KR100832242B1 - A patch for extracting an interstitial glucose and an apparatus for measuring an interstitial glucose containing the same - Google Patents

Abstract

The present invention extends from the sensing portion and the sensing portion formed at two or more ion conductive media each containing an enzyme which reacts with glucose to generate a chemical signal, and a position corresponding to the ion conductive medium, respectively. Two extraction electrodes including a connection portion formed to be connected to the electrical measurement unit is formed, the sensing portion formed at one or more positions corresponding to the ion conductive medium and the connection extending from the sensing portion to be connected to the electrical measurement unit A patch for extracting glucose comprising an electrode film having a working electrode, a counter electrode and a reference electrode formed thereon, wherein the extraction electrode, the working electrode, the counter electrode and the reference electrode are coated with an insulating film on the connection part. Characterized in that the patch for extracting glucose and comprising Provides bloodless blood glucose measurement apparatus.

Blood sugar, glucose, no blood

Description

A patch for extracting an interstitial glucose and an apparatus for measuring an interstitial glucose containing the same}

1 is an exploded view of an example of a patch for extracting glucose of the present invention.

2 is a view showing the structure of an electrode film, an ion conductive medium and a carrier film included in an example of the patch for glucose extraction of the present invention.

3 to 6 are views showing examples of the electrode structure included in the glucose extraction patch of the present invention, respectively.

7A, 7B, and 7C are diagrams showing examples of the bloodless blood glucose measurement apparatus of the present invention.

8 is a diagram illustrating a configuration of an example of a blood glucose measurement apparatus of the present invention.

9 is a diagram illustrating an example of an analog switch unit that may be included in the controller.

10A and 10B show blood glucose measurements using a bloodless blood glucose measurement apparatus having an electrode structure 10a and an electrode structure 10b in which an insulating film made of polyethylene acetate is not coated on the connection portion of the electrode portion, respectively. It is a figure which shows.

The present invention relates to a patch for glucose extraction and bloodless blood glucose measurement apparatus comprising the same.

Conventional devices for measuring blood free blood glucose using iontophoresis and glucose extraction patches used therein have been disclosed. For example, Korean Patent Laid-Open Publication No. 1999-0077833 discloses a sampling system for monitoring the concentration of an analyte present in a biological system, comprising: a reservoir containing an enzyme capable of generating hydrogen peroxide by reacting with an ion conductive medium and an analyte; Sampling means for extracting the analyte from the biological system into the reservoir so that the concentration of the analyte in the reservoir that reacts with the enzyme to produce hydrogen peroxide is submillimolar, and is in operative contact with the reservoir; And a sensor element in operative contact with the reservoir, wherein the sensor element is electrochemically reacted with hydrogen peroxide generated in the reservoir to provide a detectable signal, the geometric surface area in the range of about 0.1 to 3 cm ² , measured in buffer at 0.6V. A sampling system comprising a sensor element comprising an electrode having a background current in the range of about 2 to 60 nA or less and a sensitivity in the range of about 6 to 180 nA per μM of hydrogen peroxide when measured in buffer at 0.6 V. It is. In addition, Korean Patent Laid-Open Publication No. 2005-0026791 discloses an ion conductive contact portion in contact with the skin, an electrode portion provided on the opposite side of the surface of the ion conductive contact portion in contact with the skin, and an electric quantity measuring unit electrically connected to the electrode. And a monitor unit connected to the electricity measuring unit, the glucose measuring apparatus comprising: a working electrode to which a voltage for measuring blood glucose is applied; and a glucose surrounding body fluid through the skin and surrounding the working electrode. A first extraction electrode to which a current is applied to extract a second electrode, a counter electrode provided to surround most of the extraction electrode, and electrically connected through the working electrode and the ion conductive contact, and the counter electrode extends. A portion around the extraction electrode and including the working electrode and the reference potential electrode. It is separate from the analysis unit and the analysis unit, and discloses additional electrode of the non-blood glucose measurement device including the extract to a second extraction electrode for applying an extraction current to the skin with the first extraction electrode wherein the analysis portion.

In the case of using a conventional bloodless blood glucose measurement apparatus and a glucose extracting patch used therein, the electrode and the electrode are electrically shorted by a substance (for example, sweat) discharged from the skin. As a result, the signal-to-noise ratio (S / N) of the current measured by the electrode unit may be lowered to reduce the accuracy of the measured value or the electrode unit may be oxidized to make the measurement impossible.

