KR100870882B1 - SJP microorganism in Paenibacillus - Google Patents

Abstract

The present invention is not only excellent in fermentation and anti-corruption of organic matter, but also insecticidal effect of maggots and pests and bactericidal effect of plant, Candida genus, Kazatania genus, Bacillus genus, Camobacterium which have antibiotic replacement effect of livestock. Providing probiotics with odor prevention and pesticide disinfection effect using 11 kinds of SJP microorganisms in the genus, improving environment, and providing feed additives having antibiotic replacement effect, odor, flies prevention and growth promoting effect. The aim is to provide fermented foods and fermented herbal medicines that promote digestion, fart, and defecation of feces and promote health.

SJP microorganism, Lactobacillus, Brevivacillus, Penivacillus, Camobacterium, Cultivation method, Odor prevention, Insecticide, Sterilization, Fermented Ginseng.

Description

SJP microorganism in Paenibacillus

The present invention provides an SJP microorganism having effects such as odor prevention and sterilization insecticide at the waste treatment site, growth promotion of animals and prevention of odor and flies.

Research is being actively conducted to solve the odor of organic waste, but so far, not only the damage of residents due to the odors, but also the flies that occur in the livestock industry such as pig farms, pig farms, Korean cattle breeders, and poultry farms is not alone. . This fact proves the effectiveness of the odor-resolving microorganisms developed so far.

Or the biggest problem for the livestock industry is the abuse of antibiotics for the development of odors and flies. There have been many studies to solve this problem, but it has not been solved.

The present invention aims to improve the environment and contribute to the development of the livestock industry by providing SJP microorganisms having effects such as odor prevention and sterilization of insects at waste treatment sites, promotion of livestock growth and prevention of odors and flies.

In detailing the source of SJP microorganisms, the inventor (SeJoonPark) is an idea for developing a natural insecticide for insect pests that damages crops in 2005. In the toxic plants such as choo, rich, monarch, baekbuja, mulberry, and deadwood, The insecticidal test of pests such as cabbage worm and caterpillar stink bug that damages crops by extracting toxic substances by hydrolysis method was conducted. However, when stink bugs and adult insects were not killed, it was impossible to use them as natural insecticides. Therefore, if they were sprayed on microorganisms periodically while searching for other uses, they could find microorganisms with high toxicity and viability among surviving microorganisms. I came up with the idea that you can expect an effect.

Based on the same idea, pile up various soils such as soil, octopus, foliar soil, fecal soil, and periodically spray for about 6 months with the natural insecticide and salt-blended sulfur, and collect this soil to compost 100 tons of food waste per day in Siheung, Gyeonggi-do. As a result of using odor prevention in fermentation facilities, all 12 kinds of odors regulated by Korea's odor prevention law such as ammonia were 0.00ppm as shown in the odor measurement report of the Gyeonggi-do Institute of Health and Environment.

The food waste composting facility has a container for separating and storing foreign materials (vinyl bags, pig heads, fish, etc.) that cannot be recycled and transported to the incinerator every 15 days. In addition to the unpleasant odor of the container, maggots were ugly to the outside as if they sprayed a few pieces of rice every morning. Here, the soil was sprayed in order to determine the odor-preventing effect so that no single fly larvae occurred. In addition, 12 to 13 tons of compost was produced per day in the plant composting fermentation device, but after using the soil was reduced to 5 to 6 tons. Before and after use, the moisture content was 65 ~ 70% before use, but after use, it was reduced to 45 ~ 48% and the volume was reduced to 1/3.

In order to reaffirm the odor prevention effect and the maggot insecticidal effect, the soil was sprinkled by fists in 13 conventional toilets, and all of the odors and maggots were killed. In order to confirm the effect caused by the microorganism, the compost (sample) fermented with the soil (microorganism) for 10 days, the compost fermented for 30 days, and the compost (sample) fermented food waste without using the soil Microorganisms were isolated from the control sample. As a result, 24 kinds of microorganisms were not found in the control sample.

Effects of Agriculture, Livestock and Fisheries

It is used in agriculture, livestock, and fisheries without the use of antibiotics to promote growth, reduce mortality, odor prevention, flies prevention, rapid fermentation of livestock, and suppress the phytopathogens of the produced compost. It is possible to improve the competitiveness of livestock and fisheries industry by providing the effect of. In addition, it has been possible to cleanly solve the problem of pathogens resistant to humans by livestock and aquatic products that have been seriously concerned.

Environmental effect

It contributes to organic agriculture by rapidly fermenting organic wastes such as human waste and livestock, such as food waste, food waste, and odors, and supplying them to the soil, thereby contributing to organic farming. It became.

Maggot larvae insecticide and odor prevention test

Ten species of microorganisms were randomly selected among the 24 species to determine the ability to prevent flies and odor. Decayed fish and chicken brought into the food waste dump. 5 liters of pork in two 20ℓ barrels were left at room temperature for 4 days to produce maggots and dispersed in 10 containers.The number of maggots was dispersed to at least 500 ~ 1,000 grains and contained 10 kinds of SJP microbial liquid 10cc Was mixed with 100cc of water and sprayed, respectively, and when maggots were killed, five species of SJP6728AF1, SJP6722AF2, SJP6730AF3, SJP6722A5, and SJP6735B6 were killed as shown in Table 1. Even though the SJP microorganism treatment group in which maggots were killed was left for 3 days at room temperature in August when flies were blown, the freshness did not change as shown in Table 1 and there was no odor. There was no odor after 6 days and the freshness of the fish was maintained at 70% or more as shown in FIG. The meat is decayed and discarded as trash, and it was left for 4 days at room temperature in summer to make maggots again. It represents excellence. The antiseptic action and odor prevention mechanism is believed to occur depending on the ability to prevent or extinguish the activity of the decaying bacteria.

Food waste fermentation test

6 pieces of food waste 65% of food waste brought into the compost fermenter were put in 10 liters of 20 liter buckets. Next, 20 cc of the six SJP microbial liquids were sprayed on a pail and shaken to mix evenly and kept warm at 40 to 50 ° C. And 3 days after the odor was investigated by the sensory method continuously as shown in Table 1.

Food waste waste odor prevention test

Prepare 6 containers of 10 liters of food waste (BOD 100,000ppm) with vibrating odor, and then mix 6 kinds of SJP microbial liquids with 10cc of 100cc of water and spray 2 of each strain on 20 buckets. Odor change was measured. After 1 hour, all five SJP microorganisms of the present invention had the odor disappeared by 95% or more, and after 90 minutes, the smell of alcohol began to appear. In order to investigate how much alcohol odor occurs in alcohol concentration, the waste water was put into a distilled water device equipped with a cooling device as shown in FIG. In addition, to determine whether the smell of the alcohol odor is prevented and the smell of alcohol until a period of time left to stand at room temperature for 2 months and examined from time to time. As a result, the smell of alcohol gradually decreased, but the odor did not occur with the corruption stopped.

