KR100838048B1 - Application method for nutrition of nature plant with isolated phenolic acid from oak wood - Google Patents
Application method for nutrition of nature plant with isolated phenolic acid from oak wood Download PDFInfo
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Abstract
본 발명은 참나무류로부터 단리된 페놀산(phenolic acids)을 농작물의 천연 영양제로 적용하는 방법에 관한 것이다. 참나무로부터 단리되는 페놀산의 제조는 다음의 방법에 의해 제조된다. 소정의 밀폐된 용기 내에 참나무류 목재 칩과 수산기(-OH)를 함유한 추출용매를 포화상태 하에서 전처리시간 30초 이상 12시간 미만, 처리온도 50℃이상 400℃ 미만, 압력 1kgf/cm2이상 100kgf/cm2 미만의 조건에서 전처리된 목재를 회수하고, 상기 전처리된 목재를 120℃에서 15분 이상 60분 미만으로 고온 추출 처리한 다음, 여과 및 분리 정제 처리를 통해 페놀산 추출액을 얻는다. The present invention relates to a method of applying phenolic acids isolated from oaks as a natural nutrient for crops. The production of phenolic acid isolated from oak is produced by the following method. Extraction solvent containing oak wood chips and hydroxyl group (-OH) in a predetermined sealed container is pre-treated at 30 seconds or more for less than 12 hours under saturation, treatment temperature is 50 ℃ or more and less than 400 ℃, pressure 1kg f / cm 2 or more The pretreated wood is recovered under the conditions of less than 100 kg f / cm 2 , and the pretreated wood is subjected to high temperature extraction at 120 ° C. for at least 15 minutes and less than 60 minutes, and then phenolic acid extract is obtained through filtration and separation and purification.
이러한 방법으로 단리된 페놀산 추출액을 농작물의 천연 영양제로 적용하여 농작물의 뿌리발육 및 활착증진, 영양 흡비율 증대, 작물의 스트레스 완화, 생육증진 등의 생리적 기작 향상뿐만 아니라 과실의 당도증가, 생산량 증가, 상품성 향상 등의 용도로 적용한다.The phenolic acid extract isolated in this way is applied as a natural nutrient for crops to increase the sugar content and yield of fruit as well as to improve physiological mechanisms such as root development and growth of crops, increase nutritional absorption rate, stress reduction of crops, and growth. It is applied for the purpose of improving the marketability.
참나무, 페놀산, 농작물, 추출액, 분리정제, 천연 영양제 Oak, phenolic acid, crops, extracts, tablets, natural nutrients
Description
도 1은 본 발명의 실시 예에 따른 페놀산 추출액의 추출물 함량 측정 결과 그래프,1 is a graph showing the extract content of the phenolic acid extract according to an embodiment of the present invention,
도 2는 페놀산 추출액에 함유된 페놀산 함량 성분 측정결과 그래프,2 is a graph of the measurement results of phenolic acid content components contained in phenolic acid extracts,
도 3은 페놀산 추출액 농도에 따른 상추 생장측정 결과 그래프,3 is a graph showing lettuce growth results according to the concentration of phenolic acid extract,
도 4는 페놀산 추출액으로 된 천연식물 영양제 처리한 상추 생육 평가 사진도,Figure 4 is a photograph of lettuce growth evaluation natural plant nutritional treatment of phenolic acid extract,
도 5는 본 발명의 실시 예에 따른 천연식물 영양제 처리로 필드(Field)에서 직접 재배한 시금치 생육 평가 사진도,Figure 5 is a photograph of the evaluation of spinach growth grown directly in the field (Field) with natural plant nutritional treatment according to an embodiment of the present invention,
도 6은 본 발명의 실시 예에 따른 천연식물 영양제 처리로 필드(Field)에서 직접 재배한 참외 생육 평가 사진도,Figure 6 is a photograph of melon growth evaluation directly grown in the field (Field) by natural plant nutritional treatment according to an embodiment of the present invention,
도 7은 필드에서 적용시킨 페놀산 추출액을 농도별로 처리한 호박의 생육 평가 사진도.Figure 7 is a growth evaluation photograph of the pumpkin treated with the phenolic acid extract applied in the field by concentration.