In the conventional bloodless blood glucose measurement apparatus, the ion conductive medium is in contact with the skin when measuring blood glucose, but when the shedding or vibration occurs due to the movement of the wearer of the measuring device, the ion conductive medium is destroyed, or the blood sugar measured thereby There was a disadvantage that the value was incorrect.

However, when using a blood-free blood glucose measurement apparatus and a glucose extracting patch used therein, the electrode and the electrode are short-circuited by a substance derived from the skin (for example, sweat). As a result, the current measured by the electrode is unstable, so that the accuracy of the measurement is inferior.

In addition, in the conventional blood glucose measurement apparatus, the ion conductive medium is in contact with the skin at the time of blood glucose measurement, but is subject to a drooping or twisting force according to the movement of the wearer of the measuring apparatus. In this case, there is a disadvantage that the ion conductive medium is destroyed or the glucose measurement measured therefrom becomes inaccurate. Conventionally, in order to remedy these disadvantages, a device has been developed to protect the ion conductive medium from damage caused by the wearer's movement by using a mold having a hole formed therein to receive the ion conductive medium. However, there is still a need for a method that can solve the disadvantage that the wearer's movement damages the ion-conducting medium or the glucose is inaccurate.

Accordingly, the present inventors have intensively researched to solve these problems, and have now completed the present invention.

An object of the present invention is generated by the electrochemical reaction of glucose in cell hepatitis by coating an insulating film on the connection part of the extraction electrode, working electrode, counter electrode and reference electrode to block the electrical short-circuit by material such as sweat discharged from the skin It is to provide a glucose extraction patch that can measure the exact blood sugar value by increasing the S / N ratio of the amount of electricity.

Another object of the present invention by coating an insulating film on the connection of the extraction electrode, working electrode, counter electrode and reference electrode to block the electrical short circuit caused by the sweat and the like discharged from the skin by the electrochemical reaction of glucose in the intercellular fluid It is to provide a blood-free blood glucose measurement apparatus including a glucose extraction patch that can measure the exact blood sugar value by increasing the S / N ratio of the generated electricity.

The present invention relates to two ionically conductive media in which at least one ionically conductive medium comprises an enzyme that reacts with glucose to generate a chemical signal, and

At least one extraction electrode including a sensing portion formed at a position corresponding to each of the ion conductive media and a connection portion extending from the sensing portion to be connected to an electrical measurement unit, and at least one corresponding to the ion conductive medium A glucose extracting patch comprising an electrode film having a working electrode, a counter electrode, and a reference electrode including a sensing portion formed at a position and a connection portion extending from the sensing portion to be connected to an electrical measurement unit.

The extraction electrode, the working electrode, the counter electrode and the reference electrode is characterized in that the insulating film is coated on the connection portion, provides a patch for extracting glucose.

In the patch for extracting glucose of the present invention, the ion conductive medium may include any medium that can move the ionic material when a current is applied. The ion conductive medium includes a conductive polymer gel and a hydrophilic polymer gel. Preferably, the ion conductive medium is an ion conductive hydrophilic polymer gel. The ion conductive medium may provide a property that can be electrically connected to the extraction electrode for extracting glucose in the cell liver fluid and the detection electrode for detecting the extracted glucose by the surface and reverse iontophoretic contact with the skin, respectively If anything is included.

The enzyme contained in the ion conductive medium includes any one that reacts with glucose to generate a chemical signal, preferably glucose oxidase. The chemical signal may be a substance formed by the enzyme from the glucose or a derivative thereof. For example, the chemical signal may be hydrogen peroxide. The enzyme may be included in either or both of the ion conductive media.

In the patch for extracting glucose of the present invention, the electrode film has two extraction electrodes including a sensing portion formed at a position corresponding to each of the ion conductive medium and a connection portion extending from the sensing portion to be connected to an electrical measurement unit. The working electrode, the counter electrode, and the reference electrode are formed, and each of the sensing portion formed at one or more positions corresponding to the two ion conductive medium and a connecting portion extending from the sensing portion to be connected to the electrical measurement unit Formed. The electrode film may be made of any insulating material known in the art, for example, polyethylene (PE).

