KR100799937B1 - Culture equipment for micropropagation and acclimatization in plantlets cultured in vitro - Google Patents

Abstract

The present invention is a culture apparatus for collecting and culturing the seedlings of the seedlings, and more specifically, the seedlings of the seedlings are harvested and cultured in a sterile culture tank to grow, rooting, and purifying the seedlings continuously in the culture tank. The present invention relates to a culture apparatus for purifying seedling plant growth that provides a culture environment.

The configuration of the present invention, the culture tank of the transparent material to collect and culture the seedlings of the seedlings, and the rim is composed of a rubber magnet seated on the upper culture tank to prevent the penetration of bacteria and mold to maintain the inside of the culture tank aseptic state The culture vessel cover of the transparent material, and the culture vessel cover is completely sealed with the culture tank to seal effectively made of a rubber magnet on the top of the culture tank to effectively block the infiltration of bacteria and fungi, and the inner working in the culture tank is possible An open door provided at one side of the culture tank, a culture medium tank for supplying a culture solution to promote growth of seedlings cultured in the culture tank, and a culture solution formed at one side of the culture tank to form a culture solution in the culture tank. A culture solution inlet to be supplied to the culture medium, and a culture solution outlet formed at the other side of the culture tank to discharge the culture solution. A first air supply port for supplying the air required for the growth of the seedling plant from the outside of one side of the culture tank, and an air outlet for discharging the external air supplied from the first air supply to the other side of the culture tank, the inside of the culture tank A first air aseptic filter provided at the first air supply port to which external air is supplied to prevent contamination of the air, and a second air aseptic filter provided at the air outlet to remove bacteria and mold in the air discharged from the air outlet. A filter, a first liquid sterile filter provided at the culture inlet for supplying the culture solution aseptically, and a second liquid sterile filter provided at the culture outlet to filter the culture solution discharged from the culture outlet and to be discharged aseptically. And an infrared sterilization lamp for sterilizing and supplying the culture solution supplied to the culture tank, and at the upper end of the culture tank. Electrical conductivity (Electrical Conductivity: EC), the pH (Hydrogen Ion Concentration: pH) and by measuring the temperature of the broth culture contamination detection unit consisting of a sensor for detecting whether the contamination of the culture liquid; The culture medium discharged from the culture medium discharge port is circulated to the culture medium tank through the culture medium circulation port, the first motor for circulating and supplying the culture medium in the culture medium tank to the culture tank through the culture medium inlet, and the seedlings cultured in the culture tank When the culture medium is detected by the bacteria and mold by the contamination detection sensor provided in the culture tank and the second motor for supplying the air necessary for growth from the outside and supplied to the culture tank through the first air supply port A pollution alarm device that generates an alarm sound to notify the contamination of the light, a light quantity control device that controls the amount of light according to the growth and photosynthesis of the seedlings, and the first and second motors, the pollution alarm device, and the light quantity control device And integrated control device consisting of a display unit capable of checking the operating state; A ventilation amount adjusting device configured to circulate the external air supplied from the first air supply unit in the culture tank to directly supply to the roots and stems of the seedlings to promote the growth of the seedlings; And an illuminance control device positioned at an upper portion of the culture vessel cover to directly supply the light controlled by the light quantity control device to the seedlings according to the amount of light required for photosynthesis of the seedlings to promote the growth of the seedlings. It is done.

Culture Tank, Seedling Plant, Culture Tank

Description

CULTURE EQUIPMENT FOR MICROPROPAGATION AND ACCLIMATIZATION IN PLANTLETS CULTURED IN VITRO}

1 is an exploded perspective view showing a culture apparatus for purifying seedlings growth according to the present invention,

Figure 2 is a combined perspective view showing a culture apparatus for purifying seedling plant growth according to the present invention,

Figure 3 is a perspective view showing an integrated control device of the culture apparatus for purifying seedling plant growth according to the present invention,

4 is a cross-sectional view of the culture medium inlet port of the culture apparatus for purifying seedlings growth according to the present invention,

Figure 5 is a cross-sectional view of the culture medium outlet of the culture apparatus for purifying seedlings growth according to the present invention,