Table 1. Fly larvae insecticidal effect and fermentation, odor prevention effect test

 ◆ Fly Larva Insect Experiment Microorganism name Insecticide time Odor effect and maggot status SJP6728AF1 100% 4  Three days after the killing of maggots SJP6735B6 100% 4  12 hours after death, it disintegrates and becomes jelly. Odorless 5 days old meat half preservation SJP66722A5 50% 4  Odorless, maggot regeneration vigorous activity after 2 days SJP6730AF3 70% 4  12 hours later, half-decomposed fish, odorless 24 hours SJP6729AF2 100% 2  Three days after the dead, five days after the complete decomposition of maggots ◆ Food waste fermentation experiment ( 40 ℃ warming for 3 days after inoculation with 1% water content 65% food waste) Microorganism name Deodorant effect term Sensory test result SJP6728AF1 100% 3 days Odorless SJP6729AF2 100% 3 days Odorless SJP6730AF3 100% 3 days Odorless SJP6735B6 100% 3 days Odorless SJP66722A5 100% 3 days Odorless SJP6731B1 100% 3 days Odorless ◆ Food waste waste odor prevention test ( measurement of odor change after inoculation of culture spawn in waste water) Microorganism name Odor effect time effect Alcohol concentration SJP6728AF1 90% 90 minutes  1 hour 30 minutes End of odor Alcohol smell 8% SJP6730AF3 90% 90 minutes  1 hour 30 minutes End of odor Alcohol smell 7.5% SJP6729AF2 90% 90 minutes  1 hour 30 minutes End of odor Alcohol smell 7.5%

SJP Antimicrobial and Antifungal Activity Test of Microorganisms

The antimicrobial and antifungal activity test against plant pathogens of SJP microorganism of the present invention was commissioned to the Plant Pathology Department of the Ministry of Agriculture, Science and Technology, as shown in Table 2 below.

Table 2 SJP antibacterial and anti-fungal activity of the test microorganism (laboratory)

  - Germ Antimicrobial activity A5 AF1 AF2 AF3 B1 B6 Agrobacterium vitis + - - - - ++ Clavibacter michiganensis subsp, michiganensis + + - - - ++ Pectobacterium carotovorum subsp . carotovorum ( malignancy ) + - - - - + Xanthomonas campestris pv. campestris(Black rot germ) + - - - - +  - mold Colletotrichum gloeosporide s + - + + +++ Fusarium oxysporum + - - + +++ Phytophthora capsici (Station germs) - - - - + Rhizoctonia solani + + + + - ++ Sclerotinia sclerotiorum + + + + - ++  -Degree of activity:-no effect + effect ++ effect

Saccharomyces excellent in the above experiments exiguus) in SJP6728AF1, SJP6729AF2, Candida fructus fructus ) Lactobacillus et al., a novel strain of SJP6730AF3 paraplantarum ) genus SJP6722A5 and Paenibacillus polymyxa genus SJP6735B6 strain, bacillus badius ) genus SJP6731B1 strain was isolated and identified the analysis results are presented in Figure 1 and these strains were deposited in the Korea microbial conservation center and given the accession number as shown in Table 3, the patent application to the Republic of Korea was granted patent No. 0536456.

Table 3. Deposited strain name and accession number of SJP microorganism

Deposited strain Deposit number SJP6728AF1 KCCM-10675P SJP6729AF2 KCCM-10677P SJP6730AF3 KCCM-10679P SJP66722A5 KCCM-10676P SJP6735B6 KCCM-10678P SJP6731B1 KCCM-10680P

Food waste collection container odor prevention experiment

The 6 kinds of SJP before and after the collection of food waste in 1,200 collection containers distributed in Siheung, Gyeonggi-do, with the idea that preventing the occurrence of odor in food waste collection containers can solve the odor generated in transport vehicles and food waste pretreatment facilities. As a result of spraying 20 ~ 50cc of microbial probiotics, there was no odor in the collection container as well as the vehicle and pretreatment facility. In addition, even if food waste is not collected for 3 to 5 days (up to 10 days), odors and maggots do not occur, and food waste collection cars have no odors, making the urban environment pleasant. In addition, it is possible to solve the problem of unwilling to leave the container in front of the house, the odor pain of the residents to block the nose and use the container, and the nymph phenomenon of food waste or manure treatment facility. Siheung-Gyeonggi-do, Gyeonggi-do, which uses SJP microorganisms, awarded the citation to the inventors as shown in FIG. 6 and proved the results of use and effectiveness of FIG.

In the summer, the cleaner who collects food waste and manure was not able to enter the restaurant and suffered a lot of odor problems from the lifts and homes while returning home, but the sanitation workers were freed from the odor. The fact is that the sanitation agency suffering from the stench sent a thank you letter as shown in FIG. Food waste collection containers, as well as collection vehicles and treatment plants do not cause odors to avoid the pain of odors. The present inventors applied for such effect under the title of ' Invention prevention method of organic waste treatment using microorganisms ' and was granted a patent as Patent No. 10-0581738 (registration date 2006.05.12).

Secondary SJP Microbial Isolation and Testing

Meanwhile, as a result of investigating and storing the patented SJP microorganisms, the first 24 isolates of the shaker were stored in a 3 ° C. refrigerator for 90 days. It was done. The purpose of the re-separation after 90 days has been achieved with the expectation that if there is no viable nutrient and there is a microorganism that survives in a low temperature environment, it can be very useful practically. By this separation, the first and second isolates were isolated from 32 species. Of the 10 species used in the first experiment, the effects were identified. Thus, except for the second experiment, 14 untested and 8 secondary isolates were added. The experiment to verify the maggot insecticide and bactericidal and fungicidal effect was carried out as follows.

Maggots etc Harmful pest  insecticide

5 liters of decayed fish, whole chicken, and pork were brought into the food waste treatment plant, and 5 liters of each were placed.These were left for 4 days at room temperature with flies to produce maggots and dispersed in 22 experimental zones. At least 500 to 1,000 dogs. After mixing 10cc of each microbial liquid incubated in a medium made of organic materials such as rice bran, wheat bran and soybean meal in 100cc of water, they were sprayed into 22 containers containing maggots. In 2-6 hours maggots were killed 50-100%. After two days, maggots began to develop again in the Bacillus, and odor again occurred after three days. However, although the freshness of fish and whole chicken did not change even after 3 days had elapsed, the freshness changed more than 70% after 6 days, but the odor did not occur, and it was similar to the first isolated yeast. However, according to the components of the culture medium, the pesticidal effect was significantly different.