When a constant current is applied to the two extraction electrodes connected to the power in opposite directions, the current is applied to the intracellular transdermal fluid through an ion conductive medium in contact with the skin, thereby interacting with cations such as sodium in the intercellular fluid. As a result, cationic glucose accumulates in the ion conductive medium through the skin. The glucose accumulated in the ion conductive medium generates hydrogen peroxide by glucose oxidase immobilized in the ion conductive medium, and the generated hydrogen peroxide is oxidized by a constant voltage applied between the working electrode and the reference electrode, so that Generates electricity

The working electrode, the counter electrode and the reference electrode were extracted from intracellular transdermal fluid by reverse iontophoresis, and the hydrogen peroxide produced by the reaction of glucose accumulated in the ion conductive medium with glucose oxidase immobilized on the ion conductive medium electrochemically. It serves as a detection electrode for detecting an electrical signal generated by oxidation.

The insulating film is made of an insulating material, and after coating, it is impermeable to a material such as a liquid or a gas without generating a change in resistance to the electrode part, that is, the extraction electrode, the working electrode, the counter electrode, and the reference electrode. desirable.

In the patch for extracting glucose of the present invention, the electrode portion, that is, the extraction electrode, the working electrode, the counter electrode and the reference electrode is characterized in that the insulating film is coated on the portion that is not corresponding to the conductive medium, that is, the connecting portion. The material to be impermeable may include water or air. The material of the insulating film may be selected from the group consisting of polyethylene acetate (PEA), polyethylene (PE), polypropylene (PP), polyethylene terephthalate (PET) and silicon oxide, for example, but is not limited thereto. no. The insulating film preferably has the characteristics of an antioxidant film. The process of coating the insulating film on the connection portion of the electrode is known in the art. For example, it is made by screen printing or spray coating, but is not limited to these examples.

The insulating layer may be coated on all or a portion of the connection portion in which the extraction electrode, the working electrode, the counter electrode, and the reference electrode do not contact the conductive medium.

Therefore, in the glucose extracting patch of the present invention, the ends connected to the circuit means (e.g., electricity measurement unit) of the extraction electrode, the working electrode, the counter electrode, and the reference electrode are not coated by the insulating film and are exposed. It may be.

The insulating layer may prevent the detection electrodes from being electrically shorted to each other by moisture such as sweat, thereby distorting the flow of current generated from a chemical signal generated from glucose, and increasing the S / N ratio.

In the patch for extracting glucose of the present invention, materials of the electrode portion, the extraction electrode, the working electrode, the counter electrode and the reference electrode are not particularly limited, but platinum (Pt), gold (Au), silver (Ag) and the like It may be an electrode including carbon (C) or silver / silver chloride (Ag / AgCl) electrode, but is not limited thereto.

Diffusion of glucose extracted from transdermal cell hepatitis using conventional extraction electrodes through the ion conductive medium is perpendicular to the working electrode located at the center of the ion conductive medium and perpendicular to the extraction electrode located at the edge of the ion conductive medium. The diffusion of directions takes place simultaneously. Glucose accumulated at the center of the ion conductive medium is oxidized and exhausted by the working electrode, but glucose extracted by the radial diffusion at the edge of the ion conductive medium is not completely exhausted and a certain amount is continuously accumulated. Alternatively, hydrogen peroxide continuously diffuses into the center portion, which causes an incorrect value of the oxidation current generated by the oxidation reaction.

In the patch for extracting glucose of the present invention, the structure of the electrode is not particularly limited in order to solve the above problems, the electrode arrangement and the reference electrode is positioned at the edge of the ion conductive medium, unlike the existing prior patents, extraction The electrode was designed to be positioned between the working electrode and the counter electrode and the reference electrode. This electrode arrangement is extracted by the radial diffusion generated by the extraction electrode located at the edge of the conventional ion conductive medium because the glucose is oxidized by a constant voltage applied between the working electrode and the reference electrode during the oxidation reaction of the extracted glucose. Glucose is not completely exhausted and a certain amount is continuously accumulated, and thus the glucose or hydrogen peroxide accumulated at the edge is continuously diffused to the center to solve the problem that causes an error in the current.

In the patch for extracting glucose of the present invention, the first extraction electrode of the extraction electrode is located to correspond to one of the ion conductive medium in the form of a ring, the second extraction electrode is the other one of the other ion in the form of a ring cut off The working electrode has a circular shape located inside the annular shape of the second extraction electrode, and the reference electrode and the counter electrode are positioned in line with the cutout portion of the second extraction electrode. It may be.

