KR100764003B1 - Lensless Optical Microscope-on-a-Chip and Lensless Imaging System Thereof - Google Patents

Abstract

The present invention relates to an optical microscope that does not use a lens and an image acquisition system using the same. In particular, it has a lensless structure by using a collimator that passes only light coming near the vertical in front of the digital image sensor. This structure is a set of side-by-side tubes, each with a small hole at the end of the specimen to be observed, to perform one-to-one projection from one point on the sample to one pixel on the image sensor. Since the lens that enlarges or reduces the image is excluded, the manufacturing cost of the microscope is reduced and miniaturization is possible.

Microscope on chip, optical microscope without lens, miniaturization, portability, interpolation, digital, fluorescence.

Description

Lensless Optical Microscope on Chip and Image Acquisition System Using It {Lensless Optical Microscope-on-a-Chip and Lensless Imaging System Thereof}             

1 is a structure of a microscope without a lens according to the present invention.

Figure 2 is a structure for passing only the light vertically entered in accordance with the present invention.

Figure 3 is a structure that passes only the light vertically entered for the dark field.

4 shows the internal structure of a lensless microscope system.

5 is an external configuration of a lensless microscope system.

6 is a functional configuration of a lensless microscope system.

7 shows various scan methods.

8 shows the interpolation principle (100 x 40 pixels)

FIG. 9 shows the interpolation principle (enlarge part of FIG. 8, 16 x 20 pixels)

FIG. 10 illustrates the interpolation principle (enlarged FIG. 9 and shows a grid)

<Explanation of symbols for main parts of the drawings>

10: A collimator that only passes light near the vertical

11: image sensor 12: sample

13: straight hole 14: barrier

L: Height of the structure D: Distance between the sample and the structure

P: hole spacing G: width of open hole

S: length of the sample at which light reaches a specific point in the pixel

The present invention relates to a compact microscope that is easy to carry and an image acquisition system using the same. In particular, an optical microscope and a lens on a lensless chip having a lensless structure using a collimator for passing only light vertically in front of a digital image sensor are provided. It relates to an image acquisition system used.

An optical microscope is a device that magnifies or observes a minute portion of an object by using light. It is an essential experimental instrument that is used for a variety of purposes, including biology, various scientific fields, such as semiconductor, medicine, and material science, as well as advanced education in schools. The structure of a general microscope uses an objective lens and an alternative lens, and usually has a magnification of 50X to 1000X. In the case of the human eye, first, the image of the sample is magnified with an objective lens to make a 20mm medium image, and then, using an alternative lens, a virtual image of 200mm size is placed 250mm away from the eye to make it visible. . Alternative lenses are usually 10X magnification, and objective lenses have 5X, 10X, 20X, 50X, 100X, and the like.

The ability to see the structure of a sample in detail is called resolution (or resolution). This is a factor that determines the performance of the microscope and is determined by the performance of the objective lens. Therefore, even if the magnification is increased by the combination of the objective lens and the eyepiece, if the performance of the objective lens is poor, the blurry image is simply enlarged, and the fine structure is not identified. In general, the total magnification of the microscope should be 500 to 1,000 times the numerical aperture (NA) of the objective lens. The resolution is expressed by the minimum distance d of the two points identified thereon, where d = λ / a, where the wavelength of the illumination light is λ and the numerical aperture of the objective lens is a. The wavelength of visible light is 400 nm to 700 nm, the numerical aperture of the dry objective lens is 0.05 to 0.95, and the liquid objective lens also has the highest 1.4, so the resolution of a microscope is usually about 0.4 μm.

Recently, due to the development of computer technology, digitalization of image information obtained from a microscope has been performed. In order to accept the current digital image, the structure of the optical microscope is changed to obtain an image. The camera uses the camera to reduce the image magnified by the objective of the optical microscope. The reason is that unlike the human eye, which is suitable for the 200mm image of 250mm distance, the digital image sensor is about 5 X 4mm. This is because it has a surface area. In other words, instead of using an alternative lens having a 10X magnification function, the intermediate image having a size of 20 mm is reduced by using the optical system of the camera to form an image on the sensor surface.