Figure 6 is an exploded perspective view showing a ventilation amount control device of the culture apparatus for purifying seedlings growth according to the present invention,

7 is a perspective view showing a ventilation amount control device of the culture apparatus for purifying seedlings growth according to the present invention,

8 is a perspective view showing a state in which the forming tray is placed on the ventilation amount control device of the culture apparatus for purifying seedlings growth according to the present invention,

Figure 9 is a cross-sectional view showing the roughness control device of the culture apparatus for purifying seedlings growth according to the present invention,

10 is a cross-sectional view showing the lift of the roughness control device of the culture apparatus for purifying seedlings growth according to the present invention,

11 is a cross-sectional view showing another embodiment of the roughness control device of the culture apparatus for purifying seedlings growth according to the present invention,

12 is a cross-sectional view showing that the roughness control device of another embodiment of the culture apparatus for purifying seedlings growth according to the present invention.

<Description of Symbols for Major Parts of Drawings>

100: culture unit

105: culture tank 110: culture tank cover

115: culture tank cover handle 120: sealed packing

125: culture medium tank 130: culture solution inlet

135: first liquid aseptic filter 140: culture solution outlet

145: second liquid aseptic filter 150: first air supply port

155: first air aseptic filter 160: air outlet

165: second air aseptic filter 170: pollution detection sensor

175: infrared sterilization lamp 180: open door

200: integrated control device

210: first motor 220: second motor

230: pollution alarm device 240: light quantity control device

250: culture medium circulation port 260: display unit

300: ventilation amount control device

305: second air supply port 310: seating step

315: air supply unit 320: first porous plate

325: first porous tool 330: first cotton fiber

335: second porous plate 340: second porous tool

345: second cotton fiber 350: third porous plate

355: 3rd tool 360: molding tray

400: illuminance control device

410: light emitting unit 420: small fan

430: light panel 440: lower support

450: lifting cylinder 460: top plate

470: driving handle 480: spiral lifting bar

The present invention is a culture apparatus for collecting and culturing the seedlings of the seedlings, more specifically, the seedlings of the seedlings are cultured in a sterile culture tank to grow, rooting, purifying the seedlings can be continuously made in the culture tank. The present invention relates to a culture apparatus for purifying seedling plant growth.

In general, the growth of conventional seedlings is carried out through various processes such as growth point culture, cultured plant induction, proliferation, rooting induction, purifying, etc., because each process is performed separately, the work efficiency is reduced, and the growth of seedlings There was a problem of being delayed.

In addition, after each process, the seedlings can not be preserved in a sterile state, there is a problem that the survival rate of the seedlings is reduced due to exposure to bacteria.

The present invention has been made in order to solve the problems of the prior art, the main object of the present invention is to take a slice of the seedlings and incubate in a sterile culture tank, the growth, rooting, and purification of the seedlings can be continuously made in the culture tank. It is an object of the present invention to provide a culture apparatus for purifying seedling plant growth.

