KR100815469B1 - Extractor for meiofaunal organisms in sediments using ultra fine bubble generator - Google Patents

Abstract

An apparatus for extracting microbes from bottom materials is provided to increase the recovery rate of the microbes from a suspension of the bottom material and a solution by having a circulation propeller forming vortex in a horizontal direction, observe a clean state microbes by increasing the amount of the microbes moved to a sample collection portion through increasing the floating capability of the microbes, analyze the microbes quantitatively and qualitatively by controlling an extraction time and an extraction amount using a seawater injector and maintain the freshness of a sample and save the time by being portably prepared. An apparatus for extracting microbes from bottom materials comprises: a first circulation portion(10) which mixes a solution injected by a solution injection portion(20) and the bottom materials to generate a suspension and raises the suspension; a second circulation portion(50) which is located at the upper part of the first circulation portion and makes the raised suspension floated using microbubbles having a size of less than 1 mm generated by a microbubble generator(40); and a sample collection portion which is located at the upper part of the second circulation portion and collects the microbes floated by the second circulation portion, wherein the second circulation portion includes an inner pipe(53) where the suspension is bound to the microbubbles, a second outer pipe(55) where the suspension bound to the microbubbles move along the upper side and the outer side of the inner pipe by raising the microbubbles, and a circulation space portion(51) which is coupled to the inner pipe to move the suspension of the first circulation portion and connected to the lower side of the second outer pipe to re-mix the suspension to the first circulation portion. Further, a salt concentration of solution is increased by adding the salt into the solution injected through the solution injection portion.

Description

Low quality microbial extracting device using ultra-mini-foam generator {EXTRACTOR FOR MEIOFAUNAL ORGANISMS IN SEDIMENTS USING ULTRA FINE BUBBLE GENERATOR}

1 is a block diagram schematically showing an apparatus for extracting low quality organisms using an ultra-miniature gun generator according to the present invention.

Figure 2 is a perspective view schematically showing a low quality organisms extraction device using an ultra-miniature gun generator according to the present invention.

Figure 3 is a schematic diagram of the solution circulation of the microbial extracting apparatus using the ultra-miniature foam generator according to the present invention.

Figure 4 is a flow chart schematically showing an extraction method of a low quality organisms extraction device using an ultra-miniature gun generator according to the present invention.

Figure 5 is a perspective view schematically showing a primary circulation of the microbial extraction apparatus using the ultra-miniature gun generator according to the present invention.

Figure 6 is a perspective view schematically showing a solution injecting part of the extraction agent of the low quality organisms using the ultra-miniature foam generator according to the present invention.

Figure 7 is a schematic exploded perspective view and cross-sectional view of the secondary circulation of the microbial extracting apparatus using the ultra-miniature gun generator according to the present invention.

Figure 8 is a perspective view of the air pump and the ultra-mini-foam generator of the microbial extraction apparatus using the ultra-mini-foam generator according to the present invention.

Figure 9 is an exploded perspective view, cross-sectional view and front view of the sample collection unit of the microbial extraction apparatus using the ultra-miniature foam generator according to the present invention.

10 is a view comparing the operation of the ultra-miniature gun generator and the existing generator in fresh water and sea water according to the present invention.

Figure 11 is a view showing the residual amount of time for a low-quality organisms extraction device using the ultra-miniature gun generator according to the present invention.

       <Brief description of reference numerals for the main parts of the drawings>

1: Low microbial extraction device using ultra-miniature gun generator

10: 1st circulation part 11: circulation propeller switch

13: circulation propeller 15: first outer tube

20: solution injection unit 21: tube for solution injection

30: air pump 31: tube for air injection

40: ultra-miniature gun generator 50: secondary circulation

51: circulating space 53: inner tube

55: second outer tube 70: sample injection unit

The present invention relates to a microorganism extraction device using an ultra-miniature artillery generator, and more particularly, by including a circulating propeller for forming a vortex in the transverse direction, to increase the recovery rate of microorganisms from the suspension mixed with lipid and solution. By forming a rising flow using a wood stone or limewood air stone to form an ultra-small force of less than 1 mm, the flotation of the microorganisms is increased, thereby increasing the amount of microorganisms that are transferred to the sample collection unit. As the residual amount is reduced, microorganisms in a clean state can be observed, and the extraction time and extraction amount can be controlled by using a seawater injector, so that qualitative and quantitative analysis of low quality organisms is possible, and can be manufactured by hand. Addition of low quality organisms using ultra-miniature gun generator to maintain freshness and save time It is about an exit device.