Digital image sensors have evolved rapidly over the last decade with the development of semiconductor technology. Semiconductor image sensors are classified into two types: charge coupled devices (CCD) and CMOS image sensors (CIS). CCD has been used for a relatively long time, including broadcast cameras, and CIS has been used in mobile phones and digital cameras since the late 2000s because of technology developed in the 90s. At present, the pixel size is about 4x4μm, and in the case of 1.3 million pixels having 1,300 horizontal and 1000 rectangular arrays, the sensor size is about 5x4mm.

Miniaturized imaging systems, on the other hand, are mainly studied based on confocal methods. The confocal method is a special type of microscope that accurately observes the shape of a specific thin layer in a thick sample. It is quite different from a general optical microscope such as using a laser and a pinhole. Recently, several organizations, including a team at the University of Arizona, have reported confocal microscope-on-a-chip (US 6749346, 2004; IEEE Jour. Of Quantum Electronics, 38 ( 2): 122-130, 2002; Optical Letters 29 (7): 706-708, 2004).

In addition to digital cameras, there are scanners that accept digital images. Except when only one axis of information such as a barcode scanner needs to be recognized, the scanner scans a document or sensor on one axis to process a two-dimensional image such as a document. In the case of general office use, the resolution of the scanner is 600 dpi, 1200 dpi, 2400 dpi, etc., and in the case of a film scanner, 4000 dpi, 8000 dpi is used. In each case, the pixel size is calculated from 10 to 42 μm for office and 3 to 6 μm for film. Digital cameras and scanners commonly process two-dimensional images and also have color (color) functions. The main differences are the structure of the image sensor and the presence or absence of lenses. Digital cameras use a two-dimensional sensor array to capture an image of a scene at a moment and use a lens, while a scanner uses and scans a one-dimensional sensor array—most often without a lens.

However, in the process of obtaining a digital image, a conventional microscope needs to magnify an image using an objective lens and then reduce it again using a camera. Therefore, there is a disadvantage in that the lens is used in this process, which is large in size and expensive, and also has a limitation in resolution due to optical aberration.

The present invention has been proposed to solve the problems of the prior art, and an object of the present invention is to obtain a digital image directly without enlarging / reducing an image using a lens, unlike a microscope using a conventional lens. If you use a collimator that passes only light vertically in front of the digital image sensor, you can obtain an image without a lens on the principle similar to a scanner. This unnecessary process can be eliminated, which can greatly contribute to the miniaturization of the microscope system. Also, the use of a lens has a limitation of resolution due to the numerical aperture (or optical aberration). Optical microscope on a lensless chip with advantages and images using the same It is to provide an acquisition system.

In order to achieve the object of the present invention, the present invention is an image sensor 11 and a plurality of straight holes of a rectangular shape made of a material that does not transmit light at a position corresponding to each pixel of the image sensor 11 ( 13) and comprises a collimator 10 that passes only light that is close to vertical, which performs one-to-one mapping with one pixel on the image sensor at a point on the sample. A microscope on a chip without a lens is provided.

In addition, the optical microscope on the chip without a dark field lens is a collimator (10) to perform one-to-one projection to one pixel on the image sensor 11, the blocking film 14 on the image sensor side for the dark field image It characterized in that it further comprises.

