ser Tyr Pro Ala Ile Cys Ser Ile Leu Glu Thr Leu Asp Gly Glu          40 45 50 atc cct gcg acc gcg tat ctt atc gcc cac gat cca ctt gat tac acc 307 Ile Pro Ala Thr Ala Tyr Leu Ile Ala His Asp Pro Leu Asp Tyr Thr      55 60 65 ttc gat ttt ccc cag ggc gag cac atc acc gcg cag tgg att tcc aac 355 Phe Asp Phe Pro Gln Gly Glu His Ile Thr Ala Gln Trp Ile Ser Asn  70 75 80 85 gaa caa tcc ttc att gat cac atc gct gac acg gat tac acc gat ttt 403 Glu Gln Ser Phe Ile Asp His Ile Ala Asp Thr Asp Tyr Thr Asp Phe                  90 95 100 tat acc tgg atc ggc gcg gaa tcc tcc gaa acc cgt gcg gcc aag aag 451 Tyr Thr Trp Ile Gly Ala Glu Ser Ser Glu Thr Arg Ala Ala Lys Lys             105 110 115 cat ctg cag acc cac gcc ggc atg ccc aag acg cac atg aac gcg caa 499 His Leu Gln Thr His Ala Gly Met Pro Lys Thr His Met Asn Ala Gln         120 125 130 ggt tat tgg aac aag ggc aga gcc atg ggt aaa agc aat taaaagattt 548 Gly Tyr Trp Asn Lys Gly Arg Ala Met Gly Lys Ser Asn     135 140 145 ttgcttatcg acg 561 <210> 200 <211> 146 <212> PRT <213> Corynebacterium glutamicum <400> 200 Val Gly Asp Ala Leu Arg Gly Arg Gly Lys Pro Glu Val Met Arg Tyr   1 5 10 15 Pro Gly Ile Pro Phe Ala Ile Pro Asp Pro Ala Pro Arg Gly Phe Leu              20 25 30 Phe Leu Gly Asp Leu Thr Ser Tyr Pro Ala Ile Cys Ser Ile Leu Glu          35 40 45 Thr Leu Asp Gly Glu Ile Pro Ala Thr Ala Tyr Leu Ile Ala His Asp      50 55 60 Pro Leu Asp Tyr Thr Phe Asp Phe Pro Gln Gly Glu His Ile Thr Ala  65 70 75 80 Gln Trp Ile Ser Asn Glu Gln Ser Phe Ile Asp His Ile Ala Asp Thr                  85 90 95 Asp Tyr Thr Asp Phe Tyr Thr Trp Ile Gly Ala Glu Ser Ser Glu Thr             100 105 110 Arg Ala Ala Lys Lys His Leu Gln Thr His Ala Gly Met Pro Lys Thr         115 120 125 His Met Asn Ala Gln Gly Tyr Trp Asn Lys Gly Arg Ala Met Gly Lys     130 135 140 Ser asn 145 <210> 201 <211> 736 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (736) <223> FRXA00993 <400> 201 gctgagctag tgcttttgcg acacacctct tgcgaatgtt gattaggtta ggcaagccat 60 atttacaggg tgttgtgaaa gcataaggga gcaaggaaac atg ggc aag gga ttt 115                                             Met Gly Lys Gly Phe                                               1 5 acc ggc gct att ttg acc gtc atg ggc gtg aaa tcg cat atc gcc acc 163 Thr Gly Ala Ile Leu Thr Val Met Gly Val Lys Ser His Ile Ala Thr                  10 15 20 acc acg gga aaa acc gtg atc aat gac cgc atg gtg acc att cat ttt 211 Thr Thr Gly Lys Thr Val Ile Asn Asp Arg Met Val Thr Ile His Phe              25 30 35 cat tcc gag acg ctg ctc aac acg gaa ggt gaa gtc ccc ggc gat tgg 259 His Ser Glu Thr Leu Leu Asn Thr Glu Gly Glu Val Pro Gly Asp Trp          40 45 50 ctg cgt ctg tgg ttc ccg cac gag agc cga cct gga aag ctc tac caa 307 Leu Arg Leu Trp Phe Pro His Glu Ser Arg Pro Gly Lys Leu Tyr Gln      55 60 65 cgc gcc tac acc ttg acc aac gtg gat gcc gat gcc ggt acc ttt gac 355 Arg Ala Tyr Thr Leu Thr Asn Val Asp Ala Asp Ala Gly Thr Phe Asp  70 75 80 85 ctg gct ttt gtg ctg cac gag ccg ctg ggg ccc gcc tcg gcg tgg gcg 403 Leu Ala Phe Val Leu His Glu Pro Leu Gly Pro Ala Ser Ala Trp Ala                  90 95 100 acg cgc tgc gag gcc ggg gaa agc ctg gaa gtc atg cgc tac cca gga 451 Thr Arg Cys Glu Ala Gly Glu Ser Leu Glu Val Met Arg Tyr Pro Gly             105 110 115 att ccg ttc gcc atc cca gat cca gcg ccg cgt ggc ttc ctt ttc cta 499 Ile Pro Phe Ala Ile Pro Asp Pro Ala Pro Arg Gly Phe Leu Phe Leu         120 125 130 ggc gat ctc acc tct tac cca gcg atc tgc tcg att ctg gag acc ttg 547 Gly Asp Leu Thr Ser Tyr Pro Ala Ile Cys Ser Ile Leu Glu Thr Leu     135 140 145 gac ggt gaa atc cct gcg acc gcg tat ctt atc gcc cac gat cca ctt 595 Asp Gly Glu Ile Pro Ala Thr Ala Tyr Leu Ile Ala His Asp Pro Leu 150 155 160 165 gat tac acc ttc gat ttt ccc cag ggc gag cac atc acc gcg cag tgg 643 Asp Tyr Thr Phe Asp Phe Pro Gln Gly Glu His Ile Thr Ala Gln Trp                 170 175 180 att tcc aac gaa caa tcc ttc att gat cac atc gct gac acg gat tac 691 Ile Ser Asn Glu Gln Ser Phe Ile Asp His Ile Ala Asp Thr Asp Tyr             185 190 195 acc gat ttt tat acc tgg atc ggc gcg gaa tcc tcc gaa acc cgt 736 Thr Asp Phe Tyr Thr Trp Ile Gly Ala Glu Ser Ser Glu Thr Arg         200 205 210 <210> 202 <211> 212 <212> PRT <213> Corynebacterium glutamicum <400> 202 Met Gly Lys Gly Phe Thr Gly Ala Ile Leu Thr Val Met Gly Val Lys   1 5 10 15 Ser His Ile Ala Thr Thr Thr Gly Lys Thr Val Ile Asn Asp Arg Met              20 25 30 Val Thr Ile His Phe His Ser Glu Thr Leu Leu Asn Thr Glu Gly Glu          35 40 45 Val Pro Gly Asp Trp Leu Arg Leu Trp Phe Pro His Glu Ser Arg Pro      50 55 60 Gly Lys Leu Tyr Gln Arg Ala Tyr Thr Leu Thr Asn Val Asp Ala Asp  65 70 75 80 Ala Gly Thr Phe Asp Leu Ala Phe Val Leu His Glu Pro Leu Gly Pro                  85 90 95 Ala Ser Ala Trp Ala Thr Arg Cys Glu Ala Gly Glu Ser Leu Glu Val             100 105 110 Met Arg Tyr Pro Gly Ile Pro Phe Ala Ile Pro Asp Pro Ala Pro Arg         115 120 125 Gly Phe Leu Phe Leu Gly Asp Leu Thr Ser Tyr Pro Ala Ile Cys Ser     130 135 140 Ile Leu Glu Thr Leu Asp Gly Glu Ile Pro Ala Thr Ala Tyr Leu Ile 145 150 155 160 Ala His Asp Pro Leu Asp Tyr Thr Phe Asp Phe Pro Gln Gly Glu His                 165 170 175 Ile Thr Ala Gln Trp Ile Ser Asn Glu Gln Ser Phe Ile Asp His Ile             180 185 190 Ala Asp Thr Asp Tyr Thr Asp Phe Tyr Thr Trp Ile Gly Ala Glu Ser         195 200 205 Ser Glu Thr Arg     210 <210> 203 <211> 732 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (709) <223> RXA01051 <400> 203 tgcgacccaa gatactctcg ggcaagctct tcgttgttcg ttaaagattt tgaggaaacc 60 taacagtgaa cacacaggcc atccccctag ggttggccat atg tca acc att cac 115                                             Met Ser Thr Ile His                                               1 5 gcc tcc gga atc cag gct cca caa gtg cca cac ggt tcc cac cat gcc 163 Ala Ser Gly Ile Gln Ala Pro Gln Val Pro His Gly Ser His His Ala                  10 15 20 ccg cca caa aag gac gaa tca gtg aag aag agc ttc aat gcc tct tct 211 Pro Pro Gln Lys Asp Glu Ser Val Lys Lys Ser Phe Asn Ala Ser Ser              25 30 35 tta ctg ttc gcg ttt tcc ttc ggc gtg tac ctg gtg ctg ctt gtg atg 259 Leu Leu Phe Ala Phe Ser Phe Gly Val Tyr Leu Val Leu Leu Val Met          40 45 50 atg aca ctt ctt aaa agt cgc ctt tct tta ggc gga ctg tgg aac aca 307 Met Thr Leu Leu Lys Ser Arg Leu Ser Leu Gly Gly Leu Trp Asn Thr      55 60 65 gaa gca cac caa tac aga tcc atc gac tta gag ctt ttc aac ggc ttt 355 Glu Ala His Gln Tyr Arg Ser Ile Asp Leu Glu Leu Phe Asn Gly Phe  70 75 80 85 gct gat cca cca att tgg tgg ggg cct tgg acc aac act ttt ggc aac 403 Ala Asp Pro Pro Ile Trp Trp Gly Pro Trp Thr Asn Thr Phe Gly Asn                  90 95 100 atc gca ctg ttc atg cca ttt ggg ttt ttc ctg tac aaa atg ctc cgt 451 Ile Ala Leu Phe Met Pro Phe Gly Phe Phe Leu Tyr Lys Met Leu Arg             105 110 115 aga ttc aac cat cga ttc ccc ttc gta gaa acc atc ctg ttt gcc agc 499 Arg Phe Asn His Arg Phe Pro Phe Val Glu Thr Ile Leu Phe Ala Ser         120 125 130 gtc acc agc ctc agt atc gaa gtt ctg caa tgg gtg ttt gct att gga 547 Val Thr Ser Leu Ser Ile Glu Val Leu Gln Trp Val Phe Ala Ile Gly     135 140 145 tat tca gat gtc gat gac ctg ttg ttt aat acg atc ggc gga ctc att 595 Tyr Ser Asp Val Asp Asp Leu Leu Phe Asn Thr Ile Gly Gly Leu Ile 150 155 160 165 gga gca tcc gta gca gcg ctt gtc tcg ctt aaa tcc tcc aag gta gtc 643 Gly Ala Ser Val Ala Ala Leu Val Ser Leu Lys Ser Ser Lys Val Val                 170 175 180 agc gga atc atc atg ggc ggt tca cta tct gtg atg gcg atg atg atg 691 Ser Gly Ile Ile Met Gly Gly Ser Leu Ser Val Met Ala Met Met Met             185 190 195 tat tca agt ttt atc gcc tagaaggttt cagcagttcc gct 732 Tyr Ser Ser Phe Ile Ala         200 <210> 204 <211> 203 <212> PRT <213> Corynebacterium glutamicum <400> 204 Met Ser Thr Ile His Ala Ser Gly Ile Gln Ala Pro Gln Val Pro His   1 5 10 15 Gly Ser His His Ala Pro Pro Gln Lys Asp Glu Ser Val Lys Lys Ser              20 25 30 Phe Asn Ala Ser Ser Leu Leu Phe Ala Phe Ser Phe Gly Val Tyr Leu          35 40 45 Val Leu Leu Val Met Met Thr Leu Leu Lys Ser Arg Leu Ser Leu Gly      50 55 60 Gly Leu Trp Asn Thr Glu Ala His Gln Tyr Arg Ser Ile Asp Leu Glu  65 70 75 80 Leu Phe Asn Gly Phe Ala Asp Pro Pro Ile Trp Trp Gly Pro Trp Thr                  85 90 95 Asn Thr Phe Gly Asn Ile Ala Leu Phe Met Pro Phe Gly Phe Phe Leu             100 105 110 Tyr Lys Met Leu Arg Arg Phe Asn His Arg Phe Pro Phe Val Glu Thr         115 120 125 Ile Leu Phe Ala Ser Val Thr Ser Leu Ser Ile Glu Val Leu Gln Trp     130 135 140 Val Phe Ala Ile Gly Tyr Ser Asp Val Asp Asp Leu Leu Phe Asn Thr 145 150 155 160 Ile Gly Gly Leu Ile Gly Ala Ser Val Ala Ala Leu Val Ser Leu Lys                 165 170 175 Ser Ser Lys Val Val Ser Gly Ile Met Gly Gly Ser Leu Ser Val             180 185 190 Met Ala Met Met Met Tyr Ser Ser Phe Ile Ala         195 200 <210> 205 <211> 1359 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (1336) <223> RXN01873 <400> 205 ccgtcgttgc ccatggtcac agcctacatg cacaaagtga atcaaaaaca gctatttcta 60 acattttact aatatttgct gttggcgcat gatgaactcc atg agc caa gca ata 115                                             Met Ser Gln Ala Ile                                               1 5 gat agc aag gtc gag gca cac gaa ggc cac gaa ggc cac gaa ggc atc 163 Asp Ser Lys Val Glu Ala His Glu Gly His Glu Gly His Glu Gly Ile                  10 15 20 gag cga gga aca cgc aat tac aag cgc gct gtg ttt gcg atg ctg gcc 211 Glu Arg Gly Thr Arg Asn Tyr Lys Arg Ala Val Phe Ala Met Leu Ala              25 30 35 gcc ggt ctt gct gct ttc aat ggt ctt tat tgc acg cag gca ttg ctt 259 Ala Gly Leu Ala Ala Phe Asn Gly Leu Tyr Cys Thr Gln Ala Leu Leu          40 45 50 ccc acc atg acg gaa gag ttg gga att acg ccc act gag tcc gcg ctg 307 Pro Thr Met Thr Glu Glu Leu Gly Ile Thr Pro Thr Glu Ser Ala Leu      55 60 65 acg gtg tcg gct acg act gga atg ttg gcg ctg tgt att gtt ccg gcg 355 Thr Val Ser Ala Thr Thr Gly Met Leu Ala Leu Cys Ile Val Pro Ala  70 75 80 85 tcg ata ctt tcg gag aaa ttt ggt cgc ggt cgg gtg ctg aca att tca 403 Ser Ile Leu Ser Glu Lys Phe Gly Arg Gly Arg Val Leu Thr Ile Ser                  90 95 100 ctc acg ttg gcc atc atc gtg gga tta att ttg ccg ctt gtc ccc aat 451 Leu Thr Leu Ala Ile Ile Val Gly Leu Ile Leu Pro Leu Val Pro Asn             105 110 115 att act gct ctc atc ctg ctc aga ggt ctc caa ggt gcg ctg ctt gct 499 Ile Thr Ala Leu Ile Leu Leu Arg Gly Leu Gln Gly Ala Leu Leu Ala         120 125 130 ggc act cca gcg gtg gcg atg acc tgg ttg tct gag gaa att cac ccc 547 Gly Thr Pro Ala Val Ala Met Thr Trp Leu Ser Glu Glu Ile His Pro     135 140 145 aag gat att ggg cat gcg atg gga att tac atc gcg gga aat act gtc 595 Lys Asp Ile Gly His Ala Met Gly Ile Tyr Ile Ala Gly Asn Thr Val 150 155 160 165 ggc ggg ctc act gga cgt atg att ccg gcg gga cta ctt gaa gta act 643 Gly Gly Leu Thr Gly Arg Met Ile Pro Ala Gly Leu Leu Glu Val Thr                 170 175 180 cat tgg caa aac gca ctg ctg gga agt tct atc gct gcg ctg atc ttc 691 His Trp Gln Asn Ala Leu Leu Gly Ser Ser Ile Ala Ala Leu Ile Phe             185 190 195 ggc gta atc atg gtg gtg ttg ctt ccc aag cag cgg aaa ttc cag ccg 739 Gly Val Ile Met Val Val Leu Leu Pro Lys Gln Arg Lys Phe Gln Pro         200 205 210 aag aat atc aat ctg cgc cat gag att tcg gcg atg gct gct cat tgg 787 Lys Asn Ile Asn Leu Arg His Glu Ile Ser Ala Met Ala Ala His Trp     215 220 225 cgg aat cct cgt ttg gcg ttg ctt ttt ggt act gcg ttt ttg ggc atg 835 Arg Asn Pro Arg Leu Ala Leu Leu Phe Gly Thr Ala Phe Leu Gly Met 230 235 240 245 ggt act ttt gtg tcg ctg tac aac tat ttg ggt ttc cgc atg att gat 883 Gly Thr Phe Val Ser Leu Tyr Asn Tyr Leu Gly Phe Arg Met Ile Asp                 250 255 260 cag ttt ggg ctg agt gaa gtg ctg gtt ggt gcg gtg ttc atc atg tat 931 Gln Phe Gly Leu Ser Glu Val Leu Val Gly Ala Val Phe Ile Met Tyr             265 270 275 ctg gcc ggg acc tgg agt tcc acc cag gcg ggt gcg ttg agg gag aag 979 Leu Ala Gly Thr Trp Ser Ser Thr Gln Ala Gly Ala Leu Arg Glu Lys         280 285 290 atc ggc aat ggg tca acg gtt att ttc ttg agt ctg acg atg atc gcg 1027 Ile Gly Asn Gly Ser Thr Val Ile Phe Leu Ser Leu Thr Met Ile Ala     295 300 305 tcg atg gca ctg atg ggg att aat aat ttg tgg gtc acg ttg gtt gcc 1075 Ser Met Ala Leu Met Gly Ile Asn Asn Leu Trp Val Thr Leu Val Ala 310 315 320 325 ctg ttt gtg ttt acc gcg gca ttt ttc gca ctg cat tcc agt gct tcg 1123 Leu Phe Val Phe Thr Ala Ala Phe Phe Ala Leu His Ser Ser Ala Ser                 330 335 340 gga tgg atc gga atc atc gca acg aag gat cgc gcg gaa gcc tcc agc 1171 Gly Trp Ile Gly Ile Ile Ala Thr Lys Asp Arg Ala Glu Ala Ser Ser             345 350 355 atg tat ttg ttc tgt tat tac gtg gga tcc tcg gtg att ggt tgg gtt 1219 Met Tyr Leu Phe Cys Tyr Tyr Val Gly Ser Ser Val Ile Gly Trp Val         360 365 370 tct gga ttc gcg ttt acg cat ttg ccg tgg ttg gcg ttc att ggc tgg 1267 Ser Gly Phe Ala Phe Thr His Leu Pro Trp Leu Ala Phe Ile Gly Trp     375 380 385 ttg att ctg ctt ctt tgc gga gtg ctg gcg att tgt gtg acg ctg gca 1315 Leu Ile Leu Leu Leu Cys Gly Val Leu Ala Ile Cys Val Thr Leu Ala 390 395 400 405 agg ctt gcc cgc aac gcc aat taatacgagt ttgtccgtgt tta 1359 Arg Leu Ala Arg Asn Ala Asn                 410 <206> 206 <211> 412 <212> PRT <213> Corynebacterium glutamicum <400> 206 Met Ser Gln Ala Ile Asp Ser Lys Val Glu Ala His Glu Gly His Glu   1 5 10 15 Gly His Glu Gly Ile Glu Arg Gly Thr Arg Asn Tyr Lys Arg Ala Val              20 25 30 Phe Ala Met Leu Ala Ala Gly Leu Ala Ala Phe Asn Gly Leu Tyr Cys          35 40 45 Thr Gln Ala Leu Leu Pro Thr Met Thr Glu Glu Leu Gly Ile Thr Pro      50 55 60 Thr Glu Ser Ala Leu Thr Val Ser Ala Thr Thr Gly Met Leu Ala Leu  65 70 75 80 Cys Ile Val Pro Ala Ser Ile Leu Ser Glu Lys Phe Gly Arg Gly Arg                  85 90 95 Val Leu Thr Ile Ser Leu Thr Leu Ala Ile Val Gly Leu Ile Leu             100 105 110 Pro Leu Val Pro Asn Ile Thr Ala Leu Ile Leu Leu Arg Gly Leu Gln         115 120 125 Gly Ala Leu Leu Ala Gly Thr Pro Ala Val Ala Met Thr Trp Leu Ser     130 135 140 Glu Glu Ile His Pro Lys Asp Ile Gly His Ala Met Gly Ile Tyr Ile 145 150 155 160 Ala Gly Asn Thr Val Gly Gly Leu Thr Gly Arg Met Ile Pro Ala Gly                 165 170 175 Leu Leu Glu Val Thr His Trp Gln Asn Ala Leu Leu Gly Ser Ser Ile             180 185 190 Ala Ala Leu Ile Phe Gly Val Ile Met Val Val Leu Leu Pro Lys Gln         195 200 205 Arg Lys Phe Gln Pro Lys Asn Ile Asn Leu Arg His Glu Ile Ser Ala     210 215 220 Met Ala Ala His Trp Arg Asn Pro Arg Leu Ala Leu Leu Phe Gly Thr 225 230 235 240 Ala Phe Leu Gly Met Gly Thr Phe Val Ser Leu Tyr Asn Tyr Leu Gly                 245 250 255 Phe Arg Met Ile Asp Gln Phe Gly Leu Ser Glu Val Leu Val Gly Ala             260 265 270 Val Phe Ile Met Tyr Leu Ala Gly Thr Trp Ser Ser Thr Gln Ala Gly         275 280 285 Ala Leu Arg Glu Lys Ile Gly Asn Gly Ser Thr Val Ile Phe Leu Ser     290 295 300 Leu Thr Met Ile Ala Ser Met Ala Leu Met Gly Ile Asn Asn Leu Trp 305 310 315 320 Val Thr Leu Val Ala Leu Phe Val Phe Thr Ala Ala Phe Phe Ala Leu                 325 330 335 His Ser Ser Ala Ser Gly Trp Ile Gly Ile Ile Ala Thr Lys Asp Arg             340 345 350 Ala Glu Ala Ser Ser Met Tyr Leu Phe Cys Tyr Tyr Val Gly Ser Ser         355 360 365 Val Ile Gly Trp Val Ser Gly Phe Ala Phe Thr His Leu Pro Trp Leu     370 375 380 Ala Phe Ile Gly Trp Leu Ile Leu Leu Leu Cys Gly Val Leu Ala Ile 385 390 395 400 Cys Val Thr Leu Ala Arg Leu Ala Arg Asn Ala Asn                 405 410 <210> 207 <211> 1215 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1192) <223> FRXA01873 <400> 207 ccgtcgttgc ccatggtcac agcctacatg cacaaagtga atcaaaaaca gctatttcta 60 acattttact aatatttgct gttggcgcat gatgaactcc atg agc caa gca ata 115                                             Met Ser Gln Ala Ile                                               1 5 gat agc aag gtc gag gca cac gaa ggc cac gaa ggc cac gaa ggc atc 163 Asp Ser Lys Val Glu Ala His Glu Gly His Glu Gly His Glu Gly Ile                  10 15 20 gag cga gga aca cgc aat tac aag cgc gct gtg ttt gcg atg ctg gcc 211 Glu Arg Gly Thr Arg Asn Tyr Lys Arg Ala Val Phe Ala Met Leu Ala              25 30 35 gcc ggt ctt gct gct ttc aat ggt ctt tat tgc acg cag gca ttg ctt 259 Ala Gly Leu Ala Ala Phe Asn Gly Leu Tyr Cys Thr Gln Ala Leu Leu          40 45 50 ccc acc atg acg gaa gag ttg gga att acg ccc act gag tcc gcg ctg 307 Pro Thr Met Thr Glu Glu Leu Gly Ile Thr Pro Thr Glu Ser Ala Leu      55 60 65 acg gtg tcg gct acg act gga atg ttg gcg ctg tgt att gtt ccg gcg 355 Thr Val Ser Ala Thr Thr Gly Met Leu Ala Leu Cys Ile Val Pro Ala  70 75 80 85 tcg ata ctt tcg gag aaa ttt ggt cgc ggt cgg gtg ctg aca att tca 403 Ser Ile Leu Ser Glu Lys Phe Gly Arg Gly Arg Val Leu Thr Ile Ser                  90 95 100 ctc acg ttg gcc atc atc gtg gga tta att ttg ccg ctt gtc ccc aat 451 Leu Thr Leu Ala Ile Ile Val Gly Leu Ile Leu Pro Leu Val Pro Asn             105 110 115 att act gct ctc atc ctg ctc aga ggt ctc caa ggt gcg ctg ctt gct 499 Ile Thr Ala Leu Ile Leu Leu Arg Gly Leu Gln Gly Ala Leu Leu Ala         120 125 130 ggc act cca gcg gtg gcg atg acc tgg ttg tct gag gaa att cac ccc 547 Gly Thr Pro Ala Val Ala Met Thr Trp Leu Ser Glu Glu Ile His Pro     135 140 145 aag gat att ggg cat gcg atg gga att tac atc gcg gga aat act gtc 595 Lys Asp Ile Gly His Ala Met Gly Ile Tyr Ile Ala Gly Asn Thr Val 150 155 160 165 ggc ggg ctc act gga cgt atg att ccg gcg gga cta ctt gaa gta act 643 Gly Gly Leu Thr Gly Arg Met Ile Pro Ala Gly Leu Leu Glu Val Thr                 170 175 180 cat tgg caa aac gca ctg ctg gga agt tct atc gct gcg ctg atc ttc 691 His Trp Gln Asn Ala Leu Leu Gly Ser Ser Ile Ala Ala Leu Ile Phe             185 190 195 ggc gta atc atg gtg gtg ttg ctt ccc aag cag cgg aaa ttc cag ccg 739 Gly Val Ile Met Val Val Leu Leu Pro Lys Gln Arg Lys Phe Gln Pro         200 205 210 aag aat atc aat ctg cgc cat gag att tcg gcg atg gct gct cat tgg 787 Lys Asn Ile Asn Leu Arg His Glu Ile Ser Ala Met Ala Ala His Trp     215 220 225 cgg aat cct cgt ttg gcg ttg ctt ttt ggt act gcg ttt ttg ggc atg 835 Arg Asn Pro Arg Leu Ala Leu Leu Phe Gly Thr Ala Phe Leu Gly Met 230 235 240 245 ggt act ttt gtg tcg ctg tac aac tat ttg ggt ttc cgc atg att gat 883 Gly Thr Phe Val Ser Leu Tyr Asn Tyr Leu Gly Phe Arg Met Ile Asp                 250 255 260 cag ttt ggg ctg agt gaa gtg ctg gtt ggt gcg gtg ttc atc atg tat 931 Gln Phe Gly Leu Ser Glu Val Leu Val Gly Ala Val Phe Ile Met Tyr             265 270 275 ctg gcc ggg acc tgg agt tcc acc cag gcg ggt gcg ttg agg gag aag 979 Leu Ala Gly Thr Trp Ser Ser Thr Gln Ala Gly Ala Leu Arg Glu Lys         280 285 290 atc ggc aat ggg tca acg gtt att ttc ttg agt ctg acg atg atc gcg 1027 Ile Gly Asn Gly Ser Thr Val Ile Phe Leu Ser Leu Thr Met Ile Ala     295 300 305 tcg atg gca ctg atg ggg att aat aat ttg tgg gtc acg ttg gtt gcc 1075 Ser Met Ala Leu Met Gly Ile Asn Asn Leu Trp Val Thr Leu Val Ala 310 315 320 325 ctg ttt gtg ttt acc gcg gca ttt ttc gca ctg cat tcc agt gct tcg 1123 Leu Phe Val Phe Thr Ala Ala Phe Phe Ala Leu His Ser Ser Ala Ser                 330 335 340 gga tgg atc gga atc atc gca acg aag gat cgc gcg gaa gcc tcc agc 1171 Gly Trp Ile Gly Ile Ile Ala Thr Lys Asp Arg Ala Glu Ala Ser Ser             345 350 355 atg tat ttg ttc tgt gaa tac taggatcctc ggtgattggt tgg 1215 Met Tyr Leu Phe Cys Glu Tyr         360 <210> 208 <211> 364 <212> PRT <213> Corynebacterium glutamicum <400> 208 Met Ser Gln Ala Ile Asp Ser Lys Val Glu Ala His Glu Gly His Glu   1 5 10 15 Gly His Glu Gly Ile Glu Arg Gly Thr Arg Asn Tyr Lys Arg Ala Val              20 25 30 Phe Ala Met Leu Ala Ala Gly Leu Ala Ala Phe Asn Gly Leu Tyr Cys          35 40 45 Thr Gln Ala Leu Leu Pro Thr Met Thr Glu Glu Leu Gly Ile Thr Pro      50 55 60 Thr Glu Ser Ala Leu Thr Val Ser Ala Thr Thr Gly Met Leu Ala Leu  65 70 75 80 Cys Ile Val Pro Ala Ser Ile Leu Ser Glu Lys Phe Gly Arg Gly Arg                  85 90 95 Val Leu Thr Ile Ser Leu Thr Leu Ala Ile Val Gly Leu Ile Leu             100 105 110 Pro Leu Val Pro Asn Ile Thr Ala Leu Ile Leu Leu Arg Gly Leu Gln         115 120 125 Gly Ala Leu Leu Ala Gly Thr Pro Ala Val Ala Met Thr Trp Leu Ser     130 135 140 Glu Glu Ile His Pro Lys Asp Ile Gly His Ala Met Gly Ile Tyr Ile 145 150 155 160 Ala Gly Asn Thr Val Gly Gly Leu Thr Gly Arg Met Ile Pro Ala Gly                 165 170 175 Leu Leu Glu Val Thr His Trp Gln Asn Ala Leu Leu Gly Ser Ser Ile             180 185 190 Ala Ala Leu Ile Phe Gly Val Ile Met Val Val Leu Leu Pro Lys Gln         195 200 205 Arg Lys Phe Gln Pro Lys Asn Ile Asn Leu Arg His Glu Ile Ser Ala     210 215 220 Met Ala Ala His Trp Arg Asn Pro Arg Leu Ala Leu Leu Phe Gly Thr 225 230 235 240 Ala Phe Leu Gly Met Gly Thr Phe Val Ser Leu Tyr Asn Tyr Leu Gly                 245 250 255 Phe Arg Met Ile Asp Gln Phe Gly Leu Ser Glu Val Leu Val Gly Ala             260 265 270 Val Phe Ile Met Tyr Leu Ala Gly Thr Trp Ser Ser Thr Gln Ala Gly         275 280 285 Ala Leu Arg Glu Lys Ile Gly Asn Gly Ser Thr Val Ile Phe Leu Ser     290 295 300 Leu Thr Met Ile Ala Ser Met Ala Leu Met Gly Ile Asn Asn Leu Trp 305 310 315 320 Val Thr Leu Val Ala Leu Phe Val Phe Thr Ala Ala Phe Phe Ala Leu                 325 330 335 His Ser Ser Ala Ser Gly Trp Ile Gly Ile Ile Ala Thr Lys Asp Arg             340 345 350 Ala Glu Ala Ser Ser Met Tyr Leu Phe Cys Glu Tyr         355 360 <210> 209 <211> 1572 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1549) <223> RXN00034 <400> 209 taaattttgt ggcactcccc acatttctat caatctatag aaagtatgac ttaaagtcga 60 ttttgcaagt ttctatagat tgatagaaaa gggagtttag atg tct tac aca tct 115                                             Met Ser Tyr Thr Ser                                               1 5 ttt aaa ggc gat gat aaa gcc ctc atc ggc ata gtt tta tca gtt ctc 163 Phe Lys Gly Asp Asp Lys Ala Leu Ile Gly Ile Val Leu Ser Val Leu                  10 15 20 aca ttt tgg ctt ttt gct cag tca acc cta aat atc ggc cca gat atg 211 Thr Phe Trp Leu Phe Ala Gln Ser Thr Leu Asn Ile Gly Pro Asp Met              25 30 35 gca act gat tta ggg atg agc gat ggc acc atg aac ata gct gtc gtg 259 Ala Thr Asp Leu Gly Met Ser Asp Gly Thr Met Asn Ile Ala Val Val          40 45 50 gcc gcc gcg tta ttc tgt gga aca ttt atc gtc gca gcc ggc ggc atc 307 Ala Ala Ala Leu Phe Cys Gly Thr Phe Ile Val Ala Ala Gly Gly Ile      55 60 65 gca gat gtc ttt ggc cga gta cga atc atg atg att ggc aac atc ctt 355 Ala Asp Val Phe Gly Arg Val Arg Ile Met Met Ile Gly Asn Ile Leu  70 75 80 85 aac atc ctg gga tct ctc ctc atc gcc acg gca acg act tct tta gcc 403 Asn Ile Leu Gly Ser Leu Leu Ile Ala Thr Ala Thr Thr Ser Leu Ala                  90 95 100 acc caa atg gtg atc acc ggc cga gtt ctc caa gga ctg gca gca gcg 451 Thr Gln Met Val Ile Thr Gly Arg Val Leu Gln Gly Leu Ala Ala Ala             105 110 115 gcc atc atg tct gca tcc cta gca tta gtt aag aca tat tgg tta ggt 499 Ala Ile Met Ser Ala Ser Leu Ala Leu Val Lys Thr Tyr Trp Leu Gly         120 125 130 act gac cgc caa cga gca gtc tcc att tgg tcc att ggt tca tgg ggt 547 Thr Asp Arg Gln Arg Ala Val Ser Ile Trp Ser Ile Gly Ser Trp Gly     135 140 145 ggc acc gga ttc tgc gcg ctt ttc gcg ggt ctt gtt gta gca agc ccc 595 Gly Thr Gly Phe Cys Ala Leu Phe Ala Gly Leu Val Val Ala Ser Pro 150 155 160 165 ttt ggt tgg aga gga atc ttc gcc ctc tgc gcg atc gtc tcc atc gtt 643 Phe Gly Trp Arg Gly Ile Phe Ala Leu Cys Ala Ile Val Ser Ile Val                 170 175 180 gct att gcc ctt acc cgc cac atc ccg gaa tcc cgt ccg gct caa tcc 691 Ala Ile Ala Leu Thr Arg His Ile Pro Glu Ser Arg Pro Ala Gln Ser             185 190 195 att ggc atg cat ttg gat tgg agt ggc atc atc gtt ctt gcc ctc agt 739 Ile Gly Met His Leu Asp Trp Ser Gly Ile Ile Val Leu Ala Leu Ser         200 205 210 gtt cta tct ctt gaa ttg ttt att acc caa ggt gaa tca ctt ggc tgg 787 Val Leu Ser Leu Glu Leu Phe Ile Thr Gln Gly Glu Ser Leu Gly Trp     215 220 225 acg cac tgg atg acc tgg act ctc ctt gcc gtt tct ttg aca ttt ctt 835 Thr His Trp Met Thr Trp Thr Leu Leu Ala Val Ser Leu Thr Phe Leu 230 235 240 245 gca gtt ttc gtc ttc att gaa cgc atc gcc agc tgg cca gtt ctc gac 883 Ala Val Phe Val Phe Ile Glu Arg Ile Ala Ser Trp Pro Val Leu Asp                 250 255 260 ttc aac ctt ttc aaa gac cac gcc ttc agc ggt gcg acc atc acc aac 931 Phe Asn Leu Phe Lys Asp His Ala Phe Ser Gly Ala Thr Ile Thr Asn             265 270 275 ttc att atg agc gct act ggc gga gta gtt gcc gtt gtc atg tgg gtt 979 Phe Ile Met Ser Ala Thr Gly Gly Val Val Ala Val Val Met Trp Val         280 285 290 cag caa atg gga tgg ggt gtc tcc cca aca atc tcg gga ctc acc agc 1027 Gln Gln Met Gly Trp Gly Val Ser Pro Thr Ile Ser Gly Leu Thr Ser     295 300 305 atc ggc ttc gca gcc ttt gtc atc ctt ttc att cga gtt gga gaa aag 1075 Ile Gly Phe Ala Ala Phe Val Ile Leu Phe Ile Arg Val Gly Glu Lys 310 315 320 325 gcc atg cag aaa gtt ggc gcc cga gca gtg atc atc acc gct ggc atc 1123 Ala Met Gln Lys Val Gly Ala Arg Ala Val Ile Thr Ala Gly Ile                 330 335 340 ttg gta gcg acc gcg acc gcc ctc cta atg atc acc gcg gtc agc gag 1171 Leu Val Ala Thr Ala Thr Ala Leu Leu Met Ile Thr Ala Val Ser Glu             345 350 355 tca acg tac atc gtc atc tcc ctc gcc ggc ttc tcc ctt tat ggc ctt 1219 Ser Thr Tyr Ile Val Ile Ser Leu Ala Gly Phe Ser Leu Tyr Gly Leu         360 365 370 ggc ctc gga ctc ttc gcc acc cca gtc acc gat act gcg ctt gga aca 1267 Gly Leu Gly Leu Phe Ala Thr Pro Val Thr Asp Thr Ala Leu Gly Thr     375 380 385 ctt ccc aaa gac cgt acc ggc gct ggt gca ggt gta ttc aag atg tcc 1315 Leu Pro Lys Asp Arg Thr Gly Ala Gly Ala Gly Val Phe Lys Met Ser 390 395 400 405 tct tcc ctc ggc gca gca ctc ggc atc gca atc tcc act tca gtg ttc 1363 Ser Ser Leu Gly Ala Ala Leu Gly Ile Ala Ile Ser Thr Ser Val Phe                 410 415 420 ctc gca ctt cgc gac ggc acc tcc atc aac tcc gac gtc gca ctc gcc 1411 Leu Ala Leu Arg Asp Gly Thr Ser Ile Asn Ser Asp Val Ala Leu Ala             425 430 435 gga aca gtt tca ctt ggc atc aac gtt gta ttc gca gca aca gcc acc 1459 Gly Thr Val Ser Leu Gly Ile Asn Val Val Phe Ala Ala Thr Ala Thr         440 445 450 atc acc gca gca gtc ctt att cca aaa gcc gct ggc aaa gtc tca caa 1507 Ile Thr Ala Ala Val Leu Ile Pro Lys Ala Ala Gly Lys Val Ser Gln     455 460 465 acc agc atc acc ctt cct gag cca gct atc gct gta aaa atc 1549 Thr Ser Ile Thr Leu Pro Glu Pro Ala Ile Ala Val Lys Ile 470 475 480 taaaacttca ccaggacaga taa 1572 <210> 210 <211> 483 <212> PRT <213> Corynebacterium glutamicum <400> 210 Met Ser Tyr Thr Ser Phe Lys Gly Asp Asp Lys Ala Leu Ile Gly Ile   1 5 10 15 Val Leu Ser Val Leu Thr Phe Trp Leu Phe Ala Gln Ser Thr Leu Asn              20 25 30 Ile Gly Pro Asp Met Ala Thr Asp Leu Gly Met Ser Asp Gly Thr Met          35 40 45 Asn Ile Ala Val Val Ala Ala Ala Leu Phe Cys Gly Thr Phe Ile Val      50 55 60 Ala Ala Gly Gly Ile Ala Asp Val Phe Gly Arg Val Arg Ile Met Met  65 70 75 80 Ile Gly Asn Ile Leu Asn Ile Leu Gly Ser Leu Leu Ile Ala Thr Ala                  85 90 95 Thr Thr Ser Le Le Ala Thr Gln Met Val Ile Thr Gly Arg Val Leu Gln             100 105 110 Gly Leu Ala Ala Ala Ala Ile Met Ser Ala Ser Leu Ala Leu Val Lys         115 120 125 Thr Tyr Trp Leu Gly Thr Asp Arg Gln Arg Ala Val Ser Ile Trp Ser     130 135 140 Ile Gly Ser Trp Gly Gly Thr Gly Phe Cys Ala Leu Phe Ala Gly Leu 145 150 155 160 Val Val Ala Ser Pro Phe Gly Trp Arg Gly Ile Phe Ala Leu Cys Ala                 165 170 175 Ile Val Ser Ile Val Ala Ile Ala Leu Thr Arg His Ile Pro Glu Ser             180 185 190 Arg Pro Ala Gln Ser Ile Gly Met His Leu Asp Trp Ser Gly Ile Ile         195 200 205 Val Leu Ala Leu Ser Val Leu Ser Leu Glu Leu Phe Ile Thr Gln Gly     210 215 220 Glu Ser Leu Gly Trp Thr His Trp Met Thr Trp Thr Leu Leu Ala Val 225 230 235 240 Ser Leu Thr Phe Leu Ala Val Phe Val Phe Ile Glu Arg Ile Ala Ser                 245 250 255 Trp Pro Val Leu Asp Phe Asn Leu Phe Lys Asp His Ala Phe Ser Gly             260 265 270 Ala Thr Ile Thr Asn Phe Ile Met Ser Ala Thr Gly Gly Val Val Ala         275 280 285 Val Val Met Trp Val Gln Gln Met Gly Trp Gly Val Ser Pro Thr Ile     290 295 300 Ser Gly Leu Thr Ser Ile Gly Phe Ala Ala Phe Val Ile Leu Phe Ile 305 310 315 320 Arg Val Gly Glu Lys Ala Met Gln Lys Val Gly Ala Arg Ala Val Ile                 325 330 335 Ile Thr Ala Gly Ile Leu Val Ala Thr Ala Thr Ala Leu Leu Met Ile             340 345 350 Thr Ala Val Ser Glu Ser Thr Tyr Ile Val Ile Ser Leu Ala Gly Phe         355 360 365 Ser Leu Tyr Gly Leu Gly Leu Gly Leu Phe Ala Thr Pro Val Thr Asp     370 375 380 Thr Ala Leu Gly Thr Leu Pro Lys Asp Arg Thr Gly Ala Gly Ala Gly 385 390 395 400 Val Phe Lys Met Ser Ser Ser Leu Gly Ala Ala Leu Gly Ile Ala Ile                 405 410 415 Ser Thr Ser Val Phe Leu Ala Leu Arg Asp Gly Thr Ser Ile Asn Ser             420 425 430 Asp Val Ala Leu Ala Gly Thr Val Ser Leu Gly Ile Asn Val Val Phe         435 440 445 Ala Ala Thr Ala Thr Ile Thr Ala Ala Val Leu Ile Pro Lys Ala Ala     450 455 460 Gly Lys Val Ser Gln Thr Ser Ile Thr Leu Pro Glu Pro Ala Ile Ala 465 470 475 480 Val Lys Ile              <210> 211 <211> 1045 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101) .. (1045) <223> FRXA02273 <400> 211 taaattttgt ggcactcccc acatttctat caatctatag aaagtatgac ttaaagtcga 60 ttttgcaagt ttctatagat tgatagaaaa gggagtttag atg tct tac aca tct 115                                             Met Ser Tyr Thr Ser                                               1 5 ttt aaa ggc gat gat aaa gcc ctc atc ggc ata gtt tta tca gtt ctc 163 Phe Lys Gly Asp Asp Lys Ala Leu Ile Gly Ile Val Leu Ser Val Leu                  10 15 20 aca ttt tgg ctt ttt gct cag tca acc cta aat atc ggc cca gat atg 211 Thr Phe Trp Leu Phe Ala Gln Ser Thr Leu Asn Ile Gly Pro Asp Met              25 30 35 gca act gat tta ggg atg agc gat ggc acc atg aac ata gct gtc gtg 259 Ala Thr Asp Leu Gly Met Ser Asp Gly Thr Met Asn Ile Ala Val Val          40 45 50 gcc gcc gcg tta ttc tgt gga aca ttt atc gtc gca gcc ggc ggc atc 307 Ala Ala Ala Leu Phe Cys Gly Thr Phe Ile Val Ala Ala Gly Gly Ile      55 60 65 gca gat gtc ttt ggc cga gta cga atc atg atg att ggc aac atc ctt 355 Ala Asp Val Phe Gly Arg Val Arg Ile Met Met Ile Gly Asn Ile Leu  70 75 80 85 aac atc ctg gga tct ctc ctc atc gcc acg gca acg act tct tta gcc 403 Asn Ile Leu Gly Ser Leu Leu Ile Ala Thr Ala Thr Thr Ser Leu Ala                  90 95 100 acc caa atg gtg atc acc ggc cga gtt ctc caa gga ctg gca gca gcg 451 Thr Gln Met Val Ile Thr Gly Arg Val Leu Gln Gly Leu Ala Ala Ala             105 110 115 gcc atc atg tct gca tcc cta gca tta gtt aag aca tat tgg tta ggt 499 Ala Ile Met Ser Ala Ser Leu Ala Leu Val Lys Thr Tyr Trp Leu Gly         120 125 130 act gac cgc caa cga gca gtc tcc att tgg tcc att ggt tca tgg ggt 547 Thr Asp Arg Gln Arg Ala Val Ser Ile Trp Ser Ile Gly Ser Trp Gly     135 140 145 ggc acc gga ttc tgc gcg ctt ttc gcg ggt ctt gtt gta gca agc ccc 595 Gly Thr Gly Phe Cys Ala Leu Phe Ala Gly Leu Val Val Ala Ser Pro 150 155 160 165 ttt ggt tgg aga gga atc ttc gcc ctc tgc gcg atc gtc tcc atc gtt 643 Phe Gly Trp Arg Gly Ile Phe Ala Leu Cys Ala Ile Val Ser Ile Val                 170 175 180 gct att gcc ctt acc cgc cac atc ccg gaa tcc cgt ccg gct caa tcc 691 Ala Ile Ala Leu Thr Arg His Ile Pro Glu Ser Arg Pro Ala Gln Ser             185 190 195 att ggc atg cat ttg gat tgg agt ggc atc atc gtt ctt gcc ctc agt 739 Ile Gly Met His Leu Asp Trp Ser Gly Ile Ile Val Leu Ala Leu Ser         200 205 210 gtt cta tct ctt gaa ttg ttt att acc caa ggt gaa tca ctt ggc tgg 787 Val Leu Ser Leu Glu Leu Phe Ile Thr Gln Gly Glu Ser Leu Gly Trp     215 220 225 acg cac tgg atg acc tgg act ctc ctt gcc gtt tct ttg aca ttt ctt 835 Thr His Trp Met Thr Trp Thr Leu Leu Ala Val Ser Leu Thr Phe Leu 230 235 240 245 gca gtt ttc gtc ttc att gaa cgc atc gcc agc tgg cca gtt ctc gac 883 Ala Val Phe Val Phe Ile Glu Arg Ile Ala Ser Trp Pro Val Leu Asp                 250 255 260 ttc aac ctt ttc aaa gac cac gcc ttc agc ggt gcg acc atc acc aac 931 Phe Asn Leu Phe Lys Asp His Ala Phe Ser Gly Ala Thr Ile Thr Asn             265 270 275 ttc att atg agc gct act ggc gga gta gtt gcc gtt gtc atg tgg gtt 979 Phe Ile Met Ser Ala Thr Gly Gly Val Val Ala Val Val Met Trp Val         280 285 290 cag caa atg gga tgg ggt gtc tcc cca aca atc tcg gga ctc acc agc 1027 Gln Gln Met Gly Trp Gly Val Ser Pro Thr Ile Ser Gly Leu Thr Ser     295 300 305 atc ggc ttc gca gcc ttt 1045 Ile Gly Phe Ala Ala Phe 310 315 <210> 212 <211> 315 <212> PRT <213> Corynebacterium glutamicum <400> 212 Met Ser Tyr Thr Ser Phe Lys Gly Asp Asp Lys Ala Leu Ile Gly Ile   1 5 10 15 Val Leu Ser Val Leu Thr Phe Trp Leu Phe Ala Gln Ser Thr Leu Asn              20 25 30 Ile Gly Pro Asp Met Ala Thr Asp Leu Gly Met Ser Asp Gly Thr Met          35 40 45 Asn Ile Ala Val Val Ala Ala Ala Leu Phe Cys Gly Thr Phe Ile Val      50 55 60 Ala Ala Gly Gly Ile Ala Asp Val Phe Gly Arg Val Arg Ile Met Met  65 70 75 80 Ile Gly Asn Ile Leu Asn Ile Leu Gly Ser Leu Leu Ile Ala Thr Ala                  85 90 95 Thr Thr Ser Le Le Ala Thr Gln Met Val Ile Thr Gly Arg Val Leu Gln             100 105 110 Gly Leu Ala Ala Ala Ala Ile Met Ser Ala Ser Leu Ala Leu Val Lys         115 120 125 Thr Tyr Trp Leu Gly Thr Asp Arg Gln Arg Ala Val Ser Ile Trp Ser     130 135 140 Ile Gly Ser Trp Gly Gly Thr Gly Phe Cys Ala Leu Phe Ala Gly Leu 145 150 155 160 Val Val Ala Ser Pro Phe Gly Trp Arg Gly Ile Phe Ala Leu Cys Ala                 165 170 175 Ile Val Ser Ile Val Ala Ile Ala Leu Thr Arg His Ile Pro Glu Ser             180 185 190 Arg Pro Ala Gln Ser Ile Gly Met His Leu Asp Trp Ser Gly Ile Ile         195 200 205 Val Leu Ala Leu Ser Val Leu Ser Leu Glu Leu Phe Ile Thr Gln Gly     210 215 220 Glu Ser Leu Gly Trp Thr His Trp Met Thr Trp Thr Leu Leu Ala Val 225 230 235 240 Ser Leu Thr Phe Leu Ala Val Phe Val Phe Ile Glu Arg Ile Ala Ser                 245 250 255 Trp Pro Val Leu Asp Phe Asn Leu Phe Lys Asp His Ala Phe Ser Gly             260 265 270 Ala Thr Ile Thr Asn Phe Ile Met Ser Ala Thr Gly Gly Val Val Ala         275 280 285 Val Val Met Trp Val Gln Gln Met Gly Trp Gly Val Ser Pro Thr Ile     290 295 300 Ser Gly Leu Thr Ser Ile Gly Phe Ala Ala Phe 305 310 315 <210> 213 <211> 826 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (826) <223> RXN03075 <400> 213 tgtgcaaaat tgcattcagg ctgaaaaatt cctaaaggga ctccgtccga ataattggaa 60 agcccagaag aacagtcaac tcctagatta aaggataatc gtg gcg aaa ttc ctg 115                                             Val Ala Lys Phe Leu                                               1 5 tat aag tta ggc tcc acg gcc tat caa aag aaa tgg ccg ttt ctt gcg 163 Tyr Lys Leu Gly Ser Thr Ala Tyr Gln Lys Lys Trp Pro Phe Leu Ala                  10 15 20 gtc tgg ctc gtg att ctc ata ggt atc acg acg ctg gcg ggg ctg tat 211 Val Trp Leu Val Ile Leu Ile Gly Ile Thr Thr Leu Ala Gly Leu Tyr              25 30 35 gcc aag cca acg tcg agt agc ttc tct atc cct ggt ctt