Since the activity of microorganisms differs depending on the culture medium as in the maggot insecticide test, a medium of various components was prepared and carried out over 100 times, and the maggot insecticide test results shown in Table 4 presented the average data.

Organic Fermentation Experiment

The organic fermentation test was carried out in the same manner as the first test method, and 20 more food wastes having a water content of 65% and 10 liters were put in a bucket. Next, 20cc of the culture medium in which each of the 22 microorganisms isolated were sprayed and stirred in 22 pails, and then heated to maintain 40 to 50 ° C. After 3 days, the odor was continuously measured using the sensory method and odor meter. As a result, it was confirmed that the microorganisms that had excellent maggot insecticides also had excellent fermentation ability. Analytical methods were used to measure the volume reduction of organic matter and at the same time, to determine the degree of odor change and the degree of odor change and decrease by using sensory and odor measuring instruments. The results showed that eleven species were excluded from identification because of their low odor prevention effect and maggoticidal effect. In addition, a number of organic fermentation experiments were conducted, including food waste 80-ton composting facility and livestock fermentation test in Hanwoo cattle house. The evaluation was performed by measuring the odor using the sensory method and the odor meter using 10 people and evaluating the volume reduction by the actual measurement method.

Food Waste Waste Odor Prevention Experiment

This experiment was conducted in the same way as the food waste waste odor prevention test in the 1st test for 11 species that showed excellent effectiveness except low fermentation capacity or low pesticide activity in the experiment. Was carried out.

Prepare 11 experiments containing 10 liters of food waste wastewater (BOD 100,000ppm) in a 20ℓ bucket and one control for the odor vibrating.Spray 5cc of SJP microbial liquid into each of 11 test zones and change the odor. Measured by comparing with one control. After 1 hour, 100% of the odor disappeared, and after 90 minutes, the smell of alcohol began to develop in the experimental group inoculated with the yeast strain. The specific gravity was measured by distilling the liquid from the experimental group with the smell of alcohol. Yeast species was 6-8%, similar to the first experiment. In addition, the acidity of the yeast strain PH3.7 Lactobacillus PH4.1 Penivacillus PH 4.2 showed a strong acidity. All of the experimental zones subjected to the above experiments were left in a compost fermentation apparatus maintained at 35 ° C. to 40 ° C. for at least one month, but no odor occurred.

In order to investigate the mechanism that does not cause odor, 30 liters of food waste was left in the fermentation fermentation chamber for 7 days to completely rot, and then made three experiments by dividing the sample made to generate maximum odor such as ammonia. The density of decaying bacteria such as hydrogen sulfide was investigated by dilution plate smear method. As a result, about 3-15 × 10 ⁸ bacteria were detected per 1cc, and the SJP6840AF4 strain, SJP6841L2 strain, and strain culture solution 10cc were added to each of the three experiments and stirred, and after 2 hours, the density of decaying bacteria such as ammonia was diluted. After a review to each reputation malbeop SJP6840AF4 it strains experiment phrase 1cc about ² ~ 5 × 10 2 SJP6841L2 strain 1cc about 2 ~ 5 × 10 ³ SJP6719B3 strains of bacteria of about 2 ~ 5 × 10 ³ was detected per 1cc per sugar.

Table 4. Effect of Maggot Insect and Food Waste Fermentation and Food Waste Waste Odor Prevention Test

 ◆ Maggot Insect Experiment Microorganism name Insecticide Death time SJP6840AF4, SJP6844AF5, SJP6841L2, SJP6734B4, 100% 2  SJP6726AF6, SJP6843AF7, 100% 3 SJP6720L3, SJP6723L4, SJP6742L5, 60-70% 6  SJP6732B2, SJP6719B3, 40-50% 6 ◆ Food waste fermentation test ( water content 65% food waste in 20 L bucket, 40 ℃ heating for 3 days after inoculation) Microorganism Types Fermentation effect Fermentation period SJP6840AF4, SJP6844AF5, SJP6726AF6, SJP6843AF7 100% 3 days SJP6841L2, SJP6720L3, SJP6723L4, SJP6742L5, SJP6732B2, SJP6719B3, SJP6734B4, 80% 3 days ◆ Food Waste Waste Odor Prevention Experiment Microorganism name Odor prevention effect time Alcohol concentration SJP6840AF4, SJP6844AF5, SJP6726AF6, SJP6843AF7 100% 60 minutes  6 ~ 8 SJP6841L2, SJP6720L3, SJP6723L4, SJP6742L5, SJP6732B2, SJP6719B3, SJP6734B4 90% 90 minutes  3 ~ 5

SJP Antimicrobial activity of microorganisms and Antifungal  Active experiment

In order to investigate the antimicrobial activity of bacteria and fungi that damage the crops of SJP microorganisms of the present invention was commissioned by the Department of Plant Pathology, Department of Agriculture and Biological Sciences. The results are shown in Table 5, and the antimicrobial activity photograph is shown in Figure 9 as a method to prove the experiment.

Table 5 of the SJP microorganisms antimicrobial and anti-fungal activity test (laboratory)

   Classification designation Abbreviation   Germ Agtobacterium vifis Ag Clavibacter michiganensts subsp, michiganensis Cl Erwinia carotovora subsp, carotovora Er Xanthomonas campestris pv campestris Xa   mold Colletotrichum gloeosporioides Pepper Anthrax Co Fusarium oxysporum (lobe disease) Fu Phytophthora capsici (Pepper Plague) Ph Rhizoctonia solani (Riceworm) Rh Sclerotinia sclerotiorum (mycosis) Sc SJP microbial antimicrobial activity SJP Microbiology Ag Cl Er Xa Co Fu Ph Rh Sc Remarks SJP6840AF4 + - - - - - + - SJP6844AF5 + - - - - - + - + SJP6726AF6 - - - - - - + - + SJP6843AF7 - - - + - - - - - SJP6841L2 ++ ++ ++ + +++ +++ +++ ++ ++ SJP6720L3 + + + + ++ + ++ + ++ SJP6723L4 + - - + ++ + - - + SJP6742L5 + + + + ++ + + ++ + SJP6732B2 + + + + + + - + + SJP6719B3 + + ++ + + +++ ++ + - SJP6734B4 ++ - + ++ - ++ +++ + ++ Activity level view:-No effect + Effect drug ++ Effect +++