In the patch for extracting glucose of the present invention, the first extraction electrode of the extraction electrode is located to correspond to one of the ion conductive medium in the form of a ring, the second extraction electrode is the other one of the other ion in the form of a ring cut off The working electrode has a circular shape located inside the annular shape of the second extraction electrode, and the reference electrode and the counter electrode are disposed in parallel to the cutout portion of the second extraction electrode. It may be.

In the patch for extracting glucose of the present invention, the first extraction electrode of the extraction electrode is located in the form of a ring corresponding to one of the ion conductive medium, the second extraction electrode in the form of a ring corresponding to the other one ion conductive medium The working electrode has a circular shape located inside the annular shape of the second extraction electrode, and the reference electrode and the counter electrode are positioned so as to enclose the outer half of the annular shape of the second extraction electrode by about half. It may be.

In the patch for extracting glucose of the present invention, the first extraction electrode of the extraction electrode is located corresponding to one of the ion conductive medium, the second extraction electrode is located corresponding to the other one of the ion conductive medium of the working electrode The counter electrode is provided to surround most of the circumference, and the counter electrode is provided to enclose the majority of the circumference of the second extraction electrode, and the reference electrode is arranged around the second extraction electrode in a portion where the counter electrode does not extend. It may be provided.

Here, the working electrode, the first and second extraction electrode, the counter electrode and the reference electrode may each have a connecting portion extending outwardly in the form of a ring. In addition, the working electrode may be substantially circular, and the area ratio of the working electrode and the counter electrode may be 0.5 times to 1.1.

The electrode structure is 30 to 52 mA per μM of glucose when the base current is 0.5 to 10.0 nA or less and is measured at 0.4 V at 0.4 V in an electrode structure having a geometrical area ratio of 0.5 to 1.1 between the working electrode and the counter electrode. It can have a current amount of.

This is due to oxidation resulting from electroactive species (eg, ascorbic acid, uric acid, etc.) in the intercellular fluid extracted by reverse iontophoresis using a potential lower than the oxidation potential used in Korean Patent Laid-Open Publication No. 1999-0077833. By excluding the current, not only can the glucose selectivity be increased, but the density of the current applied to the transdermal can be reduced, thereby minimizing the inconvenience caused by the electrical stimulation for the user.

In the patch for extracting glucose of the present invention, the electrode film may be a double-sided adhesive tape is attached to a portion except for the sensing portion of the electrode portion corresponding to the ion conductive medium. The adhesive tape serves to fix the patch including the electrode film on the skin of the wearer in use. In this case, therefore, even if there is no frame for immobilizing the ion conductive medium, the ion conductive medium can be stably fixed to the skin to protect the ion conductive medium from the movement of the wearer.

In the patch of the present invention, the ion conductive medium may be accommodated in the two medium receiving portion of the carrier film on the opposite side of the surface in contact with the electrode is formed with two medium receiving portion. The ion conductive medium is accommodated in the carrier film, thereby minimizing damage due to external force and change in moisture content. The carrier film is generally removed after the ion conductive medium is adhered to the electrode of the electrode film in use, but in some cases it may be used as a framework for fixing the ion conductive medium without being removed.

Thus, a first embodiment of the present invention provides two ionically conductive media comprising an enzyme in one or more ionically conductive media that reacts with glucose to generate a chemical signal,

A carrier film having two medium receiving portions containing the ion conductive medium formed therein, and

At least one extraction electrode including a sensing portion formed at a position corresponding to each of the ion conductive media and a connection portion extending from the sensing portion to be connected to an electrical measurement unit, and at least one corresponding to the ion conductive medium A glucose extracting patch comprising an electrode film having a working electrode, a counter electrode, and a reference electrode including a sensing portion formed at a position and a connection portion extending from the sensing portion to be connected to an electrical measurement unit.

The extraction electrode, the working electrode, the counter electrode and the reference electrode is a patch for extracting glucose, characterized in that the insulating film is coated on the connection portion.

In a first embodiment of the patch for extracting glucose of the present invention, the carrier film is provided with a medium receiving portion in which the ion conductive medium is accommodated. The medium receiving portion may be any type known in the art, for example, a groove, a chamber, and a hole (through hole) as long as the medium receiving portion can accommodate the ion conductive medium. Preferably, it has a shape corresponding to the shape of the ion conductive medium and can be accommodated therein. The carrier film may optionally be used as a framework for immobilizing the ion conductive medium without being removed at the time of use. In this case, the receiving portion is preferably in the form of a through hole.