In order to achieve the above object, the culture tank of a transparent material for collecting and cultivating the slices of the seedlings, and the rim is composed of a rubber magnet seated on the top of the culture tank to prevent the penetration of bacteria and fungi to aseptic culture tank A culture tank cover of a transparent material to maintain the inside, and a sealed packing made of a rubber magnet on the top of the culture tank so that the culture tank cover is completely sealed with the culture tank to effectively block the penetration of bacteria and fungi, and the inside of the culture tank An open door provided at one side of the culture tank to enable work, a culture medium tank for supplying a culture solution to promote the growth of seedlings cultured in the culture tank, and a culture medium formed at one side of the culture tank so that the culture solution is in the culture tank. A culture solution inlet supplied into the culture tank, and formed on the other side of the culture tank to discharge the culture solution The culture medium discharge port, and the first air supply port for supplying the air required for the growth of the seedling plant on one side of the culture tank from the outside, the air outlet for discharging the external air supplied from the first air supply to the other side of the culture tank; A first air aseptic filter provided at the first air supply port to which external air is supplied to prevent contamination of the culture tank, and provided at the air outlet to remove bacteria and mold in the air discharged from the air outlet. A second air sterile filter, a first liquid sterile filter provided in the culture solution inlet for supplying the culture solution in a sterile state, and a second solution provided in the culture solution outlet so as to filter and discharge the culture solution discharged from the culture solution outlet in a sterile state. A two-liquid aseptic filter, an infrared sterilization lamp for sterilizing and supplying the culture solution supplied to the culture tank, and the vessel The top of the solution tank, the electrical conductivity (Electrical Conductivity: EC), the pH (Hydrogen Ion Concentration: pH), and the culture unit to measure the temperature of the culture medium consisting of contamination detecting sensor for detecting whether the contamination of the culture liquid; The culture medium discharged from the culture medium discharge port is circulated to the culture medium tank through the culture medium circulation port, the first motor for circulating and supplying the culture medium in the culture medium tank to the culture tank through the culture medium inlet, and the seedlings cultured in the culture tank When the culture medium is detected by the bacteria and mold by the contamination detection sensor provided in the culture tank and the second motor for supplying the air necessary for growth from the outside and supplied to the culture tank through the first air supply port A pollution alarm device that generates an alarm sound to notify the contamination of the light, a light quantity control device that controls the amount of light according to the growth and photosynthesis of the seedlings, and the first and second motors, the pollution alarm device, and the light quantity control device And integrated control device consisting of a display unit capable of checking the operating state; A ventilation amount adjusting device configured to circulate the external air supplied from the first air supply unit in the culture tank to directly supply to the roots and stems of the seedlings to promote the growth of the seedlings; And an illuminance control device positioned at an upper portion of the culture vessel cover to directly supply the light controlled by the light quantity control device to the seedlings according to the amount of light required for photosynthesis of the seedlings to promote the growth of the seedlings. It is done.
In addition, the ventilation amount control device, the second air supply port is installed in the culture tank and connected to the first air supply port and the air supply unit is provided with a seating step therein; The first porous plate is composed of a material which is seated on the seating step of the air supply unit and is not corroded by the culture medium, and absorbs the first porous plate through which the air is discharged to the first porous tool, and the culture solution supplied to the air supply unit. The first surface fiber which is in close contact with the upper part of the first porous plate and keeps the amount of air discharged to the first porous tool constant, and is laminated in series with the first porous plate. The second porous tool is alternately arranged to absorb air from the second porous plate to discharge the air into the second porous tool, and the culture medium supplied to the air supply unit is in close contact with the upper portion of the second porous plate and discharged to the second porous tool. A second porous plate having a second surface fiber which maintains a constant amount of air, and a third porous plate which is stacked on top of an air supply unit subsequent to the second porous plate to form a third porous tool in the same manner as the first and second porous tools are formed Made up of And a gong.
In addition, the illuminance control device is located in the upper portion of the culture tank cover, the light emitting unit for emitting the light controlled by the light quantity control device according to the amount of light required for photosynthesis of the seedlings, and removes the heat generated in the light emitting unit An optical panel comprising a plurality of small fans, a lower support configured to support the load of the optical panel, a lifting cylinder inserted / formed into the lower support and configured to penetrate the optical panel, and the optical A top plate which is provided at an upper end of the panel and connected to the lifting cylinder, and a driving handle which generates a driving force by rotation on one side of the upper plate, and the lifting cylinder and the optical panel are lifted due to the driving of the driving handle. It is done.
In addition, the lifting cylinder, the spiral lifting bar is inserted into the lower support and the spiral lifting bar is formed in the lower support engaging with the spiral formed in the lower support, the spiral lifting bar and the optical panel is driven by the driving handle It is characterized by being elevated.

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Hereinafter, the configuration of the present invention will be described in detail with reference to the accompanying drawings.