The microscope includes a slide glass 42 on which a sample sample is placed; Sample sample tray 53; A collimator 43, which is a structure that only passes light that enters near to the vertical; An image sensor 44 for processing an image using a CMOS image sensor (CIS) or a charge coupled device (CCD); A motor 66 capable of moving up, down, left and right; LCD display backlight can be applied, the light source 64 using a variety of solid light sources using a laser diode (LD), LED, organic EL, etc .; A controller (65) for controlling the motor (66) and the light source (64) which are movable up, down, left and right; A CPU 61 for processing digital images such as color correction and noise reduction and controlling all functions of the microscope system; A memory 62 for storing digital image information photographed from the image sensor 44; A communication unit 68 for the microscope system to transmit images to and send commands to other devices such as PCs, TVs, and printers using wired or wireless digital communication; An antenna 69 for the communication unit 68 to wirelessly use a mobile phone technology (W-CDMA, CDMA-2000), wireless LAN, Bluetooth, IR / RF transmission method; A connection jack (55) connected to the communication unit (68) outside the system for transmitting a digital picture by wire by USB, IEEE 1394, NTSC (TV video standard) transmission method; A user interface unit 67 for displaying a video to a user and providing a function of receiving a command; Operation keys 54 for inputting various operations such as zooming in / out, moving up, down, left, and right, and screen brighter / darker; A battery 46 for supplying operating power for the entire system; And an image taken now or already taken is provided with an integrated LCD display 52 for displaying to the user through appropriate processing.

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings. However, the following examples are merely to illustrate the present invention is not limited to the contents of the present invention.

The present invention implements a lensless image acquisition system, that is, a microscope, for observing microstructures, and implements a microscope with a memory, a display, and the like, and uses a one-to-one projection with each pixel of an image sensor at a point on a sample without a lens Microscopic images are obtained and darkfield images and fluorescence images can be obtained.

The microscope on a chip according to the present invention is composed of a collimator that passes only light that is perpendicular to the digital image sensor as shown in FIG. 1. The collimator performs one-to-one mapping with one pixel on the image sensor at a point on the sample, and the electrical results of the image sensor give the same information as the image seen by the microscope.

One-to-one projection without a lens is implemented as follows. That is, in the process of the light from the sample entering the image sensor, only the light coming near the vertical of the light from the sample is passed through the structure. This removes light scattered in different directions from the sample, and the amount of light entering the image sensor reflects the extent to which each part of the sample passes the light.

Next, the microscope (a) seen by a human eye, the microscope (b) using a digital camera, and the microscope (c) without a lens which this invention proposes are compared.

When observing with the human eye, first magnify the image of the sample with an objective lens to make a 20mm intermediate image, and then use an alternative lens to make a virtual image of 200mm size 250mm away from the eye and make it visible. . In the case of using a digital camera, the image magnified by the optical microscope objective lens is reduced again by using the camera, because the digital image sensor has a sensor surface area of about 5 x 4 mm. In other words, instead of using an alternative lens having a 10X magnification function, it is reduced by using an optical system of the camera (about 1 / 4X) to form an image on the sensor surface. The lensless microscope proposed by the present invention directly obtains an image using a structure that removes only scattered light. Since no lens is used, a small and light microscope can be produced. In addition, there are no numerical aperture (NA) problems caused by the lens, which will further improve the resolution.

Example 1 Compact Microscope without Lens to Obtain Bright Field Image

One way to make the collimator 10 which only passes light close to the vertical is to have several straight holes 13 with a material that does not transmit light. In other words, it is necessary to make a filter that absorbs scattered light and passes only light traveling in one direction, that is, a narrow pass angle. Looking at the structure, as shown in Figure 1 has a hole in the position corresponding to each pixel of the image sensor (11). The number of pixels and the number of holes are not exactly the same number. When obtaining a black and white image, it is good to have one hole for each pixel, and when obtaining a color image, it is good to arrange one hole in a total of four pixels, each of two horizontally and vertically. Since most image sensors are arranged in a rectangular shape, the hole arrangement is also rectangular. The figure shows a rectangular shape as an example of the shape of the hole, and the shape of the hole may be another shape. In one embodiment of the present invention to manufacture a filter in the form as shown in FIG. There are a variety of micromachining techniques (Micromachining, MEMS). Hot embossing, injection molding, replica molding, or the like can be used as a method for producing a microstructure using plastic as a material (Anal. Chem. 37; 550-575, 1998).