본 발명은 농작물의 생육을 향상시킬 수 있는 영양원을 참나무류로부터 친환경적인 방법으로 페놀산 추출액을 단리하여 천연식물 영양제로 활용하는 방법에 관한 것이다.The present invention relates to a method for isolating phenolic acid extracts from oaks in an environmentally friendly way to improve the growth of crops and utilize them as natural plant nutritional agents.
일반적으로 목재 추출액, 즉 리그노셀룰로오스계 추출물을 제조한 대표적인 목초액 제품들과 최근에는 다양한 식물 재료(한약재료 등)들로부터 추출물을 획득하여 식물생장제로 제조되고 있지만, 참나무류를 친환경적인 고압처리에 의한 추출과 분리 정제 기술로 페놀산 추출액을 효율적으로 단리하여 농작물의 생육 향상을 도모하는 천연식물 영양제의 적용은 시도된 바 없다.Generally, wood extracts, that is, representative wood vinegar products that manufacture lignocellulosic extracts and recently obtained extracts from various plant materials (medicinal herbs, etc.) are manufactured as plant growth agents, but oaks are used for environmentally friendly high pressure treatment. The application of natural plant nutrients to improve the growth of crops by efficiently isolating the phenolic acid extract by extraction and separation and purification technology by means of extraction has not been attempted.
따라서 본 발명의 목적은 참나무류를 친환경적인 고압처리에 의한 추출과 분리 정제 기술로 페놀산 추출액을 효율적으로 단리하도록 하는 페놀산 추출액 제조방법을 제공하는데 있다. Accordingly, an object of the present invention is to provide a method for producing a phenolic acid extract to efficiently isolate the phenolic acid extract by the extraction and separation and purification technology of environmentally friendly high-pressure oak trees.
본 발명의 다른 목적은 참나무류로부터 단리한 페놀산 추출액을 농작물의 생육 향상을 도모할 수 있는 천연식물 영양제로 제조하는 방법을 제공하는 하는데 있다. Another object of the present invention is to provide a method for producing a phenolic acid extract isolated from oaks as a natural plant nutrient for improving the growth of crops.
본 발명의 또 다른 목적은 참나무류로부터 단리한 페놀산 추출액을 이용한 농작물 재배방법을 제공하는데 있다. Still another object of the present invention is to provide a crop cultivation method using a phenolic acid extract isolated from oaks.
상기한 목적을 달성하기 위한 본 발명은, 고압으로 전처리된 목재를 높은 온도에서 목재 추출액을 얻고, 상기 목재 추출액에 함유한 탄수화물의 성분과 페놀산 성분의 함량을 분석하고, 상기 페놀산을 함유한 목재 추출액을 적절히 이용하여 농업용 천연식물 영양제나 친환경 비료와 같은 농업용 자제로 활용할 수 있게 함을 특징으로 한다. The present invention for achieving the above object is to obtain a wood extract at a high temperature for the wood pretreated at high pressure, to analyze the content of carbohydrate and phenolic acid component contained in the wood extract, containing the phenolic acid The wood extract can be properly used to be used as agricultural materials such as natural plant nutrients for agriculture or eco-friendly fertilizers.
이하 본 발명의 바람직한 실시 예들을 첨부한 도면을 참조하여 상세히 설명한다. Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings.
고압으로 처리된 목재 추출액, 즉 리그노셀룰로오스(lignocellulose)는 리그닌과 셀룰로오스의 합성어로서 일반적으로 목재를 달리 표현한 것이며, 상기 리그노셀룰로오스는 목재를 구성하고 있는 화학적 성분을 강조할 때 사용되는 용어이다. The wood extract, lignocellulose, treated at high pressure, is a compound word of lignin and cellulose, which is generally a different expression of wood, and the lignocellulose is a term used when emphasizing the chemical component of wood.
리그노셀룰로오스인 목재를 구성하는 화학적 조성은 생합성된 것이므로 매우 복잡하지만, 유기화합물이기 때문에 그 원소적 조성은 일반 유기화합물과 같이 탄소(carbon), 수소(hydrogen) 및 산소(oxygen)로 구성되어 있으며, 또한 생체대사로 합성된 것이기 때문에 0.1%이하의 질소와 0.3%정도의 무기물을 함유하고 있다. The chemical composition of wood, which is lignocellulosic, is very complex because it is biosynthesized, but because it is an organic compound, its elemental composition is composed of carbon, hydrogen, and oxygen like general organic compounds. In addition, since it is synthesized by living metabolism, it contains less than 0.1% nitrogen and about 0.3% inorganic matter.