In general, a method of extracting microorganisms inhabiting the low quality forms air bubbles of 1 mm or more using ceramic air stones, and supplying them to water may expose microorganisms from the low quality.

And, the floating (Planktonic) microorganisms are moved to the upper surface due to the air bubbles, the moved microorganisms are made to be extracted.

However, the ceramic air stone has a low floatation force of microorganisms due to air bubbles of 1 mm or more, so that the recovery rate is reduced, and the residual amount increases as the suspended particles are also included in the low quality of the suspended microorganisms. It was not easy to analyze, and there were problems such as a decrease in recovery rate due to the formation of sediment in the area where no vortex was formed.

The present invention has been made in order to solve the above problems, by providing a circulating propeller to form a vortex in the transverse direction, to increase the recovery rate of microorganisms from the suspension mixed with lipid and solution, ultra-fine By forming a rising flow using a wood stone or limewood air stone to form a swell, the flotation of the microorganisms is increased, thereby rapidly increasing the amount of microorganisms that are transferred to the sample collection unit, and the residual amount is reduced to a clean state. Microorganisms can be observed, and extraction time and amount can be controlled by using seawater injector for qualitative and quantitative analysis of low quality organisms. The purpose of the present invention is to provide a microorganism extraction device using ultra-miniature gun generator. Shall be.

In order to achieve the object as described above, the present invention includes a primary circulation unit for producing a suspension using a circulation propeller for mixing a solution having a certain salinity with a low quality in the first outer tube of the cylindrical; A solution injecting unit for injecting a solution into the first outer tube using a solution injecting tube; A secondary circulation portion for introducing the suspension liquid of the primary circulation portion with the coercive force generated by the operation of the ultra-fine strength foam generator to generate the ultra-fine foam gun; An air pump for injecting air by using an air injection tube in the secondary circulation part; A sample collecting unit which collects bubbles containing poor quality generated from the secondary circulation by increasing the amount of the injection solution to generate a bubble for a predetermined time, and then reducing the flow rate of the solution injection; It includes.

The secondary circulation portion includes an inner tube in which the suspension liquid of the primary circulation portion is combined with the ultra-mini strength cloth; A second outer tube in which the suspension liquid bound in the inner tube moves along the upper and outer surfaces of the inner tube as the bubbles rise; A circulation space portion coupled to the inner tube to move the suspension liquid of the primary circulation portion, and to remix the suspension liquid to the primary circulation portion by being connected to a lower surface of the second outer tube; Characterized in that comprises a.

In addition, the ultra-mini strength gun generator is characterized in that for generating bubbles of less than 1mm by using a wood stone.

Here, the sample collection unit is coupled to the upper surface of the secondary circulation portion is characterized in that the extraction amount and the extraction time can be adjusted according to the solution injection amount and flow rate.

In addition, in order to increase the biorecovery rate in the reservoir, the circulation propeller is characterized by continuously mixing the reservoir and the solution.

And, in order to increase the biological recovery rate, it is characterized by increasing the concentration of the solution.

Here, the primary circulation portion, the secondary circulation portion, the sample collection portion, the solution injection portion and the air pump is characterized in that the removable and movable.

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings.

1 is a block diagram schematically showing an apparatus for extracting low quality organisms using an ultra-miniature cloth generator according to the present invention, and FIG. 2 is a perspective view schematically showing a mechanism for extracting low-quality organisms using an ultra-miniature cloth generator according to the present invention. to be. As shown in the drawings, the microbial extracting apparatus 1 using the ultra-miniature bubble generator according to the present invention includes a primary circulation part 10, a solution injecting part 20, an air pump 30, and an ultra-fine particle bubble. It comprises a generator 40, the secondary circulation unit 50 and the sample collection unit 60.