2 illustrates a light transmission path. The height L of the structure and the width G of the open hole are determined in consideration of the distance D between the sample and the structure and the hole spacing P. At each corresponding point of the sample 12, light enters only each pixel of the sensor, and the height L of the structure and the width G of the open hole are determined so as not to affect neighboring pixels. The hole spacing P is determined in relation to the pixel size of the image sensor as described above, which is usually one or two times the pixel size. In one embodiment of the invention, the distance D between the sample and the structure will be the thickness of the slide glass. The height (L) of the structure is to be twice the distance (D) between the sample and the structure, the width (G) of the open hole is to be 1/3 of the hole spacing (P). In general, if the slide is 1 mm thick, the height of the structure is 2 mm, and if the size of the image sensor is 4 μm and the hole spacing is twice that, the width of the open hole is about 2.7 μm. The length S on the sample at which light reaches a specific point in the pixel is determined by the height L of the structure and the width G of the open hole. Assuming that the light goes straight, it is expressed as the following equation.

In the case where L = 2D and G = P / 3, the length S on the sample is P / 2.

Example 2 Compact Microscope without Lens to Obtain Dark Field Image

Different structures are used for darkfield imaging. Dark field imaging is a way to observe colorless specimens, such as bacteria and living cells, by illuminating the object from the side and viewing it in the darkest background possible. These objects are difficult to observe because they absorb little light in the bright field. The human eye is recognizable when the local intensity change is more than 10-20%, so in the bright field many samples do not modulate this much light. However, when the dark field method is used, the background area is black because no light is input, and only the sample part is lighted so that it can be easily seen.

3 is a collimator structure for obtaining darkfield images. In the filter structure of FIG. 2, a light blocking layer 14 is added to the lower portion. This serves to block only light that is exactly vertical, ie light that passes through the sample. Light scattered slightly from the sample 12 passes through the filter at a slightly oblique angle and reaches the image sensor 11. Of course, the light directed to the neighboring pixels by breaking a lot is blocked. The width of the blocking film 14 and the distance from the image sensor 11 are determined in consideration of the amount of light to pass through. In one embodiment of the present invention, the blocking film 14 is directly in contact with the image sensor 11, the width of which is equal to the width G of the open hole.

In the dark field method, some of the light bent from the sample is blocked by the blocking film 14, so the amount of light entering the image sensor 11 is reduced. The length S on the sample at which light reaches a specific point on the pixel does not differ from that of the bright field, but the area on the image sensor 11 that receives light is reduced. When the blocking film 14 is in direct contact with the image sensor 11 and its width is equal to the width of the open hole, the ratio of the reduced light is as follows.

When G = P / 3, the reduced rate is 1/9, and light from the sample passes through 8/9 of the bright field.

Example 3 Compact Microscope without Lens for Fluorescence Measurement

The present invention may also enable fluorescence measurement. Fluorescence may be measured using an excitation filter for a light source and an emission filter for an image sensor. Excitation filter (Excitation filter) can be implemented by sandwiching a thin film between the light source and the sample. Alternatively, it may be deposited during the image sensor fabrication process. It is also possible to use a laser light source that emits light of a specific wavelength.

An emission filter can also be produced by sandwiching a thin film between a light source and a sample and a thin film deposition method. If you make a filter that passes different wavelengths for each pixel during film deposition, you can observe multiple wavelengths of fluorescence on one sensor, just as you would for a color image. Alternatively, it is possible to use an existing natural color filter instead of an emission filter, and then to deconvolution each pixel in the wavelength range of light to track which wavelength of light has passed. .

Example 4 Fabrication of a Lensless Microscope System

The lensless microscopy system according to the invention integrates several elements together with a filter and an image sensor. 4 and 5 schematically show the internal structure and external shape of a small microscope without a lens.