본 발명의 실시 예에서는 목재 특히 참나무류로부터 단리하여 탄수화물이 다량 함유한 페놀산 추출액을 얻도록 구현한다. 참나무류에서 단리하여 얻는 본 발명 의 페놀산 추출액은 100% 천연식물 추출액으로서 토양에 유익한 작용을 할 수 있는 원료액이 된다. 목재 페놀산 화합물의 SOD(super oxide dismutase, 활성산소제거)기능은 세포 내에 이물질을 제거하고, 세포의 호흡회로를 정상화시켜 세포를 건강케 하는 유익한 물질을 포함하고 있다. In an embodiment of the present invention is isolated from wood, especially oak trees, is implemented to obtain a phenolic acid extract containing a large amount of carbohydrates. The phenolic acid extract of the present invention, isolated from oaks, is a 100% natural plant extract, which is a raw material that can have a beneficial effect on the soil. The SOD (super oxide dismutase) function of wood phenolic acid compounds contains beneficial substances that remove foreign substances in the cells and normalize the respiratory circuit of the cells to make the cells healthy.
또한 본 발명의 실시 예에서는 상기의 페놀산 추출액의 함량을 조절하여서 식물, 토양은 물론 인체에도 무해하고, 유해한 식물 병원균에 대한 억제 효과도 있도록 하며, 식물 생육 증진에 도움을 주도록 하는 천연식물 영양제가 되게 구현한다. 즉, 본 발명에서 참나무류로부터 단리된 페놀산 추출액은 친환경 농산물 생산을 위한 100% 천연식물 추출액으로 친환경 농법에서 농작물의 생리활성을 도모할 수 있는 보조제로 사용될 수 있고, 이 페놀산 추출액의 지속적인 이용은 화학 비료에 노출된 토양에서 C/N율의 균형을 이루면서 토양의 성능을 충분히 개선시킬 수 있다. 또한, 이 페놀산 추출액에 함유된 저분자 페놀산은 토양 내 유효 미생물의 활성을 도모 할 수 있다. In addition, in the embodiment of the present invention by adjusting the content of the phenolic acid extract is harmless to plants, soil as well as the human body, and also to the inhibitory effect on harmful plant pathogens, and natural plant nutrients to help promote plant growth Implement In other words, the phenolic acid extract isolated from oaks in the present invention is a 100% natural plant extract for the production of eco-friendly agricultural products can be used as an adjuvant to promote the physiological activity of the crop in an environmentally friendly farming method, the continuous use of this phenolic acid extract Silver can significantly improve soil performance while balancing C / N rates in soils exposed to chemical fertilizers. Moreover, the low molecular weight phenolic acid contained in this phenolic acid extract can aim at the activity of the effective microorganisms in soil.
상기와 같은 페놀산 추출액을 제조하기 위한 본 발명의 실시 예에 따른 제조방법을 상세히 설명하면 하기와 같다. Referring to the production method according to an embodiment of the present invention for producing a phenolic acid extract as described above in detail.
먼저 소정의 밀폐된 용기 내에 참나무류 목재 칩과 수산기(-OH)를 함유한 추출용매를 포화상태 하에서 전처리시간 30초 이상 12시간 미만, 처리온도 50℃이상 400℃미만, 압력 1kgf/cm2이상 100kgf/cm2미만의 조건에서 전처리하여 처리된 목재를 회수한다. First, the extraction solvent containing oak wood chips and hydroxyl group (-OH) in a predetermined sealed container is pre-treated at 30 seconds or more for less than 12 hours under saturation, treatment temperature is 50 ° C or more and less than 400 ° C, pressure 1kg f / cm 2 The treated wood is recovered by pretreatment under the condition of less than 100 kg f / cm 2 .