gat tct gtc 259 Ala Lys Pro Thr Ser Ser Ser Phe Ser Ile Pro Gly Leu Asp Ser Val          40 45 50 acg acc atg gag aag atg cag gag cgt ttc cct ggt tcg gat gat gca 307 Thr Thr Met Glu Lys Met Gln Glu Arg Phe Pro Gly Ser Asp Asp Ala      55 60 65 aca tcg gct ccc act ggt tct gtc gtc att cag gca ccg gaa ggc aag 355 Thr Ser Ala Pro Thr Gly Ser Val Val Ile Gln Ala Pro Glu Gly Lys  70 75 80 85 acc ctc act gat cct gag gtt ggg gct gaa gta aac cag atg ctt gat 403 Thr Leu Thr Asp Pro Glu Val Gly Ala Glu Val Asn Gln Met Leu Asp                  90 95 100 gag gtt cgg gcg act ggt gtg ctg aag gat gct gat tcc gtt gtg gat 451 Glu Val Arg Ala Thr Gly Val Leu Lys Asp Ala Asp Ser Val Val Asp             105 110 115 cct gtg ttg gct gcg cag ggt gtg gct gct cag atg acc cca gcc ctg 499 Pro Val Leu Ala Ala Gln Gly Val Ala Ala Gln Met Thr Pro Ala Leu         120 125 130 gag gct cag ggt gta cct gcg gag aag atc gcc gca gat att gag tcg 547 Glu Ala Gln Gly Val Pro Ala Glu Lys Ile Ala Ala Asp Ile Glu Ser     135 140 145 att agt cca ctg agt gca gat gag act acc ggc atc atc tcg atg act 595 Ile Ser Pro Leu Ser Ala Asp Glu Thr Thr Gly Ile Ile Ser Met Thr 150 155 160 165 ttt gat gca gat tct gcc atg gat ata tcc gca gag gat cgt gag aag 643 Phe Asp Ala Asp Ser Ala Met Asp Ile Ser Ala Glu Asp Arg Glu Lys                 170 175 180 gtc acc aat att ctt gat gaa tac gat gac ggc gat ctg act gtt gtc 691 Val Thr Asn Ile Leu Asp Glu Tyr Asp Asp Gly Asp Leu Thr Val Val             185 190 195 tac aac ggc aac gtg ttt ggc gca gct gca acc agc ttg gac atg acc 739 Tyr Asn Gly Asn Val Phe Gly Ala Ala Ala Thr Ser Leu Asp Met Thr         200 205 210 tct gag ctc atc ggc ctg ctg gtg gct gcg gtc gtt ctt atc gtg acc 787 Ser Glu Leu Ile Gly Leu Leu Val Ala Ala Val Val Leu Ile Val Thr     215 220 225 ttc ggt tcg ttc atc gct gcc ggt atg ccg ctg atc tct 826 Phe Gly Ser Phe Ile Ala Ala Gly Met Pro Leu Ile Ser 230 235 240 <210> 214 <211> 242 <212> PRT <213> Corynebacterium glutamicum <400> 214 Val Ala Lys Phe Leu Tyr Lys Leu Gly Ser Thr Ala Tyr Gln Lys Lys   1 5 10 15 Trp Pro Phe Leu Ala Val Trp Leu Val Ile Leu Ile Gly Ile Thr Thr              20 25 30 Leu Ala Gly Leu Tyr Ala Lys Pro Thr Ser Ser Ser Phe Ser Ile Pro          35 40 45 Gly Leu Asp Ser Val Thr Thr Met Glu Lys Met Gln Glu Arg Phe Pro      50 55 60 Gly Ser Asp Asp Ala Thr Ser Ala Pro Thr Gly Ser Val Val Ile Gln  65 70 75 80 Ala Pro Glu Gly Lys Thr Leu Thr Asp Pro Glu Val Gly Ala Glu Val                  85 90 95 Asn Gln Met Leu Asp Glu Val Arg Ala Thr Gly Val Leu Lys Asp Ala             100 105 110 Asp Ser Val Val Asp Pro Val Leu Ala Ala Gln Gly Val Ala Ala Gln         115 120 125 Met Thr Pro Ala Leu Glu Ala Gln Gly Val Pro Ala Glu Lys Ile Ala     130 135 140 Ala Asp Ile Glu Ser Ile Ser Pro Leu Ser Ala Asp Glu Thr Thr Gly 145 150 155 160 Ile Ile Ser Met Thr Phe Asp Ala Asp Ser Ala Met Asp Ile Ser Ala                 165 170 175 Glu Asp Arg Glu Lys Val Thr Asn Ile Leu Asp Glu Tyr Asp Asp Gly             180 185 190 Asp Leu Thr Val Val Tyr Asn Gly Asn Val Phe Gly Ala Ala Ala Thr         195 200 205 Ser Leu Asp Met Thr Ser Glu Leu Ile Gly Leu Leu Val Ala Ala Val     210 215 220 Val Leu Ile Val Thr Phe Gly Ser Phe Ile Ala Ala Gly Met Pro Leu 225 230 235 240 Ile ser          <210> 215 <211> 826 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (826) <223> FRXA02907 <400> 215 tgtgcaaaat tgcattcagg ctgaaaaatt cctaaaggga ctccgtccga ataattggaa 60 agcccagaag aacagtcaac tcctagatta aaggataatc gtg gcg aaa ttc ctg 115                                             Val Ala Lys Phe Leu                                               1 5 tat aag tta ggc tcc acg gcc tat caa aag aaa tgg ccg ttt ctt gcg 163 Tyr Lys Leu Gly Ser Thr Ala Tyr Gln Lys Lys Trp Pro Phe Leu Ala                  10 15 20 gtc tgg ctc gtg att ctc ata ggt atc acg acg ctg gcg ggg ctg tat 211 Val Trp Leu Val Ile Leu Ile Gly Ile Thr Thr Leu Ala Gly Leu Tyr              25 30 35 gcc aag cca acg tcg agt agc ttc tct atc cct ggt ctt gat tct gtc 259 Ala Lys Pro Thr Ser Ser Ser Phe Ser Ile Pro Gly Leu Asp Ser Val          40 45 50 acg acc atg gag aag atg cag gag cgt ttc cct ggt tcg gat gat gca 307 Thr Thr Met Glu Lys Met Gln Glu Arg Phe Pro Gly Ser Asp Asp Ala      55 60 65 aca tcg gct ccc act ggt tct gtc gtc att cag gca ccg gaa ggc aag 355 Thr Ser Ala Pro Thr Gly Ser Val Val Ile Gln Ala Pro Glu Gly Lys  70 75 80 85 acc ctc act gat cct gag gtt ggg gct gaa gta aac cag atg ctt gat 403 Thr Leu Thr Asp Pro Glu Val Gly Ala Glu Val Asn Gln Met Leu Asp                  90 95 100 gag gtt cgg gcg act ggt gtg ctg aag gat gct gat tcc gtt gtg gat 451 Glu Val Arg Ala Thr Gly Val Leu Lys Asp Ala Asp Ser Val Val Asp             105 110 115 cct gtg ttg gct gcg cag ggt gtg gct gct cag atg acc cca gcc ctg 499 Pro Val Leu Ala Ala Gln Gly Val Ala Ala Gln Met Thr Pro Ala Leu         120 125 130 gag gct cag ggt gta cct gcg gag aag atc gcc gca gat att gag tcg 547 Glu Ala Gln Gly Val Pro Ala Glu Lys Ile Ala Ala Asp Ile Glu Ser     135 140 145 att agt cca ctg agt gca gat gag act acc ggc atc atc tcg atg act 595 Ile Ser Pro Leu Ser Ala Asp Glu Thr Thr Gly Ile Ile Ser Met Thr 150 155 160 165 ttt gat gca gat tct gcc atg gat ata tcc gca gag gat cgt gag aag 643 Phe Asp Ala Asp Ser Ala Met Asp Ile Ser Ala Glu Asp Arg Glu Lys                 170 175 180 gtc acc aat att ctt gat gaa tac gat gac ggc gat ctg act gtt gtc 691 Val Thr Asn Ile Leu Asp Glu Tyr Asp Asp Gly Asp Leu Thr Val Val             185 190 195 tac aac ggc aac gtg ttt ggc gca gct gca acc agc ttg gac atg acc 739 Tyr Asn Gly Asn Val Phe Gly Ala Ala Ala Thr Ser Leu Asp Met Thr         200 205 210 tct gag ctc atc ggc ctg ctg gtg gct gcg gtc gtt ctt atc gtg acc 787 Ser Glu Leu Ile Gly Leu Leu Val Ala Ala Val Val Leu Ile Val Thr     215 220 225 ttc ggt tcg ttc atc gct gcc ggt atg ccg ctg atc tct 826 Phe Gly Ser Phe Ile Ala Ala Gly Met Pro Leu Ile Ser 230 235 240 <210> 216 <211> 242 <212> PRT <213> Corynebacterium glutamicum <400> 216 Val Ala Lys Phe Leu Tyr Lys Leu Gly Ser Thr Ala Tyr Gln Lys Lys   1 5 10 15 Trp Pro Phe Leu Ala Val Trp Leu Val Ile Leu Ile Gly Ile Thr Thr              20 25 30 Leu Ala Gly Leu Tyr Ala Lys Pro Thr Ser Ser Ser Phe Ser Ile Pro          35 40 45 Gly Leu Asp Ser Val Thr Thr Met Glu Lys Met Gln Glu Arg Phe Pro      50 55 60 Gly Ser Asp Asp Ala Thr Ser Ala Pro Thr Gly Ser Val Val Ile Gln  65 70 75 80 Ala Pro Glu Gly Lys Thr Leu Thr Asp Pro Glu Val Gly Ala Glu Val                  85 90 95 Asn Gln Met Leu Asp Glu Val Arg Ala Thr Gly Val Leu Lys Asp Ala             100 105 110 Asp Ser Val Val Asp Pro Val Leu Ala Ala Gln Gly Val Ala Ala Gln         115 120 125 Met Thr Pro Ala Leu Glu Ala Gln Gly Val Pro Ala Glu Lys Ile Ala     130 135 140 Ala Asp Ile Glu Ser Ile Ser Pro Leu Ser Ala Asp Glu Thr Thr Gly 145 150 155 160 Ile Ile Ser Met Thr Phe Asp Ala Asp Ser Ala Met Asp Ile Ser Ala                 165 170 175 Glu Asp Arg Glu Lys Val Thr Asn Ile Leu Asp Glu Tyr Asp Asp Gly             180 185 190 Asp Leu Thr Val Val Tyr Asn Gly Asn Val Phe Gly Ala Ala Ala Thr         195 200 205 Ser Leu Asp Met Thr Ser Glu Leu Ile Gly Leu Leu Val Ala Ala Val     210 215 220 Val Leu Ile Val Thr Phe Gly Ser Phe Ile Ala Ala Gly Met Pro Leu 225 230 235 240 Ile ser          <210> 217 <211> 2313 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (2290) <223> RXA00479 <400> 217 tagatcccaa ggctcaaaat ttattactta aacaagttga gcaactagcc agccgcaaat 60 cttagaacta acctttacgc ctttaacgga agtgaatttg atg tct act agc atc 115                                             Met Ser Thr Ser Ile                                               1 5 aca aca gag aac aag aag aaa tct ggt cct cct cgc ttg atg aga atc 163 Thr Thr Glu Asn Lys Lys Lys Ser Gly Pro Pro Arg Leu Met Arg Ile                  10 15 20 ttt ctg ccc gcc ttg cta att tta gtt tgg ctt gta gga gct gga gtc 211 Phe Leu Pro Ala Leu Leu Ile Leu Val Trp Leu Val Gly Ala Gly Val              25 30 35 ggc ggt cct tat ttt ggc aag gtt agt gag gtc tcc tcc aac agc cag 259 Gly Gly Pro Tyr Phe Gly Lys Val Ser Glu Val Ser Ser Asn Ser Gln          40 45 50 acc aca tat ctg cca gaa tct gcc gat gcc act caa gta cag gaa cag 307 Thr Thr Tyr Leu Pro Glu Ser Ala Asp Ala Thr Gln Val Gln Glu Gln      55 60 65 ttg gga gat ttt act gat tct gaa tcc atc cca gcc att gtc gta atg 355 Leu Gly Asp Phe Thr Asp Ser Glu Ser Ile Pro Ala Ile Val Val Met  70 75 80 85 gtc agc gat gaa ccc tta aca cag caa gac atc aca caa ctc aat gaa 403 Val Ser Asp Glu Pro Leu Thr Gln Gln Asp Ile Thr Gln Leu Asn Glu                  90 95 100 gtt gtt gct ggg ctt tca gaa tta gac ata gtt tcc gat gaa gtc tcc 451 Val Val Ala Gly Leu Ser Glu Leu Asp Ile Val Ser Asp Glu Val Ser             105 110 115 cct gct att cca tcc gag gac ggc aga gct gtc caa gtg ttt gtc ccc 499 Pro Ala Ile Pro Ser Glu Asp Gly Arg Ala Val Gln Val Phe Val Pro         120 125 130 ctc aat cca tca gcg gag ctg acg gaa agc gtc gag aag ctc tct gag 547 Leu Asn Pro Ser Ala Glu Leu Thr Glu Ser Val Glu Lys Leu Ser Glu     135 140 145 acc ttg acc cag caa acg ccg gac tat gtg agc acc tat gtg acc gga 595 Thr Leu Thr Gln Gln Thr Pro Asp Tyr Val Ser Thr Tyr Val Thr Gly 150 155 160 165 ccg gct ggg ttt acc gct gat ctc agc gca gct ttc gcg ggt att gat 643 Pro Ala Gly Phe Thr Ala Asp Leu Ser Ala Ala Phe Ala Gly Ile Asp                 170 175 180 ggg cta ctc cta gca gtc gcc ttg gct gcc gtc ctt gtc att ctt gtc 691 Gly Leu Leu Leu Ala Val Ala Leu Ala Ala Val Leu Val Ile Leu Val             185 190 195 atc gtc tat cgc tcc ttc att ctg ccc atc gcc gtg ctt gcc acc agt 739 Ile Val Tyr Arg Ser Phe Ile Leu Pro Ile Ala Val Leu Ala Thr Ser         200 205 210 ttg ttt gcg ctg act gta gct cta ttg gtg gtg tgg tgg cta gct aag 787 Leu Phe Ala Leu Thr Val Ala Leu Leu Val Val Trp Trp Leu Ala Lys     215 220 225 tgg gac atc ctg ctg ctt tcg ggt cag act caa ggc atc ctc ttc att 835 Trp Asp Ile Leu Leu Leu Ser Gly Gln Thr Gln Gly Ile Leu Phe Ile 230 235 240 245 ctg gtc att ggc gcc gcc acc gac tac tca ttg cta tac gtt gct cgt 883 Leu Val Ile Gly Ala Ala Thr Asp Tyr Ser Leu Leu Tyr Val Ala Arg                 250 255 260 ttc cgt gaa gag tta cgc gtt caa caa gat aaa ggg ata gcc aca ggg 931 Phe Arg Glu Glu Leu Arg Val Gln Gln Asp Lys Gly Ile Ala Thr Gly             265 270 275 aaa gcc atc cgg gca tcg gtg gaa ccc att ctt gcc tcg ggc agc act 979 Lys Ala Ile Arg Ala Ser Val Glu Pro Ile Leu Ala Ser Gly Ser Thr         280 285 290 gtt att gcg ggc ctc ctt tgt ttg cta ttt agt gat ttg aaa tct aac 1027 Val Ile Ala Gly Leu Leu Cys Leu Leu Phe Ser Asp Leu Lys Ser Asn     295 300 305 tcc acg cta ggt cca gta gct tcg gtg ggc att att ttt gca atg ctt 1075 Ser Thr Leu Gly Pro Val Ala Ser Val Gly Ile Ile Phe Ala Met Leu 310 315 320 325 tct gct ctt act ctg cta cca gcc ctg ctg ttt gta ttc ggt cgg gtg 1123 Ser Ala Leu Thr Leu Leu Pro Ala Leu Leu Phe Val Phe Gly Arg Val                 330 335 340 gcc ttt tgg ccc aag cga cca aaa tac gaa cct gaa aaa gcc cgt gcg 1171 Ala Phe Trp Pro Lys Arg Pro Lys Tyr Glu Pro Glu Lys Ala Arg Ala             345 350 355 aaa aac gac atc ccc gcc agc ggg atc tgg tca aaa gtg gct gat tta 1219 Lys Asn Asp Ile Pro Ala Ser Gly Ile Trp Ser Lys Val Ala Asp Leu         360 365 370 gtg gag cag cat cct cgt gca atc tgg gta tct aca ctt att gtg ctt 1267 Val Glu Gln His Pro Arg Ala Ile Trp Val Ser Thr Leu Ile Val Leu     375 380 385 ctc ttg ggt gcg gct ttc gtt ccc aca cta aaa gcg gac ggt gtg tcc 1315 Leu Leu Gly Ala Ala Phe Val Pro Thr Leu Lys Ala Asp Gly Val Ser 390 395 400 405 caa tcc gac cta gtt ctg ggt tcc tct gaa gca cgt gat ggc cag cag 1363 Gln Ser Asp Leu Val Leu Gly Ser Ser Glu Ala Arg Asp Gly Gln Gln                 410 415 420 gct tta ggc gaa cac ttc ccc ggt gga tcc ggc agt cct gct tat att 1411 Ala Leu Gly Glu His Phe Pro Gly Gly Ser Gly Ser Pro Ala Tyr Ile             425 430 435 atc gtt gat gaa aca cag gca gca cag gct gct gac gta gtc ctt aac 1459 Ile Val Asp Glu Thr Gln Ala Ala Gln Ala Ala Asp Val Val Leu Asn         440 445 450 aac gac aat ttc gag act gta act gta act agt gct gac tcc ccc tct 1507 Asn Asp Asn Phe Glu Thr Val Thr Val Thr Ser Ala Asp Ser Pro Ser     455 460 465 ggc tca gcc cca atc acc gct gac ggt att gtg ccg tta ggt tct ggt 1555 Gly Ser Ala Pro Ile Thr Ala Asp Gly Ile Val Pro Leu Gly Ser Gly 470 475 480 485 aca gct cca ggc ccg gta gtt gta gaa ggg caa gtc ctt tta caa gca 1603 Thr Ala Pro Gly Pro Val Val Val Glu Gly Gln Val Leu Leu Gln Ala                 490 495 500 aca ctt gtc gaa gca cca gat tcc gaa gaa gct caa aaa gct att cgc 1651 Thr Leu Val Glu Ala Pro Asp Ser Glu Glu Ala Gln Lys Ala Ile Arg             505 510 515 agt atc cgc caa act ttt gca gat gaa aat ata tca gcg gta gta ggc 1699 Ser Ile Arg Gln Thr Phe Ala Asp Glu Asn Ile Ser Ala Val Val Gly         520 525 530 ggt gtc act gca act tcc gta gac act aac gat gcc tcc atc cat gac 1747 Gly Val Thr Ala Thr Ser Val Asp Thr Asn Asp Ala Ser Ile His Asp     535 540 545 cgc aac ctg atc atc cca att gta ttg ctg gtc att ttg gtt att ctc 1795 Arg Asn Leu Ile Ile Pro Ile Val Leu Leu Val Ile Leu Val Ile Leu 550 555 560 565 atg ctg ttg ctg cgg tct att gtc gca cca ctc ctg cta gta gtc acc 1843 Met Leu Leu Leu Arg Ser Ile Val Ala Pro Leu Leu Leu Val Val Thr                 570 575 580 acc gtg gtg tct ttt gct act gct tta ggc gtg gct gct tta ctt ttc 1891 Thr Val Val Ser Phe Ala Thr Ala Leu Gly Val Ala Ala Leu Leu Phe             585 590 595 aat cac gtt ttc agt ttc cca gga gca gac ccc gca gta cct ctc tac 1939 Asn His Val Phe Ser Phe Pro Gly Ala Asp Pro Ala Val Pro Leu Tyr         600 605 610 gga ttt gta ttt tta gta gcc ttg ggc atc gac tac aac att ttc tta 1987 Gly Phe Val Phe Leu Val Ala Leu Gly Ile Asp Tyr Asn Ile Phe Leu     615 620 625 gtc acc cga atc cgt gaa gaa acc aaa acc cac ggc aca aga ctt gga 2035 Val Thr Arg Ile Arg Glu Glu Thr Lys Thr His Gly Thr Arg Leu Gly 630 635 640 645 att ctt cga ggc ctg aca gta acc ggc gga gta att acc tca gct gga 2083 Ile Leu Arg Gly Leu Thr Val Thr Gly Gly Val Ile Thr Ser Ala Gly                 650 655 660 gta gtt ctc gcc gca acg ttc gca gca ctc tat gtc atc cca att cta 2131 Val Val Leu Ala Ala Thr Phe Ala Ala Leu Tyr Val Ile Pro Ile Leu             665 670 675 ttc ctg gca caa att gcc ttc att gtc gct ttt gga gtt ctt att gat 2179 Phe Leu Ala Gln Ile Ala Phe Ile Val Ala Phe Gly Val Leu Ile Asp         680 685 690 acc ctg ctc gtt cgc gcc ttc ttg gtg cct gct ttg ttc tac gac atc 2227 Thr Leu Leu Val Arg Ala Phe Leu Val Pro Ala Leu Phe Tyr Asp Ile     695 700 705 gga ccg aaa atc tgg tgg ccg tca aaa ttg tcc aat cag aaa tac cag 2275 Gly Pro Lys Ile Trp Trp Pro Ser Lys Leu Ser Asn Gln Lys Tyr Gln 710 715 720 725 aag cag cct cag cta tgacacacca aaattcgcct ctc 2313 Lys Gln Pro Gln Leu                 730 <210> 218 <211> 730 <212> PRT <213> Corynebacterium glutamicum <400> 218 Met Ser Thr Ser Ile Thr Thr Glu Asn Lys Lys Lys Ser Gly Pro Pro   1 5 10 15 Arg Leu Met Arg Ile Phe Leu Pro Ala Leu Leu Ile Leu Val Trp Leu              20 25 30 Val Gly Ala Gly Val Gly Gly Pro Tyr Phe Gly Lys Val Ser Glu Val          35 40 45 Ser Ser Asn Ser Gln Thr Thr Tyr Leu Pro Glu Ser Ala Asp Ala Thr      50 55 60 Gln Val Gln Glu Gln Leu Gly Asp Phe Thr Asp Ser Glu Ser Ile Pro  65 70 75 80 Ala Ile Val Val Met Val Ser Asp Glu Pro Leu Thr Gln Gln Asp Ile                  85 90 95 Thr Gln Leu Asn Glu Val Val Ala Gly Leu Ser Glu Leu Asp Ile Val             100 105 110 Ser Asp Glu Val Ser Pro Ala Ile Pro Ser Glu Asp Gly Arg Ala Val         115 120 125 Gln Val Phe Val Pro Leu Asn Pro Ser Ala Glu Leu Thr Glu Ser Val     130 135 140 Glu Lys Leu Ser Glu Thr Leu Thr Gln Gln Thr Pro Asp Tyr Val Ser 145 150 155 160 Thr Tyr Val Thr Gly Pro Ala Gly Phe Thr Ala Asp Leu Ser Ala Ala                 165 170 175 Phe Ala Gly Ile Asp Gly Leu Leu Leu Ala Val Ala Leu Ala Ala Val             180 185 190 Leu Val Ile Leu Val Ile Val Tyr Arg Ser Phe Ile Leu Pro Ile Ala         195 200 205 Val Leu Ala Thr Ser Leu Phe Ala Leu Thr Val Ala Leu Leu Val Val     210 215 220 Trp Trp Leu Ala Lys Trp Asp Ile Leu Leu Leu Ser Gly Gln Thr Gln 225 230 235 240 Gly Ile Leu Phe Ile Leu Val Ile Gly Ala Ala Thr Asp Tyr Ser Leu                 245 250 255 Leu Tyr Val Ala Arg Phe Arg Glu Glu Leu Arg Val Gln Gln Asp Lys             260 265 270 Gly Ile Ala Thr Gly Lys Ala Ile Arg Ala Ser Val Glu Pro Ile Leu         275 280 285 Ala Ser Gly Ser Thr Val Ile Ala Gly Leu Leu Cys Leu Leu Phe Ser     290 295 300 Asp Leu Lys Ser Asn Ser Thr Leu Gly Pro Val Ala Ser Val Gly Ile 305 310 315 320 Ile Phe Ala Met Leu Ser Ala Leu Thr Leu Leu Pro Ala Leu Leu Phe                 325 330 335 Val Phe Gly Arg Val Ala Phe Trp Pro Lys Arg Pro Lys Tyr Glu Pro             340 345 350 Glu Lys Ala Arg Ala Lys Asn Asp Ile Pro Ala Ser Gly Ile Trp Ser         355 360 365 Lys Val Ala Asp Leu Val Glu Gln His Pro Arg Ala Ile Trp Val Ser     370 375 380 Thr Leu Ile Val Leu Leu Leu Gly Ala Ala Phe Val Pro Thr Leu Lys 385 390 395 400 Ala Asp Gly Val Ser Gln Ser Asp Leu Val Leu Gly Ser Ser Glu Ala                 405 410 415 Arg Asp Gly Gln Gln Ala Leu Gly Glu His Phe Pro Gly Gly Ser Gly             420 425 430 Ser Pro Ala Tyr Ile Ile Val Asp Glu Thr Gln Ala Ala Gln Ala Ala         435 440 445 Asp Val Val Leu Asn Asn Asp Asn Phe Glu Thr Val Thr Val Thr Ser     450 455 460 Ala Asp Ser Pro Ser Gly Ser Ala Pro Ile Thr Ala Asp Gly Ile Val 465 470 475 480 Pro Leu Gly Ser Gly Thr Ala Pro Gly Pro Val Val Val Glu Gly Gln                 485 490 495 Val Leu Leu Gln Ala Thr Leu Val Glu Ala Pro Asp Ser Glu Glu Ala             500 505 510 Gln Lys Ala Ile Arg Ser Ile Arg Gln Thr Phe Ala Asp Glu Asn Ile         515 520 525 Ser Ala Val Val Gly Gly Val Thr Ala Thr Ser Val Asp Thr Asn Asp     530 535 540 Ala Ser Ile His Asp Arg Asn Leu Ile Ile Pro Ile Val Leu Leu Val 545 550 555 560 Ile Leu Val Ile Leu Met Leu Leu Leu Arg Ser Ile Val Ala Pro Leu                 565 570 575 Leu Leu Val Val Thr Thr Thr Val Val Ser Phe Ala Thr Ala Leu Gly Val             580 585 590 Ala Ala Leu Leu Phe Asn His Val Phe Ser Phe Pro Gly Ala Asp Pro         595 600 605 Ala Val Pro Leu Tyr Gly Phe Val Phe Leu Val Ala Leu Gly Ile Asp     610 615 620 Tyr Asn Ile Phe Leu Val Thr Arg Ile Arg Glu Glu Thr Lys Thr His 625 630 635 640 Gly Thr Arg Leu Gly Ile Leu Arg Gly Leu Thr Val Thr Gly Gly Val                 645 650 655 Ile Thr Ser Ala Gly Val Val Leu Ala Ala Thr Phe Ala Ala Leu Tyr             660 665 670 Val Ile Pro Ile Leu Phe Leu Ala Gln Ile Ala Phe Ile Val Ala Phe         675 680 685 Gly Val Leu Ile Asp Thr Leu Leu Val Arg Ala Phe Leu Val Pro Ala     690 695 700 Leu Phe Tyr Asp Ile Gly Pro Lys Ile Trp Trp Pro Ser Lys Leu Ser 705 710 715 720 Asn Gln Lys Tyr Gln Lys Gln Pro Gln Leu                 725 730 <210> 219 <211> 983 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (1) .. (960) <223> RXN03124 <400> 219 atg act cct acc ctg gcg tcg atg att ggt ctg gct gtc ggt atc gac 48 Met Thr Pro Thr Leu Ala Ser Met Ile Gly Leu Ala Val Gly Ile Asp   1 5 10 15 tac gcg cta ttt atc gtg tcc cgt ttc cgc aat gag ttg att tct cag 96 Tyr Ala Leu Phe Ile Val Ser Arg Phe Arg Asn Glu Leu Ile Ser Gln              20 25 30 act ggc gct aat gat ctg gag cca aag gaa ttg gct gag cgt ctg cgc 144 Thr Gly Ala Asn Asp Leu Glu Pro Lys Glu Leu Ala Glu Arg Leu Arg          35 40 45 acc atg ccg ttg gct gct cgt gcg cat gcg atg gga atg gct gtg ggc 192 Thr Met Pro Leu Ala Ala Arg Ala His Ala Met Gly Met Ala Val Gly      50 55 60 act gcg ggt tct gcg gtt gta ttc gcg ggt acc acg gtg ctg atc gct 240 Thr Ala Gly Ser Ala Val Val Phe Ala Gly Thr Thr Val Leu Ile Ala  65 70 75 80 ctg gtt gct ctg tcg atc att aat att cca ttt cta acc gtg atg gcc 288 Leu Val Ala Leu Ser Ile Ile Asn Ile Pro Phe Leu Thr Val Met Ala                  85 90 95 att gct gcc gca atc acc gtt gcc atc gca gtt ctg gtt gct ctg tcc 336 Ile Ala Ala Ala Ile Thr Val Ala Ile Ala Val Leu Val Ala Leu Ser             100 105 110 ttc ctc cca gct ctg ctt ggc ctg ctt ggc act cgc atc ttc gca gca 384 Phe Leu Pro Ala Leu Leu Gly Leu Leu Gly Thr Arg Ile Phe Ala Ala         115 120 125 cgc gtg cct gga cct aag gtt ccg gat cct gag gac gag aag cca acg 432 Arg Val Pro Gly Pro Lys Val Pro Asp Pro Glu Asp Glu Lys Pro Thr     130 135 140 atg ggt ctg aag tgg gtc cgc ctt gtg cgc aag atg ccg gtg gct tac 480 Met Gly Leu Lys Trp Val Arg Leu Val Arg Lys Met Pro Val Ala Tyr 145 150 155 160 ctg ctg gtt ggc gtc gtt ttg ctt ggt gca atc gca att cct gcg acc 528 Leu Leu Val Gly Val Val Leu Leu Gly Ala Ile Ala Ile Pro Ala Thr                 165 170 175 aat atg cgc ctg gcc atg ccg act gat ggc acc tcc acg ctg ggc acc 576 Asn Met Arg Leu Ala Met Pro Thr Asp Gly Thr Ser Thr Leu Gly Thr             180 185 190 gcg ccg cgc acg ggg tat gac atg acg gca gat gcg ttc ggc ccg ggc 624 Ala Pro Arg Thr Gly Tyr Asp Met Thr Ala Asp Ala Phe Gly Pro Gly         195 200 205 cgc aac gcg ccc atg att gcg ctt atc gac gca acc gac gtc cct gag 672 Arg Asn Ala Pro Met Ile Ala Leu Ile Asp Ala Thr Asp Val Pro Glu     210 215 220 gaa gaa cgc cca ttg gtg ttt gga cag gcg gtg gag caa ttc ttg aac 720 Glu Glu Arg Pro Leu Val Phe Gly Gln Ala Val Glu Gln Phe Leu Asn 225 230 235 240 act gat ggt gtg aag aat gct cag atc act cag acc acg gag aat ttc 768 Thr Asp Gly Val Lys Asn Ala Gln Ile Thr Gln Thr Thr Glu Asn Phe                 245 250 255 gat acc gcg cag atc ctg tta ccc cag aat ttg atg cga tcg atg agc 816 Asp Thr Ala Gln Ile Leu Leu Pro Gln Asn Leu Met Arg Ser Met Ser             260 265 270 gca cct ctg aga ctc tcg caa ctc ttc gtg cag atg ctg aga cct tcg 864 Ala Pro Leu Arg Leu Ser Gln Leu Phe Val Gln Met Leu Arg Pro Ser         275 280 285 ctg atg aca ccg gcg cga cgt atg gca tta ctg gcg tca ccc caa ttt 912 Leu Met Thr Pro Ala Arg Arg Met Ala Leu Leu Ala Ser Pro Gln Phe     290 295 300 acg atg aca tct ctg ctc gcc tcg gcg acg tcc tgg ttc ctt acg ttc 960 Thr Met Thr Ser Leu Leu Ala Ser Ala Thr Ser Trp Phe Leu Thr Phe 305 310 315 320 tgatcgtttt ggttctagcg ttc 983 <210> 220 <211> 320 <212> PRT <213> Corynebacterium glutamicum <400> 220 Met Thr Pro Thr Leu Ala Ser Met Ile Gly Leu Ala Val Gly Ile Asp   1 5 10 15 Tyr Ala Leu Phe Ile Val Ser Arg Phe Arg Asn Glu Leu Ile Ser Gln              20 25 30 Thr Gly Ala Asn Asp Leu Glu Pro Lys Glu Leu Ala Glu Arg Leu Arg          35 40 45 Thr Met Pro Leu Ala Ala Arg Ala His Ala Met Gly Met Ala Val Gly      50 55 60 Thr Ala Gly Ser Ala Val Val Phe Ala Gly Thr Thr Val Leu Ile Ala  65 70 75 80 Leu Val Ala Leu Ser Ile Ile Asn Ile Pro Phe Leu Thr Val Met Ala                  85 90 95 Ile Ala Ala Ala Ile Thr Val Ala Ile Ala Val Leu Val Ala Leu Ser             100 105 110 Phe Leu Pro Ala Leu Leu Gly Leu Leu Gly Thr Arg Ile Phe Ala Ala         115 120 125 Arg Val Pro Gly Pro Lys Val Pro Asp Pro Glu Asp Glu Lys Pro Thr     130 135 140 Met Gly Leu Lys Trp Val Arg Leu Val Arg Lys Met Pro Val Ala Tyr 145 150 155 160 Leu Leu Val Gly Val Val Leu Leu Gly Ala Ile Ala Ile Pro Ala Thr                 165 170 175 Asn Met Arg Leu Ala Met Pro Thr Asp Gly Thr Ser Thr Leu Gly Thr             180 185 190 Ala Pro Arg Thr Gly Tyr Asp Met Thr Ala Asp Ala Phe Gly Pro Gly         195 200 205 Arg Asn Ala Pro Met Ile Ala Leu Ile Asp Ala Thr Asp Val Pro Glu     210 215 220 Glu Glu Arg Pro Leu Val Phe Gly Gln Ala Val Glu Gln Phe Leu Asn 225 230 235 240 Thr Asp Gly Val Lys Asn Ala Gln Ile Thr Gln Thr Thr Glu Asn Phe                 245 250 255 Asp Thr Ala Gln Ile Leu Leu Pro Gln Asn Leu Met Arg Ser Met Ser             260 265 270 Ala Pro Leu Arg Leu Ser Gln Leu Phe Val Gln Met Leu Arg Pro Ser         275 280 285 Leu Met Thr Pro Ala Arg Arg Met Ala Leu Leu Ala Ser Pro Gln Phe     290 295 300 Thr Met Thr Ser Leu Leu Ala Ser Ala Thr Ser Trp Phe Leu Thr Phe 305 310 315 320 <210> 221 <211> 762 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (1) .. (762) <223> FRXA01180 <400> 221 atg act cct acc ctg gcg tcg atg att ggt ctg gct gtc ggt atc gac 48 Met Thr Pro Thr Leu Ala Ser Met Ile Gly Leu Ala Val Gly Ile Asp   1 5 10 15 tac gcg cta ttt atc gtg tcc cgt ttc cgc aat gag ttg att tct cag 96 Tyr Ala Leu Phe Ile Val Ser Arg Phe Arg Asn Glu Leu Ile Ser Gln              20 25 30 act ggc gct aat gat ctg gag cca aag gaa ttg gct gag cgt ctg cgc 144 Thr Gly Ala Asn Asp Leu Glu Pro Lys Glu Leu Ala Glu Arg Leu Arg          35 40 45 acc atg ccg ttg gct gct cgt gcg cat gcg atg gga atg gct gtg ggc 192 Thr Met Pro Leu Ala Ala Arg Ala His Ala Met Gly Met Ala Val Gly      50 55 60 act gcg ggt tct gcg gtt gta ttc gcg ggt acc acg gtg ctg atc gct 240 Thr Ala Gly Ser Ala Val Val Phe Ala Gly Thr Thr Val Leu Ile Ala  65 70 75 80 ctg gtt gct ctg tcg atc att aat att cca ttt cta acc gtg atg gcc 288 Leu Val Ala Leu Ser Ile Ile Asn Ile Pro Phe Leu Thr Val Met Ala                  85 90 95 att gct gcc gca atc acc gtt gcc atc gca gtt ctg gtt gct ctg tcc 336 Ile Ala Ala Ala Ile Thr Val Ala Ile Ala Val Leu Val Ala Leu Ser             100 105 110 ttc ctc cca gct ctg ctt ggc ctg ctt ggc act cgc atc ttc gca gca 384 Phe Leu Pro Ala Leu Leu Gly Leu Leu Gly Thr Arg Ile Phe Ala Ala         115 120 125 cgc gtg cct gga cct aag gtt ccg gat cct gag gac gag aag cca acg 432 Arg Val Pro Gly Pro Lys Val Pro Asp Pro Glu Asp Glu Lys Pro Thr     130 135 140 atg ggt ctg aag tgg gtc cgc ctt gtg cgc aag atg ccg gtg gct tac 480 Met Gly Leu Lys Trp Val Arg Leu Val Arg Lys Met Pro Val Ala Tyr 145 150 155 160 ctg ctg gtt ggc gtc gtt ttg ctt ggt gca atc gca att cct gcg acc 528 Leu Leu Val Gly Val Val Leu Leu Gly Ala Ile Ala Ile Pro Ala Thr                 165 170 175 aat atg cgc ctg gcc atg ccg act gat ggc acc tcc acg ctg ggc acc 576 Asn Met Arg Leu Ala Met Pro Thr Asp Gly Thr Ser Thr Leu Gly Thr             180 185 190 gcg ccg cgc acg ggg tat gac atg acg gca gat gcg ttc ggc ccg ggc 624 Ala Pro Arg Thr Gly Tyr Asp Met Thr Ala Asp Ala Phe Gly Pro Gly         195 200 205 cgc aac gcg ccc atg att gcg ctt atc gac gca acc gac gtc cct gag 672 Arg Asn Ala Pro Met Ile Ala Leu Ile Asp Ala Thr Asp Val Pro Glu     210 215 220 gaa gaa cgc cca ttg gtg ttt gga cag gcg gtg gag caa ttc ttg aac 720 Glu Glu Arg Pro Leu Val Phe Gly Gln Ala Val Glu Gln Phe Leu Asn 225 230 235 240 act gat ggt gtg aag aat gct cag atc act cag acc acg gag 762 Thr Asp Gly Val Lys Asn Ala Gln Ile Thr Gln Thr Thr Glu                 245 250 <210> 222 <211> 254 <212> PRT <213> Corynebacterium glutamicum <400> 222 Met Thr Pro Thr Leu Ala Ser Met Ile Gly Leu Ala Val Gly Ile Asp   1 5 10 15 Tyr Ala Leu Phe Ile Val Ser Arg Phe Arg Asn Glu Leu Ile Ser Gln              20 25 30 Thr Gly Ala Asn Asp Leu Glu Pro Lys Glu Leu Ala Glu Arg Leu Arg          35 40 45 Thr Met Pro Leu Ala Ala Arg Ala His Ala Met Gly Met Ala Val Gly      50 55 60 Thr Ala Gly Ser Ala Val Val Phe Ala Gly Thr Thr Val Leu Ile Ala  65 70 75 80 Leu Val Ala Leu Ser Ile Ile Asn Ile Pro Phe Leu Thr Val Met Ala                  85 90 95 Ile Ala Ala Ala Ile Thr Val Ala Ile Ala Val Leu Val Ala Leu Ser             100 105 110 Phe Leu Pro Ala Leu Leu Gly Leu Leu Gly Thr Arg Ile Phe Ala Ala         115 120 125 Arg Val Pro Gly Pro Lys Val Pro Asp Pro Glu Asp Glu Lys Pro Thr     130 135 140 Met Gly Leu Lys Trp Val Arg Leu Val Arg Lys Met Pro Val Ala Tyr 145 150 155 160 Leu Leu Val Gly Val Val Leu Leu Gly Ala Ile Ala Ile Pro Ala Thr                 165 170 175 Asn Met Arg Leu Ala Met Pro Thr Asp Gly Thr Ser Thr Leu Gly Thr             180 185 190 Ala Pro Arg Thr Gly Tyr Asp Met Thr Ala Asp Ala Phe Gly Pro Gly         195 200 205 Arg Asn Ala Pro Met Ile Ala Leu Ile Asp Ala Thr Asp Val Pro Glu     210 215 220 Glu Glu Arg Pro Leu Val Phe Gly Gln Ala Val Glu Gln Phe Leu Asn 225 230 235 240 Thr Asp Gly Val Lys Asn Ala Gln Ile Thr Gln Thr Thr Glu                 245 250 <210> 223 <211> 393 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (370) <223> RXA02586 <400> 223 ttctctgaga tcgtcatgat gaagtacatc gcgttcggca tgatcgcagc gctgattctg 60 gatgccacca tcatccgcat gctgcttgtc ccccgccgtg atg cac ctg ctt cgc 115                                             Met His Leu Leu Arg                                               1 5 gac gac aac tgg tgg gca ccc ggc ttc gtt aaa aag gcc tac acc gtc 163 Asp Asp Asn Trp Trp Ala Pro Gly Phe Val Lys Lys Ala Tyr Thr Val                  10 15 20 atg ggt cac ggc tct gag gtg gag gaa gca cct cgc cca acc acc cgt 211 Met Gly His Gly Ser Glu Val Glu Glu Ala Pro Arg Pro Thr Thr Arg              25 30 35 cgc ctc aac gac gat gag gaa gtc acc gtg cat gaa gca gtt gtc gct 259 Arg Leu Asn Asp Asp Glu Glu Val Thr Val His Glu Ala Val Val Ala          40 45 50 ggc gat acc gtg gca tct cgc ggt ggt ttg agc acg cag gaa aac cgt 307 Gly Asp Thr Val Ala Ser Arg Gly Gly Leu Ser Thr Gln Glu Asn Arg      55 60 65 gat ctg gtg tcc ttc gtg gaa ctt aag gct cgt ttg gaa aag cgc agg 355 Asp Leu Val Ser Phe Val Glu Leu Lys Ala Arg Leu Glu Lys Arg Arg  70 75 80 85 ctt gag gat cta gat taaatctatg cgaggatttt tca 393 Leu Glu Asp Leu Asp                  90 <210> 224 <211> 90 <212> PRT <213> Corynebacterium glutamicum <400> 224 Met His Leu Leu Arg Asp Asp Asn Trp Trp Ala Pro Gly Phe Val Lys   1 5 10 15 Lys Ala Tyr Thr Val Met Gly His Gly Ser Glu Val Glu Glu Ala Pro              20 25 30 Arg Pro Thr Thr Arg Arg Leu Asn Asp Asp Glu Glu Val Thr Val His          35 40 45 Glu Ala Val Val Ala Gly Asp Thr Val Ala Ser Arg Gly Gly Leu Ser      50 55 60 Thr Gln Glu Asn Arg Asp Leu Val Ser Phe Val Glu Leu Lys Ala Arg  65 70 75 80 Leu Glu Lys Arg Arg Leu Glu Asp Leu Asp                  85 90 <210> 225 <211> 2214 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (2191) <223> RXA02587 <400> 225 agcctggata acctgccaga cggtggcgca tggctgcagc cgttccgccc tctgactgcc 60 ttgttatcca accgccacaa ttcccaggag taatccaccc gtg ttt tct aaa tgg 115                                             Val Phe Ser Lys Trp                                               1 5 ggc cac ttt gct tac aga ttt agg cgc att gtt ccg tta gtc gtc atc 163 Gly His Phe Ala Tyr Arg Phe Arg Arg Ile Val Pro Leu Val Val Ile                  10 15 20 gcc gcg att ttg gct ttg ttt gtc att ttc ggc acc aag ctg ggc gac 211 Ala Ala Ile Leu Ala Leu Phe Val Ile Phe Gly Thr Lys Leu Gly Asp              25 30 35 cgc atg agc cag gaa gga tgg gat gat cct ggt tct tcc tcg acc gct 259 Arg Met Ser Gln Glu Gly Trp Asp Asp Pro Gly Ser Ser Ser Thr Ala          40 45 50 gcg gcg cgc atc gag ttg gag acc ttt ggg cgt gac aat gac ggc gat 307 Ala Ala Arg Ile Glu Leu Glu Thr Phe Gly Arg Asp Asn Asp Gly Asp      55 60 65 gtc gtg ttg ctg ttt act gcg cct gaa ggc act tct ttc gat gat gca 355 Val Val Leu Leu Phe Thr Ala Pro Glu Gly Thr Ser Phe Asp Asp Ala  70 75 80 85 gag gtg ttc tcc agc atc tct ggc tac tta gat ggg cta atc gag aac 403 Glu Val Phe Ser Ser Ile Ser Gly Tyr Leu Asp Gly Leu Ile Glu Asn                  90 95 100 aac cct gat gaa gtc agc cac atc aac agc tac ttt gac act cgt aat 451 Asn Pro Asp Glu Val Ser His Ile Asn Ser Tyr Phe Asp Thr Arg Asn             105 110 115 caa aat ctc ctc agc aaa gac ggc acc caa acc ttt gca gct ctc ggg 499 Gln Asn Leu Leu Ser Lys Asp Gly Thr Gln Thr Phe Ala Ala Leu Gly         120 125 130 ctc aaa ggt gac ggc gag caa acg ctg aag gac ttc cgg gag att gaa 547 Leu Lys Gly Asp Gly Glu Gln Thr Leu Lys Asp Phe Arg Glu Ile Glu     135 140 145 gat cag ctc cat ccg gac aac ctt gcc ggt ggc gtc acc act gag gtc 595 Asp Gln Leu His Pro Asp Asn Leu Ala Gly Gly Val Thr Thr Glu Val 150 155 160 165 gcg ggt gcc acc gct gta gcc gac gca ctc gat gag ggc atg gct ggc 643 Ala Gly Ala Thr Ala Val Ala Asp Ala Leu Asp Glu Gly Met Ala Gly                 170 175 180 gat att tca cgc gcc gaa gtt ttt gcg ctg cct ttc gtg gct atc ttg 691 Asp Ile Ser Arg Ala Glu Val Phe Ala Leu Pro Phe Val Ala Ile Leu             185 190 195 ctg ctc atc gtg ttt ggc tca gtt gtt gcc gcg gcg atg cca ttg atc 739 Leu Leu Ile Val Phe Gly Ser Val Val Ala Ala Ala Met Pro Leu Ile         200 205 210 gtg ggc att ttg tcc atc ttg ggt tcg ctg ggc atc ttg gca att ttg 787 Val Gly Ile Leu Ser Ile Leu Gly Ser Leu Gly Ile Leu Ala Ile Leu     215 220 225 gct gga ttc ttc cag gtc aac gta ttt gca caa tct gtt gtg acc ctt 835 Ala Gly Phe Phe Gln Val Asn Val Phe Ala Gln Ser Val Val Thr Leu 230 235 240 245 ctg ggc ttg ggt ctt gcc att gac tat ggc tta ttc atg gtc tct cgt 883 Leu Gly Leu Gly Leu Ala Ile Asp Tyr Gly Leu Phe Met Val Ser Arg                 250 255 260 ttc cgt gag gaa atg gat aag ggc acc ccg gtt gaa cag gct gtt gcc 931 Phe Arg Glu Glu Met Asp Lys Gly Thr Pro Val Glu Gln Ala Val Ala             265 270 275 acc act acg gcg acc gcg ggt aag act gtg gtg ttc tct gca gcg atg 979 Thr Thr Thr Ala Thr Ala Gly Lys Thr Val Val Phe Ser Ala Ala Met         280 285 290 gtg gct gtg gcg ctg tcc ggg ttg ttt gtt ttc cca cag gct ttc ttg 1027 Val Ala Val Ala Leu Ser Gly Leu Phe Val Phe Pro Gln Ala Phe Leu     295 300 305 aag tcg gtg gca ttc ggt gcg att tcc gcg gtt ggc ctt gct gct ttg 1075 Lys Ser Val Ala Phe Gly Ala Ile Ser Ala Val Gly Leu Ala Ala Leu 310 315 320 325 atg tcg gtg acg gtg ttg ccg tcg ctg ttc agc atg ttg ggt aag aat 1123 Met Ser Val Thr Val Leu Pro Ser Leu Phe Ser Met Leu Gly Lys Asn                 330 335 340 atc gat aag tgg agt ttg cgt cgc act gct cga aca gcg cgc cgt ttg 1171 Ile Asp Lys Trp Ser Leu Arg Arg Thr Ala Arg Thr Ala Arg Arg Leu             345 350 355 gaa gac acc att tgg tac cgc gtg ccg gca tgg gca atg cgc cat gcc 1219 Glu Asp Thr Ile Trp Tyr Arg Val Pro Ala Trp Ala Met Arg His Ala         360 365 370 aag gca gtg acc gtg ggc gtc gta ttg ctc ttg ctt gct ctt aca gtg 1267 Lys Ala Val Thr Val Gly Val Val Leu Leu Leu Leu Ala Leu Thr Val     375 380 385 ccg ttg acg ggc gtg aaa ttc ggc ggc atc aat gaa acg tat ctg cca 1315 Pro Leu Thr Gly Val Lys Phe Gly Gly Ile Asn Glu Thr Tyr Leu Pro 390 395 400 405 cca gct aac gac acc cgc gtc gcc caa gag cgt ttc gac gag gcg ttt 1363 Pro Ala Asn Asp Thr Arg Val Ala Gln Glu Arg Phe Asp Glu Ala Phe                 410 415 420 ccc gcc ttc cgc acc gag ccg gtc aag ctt gtg gtc acc ggg gcg gac 1411 Pro Ala Phe Arg Thr Glu Pro Val Lys Leu Val Val Thr Gly Ala Asp             425 430 435 aac aac cag ctg atc gat atc tat gtt cag gcc aac gaa gtt gag gga 1459 Asn Asn Gln Leu Ile Asp Ile Tyr Val Gln Ala Asn Glu Val Glu Gly         440 445 450 ctg aca gat cgt ttc acc gca ggt gcg act acc gat gat ggc acc acg 1507 Leu Thr Asp Arg Phe Thr Ala Gly Ala Thr Thr Asp Asp Gly Thr Thr     455 460 465 gtg ttg tct act ggt att cag gat cgt tcc ctc aat gag cag gta gtg 1555 Val Leu Ser Thr Gly Ile Gln Asp Arg Ser Leu Asn Glu Gln Val Val 470 475 480 485 gag cag ctt cgc gct att tcc gtc cct gag ggc gtt gag gtg cag atc 1603 Glu Gln Leu Arg Ala Ile Ser Val Pro Glu Gly Val Glu Val Gln Ile                 490 495 500 ggt ggc act cca gcc atg gag atc gaa tcc att gag gcg ctc ttt gaa 1651 Gly Gly Thr Pro Ala Met Glu Ile Glu Ser Ile Glu Ala Leu Phe Glu             505 510 515 aag ctc ctc tgg atg gct ctc tac att gtg ctg gcc act ttc atc ctc 1699 Lys Leu Leu Trp Met Ala Leu Tyr Ile Val Leu Ala Thr Phe Ile