Here, the first circulation part 10 is composed of a circulation propeller switch 11, a circulation propeller 13, and a first outer tube 15. The primary circulation part 10 is formed in a cylindrical shape to form the primary circulation. A circulation propeller switch 11, which is a switch for driving the circulation propeller 13, is provided on a lower surface of the part 10, and the circulation propeller 13 driven according to an on / off signal of the circulation propeller switch 11. ) Is provided to be electrically connected to the upper surface of the circulation propeller switch (11).

Then, the vortex in the transverse direction so that the low-quality material including the solution injected into the primary circulation portion 10 and the mud containing the Planktonic microorganisms on the upper surface of the circulation propeller 11 is mixed. This is because, due to the mixing action in the first outer tube 15, the bottom quality and the solution is formed into a suspension, and the microorganisms contained in the bottom quality are made to mix well with the solution without sinking.

In addition, the solution injection unit 20 injects the solution through the solution injection tube 21 into the first outer tube 15 of the primary circulation unit 10, the solution is usually used to Increasing the concentration of the seawater may increase the recovery rate, which is the extraction amount of the microorganisms contained in the reservoir, and when the salt concentration of the solution is increased, the recovery rate of the floating organisms is increased, thereby enabling quantitative analysis.

In addition, the air pump 30 is transferred to the ultra-miniature bubble generator 40 through the air injection tube 31, the ultra-miniature bubble generator 40 is an ultra-fine bubble generator that generates bubbles of 1mm or less When the ultra-miniature gun generator 40 located in the cylindrical inner tube 53 of the secondary circulation unit 50 is operated, the ultra-mini-foam guns are raised to the upper surface through the inner tube 53. Decompression occurs in the lower surface of the inner tube (54), so that the inner tube (53) and the first outer tube (15) penetrates to connect, so that the suspension liquid forming a vortex in the transverse direction in the first outer tube is the inner Flows into the tube (53).

Here, the ultra-mini-foam generator 40 is characterized in that the generation of the ultra-mini-foam gun of less than 1mm by using a wood stone (Wood Stone).

In addition, the secondary circulation portion 50 is composed of a circulation space 51, the inner tube 53 and the second outer tube 55, the circulation space 51 is divided into three compartments, The diameter is narrower toward the upper surface is formed, the central portion of the circulation space 51 is made to connect the first outer tube 15 and the inner tube 53.

Here, the inner tube (53) is made to be connected to the first outer tube (15), because when the ultra-mini-foam generator 40 is generated, since the decompression occurs on the lower surface of the inner tube (53) As a result, the suspension liquid mixed with the microorganisms separated from the basal material is introduced into the inner tube 53.

In addition, the second outer tube 55 is formed in a cylindrical shape so as to exceed a predetermined size than the inner diameter of the inner tube 53, so that the circulation space 51 to surround the outer peripheral surface of the second outer tube 55 While being made, the suspension liquid introduced into the second outer tube 55 is separated into three compartments so as to be mixed back into the first outer tube 15, and is formed by decompression from the first outer tube 15. Since the suspension and the suspension flowing into the first outer tube 15 are remixed so as not to mix, the low quality organisms extraction device 1 using the ultra-micropore generator according to the present invention is continuously operated. The solution will circulate.

The microorganisms mixed in the solution flowing under the inner tube 53 are separated from the solution by the ultra-miniature bubbles generated by the ultra-miniature bubble generator.

In addition, the suspension liquid introduced into the inner tube 53 moves to the upper surface of the inner tube 53 while retaining the microorganisms in the ultra-micron cloth, and the air on the upper surface of the second outer tube 55. Bubbles are generated at the water surface in contact with the microparticles, and thus, microorganisms contained in the ultra-micron bubble and floated to the upper surface are attached to the water film of the bubbles.

Thus, some small-sized organic bunnies may be concentrated in the water film, but since the small nitrile particles making up the mud cannot adhere well to the water film, the flow of the solution is precipitated by gravity. As a result of remixing into the first outer tube 15 through the lower portion of the second outer tube, the suspension is generated by the vortex to increase the recovery rate of the microorganisms by the circulation propeller 13.

Here, the sample collection unit 60 is formed to collect microorganisms, is formed in a cylindrical shape to surround the outer surface of the second outer tube 55, is formed to reduce the diameter of the cylinder is formed in a funnel shape , This is a shape for collecting the suspended organisms contained in the bubble.

