KR100727369B1 - Preparation of health functional food using woolgum, doonglerae, and maltodextrine - Google Patents

Abstract

A functional health food composition containing Curcumae longae Radix, Polygonatum odoratum var. pluriflorum and maltodextrin is provided. The food composition is useful for improving liver function and formulated into liquid or encapsulated powder and has improved palatability. The functional health food composition is prepared by mixing Curcumae longae Radix, Polygonatum odoratum var. pluriflorum and maltodextrin in a weight ratio of 1:10:20, agitating with a plate agitator, drying with a plate dryer, homogenizing with a homogenizer and grinding with a grinder. For an example, 50ml Curcumae longae Radix is mixed with 500ml Polygonatum odoratum var. pluriflorum extract and 500g maltodextrin, agitated with a plate agitator of 180±30deg.C for 15min, dried with a plate dryer of 130±30deg.C for 30min, homogenized with a homogenizer for 5min, ground and formed into a hard gelatin capsule.

Description

Preparation of Health Functional Food Using Woolgum, Doonglerae, and Maltodextrine}

1-A graph of a calibration curve in which the curcumin standard solution is diluted to 1.6 to 12.8 ppm in the present invention.

The present invention relates to a health functional food composition using turmeric, round gourd and maltodextrin, and more particularly, to a food composition capable of providing a health functional food having an effect of treating and protecting the liver and having improved functionality and palatability.

Modern people are exposed to various chronic degenerative diseases, also known as adult diseases, are shorter than in the past, and are exposed to many health risks due to environmental pollution, increased intake of ready-to-eat foods, and mental and physical stress. Recently, in the field of medicine, the medical field is devoted to the development of new drugs to improve the health of human beings, and efforts are being made to find materials that can control various intractable diseases from natural materials.

However, various conventional food additives, etc. are used to interfere with the normal functioning of human organs by the abuse of chemical drugs, thereby having a lung end that rather harms health.

On the other hand, natural substances obtained from nature are easily absorbed by our bodies without rejection compared with chemicals to promote health functionalities and have fewer side effects than chemicals, and thus the pharmacological utility of these natural substances is greatly evaluated. It is more urgent than ever.

Curcuma longa (L) is composed of 1 ~ 3% of yellow cow, which is the main ingredient of curcumin, turmeron, dehydroturmeron, gingiberen, dialphapelandrene, cineol, alpha sabinel and borneole It is composed of 1 ~ 5% of essential oil, 2.4% of nonvolatile oil, 50% of starch, 5% of crude fiber, 4% of ash, and 16% of moisture.The pharmacological action is the effect of curcumin-like substances and essential oils. By the effect of biliary tract, hepatitis treatment, gastric juice secretion, diuretic, detoxification, anti-cancer, galactose treatment, as well as reducing urobiline urine, hepatocellular regeneration promoting effect, strong antibacterial effect It is known.

Also, Polygonatum odoratum var.pluriflorum is a perennial herb of the monocotyledonous plant, Liliaceae, and edible young leaves and root stems. In oriental medicine, root stems are alternately used to treat diabetes, heart failure, etc. In addition, if you drink it as a tea, you can improve the palatability by forming a delicious flavor.The root is rich in sugar, vitamins, minerals, etc., and contains saponin that sedates the central nervous system, strengthening the heart and nourishing tonic. It is known to have good effects on anti-aging, weakness and fatigue after illness, and skin care.

Maltodextrin is a carbohydrate that has a degree of polymerization up to maltose produced by hydrolysis of starch.It is known as a raw material of carbohydrate supplements that absorbs blood faster than glucose and fructose but facilitates blood sugar reaction. It is used as an excipient for combinations and drugs.

Curcumin, in particular, is widely known as an anticancer substance at the National Cancer Institute (NCI) in the United States.Texas State University researchers in Houston, USA, killed tumor cells containing curcumin in turmeric and cloned tumor cells. Experimental results for blocking complex myeloma cancer cells caused by the disease have been published. In Korea, it is possible to inhibit angiogenesis, which is known to cause intractable diseases such as cancer, diabetes, psoriasis, rheumatism and dementia. It is reported as a bioactive substance.

However, even though turmeric, dongle and maltodextrin are very desirable ingredients for the production of natural functional foods, no health functional food has been developed using the turmeric, dongle and maltodextrin components and extracts thereof.

It is believed that this is due to the strong irritant odor, which is peculiar to turmeric, and even though a small amount of turmeric is consumed, the flavor is reduced by the strong irritant odor, and the palatability of the consumer is reduced due to the deterioration of the aesthetics of the appearance. ) Has a significantly reduced lung.

Therefore, the present invention prevents consumers' texture from being degraded by offsetting the strong irritant odor of conventional turmeric by the dongle and maltodextrin, and enhances consumer's palatability for the functional food composed of the turmeric, dongle and maltodextrin mixed composition. In addition to improving the appearance, odor and flavor, and providing healthy functional food composition using turmeric, round goose and maltodextrin which can improve liver function, it greatly improves national health as well as economic added value of production and processors. Its purpose is to contribute.