Leu         520 525 530 atg gca ttg gta ttt ggt tcg gtg att ttg ccg gcg aag gcc atc atc 1747 Met Ala Leu Val Phe Gly Ser Val Ile Leu Pro Ala Lys Ala Ile Ile     535 540 545 atg acc att ctg ggt atg ggt gcc acc ttg ggt att ctc acc ttg atg 1795 Met Thr Ile Leu Gly Met Gly Ala Thr Leu Gly Ile Leu Thr Leu Met 550 555 560 565 ttc gtc gat ggc gtg ggt gcc agc gca ttg aac ttc tcc cct ggc cca 1843 Phe Val Asp Gly Val Gly Ala Ser Ala Leu Asn Phe Ser Pro Gly Pro                 570 575 580 ctg atg agt cca gtg ctg gtg ctg atc atg gct att att tac gga ctt 1891 Leu Met Ser Pro Val Leu Val Leu Ile Met Ala Ile Ile Tyr Gly Leu             585 590 595 tcc acc gac tat gag gtg ttc ctg gta tct cgc atg gtg gag gcc cgc 1939 Ser Thr Asp Tyr Glu Val Phe Leu Val Ser Arg Met Val Glu Ala Arg         600 605 610 gat aaa ggc gaa tcc acc gac gac gcc atc aga tac ggc act gca cac 1987 Asp Lys Gly Glu Ser Thr Asp Asp Ala Ile Arg Tyr Gly Thr Ala His     615 620 625 acc gga tct atc atc acc gcg gcc gca ctg atc atg att gtg gtc tgt 2035 Thr Gly Ser Ile Ile Thr Ala Ala Ala Leu Ile Met Ile Val Val Cys 630 635 640 645 gga gcg ttt ggt ttc tct gag atc gtc atg atg aag tac atc gcg ttc 2083 Gly Ala Phe Gly Phe Ser Glu Ile Val Met Met Lys Tyr Ile Ala Phe                 650 655 660 ggc atg atc gca gcg ctg att ctg gat gcc acc atc atc cgc atg ctg 2131 Gly Met Ile Ala Ala Leu Ile Leu Asp Ala Thr Ile Ile Arg Met Leu             665 670 675 ctt gtc ccc cgc cgt gat gca cct gct tcg cga cga caa ctg gtg ggc 2179 Leu Val Pro Arg Arg Asp Ala Pro Ala Ser Arg Arg Gln Leu Val Gly         680 685 690 acc cgg ctt cgt taaaaaggcc tacaccgtca tgg 2214 Thr Arg Leu Arg     695 <210> 226 <211> 697 <212> PRT <213> Corynebacterium glutamicum <400> 226 Val Phe Ser Lys Trp Gly His Phe Ala Tyr Arg Phe Arg Arg Ile Val   1 5 10 15 Pro Leu Val Val Ile Ala Ala Ile Leu Ala Leu Phe Val Ile Phe Gly              20 25 30 Thr Lys Leu Gly Asp Arg Met Ser Gln Glu Gly Trp Asp Asp Pro Gly          35 40 45 Ser Ser Ser Thr Ala Ala Ala Arg Ile Glu Leu Glu Thr Phe Gly Arg      50 55 60 Asp Asn Asp Gly Asp Val Val Leu Leu Phe Thr Ala Pro Glu Gly Thr  65 70 75 80 Ser Phe Asp Asp Ala Glu Val Phe Ser Ser Ile Ser Gly Tyr Leu Asp                  85 90 95 Gly Leu Ile Glu Asn Asn Pro Asp Glu Val Ser His Ile Asn Ser Tyr             100 105 110 Phe Asp Thr Arg Asn Gln Asn Leu Leu Ser Lys Asp Gly Thr Gln Thr         115 120 125 Phe Ala Ala Leu Gly Leu Lys Gly Asp Gly Glu Gln Thr Leu Lys Asp     130 135 140 Phe Arg Glu Ile Glu Asp Gln Leu His Pro Asp Asn Leu Ala Gly Gly 145 150 155 160 Val Thr Thr Glu Val Ala Gly Ala Thr Ala Val Ala Asp Ala Leu Asp                 165 170 175 Glu Gly Met Ala Gly Asp Ile Ser Arg Ala Glu Val Phe Ala Leu Pro             180 185 190 Phe Val Ala Ile Leu Leu Leu Ile Val Phe Gly Ser Val Val Ala Ala         195 200 205 Ala Met Pro Leu Ile Val Gly Ile Leu Ser Ile Leu Gly Ser Leu Gly     210 215 220 Ile Leu Ala Ile Leu Ala Gly Phe Phe Gln Val Asn Val Phe Ala Gln 225 230 235 240 Ser Val Val Thr Leu Leu Gly Leu Gly Leu Ala Ile Asp Tyr Gly Leu                 245 250 255 Phe Met Val Ser Arg Phe Arg Glu Glu Met Asp Lys Gly Thr Pro Val             260 265 270 Glu Gln Ala Val Ala Thr Thr Thr Ala Thr Ala Gly Lys Thr Val Val         275 280 285 Phe Ser Ala Ala Met Val Ala Val Ala Leu Ser Gly Leu Phe Val Phe     290 295 300 Pro Gln Ala Phe Leu Lys Ser Val Ala Phe Gly Ala Ile Ser Ala Val 305 310 315 320 Gly Leu Ala Ala Leu Met Ser Val Thr Val Leu Pro Ser Leu Phe Ser                 325 330 335 Met Leu Gly Lys Asn Ile Asp Lys Trp Ser Leu Arg Arg Thr Ala Arg             340 345 350 Thr Ala Arg Arg Leu Glu Asp Thr Ile Trp Tyr Arg Val Pro Ala Trp         355 360 365 Ala Met Arg His Ala Lys Ala Val Thr Val Gly Val Val Leu Leu Leu     370 375 380 Leu Ala Leu Thr Val Pro Leu Thr Gly Val Lys Phe Gly Gly Ile Asn 385 390 395 400 Glu Thr Tyr Leu Pro Pro Ala Asn Asp Thr Arg Val Ala Gln Glu Arg                 405 410 415 Phe Asp Glu Ala Phe Pro Ala Phe Arg Thr Glu Pro Val Lys Leu Val             420 425 430 Val Thr Gly Ala Asp Asn Asn Gln Leu Ile Asp Ile Tyr Val Gln Ala         435 440 445 Asn Glu Val Glu Gly Leu Thr Asp Arg Phe Thr Ala Gly Ala Thr Thr     450 455 460 Asp Asp Gly Thr Thr Val Leu Ser Thr Gly Ile Gln Asp Arg Ser Leu 465 470 475 480 Asn Glu Gln Val Val Glu Gln Leu Arg Ala Ile Ser Val Pro Glu Gly                 485 490 495 Val Glu Val Gln Ile Gly Gly Thr Pro Ala Met Glu Ile Glu Ser Ile             500 505 510 Glu Ala Leu Phe Glu Lys Leu Leu Trp Met Ala Leu Tyr Ile Val Leu         515 520 525 Ala Thr Phe Ile Leu Met Ala Leu Val Phe Gly Ser Val Ile Leu Pro     530 535 540 Ala Lys Ala Ile Ile Met Thr Ile Leu Gly Met Gly Ala Thr Leu Gly 545 550 555 560 Ile Leu Thr Leu Met Phe Val Asp Gly Val Gly Ala Ser Ala Leu Asn                 565 570 575 Phe Ser Pro Gly Pro Leu Met Ser Pro Val Leu Val Leu Ile Met Ala             580 585 590 Ile Ile Tyr Gly Leu Ser Thr Asp Tyr Glu Val Phe Leu Val Ser Arg         595 600 605 Met Val Glu Ala Arg Asp Lys Gly Glu Ser Thr Asp Asp Ala Ile Arg     610 615 620 Tyr Gly Thr Ala His Thr Gly Ser Ile Ile Thr Ala Ala Ala Leu Ile 625 630 635 640 Met Ile Val Val Cys Gly Ala Phe Gly Phe Ser Glu Ile Val Met Met                 645 650 655 Lys Tyr Ile Ala Phe Gly Met Ile Ala Ala Leu Ile Leu Asp Ala Thr             660 665 670 Ile Ile Arg Met Leu Leu Val Pro Arg Arg Asp Ala Pro Ala Ser Arg         675 680 685 Arg Gln Leu Val Gly Thr Arg Leu Arg     690 695 <210> 227 <211> 729 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (706) <223> RXN03042 <400> 227 atgacaccgg cgcgacgtat ggcattactg gcgtacccca atttacgatg acatctctgc 60 tcgcctcggc gacgtcctgg ttccttacgt tctgatcgtt ttg gtt cta gcg ttc 115                                             Leu Val Leu Ala Phe                                               1 5 ctc gtg ctg ttg ctc gtg ttc cgg tcc att tgg gtc cca ttg atc gcg 163 Leu Val Leu Leu Leu Val Phe Arg Ser Ile Trp Val Pro Leu Ile Ala                  10 15 20 gct ctg ggc ttt ggc ttg tca gtt ctg gct acc ttt ggt gct acc gtg 211 Ala Leu Gly Phe Gly Leu Ser Val Leu Ala Thr Phe Gly Ala Thr Val              25 30 35 gcg atc ttc caa gaa ggt gct ttc ggc atc atc gac gat cct cag cca 259 Ala Ile Phe Gln Glu Gly Ala Phe Gly Ile Ile Asp Asp Pro Gln Pro          40 45 50 ctg ctg tcc ttc ttg ccg atc atg ctc atc ggc ctg gta ttt ggt ctg 307 Leu Leu Ser Phe Leu Pro Ile Met Leu Ile Gly Leu Val Phe Gly Leu      55 60 65 gcc atg gat tac cag atc ttc ctc gtt act cgt atg cgt gag ggc ttc 355 Ala Met Asp Tyr Gln Ile Phe Leu Val Thr Arg Met Arg Glu Gly Phe  70 75 80 85 acc aag ggc aag act gcg ggc aac gca acg tcg aat ggt ttc aag cac 403 Thr Lys Gly Lys Thr Ala Gly Asn Ala Thr Ser Asn Gly Phe Lys His                  90 95 100 ggt gcc cgc gtg gtc act gct gcg gcg ctg atc atg gtg tct gtg ttc 451 Gly Ala Arg Val Val Thr Ala Ala Ala Leu Ile Met Val Ser Val Phe             105 110 115 gcg gca ttc ata gcg cag gac atg gcg ttt att aag acc atg ggc ttt 499 Ala Ala Phe Ile Ala Gln Asp Met Ala Phe Ile Lys Thr Met Gly Phe         120 125 130 gct ctg gcc gtt gct gtg ttc ttc gat gcc ttc gtt gtt cgc atg atg 547 Ala Leu Ala Val Ala Val Phe Phe Asp Ala Phe Val Val Arg Met Met     135 140 145 att atc cct gca aca atg ttc ctg ctt gat gac aag gct tgg tgg cta 595 Ile Ile Pro Ala Thr Met Phe Leu Leu Asp Asp Lys Ala Trp Trp Leu 150 155 160 165 cct aag tgg ttg gat aag att ctt ccc aac gtt gat gtt gaa ggt gag 643 Pro Lys Trp Leu Asp Lys Ile Leu Pro Asn Val Asp Val Glu Gly Glu                 170 175 180 ggt ctt agt gaa cta cat gag gct cgc acc gag gaa ctg aag gaa aat 691 Gly Leu Ser Glu Leu His Glu Ala Arg Thr Glu Glu Leu Lys Glu Asn             185 190 195 gta ggt gtc ggg gct tagagaaaca aaaaaggctg cta 729 Val Gly Val Gly Ala         200 <210> 228 <211> 202 <212> PRT <213> Corynebacterium glutamicum <400> 228 Leu Val Leu Ala Phe Leu Val Leu Leu Leu Val Phe Arg Ser Ile Trp   1 5 10 15 Val Pro Leu Ile Ala Ala Leu Gly Phe Gly Leu Ser Val Leu Ala Thr              20 25 30 Phe Gly Ala Thr Val Ala Ile Phe Gln Glu Gly Ala Phe Gly Ile Ile          35 40 45 Asp Asp Pro Gln Pro Leu Leu Ser Phe Leu Pro Ile Met Leu Ile Gly      50 55 60 Leu Val Phe Gly Leu Ala Met Asp Tyr Gln Ile Phe Leu Val Thr Arg  65 70 75 80 Met Arg Glu Gly Phe Thr Lys Gly Lys Thr Ala Gly Asn Ala Thr Ser                  85 90 95 Asn Gly Phe Lys His Gly Ala Arg Val Val Thr Ala Ala Ala Leu Ile             100 105 110 Met Val Ser Val Phe Ala Ala Phe Ile Ala Gln Asp Met Ala Phe Ile         115 120 125 Lys Thr Met Gly Phe Ala Leu Ala Val Ala Val Phe Phe Asp Ala Phe     130 135 140 Val Val Arg Met Met Ile Ile Pro Ala Thr Met Phe Leu Leu Asp Asp 145 150 155 160 Lys Ala Trp Trp Leu Pro Lys Trp Leu Asp Lys Ile Leu Pro Asn Val                 165 170 175 Asp Val Glu Gly Glu Gly Leu Ser Glu Leu His Glu Ala Arg Thr Glu             180 185 190 Glu Leu Lys Glu Asn Val Gly Val Gly Ala         195 200 <210> 229 <211> 729 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (706) <223> FRXA02893 <400> 229 atgacaccgg cgcgacgtat ggcattactg gcgtacccca atttacgatg acatctctgc 60 tcgcctcggc gacgtcctgg ttccttacgt tctgatcgtt ttg gtt cta gcg ttc 115                                             Leu Val Leu Ala Phe                                               1 5 ctc gtg ctg ttg ctc gtg ttc cgg tcc att tgg gtc cca ttg atc gcg 163 Leu Val Leu Leu Leu Val Phe Arg Ser Ile Trp Val Pro Leu Ile Ala                  10 15 20 gct ctg ggc ttt ggc ttg tca gtt ctg gct acc ttt ggt gct acc gtg 211 Ala Leu Gly Phe Gly Leu Ser Val Leu Ala Thr Phe Gly Ala Thr Val              25 30 35 gcg atc ttc caa gaa ggt gct ttc ggc atc atc gac gat cct cag cca 259 Ala Ile Phe Gln Glu Gly Ala Phe Gly Ile Ile Asp Asp Pro Gln Pro          40 45 50 ctg ctg tcc ttc ttg ccg atc atg ctc atc ggc ctg gta ttt ggt ctg 307 Leu Leu Ser Phe Leu Pro Ile Met Leu Ile Gly Leu Val Phe Gly Leu      55 60 65 gcc atg gat tac cag atc ttc ctc gtt act cgt atg cgt gag ggc ttc 355 Ala Met Asp Tyr Gln Ile Phe Leu Val Thr Arg Met Arg Glu Gly Phe  70 75 80 85 acc aag ggc aag act gcg ggc aac gca acg tcg aat ggt ttc aag cac 403 Thr Lys Gly Lys Thr Ala Gly Asn Ala Thr Ser Asn Gly Phe Lys His                  90 95 100 ggt gcc cgc gtg gtc act gct gcg gcg ctg atc atg gtg tct gtg ttc 451 Gly Ala Arg Val Val Thr Ala Ala Ala Leu Ile Met Val Ser Val Phe             105 110 115 gcg gca ttc ata gcg cag gac atg gcg ttt att aag acc atg ggc ttt 499 Ala Ala Phe Ile Ala Gln Asp Met Ala Phe Ile Lys Thr Met Gly Phe         120 125 130 gct ctg gcc gtt gct gtg ttc ttc gat gcc ttc gtt gtt cgc atg atg 547 Ala Leu Ala Val Ala Val Phe Phe Asp Ala Phe Val Val Arg Met Met     135 140 145 att atc cct gca aca atg ttc ctg ctt gat gac aag gct tgg tgg cta 595 Ile Ile Pro Ala Thr Met Phe Leu Leu Asp Asp Lys Ala Trp Trp Leu 150 155 160 165 cct aag tgg ttg gat aag att ctt ccc aac gtt gat gtt gaa ggt gag 643 Pro Lys Trp Leu Asp Lys Ile Leu Pro Asn Val Asp Val Glu Gly Glu                 170 175 180 ggt ctt agt gaa cta cat gag gct cgc acc gag gaa ctg aag gaa aat 691 Gly Leu Ser Glu Leu His Glu Ala Arg Thr Glu Glu Leu Lys Glu Asn             185 190 195 gta ggt gtc ggg gct tagagaaaca aaaaaggctg cta 729 Val Gly Val Gly Ala         200 <210> 230 <211> 202 <212> PRT <213> Corynebacterium glutamicum <400> 230 Leu Val Leu Ala Phe Leu Val Leu Leu Leu Val Phe Arg Ser Ile Trp   1 5 10 15 Val Pro Leu Ile Ala Ala Leu Gly Phe Gly Leu Ser Val Leu Ala Thr              20 25 30 Phe Gly Ala Thr Val Ala Ile Phe Gln Glu Gly Ala Phe Gly Ile Ile          35 40 45 Asp Asp Pro Gln Pro Leu Leu Ser Phe Leu Pro Ile Met Leu Ile Gly      50 55 60 Leu Val Phe Gly Leu Ala Met Asp Tyr Gln Ile Phe Leu Val Thr Arg  65 70 75 80 Met Arg Glu Gly Phe Thr Lys Gly Lys Thr Ala Gly Asn Ala Thr Ser                  85 90 95 Asn Gly Phe Lys His Gly Ala Arg Val Val Thr Ala Ala Ala Leu Ile             100 105 110 Met Val Ser Val Phe Ala Ala Phe Ile Ala Gln Asp Met Ala Phe Ile         115 120 125 Lys Thr Met Gly Phe Ala Leu Ala Val Ala Val Phe Phe Asp Ala Phe     130 135 140 Val Val Arg Met Met Ile Ile Pro Ala Thr Met Phe Leu Leu Asp Asp 145 150 155 160 Lys Ala Trp Trp Leu Pro Lys Trp Leu Asp Lys Ile Leu Pro Asn Val                 165 170 175 Asp Val Glu Gly Glu Gly Leu Ser Glu Leu His Glu Ala Arg Thr Glu             180 185 190 Glu Leu Lys Glu Asn Val Gly Val Gly Ala         195 200 <210> 231 <211> 1605 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1582) <223> RXA01616 <400> 231 cctacgattt ccgacgtctt aaatcgccac acagcgccgt ggtctaaaac accaacaaaa 60 gagttgtaac tgtaccgacc attcgttaca gttacgatcc atg act tca gaa acc 115                                             Met Thr Ser Glu Thr                                               1 5 tta cag gcg caa gcg cct acg aaa acc caa cgt tgg gct ttc ctc gcc 163 Leu Gln Ala Gln Ala Pro Thr Lys Thr Gln Arg Trp Ala Phe Leu Ala                  10 15 20 gtt atc agc ggt ggt ctc ttt ctg atc ggt gta gac aac tcg att ctc 211 Val Ile Ser Gly Gly Leu Phe Leu Ile Gly Val Asp Asn Ser Ile Leu              25 30 35 tac acc gca ctc cct ctg ctg cgt gaa cag ctc gca gcc tcc gaa acc 259 Tyr Thr Ala Leu Pro Leu Leu Arg Glu Gln Leu Ala Ala Ser Glu Thr          40 45 50 caa gcg ttg tgg atc atc aac gca tat ccc ctg ctc atg gcg ggc ctt 307 Gln Ala Leu Trp Ile Ile Asn Ala Tyr Pro Leu Leu Met Ala Gly Leu      55 60 65 cgt ttg ggt gcc ggc act ttg ggt gac aaa aac ggc cac cgc cgg atg 355 Arg Leu Gly Ala Gly Thr Leu Gly Asp Lys Asn Gly His Arg Arg Met  70 75 80 85 ttc ctc atg ggc ttg agc att ttc gga atc gct tca ctt ggt gct gcg 403 Phe Leu Met Gly Leu Ser Ile Phe Gly Ile Ala Ser Leu Gly Ala Ala                  90 95 100 ttt gct cca act gcg tgg gct ctt gtt gct gcg aga gct ttc ctt ggc 451 Phe Ala Pro Thr Ala Trp Ala Leu Val Ala Ala Arg Ala Phe Leu Gly             105 110 115 atc ggt gcg gca acg atg atg cct gca acc ttg gct ctg atc cgc att 499 Ile Gly Ala Ala Thr Met Met Pro Ala Thr Leu Ala Leu Ile Arg Ile         120 125 130 acg ttt gag gat gag cgt gag cgc aac act gca att ggt att tgg ggt 547 Thr Phe Glu Asp Glu Arg Glu Arg Asn Thr Ala Ile Gly Ile Trp Gly     135 140 145 tcc gtg gca att ctt ggc gct gcg gca ggc ccg atc att ggt ggt gcg 595 Ser Val Ala Ile Leu Gly Ala Ala Ala Gly Pro Ile Ile Gly Gly Ala 150 155 160 165 ctg ttg gaa ttc ttc tgg tgg ggt tcg gtt ttc ctc att aac gtt ccg 643 Leu Leu Glu Phe Phe Trp Trp Gly Ser Val Phe Leu Ile Asn Val Pro                 170 175 180 gtg gct gtt atc gcg ttg atc gct acg ctt ttt gtg gcg ccg gcc aat 691 Val Ala Val Ile Ala Leu Ile Ala Thr Leu Phe Val Ala Pro Ala Asn             185 190 195 atc gcg aat ccg tct aag cat tgg gat ttc ttg tcg tcg ttc tat gcg 739 Ile Ala Asn Pro Ser Lys His Trp Asp Phe Leu Ser Ser Phe Tyr Ala         200 205 210 ctg ctc aca ctt gct ggg ttg atc atc acg atc aag gaa tct gtg aat 787 Leu Leu Thr Leu Ala Gly Leu Ile Ile Thr Ile Lys Glu Ser Val Asn     215 220 225 act gca cgc cat atg cct ctt ctt ttg ggt gca gtc atc atg ttg atc 835 Thr Ala Arg His Met Pro Leu Leu Leu Gly Ala Val Ile Met Leu Ile 230 235 240 245 att ggt gcg gtg ttg ttt agc agt cgt cag aag aag atc gag gag cca 883 Ile Gly Ala Val Leu Phe Ser Ser Arg Gln Lys Lys Ile Glu Glu Pro                 250 255 260 ctt cta gat ctg tcg ttg ttc cgt aat cgc ctt ttc tta ggc ggt gtg 931 Leu Leu Asp Leu Ser Leu Phe Arg Asn Arg Leu Phe Leu Gly Gly Val             265 270 275 gtt gct gcg ggc atg gcg atg ttt act gtg tcc ggt ttg gaa atg act 979 Val Ala Ala Gly Met Ala Met Phe Thr Val Ser Gly Leu Glu Met Thr         280 285 290 acc tcg cag cgt ttc cag ttg tct gtg ggt ttc act cca ctt gag gct 1027 Thr Ser Gln Arg Phe Gln Leu Ser Val Gly Phe Thr Pro Leu Glu Ala     295 300 305 ggt ttg ctc atg atc cca gct gca ttg ggt agc ttc ccg atg tct att 1075 Gly Leu Leu Met Ile Pro Ala Ala Leu Gly Ser Phe Pro Met Ser Ile 310 315 320 325 atc ggt ggt gca aac ctg cat cgt tgg ggc ttc aaa ccg ctg atc agt 1123 Ile Gly Gly Ala Asn Leu His Arg Trp Gly Phe Lys Pro Leu Ile Ser                 330 335 340 ggt ggt ttt gct gcc act gcc gtt ggc atc gcc ctg tgt att tgg ggc 1171 Gly Gly Phe Ala Ala Thr Ala Val Gly Ile Ala Leu Cys Ile Trp Gly             345 350 355 gcg act cat act gat ggt ttg ccg ttt ttc atc gcg ggt cta ttc ttc 1219 Ala Thr His Thr Asp Gly Leu Pro Phe Phe Ile Ala Gly Leu Phe Phe         360 365 370 atg ggc gcg ggt gct ggt tcg gta atg tct gtg tct tcc act gcg att 1267 Met Gly Ala Gly Ala Gly Ser Val Met Ser Val Ser Ser Thr Ala Ile     375 380 385 atc ggt tcc gcg ccg gtg cgt aag gct ggc atg gcg tcg tcg atc gaa 1315 Ile Gly Ser Ala Pro Val Arg Lys Ala Gly Met Ala Ser Ser Ile Glu 390 395 400 405 gag gtc tct tat gag ttc ggc acg ctg ttg tct gtc gcg att ttg ggt 1363 Glu Val Ser Tyr Glu Phe Gly Thr Leu Leu Ser Val Ala Ile Leu Gly                 410 415 420 agc ttg ttc cca ttc ttc tac tcg ctg cat gcc ccg gca gag gtt gcg 1411 Ser Leu Phe Pro Phe Phe Tyr Ser Leu His Ala Pro Ala Glu Val Ala             425 430 435 gat aac ttc tcg gcg ggt gtt cac cac gcg att gat ggc gat gcg gcg 1459 Asp Asn Phe Ser Ala Gly Val His His Ala Ile Asp Gly Asp Ala Ala         440 445 450 cgt gca tct ttg gac acc gca tac att aac gtg ttg atc att gcc cta 1507 Arg Ala Ser Leu Asp Thr Ala Tyr Ile Asn Val Leu Ile Ile Ala Leu     455 460 465 gta tgc gca gta gcg gct gct ctg atc agc agt tac ctt ttc cgc gga 1555 Val Cys Ala Val Ala Ala Ala Leu Ile Ser Ser Tyr Leu Phe Arg Gly 470 475 480 485 aat ccg aag gga gcc aat aat gcg cac tagtaaaaaa gagatgattc 1602 Asn Pro Lys Gly Ala Asn Asn Ala His                 490 tgc 1605 <210> 232 <211> 494 <212> PRT <213> Corynebacterium glutamicum <400> 232 Met Thr Ser Glu Thr Leu Gln Ala Gln Ala Pro Thr Lys Thr Gln Arg   1 5 10 15 Trp Ala Phe Leu Ala Val Ile Ser Gly Gly Leu Phe Leu Ile Gly Val              20 25 30 Asp Asn Ser Ile Leu Tyr Thr Ala Leu Pro Leu Leu Arg Glu Gln Leu          35 40 45 Ala Ala Ser Glu Thr Gln Ala Leu Trp Ile Ile Asn Ala Tyr Pro Leu      50 55 60 Leu Met Ala Gly Leu Arg Leu Gly Ala Gly Thr Leu Gly Asp Lys Asn  65 70 75 80 Gly His Arg Arg Met Phe Leu Met Gly Leu Ser Ile Phe Gly Ile Ala                  85 90 95 Ser Leu Gly Ala Ala Phe Ala Pro Thr Ala Trp Ala Leu Val Ala Ala             100 105 110 Arg Ala Phe Leu Gly Ile Gly Ala Ala Thr Met Met Pro Ala Thr Leu         115 120 125 Ala Leu Ile Arg Ile Thr Phe Glu Asp Glu Arg Glu Arg Asn Thr Ala     130 135 140 Ile Gly Ile Trp Gly Ser Val Ala Ile Leu Gly Ala Ala Ala Gly Pro 145 150 155 160 Ile Ile Gly Gly Ala Leu Leu Glu Phe Phe Trp Trp Gly Ser Val Phe                 165 170 175 Leu Ile Asn Val Pro Val Ala Val Ile Ala Leu Ile Ala Thr Leu Phe             180 185 190 Val Ala Pro Ala Asn Ile Ala Asn Pro Ser Lys His Trp Asp Phe Leu         195 200 205 Ser Ser Phe Tyr Ala Leu Leu Thr Leu Ala Gly Leu Ile Ile Thr Ile     210 215 220 Lys Glu Ser Val Asn Thr Ala Arg His Met Pro Leu Leu Leu Gly Ala 225 230 235 240 Val Ile Met Leu Ile Ile Gly Ala Val Leu Phe Ser Ser Arg Gln Lys                 245 250 255 Lys Ile Glu Glu Pro Leu Leu Asp Leu Ser Leu Phe Arg Asn Arg Leu             260 265 270 Phe Leu Gly Gly Val Val Ala Ala Gly Met Ala Met Phe Thr Val Ser         275 280 285 Gly Leu Glu Met Thr Thr Ser Gln Arg Phe Gln Leu Ser Val Gly Phe     290 295 300 Thr Pro Leu Glu Ala Gly Leu Leu Met Ile Pro Ala Ala Leu Gly Ser 305 310 315 320 Phe Pro Met Ser Ile Ile Gly Gly Ala Asn Leu His Arg Trp Gly Phe                 325 330 335 Lys Pro Leu Ile Ser Gly Gly Phe Ala Ala Thr Ala Val Gly Ile Ala             340 345 350 Leu Cys Ile Trp Gly Ala Thr His Thr Asp Gly Leu Pro Phe Phe Ile         355 360 365 Ala Gly Leu Phe Phe Met Gly Ala Gly Ala Gly Ser Val Met Ser Val     370 375 380 Ser Ser Thr Ala Ile Gly Ser Ala Pro Val Arg Lys Ala Gly Met 385 390 395 400 Ala Ser Ser Ile Glu Glu Val Ser Tyr Glu Phe Gly Thr Leu Leu Ser                 405 410 415 Val Ala Ile Leu Gly Ser Leu Phe Pro Phe Phe Tyr Ser Leu His Ala             420 425 430 Pro Ala Glu Val Ala Asp Asn Phe Ser Ala Gly Val His His Ala Ile         435 440 445 Asp Gly Asp Ala Ala Arg Ala Ser Leu Asp Thr Ala Tyr Ile Asn Val     450 455 460 Leu Ile Ile Ala Leu Val Cys Ala Val Ala Ala Ala Leu Ile Ser Ser 465 470 475 480 Tyr Leu Phe Arg Gly Asn Pro Lys Gly Ala Asn Asn Ala His                 485 490 <210> 233 <211> 1500 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (1477) <223> RXA01666 <400> 233 cgacgcgccc ctccaccttt tcagtagcgt cacgggcgcc aatcctgtat ttttagcagc 60 agtttgaggg tttttgctcc ccatctttag gagacacccc gtg tcc acg ttt cat 115                                             Val Ser Thr Phe His                                               1 5 aaa gtt ttg atc aac acc atg atc tcc aac gtc acc act gga ttt ctg 163 Lys Val Leu Ile Asn Thr Met Ile Ser Asn Val Thr Thr Gly Phe Leu                  10 15 20 ttc ttt gcc gtg gtg ttt tgg atg tat ctt tcc act ggc aac gtc gca 211 Phe Phe Ala Val Val Phe Trp Met Tyr Leu Ser Thr Gly Asn Val Ala              25 30 35 ctg acc ggc atc gtc agt gga att tac atg ggt ttg atc gcc gtt tgt 259 Leu Thr Gly Ile Val Ser Gly Ile Tyr Met Gly Leu Ile Ala Val Cys          40 45 50 tcc atc ttt ttc gga acc gtt gtt gat cac aat cgc aag aag tcc gtc 307 Ser Ile Phe Phe Gly Thr Val Val Asp His Asn Arg Lys Lys Ser Val      55 60 65 atg ctg ttt tcc agc gtc acc aca ctc gtg ttt tat tgt ctc agt gcc 355 Met Leu Phe Ser Ser Val Thr Thr Leu Val Phe Tyr Cys Leu Ser Ala  70 75 80 85 ctg gtg tgg gtg ttt tgg ctg gag gaa gac ggc ctg agc atc gga aat 403 Leu Val Trp Val Phe Trp Leu Glu Glu Asp Gly Leu Ser Ile Gly Asn                  90 95 100 acc gcc ctg tgg gtg ttc gtt tct ttc atc ctc atc gga tca atc gtg 451 Thr Ala Leu Trp Val Phe Val Ser Phe Ile Leu Ile Gly Ser Ile Val             105 110 115 gaa cac atg cgc aac atc gca ctg tcc acc gtg gtc acg ctg ttg gtt 499 Glu His Met Arg Asn Ile Ala Leu Ser Thr Val Val Thr Leu Leu Val         120 125 130 cct gaa gct gaa cgc gac aaa gca aac ggc ctg gta gga gcc gtg caa 547 Pro Glu Ala Glu Arg Asp Lys Ala Asn Gly Leu Val Gly Ala Val Gln     135 140 145 ggt gtt gga ttt tta gtc acc agc gtc att gct ggt tcc gcc atc ggg 595 Gly Val Gly Phe Leu Val Thr Ser Val Ile Ala Gly Ser Ala Ile Gly 150 155 160 165 ttc ttg ggc atg gaa atc acc ctg tgg atc tgc ctt ggg ctc tca ctt 643 Phe Leu Gly Met Glu Ile Thr Leu Trp Ile Cys Leu Gly Leu Ser Leu                 170 175 180 gtc gcg ctg ctg cac ctg ctg ccg att cgc gtc gac gaa ccg gaa atc 691 Val Ala Leu Leu His Leu Leu Pro Ile Arg Val Asp Glu Pro Glu Ile             185 190 195 atc acc caa gaa gac gca cag cct act gtt tct gac gat tca gtt ccc 739 Ile Thr Gln Glu Asp Ala Gln Pro Thr Val Ser Asp Asp Ser Val Pro         200 205 210 aca cct acc tcc gat ttg gcg atc gtg tcc aaa ggc atc gac cta aaa 787 Thr Pro Thr Ser Asp Leu Ala Ile Val Ser Lys Gly Ile Asp Leu Lys     215 220 225 gga tca atg aaa atc atc ctg agt gtt ccg gga ctg ctc gcg ctt gtg 835 Gly Ser Met Lys Ile Ile Leu Ser Val Pro Gly Leu Leu Ala Leu Val 230 235 240 245 ttg ttt gcg tcc ttc aac aac ctc atc ggc ggc gtg tac tcc gca ctc 883 Leu Phe Ala Ser Phe Asn Asn Leu Ile Gly Gly Val Tyr Ser Ala Leu                 250 255 260 atg gac cct tac ggc ctg gaa ctt ttc agc cca cag ctg tgg ggg cta 931 Met Asp Pro Tyr Gly Leu Glu Leu Phe Ser Pro Gln Leu Trp Gly Leu             265 270 275 ctg ctt gga ctc acc agc ctc ggc ttc atc gtt ggt ggt gct gtg atc 979 Leu Leu Gly Leu Thr Ser Leu Gly Phe Ile Val Gly Gly Ala Val Ile         280 285 290 tcc aaa act ggc ttg ggc aaa aac cct gtg cgc acc ttg ctg ctg gtt 1027 Ser Lys Thr Gly Leu Gly Lys Asn Pro Val Arg Thr Leu Leu Leu Val     295 300 305 aat gtt ggt gtg gct ttt gtt ggc atg tta ttt gcc att cgc gaa tgg 1075 Asn Val Gly Val Ala Phe Val Gly Met Leu Phe Ala Ile Arg Glu Trp 310 315 320 325 tgg tgg ctc tac atc ctg ggc att ttc atc ttc atg gct atc acc cca 1123 Trp Trp Leu Tyr Ile Leu Gly Ile Phe Ile Phe Met Ala Ile Thr Pro                 330 335 340 gct gcc gaa gcc gca gaa caa acc atc ctt caa cga gtc gtc cca ttc 1171 Ala Ala Glu Ala Ala Glu Gln Thr Ile Leu Gln Arg Val Val Pro Phe             345 350 355 cgc caa caa ggc cgc gta ttt gga cta gcc atg gca gtg gaa atg gca 1219 Arg Gln Gln Gly Arg Val Phe Gly Leu Ala Met Ala Val Glu Met Ala         360 365 370 gcc aac ccg ctc tcc aca gtg atc gtg gcg att ttg gcc gaa gcc tac 1267 Ala Asn Pro Leu Ser Thr Val Ile Val Ala Ile Leu Ala Glu Ala Tyr     375 380 385 ctc att cca tgg atg gct ggc ccc ggc gcg gac acc atc tgg ggc gtg 1315 Leu Ile Pro Trp Met Ala Gly Pro Gly Ala Asp Thr Ile Trp Gly Val 390 395 400 405 atc ctc ggc gag ggt aaa gct cgc ggc atg gca ctg atg ttc ctc gca 1363 Ile Leu Gly Glu Gly Lys Ala Arg Gly Met Ala Leu Met Phe Leu Ala                 410 415 420 tca ggt gcc atc atg ttg gtt gtc gtg ctg ttg gca ttc atg tcg agg 1411 Ser Gly Ala Ile Met Leu Val Val Val Leu Leu Ala Phe Met Ser Arg             425 430 435 tcc tac cgg aaa ctc agc cag tac tac gcc acc acc agc caa gac att 1459 Ser Tyr Arg Lys Leu Ser Gln Tyr Tyr Ala Thr Thr Ser Gln Asp Ile         440 445 450 gcg gga gct gct gag aag taagtgctct agaccgttgt ttg 1500 Ala Gly Ala Ala Glu Lys     455 <210> 234 <211> 459 <212> PRT <213> Corynebacterium glutamicum <400> 234 Val Ser Thr Phe His Lys Val Leu Ile Asn Thr Met Ile Ser Asn Val   1 5 10 15 Thr Thr Gly Phe Leu Phe Phe Ala Val Val Phe Trp Met Tyr Leu Ser              20 25 30 Thr Gly Asn Val Ala Leu Thr Gly Ile Val Ser Gly Ile Tyr Met Gly          35 40 45 Leu Ile Ala Val Cys Ser Ile Phe Ghe Thr Val Val Asp His Asn      50 55 60 Arg Lys Lys Ser Val Met Leu Phe Ser Ser Val Thr Thr Leu Val Phe  65 70 75 80 Tyr Cys Leu Ser Ala Leu Val Trp Val Phe Trp Leu Glu Glu Asp Gly                  85 90 95 Leu Ser Ile Gly Asn Thr Ala Leu Trp Val Phe Val Ser Phe Ile Leu             100 105 110 Ile Gly Ser Ile Val Glu His Met Arg Asn Ile Ala Leu Ser Thr Val         115 120 125 Val Thr Leu Leu Val Pro Glu Ala Glu Arg Asp Lys Ala Asn Gly Leu     130 135 140 Val Gly Ala Val Gln Gly Val Gly Phe Leu Val Thr Ser Val Ile Ala 145 150 155 160 Gly Ser Ala Ile Gly Phe Leu Gly Met Glu Ile Thr Leu Trp Ile Cys                 165 170 175 Leu Gly Leu Ser Leu Val Ala Leu Leu His Leu Leu Pro Ile Arg Val             180 185 190 Asp Glu Pro Glu Ile Ile Thr Gln Glu Asp Ala Gln Pro Thr Val Ser         195 200 205 Asp Asp Ser Val Pro Thr Pro Thr Ser Asp Leu Ala Ile Val Ser Lys     210 215 220 Gly Ile Asp Leu Lys Gly Ser Met Lys Ile Ile Leu Ser Val Pro Gly 225 230 235 240 Leu Leu Ala Leu Val Leu Phe Ala Ser Phe Asn Asn Leu Ile Gly Gly                 245 250 255 Val Tyr Ser Ala Leu Met Asp Pro Tyr Gly Leu Glu Leu Phe Ser Pro             260 265 270 Gln Leu Trp Gly Leu Leu Leu Gly Leu Thr Ser Leu Gly Phe Ile Val         275 280 285 Gly Gly Ala Val Ile Ser Lys Thr Gly Leu Gly Lys Asn Pro Val Arg     290 295 300 Thr Leu Leu Leu Val Asn Val Gly Val Ala Phe Val Gly Met Leu Phe 305 310 315 320 Ala Ile Arg Glu Trp Trp Trp Leu Tyr Ile Leu Gly Ile Phe Ile Phe                 325 330 335 Met Ala Ile Thr Pro Ala Ala Glu Ala Ala Glu Gln Thr Ile Leu Gln             340 345 350 Arg Val Val Pro Phe Arg Gln Gln Gly Arg Val Phe Gly Leu Ala Met         355 360 365 Ala Val Glu Met Ala Ala Asn Pro Leu Ser Thr Val Ile Val Ala Ile     370 375 380 Leu Ala Glu Ala Tyr Leu Ile Pro Trp Met Ala Gly Pro Gly Ala Asp 385 390 395 400 Thr Ile Trp Gly Val Ile Leu Gly Glu Gly Lys Ala Arg Gly Met Ala                 405 410 415 Leu Met Phe Leu Ala Ser Gly Ala Ile Met Leu Val Val Val Leu Leu             420 425 430 Ala Phe Met Ser Arg Ser Tyr Arg Lys Leu Ser Gln Tyr Tyr Ala Thr         435 440 445 Thr Ser Gln Asp Ile Ala Gly Ala Ala Glu Lys     450 455 <210> 235 <211> 1521 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1498) <223> RXA00062 <400> 235 cttcaacata ggcgttgggg ctgactttta aacaggtacc agtagtaccg gcataagcga 60 tcactgttgc gttttcttgc tgccatcaaa aattagtcac atg att tta agc atc 115                                             Met Ile Leu Ser Ile                                               1 5 gtc ctt ttg ggc tac ttc atg att ctg ctt gac acc tcc atc gtc att 163 Val Leu Leu Gly Tyr Phe Met Ile Leu Leu Asp Thr Ser Ile Val Ile                  10 15 20 acg ggt cta cct gcc atc ggc agt gaa ctt ggc atc gat ccc gtg cac 211 Thr Gly Leu Pro Ala Ile Gly Ser Glu Leu Gly Ile Asp Pro Val His              25 30 35 ctg tca tgg gtg cag agt tcc tac aca tta gtc ttc ggc gca ctt ctt 259 Leu Ser Trp Val Gln Ser Ser Tyr Thr Leu Val Phe Gly Ala Leu Leu          40 45 50 ctg ctg gga gct cgt gcc ggt gat atc ttc ggc cga aag aaa gtg ctc 307 Leu Leu Gly Ala Arg Ala Gly Asp Ile Phe Gly Arg Lys Lys Val Leu      55 60 65 tac att ggt ctc gcg ttg ttt gcg gct tca tcg ttg gca att gcg ctt 355 Tyr Ile Gly Leu Ala Leu Phe Ala Ala Ser Ser Leu Ala Ile Ala Leu  70 75 80 85 tct cca aat gct gcg gtc ctc att gga gca cgc gta gtt caa ggc gcg 403 Ser Pro Asn Ala Ala Val Leu Ile Gly Ala Arg Val Val Gln Gly Ala                  90 95 100 gga gct gcg att atc gct cca gcg aca ctt gcg ttg att act gag ttc 451 Gly Ala Ala Ile Ile Ala Pro Ala Thr Leu Ala Leu Ile Thr Glu Phe             105 110 115 ttc ccc gaa ggc cca gct cgc ctt cgt gct acc tct gct tat ggt gct 499 Phe Pro Glu Gly Pro Ala Arg Leu Arg Ala Thr Ser Ala Tyr Gly Ala         120 125 130 gtt gcc ggc atc ggt gtg gca gca ggc cta gtg atc ggc ggc gta ttt 547 Val Ala Gly Ile Gly Val Ala Ala Gly Leu Val Ile Gly Gly Val Phe     135 140 145 gct gat ctt ttg tcg tgg cgc atc ggc ttc ttt atc aac gtc ccc atc 595 Ala Asp Leu Leu Ser Trp Arg Ile Gly Phe Phe Ile Asn Val Pro Ile 150 155 160 165 gcc gca gtg ttg gcc tac ata gtg cac aaa gcc att ccc gca acc ttc 643 Ala Ala Val Leu Ala Tyr Ile Val His Lys Ala Ile Pro Ala Thr Phe                 170 175 180 agc agg cct gga tca ctc gac atc ttc gga gca att acc tcc acg gca 691 Ser Arg Pro Gly Ser Leu Asp Ile Phe Gly Ala Ile Thr Ser Thr Ala             185 190 195 ggt atc gcc gcg gtg ctc tac gca att gtc cgc agc gcc gat tac agc 739 Gly Ile Ala Ala Val Leu Tyr Ala Ile Val Arg Ser Ala Asp Tyr Ser         200 205 210 tgg aca gat ccg ttt gtg ttg att tcc ctc gtg ctg ggc atc gca gtg 787 Trp Thr Asp Pro Phe Val Leu Ile Ser Leu Val Leu Gly Ile Ala Val     215 220 225 ttc atc tgg ttc ctg cgc cat gaa tcc tca gcc aaa gaa cca ctt ctg 835 Phe Ile Trp Phe Leu Arg His Glu Ser Ser Ala Lys Glu Pro Leu Leu 230 235 240 245 ccc ctg ggg ctc ttt aaa aac cgc agg cga aac acc atc ttg gcc agc 883 Pro Leu Gly Leu Phe Lys Asn Arg Arg Arg Asn Thr Ile Leu Ala Ser                 250 255 260 cgc ttt ctt ctg gtt ggc tcc gtg atg tca ttc ttc ttc ttt gcc acc 931 Arg Phe Leu Leu Val Gly Ser Val Met Ser Phe Phe Phe Phe Ala Thr             265 270 275 cag ctg ttc cag gac acc atg gga atg aat gct ctc cag gca ggc ctt 979 Gln Leu Phe Gln Asp Thr Met Gly Met Asn Ala Leu Gln Ala Gly Leu         280 285 290 gcg ttc atg ccg cta tct ctg ctg cag ttt gcc agc gcc gcg atg gtg 1027 Ala Phe Met Pro Leu Ser Leu Leu Gln Phe Ala Ser Ala Ala Met Val     295 300 305 cca cgg ctt tcc cga gca ggc gta tct gat tcc atg ctc acc gtc atc 1075 Pro Arg Leu Ser Arg Ala Gly Val Ser Asp Ser Met Leu Thr Val Ile 310 315 320 325 ggt ttc gcc atc atg gtc atc ggc atg gca ggc ctc gca ttt gta cca 1123 Gly Phe Ala Ile Met Val Ile Gly Met Ala Gly Leu Ala Phe Val Pro                 330 335 340 aac acg atg atc gcg ctg atc cta cca ata gtt ttg gtg gga ttt ggc 1171 Asn Thr Met Ile Ala Leu Ile Leu Pro Ile Val Leu Val Gly Phe Gly             345 350 355 caa ggc ttc gct ttc gga cca atg aca gct ctg gca gtt caa ggt gca 1219 Gln Gly Phe Ala Phe Gly Pro Met Thr Ala Leu Ala Val Gln Gly Ala         360 365 370 ccg aag gac caa tcc ggc gcc gtt tct ggc ctg gtg aat tcc ctt cac 1267 Pro Lys Asp Gln Ser Gly Ala Val Ser Gly Leu Val Asn Ser Leu His     375 380 385 caa atc ggc ggc acc ttc ggt ttg ggt gtg ttc tcc tcc ttg gct gtc 1315 Gln Ile Gly Gly Thr Phe Gly Leu Gly Val Phe Ser Ser Leu Ala Val 390 395 400 405 gct gtc atc gga cat gat gca aca tca gag atg atc agc gac cgc gca 1363 Ala Val Ile Gly His Asp Ala Thr Ser Glu Met Ile Ser Asp Arg Ala                 410 415 420 cac ttc gga ttc ttg ctc tcc acc gtg acg ctg acg ctg gcc acc atc 1411 His Phe Gly Phe Leu Leu Ser Thr Val Thr Leu Thr Leu Ala Thr Ile             425 430 435 ttt gcg gtc aca ctg ctg aag cgc cac gaa acc cga aag agt agc gag 1459 Phe Ala Val Thr Leu Leu Lys Arg His Glu Thr Arg Lys Ser Ser Glu         440 445 450 cgc cca acc cag ctc gtc gac gaa aag gca gtt acc tct tagtgcgctg 1508 Arg Pro Thr Gln Leu Val Asp Glu Lys Ala Val Thr Ser     455 460 465 cagcatccca gtt 1521 <210> 236 <211> 466 <212> PRT <213> Corynebacterium glutamicum <400> 236 Met Ile Leu Ser Ile Val Leu Leu Gly Tyr Phe Met Ile Leu Leu Asp   1 5 10 15 Thr Ser Ile Val Ile Thr Gly Leu Pro Ala Ile Gly Ser Glu Leu Gly              20 25 30 Ile Asp Pro Val His Leu Ser Trp Val Gln Ser Ser Tyr Thr Leu Val          35 40 45 Phe Gly Ala Leu Leu Leu Leu Gly Ala Arg Ala Gly Asp Ile Phe Gly      50 55 60 Arg Lys Lys Val Leu Tyr Ile Gly Leu Ala Leu Phe Ala Ala Ser Ser  65 70 75 80 Leu Ala Ile Ala Leu Ser Pro Asn Ala Ala Val Leu Ile Gly Ala Arg                  85 90 95 Val Val Gln Gly Ala Gly Ala Ala Ile Ile Ala Pro Ala Thr Leu Ala             100 105 110 Leu Ile Thr Glu Phe Phe Pro Glu Gly Pro Ala Arg Leu Arg Ala Thr         115 120 125 Ser Ala Tyr Gly Ala Val Ala Gly Ile Gly Val Ala Ala Gly Leu Val     130 135 140 Ile Gly Gly Val Phe Ala Asp Leu Leu Ser Trp Arg Ile Gly Phe Phe 145 150 155 160 Ile Asn Val Pro Ile Ala Ala Val Leu Ala Tyr Ile Val His Lys Ala                 165 170 175 Ile Pro Ala Thr Phe Ser Arg Pro Gly Ser Leu Asp Ile Phe Gly Ala             180 185 190 Ile Thr Ser Thr Ala Gly Ile Ala Ala Val Leu Tyr Ala Ile Val Arg         195 200 205 Ser Ala Asp Tyr Ser Trp Thr Asp Pro Phe Val Leu Ile Ser Leu Val     210 215 220 Leu Gly Ile Ala Val Phe Ile Trp Phe Leu Arg His Glu Ser Ser Ala 225 230 235 240 Lys Glu Pro Leu Leu Pro Leu Gly Leu Phe Lys Asn Arg Arg Arg Asn                 245 250 255 Thr Ile Leu Ala Ser Arg Phe Leu Leu Val Gly Ser Val Met Ser Phe             260 265 270 Phe Phe Phe Ala Thr Gln Leu Phe Gln Asp Thr Met Gly Met Asn Ala         275 280 285 Leu Gln Ala Gly Leu Ala Phe Met Pro Leu Ser Leu Leu Gln Phe Ala     290 295 300 Ser Ala Ala Met Val Pro Arg Leu Ser Arg Ala Gly Val Ser Asp Ser 305 310 315 320 Met Leu Thr Val Ile Gly Phe Ala Ile Met Val Ile Gly Met Ala Gly                 325 330 335 Leu Ala Phe Val Pro Asn Thr Met Ile Ala Leu Ile Leu Pro Ile Val             340 345 350 Leu Val Gly Phe Gly Gln Gly Phe Ala Phe Gly Pro Met Thr Ala Leu         355 360 365 Ala Val Gln Gly Ala Pro Lys Asp Gln Ser Gly Ala Val Ser Gly Leu     370 375 380 Val Asn Ser Leu His Gln Ile Gly Gly Thr Phe Gly Leu Gly Val Phe 385 390 395 400 Ser Ser Leu Ala Val Ala Val Ile Gly His Asp Ala Thr Ser Glu Met                 405 410 415 Ile Ser Asp Arg Ala His Phe Gly Phe Leu Leu Ser Thr Val Thr Leu             420 425 430 Thr Leu Ala Thr Ile Phe Ala Val Thr Leu Leu Lys Arg His Glu Thr         435 440 445 Arg Lys Ser Ser Glu Arg