In addition, the solution is injected into the solution injecting unit 20 into the sample injecting unit 20, which is further provided with a cylindrical shape to surround the outer peripheral surface of the funnel shape to collect the bubbles ejected to the upper surface of the funnel shape. When the boundary surface of the solution rises, bubbles of the upper surface of the second outer tube 55 are collected.

In addition, the sample collection unit 60 is preferably further provided with a circular plate to cover the upper surface of the funnel-shaped and cylindrical collection portion, the sample collection unit 60 of the secondary circulation unit 50 Combined with the upper surface, the amount of the microorganisms and the extraction time can be controlled by the solution injection amount and the flow rate.

Finally, the bubbles flowing into the sample collection unit 60 burst into the cylindrical collection unit to form a solution. A plurality of microorganisms are concentrated in the solution of the sample collection unit 60. It is maintained as clean as possible, it is also possible to move to another container by pipettes, etc. to another container for analysis, it is also preferable to remove the entire sample collection unit 60 to move it to other containers, the above 1 The primary circulation unit 10, the secondary circulation unit 50, the sample collection unit 60, the solution inlet unit 20, and the air pump are removable and movable.

Figure 3 is a schematic diagram of the solution circulation of the microbial microorganism extracting apparatus using the ultra-miniature cell generator according to the present invention, Figure 4 is a flow chart schematically showing the extraction method of the microbial microorganism extracting apparatus using the ultra-miniature cell generator according to the present invention. . As shown in the drawings, the microorganism extracting apparatus using the ultra-miniature gun generator according to the present invention is the first circulation portion and the solvent for the low-quality and seawater solvents such as pearl (Mud) and sand containing microorganisms It begins by injecting a car circulation part (S11).

The solution and the low quality injected into the primary circulation drive a circulation propeller that forms a vortex in the transverse direction so as to increase the recovery rate of microorganisms contained in the low quality, and the first external tube using the circulation propeller. The low quality and the solution is produced as a suspension, and the mixture is continuously mixed until the low quality and the solution is a suspension (S13).

At this time, the circulation direction of the solution is in the direction of ① forming a vortex in the transverse direction in the first outer tube.

Here, the air pump is operated to operate the ultra-fine foam generator, which is formed of a wooden stone on the lower surface of the inner tube through the air injection tube inserted along the inner tube of the secondary circulation part to form an ultra-small foam of 1 mm or less ( S15).

In addition, as the ultra-low intensity gun rises in the inner tube, a step down occurs in the inner tube lower surface, whereby an action of raising the suspension liquid generated in the first outer tube to the inner tube lower surface occurs, thereby The direction of movement is a direction? Perpendicular to the upper surface moving from the first outer tube to the circulation space portion, the suspension liquid flows into the inner tube, and the solution from the first outer tube toward the inner tube is direction ③. (S17).

In addition, the suspension flowed into the inner tube is moved to the upper surface of the inner tube which is the inner cylindrical tube having a small diameter of the secondary circulation portion by the ultra-mini strength gun, the direction of the solution movement is ④ direction, While mixed with the ultra-miniature foam, the bubbles contain microorganisms in the suspension.

And, when the foam is in contact with the water surface in contact with the air on the upper surface of the second outer tube formed on the outer surface of the inner tube, the poor quality that did not adhere to the water film (Great Film) again by the second outer tube It flows into the lower surface of the circulation direction from the water surface of the upper surface of the second outer tube back to the lower direction of the second outer tube is ⑤ direction.

Thus, the suspension mixed with the reservoir and the solution flowing into the lower surface of the second outer tube is lowered, and the solution direction thereof is ⑥, and the primary circulation with the lower surface of the second outer tube of the circulation space formed of three compartments. Re-mixing with the primary circulation through the pipe connecting the first outer pipe of the negative, according to the solution flow direction is ⑦ (S19).

In addition, below the lower end of the lower surface of the sample injection unit, the solution is injected through the solution injection unit to a certain height of the secondary circulation unit (S21), and when the suspension comes into contact with the water surface, which is the upper surface of the secondary circulation unit, bubbles As the amount of foam generated increases as the solution containing the sea water is injected, the level of the foam is controlled to prevent the foam from flowing into the sample injection unit for a predetermined time so that substances other than the microorganisms of low quality are dropped by gravity (S23). .