Similar strain  exam

Judging the practicality of the SJP microorganisms by the above test, it was identified by the Korea Microorganism Conservation Center (KCCM) and identified as SJP6840AF4 strain Candida zeylanoides genus as shown in FIG . SJP6844AF5 strain is a genus of Kazachstania aerobia. SJP6726AF6 strain is a genus of Candida humilis . SJP6843AF7 strain was identified as yeast in the genus Candida zeylanoides . SJP6841L2 is a genus of Lactobacillus casei , SJP6720L3 is a genus of Lactobacillus brevis , and SJP6732L4 is a genus of Lactobacillus ctreum . SJP6742L5 strain is a genus of Camobacterium maltaromaticum . The SJP6732B2 strain was identified as Paenibacillus lactis , the SJP6719B3 strain was identified as Paenibacillus sp, and the SJP6734B4 strain was named as Brevibacillus borsterensis . The identified strain was deposited in the Korea Microbial Preservation Center (KCCM). The results of the deposit are summarized in the table below.
Strain name Depositary Institution Accession number Deposit date SJP6719B3 Korea Microorganism Conservation Center KCCM-10727P 2005.12.29 SJP6734B4 Korea Microorganism Conservation Center KCCM-10728P 2005.12.29 SJP6841L2 Korea Microorganism Conservation Center KCCM-10729P 2005.12.29 SJP6720L3 Korea Microorganism Conservation Center KCCM-10730P 2005.12.29 SJP6723L4 Korea Microorganism Conservation Center KCCM-10731P 2005.12.29 SJP6742L5 Korea Microorganism Conservation Center KCCM-10732P 2005.12.29

The similar strains closest to the genus of SJP microorganisms were purchased from microbial banks such as Korea Microorganism Conservation Center (KCCM) and cultured in the medium used for the culture of SJP microorganisms of the present invention, and maggot insect test, odor prevention test, and organic fermentation test were performed. . As for the test method, the food waste fermentation test and the food waste waste odor prevention test were performed in the same manner as the SJP microbial test method, and the odor reduction effect was shown in Table 6 in terms of reduction value.

In the test results of similar strains, maggot insecticidal test showed 30% mortality after 12 hours, but maggot again rebounded after only 10 hours. Also, the odor prevention effect was significantly lower than that of SJP microorganisms. In addition, despite the cultivation in the same SJP microorganisms and the medium, there were many microorganisms with different odors of similar species and SJP microorganisms, and found that the odor change was rapid even in the culture medium storage.

Table 6 . Similar strain test results

 ◆ Maggot insect test Pseudomicroorganisms Insecticide Death time Candida zeylanoides , Candida humilis . 00% 12 Lactobacillus casei,, Lactobacillus brevis Camobacterium . maltaromaticum Brevibacillus borsterensis 10-20% 9 Paenibacillus sp, Paenibacillus lactis Lactobacillus ctreum 20-30% 6 ◆ Food waste fermentation test (water content 65% food waste in a 20ℓ bucket, 40 ℃ heating for 3 days after inoculation of the culture spawn) Similar microorganism types Fermentation effect Fermentation period Candida zeylanoides . Candida humilis . Kazachstania aerobia . 50% 3 days Camobacterium . maltaromaticum Brevibacillus borsterensis 30% Lactobacillus casei,, Lactobacillus brevis Paenibacillus sp, Paenibacillus lactis Lactobacillus ctreum 10%

Intestinal insect insect test

Mosquito larvae insect pest test was carried out as follows. About 50 worms were prepared in a bottle filled with about 80 worms in a puddle filled with worms, and 10 cc of the 11 kinds of SJP microorganisms were added and observed. After 6 hours, the mosquito larvae of the test cells injected with SJP6841L2 and SJP6734B4 strains started to die, resulting in 100% killing after 3 hours, followed by 4 hours after SJP6732B2 strains started to die It was confirmed that all of the killing effect. In addition, the Bacillus strain added test was less active compared to the control group without microorganisms, but SJP6840AF4, SJP6844AF5, SJP6726AF6, SJP6843AF7, yeast strains showed a rather active activity.

Antimicrobial and Antifungal  Activity analysis

Analysis of the antimicrobial and antifungal activity of SJP microorganisms against plant pathogens of the present invention was commissioned by the Department of Plant Pathology, Department of Biology, Department of Biology, National Academy of Agricultural Science and Technology, RDA. .

Antibacterial and anti-fungal activity test in Table 7 SJP microorganisms (laboratory tests)

   Classification designation Abbreviation   Germ Agtobacterium vifis ( roots disease) Ag Clavibacter michiganensts subsp, michiganensis Cl Erwinia carotovora subsp, carotovora (Soft bug) Er Xanthomonas campestris pv campestris (Black rot germ) Xa   mold Colletotrichum gloeosporioides (anthrax) Co Fusarium oxysporum (seed disease) Fu Phytophthora capsici Blight Bacteria Ph Rhizoctonia solani (Shrunk bacillus) Rh Sclerotinia sclerotiorum Sc SJP microbial antimicrobial activity SJP Microbiology Ag Cl Er Xa Co Fu Ph Rh Sc Remarks SJP6840AF4 + - - - - - + - SJP6844AF5 + - - - - - + - + SJP6726AF6 - - - - - - + - + SJP6843AF7 - - - + - - - - - SJP6841L2 ++ ++ ++ + +++ +++ +++ ++ ++ SJP6720L3 + + + + ++ + ++ + ++ SJP6723L4 + - - + ++ + - - + SJP6742L5 + + + + ++ + + ++ + SJP6732B2 + + + + + + - + + SJP6719B3 + + ++ + + +++ ++ + - SJP6734B4 ++ - + ++ - ++ +++ + ++ Activity level view:-No effect + Effect drug ++ Effect +++

SJP Microbial Fermentation Compost Analysis

Analysis of microbial density of compost fermented food waste with SJP microorganisms of the present invention to the Department of Plant Pathology, Department of Biology, Department of Biology, National Academy of Agricultural Science and Technology, RDA, Table 8 and Microbial Review Report (Enforcement, Plant Pathology Division-384 ( 2006.02.07)).

Table 8. Microbial Density by Compost Sample (Unit: X 10 ⁶ CFU / g)

Sample Name Germ mold leaven Specific heat treatment Heat treatment (70 ℃ 10 minutes) SJP microbial compost (10 days fermentation) 56.0 1.0 0.07 0.32 SJP microbial compost (30 days fermentation) 12.3 2.4 0 0 Control Compost (effective 30 days) 1.1 0.1 0.73 0

Title: Microorganism Review Report

1. Review Results

* The number of microorganisms in fermented compost was about 12 to 56 times higher than that of control compost, and the density of mold was relatively low, but the density of yeast was higher in fermentation compost for 10 days.