Pro Thr Gln Leu Val Asp Glu Lys Ala Val     450 455 460 Thr ser 465 <210> 237 <211> 1584 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1561) <223> RXA00215 <400> 237 cagtgcaaac tgaccccgca tcctaaaccg cgccagattt ctacctcaaa gaattgaagg 60 ccttttccag gcgccctcgt gcgtgaaaga ataactcaac gtg tct gac aaa aag 115                                             Val Ser Asp Lys Lys                                               1 5 cag gat cta aca tcc tcc gca gca ggt agt gct gca ccc caa acc aag 163 Gln Asp Leu Thr Ser Ser Ala Ala Gly Ser Ala Ala Pro Gln Thr Lys                  10 15 20 gcc tac ccc gcc atg ccc ttg cct gaa aag caa gct tgg cca gct cta 211 Ala Tyr Pro Ala Met Pro Leu Pro Glu Lys Gln Ala Trp Pro Ala Leu              25 30 35 att gcc ttg tgc att ggg ttt ttc atg atc ctg ttg gat caa acc atc 259 Ile Ala Leu Cys Ile Gly Phe Phe Met Ile Leu Leu Asp Gln Thr Ile          40 45 50 gtg gcc gtc tct acc cca gcg tta cag gca gac atg ggc gcg tcc tac 307 Val Ala Val Ser Thr Pro Ala Leu Gln Ala Asp Met Gly Ala Ser Tyr      55 60 65 aac gag gtc atc tgg gta acc tcg gtg tat ctc ctc act ttc gcg gtg 355 Asn Glu Val Ile Trp Val Thr Ser Val Tyr Leu Leu Thr Phe Ala Val  70 75 80 85 cca ctg ctt gtt act ggc cgt ttg ggc gac aag tac ggt ccg aaa aat 403 Pro Leu Leu Val Thr Gly Arg Leu Gly Asp Lys Tyr Gly Pro Lys Asn                  90 95 100 gtc tat gtc gca ggc atg gtt atc ttc aca gtg agc tct ttg gcc tgt 451 Val Tyr Val Ala Gly Met Val Ile Phe Thr Val Ser Ser Leu Ala Cys             105 110 115 ggt ttg gcc cca gac atg ttc acg ttg att atc gct cgt ggc gtt caa 499 Gly Leu Ala Pro Asp Met Phe Thr Leu Ile Ile Ala Arg Gly Val Gln         120 125 130 ggt ttg ggc gca gcc ctt ttg act cca caa acc atg gca aca atc aac 547 Gly Leu Gly Ala Ala Leu Leu Thr Pro Gln Thr Met Ala Thr Ile Asn     135 140 145 cgc atc ttt gct ttt gag cgc cgc ggt gca gct ctt gga gtg tgg ggt 595 Arg Ile Phe Ala Phe Glu Arg Arg Gly Ala Ala Leu Gly Val Trp Gly 150 155 160 165 tct aca gct ggc ctt gca tcc cta gca gga ccg atc ctg ggt ggt gtc 643 Ser Thr Ala Gly Leu Ala Ser Leu Ala Gly Pro Ile Leu Gly Gly Val                 170 175 180 atc acc gaa aac tgg ggt tgg caa tgg gtc ttc tac atc aac gtg ccc 691 Ile Thr Glu Asn Trp Gly Trp Gln Trp Val Phe Tyr Ile Asn Val Pro             185 190 195 atc ggc gtg atc tcg gtg atc gca gta atg aag tac gtt cct gaa ttc 739 Ile Gly Val Ile Ser Val Ile Ala Val Met Lys Tyr Val Pro Glu Phe         200 205 210 cca ccg ctg acc cga ccg ctt gat ccg ctt tct atc gtg ttg tcc atc 787 Pro Pro Leu Thr Arg Pro Leu Asp Pro Leu Ser Ile Val Leu Ser Ile     215 220 225 gtg gcc gtg ttc ttc ctg gtg ttt gct ttc cag gaa ggc gaa ggc gct 835 Val Ala Val Phe Phe Leu Val Phe Ala Phe Gln Glu Gly Glu Gly Ala 230 235 240 245 ggc tgg gcg gca tgg gtg tgg atc atg atc gta gcc gcc ttt gcg ctc 883 Gly Trp Ala Ala Trp Val Trp Ile Met Ile Val Ala Ala Phe Ala Leu                 250 255 260 ttt gcg tgg ttt atc tac caa caa agc agg gcc gag aaa tcc gga aac 931 Phe Ala Trp Phe Ile Tyr Gln Gln Ser Arg Ala Glu Lys Ser Gly Asn             265 270 275 gat cct ctc gtc cca ctg gag att ttc aag ttt aga aac ttc agc ctc 979 Asp Pro Leu Val Pro Leu Glu Ile Phe Lys Phe Arg Asn Phe Ser Leu         280 285 290 ggc aat atc tgc atc atg gcc atg gga ttc acc gtg gct ggt act cct 1027 Gly Asn Ile Cys Ile Met Ala Met Gly Phe Thr Val Ala Gly Thr Pro     295 300 305 ctg ccc atc atg ttg tac ttc cag caa gca cac gga atg aac gcc atg 1075 Leu Pro Ile Met Leu Tyr Phe Gln Gln Ala His Gly Met Asn Ala Met 310 315 320 325 gaa gcg ggt ttc atg atg gtg cct caa gct ctc atg gca gca gta ctg 1123 Glu Ala Gly Phe Met Met Val Pro Gln Ala Leu Met Ala Ala Val Leu                 330 335 340 tca cca ttt gtt gga aag ctg gtt gat cga tcc aac cct gga ctc atg 1171 Ser Pro Phe Val Gly Lys Leu Val Asp Arg Ser Asn Pro Gly Leu Met             345 350 355 gca gcc ctc ggt ttt agc aca gtg gct gtg tcc att gta ctg ctg tca 1219 Ala Ala Leu Gly Phe Ser Thr Val Ala Val Ser Ile Val Leu Leu Ser         360 365 370 atg gta atg att ttc gat acg ggt cta gtc tgg gca ctt gtt tcg atg 1267 Met Val Met Ile Phe Asp Thr Gly Leu Val Trp Ala Leu Val Ser Met     375 380 385 act ttg ctc ggc atc gga aac gcc ttt gtg tgg gca ccg aac tcg acc 1315 Thr Leu Leu Gly Ile Gly Asn Ala Phe Val Trp Ala Pro Asn Ser Thr 390 395 400 405 tcc act atg cgc gac ctg cca cac aag ttc atg gga gcg ggc tct ggc 1363 Ser Thr Met Arg Asp Leu Pro His Lys Phe Met Gly Ala Gly Ser Gly                 410 415 420 gtg ttc aat aca acc cgc caa tta ggt tca gtc atc ggc gcc gct gcc 1411 Val Phe Asn Thr Thr Arg Gln Leu Gly Ser Val Ile Gly Ala Ala Ala             425 430 435 atc ggc gcg gta atg cag att cga ctg gca gca ggc gat gag ggc gca 1459 Ile Gly Ala Val Met Gln Ile Arg Leu Ala Ala Gly Asp Glu Gly Ala         440 445 450 gct ttt ggt caa gca ctt cta ctt gcc gct gcg gtg ctg gtt atc ggc 1507 Ala Phe Gly Gln Ala Leu Leu Leu Ala Ala Ala Val Leu Val Ile Gly     455 460 465 att gtg gca tca acg atg gca gga aaa aat gca cac cca gcg ccg gta 1555 Ile Val Ala Ser Thr Met Ala Gly Lys Asn Ala His Pro Ala Pro Val 470 475 480 485 aag cct taaaggtcgc atgaatcctt cga 1584 Lys pro          <210> 238 <211> 487 <212> PRT <213> Corynebacterium glutamicum <400> 238 Val Ser Asp Lys Lys Gln Asp Leu Thr Ser Ser Ala Ala Gly Ser Ala   1 5 10 15 Ala Pro Gln Thr Lys Ala Tyr Pro Ala Met Pro Leu Pro Glu Lys Gln              20 25 30 Ala Trp Pro Ala Leu Ile Ala Leu Cys Ile Gly Phe Phe Met Ile Leu          35 40 45 Leu Asp Gln Thr Ile Val Ala Val Ser Thr Pro Ala Leu Gln Ala Asp      50 55 60 Met Gly Ala Ser Tyr Asn Glu Val Ile Trp Val Thr Ser Val Tyr Leu  65 70 75 80 Leu Thr Phe Ala Val Pro Leu Leu Val Thr Gly Arg Leu Gly Asp Lys                  85 90 95 Tyr Gly Pro Lys Asn Val Tyr Val Ala Gly Met Val Ile Phe Thr Val             100 105 110 Ser Ser Leu Ala Cys Gly Leu Ala Pro Asp Met Phe Thr Leu Ile Ile         115 120 125 Ala Arg Gly Val Gln Gly Leu Gly Ala Ala Leu Leu Thr Pro Gln Thr     130 135 140 Met Ala Thr Ile Asn Arg Ile Phe Ala Phe Glu Arg Arg Gly Ala Ala 145 150 155 160 Leu Gly Val Trp Gly Ser Thr Ala Gly Leu Ala Ser Leu Ala Gly Pro                 165 170 175 Ile Leu Gly Gly Val Ile Thr Glu Asn Trp Gly Trp Gln Trp Val Phe             180 185 190 Tyr Ile Asn Val Pro Ile Gly Val Ile Ser Val Ile Ala Val Met Lys         195 200 205 Tyr Val Pro Glu Phe Pro Pro Leu Thr Arg Pro Leu Asp Pro Leu Ser     210 215 220 Ile Val Leu Ser Ile Val Ala Val Phe Phe Leu Val Phe Ala Phe Gln 225 230 235 240 Glu Gly Glu Gly Ala Gly Trp Ala Ala Trp Val Trp Ile Met Ile Val                 245 250 255 Ala Ala Phe Ala Leu Phe Ala Trp Phe Ile Tyr Gln Gln Ser Arg Ala             260 265 270 Glu Lys Ser Gly Asn Asp Pro Leu Val Pro Leu Glu Ile Phe Lys Phe         275 280 285 Arg Asn Phe Ser Leu Gly Asn Ile Cys Ile Met Ala Met Gly Phe Thr     290 295 300 Val Ala Gly Thr Pro Leu Pro Ile Met Leu Tyr Phe Gln Gln Ala His 305 310 315 320 Gly Met Asn Ala Met Glu Ala Gly Phe Met Met Val Pro Gln Ala Leu                 325 330 335 Met Ala Ala Val Leu Ser Pro Phe Val Gly Lys Leu Val Asp Arg Ser             340 345 350 Asn Pro Gly Leu Met Ala Ala Leu Gly Phe Ser Thr Val Ala Val Ser         355 360 365 Ile Val Leu Leu Ser Met Val Met Ile Phe Asp Thr Gly Leu Val Trp     370 375 380 Ala Leu Val Ser Met Thr Leu Leu Gly Ile Gly Asn Ala Phe Val Trp 385 390 395 400 Ala Pro Asn Ser Thr Ser Thr Met Arg Asp Leu Pro His Lys Phe Met                 405 410 415 Gly Ala Gly Ser Gly Val Phe Asn Thr Thr Arg Gln Leu Gly Ser Val             420 425 430 Ile Gly Ala Ala Ala Ile Gly Ala Val Met Gln Ile Arg Leu Ala Ala         435 440 445 Gly Asp Glu Gly Ala Ala Phe Gly Gln Ala Leu Leu Leu Ala Ala Ala     450 455 460 Val Leu Val Ile Gly Ile Val Ala Ser Thr Met Ala Gly Lys Asn Ala 465 470 475 480 His Pro Ala Pro Val Lys Pro                 485 <210> 239 <211> 1455 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (1432) <223> RXN03064 <400> 239 tggagccttg tcttcctcca gcaatcccac aacggagcag gttgggatcc cgagaaatgt 60 tgtcatcatc ttggctgtat tagtttttac agcctttgtc atg atg ttg aat gag 115                                             Met Met Leu Asn Glu                                               1 5 act act ctg gca gtc gcg ttg ccg tcg atc atg gcg gac ttt gac att 163 Thr Thr Leu Ala Val Ala Leu Pro Ser Ile Met Ala Asp Phe Asp Ile                  10 15 20 gag gcg aat act gcg cag tgg ttg ctc act ggt ttt atg ttg acc atg 211 Glu Ala Asn Thr Ala Gln Trp Leu Leu Thr Gly Phe Met Leu Thr Met              25 30 35 gct gtg gtt ctt cca gct act ggt tgg atg ttg gaa cgt ttt acc act 259 Ala Val Val Leu Pro Ala Thr Gly Trp Met Leu Glu Arg Phe Thr Thr          40 45 50 cgt agt gtg ttt att ttc gcc acg gtg gtc ttc ctg atc ggt act gtg 307 Arg Ser Val Phe Ile Phe Ala Thr Val Val Phe Leu Ile Gly Thr Val      55 60 65 acg gct gcg ttg tct cct act ttt gcg att atg ctt gca gcc cgc gtc 355 Thr Ala Ala Leu Ser Pro Thr Phe Ala Ile Met Leu Ala Ala Arg Val  70 75 80 85 gct cag gcg att ggt acc gct gtg atc atg ccg ctg ctg atg act gtc 403 Ala Gln Ala Ile Gly Thr Ala Val Ile Met Pro Leu Leu Met Thr Val                  90 95 100 gcg atg acc gtt gtt cct cca gag cgc cgt ggc gcc gtc atg ggt ttg 451 Ala Met Thr Val Val Pro Pro Glu Arg Arg Gly Ala Val Met Gly Leu             105 110 115 att gcg gtc gtg atg gcc gtt ggt cct gct ctt gga cct agt gtg gct 499 Ile Ala Val Val Met Ala Val Gly Pro Ala Leu Gly Pro Ser Val Ala         120 125 130 ggt ttc gta ctc agc ttg tct tcg tgg cac gcg att ttc tgg gtc atg 547 Gly Phe Val Leu Ser Leu Ser Ser Trp His Ala Ile Phe Trp Val Met     135 140 145 gtt ccg ttg gtg ttt gtg gca agc ctg atc ggt acc ctg cgt ctg acc 595 Val Pro Leu Val Phe Val Ala Ser Leu Ile Gly Thr Leu Arg Leu Thr 150 155 160 165 aac gtc agt gag cct aaa aag act cct ttg gat gtt att tcc ttc ctg 643 Asn Val Ser Glu Pro Lys Lys Thr Pro Leu Asp Val Ile Ser Phe Leu                 170 175 180 att tcc gca gtg gct ttc ggt ggc ctt gtg tac gcc ttg agc tcg att 691 Ile Ser Ala Val Ala Phe Gly Gly Leu Val Tyr Ala Leu Ser Ser Ile             185 190 195 ggc atc att ttg gaa ggt gac aga agc gct ttg gtc gtg ttg gct gtc 739 Gly Ile Ile Leu Glu Gly Asp Arg Ser Ala Leu Val Val Leu Ala Val         200 205 210 ggc atc att gcg ttg gtg gtg ttt gtg tgg cgc cag att gcc atg ggt 787 Gly Ile Ile Ala Leu Val Val Phe Val Trp Arg Gln Ile Ala Met Gly     215 220 225 aag cag gat aag gcg ctg ttg gat ctg cgt ccg ttg gcg att cgt gag 835 Lys Gln Asp Lys Ala Leu Leu Asp Leu Arg Pro Leu Ala Ile Arg Glu 230 235 240 245 tac acc att ccg ctg gtt gtg ctt ttg acg ctg ttc ggt gcg ctg ctc 883 Tyr Thr Ile Pro Leu Val Val Leu Leu Thr Leu Phe Gly Ala Leu Leu                 250 255 260 ggt gtc atg aat aca ctg ccg ctc tac ctg cag gga tcc ttg atg gtc 931 Gly Val Met Asn Thr Leu Pro Leu Tyr Leu Gln Gly Ser Leu Met Val             265 270 275 acc gcc ttg gtc gcg ggt cta gtg ctg ttg cca ggt ggt ctt ttg gaa 979 Thr Ala Leu Val Ala Gly Leu Val Leu Leu Pro Gly Gly Leu Leu Glu         280 285 290 ggt gtg ctg tcg cca ttt gtg ggt cga att tat gat cgt cat ggt cca 1027 Gly Val Leu Ser Pro Phe Val Gly Arg Ile Tyr Asp Arg His Gly Pro     295 300 305 cgc gga ctc gtg atc ggc ggt atg tca ctc gtt gtg atc tcc ctg ttt 1075 Arg Gly Leu Val Ile Gly Gly Met Ser Leu Val Val Ile Ser Leu Phe 310 315 320 325 gca ctg tcc acc gtc gat gag ttc gcc aac gtg tgg ttc atc atc ggc 1123 Ala Leu Ser Thr Val Asp Glu Phe Ala Asn Val Trp Phe Ile Ily Gly                 330 335 340 gta cac atc gtg ttc tcc atc ggc ctt gcg ctg ctg ttc acc cca ctg 1171 Val His Ile Val Phe Ser Ile Gly Leu Ala Leu Leu Phe Thr Pro Leu             345 350 355 atg aca gtc gcg ctc gca tcc gtc ccc gac aac atg tac ggc cac ggc 1219 Met Thr Val Ala Leu Ala Ser Val Pro Asp Asn Met Tyr Gly His Gly         360 365 370 tcc gcg atc ctc aac acc ctc caa cag ctc gcc ggc gcc gca ggc acc 1267 Ser Ala Ile Leu Asn Thr Leu Gln Gln Leu Ala Gly Ala Ala Gly Thr     375 380 385 gcg gtc atg att gcg gtt tat tcc acc gtc agc aac aac gcg ctt atc 1315 Ala Val Met Ile Ala Val Tyr Ser Thr Val Ser Asn Asla Ala Leu Ile 390 395 400 405 gac ggc gca acc caa caa acc gcc ctc gcc gac ggc gcc aac tct gca 1363 Asp Gly Ala Thr Gln Gln Thr Ala Leu Ala Asp Gly Ala Asn Ser Ala                 410 415 420 ttc ttc gcc tca gcg tgc gtg gca gtg ttt gca ctg atc gtg ggc ttc 1411 Phe Phe Ala Ser Ala Cys Val Ala Val Phe Ala Leu Ile Val Gly Phe             425 430 435 ttt gta aag agg cca gcc cgc taagctaggt cgcatgatca gca 1455 Phe Val Lys Arg Pro Ala Arg         440 <210> 240 <211> 444 <212> PRT <213> Corynebacterium glutamicum <400> 240 Met Met Leu Asn Glu Thr Thr Leu Ala Val Ala Leu Pro Ser Ile Met   1 5 10 15 Ala Asp Phe Asp Ile Glu Ala Asn Thr Ala Gln Trp Leu Leu Thr Gly              20 25 30 Phe Met Leu Thr Met Ala Val Val Leu Pro Ala Thr Gly Trp Met Leu          35 40 45 Glu Arg Phe Thr Thr Arg Ser Val Phe Ile Phe Ala Thr Val Val Phe      50 55 60 Leu Ile Gly Thr Val Thr Ala Ala Leu Ser Pro Thr Phe Ala Ile Met  65 70 75 80 Leu Ala Ala Arg Val Ala Gln Ala Ile Gly Thr Ala Val Ile Met Pro                  85 90 95 Leu Leu Met Thr Val Ala Met Thr Val Val Pro Pro Glu Arg Arg Gly             100 105 110 Ala Val Met Gly Leu Ile Ala Val Val Met Ala Val Gly Pro Ala Leu         115 120 125 Gly Pro Ser Val Ala Gly Phe Val Leu Ser Leu Ser Ser Trp His Ala     130 135 140 Ile Phe Trp Val Met Val Pro Leu Val Phe Val Ala Ser Leu Ile Gly 145 150 155 160 Thr Leu Arg Leu Thr Asn Val Ser Glu Pro Lys Lys Thr Pro Leu Asp                 165 170 175 Val Ile Ser Phe Leu Ile Ser Ala Val Ala Phe Gly Gly Leu Val Tyr             180 185 190 Ala Leu Ser Ser Ile Gly Ile Ile Leu Glu Gly Asp Arg Ser Ala Leu         195 200 205 Val Val Leu Ala Val Gly Ile Ile Ala Leu Val Val Phe Val Trp Arg     210 215 220 Gln Ile Ala Met Gly Lys Gln Asp Lys Ala Leu Leu Asp Leu Arg Pro 225 230 235 240 Leu Ala Ile Arg Glu Tyr Thr Ile Pro Leu Val Val Leu Leu Thr Leu                 245 250 255 Phe Gly Ala Leu Leu Gly Val Met Asn Thr Leu Pro Leu Tyr Leu Gln             260 265 270 Gly Ser Leu Met Val Thr Ala Leu Val Ala Gly Leu Val Leu Leu Pro         275 280 285 Gly Gly Leu Leu Glu Gly Val Leu Ser Pro Phe Val Gly Arg Ile Tyr     290 295 300 Asp Arg His Gly Pro Arg Gly Leu Val Ile Gly Gly Met Ser Leu Val 305 310 315 320 Val Ile Ser Leu Phe Ala Leu Ser Thr Val Asp Glu Phe Ala Asn Val                 325 330 335 Trp Phe Ile Ile Gly Val His Ile Val Phe Ser Ile Gly Leu Ala Leu             340 345 350 Leu Phe Thr Pro Leu Met Thr Val Ala Leu Ala Ser Val Pro Asp Asn         355 360 365 Met Tyr Gly His Gly Ser Ala Ile Leu Asn Thr Leu Gln Gln Leu Ala     370 375 380 Gly Ala Ala Gly Thr Ala Val Met Ile Ala Val Tyr Ser Thr Val Ser 385 390 395 400 Asn Asn Ala Leu Ile Asp Gly Ala Thr Gln Gln Thr Ala Leu Ala Asp                 405 410 415 Gly Ala Asn Ser Ala Phe Phe Ala Ser Ala Cys Val Ala Val Phe Ala             420 425 430 Leu Ile Val Gly Phe Phe Val Lys Arg Pro Ala Arg         435 440 <210> 241 <211> 1093 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (101) .. (1093) <223> FRXA00565 <400> 241 tggagccttg tcttcctcca gcaatcccac aacggagcag gttgggatcc cgagaaatgt 60 tgtcatcatc ttggctgtat tagtttttac agcctttgtc atg atg ttg aat gag 115                                             Met Met Leu Asn Glu                                               1 5 act act ctg gca gtc gcg ttg ccg tcg atc atg gcg gac ttt gac att 163 Thr Thr Leu Ala Val Ala Leu Pro Ser Ile Met Ala Asp Phe Asp Ile                  10 15 20 gag gcg aat act gcg cag tgg ttg ctc act ggt ttt atg ttg acc atg 211 Glu Ala Asn Thr Ala Gln Trp Leu Leu Thr Gly Phe Met Leu Thr Met              25 30 35 gct gtg gtt ctt cca gct act ggt tgg atg ttg gaa cgt ttt acc act 259 Ala Val Val Leu Pro Ala Thr Gly Trp Met Leu Glu Arg Phe Thr Thr          40 45 50 cgt agt gtg ttt att ttc gcc acg gtg gtc ttc ctg atc ggt act gtg 307 Arg Ser Val Phe Ile Phe Ala Thr Val Val Phe Leu Ile Gly Thr Val      55 60 65 acg gct gcg ttg tct cct act ttt gcg att atg ctt gca gcc cgc gtc 355 Thr Ala Ala Leu Ser Pro Thr Phe Ala Ile Met Leu Ala Ala Arg Val  70 75 80 85 gct cag gcg att ggt acc gct gtg atc atg ccg ctg ctg atg act gtc 403 Ala Gln Ala Ile Gly Thr Ala Val Ile Met Pro Leu Leu Met Thr Val                  90 95 100 gcg atg acc gtt gtt cct cca gag cgc cgt ggc gcc gtc atg ggt ttg 451 Ala Met Thr Val Val Pro Pro Glu Arg Arg Gly Ala Val Met Gly Leu             105 110 115 att gcg gtc gtg atg gcc gtt ggt cct gct ctt gga cct agt gtg gct 499 Ile Ala Val Val Met Ala Val Gly Pro Ala Leu Gly Pro Ser Val Ala         120 125 130 ggt ttc gta ctc agc ttg tct tcg tgg cac gcg att ttc tgg gtc atg 547 Gly Phe Val Leu Ser Leu Ser Ser Trp His Ala Ile Phe Trp Val Met     135 140 145 gtt ccg ttg gtg ttt gtg gca agc ctg atc ggt acc ctg cgt ctg acc 595 Val Pro Leu Val Phe Val Ala Ser Leu Ile Gly Thr Leu Arg Leu Thr 150 155 160 165 aac gtc agt gag cct aaa aag act cct ttg gat gtt att tcc ttc ctg 643 Asn Val Ser Glu Pro Lys Lys Thr Pro Leu Asp Val Ile Ser Phe Leu                 170 175 180 att tcc gca gtg gct ttc ggt ggc ctt gtg tac gcc ttg agc tcg att 691 Ile Ser Ala Val Ala Phe Gly Gly Leu Val Tyr Ala Leu Ser Ser Ile             185 190 195 ggc atc att ttg gaa ggt gac aga agc gct ttg gtc gtg ttg gct gtc 739 Gly Ile Ile Leu Glu Gly Asp Arg Ser Ala Leu Val Val Leu Ala Val         200 205 210 ggc atc att gcg ttg gtg gtg ttt gtg tgg cgc cag att gcc atg ggt 787 Gly Ile Ile Ala Leu Val Val Phe Val Trp Arg Gln Ile Ala Met Gly     215 220 225 aag cag gat aag gcg ctg ttg gat ctg cgt ccg ttg gcg att cgt gag 835 Lys Gln Asp Lys Ala Leu Leu Asp Leu Arg Pro Leu Ala Ile Arg Glu 230 235 240 245 tac acc att ccg ctg gtt gtg ctt ttg acg ctg ttc ggt gcg ctg ctc 883 Tyr Thr Ile Pro Leu Val Val Leu Leu Thr Leu Phe Gly Ala Leu Leu                 250 255 260 ggt gtc atg aat aca ctg ccg ctc tac ctg cag gga tcc ttg atg gtc 931 Gly Val Met Asn Thr Leu Pro Leu Tyr Leu Gln Gly Ser Leu Met Val             265 270 275 acc gcc ttg gtc gcg ggt cta gtg ctg ttg cca ggt ggt ctt ttg gaa 979 Thr Ala Leu Val Ala Gly Leu Val Leu Leu Pro Gly Gly Leu Leu Glu         280 285 290 ggt gtg ctg tcg cca ttt gtg ggt cga att tat gat cgt cat ggt cca 1027 Gly Val Leu Ser Pro Phe Val Gly Arg Ile Tyr Asp Arg His Gly Pro     295 300 305 cgc gga ctc gtg atc ggc ggt atg tca ctc gtt gtg atc tcc ctg ttt 1075 Arg Gly Leu Val Ile Gly Gly Met Ser Leu Val Val Ile Ser Leu Phe 310 315 320 325 gca ctg tcc acc gtc gat 1093 Ala Leu Ser Thr Val Asp                 330 <210> 242 <211> 331 <212> PRT <213> Corynebacterium glutamicum <400> 242 Met Met Leu Asn Glu Thr Thr Leu Ala Val Ala Leu Pro Ser Ile Met   1 5 10 15 Ala Asp Phe Asp Ile Glu Ala Asn Thr Ala Gln Trp Leu Leu Thr Gly              20 25 30 Phe Met Leu Thr Met Ala Val Val Leu Pro Ala Thr Gly Trp Met Leu          35 40 45 Glu Arg Phe Thr Thr Arg Ser Val Phe Ile Phe Ala Thr Val Val Phe      50 55 60 Leu Ile Gly Thr Val Thr Ala Ala Leu Ser Pro Thr Phe Ala Ile Met  65 70 75 80 Leu Ala Ala Arg Val Ala Gln Ala Ile Gly Thr Ala Val Ile Met Pro                  85 90 95 Leu Leu Met Thr Val Ala Met Thr Val Val Pro Pro Glu Arg Arg Gly             100 105 110 Ala Val Met Gly Leu Ile Ala Val Val Met Ala Val Gly Pro Ala Leu         115 120 125 Gly Pro Ser Val Ala Gly Phe Val Leu Ser Leu Ser Ser Trp His Ala     130 135 140 Ile Phe Trp Val Met Val Pro Leu Val Phe Val Ala Ser Leu Ile Gly 145 150 155 160 Thr Leu Arg Leu Thr Asn Val Ser Glu Pro Lys Lys Thr Pro Leu Asp                 165 170 175 Val Ile Ser Phe Leu Ile Ser Ala Val Ala Phe Gly Gly Leu Val Tyr             180 185 190 Ala Leu Ser Ser Ile Gly Ile Ile Leu Glu Gly Asp Arg Ser Ala Leu         195 200 205 Val Val Leu Ala Val Gly Ile Ile Ala Leu Val Val Phe Val Trp Arg     210 215 220 Gln Ile Ala Met Gly Lys Gln Asp Lys Ala Leu Leu Asp Leu Arg Pro 225 230 235 240 Leu Ala Ile Arg Glu Tyr Thr Ile Pro Leu Val Val Leu Leu Thr Leu                 245 250 255 Phe Gly Ala Leu Leu Gly Val Met Asn Thr Leu Pro Leu Tyr Leu Gln             260 265 270 Gly Ser Leu Met Val Thr Ala Leu Val Ala Gly Leu Val Leu Leu Pro         275 280 285 Gly Gly Leu Leu Glu Gly Val Leu Ser Pro Phe Val Gly Arg Ile Tyr     290 295 300 Asp Arg His Gly Pro Arg Gly Leu Val Ile Gly Gly Met Ser Leu Val 305 310 315 320 Val Ile Ser Leu Phe Ala Leu Ser Thr Val Asp                 325 330 <210> 243 <211> 380 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (1) .. (357) <223> FRXA02878 <400> 243 tgc ctg tcc acc gtc gat gag ttc gcc acg tgt tgg tca tca ttc gcg 48 Cys Leu Ser Thr Val Asp Glu Phe Ala Thr Cys Trp Ser Ser Phe Ala   1 5 10 15 gac aca tcg tgg ttc tca tcg gcc ctt gcg ctg ctg ttc acc cca ctg 96 Asp Thr Ser Trp Phe Ser Ser Ala Leu Ala Leu Leu Phe Thr Pro Leu              20 25 30 atg aca gtc gcg ctc gca tcc gtc ccc gac aac atg tac ggc cac ggc 144 Met Thr Val Ala Leu Ala Ser Val Pro Asp Asn Met Tyr Gly His Gly          35 40 45 tcc gcg atc ctc aac acc ctc caa cag ctc gcc ggc gcc gca ggc acc 192 Ser Ala Ile Leu Asn Thr Leu Gln Gln Leu Ala Gly Ala Ala Gly Thr      50 55 60 gcg gtc atg att gcg gtt tat tcc acc gtc agc aac aac gcg ctt atc 240 Ala Val Met Ile Ala Val Tyr Ser Thr Val Ser Asn Asla Ala Leu Ile  65 70 75 80 gac ggc gca acc caa caa acc gcc ctc gcc gac ggc gcc aac tct gca 288 Asp Gly Ala Thr Gln Gln Thr Ala Leu Ala Asp Gly Ala Asn Ser Ala                  85 90 95 ttc ttc gcc tca gcg tgc gtg gca gtg ttt gca ctg atc gtg ggc ttc 336 Phe Phe Ala Ser Ala Cys Val Ala Val Phe Ala Leu Ile Val Gly Phe             100 105 110 ttt gta aag agg cca gcc cgc taagctaggt cgcatgatca gca 380 Phe Val Lys Arg Pro Ala Arg         115 <210> 244 <211> 119 <212> PRT <213> Corynebacterium glutamicum <400> 244 Cys Leu Ser Thr Val Asp Glu Phe Ala Thr Cys Trp Ser Ser Phe Ala   1 5 10 15 Asp Thr Ser Trp Phe Ser Ser Ala Leu Ala Leu Leu Phe Thr Pro Leu              20 25 30 Met Thr Val Ala Leu Ala Ser Val Pro Asp Asn Met Tyr Gly His Gly          35 40 45 Ser Ala Ile Leu Asn Thr Leu Gln Gln Leu Ala Gly Ala Ala Gly Thr      50 55 60 Ala Val Met Ile Ala Val Tyr Ser Thr Val Ser Asn Asla Ala Leu Ile  65 70 75 80 Asp Gly Ala Thr Gln Gln Thr Ala Leu Ala Asp Gly Ala Asn Ser Ala                  85 90 95 Phe Phe Ala Ser Ala Cys Val Ala Val Phe Ala Leu Ile Val Gly Phe             100 105 110 Phe Val Lys Arg Pro Ala Arg         115 <210> 245 <211> 1533 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (1510) <223> RXA00648 <400> 245 gtttgccagc tattgcatct tgcacaaaat gtgtaccata cacataatgt catcgagtcc 60 tcccgaatca gccacaccac agatcaaata cggcctgctg gtg gtc act ctc gcc 115                                             Val Val Thr Leu Ala                                               1 5 tca gct ggt atc act gtt tcc cta gcg cag acc ctg gtt att ccg atc 163 Ser Ala Gly Ile Thr Val Ser Leu Ala Gln Thr Leu Val Ile Pro Ile                  10 15 20 att ggt cgg ttg ccc gag atc ttc aac acc acg gct gct aat gcc tct 211 Ile Gly Arg Leu Pro Glu Ile Phe Asn Thr Thr Ala Ala Asn Ala Ser              25 30 35 tgg atc att act gtg acg ctg ttg gtg ggc gca gtg gcg act cct gtg 259 Trp Ile Ile Thr Val Thr Leu Leu Val Gly Ala Val Ala Thr Pro Val          40 45 50 atg ggc agg ctt gca gat atg tac ggc aag aaa aag atg atg ctc atc 307 Met Gly Arg Leu Ala Asp Met Tyr Gly Lys Lys Lys Met Met Leu Ile      55 60 65 tca ctt gtc ccg ttc att ctt gga tca gtg atc tgc gct gtg tcg gtg 355 Ser Leu Val Pro Phe Ile Leu Gly Ser Val Ile Cys Ala Val Ser Val  70 75 80 85 gat ttg att ccg atg atc atc ggc cgt ggt ttt cag ggg ctt ggc tct 403 Asp Leu Ile Pro Met Ile Ile Gly Arg Gly Phe Gln Gly Leu Gly Ser                  90 95 100 ggc ctg att cct ctt ggc att tct ctc atg cat gat ttg ttg ccc cgg 451 Gly Leu Ile Pro Leu Gly Ile Ser Leu Met His Asp Leu Leu Pro Arg             105 110 115 gag aaa gca ggg tct gcc att gct ttg atg agt tct tcc atg ggc att 499 Glu Lys Ala Gly Ser Ala Ile Ala Leu Met Ser Ser Ser Met Gly Ile         120 125 130 ggc ggt gca ctc ggt cta ccg ctg gct gct gct att gcc cag ttt gcg 547 Gly Gly Ala Leu Gly Leu Pro Leu Ala Ala Ala Ile Ala Gln Phe Ala     135 140 145 tcc tgg cgg gtg ctg ttc tgg ttc acc gct ctg gta gcg ctt aca gtt 595 Ser Trp Arg Val Leu Phe Trp Phe Thr Ala Leu Val Ala Leu Thr Val 150 155 160 165 ggc gcg gtc att tgg aag gcg att cct gct aga ccc agg atc gtg agg 643 Gly Ala Val Ile Trp Lys Ala Ile Pro Ala Arg Pro Arg Ile Val Arg                 170 175 180 agt ggc ggc ttt gat tat ttc ggt gct ctc ggc ctt gca atg gga ctt 691 Ser Gly Gly Phe Asp Tyr Phe Gly Ala Leu Gly Leu Ala Met Gly Leu             185 190 195 atc gca ttg ttg ctc gcg gtg tcc aag gga tca gaa tgg ggc tgg aga 739 Ile Ala Leu Leu Leu Ala Val Ser Lys Gly Ser Glu Trp Gly Trp Arg         200 205 210 agt gcc ctg acc att ggg tta ttc gtg gca gcg ctg gtg att ttg gtg 787 Ser Ala Leu Thr Ile Gly Leu Phe Val Ala Ala Leu Val Ile Leu Val     215 220 225 ggt tgg ggc tgg ttc gaa acc cgc cag aaa tcc cct ttg att gat ctg 835 Gly Trp Gly Trp Phe Glu Thr Arg Gln Lys Ser Pro Leu Ile Asp Leu 230 235 240 245 cgc acc act att cgg gcg acc gtg ttg atg aca aat att gcg tcc atc 883 Arg Thr Thr Ile Arg Ala Thr Val Leu Met Thr Asn Ile Ala Ser Ile                 250 255 260 ctc atc ggt ttc acc atg tat gga atg aat ctg atc ctg cct cag gtc 931 Leu Ile Gly Phe Thr Met Tyr Gly Met Asn Leu Ile Leu Pro Gln Val             265 270 275 atg cag ctg cct gta att ctg ggc tac ggt cta ggc cag agc atg ctt 979 Met Gln Leu Pro Val Ile Leu Gly Tyr Gly Leu Gly Gln Ser Met Leu         280 285 290 cag atg ggc atc tgg ctg atc ccg atg ggt cta ggc atg atg ttg att 1027 Gln Met Gly Ile Trp Leu Ile Pro Met Gly Leu Gly Met Met Leu Ile     295 300 305 tcg aat gca ggt gca gcc att agc gct gct cat ggt cct cgt gtg acg 1075 Ser Asn Ala Gly Ala Ala Ile Ser Ala Ala His Gly Pro Arg Val Thr 310 315 320 325 ctg aca att gcg ggt gtt gtg atc gca gtc ggt tat gca ctt act gcc 1123 Leu Thr Ile Ala Gly Val Val Ile Ala Val Gly Tyr Ala Leu Thr Ala                 330 335 340 aca gtg ttg ttc act atc ggc aac cgc aca ccg gga gga gat gca gac 1171 Thr Val Leu Phe Thr Ile Gly Asn Arg Thr Pro Gly Gly Asp Ala Asp             345 350 355 aac gca ctt att ttg acc acc ctg gtg ctg ttc tca gtg tgt agt ctc 1219 Asn Ala Leu Ile Leu Thr Thr Leu Val Leu Phe Ser Val Cys Ser Leu         360 365 370 gtg gtc ggt atc ggc att ggc ctg gca ttt ggt tcc atg cct gcc ttg 1267 Val Val Gly Ile Gly Ile Gly Leu Ala Phe Gly Ser Met Pro Ala Leu     375 380 385 atc atg ggt gcc gta cca gcc acg gag aaa gcc gca gcg aat ggt ttc 1315 Ile Met Gly Ala Val Pro Ala Thr Glu Lys Ala Ala Ala Asn Gly Phe 390 395 400 405 aac tct ctt atg cgt tca ctg ggc acc acc ggc tca tca gct gtc atc 1363 Asn Ser Leu Met Arg Ser Leu Gly Thr Thr Gly Ser Ser Ala Val Ile                 410 415 420 ggt gca gtg ttg gcc gga atg atg agt ggc gga gta ccc acc tta ggg 1411 Gly Ala Val Leu Ala Gly Met Met Ser Gly Gly Val Pro Thr Leu Gly             425 430 435 gga ttc atg acc act ctg atc atc gga tgc tgc gcc gcg ctt gtg gct 1459 Gly Phe Met Thr Thr Leu Ile Ile Gly Cys Cys Ala Ala Leu Val Ala         440 445 450 gcg gtc atc tcc tat ttc atc ccc acc aca acc act gtg gtg gaa gca 1507 Ala Val Ile Ser Tyr Phe Ile Pro Thr Thr Thr Thr Thr Val Val Glu Ala     455 460 465 aaa taatcccggc agcgactcga cca 1533 Lys 470 <210> 246 <211> 470 <212> PRT <213> Corynebacterium glutamicum <400> 246 Val Val Thr Leu Ala Ser Ala Gly Ile Thr Val Ser Leu Ala Gln Thr   1 5 10 15 Leu Val Ile Pro Ile Ile Gly Arg Leu Pro Glu Ile Phe Asn Thr Thr              20 25 30 Ala Ala Asn Ala Ser Trp Ile Ile Thr Val Thr Leu Leu Val Gly Ala          35 40 45 Val Ala Thr Pro Val Met Gly Arg Leu Ala Asp Met Tyr Gly Lys Lys      50 55 60 Lys Met Met Leu Ile Ser Leu Val Pro Phe Ile Leu Gly Ser Val Ile  65 70 75 80 Cys Ala Val Ser Val Asp Leu Ile Pro Met Ile Ile Gly Arg Gly Phe                  85 90 95 Gln Gly Leu Gly Ser Gly Leu Ile Pro Leu Gly Ile Ser Leu Met His             100 105 110 Asp Leu Leu Pro Arg Glu Lys Ala Gly Ser Ala Ile Ala Leu Met Ser         115 120 125 Ser Ser Met Gly Ile Gly Gly Ala Leu Gly Leu Pro Leu Ala Ala Ala     130 135 140 Ile Ala Gln Phe Ala Ser Trp Arg Val Leu Phe Trp Phe Thr Ala Leu 145 150 155 160 Val Ala Leu Thr Val Gly Ala Val Ile Trp Lys Ala Ile Pro Ala Arg                 165 170 175 Pro Arg Ile Val Arg Ser Gly Gly Phe Asp Tyr Phe Gly Ala Leu Gly             180 185 190 Leu Ala Met Gly Leu Ile Ala Leu Leu Leu Ala Val Ser Lys Gly Ser         195 200 205 Glu Trp Gly Trp Arg Ser Ala Leu Thr Ile Gly Leu Phe Val Ala Ala     210 215 220 Leu Val Ile Leu Val Gly Trp Gly Trp Phe Glu Thr Arg Gln Lys Ser 225 230 235 240 Pro Leu Ile Asp Leu Arg Thr Thr Ile Arg Ala Thr Val Leu Met Thr                 245 250 255 Asn Ile Ala Ser Ile Leu Ile Gly Phe Thr Met Tyr Gly Met Asn Leu             260 265 270 Ile Leu Pro Gln Val Met Gln Leu Pro Val Ile Leu Gly Tyr Gly Leu         275 280 285 Gly Gln Ser Met Leu Gln Met Gly Ile Trp Leu Ile Pro Met Gly Leu     290 295 300 Gly Met Met Leu Ile Ser Asn Ala Gly Ala Ala Ile Ser Ala Ala His 305 310 315 320 Gly Pro Arg Val Thr Leu Thr Ile Ala Gly Val Val Ile Ala Val Gly                 325 330 335 Tyr Ala Leu Thr Ala Thr Val Leu Phe Thr Ile Gly Asn Arg Thr Pro             340 345 350 Gly Gly Asp Ala Asp Asn Ala Leu Ile Leu Thr Thr Leu Val Leu Phe         355 360 365 Ser Val Cys Ser Leu Val Val Gly Ile Gly Ile Gly Leu Ala Phe Gly     370 375 380 Ser Met Pro Ala Leu Ile Met Gly Ala Val Pro Ala Thr Glu Lys Ala 385 390 395 400 Ala Ala Asn Gly Phe Asn Ser Leu Met Arg Ser Leu Gly Thr Thr Gly                 405 410 415 Ser Ser Ala Val Ile Gly Ala Val Leu Ala Gly Met Met Ser Gly Gly             420 425 430 Val Pro Thr Leu Gly Gly Phe Met Thr Thr Leu Ile Ile Gly Cys Cys         435 440 445 Ala Ala Leu Val Ala Ala Val Ile Ser Tyr Phe Ile Pro Thr Thr Thr     450 455 460 Thr Val Val Glu Ala Lys 465 470 <210> 247 <211> 1770 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1747) <223> RXN01320 <400> 247 gtgaatggca cgacatgcca caaggcacgc aagctgattt ccaagcctgc tgtcgcaaag 60 caattaaaaa tacttttctt cttagaggtg gattttcaga atg aca tca cag gtc 115                                             Met Thr Ser Gln Val                                               1 5 aag ccg gac gac gaa cgt ccg gta aca aca att tca aaa agt ggt gca 163 Lys Pro Asp Asp Glu Arg Pro Val Thr Thr Ile Ser Lys Ser Gly Ala                  10 15 20 cct tcg gcc cac acc tca gca cca tat ggt gca gca gca act gaa gaa 211 Pro Ser Ala His Thr Ser Ala Pro Tyr Gly Ala Ala Ala Thr Glu Glu              25 30 35 gct gtc gag gaa aaa acc aaa ggt cgc gtt gga ttt atc atc gca gcc 259 Ala Val Glu Glu Lys Thr Lys Gly Arg Val Gly Phe Ile Ala Ala          40 45 50 ctc atg ttg gcg atg ctt ctt agc tcc ttg ggt cag acc att ttc ggt 307 Leu Met Leu Ala Met Leu Leu Ser Ser Leu Gly Gln Thr Ile Phe Gly      55 60 65 tct gcc ctg cca acg att gtt ggt gag ctt ggc ggc gtt aac cac atg 355 Ser Ala Leu Pro Thr Ile Val Gly Glu Leu Gly Gly Val Asn His Met  70 75 80 85 acc tgg gtg att acc gcc ttc ctc ttg ggc cag acc att tca ttg cct 403 Thr Trp Val Ile Thr Ala Phe Leu Leu Gly Gln Thr Ile Ser Leu Pro                  90 95 100 att ttc ggc aag ttg ggt gac cag ttt ggt cgc aaa tac ctc ttc atg 451 Ile Phe Gly Lys Leu Gly Asp Gln Phe Gly Arg Lys Tyr Leu Phe Met             105 110 115 ttt gcc atc gca ctg ttc gtg gtg ggt tcc atc atc ggt gct ttg gct 499 Phe Ala Ile Ala Leu Phe Val Val Gly Ser Ile Ile Gly Ala Leu Ala         120 125 130 cag aac atg acc acc ttg att gtg gct cgt gca ctg cag ggt atc gcc 547 Gln Asn Met Thr Thr Leu Ile Val Ala Arg Ala Leu Gln Gly Ile Ala     135 140 145 ggt ggt ggc ttg atg att ctt tct cag gca att acc gct gat gtc acc 595 Gly Gly Gly Leu Met Ile Leu Ser Gln Ala Ile Thr Ala Asp Val Thr 150 155 160 165 acc gcc cgt gag cgt gca aag tac atg ggc atc atg ggt tcc gtt ttc 643 Thr Ala Arg Glu Arg Ala Lys Tyr Met Gly Ile Met Gly Ser Val Phe                 170 175 180 gga ctg tcc tcc atc ctt ggc cca ttg ctt ggt ggc tgg ttc act gac 691 Gly Leu Ser Ser Ile Leu Gly Pro Leu Leu Gly Gly Trp Phe Thr Asp             185 190 195 ggt cca ggc tgg cgt tgg ggt ctg tgg ttg aac gtt cca atc ggc atc 739 Gly Pro Gly Trp Arg Trp Gly Leu Trp Leu Asn Val Pro Ile Gly Ile         200 205 210 atc gca ctg gtt gct atc gct gtg ctg ctg aaa ctt cca gct cgt gaa 787 Ile Ala Leu Val Ala Ile Ala Val Leu Leu Lys Leu Pro Ala Arg Glu     215 220 225 cgt ggc aag gtc tcc gtt gac tgg ttg gga agc atc ttc atg gct atc 835 Arg Gly Lys Val Ser Val Asp Trp Leu Gly Ser Ile Phe Met Ala Ile 230 235 240 245 gcc acc acc gca ttt gtc ctc gca gtg acc tgg ggt ggc aat gaa tat 883 Ala Thr Thr Ala Phe Val Leu Ala Val Thr Trp Gly Gly Asn Glu Tyr                 250 255 260 gag tgg gca tca cca atg atc atc ggt ttg ttc atc acg aca ttg gtc 931 Glu Trp Ala Ser Pro Met Ile Ile Gly Leu Phe Ile Thr Thr Leu Val             265 270 275 gct gcg ata gtg ttc gtt ttc gtc gaa aag cgt gct gtt gac cca ctg 979 Ala Ala Ile Val Phe Val Phe Val Glu Lys Arg Ala Val Asp Pro Leu         280 285 290 gtc ccc atg ggc ctt ttc tcg aac cgc aac ttc gtg ctc acc gcc gtc 1027 Val Pro Met Gly Leu Phe Ser Asn Arg Asn Phe Val Leu Thr Ala Val     295 300 305 gcc ggt atc ggc gta ggc ctg ttt atg atg ggc acc atc gcg tac atg 1075 Ala Gly Ile Gly Val Gly Leu Phe Met Met Gly Thr Ile Ala Tyr Met 310 315 320 325 cct acc tac ctg cag atg gtt cat ggt ctg aac cca acg caa gct ggt 1123 Pro Thr Tyr Leu Gln Met Val His Gly Leu Asn Pro Thr Gln Ala Gly                 330 335 340 ctg atg ctg atc cca atg atg atc ggc ctg att ggt aca tcc act gtg 1171 Leu Met Leu Ile Pro Met Met Ile Gly Leu Ile Gly Thr Ser Thr Val             345 350 355 gtg ggc aac atc gtg tcc aag act ggc aag tac aag tgg tac cca ttc 1219 Val Gly Asn Ile Val Ser Lys Thr Gly Lys Tyr Lys Trp Tyr Pro Phe         360 365 370 atc ggc atg ctc atc atg gtc ctt gcc cta gta ctg cta tcg acg ctg 1267 Ile Gly Met Leu Ile Met Val Leu Ala Leu Val Leu Leu Ser Thr Leu     375 380 385 aca cct tcg gca agc ttg gct ctc att gga ctg tac ttc ttc gtc ttc 1315 Thr Pro Ser Ala Ser Leu Ala Leu Ile Gly Leu Tyr Phe Phe Val Phe 390 395 400 405 gga ttc ggc ctg ggc tgt gca atg cag att ttg gtt ctc atc gtg cag 1363 Gly Phe Gly Leu Gly Cys Ala Met Gln Ile Leu Val Leu Ile Val Gln                 410 415 420 aac tcc ttc cca atc acc atg gtt ggc acc gcg acc ggt tcc aac aac 1411 Asn Ser Phe Pro Ile Thr Met Val Gly Thr Ala Thr Gly Ser Asn Asn             425 430 435 ttc ttc cgc caa atc ggt gga gca gta ggt tcc gca ctg atc ggt ggc 1459 Phe Phe Arg Gln Ile Gly Gly Ala Val Gly Ser Ala Leu Ile Gly Gly         440 445 450 ctg ttt atc tcc aac ctg tcc gac cga ttc acc gaa aac gtc ccc gca 1507 Leu Phe Ile Ser Asn Leu Ser Asp Arg Phe Thr Glu Asn Val Pro Ala     455 460 465 gca gtg gct tcc atg ggt gaa gaa ggc gca caa tac gcc tca gca atg 1555 Ala Val Ala Ser Met Gly Glu Glu Gly Ala Gln Tyr Ala Ser Ala Met 470 475 480 485 tcc gat ttc tcc ggt gca tcc aac ctc act cca cac ctt gtt gaa tca 1603 Ser Asp Phe Ser Gly Ala Ser Asn Leu Thr Pro