According to a second embodiment of the patch for extracting glucose of the present invention, in the first embodiment, the electrode film has a double-sided adhesive tape on each other except a contact portion between the ion conductive medium and the sensing portion of the electrode portion. It may be bonded.

In the second embodiment of the glucose extracting patch of the present invention, a release film is interposed between the carrier film and the electrode film, and the ion conductive medium is not used. The electrode and the electrode may be in contact with each other, but may be in contact with each other during use. The release film has little or no property of adhering to the adhesive material positioned between the carrier film and the electrode film, and can be easily removed.

Accordingly, in the third embodiment of the patch for extracting glucose of the present invention, in the second embodiment of the patch for extracting glucose of the present invention, the electrode film has two-sided adhesive portions other than the portions corresponding to the two ion conductive media. Coated by tape,

A release film is mediated between the electrode film coated with the adhesive tape and the carrier film in which the two ion conductive media are accommodated in the two medium receiving portions, thereby removing the release film in use, thereby removing the ion conductive medium. And the electrodes of the electrode film are to be in contact with each other.

The present invention also, the glucose extraction patch according to the present invention as described above, the electricity amount measuring unit electrically connected to the electrode portion of the glucose extraction patch, the monitor unit connected to the electricity amount measuring unit, the patch unit It provides a blood-free blood glucose measurement device, including a control unit for controlling the electrical measurement unit, and the monitor unit, respectively.

In the bloodless blood glucose measurement apparatus of the present invention, by connecting the power in the opposite direction to the two extraction electrodes of the glucose extraction patch to extract glucose from the intradermal intercellular fluid with the ion conductive medium, the extracted glucose is The enzyme is converted into a chemical signal in an ion conductive medium. The chemical signal generated in the ion conductive medium is converted into an electrical signal, i.e., a current, thereby generating a flow of current through the detection electrode, and the flow of the current is measured by the electricity measuring unit. The electric charge measuring unit includes any electric charge measuring device known in the art. For example, it can be a measuring device such as current, voltage and resistance. The electricity amount measuring unit may include a power supply unit.

The monitor unit is connected to the electricity amount measurement unit, and displayed in terms of the measured electricity amount itself or the amount of glucose in the blood glucose. The monitor can be any monitor device known in the art, such as CRT, LED, LCD, and PDP. The display method may include digital or analog. The control unit may include a key input unit and a sound output unit.

In the bloodless blood glucose measurement apparatus of the present invention, the monitor may be displayed together with the measurement time and the blood sugar value. In addition, when the blood glucose value is lower than the hypoglycemic reference value (for example, 50mg / dL or less) or higher than the hyperglycemic reference value (for example, 351mg / dL), it may be that the alarm sounds at the same time as the display change of the monitor. For example, if the blood glucose value is lower than the hypoglycemic reference value (e.g. below 50 mg / dL) or the screen display (e.g. LED color) is changed to red, and is higher than the high blood glucose reference value (e.g. 351 mg / dL), it is changed to blue At the same time, a beep may sound. At this time, the low blood sugar or high blood sugar value may be that the measurement time is automatically stored in the memory.

The control unit may include a blood glucose measurement mode and a mode for inputting a correction value.

In addition, the monitor unit may be to display a "START" message when the glucose extraction patch is mounted on the blood glucose measurement apparatus of the present invention. Whether or not the glucose extracting patch is mounted on the bloodless blood glucose measurement apparatus of the present invention may be recognized by a detection apparatus when an cognitive label (eg, a conductive material) is formed on the patch and the cognitive label is introduced into the device. It may be to display on the monitor unit. If the patch is not mounted on the blood sugar measuring device or the blood sugar measuring device is incorrectly worn, the error display may be displayed on the screen display of the monitor.

The monitor unit may include a memory unit capable of storing the measured blood glucose value and a time at which the measured blood glucose value is measured.

In addition, the controller may be a built-in search program for loading or searching the stored memory. Such a search can be any algorithm known in the art and can be searched according to the size of the blood glucose value or by the date of the stored data.

The controller may include an A / D converter and a switch.