Referring to FIG. 4, the internal structure of the small microscope without a lens includes a light source 41, a slide glass 42 on which a sample sample is raised, a collimator 43, which is a structure that passes only light coming close to the vertical, and an image sensor. 44, an electronic circuit board 45, a battery 46 and an electronic circuit chip 47.

Referring to FIG. 5, the external shape of the lensless microscope is a sample of the LCD display 52 for displaying data on the small microscope body 51 and a sample sample detachable to an opening of a predetermined size on one side of the small microscope body. USB, IEEE1394, NTSC (TV video standard) transmission of digital photos taken by the image sensor on the tray 53, operation keys 54 for up, down, left and right movement, brightness adjustment, temperature control and one side of the upper right External connection jack 55 for wired transmission in a manner.

6 is a diagram in which each device is connected by function. Representative functional elements include a slide glass 42, a sample sample tray 53, a collimator 43, an image sensor 44, a CPU 61, and a memory 62, which are structures that allow only light entering near to the vertical to pass through. ), A battery 46, a light source 64, a motor 66, a controller 65, a user interface 67, an operation key 54, an LCD display 52, and the like.

The CPU 61 processes digital images such as color correction and noise removal and controls all functions of the microscope system. The memory 62 is a storage for storing digital image information photographed from the image sensor 44, and uses a combination of several memories.

The memory 62 requires a nonvolatile FRAM for storing an image even when the power is turned off, and a fast SRAM for image processing. The image sensor 44 uses a CMOS image sensor (CIS) or a charge coupled device (CCD).

The communication unit 68 allows the microscope system to transmit images and send and receive commands to other devices such as a PC, a TV, or a printer by using wired or wireless digital communication. In the case of wireless, the antenna 69 is used, and a transmission method may use mobile phone technology (W-CDMA, CDMA-2000), wireless LAN, Bluetooth, IR / RF, and the like. It is connected to the communication unit 68 by a wire and should have a connection jack 55 outside the system, the transmission method may be applied to USB, IEEE 1394, NTSC (TV video standard) and the like.

The user interface 67 has a function of showing an image to a user and receiving a command. The image taken now or already taken can be checked by the user through the LCD display 52 through appropriate processing. Various operations such as enlargement / reduction, up / down, left / right movement, lighter / darker the screen are performed by using the operation key 54. The controller 65 has a function of controlling the motor 66 and the light source 64 that can be moved up, down, left, and right. Various solid light sources may be used as the light source 64. An LCD display backlight may be applied, and a laser diode (LD), a light emitting diode (LED), and an organic EL may be used. The battery 46 supplies the operating power of the entire system.

Example 5 Image Acquisition Method Using a Lensless Microscope System

Using the Scan function, you get an image larger than the sensor size. When the sample and the sensor are moved relative to each other in two horizontal and vertical axes, an image of another part can be obtained and a sample over a large area can be measured. This method, combined with artificial intelligence, can be used to find and photograph small samples (10 μm) in large spaces (10 x 10 mm). As a method of horizontally and vertically moving, there are various methods as shown in FIG. 7. The filter and the image sensor are fixed, the method of moving only the specimen (sample) horizontally and vertically (71), the sample and the filter are fixed, the method of moving the image sensor horizontally and vertically (72), the filter is fixed, the sample And a method 73 in which the image sensor and the image sensor move in two axes perpendicular to each other.