1 is an exploded perspective view showing a culture apparatus for purifying seedlings growth according to the present invention, Figure 2 is a combined perspective view showing a culture apparatus for purifying seedlings growth according to the present invention, Figure 3 is a seedling growth according to the present invention Figure 4 is a perspective view showing an integrated control device of the culturing apparatus for purifying, Figure 4 is a cross-sectional view of the culture medium inlet port of the culturing apparatus for seedling growth propagation according to the present invention, Figure 5 is a culture medium of the culture apparatus for purifying seedlings growth according to the present invention 6 is an exploded perspective view showing a ventilation amount control device of the seedling growth medium purifying apparatus according to the present invention, Figure 7 is a combination showing a ventilation amount control device of the seedling growth growth apparatus according to the present invention. Fig. 8 is a perspective view showing a state in which a forming tray is placed on the ventilation amount control device of the culture apparatus for purifying seedling growth according to the present invention. 9 is a cross-sectional view showing the roughness control device of the culture apparatus for purifying seedlings growth according to the present invention, Figure 10 is a cross-sectional view showing the lift of the roughness control device of the culture apparatus for purifying seedlings growth according to the present invention. 11 is a cross-sectional view showing another embodiment of the roughness control apparatus of the seedling growth propagation apparatus according to the present invention, Figure 12 is a roughness control apparatus of another embodiment of the seedling growth propagation apparatus according to the present invention. It is sectional drawing which shows lifting.

As shown in Figures 1 to 12, in order to achieve the above object, a transparent tank cultivation tank 105 for cultivating the seedlings by culturing the seedlings of the seedlings is provided, the frame is composed of a rubber magnet It is seated on the culture tank 105 to prevent the penetration of bacteria and mold is provided with a culture tank cover 110 of a transparent material to maintain the inside of the culture tank 105 in a sterile state.

At this time, the culture tank cover 110 is completely sealed with the culture tank 105 is provided with an airtight packing 120 made of a rubber magnet on the top of the culture tank 105 to effectively block the penetration of bacteria and fungi between the magnets Due to the magnetic force, the culture vessel 105 and the culture vessel cover 110 are completely sealed to effectively block the infiltration of bacteria and fungi, thereby maintaining the inside of the culture vessel 105 in a sterile state.

In particular, the culture tank cover 110, the upper end of the culture tank cover handle 115 is provided so that the operator can easily remove and install the culture tank cover 110.

In addition, an open door 180 is provided at one side of the culture tank 105 to enable internal work in the culture tank 105, and the culture tank 105 is maintained in a sterile state without opening the culture tank cover 110. You can work in one state.

In this case, in order to maintain the culture tank 105 in a sterile state, it is preferable that the worker sterilize the worker's hand with alcohol and then perform the internal work of the culture tank 105.

In addition, there is provided a culture medium tank 125 for supplying a culture solution to promote the growth of seedlings cultured in the culture tank 105, wherein the culture medium tank 125 is a bioreactor for preserving and supplying the culture in a sterile state It is preferable to use as a culture tank (125).

 In particular, one side of the culture tank 105 is provided with a culture solution inlet 130 for supplying the culture solution from the culture tank 125, the other side of the culture tank 105, the culture solution outlet 140 for discharging the culture solution It is equipped with a culture solution is circulated / supplied.

In addition, one side of the culture tank 105 is provided with a first air supply port 150 for supplying the air required for the growth of the seedlings, and the other side of the culture tank 105, the air outlet for discharging the supplied air ( 160 is provided, the air in the culture tank 105 is circulated to supply and discharge the air required for the growth of the seedlings cyclically to promote the growth of the seedlings.

In particular, a first air aseptic filter 155 is provided at the first air supply port 150 through which air is supplied to prevent contamination in the culture tank 105, thereby removing the bacteria and fungi present in the air, thereby culturing the culture. Aseptic air is supplied to the tank 105.

In addition, to remove the bacteria and mold in the air discharged from the air outlet 160 A second air sterile filter 165 is provided, and the first liquid sterile filter 135 is provided at the culture solution inlet 130 to supply the culture solution aseptically.

In addition, the second liquid aseptic filter 145 for filtration of the culture liquid discharged from the culture liquid discharge port 140 and discharged in a sterile state, it is possible to circulate / supply the sterile culture liquid.

In addition, an infrared sterilization lamp 175 is provided to sterilize the culture solution supplied to the culture tank 105 and supply the sterilized culture solution to the culture tank 105, and the electrical conductivity (at the top of the culture tank 125) is provided. Electrical Conductivity (EC), Hydrogen Ion Concentration (pH) and the culture unit 100 consisting of a pollution detection sensor 170 for detecting the contamination of the culture medium by measuring the temperature of the culture medium.