In the bloodless blood glucose measurement apparatus of the present invention, the glucose extraction patch is used for reverse iontophoresis to extract the analyte from the biological system.

Blood glucose measurement apparatus of the present invention, every 20 minutes by analyzing the glucose in the intercellular fluid extracted from the percutaneous by reverse iontophoresis and converts the blood glucose value and the result of the bar at the bottom of the blood glucose value at the top of the monitor unit It is graphed. Compared with the conventional bloodless blood glucose measuring device, which merely reads the past blood sugar value, the bloodless blood glucose measuring device of the present invention displays the blood sugar value in the upper part of the monitor when the past blood sugar measuring value is loaded, and the bar graph at the bottom Since the pattern of blood glucose change during each hour before and after is displayed at the same time based on the past point of time, it is possible to predict the pattern of blood glucose change of the current user, thereby coping with hypoglycemia or hyperglycemia. For example, low blood sugar can be used to increase the blood sugar by ingesting high-glucose beverages or candy, or to prevent insulin in case of high blood sugar, thereby preventing shock caused by low or high blood sugar. Make sure

Hereinafter, the present invention will be described with reference to the drawings.

1 is an exploded view of an example of a patch for extracting glucose of the present invention. As shown in FIG. 1, the glucose extracting patch of the present invention includes two ion conductive media 20, a carrier film 30 in which the ion conductive medium 20 is accommodated, and the carrier of the ion conductive medium 20. A first extraction electrode including a sensing portion 16 formed at a position corresponding to the surface opposite to the surface accommodated in the film 30 and a connecting portion 17 extending from the sensing portion to be connected to the electricity quantity measuring unit ( 11) a sensing portion 16 formed at a position corresponding to the surface opposite to the surface accommodated in the carrier film 30 of the other ion conductive medium 20 and extending from the sensing portion so as to be connected to the electricity measuring unit. The electrode film 10 includes the second extraction electrode 12, the working electrode 13, the counter electrode 14, and the reference electrode 15 including the connection part 17 formed thereon. All or part of the connecting portion of the electrode portion of the electrode film 10 may be coated with an insulating film 18. The connecting portion 17 is connected with circuit means (not shown) through an end portion. The circuit means includes an electric quantity measuring unit.

In the patch for extracting glucose of the present invention, the electrode film 10 is an adhesive material, eg, at a portion except for the contact portion between the ion conductive medium (eg, hydrogel) 20 and the sensing portion 16 of the electrode portion. For example, the double-sided adhesive tape 40 may be bonded.

In addition, the patch for extracting glucose of the present invention is an adhesive material, for example, on a portion except for the carrier film 30 and the sensing portion 16 of the electrode portion, in which the two ion conductive media 20 are accommodated in the medium receiving portion. The release film 50 may be mediated between the electrode films 10 to which the double-sided adhesive tape 40 is adhered. The release film is removed during use to bring the ion conductive medium 20 and the electrodes of the electrode film 10 into contact so that the glucose extraction patch can be safely and stably maintained during storage and transportation.

The patch for extracting glucose of the present invention may further include a patch guide 60 for accommodating the patch.

2 is a view showing the structure of an electrode film, an ion conductive medium and a carrier film included in an example of the patch for glucose extraction of the present invention. As shown in FIG. 2, the ion conductive media 220a and 220b have a disk shape of which the area of the lower surface is smaller than that of the upper surface, and the two medium receiving portions of the carrier film 230 are in the form of holes. The hole has a shape corresponding to that of the ion conductive medium. In addition, the electrode film 210 is adhered to the carrier film 230 in which the ion conductive mediums 220a and 220b are accommodated, and a circuit means, for example, is provided through a terminal 211 formed on the back surface of the electrode film 210. It is connected to the measuring device. The carrier film shown in FIG. 2 may be used as a framework for immobilizing the ion conductive medium more stably without being removed in use.

3 to 6 are views showing examples of the electrode structure included in the glucose extraction patch of the present invention, respectively. As shown in FIG. 3, the first extraction electrode 215a of the extraction electrodes is positioned to correspond to one of the ion conductive media in the form of a ring, and the second extraction electrode 215b is formed in the form of a ring in which a portion thereof is cut out. The working electrode 215c has a shape of a circle located inside the annular shape of the second extraction electrode, and the reference electrode 215d and the counter electrode 215e are positioned corresponding to the ion conductive medium. Can be placed in line with the cutout.