His Leu Val Glu Ser                 490 495 500 ctt cca caa gca ctc cgt gaa gca att caa ctt tct tac aac gac gcc 1651 Leu Pro Gln Ala Leu Arg Glu Ala Ile Gln Leu Ser Tyr Asn Asp Ala             505 510 515 ctg aca cca atc ttc ttg gcg ctc acc ccg atc gca gta gtc gcc gcg 1699 Leu Thr Pro Ile Phe Leu Ala Leu Thr Pro Ile Ala Val Val Ala Ala         520 525 530 atc ctc ctc ttt ttc atc cgt gaa gat cac ctc aag gaa acg cac gaa 1747 Ile Leu Leu Phe Phe Ile Arg Glu Asp His Leu Lys Glu Thr His Glu     535 540 545 taatgacaca cgaaacttcc gtc 1770 <210> 248 <211> 549 <212> PRT <213> Corynebacterium glutamicum <400> 248 Met Thr Ser Gln Val Lys Pro Asp Asp Glu Arg Pro Val Thr Thr Ile   1 5 10 15 Ser Lys Ser Gly Ala Pro Ser Ala His Thr Ser Ala Pro Tyr Gly Ala              20 25 30 Ala Ala Thr Glu Glu Ala Val Glu Glu Lys Thr Lys Gly Arg Val Gly          35 40 45 Phe Ile Ile Ala Ala Leu Met Leu Ala Met Leu Leu Ser Ser Leu Gly      50 55 60 Gln Thr Ile Phe Gly Ser Ala Leu Pro Thr Ile Val Gly Glu Leu Gly  65 70 75 80 Gly Val Asn His Met Thr Trp Val Ile Thr Ala Phe Leu Leu Gly Gln                  85 90 95 Thr Ile Ser Leu Pro Ile Phe Gly Lys Leu Gly Asp Gln Phe Gly Arg             100 105 110 Lys Tyr Leu Phe Met Phe Ala Ile Ala Leu Phe Val Val Gly Ser Ile         115 120 125 Ile Gly Ala Leu Ala Gln Asn Met Thr Thr Leu Ile Val Ala Arg Ala     130 135 140 Leu Gln Gly Ile Ala Gly Gly Gly Leu Met Ile Leu Ser Gln Ala Ile 145 150 155 160 Thr Ala Asp Val Thr Thr Ala Arg Glu Arg Ala Lys Tyr Met Gly Ile                 165 170 175 Met Gly Ser Val Phe Gly Leu Ser Ser Ile Leu Gly Pro Leu Leu Gly             180 185 190 Gly Trp Phe Thr Asp Gly Pro Gly Trp Arg Trp Gly Leu Trp Leu Asn         195 200 205 Val Pro Ile Gly Ile Ile Ala Leu Val Ala Ile Ala Val Leu Leu Lys     210 215 220 Leu Pro Ala Arg Glu Arg Gly Lys Val Ser Val Asp Trp Leu Gly Ser 225 230 235 240 Ile Phe Met Ala Ile Ala Thr Thr Ala Phe Val Leu Ala Val Thr Trp                 245 250 255 Gly Gly Asn Glu Tyr Glu Trp Ala Ser Pro Met Ile Ile Gly Leu Phe             260 265 270 Ile Thr Thr Leu Val Ala Ala Ile Val Phe Val Phe Val Glu Lys Arg         275 280 285 Ala Val Asp Pro Leu Val Pro Met Gly Leu Phe Ser Asn Arg Asn Phe     290 295 300 Val Leu Thr Ala Val Ala Gly Ile Gly Val Gly Leu Phe Met Met Gly 305 310 315 320 Thr Ile Ala Tyr Met Pro Thr Tyr Leu Gln Met Val His Gly Leu Asn                 325 330 335 Pro Thr Gln Ala Gly Leu Met Leu Ile Pro Met Met Ile Gly Leu Ile             340 345 350 Gly Thr Ser Thr Val Val Gly Asn Ile Val Ser Lys Thr Gly Lys Tyr         355 360 365 Lys Trp Tyr Pro Phe Ile Gly Met Leu Ile Met Val Leu Ala Leu Val     370 375 380 Leu Leu Ser Thr Leu Thr Pro Ser Ala Ser Leu Ala Leu Ile Gly Leu 385 390 395 400 Tyr Phe Phe Val Phe Gly Phe Gly Leu Gly Cys Ala Met Gln Ile Leu                 405 410 415 Val Leu Ile Val Gln Asn Ser Phe Pro Ile Thr Met Val Gly Thr Ala             420 425 430 Thr Gly Ser Asn Asn Phe Phe Arg Gln Ile Gly Gly Ala Val Gly Ser         435 440 445 Ala Leu Ile Gly Gly Leu Phe Ile Ser Asn Leu Ser Asp Arg Phe Thr     450 455 460 Glu Asn Val Pro Ala Ala Val Ala Ser Met Gly Glu Glu Gly Ala Gln 465 470 475 480 Tyr Ala Ser Ala Met Ser Asp Phe Ser Gly Ala Ser Asn Leu Thr Pro                 485 490 495 His Leu Val Glu Ser Leu Pro Gln Ala Leu Arg Glu Ala Ile Gln Leu             500 505 510 Ser Tyr Asn Asp Ala Leu Thr Pro Ile Phe Leu Ala Leu Thr Pro Ile         515 520 525 Ala Val Val Ala Ala Ile Leu Leu Phe Phe Ile Arg Glu Asp His Leu     530 535 540 Lys Glu Thr His Glu 545 <210> 249 <211> 841 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (841) <223> FRXA01314 <400> 249 gtgaatggca cgacatgcca caaggcacgc aagctgattt ccaagcctgc tgtcgcaaag 60 caattaaaaa tacttttctt cttagaggtg gattttcaga atg aca tca cag gtc 115                                             Met Thr Ser Gln Val                                               1 5 aag ccg gac gac gaa cgt ccg gta aca aca att tca aaa agt ggt gca 163 Lys Pro Asp Asp Glu Arg Pro Val Thr Thr Ile Ser Lys Ser Gly Ala                  10 15 20 cct tcg gcc cac acc tca gca cca tat ggt gca gca gca act gaa gaa 211 Pro Ser Ala His Thr Ser Ala Pro Tyr Gly Ala Ala Ala Thr Glu Glu              25 30 35 gct gtc gag gaa aaa acc aaa ggt cgc gtt gga ttt atc atc gca gcc 259 Ala Val Glu Glu Lys Thr Lys Gly Arg Val Gly Phe Ile Ala Ala          40 45 50 ctc atg ttg gcg atg ctt ctt agc tcc ttg ggt cag acc att ttc ggt 307 Leu Met Leu Ala Met Leu Leu Ser Ser Leu Gly Gln Thr Ile Phe Gly      55 60 65 tct gcc ctg cca acg att gtt ggt gag ctt ggc ggc gtt aac cac atg 355 Ser Ala Leu Pro Thr Ile Val Gly Glu Leu Gly Gly Val Asn His Met  70 75 80 85 acc tgg gtg att acc gcc ttc ctc ttg ggc cag acc att tca ttg cct 403 Thr Trp Val Ile Thr Ala Phe Leu Leu Gly Gln Thr Ile Ser Leu Pro                  90 95 100 att ttc ggc aag ttg ggt gac cag ttt ggt cgc aaa tac ctc ttc atg 451 Ile Phe Gly Lys Leu Gly Asp Gln Phe Gly Arg Lys Tyr Leu Phe Met             105 110 115 ttt gcc atc gca ctg ttc gtg gtg ggt tcc atc atc ggt gct ttg gct 499 Phe Ala Ile Ala Leu Phe Val Val Gly Ser Ile Ile Gly Ala Leu Ala         120 125 130 cag aac atg acc acc ttg att gtg gct cgt gca ctg cag ggt atc gcc 547 Gln Asn Met Thr Thr Leu Ile Val Ala Arg Ala Leu Gln Gly Ile Ala     135 140 145 ggt ggt ggc ttg atg att ctt tct cag gca att acc gct gat gtc acc 595 Gly Gly Gly Leu Met Ile Leu Ser Gln Ala Ile Thr Ala Asp Val Thr 150 155 160 165 acc gcc cgt gag cgt gca aag tac atg ggc atc atg ggt tcc gtt ttc 643 Thr Ala Arg Glu Arg Ala Lys Tyr Met Gly Ile Met Gly Ser Val Phe                 170 175 180 gga ctg tcc tcc atc ctt ggc cca ttg ctt ggt ggc tgg ttc act gac 691 Gly Leu Ser Ser Ile Leu Gly Pro Leu Leu Gly Gly Trp Phe Thr Asp             185 190 195 ggt cca ggc tgg cgt tgg ggt ctg tgg ttg aac gtt cca atc ggc atc 739 Gly Pro Gly Trp Arg Trp Gly Leu Trp Leu Asn Val Pro Ile Gly Ile         200 205 210 atc gca ctg gtt gct atc gct gtg ctg ctg aaa ctt cca gct cgt gaa 787 Ile Ala Leu Val Ala Ile Ala Val Leu Leu Lys Leu Pro Ala Arg Glu     215 220 225 cgt ggc aag gtc tcc gtt gac tgg ttg gga agc atc ttc atg gct atc 835 Arg Gly Lys Val Ser Val Asp Trp Leu Gly Ser Ile Phe Met Ala Ile 230 235 240 245 gcc acc 841 Ala thr          <210> 250 <211> 247 <212> PRT <213> Corynebacterium glutamicum <400> 250 Met Thr Ser Gln Val Lys Pro Asp Asp Glu Arg Pro Val Thr Thr Ile   1 5 10 15 Ser Lys Ser Gly Ala Pro Ser Ala His Thr Ser Ala Pro Tyr Gly Ala              20 25 30 Ala Ala Thr Glu Glu Ala Val Glu Glu Lys Thr Lys Gly Arg Val Gly          35 40 45 Phe Ile Ile Ala Ala Leu Met Leu Ala Met Leu Leu Ser Ser Leu Gly      50 55 60 Gln Thr Ile Phe Gly Ser Ala Leu Pro Thr Ile Val Gly Glu Leu Gly  65 70 75 80 Gly Val Asn His Met Thr Trp Val Ile Thr Ala Phe Leu Leu Gly Gln                  85 90 95 Thr Ile Ser Leu Pro Ile Phe Gly Lys Leu Gly Asp Gln Phe Gly Arg             100 105 110 Lys Tyr Leu Phe Met Phe Ala Ile Ala Leu Phe Val Val Gly Ser Ile         115 120 125 Ile Gly Ala Leu Ala Gln Asn Met Thr Thr Leu Ile Val Ala Arg Ala     130 135 140 Leu Gln Gly Ile Ala Gly Gly Gly Leu Met Ile Leu Ser Gln Ala Ile 145 150 155 160 Thr Ala Asp Val Thr Thr Ala Arg Glu Arg Ala Lys Tyr Met Gly Ile                 165 170 175 Met Gly Ser Val Phe Gly Leu Ser Ser Ile Leu Gly Pro Leu Leu Gly             180 185 190 Gly Trp Phe Thr Asp Gly Pro Gly Trp Arg Trp Gly Leu Trp Leu Asn         195 200 205 Val Pro Ile Gly Ile Ile Ala Leu Val Ala Ile Ala Val Leu Leu Lys     210 215 220 Leu Pro Ala Arg Glu Arg Gly Lys Val Ser Val Asp Trp Leu Gly Ser 225 230 235 240 Ile Phe Met Ala Ile Ala Thr                 245 <210> 251 <211> 803 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (1) .. (780) <223> FRXA01320 <400> 251 gtt gac cca ctg gtc ccc atg ggc ctt ttc tcg aac cgc aac ttc gtg 48 Val Asp Pro Leu Val Pro Met Gly Leu Phe Ser Asn Arg Asn Phe Val   1 5 10 15 ctc acc gcc gtc gcc ggt atc ggc gta ggc ctg ttt atg atg ggc acc 96 Leu Thr Ala Val Ala Gly Ile Gly Val Gly Leu Phe Met Met Gly Thr              20 25 30 atc gcg tac atg cct acc tac ctg cag atg gtt cat ggt ctg aac cca 144 Ile Ala Tyr Met Pro Thr Tyr Leu Gln Met Val His Gly Leu Asn Pro          35 40 45 acg caa gct ggt ctg atg ctg atc cca atg atg atc ggc ctg att ggt 192 Thr Gln Ala Gly Leu Met Leu Ile Pro Met Met Ile Gly Leu Ile Gly      50 55 60 aca tcc act gtg gtg ggc aac atc gtg tcc aag act ggc aag tac aag 240 Thr Ser Thr Val Val Gly Asn Ile Val Ser Lys Thr Gly Lys Tyr Lys  65 70 75 80 tgg tac cca ttc atc ggc atg ctc atc atg gtc ctt gcc cta gta ctg 288 Trp Tyr Pro Phe Ile Gly Met Leu Ile Met Val Leu Ala Leu Val Leu                  85 90 95 cta tcg acg ctg aca cct tcg gca agc ttg gct ctc att gga ctg tac 336 Leu Ser Thr Leu Thr Pro Ser Ala Ser Leu Ala Leu Ile Gly Leu Tyr             100 105 110 ttc ttc gtc ttc gga ttc ggc ctg ggc tgt gca atg cag att ttg gtt 384 Phe Phe Val Phe Gly Phe Gly Leu Gly Cys Ala Met Gln Ile Leu Val         115 120 125 ctc atc gtg cag aac tcc ttc cca atc acc atg gtt ggc acc gcg acc 432 Leu Ile Val Gln Asn Ser Phe Pro Ile Thr Met Val Gly Thr Ala Thr     130 135 140 ggt tcc aac aac ttc ttc cgc caa atc ggt gga gca gta ggt tcc gca 480 Gly Ser Asn Asn Phe Phe Arg Gln Ile Gly Gly Ala Val Gly Ser Ala 145 150 155 160 ctg atc ggt ggc ctg ttt atc tcc aac ctg tcc gac cga ttc acc gaa 528 Leu Ile Gly Gly Leu Phe Ile Ser Asn Leu Ser Asp Arg Phe Thr Glu                 165 170 175 aac gtc ccc gca gca gtg gct tcc atg ggt gaa gaa ggc gca caa tac 576 Asn Val Pro Ala Ala Val Ala Ser Met Gly Glu Glu Gly Ala Gln Tyr             180 185 190 gcc tca gca atg tcc gat ttc tcc ggt gca tcc aac ctc act cca cac 624 Ala Ser Ala Met Ser Asp Phe Ser Gly Ala Ser Asn Leu Thr Pro His         195 200 205 ctt gtt gaa tca ctt cca caa gca ctc cgt gaa gca att caa ctt tct 672 Leu Val Glu Ser Leu Pro Gln Ala Leu Arg Glu Ala Ile Gln Leu Ser     210 215 220 tac aac gac gcc ctg aca cca atc ttc ttg gcg ctc acc ccg atc gca 720 Tyr Asn Asp Ala Leu Thr Pro Ile Phe Leu Ala Leu Thr Pro Ile Ala 225 230 235 240 gta gtc gcc gcg atc ctc ctc ttt ttc atc cgt gaa gat cac ctc aag 768 Val Val Ala Ala Ile Leu Leu Phe Phe Ile Arg Glu Asp His Leu Lys                 245 250 255 gaa acg cac gaa taatgacaca cgaaacttcc gtc 803 Glu Thr His Glu             260 <210> 252 <211> 260 <212> PRT <213> Corynebacterium glutamicum <400> 252 Val Asp Pro Leu Val Pro Met Gly Leu Phe Ser Asn Arg Asn Phe Val   1 5 10 15 Leu Thr Ala Val Ala Gly Ile Gly Val Gly Leu Phe Met Met Gly Thr              20 25 30 Ile Ala Tyr Met Pro Thr Tyr Leu Gln Met Val His Gly Leu Asn Pro          35 40 45 Thr Gln Ala Gly Leu Met Leu Ile Pro Met Met Ile Gly Leu Ile Gly      50 55 60 Thr Ser Thr Val Val Gly Asn Ile Val Ser Lys Thr Gly Lys Tyr Lys  65 70 75 80 Trp Tyr Pro Phe Ile Gly Met Leu Ile Met Val Leu Ala Leu Val Leu                  85 90 95 Leu Ser Thr Leu Thr Pro Ser Ala Ser Leu Ala Leu Ile Gly Leu Tyr             100 105 110 Phe Phe Val Phe Gly Phe Gly Leu Gly Cys Ala Met Gln Ile Leu Val         115 120 125 Leu Ile Val Gln Asn Ser Phe Pro Ile Thr Met Val Gly Thr Ala Thr     130 135 140 Gly Ser Asn Asn Phe Phe Arg Gln Ile Gly Gly Ala Val Gly Ser Ala 145 150 155 160 Leu Ile Gly Gly Leu Phe Ile Ser Asn Leu Ser Asp Arg Phe Thr Glu                 165 170 175 Asn Val Pro Ala Ala Val Ala Ser Met Gly Glu Glu Gly Ala Gln Tyr             180 185 190 Ala Ser Ala Met Ser Asp Phe Ser Gly Ala Ser Asn Leu Thr Pro His         195 200 205 Leu Val Glu Ser Leu Pro Gln Ala Leu Arg Glu Ala Ile Gln Leu Ser     210 215 220 Tyr Asn Asp Ala Leu Thr Pro Ile Phe Leu Ala Leu Thr Pro Ile Ala 225 230 235 240 Val Val Ala Ala Ile Leu Leu Phe Phe Ile Arg Glu Asp His Leu Lys                 245 250 255 Glu Thr His Glu             260 <210> 253 <211> 1755 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1732) <223> RXN02926 <400> 253 ccctgacacc aatcttcttg gcgctcaccc cgatcgcagt agtcgccgcg atcctcctct 60 ttttcatccg tgaagatcac ctcaaggaaa cgcacgaata atg aca cac gaa act 115                                             Met Thr His Glu Thr                                               1 5 tcc gtc ccc gga cct gcc gac gcg cag gtc gca gga gat acg aag ctg 163 Ser Val Pro Gly Pro Ala Asp Ala Gln Val Ala Gly Asp Thr Lys Leu                  10 15 20 cgc aaa ggc cgc gcg aag aag gaa aaa act cct tca tca atg acg cct 211 Arg Lys Gly Arg Ala Lys Lys Glu Lys Thr Pro Ser Ser Met Thr Pro              25 30 35 gaa caa caa aag aaa gtc tgg tgg gtc ctc agc gcg ctg atg gtc gcc 259 Glu Gln Gln Lys Lys Val Trp Trp Val Leu Ser Ala Leu Met Val Ala          40 45 50 atg atg atg gcc tcc ctt gac cag atg att ttc ggc aca gcc ctg cca 307 Met Met Met Ala Ser Leu Asp Gln Met Ile Phe Gly Thr Ala Leu Pro      55 60 65 aca atc gtc ggt gaa ctc ggc ggc gtt gac cac atg atg tgg gtc atc 355 Thr Ile Val Gly Glu Leu Gly Gly Val Asp His Met Met Trp Val Ile  70 75 80 85 acc gca tac cta ctt gcc gaa acc atc atg ctg ccg atc tac gga aag 403 Thr Ala Tyr Leu Leu Ala Glu Thr Ile Met Leu Pro Ile Tyr Gly Lys                  90 95 100 ctc ggc gac ctg gtt gga cgt aaa ggt ctc ttc atc gga gcc ctc ggc 451 Leu Gly Asp Leu Val Gly Arg Lys Gly Leu Phe Ile Gly Ala Leu Gly             105 110 115 atc ttc ctg atc ggc tcc gtc atc ggc ggg ctt gca gga aat atg acc 499 Ile Phe Leu Ile Gly Ser Val Ile Gly Gly Leu Ala Gly Asn Met Thr         120 125 130 tgg ttg atc gtc ggc cgt gcc gta cag ggc atc ggt ggc ggt gga ctg 547 Trp Leu Ile Val Gly Arg Ala Val Gln Gly Ile Gly Gly Gly Gly Leu     135 140 145 atg atc ctc tcg cag gca atc atc gcg gac gtt gtt cca gca cgt gaa 595 Met Ile Leu Ser Gln Ala Ile Ile Ala Asp Val Val Pro Ala Arg Glu 150 155 160 165 cgt ggc cgc tac atg ggt gtc atg ggt gga gtc ttc gga ctc tct gca 643 Arg Gly Arg Tyr Met Gly Val Met Gly Gly Val Phe Gly Leu Ser Ala                 170 175 180 gtt ctt ggc cca cta ctc ggt ggc tgg ttc acc gaa gga cca ggc tgg 691 Val Leu Gly Pro Leu Leu Gly Gly Trp Phe Thr Glu Gly Pro Gly Trp             185 190 195 cgc tgg gca ttc tgg atg aac atc cca ctg gga atc atc gcc atc ggt 739 Arg Trp Ala Phe Trp Met Asn Ile Pro Leu Gly Ile Ile Ala Ile Gly         200 205 210 gtc gcc att tac ttc ctg gac att cca aag aag agc gtc aag ttc cgc 787 Val Ala Ile Tyr Phe Leu Asp Ile Pro Lys Lys Ser Val Lys Phe Arg     215 220 225 tgg gat tac ctg ggc act ttc ttc atg atc gtt gcc gca acc agc ctg 835 Trp Asp Tyr Leu Gly Thr Phe Phe Met Ile Val Ala Ala Thr Ser Leu 230 235 240 245 atc ctg ttc acc acc tgg ggt gga tcc cag tac gag tgg tct gat cca 883 Ile Leu Phe Thr Thr Trp Gly Gly Ser Gln Tyr Glu Trp Ser Asp Pro                 250 255 260 atc atc att gga ctg atc atc acc acc atc gtt gcc gct gca ctg ctg 931 Ile Ile Ile Gly Leu Ile Ile Thr Thr Ile Val Ala Ala Ala Leu Leu             265 270 275 gtt gtt gtg gaa ctg cgc gca aaa gat cca ttg gtt cca atg tcc ttc 979 Val Val Val Glu Leu Arg Ala Lys Asp Pro Leu Val Pro Met Ser Phe         280 285 290 ttc caa aac cgc aac ttc acg ctc acc acc att gca ggc ctg atc ctg 1027 Phe Gln Asn Arg Asn Phe Thr Leu Thr Thr Ile Ala Gly Leu Ile Leu     295 300 305 ggt atc gca atg ttc ggc atc atc ggc tac ctt ccg acc tac ctc cag 1075 Gly Ile Ala Met Phe Gly Ile Gly Tyr Leu Pro Thr Tyr Leu Gln 310 315 320 325 atg gtc cac gga atc aac gcc acc gaa gcc ggc tac atg ctg atc cca 1123 Met Val His Gly Ile Asn Ala Thr Glu Ala Gly Tyr Met Leu Ile Pro                 330 335 340 atg atg gtc ggc atg atg ggt acc tcc atc tgg act ggt atc cgc atc 1171 Met Met Val Gly Met Met Gly Thr Ser Ile Trp Thr Gly Ile Arg Ile             345 350 355 tcc aac aca gga aag tac aaa ctc ttc cca cca atc ggc atg gtg gtt 1219 Ser Asn Thr Gly Lys Tyr Lys Leu Phe Pro Pro Ile Gly Met Val Val         360 365 370 acc ttc gtg gca ctg atc ttc ttt gcc cga atg gaa gtg tcc acc acc 1267 Thr Phe Val Ala Leu Ile Phe Phe Ala Arg Met Glu Val Ser Thr Thr     375 380 385 ctg tgg cag atc gga atc tac ctc ttc gtc ctc ggc gtc ggc ctg ggt 1315 Leu Trp Gln Ile Gly Ile Tyr Leu Phe Val Leu Gly Val Gly Leu Gly 390 395 400 405 cta gcc atg cag gtt ctg gtc ctg atc gtt cag aac acc ctg cca acc 1363 Leu Ala Met Gln Val Leu Val Leu Ile Val Gln Asn Thr Leu Pro Thr                 410 415 420 gcg gtg gtc gga tcc gca acc gct gtg aac aac ttc ttc cgt caa atc 1411 Ala Val Val Gly Ser Ala Thr Ala Val Asn Asn Phe Phe Arg Gln Ile             425 430 435 ggt tcc tca ctc gga tcc gcg ctg gtc ggt ggc atg ttc gtt ggc aac 1459 Gly Ser Ser Leu Gly Ser Ala Leu Val Gly Gly Met Phe Val Gly Asn         440 445 450 ttg gga acc ctc atg gaa gaa aga atg cca gca gcc atg gca caa ctt 1507 Leu Gly Thr Leu Met Glu Glu Arg Met Pro Ala Ala Met Ala Gln Leu     455 460 465 tca cca gaa gaa caa gcc gcc atg gca gcc caa ggc gga ctg gac tcc 1555 Ser Pro Glu Glu Gln Ala Ala Met Ala Ala Gln Gly Gly Leu Asp Ser 470 475 480 485 aac gaa ttg acg ccg gca atc gtc aat caa ttg cca acc gcg ctc cac 1603 Asn Glu Leu Thr Pro Ala Ile Val Asn Gln Leu Pro Thr Ala Leu His                 490 495 500 gat gcg ttc gcc ggt tcc tac aac gac gca ctc atc cca gtg ttc tac 1651 Asp Ala Phe Ala Gly Ser Tyr Asn Asp Ala Leu Ile Pro Val Phe Tyr             505 510 515 gtg atg atg cca ctg atc ggc atc gcg ctg ctt ctc ttg ctg ttt att 1699 Val Met Met Pro Leu Ile Gly Ile Ala Leu Leu Leu Leu Leu Phe Ile         520 525 530 aag caa gaa aaa cta cgc gaa acc acc aca gac taaacacaaa acaaatgaga 1752 Lys Gln Glu Lys Leu Arg Glu Thr Thr Thr Asp     535 540 cct 1755 <210> 254 <211> 544 <212> PRT <213> Corynebacterium glutamicum <400> 254 Met Thr His Glu Thr Ser Val Pro Gly Pro Ala Asp Ala Gln Val Ala   1 5 10 15 Gly Asp Thr Lys Leu Arg Lys Gly Arg Ala Lys Lys Glu Lys Thr Pro              20 25 30 Ser Ser Met Thr Pro Glu Gln Gln Lys Lys Val Trp Trp Val Leu Ser          35 40 45 Ala Leu Met Val Ala Met Met Met Ala Ser Leu Asp Gln Met Ile Phe      50 55 60 Gly Thr Ala Leu Pro Thr Ile Val Gly Glu Leu Gly Gly Val Asp His  65 70 75 80 Met Met Trp Val Ile Thr Ala Tyr Leu Leu Ala Glu Thr Ile Met Leu                  85 90 95 Pro Ile Tyr Gly Lys Leu Gly Asp Leu Val Gly Arg Lys Gly Leu Phe             100 105 110 Ile Gly Ala Leu Gly Ile Phe Leu Ile Gly Ser Val Ile Gly Gly Leu         115 120 125 Ala Gly Asn Met Thr Trp Leu Ile Val Gly Arg Ala Val Gln Gly Ile     130 135 140 Gly Gly Gly Gly Leu Met Ile Leu Ser Gln Ala Ile Ile Ala Asp Val 145 150 155 160 Val Pro Ala Arg Glu Arg Gly Arg Tyr Met Gly Val Met Gly Gly Val                 165 170 175 Phe Gly Leu Ser Ala Val Leu Gly Pro Leu Leu Gly Gly Trp Phe Thr             180 185 190 Glu Gly Pro Gly Trp Arg Trp Ala Phe Trp Met Asn Ile Pro Leu Gly         195 200 205 Ile Ile Ala Ile Gly Val Ala Ile Tyr Phe Leu Asp Ile Pro Lys Lys     210 215 220 Ser Val Lys Phe Arg Trp Asp Tyr Leu Gly Thr Phe Phe Met Ile Val 225 230 235 240 Ala Ala Thr Ser Leu Ile Leu Phe Thr Thr Trp Gly Gly Ser Gln Tyr                 245 250 255 Glu Trp Ser Asp Pro Ile Ile Ile Gly Leu Ile Ile Thr Thr Ile Val             260 265 270 Ala Ala Ala Leu Leu Val Val Val Glu Leu Arg Ala Lys Asp Pro Leu         275 280 285 Val Pro Met Ser Phe Phe Gln Asn Arg Asn Phe Thr Leu Thr Thr Ile     290 295 300 Ala Gly Leu Ile Leu Gly Ile Ala Met Phe Gly Ile Ile Gly Tyr Leu 305 310 315 320 Pro Thr Tyr Leu Gln Met Val His Gly Ile Asn Ala Thr Glu Ala Gly                 325 330 335 Tyr Met Leu Ile Pro Met Met Val Gly Met Met Gly Thr Ser Ile Trp             340 345 350 Thr Gly Ile Arg Ile Ser Asn Thr Gly Lys Tyr Lys Leu Phe Pro Pro         355 360 365 Ile Gly Met Val Val Thr Phe Val Ala Leu Ile Phe Phe Ala Arg Met     370 375 380 Glu Val Ser Thr Thr Leu Trp Gln Ile Gly Ile Tyr Leu Phe Val Leu 385 390 395 400 Gly Val Gly Leu Gly Leu Ala Met Gln Val Leu Val Leu Ile Val Gln                 405 410 415 Asn Thr Leu Pro Thr Ala Val Val Gly Ser Ala Thr Ala Val Asn Asn             420 425 430 Phe Phe Arg Gln Ile Gly Ser Ser Leu Gly Ser Ala Leu Val Gly Gly         435 440 445 Met Phe Val Gly Asn Leu Gly Thr Leu Met Glu Glu Arg Met Pro Ala     450 455 460 Ala Met Ala Gln Leu Ser Pro Glu Glu Gln Ala Ala Met Ala Ala Gln 465 470 475 480 Gly Gly Leu Asp Ser Asn Glu Leu Thr Pro Ala Ile Val Asn Gln Leu                 485 490 495 Pro Thr Ala Leu His Asp Ala Phe Ala Gly Ser Tyr Asn Asp Ala Leu             500 505 510 Ile Pro Val Phe Tyr Val Met Met Pro Leu Ile Gly Ile Ala Leu Leu         515 520 525 Leu Leu Leu Phe Ile Lys Gln Glu Lys Leu Arg Glu Thr Thr Thr Asp     530 535 540 <210> 255 <211> 1294 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1294) <223> FRXA01319 <400> 255 ccctgacacc aatcttcttg gcgctcaccc cgatcgcagt agtcgccgcg atcctcctct 60 ttttcatccg tgaagatcac ctcaaggaaa cgcacgaata atg aca cac gaa act 115                                             Met Thr His Glu Thr                                               1 5 tcc gtc ccc gga cct gcc gac gcg cag gtc gca gga gat acg aag ctg 163 Ser Val Pro Gly Pro Ala Asp Ala Gln Val Ala Gly Asp Thr Lys Leu                  10 15 20 cgc aaa ggc cgc gcg aag aag gaa aaa act cct tca tca atg acg cct 211 Arg Lys Gly Arg Ala Lys Lys Glu Lys Thr Pro Ser Ser Met Thr Pro              25 30 35 gaa caa caa aag aaa gtc tgg tgg gtc ctc agc gcg ctg atg gtc gcc 259 Glu Gln Gln Lys Lys Val Trp Trp Val Leu Ser Ala Leu Met Val Ala          40 45 50 atg atg atg gcc tcc ctt gac cag atg att ttc ggc aca gcc ctg cca 307 Met Met Met Ala Ser Leu Asp Gln Met Ile Phe Gly Thr Ala Leu Pro      55 60 65 aca atc gtc ggt gaa ctc ggc ggc gtt gac cac atg atg tgg gtc atc 355 Thr Ile Val Gly Glu Leu Gly Gly Val Asp His Met Met Trp Val Ile  70 75 80 85 acc gca tac cta ctt gcc gaa acc atc atg ctg ccg atc tac gga aag 403 Thr Ala Tyr Leu Leu Ala Glu Thr Ile Met Leu Pro Ile Tyr Gly Lys                  90 95 100 ctc ggc gac ctg gtt gga cgt aaa ggt ctc ttc atc gga gcc ctc ggc 451 Leu Gly Asp Leu Val Gly Arg Lys Gly Leu Phe Ile Gly Ala Leu Gly             105 110 115 atc ttc ctg atc ggc tcc gtc atc ggc ggg ctt gca gga aat atg acc 499 Ile Phe Leu Ile Gly Ser Val Ile Gly Gly Leu Ala Gly Asn Met Thr         120 125 130 tgg ttg atc gtc ggc cgt gcc gta cag ggc atc ggt ggc ggt gga ctg 547 Trp Leu Ile Val Gly Arg Ala Val Gln Gly Ile Gly Gly Gly Gly Leu     135 140 145 atg atc ctc tcg cag gca atc atc gcg gac gtt gtt cca gca cgt gaa 595 Met Ile Leu Ser Gln Ala Ile Ile Ala Asp Val Val Pro Ala Arg Glu 150 155 160 165 cgt ggc cgc tac atg ggt gtc atg ggt gga gtc ttc gga ctc tct gca 643 Arg Gly Arg Tyr Met Gly Val Met Gly Gly Val Phe Gly Leu Ser Ala                 170 175 180 gtt ctt ggc cca cta ctc ggt ggc tgg ttc acc gaa gga cca ggc tgg 691 Val Leu Gly Pro Leu Leu Gly Gly Trp Phe Thr Glu Gly Pro Gly Trp             185 190 195 cgc tgg gca ttc tgg atg aac atc cca ctg gga atc atc gcc atc ggt 739 Arg Trp Ala Phe Trp Met Asn Ile Pro Leu Gly Ile Ile Ala Ile Gly         200 205 210 gtc gcc att tac ttc ctg gac att cca aag aag agc gtc aag ttc cgc 787 Val Ala Ile Tyr Phe Leu Asp Ile Pro Lys Lys Ser Val Lys Phe Arg     215 220 225 tgg gat tac ctg ggc act ttc ttc atg atc gtt gcc gca acc agc ctg 835 Trp Asp Tyr Leu Gly Thr Phe Phe Met Ile Val Ala Ala Thr Ser Leu 230 235 240 245 atc ctg ttc acc acc tgg ggt gga tcc cag tac gag tgg tct gat cca 883 Ile Leu Phe Thr Thr Trp Gly Gly Ser Gln Tyr Glu Trp Ser Asp Pro                 250 255 260 atc atc att gga ctg atc atc acc acc atc gtt gcc gct gca ctg ctg 931 Ile Ile Ile Gly Leu Ile Ile Thr Thr Ile Val Ala Ala Ala Leu Leu             265 270 275 gtt gtt gtg gaa ctg cgc gca aaa gat cca ttg gtt cca atg tcc ttc 979 Val Val Val Glu Leu Arg Ala Lys Asp Pro Leu Val Pro Met Ser Phe         280 285 290 ttc caa aac cgc aac ttc acg ctc acc acc att gca ggc ctg atc ctg 1027 Phe Gln Asn Arg Asn Phe Thr Leu Thr Thr Ile Ala Gly Leu Ile Leu     295 300 305 ggt atc gca atg ttc ggc atc atc ggc tac ctt ccg acc tac ctc cag 1075 Gly Ile Ala Met Phe Gly Ile Gly Tyr Leu Pro Thr Tyr Leu Gln 310 315 320 325 atg gtc cac gga atc aac gcc acc gaa gcc ggc tac atg ctg atc cca 1123 Met Val His Gly Ile Asn Ala Thr Glu Ala Gly Tyr Met Leu Ile Pro                 330 335 340 atg atg gtc ggc atg atg ggt acc tcc atc tgg act ggt atc cgc atc 1171 Met Met Val Gly Met Met Gly Thr Ser Ile Trp Thr Gly Ile Arg Ile             345 350 355 tcc aac aca gga aag tac aaa ctc ttc cca cca atc ggc atg gtg gtt 1219 Ser Asn Thr Gly Lys Tyr Lys Leu Phe Pro Pro Ile Gly Met Val Val         360 365 370 acc ttc gtg gca ctg atc ttc ttt gcc cga atg gaa gtg tcc acc acc 1267 Thr Phe Val Ala Leu Ile Phe Phe Ala Arg Met Glu Val Ser Thr Thr     375 380 385 ctg tgg cag atc gga atc tac ctc ttc 1294 Leu Trp Gln Ile Gly Ile Tyr Leu Phe 390 395 <210> 256 <211> 398 <212> PRT <213> Corynebacterium glutamicum <400> 256 Met Thr His Glu Thr Ser Val Pro Gly Pro Ala Asp Ala Gln Val Ala   1 5 10 15 Gly Asp Thr Lys Leu Arg Lys Gly Arg Ala Lys Lys Glu Lys Thr Pro              20 25 30 Ser Ser Met Thr Pro Glu Gln Gln Lys Lys Val Trp Trp Val Leu Ser          35 40 45 Ala Leu Met Val Ala Met Met Met Ala Ser Leu Asp Gln Met Ile Phe      50 55 60 Gly Thr Ala Leu Pro Thr Ile Val Gly Glu Leu Gly Gly Val Asp His  65 70 75 80 Met Met Trp Val Ile Thr Ala Tyr Leu Leu Ala Glu Thr Ile Met Leu                  85 90 95 Pro Ile Tyr Gly Lys Leu Gly Asp Leu Val Gly Arg Lys Gly Leu Phe             100 105 110 Ile Gly Ala Leu Gly Ile Phe Leu Ile Gly Ser Val Ile Gly Gly Leu         115 120 125 Ala Gly Asn Met Thr Trp Leu Ile Val Gly Arg Ala Val Gln Gly Ile     130 135 140 Gly Gly Gly Gly Leu Met Ile Leu Ser Gln Ala Ile Ile Ala Asp Val 145 150 155 160 Val Pro Ala Arg Glu Arg Gly Arg Tyr Met Gly Val Met Gly Gly Val                 165 170 175 Phe Gly Leu Ser Ala Val Leu Gly Pro Leu Leu Gly Gly Trp Phe Thr             180 185 190 Glu Gly Pro Gly Trp Arg Trp Ala Phe Trp Met Asn Ile Pro Leu Gly         195 200 205 Ile Ile Ala Ile Gly Val Ala Ile Tyr Phe Leu Asp Ile Pro Lys Lys     210 215 220 Ser Val Lys Phe Arg Trp Asp Tyr Leu Gly Thr Phe Phe Met Ile Val 225 230 235 240 Ala Ala Thr Ser Leu Ile Leu Phe Thr Thr Trp Gly Gly Ser Gln Tyr                 245 250 255 Glu Trp Ser Asp Pro Ile Ile Ile Gly Leu Ile Ile Thr Thr Ile Val             260 265 270 Ala Ala Ala Leu Leu Val Val Val Glu Leu Arg Ala Lys Asp Pro Leu         275 280 285 Val Pro Met Ser Phe Phe Gln Asn Arg Asn Phe Thr Leu Thr Thr Ile     290 295 300 Ala Gly Leu Ile Leu Gly Ile Ala Met Phe Gly Ile Ile Gly Tyr Leu 305 310 315 320 Pro Thr Tyr Leu Gln Met Val His Gly Ile Asn Ala Thr Glu Ala Gly                 325 330 335 Tyr Met Leu Ile Pro Met Met Val Gly Met Met Gly Thr Ser Ile Trp             340 345 350 Thr Gly Ile Arg Ile Ser Asn Thr Gly Lys Tyr Lys Leu Phe Pro Pro         355 360 365 Ile Gly Met Val Val Thr Phe Val Ala Leu Ile Phe Phe Ala Arg Met     370 375 380 Glu Val Ser Thr Thr Leu Trp Gln Ile Gly Ile Tyr Leu Phe 385 390 395 <210> 257 <211> 1510 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (93) .. (1487) <223> RXA01578 <400> 257 tgacctcccc tcaaccactc actgaaaaga gaacactgat gtcacttcct gttcagccga 60 gtaaaacctc ggccgccaca gtcataccat tg atg atc gcc ctg ctg gtc gcg 113                                     Met Ile Ala Leu Leu Val Ala                                      1 5 gta ttc gcc ttc cag ctc aac gcc tcc atg ctg gcg ccg gca ctg gcc 161 Val Phe Ala Phe Gln Leu Asn Ala Ser Met Leu Ala Pro Ala Leu Ala          10 15 20 acc atg gaa act gaa ctt aat gca aca gct gcc caa atc ggc atg acg 209 Thr Met Glu Thr Glu Leu Asn Ala Thr Ala Ala Gln Ile Gly Met Thr      25 30 35 cag act gct ttc ttc acc gcg gcg gcg ctg ttt tcc ctg ttc ctg cca 257 Gln Thr Ala Phe Phe Thr Ala Ala Ala Leu Phe Ser Leu Phe Leu Pro  40 45 50 55 cgt tgg ggc gat ctg att ggt cgc cgc aaa gtg ctg gtc ggc atg atg 305 Arg Trp Gly Asp Leu Ile Gly Arg Arg Lys Val Leu Val Gly Met Met                  60 65 70 att gtc acc ggt att gga tgt gtt gtc gct gcc ttt gct ccg aat gtg 353 Ile Val Thr Gly Ile Gly Cys Val Val Ala Ala Phe Ala Pro Asn Val              75 80 85 acc atc ctc ttc ctg ggc cgc ctg att caa ggt gtt gct ggc cca acc 401 Thr Ile Leu Phe Leu Gly Arg Leu Ile Gln Gly Val Ala Gly Pro Thr          90 95 100 gtg cca ctg tgt ctg atc att ctg cgc cag cag gta acc aat gaa aag 449 Val Pro Leu Cys Leu Ile Ile Leu Arg Gln Gln Val Thr Asn Glu Lys     105 110 115 caa tat gcg cta ctt ctc gga att gtt acc tct gtc aac ggt ggt atc 497 Gln Tyr Ala Leu Leu Leu Gly Ile Val Thr Ser Ser As As Gly Gly Ile 120 125 130 135 ggc ggc gtg gac gcg ctt gct ggt ggc tgg ttg gct gaa aca ctt ggt 545 Gly Gly Val Asp Ala Leu Ala Gly Gly Trp Leu Ala Glu Thr Leu Gly                 140 145 150 ttc cgt tcc atc ttc tgg gtc atg gct gct ttc tgc gct gtc gct gcc 593 Phe Arg Ser Ile Phe Trp Val Met Ala Ala Phe Cys Ala Val Ala Ala             155 160 165 ctc gca ctg cct ttc agc gtg aag gaa tcc acc gct gaa gaa acc ccg 641 Leu Ala Leu Pro Phe Ser Val Lys Glu Ser Thr Ala Glu Glu Thr Pro         170 175 180 aag atg gac tgg ctt ggt gtg ctg cca ctg gcg gtg tcc att gga tct 689 Lys Met Asp Trp Leu Gly Val Leu Pro Leu Ala Val Ser Ile Gly Ser     185 190 195 ttg ctc atg gct ttc aac gag gcc ggc aaa ctc ggt gcc gcg aac tgg 737 Leu Leu Met Ala Phe Asn Glu Ala Gly Lys Leu Gly Ala Ala Asn Trp 200 205 210 215 att ctg gtg gtt gtg ctg ttc atc atc ggt atc gcc gga gtc atc ttc 785 Ile Leu Val Val Val Leu Phe Ile Ile Gly Ile Ala Gly Val Ile Phe                 220 225 230 ttc tac aac att gaa aag cgc gtt aag cac ccg ctg gtc agt gtt gaa 833 Phe Tyr Asn Ile Glu Lys Arg Val Lys His Pro Leu Val Ser Val Glu             235 240 245 tac ctc ggt caa cga cgc acc tgg gca ttg ctg ctg agc acc ctt ctc 881 Tyr Leu Gly Gln Arg Arg Thr Trp Ala Leu Leu Leu Ser Thr Leu Leu         250 255 260 aca atg acc ggt gta ttc gcc gta atg aat ggt ctg ctg ccc aac ctt 929 Thr Met Thr Gly Val Phe Ala Val Met Asn Gly Leu Leu Pro Asn Leu     265 270 275 gcg cag gat gct gcc aac ggt gcc ggc atg tca gcg agc gtg gtg tcc 977 Ala Gln Asp Ala Ala Asn Gly Ala Gly Met Ser Ala Ser Val Val Ser 280 285 290 295 tgg tgg aca ctt acc cca tat gcg ctg gct ggc ttg gta ttc ggt cca 1025 Trp Trp Thr Leu Thr Pro Tyr Ala Leu Ala Gly Leu Val Phe Gly Pro                 300 305 310 atc gcc gga att ctc gcc gga aaa ttt gga tac aag atc gtc ctg caa 1073 Ile Ala Gly Ile Leu Ala Gly Lys Phe Gly Tyr Lys Ile Val Leu Gln             315 320 325 att ggt atc gct gcc acc atc atc ggc gtt gcc gga gcc acc ttc tta 1121 Ile Gly Ile Ala Ala Thr Ile Ile Gly Val Ala Gly Ala Thr Phe Leu         330 335 340 gtc gga agc acc tcg cat ctc gcg tac ctc ggc atc tcc atc ttc gtg 1169 Val Gly Ser Thr Ser His Leu Ala Tyr Leu Gly Ile Ser Ile Phe Val     345 350 355 ggt att acc tat gca ggt att gcc aac atc atg ctc aac ggc ctg ggc 1217 Gly Ile Thr Tyr Ala Gly Ile Ala Asn Ile Met Leu Asn Gly Leu Gly 360 365 370 375 atc gtg ctc tcc cct gct aac aac caa ggc tat ctg cct ggc atg aac 1265 Ile Val Leu Ser Pro Ala Asn Asn Gln Gly Tyr Leu Pro Gly Met Asn                 380 385 390 gca ggt gcc ttc aac cta ggt gca ggt att tcc ttc gcc atc ctc ttc 1313 Ala Gly Ala Phe Asn Leu Gly Ala Gly Ile Ser Phe Ala Ile Leu Phe             395 400 405 gca gtt tcc acg gca ttc agt gac aac ggc gga gga tac gcc gca ggc 1361 Ala Val Ser Thr Ala Phe Ser Asp Asn Gly Gly Gly Tyr Ala Ala Gly         410 415 420 atg tgg gct ggc gtg atc atc ttg gtc cta gcc ttc ctc tgc tcc ctg 1409 Met Trp Ala Gly Val Ile Ile Leu Val Leu Ala Phe Leu Cys Ser Leu     425 430 435 ctg atc cca cgc cca gaa tca atc acc gat aca gtg gca gcc aaa gtc 1457 Leu Ile Pro Arg Pro Glu Ser Ile Thr Asp Thr Val Ala Ala Lys Val 440 445 450 455 cag gct gaa gaa gcc gct caa gcc gcc agc taaatccaca aactgaacta 1507 Gln Ala Glu Glu Ala Ala Gln Ala Ala Ser                 460 465 agg 1510 <210> 258 <211> 465 <212> PRT <213> Corynebacterium glutamicum <400> 258 Met Ile Ala Leu Leu Val Ala Val Phe Ala Phe Gln Leu Asn Ala Ser   1 5 10 15 Met Leu Ala Pro Ala Leu Ala Thr Met Glu Thr Glu Leu Asn Ala Thr              20 25 30 Ala Ala Gln Ile Gly Met Thr Gln Thr Ala Phe Phe Thr Ala Ala Ala          35 40 45 Leu Phe Ser Leu Phe Leu Pro Arg Trp Gly Asp Leu Ile Gly Arg Arg      50 55 60 Lys Val Leu Val Gly Met Met Ile Val Thr Gly Ile Gly Cys Val Val  65 70 75 80 Ala Ala Phe Ala Pro Asn Val Thr Ile Leu Phe Leu Gly Arg Leu Ile                  85 90 95 Gln Gly Val Ala Gly Pro Thr Val Pro Leu Cys Leu Ile Ile Leu Arg             100 105 110 Gln Gln Val Thr Asn Glu Lys Gln Tyr Ala Leu Leu Leu Gly Ile Val         115 120 125 Thr Ser Val Asn Gly Gly Ile Gly Gly Val Asp Ala Leu Ala Gly Gly     130 135 140 Trp Leu Ala Glu Thr Leu Gly Phe Arg Ser Ile Phe Trp Val Met Ala 145 150 155 160 Ala Phe Cys Ala Val Ala Ala Leu Ala Leu Pro Phe Ser Val Lys Glu                 165 170 175 Ser Thr Ala Glu Glu Thr Pro Lys Met Asp Trp Leu Gly Val Leu Pro             180 185 190 Leu Ala Val Ser Ile Gly Ser Leu Leu Met Ala Phe Asn Glu Ala Gly         195 200 205 Lys Leu Gly Ala Ala Asn Trp Ile Leu Val Val Val Leu Phe Ile Ile     210 215 220 Gly Ile Ala Gly Val Ile Phe Phe Tyr Asn Ile Glu Lys Arg Val Lys 225 230 235 240 His Pro Leu Val Ser Val Glu Tyr Leu Gly Gln Arg Arg Thr Trp Ala                 245 250 255 Leu Leu Leu Ser Thr Leu Leu Thr Met Thr Gly Val Phe Ala Val Met             260 265 270 Asn Gly Leu Leu Pro Asn Leu Ala Gln Asp Ala Ala Asn Gly Ala Gly         275 280 285 Met Ser Ala Ser Val Val Ser Trp Trp Thr Leu Thr Pro Tyr Ala Leu     290 295 300 Ala Gly Leu Val Phe Gly Pro Ile Ala Gly Ile Leu Ala Gly Lys Phe 305 310 315 320 Gly Tyr Lys Ile Val Leu Gln Ile Gly Ile Ala Ala Thr Ile Ile Gly                 325 330 335 Val Ala Gly Ala Thr Phe Leu Val Gly Ser Thr Ser His Leu Ala Tyr             340 345 350 Leu Gly Ile Ser Ile Phe Val Gly Ile Thr Tyr Ala Gly Ile Ala Asn         355 360 365 Ile Met Leu Asn Gly Leu Gly Ile Val Leu Ser Pro Ala Asn Asn Gln     370 375 380 Gly Tyr Leu Pro Gly Met Asn Ala Gly Ala Phe Asn Leu Gly Ala Gly 385 390 395 400 Ile Ser Phe Ala Ile Leu Phe Ala Val Ser Thr Ala Phe Ser Asp Asn                 405 410 415 Gly Gly Gly Tyr Ala Ala Gly Met Trp Ala Gly Val Ile Ile Leu Val             420 425 430 Leu Ala Phe Leu Cys Ser Leu Leu Ile Pro Arg Pro Glu Ser Ile Thr         435 440 445 Asp Thr Val Ala Ala Lys Val Gln Ala Glu Glu Ala Ala Gln Ala Ala     450 455 460 Ser 465 <210> 259 <211> 1470 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1447) <223> RXA02087 <400> 259 aatcggattc atgctgtgtg gtgtgatcag tttgctggct gcggtcgcat ggatcttcgg 60 ccgggagacg ctgccaacgg cgaaagtcga gcaggtataa atg acg cca att gtg 115                                             Met Thr Pro Ile Val                                               1 5 gag tcc agg gcg tgg aaa gct ctg ggc gct tta agt gtt ggg ctg ttt 163 Glu Ser Arg Ala Trp Lys Ala Leu Gly Ala Leu Ser Val Gly Leu Phe                  10 15 20 ctc aca ctg ctt gac caa tcg ttg gtg gct gtc gcg ctg cca aag att 211 Leu Thr Leu Leu Asp Gln Ser Leu Val Ala Val Ala Leu Pro Lys Ile              25 30 35 caa gag gat ttg ggt gcg agc ctg aac caa gcg gtg tgg gtg tca gcg 259 Gln Glu Asp Leu Gly Ala Ser Leu Asn Gln Ala Val