By applying the sub-pixel method, the image resolution of a small microscope can be improved. The image is taken several times at slightly different locations, which are then image processed to obtain a finer image than the original pixel pitch. As shown in FIG. 8, the image 81 is moved horizontally and vertically by 1/2 pixel to obtain four photographs 82-85, and this is processed to obtain an image 86 having four times the resolution (number of pixels in the image) than the obtained photograph. 9 is an enlarged image 91-96 of the letter K in each picture 81-86 of FIG. 8. FIG. 10 is a diagram 101-105 showing some of FIG. 9 with the pixel grid. Photos 101, 104, and 105 are enlarged images 91, 95, and 96, respectively, and 102 and 103 represent grids of 101, respectively, resulting in 104 and 105 quantization. (You can also say that the virtual pixel size has been reduced to 1/4 in area and 1/2 in length because the number of pixels has increased four times in the same area.) It is also possible to increase the resolution by taking more pictures. In Figs. 7 to 10, four shots were taken by moving 1/2 to increase the number of pixels four times in the same area. Similarly, you can take a total of nine pictures by moving one third of the pixel size, or move one quarter of the pixel size. As a method of moving vertically and horizontally, there are various methods as in the previous scanning function (FIG. 7). The filter and the image sensor are fixed, the sample moves (71), the sample and the filter are fixed, the image sensor moves (72), the filter is fixed, and the sample and the image sensor move in two axes perpendicular to each other. 73, and the like.

The present invention may be more useful if implemented by adding the following additional functions.

1) Used to measure bio microarrays.

A small lensless microscope is used to measure DNA microarrays and protein microarrays.

2) It has a temperature control function.

In the case of cells, they react with temperature.

3) It has a function to put a specific substance.

It is more useful to add the function of putting the specific substance in the microscope according to the present invention. For example, a dye may be added to the specific material to dye inside the microscope. In addition, if the specific substance has a function of adding a biochemical material-a biochemical material such as a new drug candidate can be put in the microscope and tested. In addition, the specific substance has a function of changing or adjusting the buffer composition (buffer) affecting the cells and chemical reactions. This function is, in engineering terms, to add a liquid substance, so a micropump can be used. Currently, micro pumps are being developed with various MEMS technologies. Alternatively, microfluidics may be used instead of pumps by electrophoretic force, capillary force, electro-wetting, or laminar flow.

As described above, although described with reference to a preferred embodiment of the present invention, those skilled in the art various modifications of the present invention without departing from the spirit and scope of the invention described in the claims below Or it may be modified.

 As described above, the optical microscope on the lensless chip and the image acquisition system using the same according to the present invention provide a lensless image acquisition system for observing microstructures by miniaturizing a memory, an LCD display, and a microscope without using a lens. Implement under a microscope. Conventional microscopes have to magnify an image using an objective lens and then reduce it again using a camera in the process of obtaining a digital image. Therefore, there is a disadvantage in that the lens is used in this process, which is large in size and expensive, and also has a limitation in resolution due to optical aberration. The present invention focuses on the fact that the object size of a microscope and the size of an image sensor are similar, and is characterized in that a lens is not used for enlargement and reduction. The microscope system will be similar to a cell phone or digital camera, which is easy to carry and saves a lot of money. By using the present invention, the microscope can be miniaturized and has advantages such as cost reduction and portability. In addition, the microscope on the chip without the lens and the microscope system on the chip according to the present invention, it is easy to add a number of additional functions, such as dye or drug injection, which was difficult to implement in the conventional microscope, it will be able to increase the usability of the microscope system.

Claims (23)