As shown in FIG. 4, the first extraction electrode 215a of the extraction electrodes is positioned to correspond to one of the ion conductive media in the form of a ring, and the second extraction electrode 215b is formed in the form of a ring in which a portion thereof is cut out. The working electrode 215c is located in correspondence with the ion conductive medium, and the working electrode 215c has the shape of a circle located inside the annular shape of the second extraction electrode 215b, and the reference electrode 215d and the counter electrode 215e are The cutout portion of the second extraction electrode 215b may be positioned in parallel.

As shown in FIG. 5, the first extraction electrode 215a of the extraction electrodes is located in the form of a ring to correspond to one of the ion conductive media, and the second extraction electrode 215b is the other ion conducting medium in the form of a ring. The working electrode 215c has a shape of a circle located inside the annular shape of the second extraction electrode 215b, and the reference electrode 215d and the counter electrode 215e have the second extraction electrode ( 215b) may be positioned so as to surround each of the outer half of the ring shape.

As shown in FIG. 6, a first extraction electrode 215a of the extraction electrodes is located in correspondence with one of the ion conductive media, and a second extraction electrode 215b is located in correspondence with the other ion conductive medium in the working A counter electrode 215d is provided to surround most of the periphery of the electrode 215c, and a counter electrode 215d is provided to surround most of the periphery of the second extraction electrode 215b. It may be provided around the second extraction electrode (215b) in a portion that does not extend. Here, each electrode may have a connecting portion extending to connect to the terminal.

The patch for extracting glucose of the present invention is used by being attached to a bloodless blood glucose measurement apparatus. The bloodless blood glucose measurement apparatus may take the form of a wrist watch as shown in FIG. 7. On the front of the device, a display such as an LCD for displaying a glucose value measured as shown in FIG. 7A and a button for operating a device of a user are arranged. On the back of the device is attached a patch 200 for glucose extraction, as shown in FIGS. 7B and 7C. To this end, a device may be provided with a locker. In addition, a space for accommodating the battery may be provided. 7b and 7c, the glucose extraction patch and the device may be electrically connected at terminals, respectively.

When the user mounts the device, the surface of the ion conductive medium in the patch contacts the wearer's skin, and when a current is applied to the electrode contacted with the ion conductive medium, reverse iontophoresis leads to the ion conductive medium from the intradermal intracellular fluid. Glucose is extracted. The extracted glucose reacts with the enzymes contained in the ion conductive medium. As a result, current is generated. The device monitors the blood glucose level by measuring the current generated.

Patch 200 may be provided with a terminal for electrical connection with the device. When the patch 200 is attached to the device, the terminal is in electrical connection with the terminal provided on the back of the device. The device applies a current through these terminals and measures the current according to the electrochemical reaction.

8 is a diagram illustrating a configuration of an example of a blood glucose measurement apparatus of the present invention. As shown in FIG. 8, the measuring apparatus of this invention can be comprised from a patch part, an electric quantity measuring part, a monitor part, and the control part which controls these. The patch unit extracts glucose, generates chemical signals, and converts the chemical signals into electrical signals. The electric quantity measuring part is connected to the patch part and functions as a signal detecting part detecting the electric signal. The monitor unit may be connected to the electricity measuring unit and include a display unit such as an LCD and an LED. The controller is connected to the patch unit, the electricity measurement unit and the monitor unit, and may include a switch unit, a current voltage converter, an operational amplifier circuit, an A / D converter, a sound output unit, and a key input unit.

9 is a diagram illustrating an example of an analog switch unit that may be included in the controller. The switch unit controls the extraction current and the analysis voltage by an analog switch according to the control signal of the controller to apply to the extraction electrode, the reference electrode, the working electrode, the auxiliary electrode. The analog switch may be transferred to lead terminals of each of the extraction electrode, the reference electrode, the working electrode, and the auxiliary electrode which are in contact with the ion conductive medium. Accordingly, the analog switch unit is used for interrupting the current path of the circuit as a switch that is switched and controlled by the control of the control unit.

The operational amplifier AMP, which may be included in the control unit, detects the amount of current flowing through the working electrode according to the extraction current and the analysis voltage applied by the control unit, and outputs the voltage.

The A / D converter, which may be included in the controller, converts the analog voltage output from the analog switch unit and the voltage supply source into a digital signal.