Trp Val Ser Ala          40 45 50 gtt tat ttg ctc act ttt gcg gtg cca ctg ttg att act ggg cgc ttg 307 Val Tyr Leu Leu Thr Phe Ala Val Pro Leu Leu Ile Thr Gly Arg Leu      55 60 65 ggt gat cgt tat gga cag cga aat att tat ctt gcc ggc atg gct gtg 355 Gly Asp Arg Tyr Gly Gln Arg Asn Ile Tyr Leu Ala Gly Met Ala Val  70 75 80 85 ttt acc ctc gcg gcg ttg gcc tgt gta ttt gca cca agc atc gaa tgg 403 Phe Thr Leu Ala Ala Leu Ala Cys Val Phe Ala Pro Ser Ile Glu Trp                  90 95 100 ttg att gct gct cgc gcg gtg cag ggc ctg ggc gga tct ctt ctt aat 451 Leu Ile Ala Ala Arg Ala Val Gln Gly Leu Gly Gly Ser Leu Leu Asn             105 110 115 ccg cag ccc ctg agc atc att cac aag att ttc gcg cat gat cgt agg 499 Pro Gln Pro Leu Ser Ile Ile His Lys Ile Phe Ala His Asp Arg Arg         120 125 130 gga gcc gcc acc ggg gtg tgg agt gct gtt gcc tca tca gct gga ctt 547 Gly Ala Ala Thr Gly Val Trp Ser Ala Val Ala Ser Ser Ala Gly Leu     135 140 145 ttc ggg cca gtt atc ggt ggt gtt ctg gtg ggg tgg atc agc tgg cgt 595 Phe Gly Pro Val Ile Gly Gly Val Leu Val Gly Trp Ile Ser Trp Arg 150 155 160 165 gct gtg ttc ttg gtt tat gtg ccg ctc gga ttg atc tcc cta ttt atg 643 Ala Val Phe Leu Val Tyr Val Pro Leu Gly Leu Ile Ser Leu Phe Met                 170 175 180 gtg gcg cgt tat gtg cct aaa ctt ccc acg gga acc tcg aag atc gat 691 Val Ala Arg Tyr Val Pro Lys Leu Pro Thr Gly Thr Ser Lys Ile Asp             185 190 195 tgg ctc tcg ggt gcg gtc tca ctt gtt gct gta ctt ggt gtg gtt ctt 739 Trp Leu Ser Gly Ala Val Ser Leu Val Ala Val Leu Gly Val Val Leu         200 205 210 gcc ttg cag cag ggg cca gaa ctt ggg tgg gga aca ctg att tgg gtg 787 Ala Leu Gln Gln Gly Pro Glu Leu Gly Trp Gly Thr Leu Ile Trp Val     215 220 225 tcc ctt gcc gtt ggt att gct gca gct gtg ctc ttt ata tgg atg caa 835 Ser Leu Ala Val Gly Ile Ala Ala Ala Val Leu Phe Ile Trp Met Gln 230 235 240 245 aca aga tcc aag gcg cca ctg atg ccg ttg agg att ttc aag acg cgc 883 Thr Arg Ser Lys Ala Pro Leu Met Pro Leu Arg Ile Phe Lys Thr Arg                 250 255 260 aac ttc gcg atc ggt gca ttt tcg atc ttc agc ctg ggc ttt acg gtg 931 Asn Phe Ala Ile Gly Ala Phe Ser Ile Phe Ser Leu Gly Phe Thr Val             265 270 275 tat tcc gtt aat ttg ccc atc atg ttg tat ctg caa acg gct cag gga 979 Tyr Ser Val Asn Leu Pro Ile Met Leu Tyr Leu Gln Thr Ala Gln Gly         280 285 290 atg tcg tcg cag ttg gcc ggt ttg atg ttg gtt ccg atg ggc atc atc 1027 Met Ser Ser Gln Leu Ala Gly Leu Met Leu Val Pro Met Gly Ile Ile     295 300 305 tct gtg gtg atg tca cca gta att gga cga ttg gtg gat cgc ctg gca 1075 Ser Val Val Met Ser Pro Val Ile Gly Arg Leu Val Asp Arg Leu Ala 310 315 320 325 cca gga atg atc tc aag atc gga ttc ggc gcg ctg att ttc tcg atg 1123 Pro Gly Met Ile Ser Lys Ile Gly Phe Gly Ala Leu Ile Phe Ser Met                 330 335 340 gcg ttg atg gct gtc ttt atg atc gcc aac cta tcg ccg tgg tgg cta 1171 Ala Leu Met Ala Val Phe Met Ile Ala Asn Leu Ser Pro Trp Trp Leu             345 350 355 ctc atc ccg att att ttg ttc ggt agc tcc aac gcg atg agt ttt gca 1219 Leu Ile Pro Ile Ile Leu Phe Gly Ser Ser Asn Ala Met Ser Phe Ala         360 365 370 ccg aac tct gtg att gct ctg cgt gat gtt ccg cag gat tta gtg ggc 1267 Pro Asn Ser Val Ile Ala Leu Arg Asp Val Pro Gln Asp Leu Val Gly     375 380 385 tct gct tct ggt ttt tac aac acc tca cgc cag gtg ggc gct gtt ttg 1315 Ser Ala Ser Gly Phe Tyr Asn Thr Ser Arg Gln Val Gly Ala Val Leu 390 395 400 405 ggc gcc gct acc ttg ggc gct gtg atg caa ata gga gtg ggc acg gtg 1363 Gly Ala Ala Thr Leu Gly Ala Val Met Gln Ile Gly Val Gly Thr Val                 410 415 420 tcc ttc ggt gtt gcc atg ggt gcg gca atc ctg gtg aca ctc gtg ccc 1411 Ser Phe Gly Val Ala Met Gly Ala Ala Ile Leu Val Thr Leu Val Pro             425 430 435 tta atc ttt ggg ttc cta gcg gta acc caa ttt aga tagttgctcc 1457 Leu Ile Phe Gly Phe Leu Ala Val Thr Gln Phe Arg         440 445 gattttctca cga 1470 <210> 260 <211> 449 <212> PRT <213> Corynebacterium glutamicum <400> 260 Met Thr Pro Ile Val Glu Ser Arg Ala Trp Lys Ala Leu Gly Ala Leu   1 5 10 15 Ser Val Gly Leu Phe Leu Thr Leu Leu Asp Gln Ser Leu Val Ala Val              20 25 30 Ala Leu Pro Lys Ile Gln Glu Asp Leu Gly Ala Ser Leu Asn Gln Ala          35 40 45 Val Trp Val Ser Ala Val Tyr Leu Leu Thr Phe Ala Val Pro Leu Leu      50 55 60 Ile Thr Gly Arg Leu Gly Asp Arg Tyr Gly Gln Arg Asn Ile Tyr Leu  65 70 75 80 Ala Gly Met Ala Val Phe Thr Leu Ala Ala Leu Ala Cys Val Phe Ala                  85 90 95 Pro Ser Ile Glu Trp Leu Ile Ala Ala Arg Ala Val Gln Gly Leu Gly             100 105 110 Gly Ser Leu Leu Asn Pro Gln Pro Leu Ser Ile Ile His Lys Ile Phe         115 120 125 Ala His Asp Arg Arg Gly Ala Ala Thr Gly Val Trp Ser Ala Val Ala     130 135 140 Ser Ser Ala Gly Leu Phe Gly Pro Val Ile Gly Gly Val Leu Val Gly 145 150 155 160 Trp Ile Ser Trp Arg Ala Val Phe Leu Val Tyr Val Pro Leu Gly Leu                 165 170 175 Ile Ser Leu Phe Met Val Ala Arg Tyr Val Pro Lys Leu Pro Thr Gly             180 185 190 Thr Ser Lys Ile Asp Trp Leu Ser Gly Ala Val Ser Leu Val Ala Val         195 200 205 Leu Gly Val Val Leu Ala Leu Gln Gln Gly Pro Glu Leu Gly Trp Gly     210 215 220 Thr Leu Ile Trp Val Ser Leu Ala Val Gly Ile Ala Ala Ala Val Leu 225 230 235 240 Phe Ile Trp Met Gln Thr Arg Ser Lys Ala Pro Leu Met Pro Leu Arg                 245 250 255 Ile Phe Lys Thr Arg Asn Phe Ala Ile Gly Ala Phe Ser Ile Phe Ser             260 265 270 Leu Gly Phe Thr Val Tyr Ser Val Asn Leu Pro Ile Met Leu Tyr Leu         275 280 285 Gln Thr Ala Gln Gly Met Ser Ser Gln Leu Ala Gly Leu Met Leu Val     290 295 300 Pro Met Gly Ile Ile Ser Val Val Met Ser Pro Val Ile Gly Arg Leu 305 310 315 320 Val Asp Arg Leu Ala Pro Gly Met Ile Ser Lys Ile Gly Phe Gly Ala                 325 330 335 Leu Ile Phe Ser Met Ala Leu Met Ala Val Phe Met Ile Ala Asn Leu             340 345 350 Ser Pro Trp Trp Leu Leu Ile Pro Ile Ile Leu Phe Gly Ser Ser Asn         355 360 365 Ala Met Ser Phe Ala Pro Asn Ser Val Ile Ala Leu Arg Asp Val Pro     370 375 380 Gln Asp Leu Val Gly Ser Ala Ser Gly Phe Tyr Asn Thr Ser Arg Gln 385 390 395 400 Val Gly Ala Val Leu Gly Ala Ala Thr Leu Gly Ala Val Met Gln Ile                 405 410 415 Gly Val Gly Thr Val Ser Phe Gly Val Ala Met Gly Ala Ala Ile Leu             420 425 430 Val Thr Leu Val Pro Leu Ile Phe Gly Phe Leu Ala Val Thr Gln Phe         435 440 445 Arg      <210> 261 <211> 1338 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (101) .. (1315) <223> RXA02088 <400> 261 agagcaccca agcgacattg ttcgataata tgttctatta ttggtattaa tactggtcag 60 ctagatataa aaggggcgca gggctagcct tgggtgtgaa atg tta act caa aaa 115                                             Met Leu Thr Gln Lys                                               1 5 ata gaa tta gag gct aaa cca aaa atc cca gag gag atc tgg gtg ctg 163 Ile Glu Leu Glu Ala Lys Pro Lys Ile Pro Glu Glu Ile Trp Val Leu                  10 15 20 gtt gtg gct gcg ttt att att gcg ctg ggc tat ggc ctg att gcg ccg 211 Val Val Ala Ala Phe Ile Ile Ala Leu Gly Tyr Gly Leu Ile Ala Pro              25 30 35 att ttg cca cag ttt gtg gtc ggt ttt gat gta agt ttt gca gct gcc 259 Ile Leu Pro Gln Phe Val Val Gly Phe Asp Val Ser Phe Ala Ala Ala          40 45 50 agt gcg gtg gtg tcc atc ttt gcg ggc gcc cgg ttg ttg ttt gcg ccg 307 Ser Ala Val Val Ser Ile Phe Ala Gly Ala Arg Leu Leu Phe Ala Pro      55 60 65 atg tcg ggg agt ttg atc gat aag atc ggt tcc cgt cgt gtg tat ctc 355 Met Ser Gly Ser Leu Ile Asp Lys Ile Gly Ser Arg Arg Val Tyr Leu  70 75 80 85 act ggt tta ctc acc gtg gct atc acc acg ggg ctt gtt gcg ttg gcg 403 Thr Gly Leu Leu Thr Val Ala Ile Thr Thr Gly Leu Val Ala Leu Ala                  90 95 100 cag gaa tac tgg cag att ctg ctg ctt cgt ggc atc gca ggt att ggt 451 Gln Glu Tyr Trp Gln Ile Leu Leu Leu Arg Gly Ile Ala Gly Ile Gly             105 110 115 tcc acc atg ttt acg gtc tct gcc atg ggc ctg atc gtg aag atg gcg 499 Ser Thr Met Phe Thr Val Ser Ala Met Gly Leu Ile Val Lys Met Ala         120 125 130 ccg gtg gag atc cgc ggg cgg tgt tcg tcg gta tat gcc agt tcg ttc 547 Pro Val Glu Ile Arg Gly Arg Cys Ser Ser Val Tyr Ala Ser Ser Phe     135 140 145 ctg ttt ggc aat att att ggc ccg gtt gtg ggt gct gcg atg tcc ggt 595 Leu Phe Gly Asn Ile Gly Pro Val Val Gly Ala Ala Met Ser Gly 150 155 160 165 ttg ggc atg cgg tgg ccg ttc gcg att tat ggt gct tcc gtt ggc tta 643 Leu Gly Met Arg Trp Pro Phe Ala Ile Tyr Gly Ala Ser Val Gly Leu                 170 175 180 gct gca ctt gtt gtg tgg tgg cgg atg ccg aaa acc aac gat tca ctt 691 Ala Ala Leu Val Val Trp Trp Arg Met Pro Lys Thr Asn Asp Ser Leu             185 190 195 cgg aag gct gat agc aat agt gtg ccg gcg ttg cgc ttt gct gag gca 739 Arg Lys Ala Asp Ser Asn Ser Val Pro Ala Leu Arg Phe Ala Glu Ala         200 205 210 att aag gat tct gcc tac cgc tcg gcg ttg ttt agt gct ttt gcc aat 787 Ile Lys Asp Ser Ala Tyr Arg Ser Ala Leu Phe Ser Ala Phe Ala Asn     215 220 225 ggt tgg tcg aac ttt ggt gtg cgt gtg gcg gtt ctt cca ctg ttt gcc 835 Gly Trp Ser Asn Phe Gly Val Arg Val Ala Val Leu Pro Leu Phe Ala 230 235 240 245 gca gct gca ttt agc aat ggc gga gct att gcg ggt ttt gcc atg gct 883 Ala Ala Ala Phe Ser Asn Gly Gly Ala Ile Ala Gly Phe Ala Met Ala                 250 255 260 gcg ttc gcc gct gga aat gct ttg tgt ctg caa ttc gcg ggc gat ctc 931 Ala Phe Ala Ala Gly Asn Ala Leu Cys Leu Gln Phe Ala Gly Asp Leu             265 270 275 tca gat cgc att ggc cgt aaa ccg atg att att tcc ggg ctg atc gtc 979 Ser Asp Arg Ile Gly Arg Lys Pro Met Ile Ile Ser Gly Leu Ile Val         280 285 290 aat gca gtg ttc acg gca atg atc gga ttc ggc aca gaa gtg tgg atc 1027 Asn Ala Val Phe Thr Ala Met Ile Gly Phe Gly Thr Glu Val Trp Ile     295 300 305 ctg atc acg gta tct gcg ttg gca ggt gct ggt gcg ggc ttg ctt aat 1075 Leu Ile Thr Val Ser Ala Leu Ala Gly Ala Gly Ala Gly Leu Leu Asn 310 315 320 325 cca agt cag cag gcg gtg ctc gct gat gtt ata gat tcc cgc ccc ggc 1123 Pro Ser Gln Gln Ala Val Leu Ala Asp Val Ile Asp Ser Arg Pro Gly                 330 335 340 gga aaa gtc tta gcg aat ttc caa atg gcg cag gat ttc ggt gcg att 1171 Gly Lys Val Leu Ala Asn Phe Gln Met Ala Gln Asp Phe Gly Ala Ile             345 350 355 gtt ggc ccg att ctc gta ggc atg atc gca gaa cag gca ggc ttc caa 1219 Val Gly Pro Ile Leu Val Gly Met Ile Ala Glu Gln Ala Gly Phe Gln         360 365 370 atc gga ttc atg ctg tgt ggt gtg atc agt ttg ctg gct gcg gtc gca 1267 Ile Gly Phe Met Leu Cys Gly Val Ile Ser Leu Leu Ala Ala Val Ala     375 380 385 tgg atc ttc ggc cgg gag acg ctg cca acg gcg aaa gtc gag cag gta 1315 Trp Ile Phe Gly Arg Glu Thr Leu Pro Thr Ala Lys Val Glu Gln Val 390 395 400 405 taaatgacgc caattgtgga gtc 1338 <210> 262 <211> 405 <212> PRT <213> Corynebacterium glutamicum <400> 262 Met Leu Thr Gln Lys Ile Glu Leu Glu Ala Lys Pro Lys Ile Pro Glu   1 5 10 15 Glu Ile Trp Val Leu Val Val Ala Ala Phe Ile Ile Ala Leu Gly Tyr              20 25 30 Gly Leu Ile Ala Pro Ile Leu Pro Gln Phe Val Val Gly Phe Asp Val          35 40 45 Ser Phe Ala Ala Ala Ser Ala Val Val Ser Ile Phe Ala Gly Ala Arg      50 55 60 Leu Leu Phe Ala Pro Met Ser Gly Ser Leu Ile Asp Lys Ile Gly Ser  65 70 75 80 Arg Arg Val Tyr Leu Thr Gly Leu Leu Thr Val Ala Ile Thr Thr Gly                  85 90 95 Leu Val Ala Leu Ala Gln Glu Tyr Trp Gln Ile Leu Leu Leu Arg Gly             100 105 110 Ile Ala Gly Ile Gly Ser Thr Met Phe Thr Val Ser Ala Met Gly Leu         115 120 125 Ile Val Lys Met Ala Pro Val Glu Ile Arg Gly Arg Cys Ser Ser Val     130 135 140 Tyr Ala Ser Ser Phe Leu Phe Gly Asn Ile Ile Gly Pro Val Val Gly 145 150 155 160 Ala Ala Met Ser Gly Leu Gly Met Arg Trp Pro Phe Ala Ile Tyr Gly                 165 170 175 Ala Ser Val Gly Leu Ala Ala Leu Val Val Trp Trp Arg Met Pro Lys             180 185 190 Thr Asn Asp Ser Leu Arg Lys Ala Asp Ser Asn Ser Val Pro Ala Leu         195 200 205 Arg Phe Ala Glu Ala Ile Lys Asp Ser Ala Tyr Arg Ser Ala Leu Phe     210 215 220 Ser Ala Phe Ala Asn Gly Trp Ser Asn Phe Gly Val Arg Val Ala Val 225 230 235 240 Leu Pro Leu Phe Ala Ala Ala Ala Phe Ser Asn Gly Gly Ala Ile Ala                 245 250 255 Gly Phe Ala Met Ala Ala Phe Ala Ala Gly Asn Ala Leu Cys Leu Gln             260 265 270 Phe Ala Gly Asp Leu Ser Asp Arg Ile Gly Arg Lys Pro Met Ile Ile         275 280 285 Ser Gly Leu Ile Val Asn Ala Val Phe Thr Ala Met Ile Gly Phe Gly     290 295 300 Thr Glu Val Trp Ile Leu Ile Thr Val Ser Ala Leu Ala Gly Ala Gly 305 310 315 320 Ala Gly Leu Leu Asn Pro Ser Gln Gln Ala Val Leu Ala Asp Val Ile                 325 330 335 Asp Ser Arg Pro Gly Gly Lys Val Leu Ala Asn Phe Gln Met Ala Gln             340 345 350 Asp Phe Gly Ala Ile Val Gly Pro Ile Leu Val Gly Met Ile Ala Glu         355 360 365 Gln Ala Gly Phe Gln Ile Gly Phe Met Leu Cys Gly Val Ile Ser Leu     370 375 380 Leu Ala Ala Val Ala Trp Ile Phe Gly Arg Glu Thr Leu Pro Thr Ala 385 390 395 400 Lys Val Glu Gln Val                 405 <210> 263 <211> 1239 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) <223> RXA00764 <400> 263 tactgcgtcg gatccgctga tgcttgcaga atcggacagt gatgggccgt ctgcgcctgc 60 acctgggacg actggattat taggggtgga attttcgctc atg aca ctc aag act 115                                             Met Thr Leu Lys Thr                                               1 5 agc gtt ttg gca cta ctc tta gat aac gtg cat gtt ctt ctg att gcg 163 Ser Val Leu Ala Leu Leu Leu Asp Asn Val His Val Leu Leu Ile Ala                  10 15 20 aat cct gag tcg acc acg cag acg cag aaa ctt ttc cgt cgt gtg gtg 211 Asn Pro Glu Ser Thr Thr Gln Thr Gln Lys Leu Phe Arg Arg Val Val              25 30 35 cct gcg ttg atg gcg ctt gat ggt gtg tcg ctt gaa gcg agg ttt acg 259 Pro Ala Leu Met Ala Leu Asp Gly Val Ser Leu Glu Ala Arg Phe Thr          40 45 50 cac tat gga ggc cat gcg gag gaa atg gtt gcg ggt ttg acg gtg gat 307 His Tyr Gly Gly His Ala Glu Glu Met Val Ala Gly Leu Thr Val Asp      55 60 65 gat ttt gat gtg att atc ccc gcg ggt ggg gac ggc acc gtc aac gaa 355 Asp Phe Asp Val Ile Ile Pro Ala Gly Gly Asp Gly Thr Val Asn Glu  70 75 80 85 gtg ata aat ggg tta ctt ggg tcg gcg gaa ggt gat ttt aga aac ctt 403 Val Ile Asn Gly Leu Leu Gly Ser Ala Glu Gly Asp Phe Arg Asn Leu                  90 95 100 gag gat ttg ccg gcg att gcg gtg ttg cca acg ggg tct gcc aat gtg 451 Glu Asp Leu Pro Ala Ile Ala Val Leu Pro Thr Gly Ser Ala Asn Val             105 110 115 ttt gcc cgt gcg ctt ggt tac ccc act gac ccg tat gct gcc gct gat 499 Phe Ala Arg Ala Leu Gly Tyr Pro Thr Asp Pro Tyr Ala Ala Ala Asp         120 125 130 gcc ctg gtg gag ttg att cgg aag aac cac acc aga act atc acc ttg 547 Ala Leu Val Glu Leu Ile Arg Lys Asn His Thr Arg Thr Ile Thr Leu     135 140 145 ggt acg tgg aag ggt gat gat cag ggg act cgt tgg ttc gcg gtt aat 595 Gly Thr Trp Lys Gly Asp Asp Gln Gly Thr Arg Trp Phe Ala Val Asn 150 155 160 165 gct ggg ttt ggt att gat gcg gat gtt att gcc agg gtc gaa cgg gcg 643 Ala Gly Phe Gly Ile Asp Ala Asp Val Ile Ala Arg Val Glu Arg Ala                 170 175 180 aga tct ttc ggc ttt gcg gct tca ccg ttg ttg tat ctg cag gtg agt 691 Arg Ser Phe Gly Phe Ala Ala Ser Pro Leu Leu Tyr Leu Gln Val Ser             185 190 195 ctt cgg gcg tgg gtg aaa act cag att aag cca ccg aaa att acc gtg 739 Leu Arg Ala Trp Val Lys Thr Gln Ile Lys Pro Pro Lys Ile Thr Val         200 205 210 gag gcg gtg gac agc aaa ggg cac aaa ttg caa aaa gag gaa gtg cca 787 Glu Ala Val Asp Ser Lys Gly His Lys Leu Gln Lys Glu Glu Val Pro     215 220 225 atg ctg ctt gcc tcg aat acc aat ccg tgg act ttt ttg ggt ccg ctt 835 Met Leu Leu Ala Ser Asn Thr Asn Pro Trp Thr Phe Leu Gly Pro Leu 230 235 240 245 cct gtg gtg aca aat ccg cag aat tct ttt gac aca ggt ctg ggg ctt 883 Pro Val Val Thr Asn Pro Gln Asn Ser Phe Asp Thr Gly Leu Gly Leu                 250 255 260 ttt ggc ttg act agt gtg cga gga ttc ggg gga gtg gca gcg atg atg 931 Phe Gly Leu Thr Ser Val Arg Gly Phe Gly Gly Val Ala Ala Met Met             265 270 275 cac ctg att ggc gtg ggg cat ggt cgg aag ttg gag aag ttg atc gct 979 His Leu Ile Gly Val Gly His Gly Arg Lys Leu Glu Lys Leu Ile Ala         280 285 290 aag cgc acc att gct ttt gat gat gcg gag aaa gta acg ctc acg tgc 1027 Lys Arg Thr Ile Ala Phe Asp Asp Ala Glu Lys Val Thr Leu Thr Cys     295 300 305 gac agc gat cag cgt ttc caa gtt gat ggt gag tat gaa ggc aaa cca 1075 Asp Ser Asp Gln Arg Phe Gln Val Asp Gly Glu Tyr Glu Gly Lys Pro 310 315 320 325 aca aag gtg gtg ttg gaa tca atc act gat gcg gtg cga gtg tat gcg 1123 Thr Lys Val Val Leu Glu Ser Ile Thr Asp Ala Val Arg Val Tyr Ala                 330 335 340 ccg aag acg cat ccg aca ccg ccg atc atg aat tgg gct gtc cat ttg 1171 Pro Lys Thr His Pro Thr Pro Pro Ile Met Asn Trp Ala Val His Leu             345 350 355 ttt aag cac gtc cgt gat ttc ctc cgg gtg cgc acg ttt ggc atc 1216 Phe Lys His Val Arg Asp Phe Leu Arg Val Arg Thr Phe Gly Ile         360 365 370 taggattcat cggagttttc ttc 1239 <210> 264 <211> 372 <212> PRT <213> Corynebacterium glutamicum <400> 264 Met Thr Leu Lys Thr Ser Val Leu Ala Leu Leu Leu Asp Asn Val His   1 5 10 15 Val Leu Leu Ile Ala Asn Pro Glu Ser Thr Thr Gln Thr Gln Lys Leu              20 25 30 Phe Arg Arg Val Val Pro Ala Leu Met Ala Leu Asp Gly Val Ser Leu          35 40 45 Glu Ala Arg Phe Thr His Tyr Gly Gly His Ala Glu Glu Met Val Ala      50 55 60 Gly Leu Thr Val Asp Asp Phe Asp Val Ile Ile Pro Ala Gly Gly Asp  65 70 75 80 Gly Thr Val Asn Glu Val Ile Asn Gly Leu Leu Gly Ser Ala Glu Gly                  85 90 95 Asp Phe Arg Asn Leu Glu Asp Leu Pro Ala Ile Ala Val Leu Pro Thr             100 105 110 Gly Ser Ala Asn Val Phe Ala Arg Ala Leu Gly Tyr Pro Thr Asp Pro         115 120 125 Tyr Ala Ala Ala Asp Ala Leu Val Glu Leu Ile Arg Lys Asn His Thr     130 135 140 Arg Thr Ile Thr Leu Gly Thr Trp Lys Gly Asp Asp Gln Gly Thr Arg 145 150 155 160 Trp Phe Ala Val Asn Ala Gly Phe Gly Ile Asp Ala Asp Val Ile Ala                 165 170 175 Arg Val Glu Arg Ala Arg Ser Phe Gly Phe Ala Ala Ser Pro Leu Leu             180 185 190 Tyr Leu Gln Val Ser Leu Arg Ala Trp Val Lys Thr Gln Ile Lys Pro         195 200 205 Pro Lys Ile Thr Val Glu Ala Val Asp Ser Lys Gly His Lys Leu Gln     210 215 220 Lys Glu Glu Val Pro Met Leu Leu Ala Ser Asn Thr Asn Pro Trp Thr 225 230 235 240 Phe Leu Gly Pro Leu Pro Val Val Thr Asn Pro Gln Asn Ser Phe Asp                 245 250 255 Thr Gly Leu Gly Leu Phe Gly Leu Thr Ser Val Arg Gly Phe Gly Gly             260 265 270 Val Ala Ala Met Met His Leu Ile Gly Val Gly His Gly Arg Lys Leu         275 280 285 Glu Lys Leu Ile Ala Lys Arg Thr Ile Ala Phe Asp Asp Ala Glu Lys     290 295 300 Val Thr Leu Thr Cys Asp Ser Asp Gln Arg Phe Gln Val Asp Gly Glu 305 310 315 320 Tyr Glu Gly Lys Pro Thr Lys Val Val Leu Glu Ser Ile Thr Asp Ala                 325 330 335 Val Arg Val Tyr Ala Pro Lys Thr His Pro Thr Pro Pro Ile Met Asn             340 345 350 Trp Ala Val His Leu Phe Lys His Val Arg Asp Phe Leu Arg Val Arg         355 360 365 Thr Phe Gly Ile     370 <210> 265 <211> 271 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (271) <223> RXN03125 <400> 265 tgacaccggc gcgacgtatg gcattactgg cgtcacccca atttacgatg acatctctgc 60 tcgcctcggc gacgtcctgg ttccttacgt tctgatcgtt ttg gtt cta gcg ttc 115                                             Leu Val Leu Ala Phe                                               1 5 ctc gtg ctg ttg ctc gtg ttc cgg tcc att tgg gtc cca ttg atc gcg 163 Leu Val Leu Leu Leu Val Phe Arg Ser Ile Trp Val Pro Leu Ile Ala                  10 15 20 gct ctg ggc ttt ggc ttg tca gtt ctg gct acc ttt ggt gct acc gtg 211 Ala Leu Gly Phe Gly Leu Ser Val Leu Ala Thr Phe Gly Ala Thr Val              25 30 35 gcg atc ttc caa gaa ggt gct ttc ggc atc atc gac gat cct cag cca 259 Ala Ile Phe Gln Glu Gly Ala Phe Gly Ile Ile Asp Asp Pro Gln Pro          40 45 50 ctg ctg tgc ttc 271 Leu Leu Cys Phe      55 <210> 266 <211> 57 <212> PRT <213> Corynebacterium glutamicum <400> 266 Leu Val Leu Ala Phe Leu Val Leu Leu Leu Val Phe Arg Ser Ile Trp   1 5 10 15 Val Pro Leu Ile Ala Ala Leu Gly Phe Gly Leu Ser Val Leu Ala Thr              20 25 30 Phe Gly Ala Thr Val Ala Ile Phe Gln Glu Gly Ala Phe Gly Ile Ile          35 40 45 Asp Asp Pro Gln Pro Leu Leu Cys Phe      50 55 <210> 267 <211> 1443 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) .. (1420) <223> RXN01553 <400> 267 atgatgatgt cctcagcaag tccaagcgcc aagccatgct ggaaacaatt ctcgagctga 60 taccaagcca gacttaaatt tctaccttaa agtcttgagc atg act gtt cag gaa 115                                             Met thr val gln glu                                               1 5 ttc gac cgc gcg acc aaa ccc aca cca aaa ccc cca att gtt tct tgg 163 Phe Asp Arg Ala Thr Lys Pro Thr Pro Lys Pro Pro Ile Val Ser Trp                  10 15 20 gcg ttt tgg gat tgg ggt tcc gcc tct ttc aac gcg gtc ctc gtg acc 211 Ala Phe Trp Asp Trp Gly Ser Ala Ser Phe Asn Ala Val Leu Val Thr              25 30 35 ttt att ttc tcg gtc tat ctc act gat tca gtc ggc gcc acc ctc ccc 259 Phe Ile Phe Ser Val Tyr Leu Thr Asp Ser Val Gly Ala Thr Leu Pro          40 45 50 gag ggt tcc aac gcc aca tca ctg tat tcg atg gcg gtc gcc atc gct 307 Glu Gly Ser Asn Ala Thr Ser Leu Tyr Ser Met Ala Val Ala Ile Ala      55 60 65 ggc gtc att gtt gcg gtt gtt gcc cca gtc atg ggc agg cga tca gat 355 Gly Val Ile Val Ala Val Val Ala Pro Val Met Gly Arg Arg Ser Asp  70 75 80 85 atc aag ggc act cgc cgc agg tca ctg cgc atg tgg aca ctt gtc acc 403 Ile Lys Gly Thr Arg Arg Arg Ser Leu Arg Met Trp Thr Leu Val Thr                  90 95 100 gtg ttc ttg atg ttt tgt ctc ttt aca gta aag aac act gat ccc aca 451 Val Phe Leu Met Phe Cys Leu Phe Thr Val Lys Asn Thr Asp Pro Thr             105 110 115 ttt ttc tgg ttt ggt gta gcc atc atg gcg atc gcc aac atc acc ttt 499 Phe Phe Trp Phe Gly Val Ala Ile Met Ala Ile Ala Asn Ile Thr Phe         120 125 130 gag ttc gct gaa gtt cag tac tat gcg cag ctc tcc caa atc tcg acc 547 Glu Phe Ala Glu Val Gln Tyr Tyr Ala Gln Leu Ser Gln Ile Ser Thr     135 140 145 cgc gaa aac gtg ggc cga gtt tct ggt ttc ggc tgg tcc atg ggt tac 595 Arg Glu Asn Val Gly Arg Val Ser Gly Phe Gly Trp Ser Met Gly Tyr 150 155 160 165 ttc ggt ggc atc gtt cta ctg ctt gtt tgt tac cta ggt ttt gtt gcc 643 Phe Gly Gly Ile Val Leu Leu Leu Val Cys Tyr Leu Gly Phe Val Ala                 170 175 180 ggt gat ggc gat acc cgc gga ttc cta aac ctg ccc atc gaa gac ggc 691 Gly Asp Gly Asp Thr Arg Gly Phe Leu Asn Leu Pro Ile Glu Asp Gly             185 190 195 atg aat atc cgc ctc gtc gca gtg ctt gca gcc gtt tgg ttc ttg gtc 739 Met Asn Ile Arg Leu Val Ala Val Leu Ala Ala Val Trp Phe Leu Val         200 205 210 tct gcg att ccg gca ctt ctt cga gtc cca gaa att gag gca cag gta 787 Ser Ala Ile Pro Ala Leu Leu Arg Val Pro Glu Ile Glu Ala Gln Val     215 220 225 gct gcc gaa gac cac ccc aaa ggc ctc ata gct gcc tac aag gat ctc 835 Ala Ala Glu Asp His Pro Lys Gly Leu Ile Ala Ala Tyr Lys Asp Leu 230 235 240 245 ttt ggg cag atc gct gag ctg tgg aaa caa gac cgc aac tcc gtg tat 883 Phe Gly Gln Ile Ala Glu Leu Trp Lys Gln Asp Arg Asn Ser Val Tyr                 250 255 260 ttc ctc atc gca gca act gtt ttc cgt gac gga ctc gcc gga gta ttt 931 Phe Leu Ile Ala Ala Thr Val Phe Arg Asp Gly Leu Ala Gly Val Phe             265 270 275 acc ttc ggt gcc atc ctt gcg gtc tct gtg tac gga cta tct gcc ggt 979 Thr Phe Gly Ala Ile Leu Ala Val Ser Val Tyr Gly Leu Ser Ala Gly         280 285 290 gat gtc ctc ctc ttc ggt gtc gca gcc aac gtg gtc tct gcg ttg gga 1027 Asp Val Leu Leu Phe Gly Val Ala Ala Asn Val Val Ser Ala Leu Gly     295 300 305 gca ctc ctc gga gga ttc cta gac gat cgc gtc ggg cca aaa ccc atc 1075 Ala Leu Leu Gly Gly Phe Leu Asp Asp Arg Val Gly Pro Lys Pro Ile 310 315 320 325 atc ttg att tct ctt gcc atc atg atc gcc gat gct gca att ctc ttc 1123 Ile Leu Ile Ser Leu Ala Ile Met Ile Ala Asp Ala Ala Ile Leu Phe                 330 335 340 ttc gtt gaa ggc ccc act aat ttc tgg atc ttc gga tta atc ctc tgt 1171 Phe Val Glu Gly Pro Thr Asn Phe Trp Ile Phe Gly Leu Ile Leu Cys             345 350 355 gcg ttt gtg gga cct gca cag tca gcg tcg aga agc tat tta aca cgt 1219 Ala Phe Val Gly Pro Ala Gln Ser Ala Ser Arg Ser Tyr Leu Thr Arg         360 365 370 ctt tcc cca gat ggc cag gaa ggc cag ctc ttc ggc ctt tat gcc act 1267 Leu Ser Pro Asp Gly Gln Glu Gly Gln Leu Phe Gly Leu Tyr Ala Thr     375 380 385 acc ggc cgt gcc gtg agt tgg atg gtg ccg tcg ctg ttt ggt gta ttt 1315 Thr Gly Arg Ala Val Ser Trp Met Val Pro Ser Leu Phe Gly Val Phe 390 395 400 405 gtg ggg ctc acc ggc gat gac cgc act ggt att ttg gcc atc gcg ctg 1363 Val Gly Leu Thr Gly Asp Asp Arg Thr Gly Ile Leu Ala Ile Ala Leu                 410 415 420 att ctg cta ttc ggt att gtg ctg ctg agc atg gtg aag cca ccg cac 1411 Ile Leu Leu Phe Gly Ile Val Leu Leu Ser Met Val Lys Pro Pro His             425 430 435 aag gtg aag tagacaaagc gcccacaagg att 1443 Lys Val Lys         440 <210> 268 <211> 440 <212> PRT <213> Corynebacterium glutamicum <400> 268 Met Thr Val Gln Glu Phe Asp Arg Ala Thr Lys Pro Thr Pro Lys Pro   1 5 10 15 Pro Ile Val Ser Trp Ala Phe Trp Asp Trp Gly Ser Ala Ser Phe Asn              20 25 30 Ala Val Leu Val Thr Phe Ile Phe Ser Val Tyr Leu Thr Asp Ser Val          35 40 45 Gly Ala Thr Leu Pro Glu Gly Ser Asn Ala Thr Ser Leu Tyr Ser Met      50 55 60 Ala Val Ala Ile Ala Gly Val Ile Val Ala Val Val Ala Pro Val Met  65 70 75 80 Gly Arg Arg Ser Asp Ile Lys Gly Thr Arg Arg Arg Ser Leu Arg Met                  85 90 95 Trp Thr Leu Val Thr Val Phe Leu Met Phe Cys Leu Phe Thr Val Lys             100 105 110 Asn Thr Asp Pro Thr Phe Phe Trp Phe Gly Val Ala Ile Met Ala Ile         115 120 125 Ala Asn Ile Thr Phe Glu Phe Ala Glu Val Gln Tyr Tyr Ala Gln Leu     130 135 140 Ser Gln Ile Ser Thr Arg Glu Asn Val Gly Arg Val Ser Gly Phe Gly 145 150 155 160 Trp Ser Met Gly Tyr Phe Gly Gly Ile Val Leu Leu Leu Val Cys Tyr                 165 170 175 Leu Gly Phe Val Ala Gly Asp Gly Asp Thr Arg Gly Phe Leu Asn Leu             180 185 190 Pro Ile Glu Asp Gly Met Asn Ile Arg Leu Val Ala Val Leu Ala Ala         195 200 205 Val Trp Phe Leu Val Ser Ala Ile Pro Ala Leu Leu Arg Val Pro Glu     210 215 220 Ile Glu Ala Gln Val Ala Ala Glu Asp His Pro Lys Gly Leu Ile Ala 225 230 235 240 Ala Tyr Lys Asp Leu Phe Gly Gln Ile Ala Glu Leu Trp Lys Gln Asp                 245 250 255 Arg Asn Ser Val Tyr Phe Leu Ile Ala Ala Thr Val Phe Arg Asp Gly             260 265 270 Leu Ala Gly Val Phe Thr Phe Gly Ala Ile Leu Ala Val Ser Val Tyr         275 280 285 Gly Leu Ser Ala Gly Asp Val Leu Leu Phe Gly Val Ala Ala Asn Val     290 295 300 Val Ser Ala Leu Gly Ala Leu Leu Gly Gly Phe Leu Asp Asp Arg Val 305 310 315 320 Gly Pro Lys Pro Ile Ile Leu Ile Ser Leu Ala Ile Met Ile Ala Asp                 325 330 335 Ala Ala Ile Leu Phe Phe Val Glu Gly Pro Thr Asn Phe Trp Ile Phe             340 345 350 Gly Leu Ile Leu Cys Ala Phe Val Gly Pro Ala Gln Ser Ala Ser Arg         355 360 365 Ser Tyr Leu Thr Arg Leu Ser Pro Asp Gly Gln Glu Gly Gln Leu Phe     370 375 380 Gly Leu Tyr Ala Thr Thr Gly Arg Ala Val Ser Trp Met Val Pro Ser 385 390 395 400 Leu Phe Gly Val Phe Val Gly Leu Thr Gly Asp Asp Arg Thr Gly Ile                 405 410 415 Leu Ala Ile Ala Leu Ile Leu Leu Phe Gly Ile Val Leu Leu Ser Met             420 425 430 Val Lys Pro Pro His Lys Val Lys         435 440 <210> 269 <211> 840 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (817) <223> RXN00535 <400> 269 aatcgcatgg ggcaccgtgg tcagacaccg gatcgcgctc cgcaccccaa aagatggctc 60 cctaaggagc tcacctttac tcaatgctct gatgacaccg atg tgg tgg gca ggc 115                                             Met Trp Trp Ala Gly                                               1 5 atg agt acc gcg atg ctg gca tat ttc tta caa aca gta gca ctt ggt 163 Met Ser Thr Ala Met Leu Ala Tyr Phe Leu Gln Thr Val Ala Leu Gly                  10 15 20 ttc ggc acc ctc ttg gta gtg caa cca gtg ctt gtc ctg tcg ctg atg 211 Phe Gly Thr Leu Leu Val Val Gln Pro Val Leu Val Leu Ser Leu Met              25 30 35 ttc acg ctg ccg ctc tca gca cga ttc aat ggc tac cga cta cgc cga 259 Phe Thr Leu Pro Leu Ser Ala Arg Phe Asn Gly Tyr Arg Leu Arg Arg          40 45 50 act gaa atc ttc tgg gct acc ctc ctc acc gta gcc gtg ggc atc atg 307 Thr Glu Ile Phe Trp Ala Thr Leu Leu Thr Val Ala Val Gly Ile Met      55 60 65 atc gtt ttg gga cgc ccc ctt ccc gga aac ccc cac ccc cca ctc gat 355 Ile Val Leu Gly Arg Pro Leu Pro Gly Asn Pro His Pro Pro Leu Asp  70 75 80 85 cga tgg att cca gta ctt tta gtc ggc gtt gca gta atg ggt gga atg 403 Arg Trp Ile Pro Val Leu Leu Val Gly Val Ala Val Met Gly Gly Met                  90 95 100 tgg ctg ctt gcg gaa tac gta tta aag aag gac aaa gcc ctc atc ctt 451 Trp Leu Leu Ala Glu Tyr Val Leu Lys Lys Asp Lys Ala Leu Ile Leu             105 110 115 ggt ctt gtg acg ggt gca ttg ttt ggc tac gta gca gtg atg tcc aaa 499 Gly Leu Val Thr Gly Ala Leu Phe Gly Tyr Val Ala Val Met Ser Lys         120 125 130 gcc gcg gtg gat ctt ttt gtc cat caa ggc ata acg gga ctc atc ttg 547 Ala Ala Val Asp Leu Phe Val His Gln Gly Ile Thr Gly Leu Ile Leu     135 140 145 aac tgg gaa ggc tac ggc cta atc ctc acc gca tta ctt gga aca atc 595 Asn Trp Glu Gly Tyr Gly Leu Ile Leu Thr Ala Leu Leu Gly Thr Ile 150 155 160 165 gtg cag cag tat tcc ttt aac gct ggc gaa cta caa aaa tcg cta ccc 643 Val Gln Gln Tyr Ser Phe Asn Ala Gly Glu Leu Gln Lys Ser Leu Pro                 170 175 180 gcc atg acc att gcc gaa cca att gtt gcc ttc agt ttg ggc tac ttg 691 Ala Met Thr Ile Ala Glu Pro Ile Val Ala Phe Ser Leu Gly Tyr Leu             185 190 195 gtt ctg ggc gaa aaa ttc caa gtc gtg gac tgg gaa tgg atc gcc atg 739 Val Leu Gly Glu Lys Phe Gln Val Val Asp Trp Glu Trp Ile Ala Met         200 205 210 ggc atc gca cta ctg gtg atg att gtt tcc acc att gca ctg tct cgt 787 Gly Ile Ala Leu Leu Val Met Ile Val Ser Thr Ile Ala Leu Ser Arg     215 220 225 aca agc aca atg ccg gcc gga tcg aaa agg taaaactcca aagttccccc 837 Thr Ser Thr Met Pro Ala Gly Ser Lys Arg 230 235 cga 840 <210> 270 <211> 239 <212> PRT <213> Corynebacterium glutamicum <400> 270 Met Trp Trp Ala Gly Met Ser Thr Ala Met Leu Ala Tyr Phe Leu Gln   1 5 10 15 Thr Val Ala Leu Gly Phe Gly Thr Leu Leu Val Val Gln Pro Val Leu              20 25 30 Val Leu Ser Leu Met Phe Thr Leu Pro Leu Ser Ala Arg Phe Asn Gly          35 40 45 Tyr Arg Leu Arg Arg Thr Glu Ile Phe Trp Ala Thr Leu Leu Thr Val      50 55 60 Ala Val Gly Ile Met Ile Val Leu Gly Arg Pro Leu Pro Gly Asn Pro  65 70 75 80 His Pro Pro Leu Asp Arg Trp Ile Pro Val Leu Leu Val Gly Val Ala                  85 90 95 Val Met Gly Gly Met Trp Leu Leu Ala Glu Tyr Val Leu Lys Lys Asp             100 105 110 Lys Ala Leu Ile Leu Gly Leu Val Thr Gly Ala Leu Phe Gly Tyr Val         115 120 125 Ala Val Met Ser Lys Ala Ala Val Asp Leu Phe Val His Gln Gly Ile     130 135 140 Thr Gly Leu Ile Leu Asn Trp Glu Gly Tyr Gly Leu Ile Leu Thr Ala 145 150 155 160 Leu Leu Gly Thr Ile Val Gln Gln Tyr Ser Phe Asn Ala Gly Glu Leu                 165 170 175 Gln Lys Ser Leu Pro Ala Met Thr Ile Ala Glu Pro Ile Val Ala Phe             180 185 190 Ser Leu Gly Tyr Leu Val Leu Gly Glu Lys Phe Gln Val Val Asp Trp         195 200 205 Glu Trp Ile Ala Met Gly Ile Ala Leu Leu Val Met Ile Val Ser Thr     210 215 220 Ile Ala Leu Ser Arg Thr Ser Thr Met Pro Ala Gly Ser Lys Arg 225 230 235 <210> 271 <211> 2472 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (2449) <223> RXN00453 <400> 271 tagtggggcg tgaaaaaata gctcatttaa gaggagaagc aaccccgtgg cgaaattgct 60 attcaggttg gggcgatggt cctataatcg caagtggatt gtg att tcg gca tgg 115                                             Val Ile Ser Ala Trp                                               1 5 cta ctt att ttg gcc att gtt ggt ggt ctg gcc ctg acg atg cag aag 163 Leu Leu Ile Leu Ala Ile Val Gly Gly Leu Ala Leu Thr Met Gln Lys                  10 15 20 ggg ttc agt aac tct ttc act att gaa gac acc cct tcg att gat gcc 211 Gly Phe Ser Asn Ser Phe Thr Ile Glu Asp Thr Pro Ser Ile Asp Ala              25 30 35 act gtt tct ctg gtt gaa aat ttc cct gat cag acg aac ccg gtg acg 259 Thr Val Ser Leu Val Glu Asn Phe Pro Asp Gln Thr Asn Pro Val Thr          40 45 50 gcc gcc gga gtt aac gtg gtt ttc caa tcc ccg gaa gga acc acg ctt 307 Ala Ala Gly Val Asn Val Val Phe Gln Ser Pro Glu Gly Thr Thr Leu      55 60 65 gat gat cct cag atg atg act gcg atg gat gca gtc gtt gat tac att 355 Asp Asp Pro Gln Met Met Thr Ala Met Asp Ala Val Val Asp Tyr Ile  70 75 80 85 gag gac aat ttg cct gat ttt ggt ggg gga gag cgc ttc ggc aat cct 403 Glu Asp Asn Leu Pro Asp Phe Gly Gly Gly Glu Arg Phe Gly Asn Pro                  90 95 100 gtt gag gtg tct cct gcg ttg gaa gag atg gtc atc gag cag atg acc 451 Val Glu Val Ser Pro Ala Leu Glu Glu Met Val Ile Glu Gln Met Thr             105 110 115 agc atg ggg ctt cct gag gaa acc gct gca aag gat gct gcc aat ctg 499 Ser Met Gly Leu Pro Glu Glu Thr Ala Ala Lys Asp Ala Ala Asn Leu         120 125 130 gcg gtg ttg agc gaa gac aaa acc att ggc tac acc tct ttc aac att 547 Ala Val Leu Ser Glu Asp Lys Thr Ile Gly Tyr Thr Ser Phe Asn Ile     135 140 145 gat gtt gag gcc gca gaa tat gtg gag caa aaa cac cgc gat gtg atc 595 Asp Val Glu Ala Ala Glu Tyr Val Glu Gln Lys His Arg Asp Val Ile 150 155 160 165 aac gaa gcg atg caa atc ggt gaa gat tta ggt gtc cgg gtg gaa gcc 643 Asn Glu Ala Met Gln Ile Gly Glu Asp Leu Gly Val Arg Val Glu Ala                 170 175 180 ggt gga cct gct ttc ggt gat cca att cag att gaa acc acc agt gag 691 Gly Gly Pro Ala Phe Gly Asp Pro Ile Gln Ile Glu Thr Thr Ser Glu             185 190 195 atc atc ggt att ggc atc gcg ttc atc gtg ttg att ttc acc ttt ggt 739 Ile Ile Gly Ile Gly Ile Ala Phe Ile Val Leu Ile Phe Thr Phe Gly         200 205 210 tct ttg att gct gca ggc ttg cct ttg att acc gcg gtg atc ggc gtg 787 Ser Leu Ile Ala Ala Gly Leu Pro Leu Ile Thr Ala Val Ile Gly Val     215 220 225 ggc att ggt gcg ctg gca att gtg ctg gcc acg gcg ttt act gat ctc 835 Gly Ile Gly Ala Leu Ala Ile Val Leu Ala Thr Ala Phe Thr Asp Leu 230 235 240 245 aac aat gtg act cca gtg ctc gca gtg atg att ggc ctg gcc gtg ggc 883 Asn Asn Val Thr Pro Val Leu Ala Val Met Ile Gly Leu Ala Val Gly                 250 255 260 att gac tac gcg ctg ttt att ttg tct agg tac cgt gcg gag tat aag 931 Ile Asp Tyr Ala Leu Phe Ile Leu Ser Arg Tyr Arg Ala Glu Tyr Lys             265 270 275 cgc atg cca cgt gcc gat gct gcc gga atg gcg gtg ggc aca gct ggt 979 Arg Met Pro Arg Ala Asp Ala Ala Gly Met Ala Val Gly Thr Ala Gly         280 285 290 agt gcg gtg gtg ttt gct ggc gcg acg gtg att atc gcg ctg gta gcc 1027 Ser Ala Val Val Phe Ala Gly Ala Thr Val Ile Ile Ala Leu Val Ala     295 300 305 ctc atc att gcg gat atc gga ttc ctc acg gcc atg ggt att tct gcg 1075 Leu Ile Ile Ala Asp Ile Gly Phe Leu Thr Ala Met Gly Ile Ser Ala 310 315 320 325 gcg ttt acg gtg ttc gtg gct gtg ctc att gcg ttg acg ttt atc ccg 1123 Ala Phe Thr Val Phe Val Ala Val Leu Ile Ala Leu Thr Phe Ile Pro                 330 335 340 gcg ctg ttg ggt gtg ttt ggt ggt cat gcg ttc aag ggc aag atc cct 1171 Ala Leu Leu Gly Val