KR100785057B1 - New breed of cymbidium, yang gwang - Google Patents

Abstract

A new Cymbidium Yang Gwang is provided to mass-provide the novel species Cymbidium Yang Gwang with improved ornamental value by having a pattern in a bud. A new Cymbidium Yang Gwang is prepared by explanting a growing point of a conventional Cymbidium 'Daeguk' in MS(Murashige-Skoog) medium to select and proliferate a bud-mutated protocorm-like body(PLB) 1, breeding shoot differentiated from the PLB, being fixed by subculturing more than 3 generation and being asexually reproduced by tissue-culturing and has the characteristics of: (a) having a yellow-white pattern at the center of a leaf and a green edge of the leaf; (b) having the leaf gloss; (c) having a leaf length of about 20 cm; (d) having a leaf width of 0.8-1.2cm; (e) having 4-5 leaves; (f) having a bud pattern of the inside stripe; (g) flowering period of March to May, and September to October; (h) two times per year of flowering; (i) a production method of tissue culture; and (j) being used for differentiation.

Description

New breed of Cymbidium, Yang Gwang

Figure 1 is a picture of the new symbidium 'yanggwang' holy grave (28 months after the purification) of the present invention.

Figure 2 is a photograph of the leaf pattern (medium pressure) of the morphological characteristics of the new Symbidium varieties of the present invention.

Figure 3 is a photograph taken 60 days after passage of the symbiotic meridium (mericlone) subcultured the present invention.

The present invention relates to a new breed of poultry symbidium 'sunlight' plants. More specifically, the present invention relates to a plant 'lighting' belonging to a new breed of poultry symbidium, which is fixed as an excellent new breed for differentiation as a medium-pressure that blooms twice a year.

About 70 species of Cymbidium are native to tropical Asia, ranging from the Himalayas in the north to Japan in the east and northern Australia in the south. It has long been recognized as an eruption in Korea, China, and Japan, and it is known as one of the four soldiers by the literary people. The temperate symbidium of native to East Asia is called Dongyang Ran, and the tropical or subtropical symbidium is called Western Ran.

On the other hand, the mass production of symbidium is possible by tissue culture techniques, in particular, by the nutrient system, that is, mercury technology, which is obtained by extracting, culturing and propagating growth points or parietal fission tissues. . Therefore, symbidium can be mass-produced by the mercury technology, and it is easy to cultivate new varieties, and as a result, it can satisfy the demand of consumers by the low price.

In addition, the symbidium varieties produced and sold in the past are 'Oriental', 'Big Country', 'Hanran', 'Chunran', 'Daemyung Bonded', 'Steel timber timber', 'Geumhwasan', 'Sun dust', 'Miss Taipai' 'There is. Symbidium, which has been produced and sold in the past, is difficult to sell because of its low ornamental value, and its adaptability and growth rate is low due to 100% import seedlings from China, Japan, and Taiwan. And, there is a problem that the area of the pattern is small.

Accordingly, the present inventors intended to invent a new kind of symbidium that can be sold all year round to secure ornamental objects other than flowers by increasing the number of flowers by increasing the number of flowers, and increasing the ornamental value by adding patterns to the buds.

Accordingly, an object of the present invention is to provide a large amount of new varieties of symbidium with a high ornamental value inexpensively.

The object of the present invention is to extract the growth point of the 'large' varieties of existing egg yolk symbidium and wound on the MS medium added hormones to select a system of azo-modified protoplasts (PLB) by subcultured after passage of tissue culture Achieved.

Symbidium new varieties of the present invention, 'lightning' is to extract the growth point of the 'large power' of existing egg symbidium and wound on an MS medium containing hormones to induce azo mutations, and then the azo mutant protoplasts (PLB) with a heavy pressure arc After selection and mass propagation, the induction and rooting of the plant are induced therefrom, and the cultured mercury is passaged over three generations to fix the variety.

The horticultural characteristics of the new symbidium varieties of the present invention are as follows.