그런 다음에는 상기 전처리된 목재를 120℃에서 15분 이상 60분 미만으로 고온 추출 처리한 다음, 여과 및 분리 정제 처리를 수행함으로써 본 발명의 실시 예에 따른 페놀산 추출액을 제조 완성하게된다. Thereafter, the pretreated wood is subjected to high temperature extraction at 120 ° C. for at least 15 minutes and less than 60 minutes, followed by filtration and separation and purification to prepare a phenolic acid extract according to an embodiment of the present invention.
본 발명의 실시 예에 따라 제조 완성된 페놀산 추출액 내에 존재하는 탄수화물(Sugar composition)의 성분분석 결과는 하기 표 1과 같다. 표 1에서 볼 수 있듯이 본 발명의 페놀산 추출액내에는 다양한 탄수화물이 다량함유되어 있음을 확인할 수 있다. Component analysis results of the carbohydrate (Sugar composition) present in the phenolic acid extract prepared according to an embodiment of the present invention are shown in Table 1 below. As can be seen in Table 1, it can be seen that various carbohydrates are contained in the phenolic acid extract of the present invention.
그리고 상기 전처리된 목재를 120℃에서 반응시간(Reaction time) 15분 이상 60분 미만으로 처리하여서 얻어진 추출물(Extracts)의 함량 결과는 도 1에 도시된 그래프와 같이 170∼172mg g-1범위로 나타나며, 이 조건에서 생산되는 페놀산(Phenolic acid)의 함량은 도 2에 도시된 그래프와 같이 48∼50mg g-1범위를 나타낸다. And the content of the extract (Extracts) obtained by treating the pretreated wood at 120 ℃ reaction time (Reaction time) 15 minutes or less than 60 minutes is shown in the range of 170 ~ 172mg g -1 as shown in the graph shown in FIG. , The content of phenolic acid (Phenolic acid) produced under these conditions is in the range of 48-50mg g -1 as shown in the graph shown in FIG.
본원 발명자는 본 발명의 실시 예에 따른 제조방법으로 제조된 참나무의 페놀산 추출액을 이용하여 실험실 상에서 상추 발아 및 생장시험을 하였다. The inventors conducted lettuce germination and growth test in the laboratory using the phenolic acid extract of oak prepared by the manufacturing method according to the embodiment of the present invention.
본 발명의 실시 예에 따른 방법으로 제조된 페놀산 추출액을 추출원액 100%로 하였으며, 상기 추출원액의 농도를 0.4중량%, 0.2중량%, 0.1중량%로 물에 희석되게 하여서 된 액상의 천연식물 영양제를 상추에 시비한 후 각 상추 종자의 발아율과 상추 생장을 조사하였다. 대조구(Control)는 증류수를 시험구와 동일한 량으로 처리하였다.The phenolic acid extract prepared by the method according to the embodiment of the present invention was 100% extract stock solution, and the concentration of the extract stock solution was 0.4% by weight, 0.2% by weight, 0.1% by weight of the liquid natural plant was diluted in water After nutrient application to lettuce, the germination rate and lettuce growth of each lettuce seed were examined. Control was treated with distilled water in the same amount as the test.
종자의 발아율은 직경 9㎝ 페트리접시(petridish)속에서 필터종이(filter paper)(Whatman No. 2) 1매를 놓고 그 위에 20립의 종자를 흩어 놓은 후, 12시간 마다 각 희석배율액 3㎖를 처리하여 25℃ 암조건에서 5일간 발아시켜 조사하였으며, 각 3회반복의 평균값으로 나타내었다.Seed germination rate was placed in a filter paper (Whatman No. 2) in a 9 cm diameter petri dish, 20 seeds were scattered thereon, and each diluted magnification was 3 ml every 12 hours. Was treated by germination for 5 days at 25 ℃ dark conditions, it was shown as the average value of each of three repetitions.
상추 생장실험은 상추 종자를 원예용 포트(pot)에서 시중에 판매되고 있는 H종묘사의 원예상토를 구입하여 10일후 상추의 길이생장을 측정하였으며 3회 반복 실험하였다. In the lettuce growth experiment, lettuce seeds were purchased at the horticulture pot, and the length of lettuce was measured after 10 days, and repeated three times.