* Microorganisms detected in compost were found to be dominated by bacteria of the genus Bacillus and yeast.

* Some microorganisms with odor reduction and fly larvae insecticidal effect were identified.

* Some microorganisms with antibacterial activity against phytopathogens have been identified.

* Some microorganisms that promote plant growth have been identified.

2. Opinion

* Microorganisms that are effective in reducing odor may be used for food waste and livestock manure after precise testing in the field.

* It is thought that microorganisms that have antimicrobial activity, promote plant growth and insecticide effect on fly larvae can be used as pest control agents.

In another test, SJP6840AF4 strain, SJP6844AF5 strain, SJP6726AF6 strain, SJP6841L2 strain, SJP6720L3 strain, SJP6732B2 strain, SJP6719B3 strain, SJP6743B4 strain, and SJP6728B1 strain and SJP6728AF1 and SJP6730AF3 were cultured in SJP6730AF3 for 3 days at 35 ° C. Organic fermentation and maggot insecticidal effects were tested. It was confirmed that the effect was somewhat superior to the effect used as a single bacteria.

In addition, as a result of analyzing the types and densities of microorganisms in compost fermenting food waste using SJP microorganisms and microorganisms in general compost, in addition to the 39 species isolated in the test subjects There were 12 more beneficial bacteria that were beneficial to crops, and there were 51 species in total, and about 1 to 50 × 10 ⁸ were present in 1 g. However, it was confirmed that 23 kinds of microorganisms, such as filamentous fungi, exist in the general compost and about 1 to 15 × 10 ^{4 in} 1 g. These tests identified the antagonistic ability of SJP microorganisms, and concluded that the SJP microorganisms of the present invention can also be used in combination and cultured with conventional beneficial bacteria.

In addition, the characteristics of the SJP microorganisms firstly isolated and the strains of the present invention isolated secondly have different effects depending on the medium composition in the ability to prevent odor, bactericidal disinfection, fermentation and anti-corruption. For example, inoculated with 11 strains of SJP microorganisms of the present invention (SJP6728AF4, SJP6844AF5, SJP6726AF6, SJP6841L2, SJP6720L3, SJP6732B2, SJP6719B3, SJP6840B4) in a medium prepared only with rice bran, SJP6726 AF4, SJP6726 AF4, SJP673 AFAF6, Strain was the most effective. However, strains of SJP6844AF5, SJP6841L2, and SJP6743B4 were the best in the medium prepared only with bran. As such, it was found that the superiority changed depending on the medium. The fermentation ability, the odor prevention ability, and the insecticidal ability of the yeast and bacillus of the first isolated SJP microorganism and the second isolated microorganism of the present invention were evaluated to be similar. It is excellent, but it is effective to use 1 ~ 2 species of yeast genus and 1 ~ 2 species of genus Bacillus. Therefore, these strains were deposited in the Korea microbial preservation center (KCCM), the accession number and SJP microbial names are shown in Table 9 and same.

Table 9. Deposited Strain Names and Deposit Numbers

Deposited strain Deposit number  SJP6840AF4 KCCM-10695P  SJP6844AF5 KCCM-10696P  SJP6726AF6 KCCM-10697P  SJP6843AF7 KCCM-10698P

Odor prevention effect request

6 kinds and 2 for a sample mixed with each culture for 11 kinds of separating cars were to Sejong University, Earth and Environmental Sciences, Kim, Ki - Hyun, Professor, gimyonghwi professor and researcher analyzes commissioned to analyze results, remove the first of the present invention is paper, "Corruption and odor It is published in the September issue of the Journal of Environmental Analysis, entitled “A Study on the Prevention Characteristics and Reduction of Corrupt Odor Using SJP Microorganisms ”.

The test method is as follows: “An analysis of odor changes was conducted for 30 days from the day of inoculation of SJP microorganisms by decaying fish and meat. The results of the analysis show that the SJP microbial treatment results in thousands of times of mitigating effect up to 1 hour and 1 day, and decreases with time, reducing 7 times after 7 days. In particular, hydrogen sulfide in the sulfur series is reduced by 99.99%. Even traces of the odor can not be found, the odor occurs again after 30 days. However, most organic wastes are treated within two to three days, which is effective against odors. ” This refers to the odor prevention effect and anti-corruption effect for 30 days, it can be seen that the SJP microorganism of the present invention can be used as a preservative such as freshness for maintaining fish and the like.

Reverse antibacterial of harmful bacteria

The inventors have confirmed that harmful microorganisms also produce antimicrobial activity in various long-term tests. If the SJP microorganism of the present invention is continuously used for more than three months in a certain device (A: food waste treatment device), it can be seen that the effect of preventing odor and preventing flies gradually decreases. However, if the microorganism is used in the B device in the other place, the effect is the first use of the A device. This can only be understood as evidence of antimicrobial activity or immunity against SJP microorganisms in the microbial world.

This is due to the phenomenon of nature, the ability of the microorganisms to become resistant to a particular microorganism is relatively reduced in capacity. Therefore, more preferably, one or two of the seven yeasts and one or two of the 10 strains of Bacillus strains are cultured and mixed at a constant rate, but at least one month interval is used to exchange the spawns for reverse antibacterial problems. Can be solved. In fact, the above method was applied to food waste processing facilities and containers for storing foreign substances such as plastic bags and meats, which cannot be recycled, and thus prevented odors and flies.

In addition, the antibacterial problem of the harmful bacteria is also applied to the production of livestock feed. If you use only one effect, the antibacterial ability of the enteric harmful E. coli in the livestock is strengthened, so the expected effect is not expected. Therefore, the feed additives as well as the fermented foods described later need a periodic change.

Cattle and pig feed test

Paying attention to the fermentation ability, anti-corruption ability and alcohol fermentation ability, the microbial feed was tested by adding it to Korean cattle, pigs and poultry feed. By adding feed additives or culture liquids to the drinking water, the odors and maggots do not occur in the livestock in any time and anywhere within 7 days of salary. While corn was defecated before use, it was defecated completely after feeding SJP microbial feed additives or microbial culture liquid, and maggots could not be found in the stock.