In addition, after a certain period of time, the small nitrile particles constituting the pearl (Mud) is sufficiently precipitated by gravity and is pushed downward along the direction of the solution ⑥, ⑦, slowly injecting the solution into the solution injector Increasing the solution to collect the bubbles on the upper surface to the sample collection while increasing the water level as the interface, and collects the bubbles in the sample collection by slowing the flow rate (S25).

Thus, the collected foam is transformed into a solution of the foam in the sample collection unit, which is a plurality of microorganisms are concentrated, so that the sample is collected and extracted (S27).

FIG. 5 is a perspective view schematically showing a primary circulation unit of an extract of low quality organisms using an ultra-miniature gun generator according to the present invention. As shown in the figure, the primary circulation portion 10 is composed of a circulation propeller switch 11, a circulation propeller 13, and a first outer tube 15.

Here, the circulation propeller switch 11 is turned on and is electrically connected to drive the circulation propeller 13 when a constant current is applied thereto.

In addition, the circulation propeller 13 forms a vortex in the transverse direction inside the primary circulation portion 10 to form a solution and a low quality suspension, so that a large number of suspended organisms can be contained in the suspension.

Figure 6 is a perspective view schematically showing a solution injecting portion of the low quality biological extracting apparatus using the ultra-miniature foam generator according to the present invention. As shown in the figure, the solution injecting unit 20 injects a salinity solution including seawater using the principle of the piston.

Then, the solution moves through the solution injection tube 21, and the solution is injected directly into the first outer tube of the primary circulation through the secondary circulation.

Figure 7 is a schematic exploded perspective view and cross-sectional view of the secondary circulation portion of the low quality biological extracting apparatus using the ultra-miniature gun generator according to the present invention. As shown in the figure, the secondary circulation portion 50 has an inner tube 53 directly connected to the first outer tube of the primary circulation portion, and has an inner diameter increased by a predetermined length than the inner tube 53. By connecting the second outer tube 55, the lower surface of the second outer tube 55 and the first outer tube of the primary circulation portion to enable the flow in the lower direction, the first outer tube and the inner tube (53) It includes a circulation space 51 is formed to be divided into three compartments to connect the lower surface of the () to enable the flow in the upper direction.

Each of the above components can be assembled.

Figure 8 is a perspective view of the air pump and the ultra-mini-foam generator of the microbial extraction apparatus using the ultra-mini-foam generator according to the present invention. As shown in the figure, the air pump 30 according to the present invention is provided to move the air at a constant pressure, and thereby the air injection tube 31 and the air injection is a passage through which the moved air is moved It consists of an ultra-mini-foam generator 40 made of Wood Stone is mounted to the end of the dragon tube (31).

The ultra-mini-foam generator 40 may generate an ultra-mini-foam gun of 1 mm or less, and the ultra-mini-foam generator 40 is provided inside the inner tube of the secondary circulation portion and mixed in the first outer tube. Decompression occurs to raise the fluid to the inner tube.

9 is an exploded perspective view, cross-sectional view and a front view of the sample collection unit of the microbial extraction apparatus using the ultra-miniature foam generator according to the present invention. As shown in the figure, the sample collection unit 60 is formed to surround the upper surface of the second outer tube, it is made of a funnel shape so that bubbles generated from the upper surface flows out.

And, when the bubble is blown out in the shape of the funnel, a cylindrical round collecting portion is formed to form it as a solution, is formed in a cylindrical shape of a certain length increased than the second outer tube, covering the upper surface of the cylindrical shape It is also preferable that a circular plate is formed.

Lastly, the present invention can be manufactured in a low-quality biological extracting device using the ultra-miniature cannon generator, portable, because it is light and small in size of 40cm in height, 12cm in width, 1.2kg in weight, easy to carry, the power source is a common battery AA size (separation device 2EA, aeration device 2EA) can be used in the field easily, the size can be changed depending on the application.

At this time, each of the components are made of a transparent acrylic material it is possible to visually check the circulation of seawater and the separation of organisms, it is preferable to be made to determine whether the malfunction of the machine.