상기의 조건으로 페놀산 추출액으로 된 액상의 천연식물 영양제를 상추의 발아시험에 적용해 본 시험 결과는 하기 표 2와 같으며, 상추의 생장 시험에 대한 결과는 도 3에 도시된 그래프와 같다. 그리고 원예용 포트(pot)에서 페놀산 추출물로 된 액상의 천연식물 영양제를 적용한 처리구와 증류수를 적용시킨 무처리구의 상추의 생장시험 결과 사진은 도 4와 같다.Under the above conditions, the test results of applying the natural plant nutrients of the phenolic acid extract liquid to the germination test of lettuce are shown in Table 2 below, and the results of the growth test of the lettuce are shown in the graph shown in FIG. 3. And the result of the growth test of lettuce in the treatment and the distilled water was applied to the lettuce treated with a natural plant nutrient of the phenolic acid extract in the gardening pot (pot) as shown in FIG.
표 2 및 도 3에 나타난 바와 같이, 시험실 상에서 본 발명의 실시 예에 따른 제품을 무처리구와 대비하여 각 농도별로 생장시험을 한 결과, 페놀산 추출액 0.1∼0.2중량% 농도 처리구는 무처리구에 비해 발아율이 높았으며, 생장 면에서도 향상된 결과를 나타내었다. As shown in Table 2 and Figure 3, as a result of the growth test for each concentration of the product according to the embodiment of the present invention in the laboratory compared to the untreated group, 0.1 to 0.2% by weight of the phenolic acid extract treatment treatment germination rate compared to the untreated group It was high and improved in terms of growth.
따라서 본 발명에서는 참나무류로부터 단리된 페놀산 추출액을 100% 추출원액으로 하고 물에 희석되는 추출원액의 농도 0.1∼0.2중량%가 된 것을 액상의 천연식물 영양제로 활용한다. 즉 본 발명에서는 참나무류로부터 단리된 페놀산 추출액을 100% 추출원액으로 하고 물에 상기 추출원액의 농도 0.1∼0.2중량%가 되도록 희석하여서 액상의 천연식물 영양제로 제조한다. 이러한 본 발명 액상의 천연식물 영양제는 친환경 농업용 자재로서 유용하게 활용될 수 있다. Therefore, in the present invention, a phenolic acid extract isolated from oaks is used as a 100% extraction stock solution, and a concentration of 0.1-0.2% by weight of the extraction stock solution diluted in water is used as a liquid natural plant nutrient. That is, in the present invention, the phenolic acid extract isolated from oaks is prepared as a 100% extraction stock solution and diluted in water so as to have a concentration of 0.1-0.2% by weight of the extraction stock solution to prepare a liquid natural plant nutrient. Such natural plant nutrients of the present invention can be usefully used as environmentally friendly agricultural materials.
본원 발명자는 본 발명의 실시 예에 따른 방법으로 제조된 페놀산 추출액의 필드(field) 적용을 위해, 실제 농가 작물인 시금치, 참외, 호박의 생육에 대하여 평가 시험을 하였다. 평가시험에서는 본 발명에서 제조된 페놀산 추출액을 추출원액 100%로 하였으며, 필드(field) 적용은 페놀산 추출액(천연식물 영양제)과 일정비율 물과 희석하여 엽면살포로 작물의 생육평가를 무처리구와 비교하였다.The inventors of the present invention, the field of the phenolic acid extract prepared by the method according to the embodiment of the present invention, the evaluation test for the growth of the actual farm crops spinach, melon, pumpkin. In the evaluation test, the phenolic acid extract prepared in the present invention was 100% of the extraction stock solution, and the field application was diluted with the phenolic acid extract (natural plant nutritional agent) and a predetermined ratio of water to evaluate the growth of crops with foliar spray. Compared.
먼저, 시금치 하우스 재배에 본 발명의 실시 예에 따른 방법으로 제조된 페놀산 추출액(천연식물 영양제)을 적용해 보았다. 페놀산 추출액과 물의 혼합비율을 500배하여 시금치의 뿌리부분까지 흡수될 수 있도록 엽면 살포 또는 관주 살포하였으며, 그 결과는 도 5의 결과 사진과 같으며, 페놀산 추출액 처리구가 무처리구보다 시금치의 뿌리발육, 생육 속도와 잎의 크기 등이 크게 향상되었음을 확인할 수 있었다. First, phenolic acid extract (natural plant nutrient) prepared by the method according to the embodiment of the present invention was applied to cultivation of spinach house. The foliar spray or irrigation was sprayed to absorb up to 500 times the mixing ratio of phenolic acid extract and water to the root of spinach, and the results are as shown in the photograph of the result of FIG. 5. In addition, growth rate and leaf size were significantly improved.