Health functional food composition using turmeric, roundley and maltodextrin for achieving the object of the present invention as described above are as follows.

The present inventors have been conducting a study for preparing a concentration-specific (w / v) turmeric, a coniferous and maltodextrin mixed composition for a long time to prepare a health functional food containing turmeric, and also a mixture of the turmeric, coniferous and maltodextrin by the concentration Was prepared with a liquid and capsules, and experiments were conducted for a long time to examine the effects of their administration.

As a result, it was possible to obtain a turmeric, roundley and maltodextrin mixed composition for the health functional food based on quantitative experimental data, wherein the most preferable blending ratio of the turmeric, roundley and maltodextrin mixed composition is turmeric 1: roundley 10: malto Dextrin 10.

The turmeric extract was prepared by adding ethanol to turmeric powder and concentrating the filtrate obtained after shaking with a vacuum concentrator. The extract was added with water to the domestic dry mushroom and then used as a concentrate obtained after heating, and maltodextrin solution. Silver ovarian maltodextrin (Fine Fiber # 100489, Mitsuda Chemical Co., Ltd., Japan) was used.

In the present invention, the analysis of liver function is performed by analysis of AST (GOT), ALT (GPT), Total-bilirubin, albumin (AL), Total protein (TP), alkaline phosphatase (ALP) and liver damage to carbon tetrachloride. In order to analyze the functionalities of livers damaged by carbon tetrachloride, microscopic observations were performed. The animals were divided into preliminary experiments and the present experiments. Hepatic pre-treatment was tested. At this time, as a toxic substance for causing liver damage to the rats, a solution of 1: 1 mixed of olive oil with carbon tetrachloride (CCl ₄ ) was intraperitoneally injected before and after administration of a functional substance, and then treated with liver for treatment and protection. Was analyzed.

In the present invention, the sensory evaluation of appearance, flavor and odor was carried out on a 9 point hedonic scale.

As a result, it was confirmed that consumer palatability for the appearance, smell, and flavor of the turmeric, dongle, and maltodextrin mixed compositions of the present invention was remarkably enhanced, and an effective treatment and protective effect on liver damage could be confirmed experimentally.

The present invention will be described in more detail with reference to the following Examples.

Example 1

The turmeric used in this experiment was purchased by using Jindo acid turmeric powder purchased from Wellness Health Keeper. 400 ml of ethanol (GRAS grade, 95% purity) was added to 100 g of turmeric powder in a 1 L Erlenmeyer flask and agitator (Model 0750, Stirring). Stirring speed was 171 r.p.m. It left still for 30 minutes after stirring at room temperature for 3 hours. Thereafter, 1,200 ml of supernatant was recovered from 300 ml of each 1 L Erlenmeyer flask, and then concentrated under reduced pressure at 65 ° C. for 1 hour using a vacuum distillation unit (Model, Buchi 461 rotary vaccum evaporator, Switzerland). 100 ml of concentrate was prepared for use as a liquid.

Curcumin content of the functional substance present in the turmeric extract was quantified by the following HPLC analysis conditions using curcumin standards (purity 98%, Mw 368.38, Across Organics, USA), which are shown in Table 1 below. .

[ Table 1 ] Analytical Condition of Curcumin by HPLC

  Instrument Agilent  1100 series   Column  Nova-pak C18 , 3.9 × 300mm   Detector  UV detector, 424nm Mobile phase  Methanol 100%   Flow rate  1.0ml / min

The curcumin standard solution was diluted to 1.6 ~ 12.8ppm concentration, and then a calibration curve was prepared and shown in Table 2, and the calibration curve is shown in FIG.

The curcumin content of the turmeric sample was quantified by converting the dilution capacity for the turmeric sample from the concentration of the sample solution in the standard curve.

                                        Dilution capacity

Sample concentration = sample solution concentration in the standard curve × ----------

                                        Sample weight

                            20

Average Concentration = 8.8507 ppm × -------- = 0.3540 ppm

                           500 g

[ Table 2 ] Curcumin Creation of calibration curve

Curcumin  con. (X) Area of Curcumin (Y) 0.0000 0.000 1.6000 36.284 3.2000 72.179 6.4000 161.171 12.8000 373.308 Coeff . corr 0.996925 x = y-b / a a 29.4213 Y = a x + b b -12.6339 b = y-x / a

The 100 ml turmeric extract prepared by the above method was stored in a -18 ° C. freezer until it was used as a functional material, and then the sample was prepared as follows to check whether the obtained turmeric liquid extract could be used as a health functional substance. Prepared.

Sample 1: solution of turmeric liquid extract 5 g turbidly in 100 ml of distilled water

Sample 2: A solution of 0.5 g turmeric liquid extract in 100 ml of distilled water.

Sample 3: A solution of 0.1 g of turmeric liquid extract in 100 ml of distilled water.