The SJP microorganisms used in the feed additive preparation used one species of Saccharomyces and Candida 1 and 2 and Bacillus genus among the first and second isolates identified yeast. This is most effectively judged in various tests. The manufacturing method includes the steps of diluting rice bran and bran soybeans at a predetermined ratio, increasing the organic material to 121 ° C. or higher, and immediately inoculating the SJP microorganisms after diluting the three SJP microorganisms at a temperature of 30 to 40 ° C. The feed additive is formed by the step and the step of fermenting it for 30 to 40 hours, the step of drying the fermentation material and the method of grinding the dry matter. More preferably, 5 to 10 kinds of SJP microorganisms are cultured and inoculated by mixing in the inoculation step. In addition, the negative agent to be added to the negative water is mixed with a certain ratio of three or more cultures of Bacillus, including SJP microbial yeast in a proportion of 1 to 2% dilution in the negative water. It is not possible to find the corncress used as cattle feed in both the manure fed the feed additive or the negative preparation prepared as described above. However, in control cows that were not fed a large amount of corn was not digested, it can be confirmed that defecation. This proves the digestion promoting effect and the evidence that it is effective in reducing feed and promoting growth.

Three months of feeding the SJP microbial feed to cattle, pigs and poultry farms resulted in an average 15% feed savings. In addition, if you look at the actual situation of poultry farms in the poultry farms are placed in the process of receiving poises. However, since the chaff is not corrupt, it is not accepted by the composter, so the chicks should be repeatedly used and shipped three or four times. Problems caused by repeated use are a lot of odors and gases are generated, many problems such as disease-induced growth and growth disorders, odors and flies, environmental problems caused by chicken weakness.

Again, spraying the SJP microbial culture solution completely solves the problem.

Livestock Feed Antibiotic Alternative Test

This technology was selected for the 2006 SME Technology Innovation Project supported by the Small and Medium Business Administration, and received 100 million won for antibiotic replacement, growth promotion, feed reduction effect, etc. A consignment test was conducted to verify the odor and flies prevention effects.

Feed additives using SJP microorganisms were effective even with one yeast seed. However, one or two varieties of Bacillus were found to be more effective.The feed additive used in this test was milled and sterilized by 40% of rice bran, 30% of bran, 30% of red pepper seeds, golden, ginger, cinnamon and licorice mixture. Inoculation of SJP6728AF1, SJP6720L3, and SJP6732B2 spawn for 3 minutes, followed by cultivation of SJP microbial feed additive mixed with a certain ratio, 200 pigs in 4 test groups for 4 weeks, and the antibiotic replacement effect was investigated as shown in Table 10.

Table 1 0. SJP microorganism grower antibiotic alternative test

division  Weight gain Feed intake Feed efficiency Remarks Antibiotic Feed Control 840.9 1908.0 2.28 Male and female 20 males 4 repetition examination average Antibiotic Feed Control 695.4 1830.0 2.61 Male and female 20 males 4 repetition examination average Antibiotic feed SJP microbial treatment zone 818.8 1839.1 2.25 Male and female 20 males 4 repetition examination average Data; Cheonan Yonam University Industry-University Cooperation SJP Microbiology Project

Table 10 is the data published in the Cheonan Yonam University paper, and the test results are 2.25. This means that the lowest value is the most effective, which means the increase in the amount of feed intake, and the SJP microbial treatment has the lowest feed efficiency. Therefore, according to Yonam University research team's evaluation, “In the test results, SJP microorganisms fed liquid broilers showed a daily gain similar to that of antibiotics. In particular, SJP microorganisms are more effective as antibiotic substitutes because their feed efficiency is more effective than antibiotic treatment. Was judged to be absent. ”

In addition, the feed additives using SJP microorganisms (one yeast genus, two Bacillus genus) were requested to the Poultry Division of the National Livestock Research Institute of Rural Development Administration. same.

Table 1 1. SJP microorganisms added benefit impact on the broiler productivity

division Antibiotic Antibiotic SJP Additive 05% SJP Additive 1.0% Initiation weight 46.5 ± 0.20 45.7 ± 0.29 46.3 ± 0.27 45.6 ± 0.57 Weight 1,235 ± 80.58 1,479 ± 130.66 1,506 ± 16.01 1,531 ± 19.09 1 day weight gain 34.7 ± 2.14 36.9 ± 2.55 38.2 ± 0.92 38.4 ± 0.22 Daily Feed Intake 64.4 ± 1.20 65.7 ± 1.50 65.1 ± 4.21 68.7 ± 1.79 Feed requirement 1.86 ± 0.09 1.78 ± 0.10 1.70 ± 0.09 1.81 ± 0.02 Source: Rural Development Administration, Livestock Research Institute

The Livestock Research Institute conducted five technologies simultaneously, combining three technologies known to have the best antibiotic replacement effect, the technology developed by the Livestock Research Institute, and the SJP microorganism of the present invention. In this test, SJP microbial agent was evaluated as the best antibiotic replacement effect, feed saving effect and odor prevention effect.

It is proved that the present invention completely solves the problem of human against antibiotics. In particular, in Korea, where livestock farms are small, the amount of antibiotics used is 1,500 tons per year, which is 2 to 10 times higher than that of developed countries. Therefore, it is analyzed that domestic antibiotics play a large part in the Korean population reaching the world's No. 1 antibiotic resistance rate.

In the livestock industry, the next most serious problem is the occurrence of odors and flies. Cheonan Yeonam University analyzes the amount of money generated in the above test and analyzes the effect on the intestine and the degree of odor.

Table 12. Effect of SJP Microbial Addition on Growth and Harmful Gas Production in Growth Pigs

process T1 (antibiotic feed) T2 (Antibiotic Feed) T3 (without antibiotic SJP) LSD (0.05) Microbial count Total bacteria 22.3? 10 ⁶ 35.2-10 ⁶ 33.1? 10 ⁶ NS Escherichia coli 37.2? 10 ⁴ 32.1? 10 ⁴ 9.3-10 ⁴ 22.2 Hazardous Gases (ppm) ammonia 1.4 1.8 0.3 0.8 Hydrogen sulfide 49.0 31.5 0.1 22.0 Source: Cheonan Yeonam University, SJP Microbiology Research Project

Table 12 shows the results of Cheonan Yeonamdae growing pig test results. "We improved our environment and increased our intake."

In addition, the results of harmful gas (odor) analysis concluded that "the results of analysis of harmful gases SJP microbial additives were evaluated to be effective in reducing ammonia and hydrogen sulfide in the pig's harmful gas."

From June 15 to July 15, food wastes were collected at restaurants, etc. in Gulim-dong, Siheung-si, Gyeonggi-do, and sterilized by heating method. At the time of food waste collection, the feed additive was collected by diluting 1 to 2%, and the feed use test was conducted without sterilization treatment. As a result, it prevented the occurrence of odor and flies, and had a strong growth promoting effect without a single death.