1) leaf pattern: medium pressure

2) Gloss of Leaf: Yes

3) Length of leaves: around 20cm

4) leaf width: 0.8 ~ 1.2cm

5) Number of leaves: 4-5

6) Bud Pattern: Genus Stripes

7) Flowering season: March ~ May, September ~ October (2 times)

8) Blooming times: twice a year

9) Production method: tissue culture

10) Use of varieties: for differentiation

The present invention 'yanggwang' varieties are medium pressure lake, yellowish white pattern among the leaves, the leaf edge has a green abdominal ring. Among the leaf varieties, it is a cultivated medium-pressure lake species with yellowish white patterns in the leaves and green leaf rings at the edges of the leaves. Yellow stripe in the center of leaf and glossy, high ornamental value. The leaves are about 20cm long, the leaves are 0.8 ~ 1.2cm wide, and the leaves are 4 ~ 5 pieces.

Hereinafter, one specific example of the present invention will be described in detail, but the scope of the present invention is not limited thereto.

EXAMPLE

Example  One : Symbiotic  Extraction and growth of growth points in 'large country'

MS (Murashige-Skoog) extracting the growth point of egg symbidium 'large' in accordance with the method described in Korean Patent Application No. 2002-61313 filed by the applicant for azo variation of egg symbidium and adding hormones as shown in Table 1 It was wound in the medium.

Composition of MS (Murashige-Skoog) Medium Additive Amount NH ₄ NO ₃ 1650mg / L KNO ₃ 1900mg / L CaCl ₂ · 2H ₂ O 440 mg MgSO _{4 7} H ₂ O 370 mg KH ₂ PO ₄ 170mg H ₃ BO ₃ 6.2mg MnSO ₄ 4H ₂ O 22.3mg ZnSO ₄ · 7H ₂ O 8.6mg KI 0.83 mg Na ₂ MoO ₄ 2H ₂ O 0.25mg CuCl ₂ · 6H ₂ O 0.025mg CoCl ₂ · 6H ₂ O 0.025mg FeSO ₄ 7H ₂ O 27.8mg Na ₂ , EDTA, 2H ₂ O 37.3 mg sucrose 20mg inositol 80 mg nicotinic acid 0.5mg pyridoxineHCl 0.5mg thiamine-HCl 0.1mg glycine 2.0mg casein hydrolysate 1.0 g Agar 8 g NAA 0.6 ppm Kinetin 0.2ppm Coconut milk 30 cc pH 5.5

Example  2 : Mutated Protozoa ( PLB Selection and proliferation of 1 strain

After 4 months of growth point in the MS medium, one system of uniform and clear azomutosis protoplasts (PLB) was selected and mass-proliferated in MS medium as shown in Table 2.

MS medium for proliferating protoplasts Additive Amount NH ₄ NO ₃ 1650mg / L KNO ₃ 1900mg / L CaCl ₂ · 2H ₂ O 440 mg / L MgSO _{4 7} H ₂ O 370mg / L KH ₂ PO ₄ 170mg / L H ₃ BO ₃ 6.2mg / L MnSO ₄ 7H ₂ O 22.3mg / L ZnSO ₄ · 7H ₂ O 8.6mg / L KI 0.83 mg / L Na ₂ MoO ₄ 2H ₂ O 0.25mg / L CuSO ₄ · 5H ₂ O 0.025mg / L CoCl ₂ · 6H ₂ O 0.025mg / L FeSO ₄ 7H ₂ O 27.8mg / L Na ₂ , EDTA, 2H ₂ O 27.3 mg / L sucrose 20mg / L inositol 80mg / L nicotinic acid 0.5mg / L pyridoxineHCl 0.5mg / L thiamine, HCI 0.1mg / L glycine 2.0mg / L casein hydrolysate 1.0mg / L Agar 8g / L NAA 1.2 ppm Kinetin 0.2ppm BA 0.5 ppm Coconut milk 30 cc pH 5.5

Example  3: present invention Symbidium  Induction of new plant species, Rooting

remind Hyponex medium as shown in Table 3 was used for induction and rooting of new plant varieties of the present invention from mass produced protoplasts (PLB) in Example 2.