In addition, this portable device can directly process samples sampled on-site, thereby reducing the time taken to the lab and thus processing samples sampled directly, thus maintaining the freshness of the sample. Can save time.

<First Embodiment>

After assembling all the components of the microbial extracting apparatus using the ultra-micropore generating apparatus according to the present invention, a solution of 1.2L of seawater having a concentration of 28 ‰ was used, and 10g of mud was injected into the pulp. Thereafter, the circulation propeller is operated to rotate the bottoms and the solution laterally to form a turbid liquid.

Then, after 1 minute, when the low quality and the solution are mixed, the solution is slowly injected through the solution inlet until the height of the seawater solution reaches 1 cm below the sample collection end.

So, the bubble level generated from the surface of the solution is controlled to prevent water from flowing into the sample collection unit for 15 minutes, and after 15 minutes, the flow rate and flow rate are gradually injected into the solution inlet unit, and the foam is introduced into the sample inlet unit. A suitable flow rate and flow rate for the 10-20 ml solution to collect in the sample collection section is appropriate.

In addition, when the solution of about 10-20ml is collected in the sample inlet, the solution is transferred to a container that can be easily observed under a microscope such as petri-dish to determine the number of nematodes, which are a group of linear animals. The nematode is a microorganism having a size of about 30-250um and most inhabiting in the vagina, and is easily used as an indicator organism for recovery because it is easy to identify aberrations through microscopic observation.

Here, Table 1 shows the biomass recovered and the contamination of the poor constituents according to the bubble type.

In addition, as a result of repeating three experiments, an average of 224 ± 57 nematodes were identified in 10 g of pearl, and after observation, the solution was filtered through a filter of 0.45 um to confirm the dry weight, and the results of three repeated experiments were 0.0435 ± 0.0083 g.

Here, when the same experiment three times with the existing air generator (Air-Stone), the number of nematodes was 71 ± 24, the dry weight is 0.0660 ± 0.0067g, the case of using the ultra-miniature foam generators nematode ash It can be seen that the yield increased by 3.15 times, and the dry weight decreased by 1.52 times.

This is an embodiment that shows that the use of ultra-mini-foamed bubbles, the biological recovery is higher than the existing bubbles, and the turbidity of the recovered solution is reduced, so that microorganisms can be effectively recovered.

In addition, Table 2 is a table showing the number of nematodes recovered after the addition of 5% artificial sea salt.

Here, the concentration of seawater used as a solution was added 5% of artificial sea salt or magnesium chloride (MgCl ₂ ), and the same experiment was performed twice to confirm the number of nematodes. The number of nematodes remaining in the larvae was 25 ± 7, and the microbial recovery rate of the sample collection unit was 95.7% or more.

In addition, it is an ultra-mini-foam generator that can generate bubbles with Wood Stone or Limewood Air Stone, etc., to generate bubbles with a bubble size of 1 mm or less, further increasing the floatation force of microorganisms, The amount of microorganisms to be extracted is increased, and the residual material residues in the collected solution are reduced, so that cleaner microorganisms can be extracted from the low quality.

Second Embodiment

FIG. 10 is a view illustrating bubble generation in the case of using wood and air stones in fresh water and sea water. As shown in the figure, FIG. 10A is a case where a general air stone is used in fresh water, and FIG. 10B is a case where a wood stone, which is an ultrafine micropore generator, is used in fresh water.

The low quality material extraction apparatus using the ultra-micron bubble generator according to the present invention does not generate ultra-miniature bubbles in a low concentration solution including fresh water or microbial culture medium, and generates bubbles that are almost the same as the existing ultra-miniature bubble generators.

In addition, since the bubbles that can overflow in the low-quality material extraction device using the ultra-miniature foam generator according to the present invention does not form the biological collection using bubbles to the collecting unit, the device of the present invention is low in fresh water or microbial culture medium For normal operation of the solution, salts such as sea salt should be added to increase the concentration of the solution.

FIG. 10C illustrates a case where a general air stone is used in seawater, and FIG. 10D illustrates a case where a wood stone, which is an ultrafine micro-foam generator, is used in seawater. As shown in the figure, it can be seen that the difference between the general bubble and ultra-miniature bubbles is generated when a fine stone is used in the wood stone of ultra-high strength bubbles in a high concentration solution such as seawater.