Phe Gly Gly His Ala Phe Lys Gly Lys Ile Pro             345 350 355 gga att ggt gga aac cca acg cca aag cag acg tgg gag caa gcg ctt 1219 Gly Ile Gly Gly Asn Pro Thr Pro Lys Gln Thr Trp Glu Gln Ala Leu         360 365 370 aat cgt cgt tcc aag ggt cgc tca tgg gtc aag ctt gta cag aaa gca 1267 Asn Arg Arg Ser Lys Gly Arg Ser Trp Val Lys Leu Val Gln Lys Ala     375 380 385 ccg ggt ctt gtg gtg gca gtg gtg gtc ttg ggt ctt ggt gcc ttg acc 1315 Pro Gly Leu Val Val Ala Val Val Val Leu Gly Leu Gly Ala Leu Thr 390 395 400 405 att cct gca atg aac ctg cag ttg tca ctg cct tct gac tcc acc tcc 1363 Ile Pro Ala Met Asn Leu Gln Leu Ser Leu Pro Ser Asp Ser Thr Ser                 410 415 420 aat att gat acc act cag cgt cag tcg gct gat ttg atg gca gag ggc 1411 Asn Ile Asp Thr Thr Gln Arg Gln Ser Ala Asp Leu Met Ala Glu Gly             425 430 435 ttt ggc gcg ggc gtt aat gcg ccg ttc ttg gtc atc gtc gat acg cat 1459 Phe Gly Ala Gly Val Asn Ala Pro Phe Leu Val Ile Val Asp Thr His         440 445 450 gag gtc aat gct gat tcc acc gca ttg cag cca ctg att gag gca cag 1507 Glu Val Asn Ala Asp Ser Thr Ala Leu Gln Pro Leu Ile Glu Ala Gln     455 460 465 gag cct gaa gag ggc gag ttc gat cgg gag cag gcg gct cgt ttt gct 1555 Glu Pro Glu Glu Gly Glu Phe Asp Arg Glu Gln Ala Ala Arg Phe Ala 470 475 480 485 acc tat atg tat gtc acc cag acc tac aat tcc aac atc gat gtg aag 1603 Thr Tyr Met Tyr Val Thr Gln Thr Tyr Asn Ser Asn Ile Asp Val Lys                 490 495 500 aat gcg cag atc atc agc gtc aat gat gat ttc act gcg gcg cag att 1651 Asn Ala Gln Ile Ile Ser Val Asn Asp Asp Phe Thr Ala Ala Gln Ile             505 510 515 ctc gtg act cca tac acc gga cct gcg gat aaa gag acc cct gag ttg 1699 Leu Val Thr Pro Tyr Thr Gly Pro Ala Asp Lys Glu Thr Pro Glu Leu         520 525 530 atg cac gtg ctg cgt gcg cag gaa gct cag att gag gat gtt acg gga 1747 Met His Val Leu Arg Ala Gln Glu Ala Gln Ile Glu Asp Val Thr Gly     535 540 545 act gaa ctg ggt acc act ggg ttt acg gcg gtt cag ttg gac att act 1795 Thr Glu Leu Gly Thr Thr Gly Phe Thr Ala Val Gln Leu Asp Ile Thr 550 555 560 565 gag cag ctg gaa gac gca atg ccg gtt tac ctc gct gtg gtt gtt ggt 1843 Glu Gln Leu Glu Asp Ala Met Pro Val Tyr Leu Ala Val Val Val Gly                 570 575 580 ttg gct att ttc ctc ctc att ctg gtg ttc cgt tcc ctg ctt gtt ccg 1891 Leu Ala Ile Phe Leu Leu Ile Leu Val Phe Arg Ser Leu Leu Val Pro             585 590 595 ctg gtt gct ggc ctt ggc ttc ttg ttg tct gtg ggt gcg gcc ttc ggt 1939 Leu Val Ala Gly Leu Gly Phe Leu Leu Ser Val Gly Ala Ala Phe Gly         600 605 610 gcg acg gtg ttg gtc tgg cag gag ggc ttc ggt ggc ttt gtg aac acc 1987 Ala Thr Val Leu Val Trp Gln Glu Gly Phe Gly Gly Phe Val Asn Thr     615 620 625 cct ggt ccg ctg att tcc ttc atg ccg atc ttc ctc atc ggc gtg acc 2035 Pro Gly Pro Leu Ile Ser Phe Met Pro Ile Phe Leu Ile Gly Val Thr 630 635 640 645 ttc ggt ttg gcc atg gac tat cag gtg ttc ctt gtg act cgc atg cgc 2083 Phe Gly Leu Ala Met Asp Tyr Gln Val Phe Leu Val Thr Arg Met Arg                 650 655 660 gag cac tac acc cac cac aat ggc aag gga cag cct ggt tcc aag tac 2131 Glu His Tyr Thr His His Asn Gly Lys Gly Gln Pro Gly Ser Lys Tyr             665 670 675 acc ccg gtt gag cag tca gtg att gaa ggc ttc acg cag ggc tcc cgc 2179 Thr Pro Val Glu Gln Ser Val Ile Glu Gly Phe Thr Gln Gly Ser Arg         680 685 690 gtg gtt aca gca gcg gca ctg atc atg att gcc gtg ttc gtg gcg ttt 2227 Val Val Thr Ala Ala Ala Leu Ile Met Ile Ala Val Phe Val Ala Phe     695 700 705 att gat cag ccg ttg cca ttt att aag atc ttc ggt ttc gcg ttg ggt 2275 Ile Asp Gln Pro Leu Pro Phe Ile Lys Ile Phe Gly Phe Ala Leu Gly 710 715 720 725 gcg ggc gtg ttt ttc gat gct ttc ttc att cgc atg ggt ctg gtc ccc 2323 Ala Gly Val Phe Phe Asp Ala Phe Phe Ile Arg Met Gly Leu Val Pro                 730 735 740 gcg tcg atg ttc ctg atg ggc aag gcc acg tgg tgg atg cct aag tgg 2371 Ala Ser Met Phe Leu Met Gly Lys Ala Thr Trp Trp Met Pro Lys Trp             745 750 755 ctg gat cga att ctg cca agt ttg gac att gaa ggc acc gca ctg gag 2419 Leu Asp Arg Ile Leu Pro Ser Leu Asp Ile Glu Gly Thr Ala Leu Glu         760 765 770 aag gaa tgg gag gag aag cag gct gca cgt tagacttggc acctatgtca 2469 Lys Glu Trp Glu Glu Lys Gln Ala Ala Arg     775 780 gat 2472 <210> 272 <211> 783 <212> PRT <213> Corynebacterium glutamicum <400> 272 Val Ile Ser Ala Trp Leu Leu Ile Leu Ala Ile Val Gly Gly Leu Ala   1 5 10 15 Leu Thr Met Gln Lys Gly Phe Ser Asn Ser Phe Thr Ile Glu Asp Thr              20 25 30 Pro Ser Ile Asp Ala Thr Val Ser Leu Val Glu Asn Phe Pro Asp Gln          35 40 45 Thr Asn Pro Val Thr Ala Ala Gly Val Asn Val Val Phe Gln Ser Pro      50 55 60 Glu Gly Thr Thr Leu Asp Asp Pro Gln Met Met Thr Ala Met Asp Ala  65 70 75 80 Val Val Asp Tyr Ile Glu Asp Asn Leu Pro Asp Phe Gly Gly Gly Glu                  85 90 95 Arg Phe Gly Asn Pro Val Glu Val Ser Pro Ala Leu Glu Glu Met Val             100 105 110 Ile Glu Gln Met Thr Ser Met Gly Leu Pro Glu Glu Thr Ala Ala Lys         115 120 125 Asp Ala Ala Asn Leu Ala Val Leu Ser Glu Asp Lys Thr Ile Gly Tyr     130 135 140 Thr Ser Phe Asn Ile Asp Val Glu Ala Ala Glu Tyr Val Glu Gln Lys 145 150 155 160 His Arg Asp Val Ile Asn Glu Ala Met Gln Ile Gly Glu Asp Leu Gly                 165 170 175 Val Arg Val Glu Ala Gly Gly Pro Ala Phe Gly Asp Pro Ile Gln Ile             180 185 190 Glu Thr Thr Ser Glu Ile Ile Gly Ile Gly Ile Ala Phe Ile Val Leu         195 200 205 Ile Phe Thr Phe Gly Ser Leu Ile Ala Ala Gly Leu Pro Leu Ile Thr     210 215 220 Ala Val Ile Gly Val Gly Ile Gly Ala Leu Ala Ile Val Leu Ala Thr 225 230 235 240 Ala Phe Thr Asp Leu Asn Asn Val Thr Pro Val Leu Ala Val Met Ile                 245 250 255 Gly Leu Ala Val Gly Ile Asp Tyr Ala Leu Phe Ile Leu Ser Arg Tyr             260 265 270 Arg Ala Glu Tyr Lys Arg Met Pro Arg Ala Asp Ala Ala Gly Met Ala         275 280 285 Val Gly Thr Ala Gly Ser Ala Val Val Phe Ala Gly Ala Thr Val Ile     290 295 300 Ile Ala Leu Val Ala Leu Ile Ile Ala Asp Ile Gly Phe Leu Thr Ala 305 310 315 320 Met Gly Ile Ser Ala Ala Phe Thr Val Phe Val Ala Val Leu Ile Ala                 325 330 335 Leu Thr Phe Ile Pro Ala Leu Leu Gly Val Phe Gly Gly His Ala Phe             340 345 350 Lys Gly Lys Ile Pro Gly Ile Gly Gly Asn Pro Thr Pro Lys Gln Thr         355 360 365 Trp Glu Gln Ala Leu Asn Arg Arg Ser Lys Gly Arg Ser Trp Val Lys     370 375 380 Leu Val Gln Lys Ala Pro Gly Leu Val Val Ala Val Val Leu Gly 385 390 395 400 Leu Gly Ala Leu Thr Ile Pro Ala Met Asn Leu Gln Leu Ser Leu Pro                 405 410 415 Ser Asp Ser Thr Ser Asn Ile Asp Thr Thr Gln Arg Gln Ser Ala Asp             420 425 430 Leu Met Ala Glu Gly Phe Gly Ala Gly Val Asn Ala Pro Phe Leu Val         435 440 445 Ile Val Asp Thr His Glu Val Asn Ala Asp Ser Thr Ala Leu Gln Pro     450 455 460 Leu Ile Glu Ala Gln Glu Pro Glu Glu Gly Glu Phe Asp Arg Glu Gln 465 470 475 480 Ala Ala Arg Phe Ala Thr Tyr Met Tyr Val Thr Gln Thr Tyr Asn Ser                 485 490 495 Asn Ile Asp Val Lys Asn Ala Gln Ile Ile Ser Val Asn Asp Asp Phe             500 505 510 Thr Ala Ala Gln Ile Leu Val Thr Pro Tyr Thr Gly Pro Ala Asp Lys         515 520 525 Glu Thr Pro Glu Leu Met His Val Leu Arg Ala Gln Glu Ala Gln Ile     530 535 540 Glu Asp Val Thr Gly Thr Glu Leu Gly Thr Thr Gly Phe Thr Ala Val 545 550 555 560 Gln Leu Asp Ile Thr Glu Gln Leu Glu Asp Ala Met Pro Val Tyr Leu                 565 570 575 Ala Val Val Val Gly Leu Ala Ile Phe Leu Leu Ile Leu Val Phe Arg             580 585 590 Ser Leu Leu Val Pro Leu Val Ala Gly Leu Gly Phe Leu Leu Ser Val         595 600 605 Gly Ala Ala Phe Gly Ala Thr Val Leu Val Trp Gln Glu Gly Phe Gly     610 615 620 Gly Phe Val Asn Thr Pro Gly Pro Leu Ile Ser Phe Met Pro Ile Phe 625 630 635 640 Leu Ile Gly Val Thr Phe Gly Leu Ala Met Asp Tyr Gln Val Phe Leu                 645 650 655 Val Thr Arg Met Arg Glu His Tyr Thr His His Asn Gly Lys Gly Gln             660 665 670 Pro Gly Ser Lys Tyr Thr Pro Val Glu Gln Ser Val Ile Glu Gly Phe         675 680 685 Thr Gln Gly Ser Arg Val Val Thr Ala Ala Ala Leu Ile Met Ile Ala     690 695 700 Val Phe Val Ala Phe Ile Asp Gln Pro Leu Pro Phe Ile Lys Ile Phe 705 710 715 720 Gly Phe Ala Leu Gly Ala Gly Val Phe Phe Asp Ala Phe Phe Ile Arg                 725 730 735 Met Gly Leu Val Pro Ala Ser Met Phe Leu Met Gly Lys Ala Thr Trp             740 745 750 Trp Met Pro Lys Trp Leu Asp Arg Ile Leu Pro Ser Leu Asp Ile Glu         755 760 765 Gly Thr Ala Leu Glu Lys Glu Trp Glu Glu Lys Gln Ala Ala Arg     770 775 780 <210> 273 <211> 597 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (574) <223> RXN00932 <400> 273 cccaattaat ttatgcactt cggtgaggtt actcacaaag agtagcgtgc aaagcccagc 60 aataaggtga tgtttcaacg attaggttac ggtaggggcc atg acg cca cag aaa 115                                             Met Thr Pro Gln Lys                                               1 5 ctt cac cgt ttt gca gcc ctt tta gaa atg ggt acc tgg acc ctg ctg 163 Leu His Arg Phe Ala Ala Leu Leu Glu Met Gly Thr Trp Thr Leu Leu                  10 15 20 atc atc ggc atg atc tta aaa tac agt gga gtg aca gac gcc gta acc 211 Ile Ile Gly Met Ile Leu Lys Tyr Ser Gly Val Thr Asp Ala Val Thr              25 30 35 cct att gcc ggc ggt atc cac ggc ttt ggc ttc ctc tgt ttt gca gcc 259 Pro Ile Ala Gly Gly Ile His Gly Phe Gly Phe Leu Cys Phe Ala Ala          40 45 50 atc acc atc acc gtg tgg atc aat aat aag tgg aca ttc ccg cag ggt 307 Ile Thr Ile Thr Val Trp Ile Asn Asn Lys Trp Thr Phe Pro Gln Gly      55 60 65 atc gca ggt ttg atc gtc tct gtt atc ccg tgg gct gca ttg cca ttt 355 Ile Ala Gly Leu Ile Val Ser Val Ile Pro Trp Ala Ala Leu Pro Phe  70 75 80 85 gca ttg tgg gca gac aag aag ggc ctc gtt gcc ggc gga tgg cgc ttt 403 Ala Leu Trp Ala Asp Lys Lys Gly Leu Val Ala Gly Gly Trp Arg Phe                  90 95 100 tca gat ccg tcc gaa aag cca cac act ttc ttt gac aag atc ttg gct 451 Ser Asp Pro Ser Glu Lys Pro His Thr Phe Phe Asp Lys Ile Leu Ala             105 110 115 caa ttg gtc agg cac cca atc cga tcc att tta att ctg ctg gtg att 499 Gln Leu Val Arg His Pro Ile Arg Ser Ile Leu Ile Leu Leu Val Ile         120 125 130 atc gcc gtc gtc ttc tct atc ttg ctg gcg atg gga cca cct tat gat 547 Ile Ala Val Val Phe Ser Ile Leu Leu Ala Met Gly Pro Pro Tyr Asp     135 140 145 cca gat gcc atc gca aac act gtg gat taaacaacag cctccttcac 594 Pro Asp Ala Ile Ala Asn Thr Val Asp 150 155 atg 597 <210> 274 <211> 158 <212> PRT <213> Corynebacterium glutamicum <400> 274 Met Thr Pro Gln Lys Leu His Arg Phe Ala Ala Leu Leu Glu Met Gly   1 5 10 15 Thr Trp Thr Leu Leu Ile Ile Gly Met Ile Leu Lys Tyr Ser Gly Val              20 25 30 Thr Asp Ala Val Thr Pro Ile Ala Gly Gly Ile His Gly Phe Gly Phe          35 40 45 Leu Cys Phe Ala Ala Ile Thr Ile Thr Val Trp Ile Asn Asn Lys Trp      50 55 60 Thr Phe Pro Gln Gly Ile Ala Gly Leu Ile Val Ser Val Ile Pro Trp  65 70 75 80 Ala Ala Leu Pro Phe Ala Leu Trp Ala Asp Lys Lys Gly Leu Val Ala                  85 90 95 Gly Gly Trp Arg Phe Ser Asp Pro Ser Glu Lys Pro His Thr Phe Phe             100 105 110 Asp Lys Ile Leu Ala Gln Leu Val Arg His Pro Ile Arg Ser Ile Leu         115 120 125 Ile Leu Leu Val Ile Ile Ala Val Val Phe Ser Ile Leu Leu Ala Met     130 135 140 Gly Pro Pro Tyr Asp Pro Asp Ala Ile Ala Asn Thr Val Asp 145 150 155 <210> 275 <211> 534 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (65) .. (511) <223> RXN03022 <400> 275 acgcctgtgt catccttttc attagagtgg agaaaagccc atacagaaag ttggcgcccg 60 agca gtg atc atc acc gct ggc atc ttg gta gcg acc gcg acc gcc ctc 109      Val Ile Ile Thr Ala Gly Ile Leu Val Ala Thr Ala Thr Ala Leu       1 5 10 15 cta atg atc acc gcg gtc agc gag tca acg tac atc gtc atc tcc ctc 157 Leu Met Ile Thr Ala Val Ser Glu Ser Thr Tyr Ile Val Ile Ser Leu                  20 25 30 gcc ggc ttc tcc ctt tat ggc ctt ggc ctc gga ctc ttc gcc acc cca 205 Ala Gly Phe Ser Leu Tyr Gly Leu Gly Leu Gly Leu Phe Ala Thr Pro              35 40 45 gtc acc gat act gcg ctt gga aca ctt ccc aaa gac cgt acc ggc gct 253 Val Thr Asp Thr Ala Leu Gly Thr Leu Pro Lys Asp Arg Thr Gly Ala          50 55 60 ggt gca ggt gta ttc aag atg tcc tct tcc ctc ggc gca gca ctc ggc 301 Gly Ala Gly Val Phe Lys Met Ser Ser Ser Leu Gly Ala Ala Leu Gly      65 70 75 atc gca atc tcc act tca gtg ttc ctc gca ctt cgc gac ggc acc tcc 349 Ile Ala Ile Ser Thr Ser Val Phe Leu Ala Leu Arg Asp Gly Thr Ser  80 85 90 95 atc aac tcc gac gtc gca ctc gcc gga aca gtt tca ctt ggc atc aac 397 Ile Asn Ser Asp Val Ala Leu Ala Gly Thr Val Ser Leu Gly Ile Asn                 100 105 110 gtt gta ttc gca gca aca gcc acc atc acc gca gca gtc ctt att cca 445 Val Val Phe Ala Ala Thr Ala Thr Ile Thr Ala Ala Val Leu Ile Pro             115 120 125 aaa gcc gct ggc aaa gtc tca caa acc agc atc acc ctt cct gag cca 493 Lys Ala Ala Gly Lys Val Ser Gln Thr Ser Ile Thr Leu Pro Glu Pro         130 135 140 gct atc gct gta aaa atc taaaacttca ccaggacaga taa 534 Ala Ile Ala Val Lys Ile     145 <210> 276 <211> 149 <212> PRT <213> Corynebacterium glutamicum <400> 276 Val Ile Ile Thr Ala Gly Ile Leu Val Ala Thr Ala Thr Ala Leu Leu   1 5 10 15 Met Ile Thr Ala Val Ser Glu Ser Thr Tyr Ile Val Ile Ser Leu Ala              20 25 30 Gly Phe Ser Leu Tyr Gly Leu Gly Leu Gly Leu Phe Ala Thr Pro Val          35 40 45 Thr Asp Thr Ala Leu Gly Thr Leu Pro Lys Asp Arg Thr Gly Ala Gly      50 55 60 Ala Gly Val Phe Lys Met Ser Ser Ser Leu Gly Ala Ala Leu Gly Ile  65 70 75 80 Ala Ile Ser Thr Ser Val Phe Leu Ala Leu Arg Asp Gly Thr Ser Ile                  85 90 95 Asn Ser Asp Val Ala Leu Ala Gly Thr Val Ser Leu Gly Ile Asn Val             100 105 110 Val Phe Ala Ala Thr Ala Thr Ile Thr Ala Ala Val Leu Ile Pro Lys         115 120 125 Ala Ala Gly Lys Val Ser Gln Thr Ser Ile Thr Leu Pro Glu Pro Ala     130 135 140 Ile Ala Val Lys Ile 145 <210> 277 <211> 586 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (586) <223> RXN03151 <400> 277 ccgaacttgg agctttgctg ttggaggcag ccaaatagtc ccaatgtaaa cgcactgggt 60 agtatttgtt taaccatcca cctcaaggag taaaacgcac gtg ctt tcc cac atc 115                                             Val Leu Ser His Ile                                               1 5 att gat gtc ctc gcc gac ccg atc gat ggc acc cca ctt gta ggc gcc 163 Ile Asp Val Leu Ala Asp Pro Ile Asp Gly Thr Pro Leu Val Gly Ala                  10 15 20 gaa gat ttc tca cgg ttg gtg tct gaa tct ggg cat tcc tac gat gtt 211 Glu Asp Phe Ser Arg Leu Val Ser Glu Ser Gly His Ser Tyr Asp Val              25 30 35 gct cgt caa ggg tat gtc acc ctg gct ggt ggc gca ggt ctg cgc tat 259 Ala Arg Gln Gly Tyr Val Thr Leu Ala Gly Gly Ala Gly Leu Arg Tyr          40 45 50 tca ggc gat gat gca cag atg atc gcg gat cgg gaa acc ttc ctt tct 307 Ser Gly Asp Asp Ala Gln Met Ile Ala Asp Arg Glu Thr Phe Leu Ser      55 60 65 ggc ggt cac ttc gcg ccc ttc gtg gaa gct gtc acc gag cat gtt caa 355 Gly Gly His Phe Ala Pro Phe Val Glu Ala Val Thr Glu His Val Gln  70 75 80 85 gat gtc gtt gac cag gca ggc ctt agc gat gac gca cag cca gtg gtc 403 Asp Val Val Asp Gln Ala Gly Leu Ser Asp Asp Ala Gln Pro Val Val                  90 95 100 tgc gaa atc ggc gcg gga acc ggc tac tac ttg tcc cat acc ctt gat 451 Cys Glu Ile Gly Ala Gly Thr Gly Tyr Tyr Leu Ser His Thr Leu Asp             105 110 115 tct gtt gca gga tct cgc gga att ggc att gac gtt tcc gtg cac gcc 499 Ser Val Ala Gly Ser Arg Gly Ile Gly Ile Asp Val Ser Val His Ala         120 125 130 gca aag cgt ttg gca aag tgt cac cct cgc gtc ggc gca gtc atc gcg 547 Ala Lys Arg Leu Ala Lys Cys His Pro Arg Val Gly Ala Val Ile Ala     135 140 145 aac gca tgg gca cgc ctg ccg att gca gat aac tcc tcg 586 Asn Ala Trp Ala Arg Leu Pro Ile Ala Asp Asn Ser Ser 150 155 160 <210> 278 <211> 162 <212> PRT <213> Corynebacterium glutamicum <400> 278 Val Leu Ser His Ile Ile Asp Val Leu Ala Asp Pro Ile Asp Gly Thr   1 5 10 15 Pro Leu Val Gly Ala Glu Asp Phe Ser Arg Leu Val Ser Glu Ser Gly              20 25 30 His Ser Tyr Asp Val Ala Arg Gln Gly Tyr Val Thr Leu Ala Gly Gly          35 40 45 Ala Gly Leu Arg Tyr Ser Gly Asp Asp Ala Gln Met Ile Ala Asp Arg      50 55 60 Glu Thr Phe Leu Ser Gly Gly His Phe Ala Pro Phe Val Glu Ala Val  65 70 75 80 Thr Glu His Val Gln Asp Val Val Asp Gln Ala Gly Leu Ser Asp Asp                  85 90 95 Ala Gln Pro Val Val Cys Glu Ile Gly Ala Gly Thr Gly Tyr Tyr Leu             100 105 110 Ser His Thr Leu Asp Ser Val Ala Gly Ser Arg Gly Ile Gly Ile Asp         115 120 125 Val Ser Val His Ala Ala Lys Arg Leu Ala Lys Cys His Pro Arg Val     130 135 140 Gly Ala Val Ile Ala Asn Ala Trp Ala Arg Leu Pro Ile Ala Asp Asn 145 150 155 160 Ser Ser          <210> 279 <211> 543 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (1) .. (543) <223> RXN02832 <400> 279 cgc ggg cca gtg atg gat tat aca aat caa tca tta gta gca ttt ttc 48 Arg Gly Pro Val Met Asp Tyr Thr Asn Gln Ser Leu Val Ala Phe Phe   1 5 10 15 ttt aaa gca tta acg tca tat tta aag aaa cac aat tgt tta tat gtc 96 Phe Lys Ala Leu Thr Ser Tyr Leu Lys Lys His Asn Cys Leu Tyr Val              20 25 30 ctt gta gat cca tat tta att gaa aat tta cgc aat gca gac ggt gaa 144 Leu Val Asp Pro Tyr Leu Ile Glu Asn Leu Arg Asn Ala Asp Gly Glu          35 40 45 att gtt aaa tct tat gat aac cga gca ttt gtt aga aca atg gat aaa 192 Ile Val Lys Ser Tyr Asp Asn Arg Ala Phe Val Arg Thr Met Asp Lys      50 55 60 tta ggt tat aaa cac caa ggt ttc cct gta ggt tat gat tca atg agc 240 Leu Gly Tyr Lys His Gln Gly Phe Pro Val Gly Tyr Asp Ser Met Ser  65 70 75 80 caa atc cgt tgg ctg tca gtg tta gat tta aaa gat aag act gaa gac 288 Gln Ile Arg Trp Leu Ser Val Leu Asp Leu Lys Asp Lys Thr Glu Asp                  85 90 95 caa ctt tta aaa gaa atg gat tat caa acg aga cgt aat att aaa aaa 336 Gln Leu Leu Lys Glu Met Asp Tyr Gln Thr Arg Arg Asn Ile Lys Lys             100 105 110 aca tat gat att ggt gtc aaa act aaa acg tta acg att gat gaa acg 384 Thr Tyr Asp Ile Gly Val Lys Thr Lys Thr Leu Thr Ile Asp Glu Thr         115 120 125 caa act ttt ttc gac tta ttc cat atg gct gag gaa aag cac ggt ttc 432 Gln Thr Phe Phe Asp Leu Phe His Met Ala Glu Glu Lys His Gly Phe     130 135 140 aaa ttc cgt gag tta cca tac ttt gaa gaa atg caa aag tta tac gat 480 Lys Phe Arg Glu Leu Pro Tyr Phe Glu Glu Met Gln Lys Leu Tyr Asp 145 150 155 160 gac cac gcc atg tta aag ttg gcg tat att gat tta aac gag tat tta 528 Asp His Ala Met Leu Lys Leu Ala Tyr Ile Asp Leu Asn Glu Tyr Leu                 165 170 175 aaa acg ttg caa tta 543 Lys Thr Leu Gln Leu             180 <210> 280 <211> 181 <212> PRT <213> Corynebacterium glutamicum <400> 280 Arg Gly Pro Val Met Asp Tyr Thr Asn Gln Ser Leu Val Ala Phe Phe   1 5 10 15 Phe Lys Ala Leu Thr Ser Tyr Leu Lys Lys His Asn Cys Leu Tyr Val              20 25 30 Leu Val Asp Pro Tyr Leu Ile Glu Asn Leu Arg Asn Ala Asp Gly Glu          35 40 45 Ile Val Lys Ser Tyr Asp Asn Arg Ala Phe Val Arg Thr Met Asp Lys      50 55 60 Leu Gly Tyr Lys His Gln Gly Phe Pro Val Gly Tyr Asp Ser Met Ser  65 70 75 80 Gln Ile Arg Trp Leu Ser Val Leu Asp Leu Lys Asp Lys Thr Glu Asp                  85 90 95 Gln Leu Leu Lys Glu Met Asp Tyr Gln Thr Arg Arg Asn Ile Lys Lys             100 105 110 Thr Tyr Asp Ile Gly Val Lys Thr Lys Thr Leu Thr Ile Asp Glu Thr         115 120 125 Gln Thr Phe Phe Asp Leu Phe His Met Ala Glu Glu Lys His Gly Phe     130 135 140 Lys Phe Arg Glu Leu Pro Tyr Phe Glu Glu Met Gln Lys Leu Tyr Asp 145 150 155 160 Asp His Ala Met Leu Lys Leu Ala Tyr Ile Asp Leu Asn Glu Tyr Leu                 165 170 175 Lys Thr Leu Gln Leu             180 <210> 281 <211> 1539 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1516) <223> RXN00165 <400> 281 aaacctcccc gggcccggcg cgcgaccgtc caagatgccg gcgttggatg ccaaattatg 60 gactctcaaa gtggcgttgt cgcagcggcc gtggagcttt gtg gcg tct gct ggc 115                                             Val Ala Ser Ala Gly                                               1 5 atg gcg gcg tct ttt atc tgc aat ggg tta acg cct gtg att gtg ggt 163 Met Ala Ala Ser Phe Ile Cys Asn Gly Leu Thr Pro Val Ile Val Gly                  10 15 20 aag gcg gtg gat gag gct att ggc acg agc gat ctg cag cga ttg tgg 211 Lys Ala Val Asp Glu Ala Ile Gly Thr Ser Asp Leu Gln Arg Leu Trp              25 30 35 ttc tgg att gcc atg ttg gcg gtt ctt ttc tta acg gcg atg acg gtg 259 Phe Trp Ile Ala Met Leu Ala Val Leu Phe Leu Thr Ala Met Thr Val          40 45 50 aac tgg att gct cgg tac atg ttg gtg cgg agc cag cag ttg gtc agc 307 Asn Trp Ile Ala Arg Tyr Met Leu Val Arg Ser Gln Gln Leu Val Ser      55 60 65 cat gat ttg cgc atg ttg gtg act gat cgg att caa gat ccg cgt ggt 355 His Asp Leu Arg Met Leu Val Thr Asp Arg Ile Gln Asp Pro Arg Gly  70 75 80 85 ttt gct gga aaa gag cgc act gcg ggt gga ttg ttg tcg att gcg tca 403 Phe Ala Gly Lys Glu Arg Thr Ala Gly Gly Leu Leu Ser Ile Ala Ser                  90 95 100 tcg gat acg cag cgg gtg ggc gat atc gtc atg atg acg gtg ttc ccg 451 Ser Asp Thr Gln Arg Val Gly Asp Ile Val Met Met Thr Val Phe Pro             105 110 115 gtg gcg gaa ttg gcg tcg att att tat ggc gcc gtg gtg atg tac agc 499 Val Ala Glu Leu Ala Ser Ile Ile Tyr Gly Ala Val Val Met Tyr Ser         120 125 130 att aat ccg tgg ttg agt gtg gct gtg ctg att ggt gga ccg ctg ctg 547 Ile Asn Pro Trp Leu Ser Val Ala Val Leu Ile Gly Gly Pro Leu Leu     135 140 145 gtt gtg gtg gct att cag gtc tca aag ccg ttg cag aag cgt tcg ggt 595 Val Val Val Ala Ile Gln Val Ser Lys Pro Leu Gln Lys Arg Ser Gly 150 155 160 165 gct cgt cag cag gcg gtg gca cag gct gcg gct act gca act gat gtg 643 Ala Arg Gln Gln Ala Val Ala Gln Ala Ala Ala Thr Ala Thr Asp Val                 170 175 180 gtg cag ggc ttg aga att ttg aag ggt ttg ggc gcg att gtc acg gtg 691 Val Gln Gly Leu Arg Ile Leu Lys Gly Leu Gly Ala Ile Val Thr Val             185 190 195 cgc cgt cgg tac gag gcg att tct ggt gag gct tat cgg aag acg gtt 739 Arg Arg Arg Tyr Glu Ala Ile Ser Gly Glu Ala Tyr Arg Lys Thr Val         200 205 210 cat gcg gat gct gcg gaa gct cgc ttg aat ggt gtc acc gat gcg gcg 787 His Ala Asp Ala Ala Glu Ala Arg Leu Asn Gly Val Thr Asp Ala Ala     215 220 225 ggc gcc atc ttt gtg tcc gcg ttg ggt att gga gca gga ttt ttg gcg 835 Gly Ala Ile Phe Val Ser Ala Leu Gly Ile Gly Ala Gly Phe Leu Ala 230 235 240 245 ctg caa ggt cag atg agt att ggt gat ttg atc acg gtt gtg gga ctc 883 Leu Gln Gly Gln Met Ser Ile Gly Asp Leu Ile Thr Val Val Gly Leu                 250 255 260 aca cag ttt ttg atc atg ccg atg acc atg ctt ggt cga aat gtg gca 931 Thr Gln Phe Leu Ile Met Pro Met Thr Met Leu Gly Arg Asn Val Ala             265 270 275 tcg cgc tgg gca tcg gcg gag gcg tcg gca aag cgt att agg gga gtg 979 Ser Arg Trp Ala Ser Ala Glu Ala Ser Ala Lys Arg Ile Arg Gly Val         280 285 290 ctc ggt gct gat ttt gag aga gtg tct gcg cat gat gcg gac aag gct 1027 Leu Gly Ala Asp Phe Glu Arg Val Ser Ala His Asp Ala Asp Lys Ala     295 300 305 gag gag att atc caa caa ctt gcc aaa ggt ttg acg gtt att cga ggc 1075 Glu Glu Ile Ile Gln Gln Leu Ala Lys Gly Leu Thr Val Ile Arg Gly 310 315 320 325 act gat gag cag ctc gtt gag gta tta gag cag ttg cca cgt act cgg 1123 Thr Asp Glu Gln Leu Val Glu Val Leu Glu Gln Leu Pro Arg Thr Arg                 330 335 340 gtg att gtg gct cct cat gcg gcg gat ctt ttt gat caa agt gtc agg 1171 Val Ile Val Ala Pro His Ala Ala Asp Leu Phe Asp Gln Ser Val Arg             345 350 355 gac aat gtg cat ccc gtg gca gag gtc gcg gag aaa gcc att gaa gtt 1219 Asp Asn Val His Pro Val Ala Glu Val Ala Glu Lys Ala Ile Glu Val         360 365 370 gcc tcc tgt gac gat att cca ggt ggt agt tcc aag att gtg ggc gag 1267 Ala Ser Cys Asp Asp Ile Pro Gly Gly Ser Ser Lys Ile Val Gly Glu     375 380 385 ggt gga cgg ttg ctc tcg ggt ggt cag cgt cag cgc gtt gca ctg gct 1315 Gly Gly Arg Leu Leu Ser Gly Gly Gln Arg Gln Arg Val Ala Leu Ala 390 395 400 405 cgg gcg att gct ttt gat cca gag gtg ttg gtg ctt caa gat ccc aca 1363 Arg Ala Ile Ala Phe Asp Pro Glu Val Leu Val Leu Gln Asp Pro Thr                 410 415 420 acg gca gtg gat tct gtg acg gag caa aac att gct cag caa gtg gca 1411 Thr Ala Val Asp Ser Val Thr Glu Gln Asn Ile Ala Gln Gln Val Ala             425 430 435 gca cac cgt gca gga aaa gtg acc att gtg ttt agt gag gca ccc gcg 1459 Ala His Arg Ala Gly Lys Val Thr Ile Val Phe Ser Glu Ala Pro Ala         440 445 450 tgg agt gcg gtg gct gat caa cac gtt gag gca gct gct ttg cgg gag 1507 Trp Ser Ala Val Ala Asp Gln His Val Glu Ala Ala Ala Leu Arg Glu     455 460 465 gtt atg aaa tgagtgggga gacgtcgaaa agc 1539 Val met lys 470 <210> 282 <211> 472 <212> PRT <213> Corynebacterium glutamicum <400> 282 Val Ala Ser Ala Gly Met Ala Ala Ser Phe Ile Cys Asn Gly Leu Thr   1 5 10 15 Pro Val Ile Val Gly Lys Ala Val Asp Glu Ala Ile Gly Thr Ser Asp              20 25 30 Leu Gln Arg Leu Trp Phe Trp Ile Ala Met Leu Ala Val Leu Phe Leu          35 40 45 Thr Ala Met Thr Val Asn Trp Ile Ala Arg Tyr Met Leu Val Arg Ser      50 55 60 Gln Gln Leu Val Ser His Asp Leu Arg Met Leu Val Thr Asp Arg Ile  65 70 75 80 Gln Asp Pro Arg Gly Phe Ala Gly Lys Glu Arg Thr Ala Gly Gly Leu                  85 90 95 Leu Ser Ile Ala Ser Ser Asp Thr Gln Arg Val Gly Asp Ile Val Met             100 105 110 Met Thr Val Phe Pro Val Ala Glu Leu Ala Ser Ile Ile Tyr Gly Ala         115 120 125 Val Val Met Tyr Ser Ile Asn Pro Trp Leu Ser Val Ala Val Leu Ile     130 135 140 Gly Gly Pro Leu Leu Val Val Val Ala Ile Gln Val Ser Lys Pro Leu 145 150 155 160 Gln Lys Arg Ser Gly Ala Arg Gln Gln Ala Val Ala Gln Ala Ala Ala                 165 170 175 Thr Ala Thr Asp Val Val Gln Gly Leu Arg Ile Leu Lys Gly Leu Gly             180 185 190 Ala Ile Val Thr Val Arg Arg Arg Tyr Glu Ala Ile Ser Gly Glu Ala         195 200 205 Tyr Arg Lys Thr Val His Ala Asp Ala Ala Glu Ala Arg Leu Asn Gly     210 215 220 Val Thr Asp Ala Ala Gly Ala Ile Phe Val Ser Ala Leu Gly Ile Gly 225 230 235 240 Ala Gly Phe Leu Ala Leu Gln Gly Gln Met Ser Ile Gly Asp Leu Ile                 245 250 255 Thr Val Val Gly Leu Thr Gln Phe Leu Ile Met Pro Met Thr Met Leu             260 265 270 Gly Arg Asn Val Ala Ser Arg Trp Ala Ser Ala Glu Ala Ser Ala Lys         275 280 285 Arg Ile Arg Gly Val Leu Gly Ala Asp Phe Glu Arg Val Ser Ala His     290 295 300 Asp Ala Asp Lys Ala Glu Glu Ile Ile Gln Gln Leu Ala Lys Gly Leu 305 310 315 320 Thr Val Ile Arg Gly Thr Asp Glu Gln Leu Val Glu Val Leu Glu Gln                 325 330 335 Leu Pro Arg Thr Arg Val Ile Val Ala Pro His Ala Ala Asp Leu Phe             340 345 350 Asp Gln Ser Val Arg Asp Asn Val His Pro Val Ala Glu Val Ala Glu         355 360 365 Lys Ala Ile Glu Val Ala Ser Cys Asp Asp Ile Pro Gly Gly Ser Ser     370 375 380 Lys Ile Val Gly Glu Gly Gly Arg Leu Leu Ser Gly Gly Gln Arg Gln 385 390 395 400 Arg Val Ala Leu Ala Arg Ala Ile Ala Phe Asp Pro Glu Val Leu Val                 405 410 415 Leu Gln Asp Pro Thr Thr Ala Val Asp Ser Val Thr Glu Gln Asn Ile             420 425 430 Ala Gln Gln Val Ala Ala His Arg Ala Gly Lys Val Thr Ile Val Phe         435 440 445 Ser Glu Ala Pro Ala Trp Ser Ala Val Ala Asp Gln His Val Glu Ala     450 455 460 Ala Ala Leu Arg Glu Val Met Lys 465 470 <210> 283 <211> 1470 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1447) <223> RXN01190 <400> 283 cagggttttg atgagaacaa cacacaccgc ttcaagcatt ctgcgaagaa tgatcaggcg 60 gcagcggggc aaggttgcgt ttggcgcatt ctttttgggg atg tgg cag ctg tcg 115                                             Met Trp Gln Leu Ser                                               1 5 gaa gca ttg gtg ccg att gcg att ggt ttg atc gtt gat cat gcg gtt 163 Glu Ala Leu Val Pro Ile Ala Ile Gly Leu Ile Val Asp His Ala Val                  10 15 20 ctc aca aaa gat ctc cgc cga tta gtg gtc ggg ctt gtc gct ttt gtt 211 Leu Thr Lys Asp Leu Arg Arg Leu Val Val Gly Leu Val Ala Phe Val              25 30 35 gtg ctg ttt gtg gtg ttg agt ttt tct aat cgt ttc ggt tcg cgc gcg 259 Val Leu Phe Val Val Leu Ser Phe Ser Asn Arg Phe Gly Ser Arg Ala          40 45 50 ttg aat agg gcc gtg aac ttt gaa tcc cat gcg ctc cgc gta gag gta 307 Leu Asn Arg Ala Val Asn Phe Glu Ser His Ala Leu Arg Val Glu Val      55 60 65 gcc gat cat gcg ttg aag aat ctg gat ccg cgc aat ttg gtg cct ggc 355 Ala Asp His Ala Leu Lys Asn Leu Asp Pro Arg Asn Leu Val Pro Gly  70 75 80 85 gag gtg atg tcg cgg tcc acc gca gat gcg gat tct tcg acg cgt att 403 Glu Val Met Ser Arg Ser Thr Ala Asp Ala Asp Ser Ser Thr Arg Ile                  90 95 100 ttc ggg cag atc gga acc ggt gtt tcg gct gcg acg gga ttt ctt ggt 451 Phe Gly Gln Ile Gly Thr Gly Val Ser Ala Ala Thr Gly Phe Leu Gly             105 110 115 gca gcg acc tac ctg ttg atc agt gac tgg ctg gtc ggg ttg ttg gtg 499 Ala Ala Thr Tyr Leu Leu Ile Ser Asp Trp Leu Val Gly Leu Leu Val         120 125 130 ctt gtg ctg gta ccg atc att tcg gga gtg gtt gca ctg gct agc aag 547 Leu Val Leu Val Pro Ile Ile Ser Gly Val Val Ala Leu Ala Ser Lys     135 140 145 ggc att tct aaa agg agt gtc acc cag cag gag aag ttg gcg gag tct 595 Gly Ile Ser Lys Arg Ser Val Thr Gln Gln Glu Lys Leu Ala Glu Ser 150 155 160 165 ggt gcg cag gca agt gac atc atg atg ggg ctg cgc gtg atc aag gcg 643 Gly Ala Gln Ala Ser Asp Ile Met Met Gly Leu Arg Val Ile Lys Ala                 170 175 180 atc ggt ggc gag cgt tgg gcc gtg aag act ttt gaa aag gcg tcg cag 691 Ile Gly Gly Glu Arg Trp Ala Val Lys Thr Phe Glu Lys Ala Ser Gln             185 190 195 gca tca gcg aga gcg gcg gtt gat act gca gtt gct tcg ggc aaa gtc 739 Ala Ser Ala Arg Ala Ala Val Asp Thr Ala Val Ala Ser Gly Lys Val         200 205 210 gct ggt att ggt gag ttg tcc att gcg gtg aat ttg gct gcg gtg ttg 787 Ala Gly Ile Gly Glu Leu Ser Ile Ala Val Asn Leu Ala Ala Val Leu     215 220 225 ttg ctt gct ggt tgg cgg gtc acc acg ggg gag ttg ggg cct ggc cag 835 Leu Leu Ala Gly Trp Arg Val Thr Thr Gly Glu Leu Gly Pro Gly Gln 230 235 240 245 ttg atc gca att gtg ggt gtg gcg gtg tat ttg tca gag ccg att cgc 883 Leu Ile Ala Ile Val Gly Val Ala Val Tyr Leu Ser Glu Pro Ile Arg                 250 255 260 ttg ctg agc aac tcg att aat gcc tca gct att gcg cac ggt gca gcg 931 Leu Leu Ser Asn Ser Ile Asn Ala Ser Ala Ile Ala His Gly Ala Ala             265 270 275 gag cgg gtg gct aat ttc tta aac ctc gac gaa tct cag gca cag tac 979 Glu Arg Val Ala Asn Phe Leu Asn Leu Asp Glu Ser Gln Ala Gln Tyr         280 285 290 gaa agc agc gaa aca atc aat gac ggc gaa ttc ctc gtc atc gtg ccc 1027 Glu Ser Ser Glu Thr Ile Asn Asp Gly Glu Phe Leu Val Ile Val Pro     295 300 305 cca gcc agc acg ctt cca cac ggc gac aat atc ttg gct aca cct cat 1075 Pro Ala Ser Thr Leu Pro His Gly Asp Asn Ile Leu Ala Thr Pro His 310 315 320 325 gct gcc gac att ttc gaa ggt acc ttg cgg tca aat att tcc atg aat 1123 Ala Ala Asp Ile Phe Glu Gly Thr Leu Arg Ser Asn Ile Ser Met Asn                 330 335 340 cat gag gac aac gtg cca att gat ccg cag gta att cgc gct tct ggt 1171 His Glu Asp Asn Val Pro Ile Asp Pro Gln Val Ile Arg Ala Ser Gly             345 350 355 ctg act gac atc att gag gtg gac gga ctt gat gcg ccg gtg cgc gat 1219 Leu Thr Asp Ile Ile Glu Val Asp Gly Leu Asp Ala Pro Val Arg Asp         360 365 370 acg gga agc aat tta tcg ggt ggg cag cgt cag cga gtg gct ttg gcc 1267 Thr Gly Ser Asn Leu Ser Gly Gly Gln Arg Gln Arg Val Ala Leu Ala     375 380 385 agg gcg ttg cat gca gac gcg gaa gta ctg gtg ctg atg gat cca acc 1315 Arg Ala Leu His Ala Asp Ala Glu Val Leu Val Leu Met Asp Pro Thr 390 395 400 405 agc gcg gtg gat tca gtg acg gag gtg tct atc gcg cag ggg att aag 1363 Ser Ala Val Asp Ser Val Thr Glu Val Ser Ile Ala Gln Gly Ile Lys                 410 415 420 cag ctg cga gca ggc aaa acc acc att gtg gtg agt tct tcg ccc gcg 1411 Gln Leu Arg Ala Gly Lys Thr Thr Ile Val Val Ser Ser Ser Pro Ala             425 430 435 ttt tac aac ttg gcg gat cgg gtg att tca cat gtc taatttgatg 1457 Phe Tyr Asn Leu Ala Asp Arg Val Ile Ser His Val         440 445 gcatcatcga cac 1470 <210> 284 <211> 449 <212> PRT <213> Corynebacterium glutamicum <400> 284 Met Trp Gln Leu Ser Glu Ala Leu Val Pro Ile Ala Ile Gly Leu Ile   1 5 10 15 Val Asp His Ala Val Leu Thr Lys Asp Leu Arg Arg Leu Val Val Gly              20 25 30 Leu Val Ala Phe Val Val Leu Phe Val Val Leu Ser Phe Ser Asn Arg          35 40 45 Phe Gly Ser Arg Ala Leu Asn Arg Ala Val Asn Phe Glu Ser His Ala      50 55 60 Leu Arg Val Glu Val Ala Asp His Ala Leu Lys Asn Leu Asp Pro Arg  65 70 75 80 Asn Leu Val Pro Gly Glu Val Met Ser Arg Ser Thr Ala Asp Ala Asp                  85 90 95 Ser Ser Thr Arg Ile Phe Gly Gln Ile Gly Thr Gly Val Ser Ala Ala             100 105 110 Thr Gly Phe Leu Gly Ala Ala Thr Tyr Leu Leu Ile Ser Asp Trp Leu         115 120 125 Val Gly Leu Leu Val Leu Val Leu Val Pro Ile Ile Ser Gly Val Val     130 135 140 Ala Leu Ala Ser Lys Gly Ile Ser Lys Arg Ser Val Thr Gln Gln Glu 145 150 155 160 Lys Leu Ala Glu Ser Gly Ala Gln Ala Ser Asp Ile Met Met Gly Leu                 165 170 175 Arg Val Ile Lys Ala Ile Gly Gly Glu Arg Trp Ala Val Lys Thr Phe             180 185 190 Glu Lys Ala Ser Gln Ala Ser Ala Arg Ala Ala Val Asp Thr Ala Val         195 200 205 Ala Ser Gly Lys Val Ala Gly Ile Gly Glu Leu Ser Ile Ala Val Asn     210 215 220 Leu Ala Ala Val Leu Leu Leu Ala Gly Trp Arg Val Thr Thr Gly Glu 225 230 235 240 Leu Gly Pro Gly Gln Leu Ile Ala Ile Val Gly Val Ala Val Tyr Leu                 245 250 255 Ser Glu Pro Ile Arg Leu Leu Ser Asn Ser Ile Asn Ala Ser Ala Ile             260 265 270 Ala His Gly Ala Ala Glu Arg Val Ala Asn Phe Leu Asn Leu Asp Glu         275 280 285 Ser Gln Ala Gln Tyr Glu Ser Ser Glu Thr Ile Asn Asp Gly Glu Phe     290 295 300 Leu Val Ile Val Pro Pro Ala Ser Thr Leu Pro His Gly Asp Asn Ile 305 310 315 320 Leu Ala Thr Pro His Ala Ala Asp Ile Phe Glu Gly Thr Leu Arg Ser                 325 330 335 Asn Ile Ser Met Asn His Glu Asp Asn Val Pro Ile Asp Pro Gln Val             340 345 350 Ile Arg Ala Ser Gly Leu Thr Asp Ile Ile Glu Val Asp Gly Leu Asp         355 360 365 Ala Pro Val Arg Asp Thr Gly Ser Asn Leu Ser Gly Gly Gln Arg Gln     370 375 380 Arg Val Ala Leu Ala Arg Ala Leu His Ala Asp Ala Glu Val Leu Val 385 390 395 400 Leu Met Asp Pro Thr Ser Ala Val Asp Ser Val Thr Glu Val Ser Ile                 405 410 415 Ala Gln Gly Ile Lys Gln Leu Arg Ala Gly Lys Thr Thr Ile Val Val             420 425 430 Ser Ser Ser Pro Ala Phe Tyr Asn Leu Ala Asp Arg Val Ile Ser His         435 440 445 Val      <210> 285 <211> 1368 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1345) <223> RXN01102 <400> 285 attctatggt tgttgggaga gatgacttaa tttggaatca cgggctttaa cacgcgctga 60 cattgagcaa cttcccagca tgtggaaaag cccaggtttc gtg gct gtc ctc gtg 115                                             Val Ala Val Leu Val                                               1 5 gcg gtt gca gca gcg ttc ggc agt tgg tca ctc ctt ctt ccc gtc gta 163 Ala Val Ala Ala Ala Phe Gly Ser Trp Ser Leu Leu Leu Pro Val Val                  10 15 20 ccg cta gcg gtc ctc aac aac ggc gga tca agc gct gtc gcc ggt gcc 211 Pro Leu Ala Val Leu Asn Asn Gly Gly Ser Ser Ala Val Ala Gly Ala              25 30 35 acc act ggc atc ttc atg gca gct aca gtg