Infectious diseases such as bird flu and foot-and-mouth disease in livestock, and mold, pathogenic bacteria in fisheries, and Vibrio bacteria that are fatally damaging to humans are enormous. Prevention of the disease, as well as anti-bacterial garlic and ginger, pepper seeds, licorice, cinnamon as a method of reducing mortality. If one of the seven kinds of SJP microbial yeast is selected and fermented, it is expected to replace the growth-promoting antibiotics and prevent various diseases. Indeed, there are a number of livestock farms that have replaced antibiotics using medicinal herb gold as feed additive. Therefore, fermenting the herbal medicine can increase the efficacy of antibiotics when used as a feed additive.

Herbal Fermentation Feed Additive Test

The present inventors think that SJP microorganism is a microorganism that survived the spraying of natural insecticides made with toxic plants, so that it can solve the toxicity problem in ginkgo biloba leaves and herbal medicines. It became. It was inoculated in 18 plastic bags and inoculated with 17 kinds of SJP microorganisms at 30 ° C each, and put into a warmer at 40 ° C with a non-inoculated control group and confirmed after 24 hours of fermentation. Gas was generated in the inoculation test zone. All seven test strips were considered to have no gas in fermentation for 7 days, and the fermentation was completed.They were put into the mouth to check for detoxification. Received. However, the non-fermented extract vibrated with a tingling sensation, vomiting, aching tongue, and an unpleasant smell.

Ginkgo biloba is known to be used as an insecticide because it has a strong insecticidal effect. Nevertheless, the fact that the microorganisms are not sterilized and fermented is in fact not understandable by the inventors themselves, and in particular, the fact that lacquer is strong because of its antiseptic power is a well-known common sense. But it is true.

Ginkgo biloba leaves contain ginkgolite, flavonoids, teponin, and viramid, which are effective in preventing and treating thrombolytic action, platelet disease, hypertension, diabetes, senile dementia, neurological disease, and cerebrovascular disease. In addition, herbal medicines based on synonym and anti-medical medicine were not used because they could not easily remove toxicity. In the future, it is expected that healed blood vessel patients will be prescribed quickly in blood-related patients. Fermented ginkgo biloba leaves were fermented as a feed or feed additive in pigs, poultry, and cattle, and the ginkgo biloba leaves were fermented as follows.

In mid-August, 17 kinds of ginkgo biloba fermentation compositions were prepared by harvesting ginkgo biloba leaves, drying, pulverizing, sterilizing, 17 isolates, and inoculating fermentation, drying, and milling, respectively. Ginseng, red pepper seeds, licorice, cinnamon by the method described above. 17 kinds of herbal fermentation compositions were prepared by diluting gold in the same ratio. In the ginkgo biloba composition, 50% of the yeast seed fermentation composition and two Bacillus seed fermentation compositions were mixed at the same ratio, and 50% of the herbal fermentation composition was mixed to prepare a herbal feed additive.

Meanwhile, 300 broiler broilers tested at the Rural Livestock Research Institute for 5 weeks were dispersed in 10 cages by 30 water, and the SJP microbial liquid was administered as shown in Table 13, and at the same time, 1% of the herb feed additives were diluted in feed. After broiler testing was conducted from day 05, the average 30-water starting weight and 30-hour average weight gain were checked after 8 days. The control group showed weight gain of around 400g, but the control group showed weight gain of around 600g. The growth effect, odor occurrence and maggot occurrence result of the test are shown in Table 13.

Table 13. SJP Growth and odor of microorganisms and fermented ginkgo biloba leaves, Flies prevention  Test result

  division SJP microbial liquid phase Herbal Additives (%)  Initiation weight Weight Odor 1m rear Maggot outbreak SJP6728AF1 2% 0 1410 g 1.802 g radish radish SJP6729AF2 2% 0 1,415 g 1,797 g radish radish SJP6730AF3 2% 0 1,439 g 1832 g radish radish SJP6740AF4 2% 0 1,418 g 1.840 g radish radish SJP6744AF5 2% 0 1.444 g 1,853 g radish radish SJP6726AF6 2% 0 1,420 g 1,884 g radish radish SJP6743AF7 2% 0 1,489 g 1,801 g radish radish SJP6728AF1 2% One 1,497 g 2.102 g radish radish SJP6730AF3 2% One 1,445 g 2.051 g radish radish SJP6740AF4 2% One 1,433 g 1,984 g radish radish

The present inventors did not confirm the accumulation of ginkgo biloba in the meat of the livestock, but compared with the results of the study that there is ginseng in the meat of cows, pigs, and chickens fed the ginseng leaf, it is expected that the beneficial medicinal ingredients are accumulated in the human body. It can be well received by consumers without regard to the treatment of vascular diseases. The most worrying disease in meat eating is vascular diseases such as blood clots. Therefore, the fact that there is a drug component that has a vascular disease effect on the meat can create added value.

Other Feed Manufacturing Methods

If you look at the actual situation of poultry farms, they are placed in a poultry farm and treated with poultry. However, since the chaff is not corrupt, the composter will not accept it, so the chicks should be used and shipped three or four times. Problems caused by repeated use are a lot of odors and gases are generated, many problems such as disease-induced growth and growth disorders, odors and flies, environmental problems caused by chicken weakness. This problem is solved simply by spraying any one or more of the seven types of yeast of the present invention.

After harvesting, the straw is tied to make straw and used as cattle feed. At this time, the SJP microorganisms are sprayed to make the sieving and then ferment naturally. Therefore, when used as cattle feed, there is no need for a separate microbial feed additive, and it is possible to obtain high-quality fertilizer by harvesting high-speed fermentation of livestock while solving the growth promoting effect and digestive odor flies problem due to the digestion promoting effect. Can be. In addition, when used as an additive in the production of raw grass or hay harvested from grassland, the efficiency of enzyrage can maximize the effect of promoting livestock growth, odor prevention, and flies prevention.

Fermenting plants with a lot of yellow pigments (kisantophyll, carotene), such as corn and gold (medicinal herb), has the effect of increasing the color of yellow. Therefore, when used as a poultry feed is the advantage that the chicken's leather and eggshell and egg yolk becomes a darker yellow. In particular, the fact that fermentation of organic matter further decomposes all the components more finely, the fact that the absorption rate is increased in the human body or the body of the animal is known that the effect can be expected. In fact, the fermentation of gold in the non- steamed state can be seen that the color is changed to more vivid yellow.

Primary organic substance decay test

In order to confirm the anti-corruption effect of 6 primary SJP microorganisms and 11 secondary SJP microorganisms, they were inoculated in water and inoculated with the 17 species, respectively. Observation was made at room temperature in the laboratory. Uninoculated controls began to develop odor after 24 hours. However, all of the SJP microbial treatments did not feel bad smell after 3 days. However, on the 4th day, SJP microorganisms in Bacillus except yeast began to develop fine odors. After 5 days, yeast-treated odors were enough to be felt by people who are sensitive to odors. Occurred to a degree. When the test tofu was removed from the water and the odor and texture of the tofu were examined, the odor occurred on the surface of the tofu, but it was confirmed that the inside of the tofu remained intact and the tissue and firmness of the tofu were the same as the first.