In addition, the integrated control device 200 capable of integrated control of the culture unit 100 circulates the culture solution discharged from the culture medium outlet 140 to the culture medium tank 125 through the culture medium circulation port 250, the culture medium tank The first motor 210 for supplying the culture solution in the 125 to the culture tank 105 through the culture solution inlet 130 is provided, and the air necessary for the growth of the seedlings cultured in the culture tank 105 is provided. The second motor 220 is provided to the culture tank 105 through the first air supply port 150, wherein the first and second motors 210 and 220 are rotated by a motor using a magnet pump capable of quantitative supply. It is preferable to use water, and it is also preferable to supply culture medium and air using a solenoid valve.

In addition, a contamination alarm device 230 is provided by the contamination detection sensor 170 provided in the culture tank 125 to generate an alarm sound to notify the contamination of the culture medium when the culture medium is detected by the bacteria and mold. And the light quantity control device 240 for controlling the light quantity according to the growth of the seedlings, the first and second motors 210 and 220, the pollution warning device 230, and the light quantity control device 240 in an integrated state. It consists of a display unit 260 that can be confirmed.

In addition, it is configured inside the culture tank 105 to supply external air supplied from the first air supply unit 150 directly to the roots and stems of the seedlings in the culture tank 105 to promote the growth of the seedlings. Ventilation amount control device 300 is configured, the ventilation amount control device 300 is installed in the culture tank 105, the second air supply port 305 is installed in series with the first air supply port 150. Is provided, and consists of an air supply unit 315 is provided with a seating step 310 therein.

In particular, the first porous plate 320 is composed of a material which is seated on the seating step 310 and is not corroded by the culture medium is configured to discharge the air to the first porous tool 325 Is provided, the first surface fiber absorbs the culture solution supplied to the air supply unit 315 is in close contact with the upper portion of the first porous plate 320 to maintain a constant amount of air discharged to the first porous tool 325 330 is provided.

 In addition, the second porous tool 340 is configured to be successively stacked on the first porous plate 320 and alternately with the first porous tool 325 of the first porous plate 320 to form the second porous tool 340. ) Is provided with a second porous plate 335 for discharging air.

In addition, the second surface fiber 345 to absorb the culture solution supplied to the air supply unit 315 to be in close contact with the upper portion of the second porous plate 335 to maintain a constant amount of air discharged to the second porous tool 340. And a third porous tool 355 which is stacked on top of the air supply unit 315 following the second porous plate 335 to form the first porous tool 325 and 340. The three-perforated plate 350 is configured to supply the air necessary for the growth of the seedlings, and finally, the molding tray 360, which is obtained by cutting the seedlings of the seedlings, is seated on the third perforated plate 350. The air discharged from the porous tool 355 is supplied.

At this time, it is preferable that the air supplied to the lower end of the forming tray 360 is effectively delivered to the pieces of the seedlings.

In addition, the number of the first and second porous tools 325 and 340 of the first and second porous plates 320 and 335 is maintained at 200, and the porous holes are alternately used, and the third porous tool of the third porous plate 350 is used. Maintaining the number of 355 to 400 The seedlings which should be formed in the same manner as the formation of the first and second porous tools 325 and 340 to supply air to the roots are used on the seedlings using the first and second porous plates 320 and 335. It is preferable that the seedlings that supply air and supply air to the roots and stems are supplied with air to the seedlings using all of the first, second, and third porous plates 320, 335, and 350.

In addition, the first and second surface fibers 330 and 345 in close contact with the first and second porous plates 320 and 335 are in close contact with the first and second porous plates 320 and 335 without a gap in a state in which the liquid is absorbed. At the same time the same amount of air is discharged from the three multi-tool (325, 340, 355).

In addition, according to the amount of light required for photosynthesis of the seedlings is provided with an illumination control device 400 for directly supplying the light controlled by the light amount control device 240 to the seedlings to promote the growth of the seedlings.
In addition, the illuminance control device 400 is positioned above the culture vessel cover 110, the light emitting unit 410 for emitting light controlled by the light amount control device 240 according to the amount of light required for photosynthesis of the seedlings. And an optical panel 430 composed of a plurality of small fans 420 to remove heat generated from the light emitting unit 410.
In particular, since the light emitting unit 410 directly supplies the light required for photosynthesis of the seedlings to the seedlings, the growth of the seedlings is promoted.
In addition, the lower support 440 is provided to support the load of the optical panel 430, the lifting cylinder 450 which is inserted / formed into the lower support 440 and penetrates the optical panel 430 ) Is provided.