Hyponex medium for induction and rooting of new plant species of the present invention Additive Amount Hyponex 2.6 g / L peptone 2 g / L Manana 30 g / L Apple 10 g / L potato 20g / L Charcoal 0.2 g / L Agar 8g / L pH 5.5 Sucrose 30 g / L

On the other hand, when shoots were differentiated from PLBs, they were transplanted into 300mL flasks for seedlings, but seedlings were fixed based on 20 weeks in 300mL flasks and 40 weeks in 500mL flasks, and then cultured for three generations. In examining the morphological characteristics, the leaf length was measured as the average height of 90% or more leaves, the lateral width was the middle leaf width, and the number of leaves was the number of leaves. The horticultural characteristics of the new varieties 'lightning' of the present invention are shown in Table 4 below.

Comparison of the horticultural characteristics of the new varieties Symbidium 'lighting' of the present invention division Morphological features of the new symbidium varieties of the present invention Morphological features of the existing Symbidium varieties 'Big Country' Leaf pattern Heavy pressure Abdomen (yellow stripes on white background) Gloss of leaves has exist has exist Leaf length Around 20cm 40 cm Side width 0.8-1.2 cm 1.5-1.8 cm Number of leaves 4 to 5 4 to 5 Flower bud Genus stripes stripe Flowering time March to May, September to October (twice) March to May, September to October (twice) Blooming 2 times / year 2 times / year Production method 100% tissue culture 100% tissue culture Quality uniformity height height Varieties of use Eruption Eruption virus none none Disease resistance Class A Class A

Example  4: new breed of the present invention Symbidium  Purifying 'lights'

As described in Example 3, seedlings of about 15 cm that passed about three months after subculture were put into a flask, and the medium was removed by tweezers, and the medium was removed with distilled water. Purifying conditions were 10% of natural light, 5000 ~ 10000lux, atmospheric humidity is shaded and planted in a good air-permeable basket and 70-80% was maintained to avoid drying.

Cultivation temperature of the new varieties of symbidium 'yanggwang' of the present invention is suitable for 20 ~ 25 ℃ and very high adaptability to low temperature, it is advantageous to give water frequently enough to like water. Sunlight is good for shading 60-70% in summer and faster than other eggs. Therefore, Table 5 shows the management environment of the new varieties symbidium 'lightning' of the present invention.

Management environment of new varieties Symbidium 'lighting' of the present invention Spring (March-May and Below) Summer (from June to bottom of August) Autumn (from September to October) Winter (from November to March) Temperature Growth temperature (20 ~ 25 ℃) Around 10 ℃ Humidity Air Humidity (60 ~ 75%) 50% ventilation Good ventilation results in less pest and health Avoid cold winds Water cycle We give enough about once / 3-4 days 1/10/10 days sunshine 10-20% 60-70% 10% 0% Fertilizer Leaf fertilization (2 ~ 3 times / month), solid fertilizer is given in mid April Refrain Pest Fungicides, insecticides alternately once every 2-3 weeks 1 time / month breeding Dispensing (Dispensing with 4 ~ 5 Tips), Tissue Culture Split We carry out every 1-2 years in spring (March-April) Usage Eruption

As described above, the present invention relates to a new breed of poultry symbidium, the effect of providing a 'Pomp' plant belonging to a new breed of poultry symbidium for differentiation overwhelming other varieties with only one pattern of the polymerization lake forming the most expensive of the spring This is a very useful invention in the new plant breeding industry.

Claims (1)

After extracting the growth point from the 'large power' of existing egg symbidium, and incubating it with MS (Murashige-Skoog) medium, it selects and propagates 1 strains of uniform and clear azomutosis protoplasts (PLBs) in medium pressure arcs and then proliferates them. Cymbidium Yang Gwang, a new breed of poultry symbidium, characterized by cultivation of shoots differentiated from shoots and subcultured by more than three generations, has the following horticultural characteristics, and asexual reproduction by tissue culture. plant. 1) leaf pattern: medium pressure 2) Gloss of Leaf: Yes 3) Length of leaves: around 20cm 4) leaf width: 0.8 ~ 1.2cm 5) Number of leaves: 4-5 6) Bud Pattern: Genus Stripes 7) Flowering season: March ~ May, September ~ October (2 times) 8) Blooming time: twice a year 9) Production method: tissue culture 10) Use of varieties: for differentiation

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