The controller controls the operation of the blood glucose measurement apparatus of the present invention. For example, the controller controls the analog switch to supply a current to the extraction electrode to extract glucose from the intradermal intercellular fluid into the ion conductive medium of the glucose extraction patch, and electrochemically oxidize the extracted glucose. In order to supply an applied voltage to the electrode. The controller may include a memory storing a control program that calculates a glucose concentration value corresponding to a voltage value through an internal operation, such as noise removal and error occurrence of the detected signal.

The LCD display unit, which may be included in the monitor unit of the present invention, displays the glucose concentration value measured according to the control signal of the controller.

Example

An insulating film made of polyethylene acetate was coated on the connection portion of the electrode, and blood glucose was measured using a bloodless blood glucose measurement apparatus having an electrode structure as shown in FIG. 1. As a control, blood glucose was measured using a bloodless blood glucose measurement apparatus having an electrode structure as shown in FIG. 1 except that an insulating film made of polyethylene acetate was not coated on the connection portion of the electrode portion.

10A and 10B show blood glucose measurements using a bloodless blood glucose measurement apparatus having an electrode structure 10a and an electrode structure 10b in which an insulating film made of polyethylene acetate is not coated on the connection portion of the electrode portion, respectively. It is a figure which shows. As shown in Figure 10b, the glucose extracting patch of the present invention and the device comprising the same, the S / N ratio is significantly higher than the control.

According to the patch for extracting glucose of the present invention, by coating an insulating film on the connection portion of the extraction electrode, working electrode, counter electrode and reference electrode to prevent the electrical short circuit caused by the material such as sweat discharged from the skin electricity of glucose in the intercellular fluid Accurate blood glucose levels can be measured by increasing the S / N ratio of the amount of electricity generated by a chemical reaction. In addition, in the case where the electrode film is coated with an adhesive material except for a portion corresponding to the ion conductive medium, the ion conductive medium may be stably fixed even if there is no frame for fixing the ion conductive medium.

According to the bloodless blood glucose measurement apparatus of the present invention, the blood sugar can be measured stably without a short circuit, and the blood sugar can be measured at a high S / N ratio. .

Claims (17)

Two ionically conductive media containing enzymes in one or more ionically conductive media that react with glucose to generate chemical signals, and At least one extraction electrode including a sensing portion formed at a position corresponding to each of the ion conductive media and a connection portion extending from the sensing portion to be connected to an electrical measurement unit, and at least one corresponding to the ion conductive medium A glucose extracting patch comprising an electrode film having a working electrode, a counter electrode, and a reference electrode including a sensing portion formed at a position and a connection portion extending from the sensing portion to be connected to an electrical measurement unit. The extraction electrode, the working electrode, the counter electrode and the reference electrode is characterized in that the insulating film is coated on the connection portion, A first extraction electrode of the extraction electrode is located to correspond to one of the ion conductive medium, the second extraction electrode is located to correspond to the other ion conductive medium is provided to surround most of the circumference of the working electrode, The counter electrode is provided so as to surround most of the circumference of the second extraction electrode, the reference electrode is provided around the second extraction electrode in the portion where the counter electrode does not extend, glucose extraction patch. The patch for extracting glucose according to claim 1, wherein the insulating film is coated on all or a part of a connection portion which does not contact the ion conductive medium of the extraction electrode, the working electrode, the counter electrode and the reference electrode. The method according to claim 1, wherein the ion conductive medium, the opposite side of the contact surface with the electrode is accommodated in each of the two medium receiving portion of the carrier film formed with two medium receiving portion, glucose extraction, Dragon patch. The patch for extracting glucose according to any one of claims 1 to 3, wherein the insulating film is selected from the group consisting of polyethylene acetate, polyethylene, polypropylene, polyethylene terephthalate and silicon oxide. The electrode film of claim 1, wherein all or part of the electrode film is coated with a double-sided adhesive tape except for a portion corresponding to the ion conductive medium. A release film is mediated between the electrode film coated with the adhesive tape and the two ion conductive media, so that the electrode portion of the electrode film and the ion conductive medium can contact each other by removing the release film during use. That is, a patch for glucose extraction. The patch for extracting glucose according to any one of claims 1 to 3, wherein the ion conductive medium is an ion conductive hydrophilic polymer gel and the enzyme is glucose oxidase. delete delete delete delete delete delete delete delete delete delete delete

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