Therefore, in order to operate normally it is necessary to add salts and salts to increase the solution, it can be seen that more bubbles are generated when using the ultra-micro foam gun.

Third Embodiment

Table 3, which operates the low-quality organisms extracting apparatus using the ultra-miniature cannon generator according to the present invention, and measures the number of nematodes recovered by time zones is as follows.

During the three repetitions, it can be seen that the recovery rate of microorganisms is 78.9% within 9 minutes and 92.3% within 20 minutes after operating the microorganism extracting device using the ultra-micron cell generator according to the present invention. .

Table 4 shows the dry weight of the residue contained in the solution according to Table 3, as follows.

Here, the solution introduced into the sample collection unit was first filtered to 62 μm and subjected to secondary filtering to 0.45 μm to check dry weight.

And, it can be seen that only residues of 2.5% or less per time zone are included in the collected solution. Accordingly, since the amount of residues in the solution is small, it can be seen that the microscopic observation is very easy.

In addition, Figure 11 is a photograph taken with a 0.45um filter containing the residue used in the dry weight check by performing the above experiment using the microbial extraction apparatus using the ultra-miniature gun generator according to the present invention.

Here, it can be seen that very little residue remained in the filter of the collected solution as compared to the final residue filter remaining in the microbial extraction apparatus using the ultra-micropore generator.

Although the preferred embodiments of the present invention have been described above by way of example, the scope of the present invention is not limited to such specific embodiments, and those skilled in the art are appropriate within the scope described in the claims of the present invention. It will be possible to change.

As described above, the present invention having the configuration as described above is provided with a circulating propeller to form a vortex in the transverse direction, thereby increasing the recovery rate of microorganisms from the suspension mixed with lipids and the solution, and ultra-fine foam of 1 mm or less By forming a rising flow using a wood stone or limewood air stone to form a swell, the flotation of the microorganisms is increased, thereby rapidly increasing the amount of microorganisms that are transferred to the sample collection unit, and the residual amount is reduced to a clean state. Microorganisms can be observed, and extraction time and amount can be controlled by using seawater injector for qualitative and quantitative analysis of low quality organisms. It can achieve such effects.

Claims (7)

A primary circulation part 10 for generating and raising a suspension by mixing the solution injected by the solution injecting part 20 with the low quality; The secondary circulation portion which is located above the primary circulation portion 10 and floats the suspension raised from the primary circulation portion 10 with an ultra-fine strength bubble of 1 mm or less generated by the ultra-fine force gun generator 40. 50; And An apparatus for extracting microorganisms of a low quality microorganism using an ultra-miniature cell generator which is located above the secondary circulation unit 50 and includes a sample collection unit 70 for collecting microorganisms suspended by the secondary circulation unit 50. . The method of claim 1, The secondary circulation unit 50 is An inner tube (53) to which the suspension of the primary circulation portion (10) is combined with the ultra-miniature cloth; A second outer tube (55) in which the suspension combined in the inner tube (53) is moved along the upper and outer surfaces of the inner tube with the rise of the ultra-low intensity gun; And a circulation space portion 51 coupled to the inner tube to move the suspension of the primary circulation portion and to connect the lower surface of the second outer tube to remix the suspension into the primary circulation portion. Low quality microbial extraction device using an ultra-miniature gun generator. (delete) The method of claim 1, The extraction amount and the extraction time of the microorganisms extracted from the sample collection unit, the low-quality microbial extractor using the ultra-miniature foam generator, characterized in that it is adjusted according to the solution injection amount and flow rate injected through the solution injection unit 20 . The method of claim 1, In order to increase the recovery rate of microorganisms in the reservoir, the primary circulation unit 10 includes a circulation propeller 13 for continuously mixing the reservoir and the solution. . The method of claim 1, In order to increase the microbial recovery rate, by adding a salt to the solution injected through the solution injector 20, salt concentration (Salt Concentration) of the solution, characterized in that to increase the microporous microorganism extractor. The method of claim 1, The first microcirculation unit, the second circulation unit, the sample collection unit and the solution injection unit is a microscopic microorganism extraction apparatus using a micro-miniature foam generator, characterized in that the removable.

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