Second anti-corruption test

In order to confirm the anti-corruption effect, 19 test vessels containing three fresh mackerel were prepared, inoculated with 17 kinds of SJP microorganisms, and the salt-sprayed control and the untreated control were each placed in a transparent airtight container at room temperature in summer. The result was examined by the method of leaving and confirming the smell. After 1 day, odors occurred in the untreated control, and microscopic odors occurred in SJP microorganisms of Bacillus after 4 days, and maggots occurred in the untreated control after 7 days. After seven days, all seven SJP microorganisms (seven yeast) treatments began to smell, and maggots also began to develop in SJP microorganisms of Bacillus. However, maggots did not occur in yeast SJP microorganisms even after 15 days. The salted control mackerel was able to feel some odor after 15 days.

In another decay effect test, soybeans were soaked in water for 2 hours, then treated with 17 SJP microorganisms and treated with untreated control, sealed in a plastic bag with a zipper, left in the office at 20-25 ℃, and observed. The control began to mold and smell after 5 days. After 20 days, SJP microorganisms of Bacillus discolored to black, showing rot, but no odor. However, most of SJP microorganisms in yeast were maintained for 42 days with the original form intact, and then the color gradually changed to brown and turned black at 60 days. As a result of releasing the seal and checking whether odor was generated, fermentation was in progress.

As another method, soybeans were soaked in water for 10 minutes to absorb moisture in soybeans, and the feed additive powder prepared by using SJP microorganism spawn in yeast was mixed with 1/10 of soybeans and sealed in the plastic bag. After three months, the original form remained intact. Based on the above test results, it is expected to be used as a preservative for grains, fruits, vegetables and shellfish.

Bean sprout cultivation test

There are many other applications of SJP microorganisms. For example, it can be applied to bean sprout cultivation by using the antiseptic effect. The biggest problem with bean sprouts cultivation is that they often decay even before they grow, so they use chemical preservatives that are harmful to the human body. SJP microorganisms can be sprayed by diluting SJP microorganisms in soybean sprouts or spraying either SJP microbial yeast or Bacillus twice or three times a day to avoid spoilage.

In the bean sprout cultivation test of the present inventors, even though the decay factor was selected by selecting the most decayed summer season, all the test spheres sprayed with SJP microorganisms (17 species) in yeast were confirmed to be helpful for promoting growth. .

SJP Microbial features

As described above, SJP microorganisms include 7 species of yeast isolated from two strains of Saccharomyc and Candlda , and 10 strains identified as Bacillus, including Lactobacillus penivacillus . . As a result of comprehensive analysis of the results of a number of tests of the 17 kinds of SJP microorganisms in various ways as described above, organic fermentation ability, anti-corruption ability, odor prevention ability, insecticidal ability, sterilization ability (five abilities) beyond the imagination It is a common opinion among microbiologists that it is so excellent that it is hardly understood by the known microbiology.

In particular, yeast is easy to cultivate in any anaerobic and aerobic environment and shows an excellent effect compared to Bacillus except for the bactericidal ability of the five abilities in any environment. In many trials, much effort has been made to find differences in the ability of each of the yeasts, but the conclusion is that the superiority crosses depending on which component of the medium was incubated. Thus, it was not possible to specifically mask the superiority.

In addition, analysis of the five abilities of SJP microorganisms (Bacillus), fermentation capacity and insecticidal capacity was less than yeast, but in the bactericidal test, SJP6722A5 of Lactobacillus and SJP6735B6 strain of Paenibacillus , Lactobacillus SJP6841L2 strain, Camo tumefaciens (Camobacterium) in SJP6732B2 strain, Bacillus penny (Paenibacillus) in SJP6719B3 strain Brescia ratio Bacillus (Brevibacillus) in SJP6734B4 strains were analyzed to be the most superior. However, since the test results are cultured in a single medium, the superiority may cross depending on the components of the medium.

SJP Microbial Culture Method

As a culture method of the SJP microbial liquid agent, a culture medium containing a nutrient such as a carbon source, a nitrogen source, vitamins and minerals is used as a culture medium, such as a culture method of a conventional yeast or lactic acid bacteria, Bacillus microorganisms. In the production of culture medium, animals and plants are extracted by increasing the temperature to 121 ℃, inoculated with SJP microorganism spawn at 30 ~ 45 ℃ and incubated for 24 to 62 hours by aerobic or anaerobic fermentation. Stabilizers can be added to maintain the efficacy of the liquid microorganisms for a long time, and when stored in the spawn, containing glycerol components to be stored below freezing or suspended in sterilized 10% skim milk in order to stably preserve the activity of the microorganisms for a long time. Can be stored.

In the culture method of SJP microbial solids, the culture solution is inoculated into organic solids such as sterile sawdust, rice bran, bran, fine flour, corn flour and fermented anaerobicly or aerobicly. At this time, fermentation efficiency is best used at low temperature drying or freeze drying or frozen storage.

When cultured microorganisms are created in an extreme environment, a protective film for surviving itself is maintained to maintain apo status. It is more effective when used as a spawn or as a feed additive.

Induction of mutations in microorganisms and DNA Operation

Mutation induction and DNA manipulation using microbial cell replacement, light (ultraviolet light), temperature change, and ecological environment changes are common techniques. DNA is mutated due to cell binding or mutation induction of the SJP microorganism of the present invention, microorganism consisting of DNA manipulation is within the scope of the present invention.

1 is a report showing the results of the primary analysis of SJP microorganisms of the present invention

Figure 2 is a report showing the secondary analysis results of the SJP microorganism of the present invention

Figure 3 is a copy of the food waste treatment plant odor measurement report using the SJP microorganism of the present invention

Figure 4 is a photograph showing the maggot insecticidal effect of the SJP microorganism of the present invention

Figure 5 is a photograph showing the alcohol fermentation effect of SJP microorganism of the present invention

6 is a local government citation using the SJP microorganism of the present invention

Figure 7 is a copy of the results of use and use effect of the local government using the SJP microorganism of the present invention

Figure 8 is a copy of the letter from the Environmental Sanitation Service who has been released from the stench.

Figure 9 is a photograph showing the SJP microorganism antimicrobial activity of the present invention

Figure 10 is a stock photo feed SJP microbial feed additive of the present invention

Claims (1)

SJP6719B3 strain of the genus Paenibacillus deposited under the accession number Casey CM-10727P (KCCM-10727P).

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