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In particular, the upper panel 460 is provided on the top of the optical panel 430 is configured to be connected to the lifting cylinder 450, the driving handle for generating a driving force by the rotation on one side of the upper plate 460 470 is provided, and the lifting cylinder 450 and the light panel 430 are lifted due to the driving of the driving handle 470.

In addition, in place of the elevating cylinder 450, the spiral is formed by using a spiral elevating bar 480 is engaged with the spiral formed in the lower support 440 is inserted / formed in the lower support 440. Thus, it is preferable that the driving handle 470 is driven so that the spiral lifting bar 480 and the optical panel 430 are elevated.

At this time, the light amount of the light emitting unit 460 is controlled through the light amount control unit 240 of the integrated control device 200.

In addition, the roughness control device 400 is raised 0 ~ 30cm it is preferable to cultivate the seedlings by adjusting according to the growth stage of the seedlings.

In addition, it is preferable to connect and control the integrated control device 200 and the personal computer, record and preserve the measured and measured results, and edit and store them using a separate software program.

Although specific embodiments of the present invention have been described and illustrated above, it will be apparent that the present invention may be modified and practiced by those skilled in the art.

Such modified embodiments should not be individually understood from the technical spirit or the prospect of the present invention, and such modified embodiments should fall within the scope of the appended claims.

According to the present invention as described above, since the various processes such as growth point culture, culture plant induction, growth, rooting induction, purification of the seedlings is made in the culture tank, there is an effect of increasing the work efficiency.

In addition, since the seedlings are cultured in a sterile culture tank without undergoing each process, the growth and survival rate of the seedlings is remarkably improved.

In addition, by supplying the appropriate amount of air required for the growth of the seedlings through the ventilation control device and by ventilating the air in the culture tank has the effect that the growth time of the seedlings is shortened and the survival rate is improved.

In addition, since the roughness is adjusted according to the growth stage of the seedlings using a roughness control device capable of lifting, there is an effect that the growth of the seedlings in the culture tank is promoted.

In addition, the integrated control device and personal computer are connected to record and preserve the measured and measured results, and they can be edited and stored using a separate software program, making it possible to calculate data ideal for the growth of seedlings. It can shorten the period and create an environment for mass production of seedlings.

Claims (4)