One-to-one mapping with an image sensor and multiple tubes formed of a material that does not transmit light at a location corresponding to each pixel of the image sensor and one pixel on the image sensor at each point on the sample Includes a collimator for passing only light that comes close to the vertical for each pixel, And the collimator further comprises a barrier on the image sensor side for darkfield imaging. 2. The collimator of claim 1, wherein a collimator that performs one-to-one projection to each pixel on the image sensor is a set of side-by-side tubes, each having a small hole at the end of the specimen to be observed. Optical microscope. delete The optical microscope of claim 1, wherein the image sensor is equipped with one or two or more filters for passing only light of a specific wavelength band to obtain a fluorescent image. The optical microscope on a lensless chip of claim 1, wherein the image sensor uses a CMOS image sensor (CIS) or a charge coupled device (CCD). The method of claim 1, wherein the microscope A slide glass 42 on which a sample sample is raised; Sample sample tray 53; A collimator 43, which is a structure that only passes light that enters near to the vertical; An image sensor 44 for processing an image using a CMOS image sensor (CIS) or a charge coupled device (CCD); A motor 66 capable of moving up, down, left and right; LCD display backlight can be applied, the light source 64 using a variety of solid light sources using a laser diode (LD), LED, organic EL, etc .; A controller (65) for controlling the motor (66) and the light source (64) which are movable up, down, left and right; A CPU 61 for processing digital images such as color correction and noise reduction and controlling all functions of the microscope system; A memory 62 for storing digital image information photographed from the image sensor 44; A communication unit 68 for the microscope system to transmit images to and send commands to other devices such as PCs, TVs, and printers using wired or wireless digital communication; An antenna 69 for the communication unit 68 to wirelessly use a mobile phone technology (W-CDMA, CDMA-2000), wireless LAN, Bluetooth, IR / RF transmission method; A connection jack (55) connected to the communication unit (68) outside the system for transmitting a digital picture by wire by USB, IEEE 1394, NTSC (TV video standard) transmission method; A user interface unit 67 for displaying a video to a user and providing a function of receiving a command; Operation keys 54 for inputting various operations such as zooming in / out, moving up, down, left, and right, and screen brighter / darker; A battery 46 for supplying operating power for the entire system; And Image taken now or already taken is an optical microscope on a lensless chip, characterized in that the LCD display 52 which is directly displayed to the user through an appropriate process. The optical microscope of claim 1, wherein the microscope has a horizontal scanning function, and in implementing the moving function, the image sample moves on the x-axis and the y-axis. 8. The optical microscope according to claim 7, wherein in implementing the movement function, the direction in which the sample and the image sensor move with each other moves in one direction so as to be perpendicular to each other. 7. A lensless chipped optical microscope according to claim 6, wherein a solid light source element is used as said light source. 7. The lensless chipped optical microscope according to claim 6, wherein a light emitting diode (LD) and a light emitting diode (LD) are used as the light source. 7. The lensless chipped optical microscope according to claim 6, wherein an organic electroluminescence (EL) element is used as the light source. 7. The lensless chipped optical microscope of claim 6, wherein digital photographs can be transferred to other devices, including PCs and TVs. 13. The lensless chip optical microscope of claim 12, wherein the digital photograph is transmitted by USB, IEEE1394, or NTSC (TV video standard). The method of claim 12, wherein the digital photo is transmitted wirelessly using any one of cellular phone technology (W-CDMA, CDMA-2000), wireless LAN, Bluetooth, and IR / RF. Optical microscope on chip without lens. 7. The lensless chipped optical microscope of claim 6, wherein all of the functions of the microscope are operable externally through a wired or wireless network. In an image acquisition method using a microscope on a lensless chip comprising an image sensor and a collimator that performs one-to-one projection to one pixel on the image sensor at a point on a sample, Lens acquisition method using a microscope on the chip without a lens to take an image several times at a slightly different position, using the sub-pixel method to obtain a finer resolution than the original pixel size by processing the image Image acquisition method using on-chip microscopy. The method of claim 16, An image acquisition method using a microscope on a lensless chip is obtained by using a microscope on the chip without a lens to obtain four pictures by moving 1/2 pixel horizontally and vertically, and processing this to increase the resolution four times. . delete 7. The lensless chipped optical microscope according to claim 6, wherein the lensless chipped optical microscope has a temperature control function. 7. The lensless chipped optical microscope of claim 6, wherein the optical microscope on the chipless lens has a function of inserting a specific substance and a function of introducing a dye into the specific substance. 21. An optical microscope on a lensless chip as set forth in claim 20, wherein biochemical materials such as candidates for drug development are introduced into the specific materials. 21. An optical lens-on-chip microscopy as recited in claim 20, wherein said specific substance has a function of altering buffer composition that affects cellular and chemical reactions. 21. A lensless chipped optical microscope as recited in claim 20, wherein micropump or microfluidic technology is used for the injection of the particular material.

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