atc act cag att ttc act 259 Thr Thr Gly Ile Phe Met Ala Ala Thr Val Ile Thr Gln Ile Phe Thr          40 45 50 ccc gct gcg ctg cgg aaa att ggc tac acc cca gtg atg gct ttc gcc 307 Pro Ala Ala Leu Arg Lys Ile Gly Tyr Thr Pro Val Met Ala Phe Ala      55 60 65 gca ttc atg ctg ggt gtg cca gcc atc ggg tac atc ttc agc gtc gag 355 Ala Phe Met Leu Gly Val Pro Ala Ile Gly Tyr Ile Phe Ser Val Glu  70 75 80 85 cca att cca gtg ctg gta gtg tcc gca ctt cga gga att ggg ttc ggt 403 Pro Ile Pro Val Leu Val Val Ser Ala Leu Arg Gly Ile Gly Phe Gly                  90 95 100 gcg ctc acc gtc gca gaa tct gcg ttg gtg gct gaa ctc gtt ccc gta 451 Ala Leu Thr Val Ala Glu Ser Ala Leu Val Ala Glu Leu Val Pro Val             105 110 115 cgc ttc ttg ggc aaa gct tct gga atg ttg ggc gta ttt att ggc ctt 499 Arg Phe Leu Gly Lys Ala Ser Gly Met Leu Gly Val Phe Ile Gly Leu         120 125 130 tcc caa atg ctt ttc ctg cct gcc ggg ttg gcg tta ggt gac caa ttt 547 Ser Gln Met Leu Phe Leu Pro Ala Gly Leu Ala Leu Gly Asp Gln Phe     135 140 145 ggc tac aac gtg gtc tat gtt tta ggt gcc gtt atc gca cta gtt gca 595 Gly Tyr Asn Val Val Tyr Val Leu Gly Ala Val Ile Ala Leu Val Ala 150 155 160 165 gcg gtg atg tgt ctg cgt att ccg cag gtt aag gca gcg gca aag cag 643 Ala Val Met Cys Leu Arg Ile Pro Gln Val Lys Ala Ala Ala Lys Gln                 170 175 180 caa cca cag gtg agc gaa cag gag cgt tct gtt tcc acc tgg aag ttg 691 Gln Pro Gln Val Ser Glu Gln Glu Arg Ser Val Ser Thr Trp Lys Leu             185 190 195 gtg ctg gtt ccc tcc ttg gct gtt acc agt ttg tca atg act ttt ggc 739 Val Leu Val Pro Ser Leu Ala Val Thr Ser Leu Ser Met Thr Phe Gly         200 205 210 gca gtg tct tca ttc ctt cca gct gca gtc att gag tta gat cca gga 787 Ala Val Ser Ser Phe Leu Pro Ala Ala Val Ile Glu Leu Asp Pro Gly     215 220 225 tta ggt gct gca tta gcg ggt att att tta tcc att acc ggt ggt tct 835 Leu Gly Ala Ala Leu Ala Gly Ile Ile Leu Ser Ile Thr Gly Gly Ser 230 235 240 245 tca atg gtg ttc cgc tac ctg tcc ggc gtt atc gct gac cgc cgc ggt 883 Ser Met Val Phe Arg Tyr Leu Ser Gly Val Ile Ala Asp Arg Arg Gly                 250 255 260 gtg cct ggt acc acg atg att cct gct cag atc att ggg ttc tta ggt 931 Val Pro Gly Thr Thr Met Ile Pro Ala Gln Ile Ile Gly Phe Leu Gly             265 270 275 gtc gtt tta atc acc gtc aca atc ttc caa ggc tgg tcc gtg tgg ctt 979 Val Val Leu Ile Thr Val Thr Ile Phe Gln Gly Trp Ser Val Trp Leu         280 285 290 ttg att ata ggt gca gtg atg ttt ggt ggt gct ttt ggc atg gtg caa 1027 Leu Ile Ile Gly Ala Val Met Phe Gly Gly Ala Phe Gly Met Val Gln     295 300 305 aac gaa gcg ttg ctt tca atg ttt ttc cgg ctt cct cgc act aga gtc 1075 Asn Glu Ala Leu Leu Ser Met Phe Phe Arg Leu Pro Arg Thr Arg Val 310 315 320 325 tcc gaa gcc tcc gcc atc tgg aat atc gcc ttt gat tcg gga aca gga 1123 Ser Glu Ala Ser Ala Ile Trp Asn Ile Ala Phe Asp Ser Gly Thr Gly                 330 335 340 atc gga agc ttc ctc ctt ggc ata gtt gcc gca tcg ctt gct tac agt 1171 Ile Gly Ser Phe Leu Leu Gly Ile Val Ala Ala Ser Leu Ala Tyr Ser             345 350 355 ggt gct ttt ggt tcc gga gcc gtg gtg att ttg ttt gga atc gtt ttg 1219 Gly Ala Phe Gly Ser Gly Ala Val Val Ile Leu Phe Gly Ile Val Leu         360 365 370 acc acc gcc gat cga atc att ggg cgg cac cgc att act gaa tac aac 1267 Thr Thr Ala Asp Arg Ile Ile Gly Arg His Arg Ile Thr Glu Tyr Asn     375 380 385 aac acc cgc gcg cgt ttg cgc cag gtg cca gtc gct cgg cgt gca gtg 1315 Asn Thr Arg Ala Arg Leu Arg Gln Val Pro Val Ala Arg Arg Ala Val 390 395 400 405 caa ggg ctg cgc aac agg cgc aaa gat cgc taaaacgctt ttcgacgcca 1365 Gln Gly Leu Arg Asn Arg Arg Lys Asp Arg                 410 415 ccc 1368 <210> 286 <211> 415 <212> PRT <213> Corynebacterium glutamicum <400> 286 Val Ala Val Leu Val Ala Val Ala Ala Ala Phe Gly Ser Trp Ser Leu   1 5 10 15 Leu Leu Pro Val Val Pro Leu Ala Val Leu Asn Asn Gly Gly Ser Ser              20 25 30 Ala Val Ala Gly Ala Thr Thr Gly Ile Phe Met Ala Ala Thr Val Ile          35 40 45 Thr Gln Ile Phe Thr Pro Ala Ala Leu Arg Lys Ile Gly Tyr Thr Pro      50 55 60 Val Met Ala Phe Ala Ala Phe Met Leu Gly Val Pro Ala Ile Gly Tyr  65 70 75 80 Ile Phe Ser Val Glu Pro Ile Pro Val Leu Val Val Ser Ala Leu Arg                  85 90 95 Gly Ile Gly Phe Gly Ala Leu Thr Val Ala Glu Ser Ala Leu Val Ala             100 105 110 Glu Leu Val Pro Val Arg Phe Leu Gly Lys Ala Ser Gly Met Leu Gly         115 120 125 Val Phe Ile Gly Leu Ser Gln Met Leu Phe Leu Pro Ala Gly Leu Ala     130 135 140 Leu Gly Asp Gln Phe Gly Tyr Asn Val Val Tyr Val Leu Gly Ala Val 145 150 155 160 Ile Ala Leu Val Ala Ala Val Met Cys Leu Arg Ile Pro Gln Val Lys                 165 170 175 Ala Ala Ala Lys Gln Gln Pro Gln Val Ser Glu Gln Glu Arg Ser Val             180 185 190 Ser Thr Trp Lys Leu Val Leu Val Pro Ser Leu Ala Val Thr Ser Leu         195 200 205 Ser Met Thr Phe Gly Ala Val Ser Ser Phe Leu Pro Ala Ala Val Ile     210 215 220 Glu Leu Asp Pro Gly Leu Gly Ala Ala Leu Ala Gly Ile Ile Leu Ser 225 230 235 240 Ile Thr Gly Gly Ser Ser Met Val Phe Arg Tyr Leu Ser Gly Val Ile                 245 250 255 Ala Asp Arg Arg Gly Val Pro Gly Thr Thr Met Ile Pro Ala Gln Ile             260 265 270 Ile Gly Phe Leu Gly Val Val Leu Ile Thr Val Thr Ile Phe Gln Gly         275 280 285 Trp Ser Val Trp Leu Leu Ile Ile Gly Ala Val Met Phe Gly Gly Ala     290 295 300 Phe Gly Met Val Gln Asn Glu Ala Leu Leu Ser Met Phe Phe Arg Leu 305 310 315 320 Pro Arg Thr Arg Val Ser Glu Ala Ser Ala Ile Trp Asn Ile Ala Phe                 325 330 335 Asp Ser Gly Thr Gly Ile Gly Ser Phe Leu Leu Gly Ile Val Ala Ala             340 345 350 Ser Leu Ala Tyr Ser Gly Ala Phe Gly Ser Gly Ala Val Val Ile Leu         355 360 365 Phe Gly Ile Val Leu Thr Thr Ala Asp Arg Ile Ile Gly Arg His Arg     370 375 380 Ile Thr Glu Tyr Asn Asn Thr Arg Ala Arg Leu Arg Gln Val Pro Val 385 390 395 400 Ala Arg Arg Ala Val Gln Gly Leu Arg Asn Arg Arg Lys Asp Arg                 405 410 415 <210> 287 <211> 348 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (325) <223> RXN00788 <400> 287 cgcatccctc tagttttcca tcacctcaat gaacggcgct aactccggtt cattgcgcaa 60 ttgatccagc actgcttgca gtgaggcctc attagttggc atg gcc tcc tcc atc 115                                             Met Ala Ser Ser Ile                                               1 5 aac atc gga gtg ttc aac ctt gga aat gct gtt gct gcc tgg ctt gct 163 Asn Ile Gly Val Phe Asn Leu Gly Asn Ala Val Ala Ala Trp Leu Ala                  10 15 20 ggt gca acc atc acc act tcc ctt gga ctc aca tca gcc gga tta gtt 211 Gly Ala Thr Ile Thr Thr Ser Leu Gly Leu Thr Ser Ala Gly Leu Val              25 30 35 ggc ggt ttg atg acg tcc ctc gga cta gtg ttg gcc atc gtg gct gtg 259 Gly Gly Leu Met Thr Ser Leu Gly Leu Val Leu Ala Ile Val Ala Val          40 45 50 gtt ttg cgt cga aaa gcg caa ggc acc caa gcg acc atc agc gtt gtg 307 Val Leu Arg Arg Lys Ala Gln Gly Thr Gln Ala Thr Ile Ser Val Val      55 60 65 gag cac cag ccc gcc caa taaataattt ctctcttcta att 348 Glu His Gln Pro Ala Gln  70 75 <210> 288 <211> 75 <212> PRT <213> Corynebacterium glutamicum <400> 288 Met Ala Ser Ser Ile Asn Ile Gly Val Phe Asn Leu Gly Asn Ala Val   1 5 10 15 Ala Ala Trp Leu Ala Gly Ala Thr Ile Thr Thr Ser Leu Gly Leu Thr              20 25 30 Ser Ala Gly Leu Val Gly Gly Leu Met Thr Ser Leu Gly Leu Val Leu          35 40 45 Ala Ile Val Ala Val Val Leu Arg Arg Lys Ala Gln Gly Thr Gln Ala      50 55 60 Thr Ile Ser Val Val Glu His Gln Pro Ala Gln  65 70 75 <210> 289 <211> 1764 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (101) .. (1741) <223> RXN02119 <400> 289 ttcggtccgc tctggcaaaa atggctggct gccacctcgg cgcagcagct taagggctgg 60 gcttaaattg cttgtcgacg cctagtgcca caatggagac atg acc gaa aca ctt 115                                             Met Thr Glu Thr Leu                                               1 5 gtg gtg aat ggc ctt gca ggc ggc tat ggg cac cgc aca tta ttt aac 163 Val Val Asn Gly Leu Ala Gly Gly Tyr Gly His Arg Thr Leu Phe Asn                  10 15 20 gat gtg aat ctc acc gta gct gcc ggc gat gtc gtg ggc gtt gtc ggc 211 Asp Val Asn Leu Thr Val Ala Ala Gly Asp Val Val Gly Val Val Gly              25 30 35 gtc aat ggc gct ggt aaa tcc aca ttt cta aaa att ctg gcg ggc gtg 259 Val Asn Gly Ala Gly Lys Ser Thr Phe Leu Lys Ile Leu Ala Gly Val          40 45 50 gaa aag cca ctg gct gga act atc gcg ctt tcg cca gcc gat gct ttt 307 Glu Lys Pro Leu Ala Gly Thr Ile Ala Leu Ser Pro Ala Asp Ala Phe      55 60 65 gtg ggc tac ttg cca cag gaa cac acc cgc acg tct gga gag acg atc 355 Val Gly Tyr Leu Pro Gln Glu His Thr Arg Thr Ser Gly Glu Thr Ile  70 75 80 85 gca gtt tac att gct cgt cga acc ggc tgc caa gct gca aca act gcc 403 Ala Val Tyr Ile Ala Arg Arg Thr Gly Cys Gln Ala Ala Thr Thr Ala                  90 95 100 atg gat gac acc gcc gaa gcg ttt ggt gcg gat cca gac aac gct gcc 451 Met Asp Asp Thr Ala Glu Ala Phe Gly Ala Asp Pro Asp Asn Ala Ala             105 110 115 ttg gcc gat gca tac gcc gag gcg ctg gat cgg tgg atg gcc agt ggc 499 Leu Ala Asp Ala Tyr Ala Glu Ala Leu Asp Arg Trp Met Ala Ser Gly         120 125 130 gca gcc gat ttg gat gaa cgc atc ccc atc gtg ctc gct gat ttg ggc 547 Ala Ala Asp Leu Asp Glu Arg Ile Pro Ile Val Leu Ala Asp Leu Gly     135 140 145 ttt gag ctt ccc acc tcg acg ctg atg gaa gga ctt tca ggc ggg cag 595 Phe Glu Leu Pro Thr Ser Thr Leu Met Glu Gly Leu Ser Gly Gly Gln 150 155 160 165 gca gcc cgg gtc ggg ctg gcg gcg tta ctg ttg tca cgt ttt gac att 643 Ala Ala Arg Val Gly Leu Ala Ala Leu Leu Leu Ser Arg Phe Asp Ile                 170 175 180 gtg ctt ctc gac gag ccc acc aac gat ttg gat ctc gac ggt ctt gag 691 Val Leu Leu Asp Glu Pro Thr Asn Asp Leu Asp Leu Asp Gly Leu Glu             185 190 195 caa ctg gag aat ttt gtt cag ggg ctt cgc ggg gga gtc gta ctg gtc 739 Gln Leu Glu Asn Phe Val Gln Gly Leu Arg Gly Gly Val Val Leu Val         200 205 210 agc cat gat cgt gag ttt ctt tcc agg tgt gtg acc act gtg ctg gaa 787 Ser His Asp Arg Glu Phe Leu Ser Arg Cys Val Thr Thr Val Leu Glu     215 220 225 ctc gat ctg cac caa aat tcc cac cat gtt tat ggc ggt gga tat gat 835 Leu Asp Leu His Gln Asn Ser His His Val Tyr Gly Gly Gly Tyr Asp 230 235 240 245 tcc tac ctt gag gaa cgc gca gtg cta cgc cag cac gcc cgt gac caa 883 Ser Tyr Leu Glu Glu Arg Ala Val Leu Arg Gln His Ala Arg Asp Gln                 250 255 260 tat gag gaa ttt gcg gaa aag aag aag gac ctt gtg gca cgt gct cga 931 Tyr Glu Glu Phe Ala Glu Lys Lys Lys Asp Leu Val Ala Arg Ala Arg             265 270 275 acg cag cgt gaa tgg tct agt cac ggt gtc cgc aat gct att aaa cgt 979 Thr Gln Arg Glu Trp Ser Ser His Gly Val Arg Asn Ala Ile Lys Arg         280 285 290 gca cct gac aac gac aaa ctt cgg aag aaa gcc gct gcg gaa tcc agt 1027 Ala Pro Asp Asn Asp Lys Leu Arg Lys Lys Ala Ala Ala Glu Ser Ser     295 300 305 gaa aag cag gct caa aaa gtc cgc cag atg gaa agc cgc atc gct cgg 1075 Glu Lys Gln Ala Gln Lys Val Arg Gln Met Glu Ser Arg Ile Ala Arg 310 315 320 325 tta gaa gaa gtt gaa gag cca cgt aaa gaa tgg aaa ctg cag ttc agc 1123 Leu Glu Glu Val Glu Glu Pro Arg Lys Glu Trp Lys Leu Gln Phe Ser                 330 335 340 gtc ggt aag gcg tcg cgg tca agt tct gtt gtt tcc acg ttg aat gat 1171 Val Gly Lys Ala Ser Arg Ser Ser Ser Val Val Ser Thr Leu Asn Asp             345 350 355 gca agc ttc acc caa ggc gat ttc acc ttg gga cca gta tcc atc caa 1219 Ala Ser Phe Thr Gln Gly Asp Phe Thr Leu Gly Pro Val Ser Ile Gln         360 365 370 gta aat gct ggc gat cgc att ggc atc aca gga ccc aac ggt gct ggt 1267 Val Asn Ala Gly Asp Arg Ile Gly Ile Thr Gly Pro Asn Gly Ala Gly     375 380 385 aaa tcc aca ttg ctg cgc gga cta ttg gga aac caa gaa ccc acc agc 1315 Lys Ser Thr Leu Leu Arg Gly Leu Leu Gly Asn Gln Glu Pro Thr Ser 390 395 400 405 ggt act gcc acg atg ggc acg agc gtg gcg atc gga gaa atc gat cag 1363 Gly Thr Ala Thr Met Gly Thr Ser Val Ala Ile Gly Glu Ile Asp Gln                 410 415 420 gca cga gcg tta ctt gat cca cag ttg cca ctg att tct gcg ttt gaa 1411 Ala Arg Ala Leu Leu Asp Pro Gln Leu Pro Leu Ile Ser Ala Phe Glu             425 430 435 aag cat gtt cca gac tta ccg atc agt gag gtg cgc aca ctg ctc gcg 1459 Lys His Val Pro Asp Leu Pro Ile Ser Glu Val Arg Thr Leu Leu Ala         440 445 450 aaa ttt ggg ctg aat gat aat cat gtg gaa cgg gac gtc gaa aag cta 1507 Lys Phe Gly Leu Asn Asp Asn His Val Glu Arg Asp Val Glu Lys Leu     455 460 465 tct cct ggc gag cgc acg cgc gcc gga ctt gcg ctg cta cag gtg cgg 1555 Ser Pro Gly Glu Arg Thr Arg Ala Gly Leu Ala Leu Leu Gln Val Arg 470 475 480 485 ggc gtc aac gtg ctt gtt ctt gat gag ccc acc aac cac ctt gac ctg 1603 Gly Val Asn Val Leu Val Leu Asp Glu Pro Thr Asn His Leu Asp Leu                 490 495 500 gag gcc atc gag caa ttg gag caa gcg ttg gcc tcg tat gat ggt gtg 1651 Glu Ala Ile Glu Gln Leu Glu Gln Ala Leu Ala Ser Tyr Asp Gly Val             505 510 515 ttg ctg ctg gtc acg cac gat cgt cgc atg ttg gac gct gtg cag acc 1699 Leu Leu Leu Val Thr His Asp Arg Arg Met Leu Asp Ala Val Gln Thr         520 525 530 aat cgt cgt tgg cat gtc gag gct ggc gaa gtt agg gag cta 1741 Asn Arg Arg Trp His Val Glu Ala Gly Glu Val Arg Glu Leu     535 540 545 taaccgtttc cgtattgatg cca 1764 <210> 290 <211> 547 <212> PRT <213> Corynebacterium glutamicum <400> 290 Met Thr Glu Thr Leu Val Val Asn Gly Leu Ala Gly Gly Tyr Gly His   1 5 10 15 Arg Thr Leu Phe Asn Asp Val Asn Leu Thr Val Ala Ala Gly Asp Val              20 25 30 Val Gly Val Val Gly Val Asn Gly Ala Gly Lys Ser Thr Phe Leu Lys          35 40 45 Ile Leu Ala Gly Val Glu Lys Pro Leu Ala Gly Thr Ile Ala Leu Ser      50 55 60 Pro Ala Asp Ala Phe Val Gly Tyr Leu Pro Gln Glu His Thr Arg Thr  65 70 75 80 Ser Gly Glu Thr Ile Ala Val Tyr Ile Ala Arg Arg Thr Gly Cys Gln                  85 90 95 Ala Ala Thr Thr Ala Met Asp Asp Thr Ala Glu Ala Phe Gly Ala Asp             100 105 110 Pro Asp Asn Ala Ala Leu Ala Asp Ala Tyr Ala Glu Ala Leu Asp Arg         115 120 125 Trp Met Ala Ser Gly Ala Ala Asp Leu Asp Glu Arg Ile Pro Ile Val     130 135 140 Leu Ala Asp Leu Gly Phe Glu Leu Pro Thr Ser Thr Leu Met Glu Gly 145 150 155 160 Leu Ser Gly Gly Gln Ala Ala Arg Val Gly Leu Ala Ala Leu Leu Leu                 165 170 175 Ser Arg Phe Asp Ile Val Leu Leu Asp Glu Pro Thr Asn Asp Leu Asp             180 185 190 Leu Asp Gly Leu Glu Gln Leu Glu Asn Phe Val Gln Gly Leu Arg Gly         195 200 205 Gly Val Val Leu Val Ser His Asp Arg Glu Phe Leu Ser Arg Cys Val     210 215 220 Thr Thr Val Leu Glu Leu Asp Leu His Gln Asn Ser His His Val Tyr 225 230 235 240 Gly Gly Gly Tyr Asp Ser Tyr Leu Glu Glu Arg Ala Val Leu Arg Gln                 245 250 255 His Ala Arg Asp Gln Tyr Glu Glu Phe Ala Glu Lys Lys Lys Asp Leu             260 265 270 Val Ala Arg Ala Arg Thr Gln Arg Glu Trp Ser Ser His Gly Val Arg         275 280 285 Asn Ala Ile Lys Arg Ala Pro Asp Asn Asp Lys Leu Arg Lys Lys Ala     290 295 300 Ala Ala Glu Ser Ser Glu Lys Gln Ala Gln Lys Val Arg Gln Met Glu 305 310 315 320 Ser Arg Ile Ala Arg Leu Glu Glu Val Glu Glu Pro Arg Lys Glu Trp                 325 330 335 Lys Leu Gln Phe Ser Val Gly Lys Ala Ser Arg Ser Ser Ser Val Val             340 345 350 Ser Thr Leu Asn Asp Ala Ser Phe Thr Gln Gly Asp Phe Thr Leu Gly         355 360 365 Pro Val Ser Ile Gln Val Asn Ala Gly Asp Arg Ile Gly Ile Thr Gly     370 375 380 Pro Asn Gly Ala Gly Lys Ser Thr Leu Leu Arg Gly Leu Leu Gly Asn 385 390 395 400 Gln Glu Pro Thr Ser Gly Thr Ala Thr Met Gly Thr Ser Val Ala Ile                 405 410 415 Gly Glu Ile Asp Gln Ala Arg Ala Leu Leu Asp Pro Gln Leu Pro Leu             420 425 430 Ile Ser Ala Phe Glu Lys His Val Pro Asp Leu Pro Ile Ser Glu Val         435 440 445 Arg Thr Leu Leu Ala Lys Phe Gly Leu Asn Asp Asn His Val Glu Arg     450 455 460 Asp Val Glu Lys Leu Ser Pro Gly Glu Arg Thr Arg Ala Gly Leu Ala 465 470 475 480 Leu Leu Gln Val Arg Gly Val Asn Val Leu Val Leu Asp Glu Pro Thr                 485 490 495 Asn His Leu Asp Leu Glu Ala Ile Glu Gln Leu Glu Gln Ala Leu Ala             500 505 510 Ser Tyr Asp Gly Val Leu Leu Leu Val Thr His Asp Arg Arg Met Leu         515 520 525 Asp Ala Val Gln Thr Asn Arg Arg Trp His Val Glu Ala Gly Glu Val     530 535 540 Arg Glu Leu 545 <210> 291 <211> 1638 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1615) <223> RXN01605 <400> 291 attgaagtca gcgccgagga agccgcgagg gaatcagacg acgggcacct actacttcgg 60 cattgaaatt ccgaagaact tcagcgattc tattgccagc gtg acc agc gat tca 115                                             Val Thr Ser Asp Ser                                               1 5 ccc gcg cca gca acc gtc aac gcg gta ttc aac aac agc aac ggc ttc 163 Pro Ala Pro Ala Thr Val Asn Ala Val Phe Asn Asn Ser Asn Gly Phe                  10 15 20 att gcc tcc atg ctg ggc aac cag gtg gtc aac act gtt gtg gag acc 211 Ile Ala Ser Met Leu Gly Asn Gln Val Val Asn Thr Val Val Glu Thr              25 30 35 atg gac acg gaa ttc ggc gtc cgc att gtg gat aac atg ctc gtc ggt 259 Met Asp Thr Glu Phe Gly Val Arg Ile Val Asp Asn Met Leu Val Gly          40 45 50 ttc tcc acc ttg ggc gac ggc atg aac caa gcc gcc gaa ggt gcc act 307 Phe Ser Thr Leu Gly Asp Gly Met Asn Gln Ala Ala Glu Gly Ala Thr      55 60 65 acg ctc agc gat ggc gtc ggt tcc gcc aac gac ggt gca gtt cag ctt 355 Thr Leu Ser Asp Gly Val Gly Ser Ala Asn Asp Gly Ala Val Gln Leu  70 75 80 85 gcc gac ggc gcg gtc acc ctg cgc gac ggc atc gca agt gcc aat gag 403 Ala Asp Gly Ala Val Thr Leu Arg Asp Gly Ile Ala Ser Ala Asn Glu                  90 95 100 ggt gcg caa tcg ctt gcc gac ggc gcc agc cag ctc gac acc ggc ctc 451 Gly Ala Gln Ser Leu Ala Asp Gly Ala Ser Gln Leu Asp Thr Gly Leu             105 110 115 ggc tcc gcg gct aca ggc agc caa acg ctc gcc gac ggt cta tcc agc 499 Gly Ser Ala Ala Thr Gly Ser Gln Thr Leu Ala Asp Gly Leu Ser Ser         120 125 130 ctg tct gcg ggc acc gcc caa cta ggc caa ggc gca acc cag gtt tca 547 Leu Ser Ala Gly Thr Ala Gln Leu Gly Gln Gly Ala Thr Gln Val Ser     135 140 145 gat ggc gtg ggc caa ctt gtc gac caa gta gca cca ctg acc gcc tat 595 Asp Gly Val Gly Gln Leu Val Asp Gln Val Ala Pro Leu Thr Ala Tyr 150 155 160 165 gtt cca gac atc aac tct cag ttg atc acc ctg cgc gac ggc gca gcc 643 Val Pro Asp Ile Asn Ser Gln Leu Ile Thr Leu Arg Asp Gly Ala Ala                 170 175 180 acc att gcc tct gaa cta tct gat ccc tcc agc acc tac cgc tcc ggc 691 Thr Ile Ala Ser Glu Leu Ser Asp Pro Ser Ser Thr Tyr Arg Ser Gly             185 190 195 gtg gac tcc gct gtg agc gca tcc cag caa cta gca gcc ggc ctg caa 739 Val Asp Ser Ala Val Ser Ala Ser Gln Gln Leu Ala Ala Gly Leu Gln         200 205 210 acc ctg aaa gac gga tcc agc caa ctc agc atc ggt gca cgc acc ctc 787 Thr Leu Lys Asp Gly Ser Ser Gln Leu Ser Ile Gly Ala Arg Thr Leu     215 220 225 gct gat ggc acc agc caa ttg gcc gca ggt tcc gaa cag cta gtt gtt 835 Ala Asp Gly Thr Ser Gln Leu Ala Ala Gly Ser Glu Gln Leu Val Val 230 235 240 245 ggc gca caa gca ctg cgc gac ggc acc gtc cag ctt gat gaa ggc tcc 883 Gly Ala Gln Ala Leu Arg Asp Gly Thr Val Gln Leu Asp Glu Gly Ser                 250 255 260 agc gaa ctc gcc ctc aaa ctc acc gac ggc gca agc caa gta cca acc 931 Ser Glu Leu Ala Leu Lys Leu Thr Asp Gly Ala Ser Gln Val Pro Thr             265 270 275 ttc gct gac ggc gca gac acc acc atc gca acc cca gtt gaa aca gaa 979 Phe Ala Asp Gly Ala Asp Thr Thr Ile Ala Thr Pro Val Glu Thr Glu         280 285 290 caa gca gga gac acc aca ccg ctc ttc ggt att ggt ctc gca cca ttc 1027 Gln Ala Gly Asp Thr Thr Pro Leu Phe Gly Ile Gly Leu Ala Pro Phe     295 300 305 ttc atg gct gtc ggc ctg ttc atg gga gca acc gtt gcc tgg atg atc 1075 Phe Met Ala Val Gly Leu Phe Met Gly Ala Thr Val Ala Trp Met Ile 310 315 320 325 ctg cac cca atc agt cgc cgc gca ctc gac tcc cgc atg gga ggc ttc 1123 Leu His Pro Ile Ser Arg Arg Ala Leu Asp Ser Arg Met Gly Gly Phe                 330 335 340 cga ggc acc ctg gca agc tac ctt cca tca aca gtc tta ggc ctt ggc 1171 Arg Gly Thr Leu Ala Ser Tyr Leu Pro Ser Thr Val Leu Gly Leu Gly             345 350 355 caa gca acc atc atg tgg gca gta ctg tac ttc ctg ctc gac ctc aat 1219 Gln Ala Thr Ile Met Trp Ala Val Leu Tyr Phe Leu Leu Asp Leu Asn         360 365 370 cca gct cac cca gct gga ctg tgg atg gcg atg gtc gcc atc tca tgg 1267 Pro Ala His Pro Ala Gly Leu Trp Met Ala Met Val Ala Ile Ser Trp     375 380 385 gta ttc atc tcc att acc cat atg ttc aac aac gtg gca gga ccc tcc 1315 Val Phe Ile Ser Ile Thr His Met Phe Asn Asn Val Ala Gly Pro Ser 390 395 400 405 gca ggc cgt gtg ctg tcc atc gtg atg atg tcc ttc cag cta gtc tcc 1363 Ala Gly Arg Val Leu Ser Ile Val Met Met Ser Phe Gln Leu Val Ser                 410 415 420 tcc ggt ggc cta tac cca cca gaa acc cag cca gca ttc ttc cac tgg 1411 Ser Gly Gly Leu Tyr Pro Pro Glu Thr Gln Pro Ala Phe Phe His Trp             425 430 435 ttc cac acc tac gac ccg atc acc tac gca gtc aac ctc gtg cgc caa 1459 Phe His Thr Tyr Asp Pro Ile Thr Tyr Ala Val Asn Leu Val Arg Gln         440 445 450 atg atc ttc aac gaa acc cca tcc aac gac cca cgc ttc ata caa gca 1507 Met Ile Phe Asn Glu Thr Pro Ser Asn Asp Pro Arg Phe Ile Gln Ala     455 460 465 atc tgg gta ctg ctc ttc atc tgg gca ctg atg ctc gcc atc tcc acc 1555 Ile Trp Val Leu Leu Phe Ile Trp Ala Leu Met Leu Ala Ile Ser Thr 470 475 480 485 ctg gcg aat aga aca aac aag gtt ctt cgc atg aag gac tac cac cca 1603 Leu Ala Asn Arg Thr Asn Lys Val Leu Arg Met Lys Asp Tyr His Pro                 490 495 500 gaa ctg aag gtc taaaagcttt tcccgcccgg ttc 1638 Glu leu lys val             505 <210> 292 <211> 505 <212> PRT <213> Corynebacterium glutamicum <400> 292 Val Thr Ser Asp Ser Pro Ala Pro Ala Thr Val Asn Ala Val Phe Asn   1 5 10 15 Asn Ser Asn Gly Phe Ile Ala Ser Met Leu Gly Asn Gln Val Val Asn              20 25 30 Thr Val Val Glu Thr Met Asp Thr Glu Phe Gly Val Arg Ile Val Asp          35 40 45 Asn Met Leu Val Gly Phe Ser Thr Leu Gly Asp Gly Met Asn Gln Ala      50 55 60 Ala Glu Gly Ala Thr Thr Leu Ser Asp Gly Val Gly Ser Ala Asn Asp  65 70 75 80 Gly Ala Val Gln Leu Ala Asp Gly Ala Val Thr Leu Arg Asp Gly Ile                  85 90 95 Ala Ser Ala Asn Glu Gly Ala Gln Ser Leu Ala Asp Gly Ala Ser Gln             100 105 110 Leu Asp Thr Gly Leu Gly Ser Ala Ala Thr Gly Ser Gln Thr Leu Ala         115 120 125 Asp Gly Leu Ser Ser Leu Ser Ala Gly Thr Ala Gln Leu Gly Gln Gly     130 135 140 Ala Thr Gln Val Ser Asp Gly Val Gly Gln Leu Val Asp Gln Val Ala 145 150 155 160 Pro Leu Thr Ala Tyr Val Pro Asp Ile Asn Ser Gln Leu Ile Thr Leu                 165 170 175 Arg Asp Gly Ala Ala Thr Ile Ala Ser Glu Leu Ser Asp Pro Ser Ser             180 185 190 Thr Tyr Arg Ser Gly Val Asp Ser Ala Val Ser Ala Ser Gln Gln Leu         195 200 205 Ala Ala Gly Leu Gln Thr Leu Lys Asp Gly Ser Ser Gln Leu Ser Ile     210 215 220 Gly Ala Arg Thr Leu Ala Asp Gly Thr Ser Gln Leu Ala Ala Gly Ser 225 230 235 240 Glu Gln Leu Val Val Gly Ala Gln Ala Leu Arg Asp Gly Thr Val Gln                 245 250 255 Leu Asp Glu Gly Ser Ser Glu Leu Ala Leu Lys Leu Thr Asp Gly Ala             260 265 270 Ser Gln Val Pro Thr Phe Ala Asp Gly Ala Asp Thr Thr Ile Ala Thr         275 280 285 Pro Val Glu Thr Glu Gln Ala Gly Asp Thr Thr Pro Leu Phe Gly Ile     290 295 300 Gly Leu Ala Pro Phe Phe Met Ala Val Gly Leu Phe Met Gly Ala Thr 305 310 315 320 Val Ala Trp Met Ile Leu His Pro Ile Ser Arg Arg Ala Leu Asp Ser                 325 330 335 Arg Met Gly Gly Phe Arg Gly Thr Leu Ala Ser Tyr Leu Pro Ser Thr             340 345 350 Val Leu Gly Leu Gly Gln Ala Thr Ile Met Trp Ala Val Leu Tyr Phe         355 360 365 Leu Leu Asp Leu Asn Pro Ala His Pro Ala Gly Leu Trp Met Ala Met     370 375 380 Val Ala Ile Ser Trp Val Phe Ile Ser Ile Thr His Met Phe Asn Asn 385 390 395 400 Val Ala Gly Pro Ser Ala Gly Arg Val Leu Ser Ile Val Met Met Ser                 405 410 415 Phe Gln Leu Val Ser Ser Gly Gly Leu Tyr Pro Pro Glu Thr Gln Pro             420 425 430 Ala Phe Phe His Trp Phe His Thr Tyr Asp Pro Ile Thr Tyr Ala Val         435 440 445 Asn Leu Val Arg Gln Met Ile Phe Asn Glu Thr Pro Ser Asn Asp Pro     450 455 460 Arg Phe Ile Gln Ala Ile Trp Val Leu Leu Phe Ile Trp Ala Leu Met 465 470 475 480 Leu Ala Ile Ser Thr Leu Ala Asn Arg Thr Asn Lys Val Leu Arg Met                 485 490 495 Lys Asp Tyr His Pro Glu Leu Lys Val             500 505 <210> 293 <211> 664 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (664) <223> RXN01091 <400> 293 tcgtcggaaa aactggcgat actattgata acgggcatgg gttcaccttc gataaagcac 60 ggtctatgct ggactatcgc cttttgacac gagtatcgca atg gtc ccg aac aca 115                                             Met val pro asn thr                                               1 5 gtc ctt atc cat gac gaa acc gcc gat ctg gcg acg cag atc cag cgg 163 Val Leu Ile His Asp Glu Thr Ala Asp Leu Ala Thr Gln Ile Gln Arg                  10 15 20 ctg gaa cat atc atg gcg tgc ctg cgc gat ccg gtc agc gga tgc ccg 211 Leu Glu His Ile Met Ala Cys Leu Arg Asp Pro Val Ser Gly Cys Pro              25 30 35 tgg gat att gaa cag acc ttt gcc agc atc gcg ccc cac acg att gag 259 Trp Asp Ile Glu Gln Thr Phe Ala Ser Ile Ala Pro His Thr Ile Glu          40 45 50 gaa ggc tac gag gtt gcc gac gcc atc gcg cag gaa gac tgg ccc gag 307 Glu Gly Tyr Glu Val Ala Asp Ala Ile Ala Gln Glu Asp Trp Pro Glu      55 60 65 cta cgc ggc gag ttg ggc gat ttg ctg ttt cag acc gtg ttt cac gcc 355 Leu Arg Gly Glu Leu Gly Asp Leu Leu Phe Gln Thr Val Phe His Ala  70 75 80 85 caa atg gcg cgc gag gca ggc cat ttc gct ttg gtt gac gtg gtg aag 403 Gln Met Ala Arg Glu Ala Gly His Phe Ala Leu Val Asp Val Val Lys                  90 95 100 gca att tcg gac aag atg gtt ttg cgc cat ccg cac gtg ttc ggc gcg 451 Ala Ile Ser Asp Lys Met Val Leu Arg His Pro His Val Phe Gly Ala             105 110 115 cag tcg aac gcg aaa tcc gcc gac cag cag gtg gaa gat tgg gaa gtc 499 Gln Ser Asn Ala Lys Ser Ala Asp Gln Gln Val Glu Asp Trp Glu Val         120 125 130 atc aag gcg ccc gag cgc gcg ggc aaa gcg caa aag ggc gtt ttg gat 547 Ile Lys Ala Pro Glu Arg Ala Gly Lys Ala Gln Lys Gly Val Leu Asp     135 140 145 ggc gtc gcg ctg gga ctg cct gcc ctg atg cgc gcg acg aag ctg caa 595 Gly Val Ala Leu Gly Leu Pro Ala Leu Met Arg Ala Thr Lys Leu Gln 150 155 160 165 aac aac gcc gcg cgc gtt ggg ttt gat tgg ccc gac att ggg cag gta 643 Asn Asn Ala Ala Arg Val Gly Phe Asp Trp Pro Asp Ile Gly Gln Val                 170 175 180 ctt gga aag gtg acc gag gaa 664 Leu Gly Lys Val Thr Glu Glu             185 <210> 294 <211> 188 <212> PRT <213> Corynebacterium glutamicum <400> 294 Met Val Pro Asn Thr Val Leu Ile His Asp Glu Thr Ala Asp Leu Ala   1 5 10 15 Thr Gln Ile Gln Arg Leu Glu His Ile Met Ala Cys Leu Arg Asp Pro              20 25 30 Val Ser Gly Cys Pro Trp Asp Ile Glu Gln Thr Phe Ala Ser Ile Ala          35 40 45 Pro His Thr Ile Glu Glu Gly Tyr Glu Val Ala Asp Ala Ile Ala Gln      50 55 60 Glu Asp Trp Pro Glu Leu Arg Gly Glu Leu Gly Asp Leu Leu Phe Gln  65 70 75 80 Thr Val Phe His Ala Gln Met Ala Arg Glu Ala Gly His Phe Ala Leu                  85 90 95 Val Asp Val Val Lys Ala Ile Ser Asp Lys Met Val Leu Arg His Pro             100 105 110 His Val Phe Gly Ala Gln Ser Asn Ala Lys Ser Ala Asp Gln Gln Val         115 120 125 Glu Asp Trp Glu Val Ile Lys Ala Pro Glu Arg Ala Gly Lys Ala Gln     130 135 140 Lys Gly Val Leu Asp Gly Val Ala Leu Gly Leu Pro Ala Leu Met Arg 145 150 155 160 Ala Thr Lys Leu Gln Asn Asn Ala Ala Arg Val Gly Phe Asp Trp Pro                 165 170 175 Asp Ile Gly Gln Val Leu Gly Lys Val Thr Glu Glu             180 185 <210> 295 <211> 357 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (334) <223> RXS02979 <400> 295 ctaggtcaag gaacttcact cggctagtcc ttagactcaa atgtgttcag acaaacactg 60 gcaccgtaag gcacgaaagt taccgaaagg actggttccc atg acc gcc cca aac 115                                             Met thr ala pro asn                                               1 5 act ctc aag caa aca act ctt cgc tct gat gag ttc tct tgc cca tcc 163 Thr Leu Lys Gln Thr Thr Leu Arg Ser Asp Glu Phe Ser Cys Pro Ser                  10 15 20 tgt gtc tcc aag att gaa aac aaa ttg aat gga ttg gat ggc gtc gac 211 Cys Val Ser Lys Ile Glu Asn Lys Leu Asn Gly Leu Asp Gly Val Asp              25 30 35 aat gca gag gtg aag ttc tcc tcc gga aga atc ctt gtt gat cac gac 259 Asn Ala Glu Val Lys Phe Ser Ser Gly Arg Ile Leu Val Asp His Asp          40 45 50 ccc agc aag gtc tct atc aag gat cta gtc gct gca gtc gca gag gtt 307 Pro Ser Lys Val Ser Ile Lys Asp Leu Val Ala Ala Val Ala Glu Val      55 60 65 ggc tac acc gca aag cca tca gca atc taaaactctc agttagacca 354 Gly Tyr Thr Ala Lys Pro Ser Ala Ile  70 75 tta 357 <210> 296 <211> 78 <212> PRT <213> Corynebacterium glutamicum <400> 296 Met Thr Ala Pro Asn Thr Leu Lys Gln Thr Thr Leu Arg Ser Asp Glu   1 5 10 15 Phe Ser Cys Pro Ser Cys Val Ser Lys Ile Glu Asn Lys Leu Asn Gly              20 25 30 Leu Asp Gly Val Asp Asn Ala Glu Val Lys Phe Ser Ser Gly Arg Ile          35 40 45 Leu Val Asp His Asp Pro Ser Lys Val Ser Ile Lys Asp Leu Val Ala      50 55 60 Ala Val Ala Glu Val Gly Tyr Thr Ala Lys Pro Ser Ala Ile  65 70 75 <210> 297 <211> 357 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (334) <223> RXS02987 <400> 297 gttgtttgat ccaggtcaag gaattaaccc ggaaaggacc gtatctttaa aggtgcaagc 60 acaggaacat gacgataaaa gatgaaagga cctggttacg atg acc gcc ccc gcc 115                                             Met thr ala pro ala                                               1 5 acg ctg aag aac acc acc ttg cgc tct gat gag ttc acc tgt ccg agc 163 Thr Leu Lys Asn Thr Thr Leu Arg Ser Asp Glu Phe Thr Cys Pro Ser                  10 15 20 tgt gtc gcc aag atc gaa aac aag ctg aat ggt ttg gac ggc gtg gag 211 Cys Val Ala Lys Ile Glu Asn Lys Leu Asn Gly Leu Asp Gly Val Glu              25 30 35 aat gcg gag gtg aag ttc tcc tcc gga cgc atc ctg atc acc cac gac 259 Asn Ala Glu Val Lys Phe Ser Ser Gly Arg Ile Leu Ile Thr His Asp          40 45 50 cca cag aag gtc tcc gta cgt gac ctg gtc acc gcg gta gcc gag gtc 307 Pro Gln Lys Val Ser Val Arg Asp Leu Val Thr Ala Val Ala Glu Val      55 60 65 ggt tac acc gcc aag ccg tcg gcg atc tgacgcactc ccgaccccac 354 Gly Tyr Thr Ala Lys Pro Ser Ala Ile  70 75 aag 357 <210> 298 <211> 78 <212> PRT <213> Corynebacterium glutamicum <400> 298 Met Thr Ala Pro Ala Thr Leu Lys Asn Thr Thr Leu Arg Ser Asp Glu   1 5 10 15 Phe Thr Cys Pro Ser Cys Val Ala Lys Ile Glu Asn Lys Leu Asn Gly              20 25 30 Leu Asp Gly Val Glu Asn Ala Glu Val Lys Phe Ser Ser Gly Arg Ile          35 40 45 Leu Ile Thr His Asp Pro Gln Lys Val Ser Val Arg Asp Leu Val Thr      50 55 60 Ala Val Ala Glu Val Gly Tyr Thr Ala Lys Pro Ser Ala Ile  65 70 75 <210> 299 <211> 492 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (469) <223> RXS03095 <400> 299 aacgcctcaa ttagtgccag accttgccga ccgcagacca aacttcacca tttcaaacca 60 tccctagcca caacaacggc agttgtgcaa tgatctgcgt atg aat gca gat aag 115                                             Met Asn Ala Asp Lys                                               1 5 aaa atg tgc gga atg aac ccg gat agc caa tac gtc gaa ctt gcc gtc 163 Lys Met Cys Gly Met Asn Pro Asp Ser Gln Tyr Val Glu Leu Ala Val                  10 15 20 gaa gtt ttc gga ctc ctc gcg gac gcc act cga gtt cgc atc atc ttg 211 Glu Val Phe Gly Leu Leu Ala Asp Ala Thr Arg Val Arg Ile Ile Leu              25 30 35 gca ctt cga aac agt ggt gaa ctt tcc gta aac cac ctc gcg gac atc 259 Ala Leu Arg Asn Ser Gly Glu Leu Ser Val Asn His Leu Ala Asp Ile          40 45 50 gtc gat aaa tcc ccc gca gca gtt tcc caa cac ctc gcc cgg ctg cgc 307 Val Asp Lys Ser Pro Ala Ala Val Ser Gln His Leu Ala Arg Leu Arg      55 60 65 atg gcc cga atc gtg tcc acc cgt caa gaa ggt caa cga gtt ttc tac 355 Met Ala Arg Ile Val Ser Thr Arg Gln Glu Gly Gln Arg Val Phe Tyr  70 75 80 85 aaa ctc acc aat gaa cac gca tca cag cta gtc tcc gac gct att ttt 403 Lys Leu Thr Asn Glu His Ala Ser Gln Leu Val Ser Asp Ala Ile Phe                  90 95 100 cag gcg gaa cac acc att gcg gac ggc cag act ccc cca cac cac cac 451 Gln Ala Glu His Thr Ile Ala Asp Gly Gln Thr Pro Pro His His His             105 110 115 cga gaa cga gaa caa tca tgaccaccca cagtcaccaa gaa 492 Arg Glu Arg Glu Gln Ser         120 <210> 300 <211> 123 <212> PRT <213> Corynebacterium glutamicum <400> 300 Met Asn Ala Asp Lys Lys Met Cys Gly Met Asn Pro Asp Ser Gln Tyr   1 5 10 15 Val Glu Leu Ala Val Glu Val Phe Gly Leu Leu Ala Asp Ala Thr Arg              20 25 30 Val Arg Ile Ile Leu Ala Leu Arg Asn Ser Gly Glu Leu Ser Val Asn          35 40 45 His Leu Ala Asp Ile Val Asp Lys Ser Pro Ala Ala Val Ser Gln His      50 55 60 Leu Ala Arg Leu Arg Met Ala Arg Ile Val Ser Thr Arg Gln Glu Gly  65 70 75 80 Gln Arg Val Phe Tyr Lys Leu Thr Asn Glu His Ala Ser Gln Leu Val                  85 90 95 Ser Asp Ala Ile Phe Gln Ala Glu His Thr Ile Ala Asp Gly Gln Thr             100 105 110 Pro Pro His His His Arg Glu Arg Glu Gln Ser         115 120 <210> 301 <211> 10 <212> PRT <213> Corynebacterium glutamicum <400> 301 Ala Pro Ala Leu Gly Pro Thr Leu Ser Gly   1 5 10 <210> 302 <211> 10 <212> PRT <213> Corynebacterium glutamicum <220> <221> VARIANT <222> 2-4, 7, 8 X223 = any amino acid <400> 302 Gly Xaa Xaa Xaa Gly Pro Xaa Xaa Gly Gly   1 5 10 <210> 303 <211> 20 <212> DNA <213> Corynebacterium glutamicum <400> 303 ctccaggatt gctccgaagg 20 <210> 304 <211> 20 <212> DNA <213> Corynebacterium glutamicum <400> 304 cacagtggtt gaccactggc 20 <210> 305 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: primer <400> 305 ggaaacagta tgaccatg 18 <210> 306 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: primer <400> 306 gtaaaacgac ggccagt 17

Claims (36)

delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete A method for producing lysine, comprising culturing a host cell transformed with an expression vector comprising an isolated nucleic acid molecule encoding a polypeptide consisting of the amino acid sequence set forth in SEQ ID NO: 2. The method of claim 23, further comprising recovering lysine from the culture. The method of claim 23, wherein the cells are included in Corynebacterium or Brevibacterium. The method of claim 23, wherein the cells are Corynebacterium glutamicum, Corynebacterium herculis , Corynebacterium lilium , Corynebacterium acetoacidophyllum ( Coynebacterium acetoacidophilum), Corynebacterium acetonitrile glutamicum (Corynebacterium acetoglutamicum), Corynebacterium acetonitrile pilrum (Corynebacterium acetophilum), Corynebacterium ammoniagenes to Ness (Corynebacterium ammoniagenes), Corynebacterium Fujio kense (Corynebacterium fujiokense), Corynebacterium nitrilophilus , Brevibacterium ammoniagenes , Brevibacterium butanicum , Brevibacterium divaricatum , Brevibacterium divaricatum Non- bacterium flavum ( Brevibacterium hea ), Brevibacterium hea lii ), Brevibacterium ketoglutamicum , Brevibacterium ketosoreductum , Brevibacterium lactofermentum , Brevibacterium linens ( Brevibacterium linens ) , And Brevibacterium paraffinolyticum . delete delete delete delete delete delete delete delete delete delete