In the culture apparatus for collecting and cultivating the slices of the seedlings, The culture tank 105 of a transparent material for collecting and cultivating the seedlings of the seedlings and the rim is composed of a rubber magnet and seated on the culture tank 105 to prevent the penetration of bacteria and mold to prevent the infiltration of bacteria and fungi (105). Culture tank cover 110 of the transparent material to maintain the inside, and the culture tank cover 110 is completely sealed with the culture tank 105 to the top of the culture tank 105 to effectively block the penetration of bacteria and fungi An airtight packing 120 made of a rubber magnet, an open door 180 provided at one side of the culture tank 105 to enable internal work in the culture tank 105, and culture in the culture tank 105. A culture solution tank 125 for supplying a culture solution to promote growth of seedlings to be grown, and a culture solution formed in one side of the culture tank 105, and the culture solution supplied from the culture tank 125 to the culture tank 105. Injection port 130 and the other of the culture tank 105 The culture medium discharge port 140 formed on the side and the culture medium is discharged, and the first air supply port 150 for supplying air necessary for the growth of the seedlings from the outside to one side of the culture tank 105, and the culture tank ( On the other side of the 105, the air discharge port 160 through which the external air supplied from the first air supply unit 150 is discharged, and the first air supply to which external air is supplied to prevent contamination of the inside of the culture tank 105. The first air aseptic filter 155 provided in the sphere 150, and the second air aseptic filter 165 provided in the air outlet 160 to remove bacteria and mold in the air discharged from the air outlet 160 ), And the first liquid sterile filter 135 provided in the culture solution inlet 130 and the culture solution discharged from the culture solution outlet 140 in order to supply the culture solution in a sterile state to discharge the culture solution in a sterile state. Second liquid sterile provided in the outlet 140 145, an infrared sterilization lamp 175 for sterilizing and supplying the culture solution supplied to the culture tank 105, and an electrical conductivity (EC) and a hydrogen ion concentration at the upper end of the culture tank 125. (Hydrogen Ion Concentration: pH) and the culture unit 100 consisting of a contamination detection sensor 170 for detecting the contamination of the culture medium by measuring the temperature of the culture medium; The culture medium discharged from the culture medium discharge port 140 is circulated to the culture medium tank 125 through the culture medium circulation port 250, and the culture medium in the culture medium tank 125 is passed through the culture medium inlet port 130. The first motor 210 for circulating and supplying air, and the air required for the growth of the seedlings cultured in the culture tank 105 from the outside and the culture tank 105 through the first air supply port 150 When the culture medium is detected by the bacteria and mold contamination by the contamination detection sensor 170 provided in the culture medium tank 125 and the second motor 220 to be supplied to the alarm sound to generate a warning sound Pollution warning device 230, a light quantity control device 240 for controlling the amount of light in accordance with the growth and photosynthesis of the seedlings, the first and second motors (210, 220), pollution warning device 230, light quantity control device ( Display with integrated control and 240 operating status Integrated control device (200) consisting of 260; It is configured inside the culture tank 105 to circulate the external air supplied from the first air supply unit 150 in the culture tank 105 to directly supply to the roots and stems of the seedlings to promote the growth of the seedlings. Ventilation control device 300 to be; And Located at the top of the culture vessel cover 110, according to the amount of light required for photosynthesis of the seedlings, the illumination control device for directly supplying the light controlled by the light amount control device 240 to the seedlings to promote the growth of the seedlings ( Culture apparatus for purifying seedlings growth, characterized in that it comprises a 400). According to claim 1, The ventilation amount control device 300, A second air supply port 305 installed inside the culture tank 105 and installed in series with the first air supply port 150, and an air supply unit 315 provided with a seating step 310 therein; The first porous tool 325 is composed of a material which is seated on the seating step 310 of the air supply unit 315 and is not corroded by the culture medium, and the air is discharged to the first porous tool 325. Absorbing agent 320 and the culture medium supplied to the air supply unit 315 is in close contact with the upper portion of the first porous plate 320 to maintain a constant amount of air discharged to the first porous tool 325 The second porous tool 340 is configured to be alternately stacked with the first surface fiber 330 and the first porous plate 320 so as to alternate with the first porous tool 325 of the first porous plate 320. The second porous plate 335 for discharging air to the second porous tool 340 and the culture medium supplied to the air supply unit 315 are absorbed to be in close contact with the upper portion of the second porous plate 335 to form a second porous tool ( The second surface fiber 345 to maintain a constant amount of air discharged to the 340 and the second porous plate 335 is laminated on the top of the air supply unit 315 and the first and second Opening (325 340) in the same manner as in forming the third tool 355. The third configuration is plantlets growth purification which comprises a perforated plate 350 of the culture device for that. According to claim 1, wherein the illumination control device 400, A light emitting unit 410 positioned above the culture vessel cover 110 to emit light controlled by the light quantity control device 240 according to the amount of light required for photosynthesis of the seedling plant; An optical panel 430 including a plurality of small fans 420 to remove heat generated from the light emitting unit 410; A lower support 440 configured to support a load of the optical panel 430; An elevation cylinder 450 inserted / formed into the lower support 440 and configured to penetrate the optical panel 430; An upper plate 460 provided at an upper end of the optical panel 430 and configured to be connected to the elevating cylinder 450; On one side of the upper plate 460 drive knob 470 for generating a driving force by rotation, and The lifting cylinder 450 and the optical panel 430 is elevated due to the driving of the drive handle 470, the culture apparatus for purifying seedling growth. The method of claim 3, wherein the lifting cylinder 450, A spiral lifting bar 480 which is formed of a spiral and is inserted / formed into the lower support 440 by engaging with a spiral formed in the lower support 440; The spiral lifting bar 480 and the optical panel 430 are elevated by the driving of the driving knob 470, the culture apparatus for purifying seedling growth.

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