다음으로, 참외 하우스 재배에 본 발명의 실시 예에 따른 방법으로 제조된 페놀산 추출액(천연식물 영양제)을 적용해 보았다. 페놀산 추출액과 물의 혼합비율을 500배하여 참외의 뿌리부분까지 흡수될 수 있도록 엽면살포 또는 관주살포하였으며, 그 결과는 도 6의 결과 사진과 같으며, 페놀산 추출액 처리구가 무처리구 보다 참외의 생육 속도와 잎의 크기에서 약 2배 정도 향상되었음을 확인할 수 있었다. Next, the phenolic acid extract (natural plant nutrient) prepared by the method according to the embodiment of the present invention was applied to the melon house cultivation. The leaf ratio or irrigation was sprayed so that the mixed ratio of phenolic acid extract and water was absorbed to the root of melon by 500 times, and the result is as shown in the photograph of the result of FIG. 6. It was confirmed that the leaf size was about 2 times improved.
그 다음으로, 호박 하우스 재배에 본 발명의 실시 예에 따른 방법으로 제조된 페놀산 추출액(천연식물 영양제)을 적용해 보았다. 페놀산 추출액과 물의 혼합비율을 100배, 500배, 1000배로 희석하여 호박의 뿌리부분까지 흡수될 수 있도록 엽면살포 또는 관주살포하였으며, 그 결과는 도 7에 도시된 바와 같고 페놀산 추출액 500배 희석한 처리구가 생육이 가장 양호함을 확인할 수 있었다.Next, the phenolic acid extract (natural plant nutrient) prepared by the method according to the embodiment of the present invention was applied to the pumpkin house cultivation. The mixing ratio of phenolic acid extract and water was diluted 100 times, 500 times, and 1000 times to foliar spray or irrigation spray to be absorbed to the root of pumpkin. The result is as shown in FIG. 7 and the phenolic acid extract was diluted 500 times. One treatment was found to have the best growth.
상술한 본 발명의 설명에서는 구체적인 실시 예에 관해 설명하였으나, 여러 가지 변형이 본 발명의 범위에서 벗어나지 않고 실시할 수 있다. 따라서 본 발명의 범위는 설명된 실시 예에 의하여 정할 것이 아니고 특허청구범위와 특허청구범위의 균등한 것에 의해 정해 져야 한다. In the above description of the present invention, specific embodiments have been described, but various modifications may be made without departing from the scope of the present invention. Therefore, the scope of the present invention should not be defined by the described embodiments, but should be determined by the equivalent of claims and claims.
상술한 바와 같이 본 발명은 고압처리된 목재로부터 고온 추출로 획득된 페놀산 추출액을 이용한 친환경적인 천연식물 영양제를 제조하여 농작물에 적용한다면, 농작물의 생리활성을 도모할 수 있을 뿐만 아니라 식물의 발아 및 생장에 매우 효능이 좋은 천연식물 영양제로 비료와 같은 친환경적인 농업용 자재를 제공할 수 있게 되는 효과가 있다. 또한, 본 발명에서 제조된 페놀산 추출액은 탄수화물이 다량 함유된 100% 천연식물 추출액이므로 지속적으로 사용할 경우, C/N율로 인하여 토양의 성능을 충분히 개선시킬 수 있는 잠재력을 가지고 있다.As described above, the present invention, if the environmentally friendly natural plant nutrients using phenolic acid extract obtained by high temperature extraction from the high-pressure wood, and applied to the crops, not only can promote the biological activity of the crops but also germinate and It is a natural plant nutrient that is very effective for growth, and can provide environmentally friendly agricultural materials such as fertilizers. In addition, since the phenolic acid extract prepared in the present invention is a 100% natural plant extract containing a large amount of carbohydrate, it has the potential to sufficiently improve the performance of the soil due to the C / N ratio when used continuously.
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