Rats to be tested were purchased Sprague-Dawley (SD) male rats (250 ± 10g body weight), and after 7 days of pure breeding in the experimental animal room, the general symptoms were observed during the breeding period. Healthy animals that were absent were published in the test. Sprague-Dawley (SD) rats were selected as control and treatment groups, and randomly placed 5 rats per group. Purification room temperature was 22 ℃ (5 ℃ temperature difference), humidity 55 ℃ (5 ℃ difference), illuminance was maintained at 150-200Lux and brightness was changed every 12 hours based on 7am and 7pm. Animals were housed in two separate polycarbonate cages (260WX420LX180H mm, Daejong Instruments) for rats. For feed, rats were given solid feeds (orients) and drinking water was freely available for tap water.

First, we analyzed Sprague-Dawley male rats (weight 250) to analyze the effect of liver protection on the enzymatic activity of AST (GOT) and ALT (GPT) in serum of rats that caused liver damage with carbon tetrachloride (CCl ₄ ). ± 10 g) were placed in groups of 5, the first group: the normal group, the second group is the control group, the third group is the composition 40 mg / kg administration group prepared in Example 1, the fourth group is in Example 1 The composition prepared by the 4.0 mg / kg administration group, the fifth group was the composition prepared in Example 1 0.80 mg / kg administration group. The first group received free water and the second group received tap water at the same dose.

At this time, the amount of turmeric treatment (0.80-40.0mg / kg) was administered orally once daily to the rat for 2 days each 2ml solution. As a control group, 2 ml of tap water was orally administered, and 0.1 ml of carbon tetrachloride was intraperitoneally injected into the rat in a subsequent administration experiment.

Post-treatment experiments consisted of 0.1 ml of carbon tetrachloride (CCl ₄ , 0.5ml / kg, 50% dissolved in olive solution), a liver toxicity agent, after 24 hours of intraperitoneal administration. Once a day, oral administration was performed for 7 days. After 24 hours, the abdominal midline was dissected under ether anesthesia, and blood was collected from the abdominal aorta.

For the measurement of AST and ALT activity in serum, blood was collected by coagulation of 3 ml of blood collected from the abdominal vein under ether anesthesia at room temperature after 40 minutes at room temperature, centrifuged at 12,000 rpm for 10 minutes at 4 ° C, and serum was separated. Serum AST (GOT) and ALT (GPT) values were measured.

AST (GOT) and ALT (GPT) and other physiological tests include albumin (ALbumin, ALB), globulin (GLOB), alkaline phosphate (ALKP), cholesterol (cholesterol) and total protein in serum. , TP) and total bilirubin (TBIL) measurements were analyzed by Vet test 8008, Dry Chem Parameter (IDEX, USA).

Liver biopsies were collected by ether anesthesia at the end of the experiment, livers were collected, the livers were weighed, and the livers were visually observed. After completion of the experiment, carbon tetrachloride and liver tissue obtained from the treated group were fixed in 10% formalin solution, and then dehydrated and paraffin embedded. Tissue sections 4 to 6 ㎛ thick were made and stained with Hematoxylin & Eosin and observed by light microscopy.

Example 1 is the first group as a result of the serum biochemical test in the post-treatment liver treatment effect experiment using the food composition of the present invention to measure the effect of improving liver function on carbon tetrachloride (CCl ₄ ) The enzyme activities of AST (GOT) and ALT (GPT) were 162.8U / L and 53.2U / L, respectively.

In the control group, which was treated with 2 ml of tap water for 7 days after CCl ₄ treatment, which is a hepatotoxic substance, the enzyme activities of AST (GOT) and ALT (GPT) were 173.8 U / L and 66.8 U / L, significantly increased compared to the normal group. Hepatic damage appeared, which is shown in Table 3.

[ Table 3 ] turmeric and carbon tetrachloride ( CCl ₄ ) Analysis of liver treatment effect of functional health food prepared with a mixture of dongleule extract and maltodextrin

Experimental group The number of animals AST  (GOT, U / L) ALT (GPT, U / L) ALP (U / L) T- BIL  (mg / dl) P (*)  First group (jeong Military ) 5 162.8 ± 15.01bc 53.2 ± 4.71a 128.2 ± 27.36ab <0.1  2nd group (Large Group ) 5 173.8 ± 14.06c 66.8 ± 8.98b 155.4 ± 35.60b <0.1 <0.05  3rd group (40mg / kg) 5 135.6 ± 12.03a 53.0 ± 5.34a 123.2 ± 13.16ab <0.1 <0.05  4th group (4mg / kg) 5 143.6 ± 18.86ab 52.6 ± 11.80a 118.5 ± 9.61a <0.1 <0.05  5th group (0.8mg / kg) 5 148.6 ± 13.83ab 61.8 ± 3.27ab 141.8 ± 19.07ab <0.1 <0.05

SPSS Package, Duncan's multiple range test (P <0.05), Data are the mean ± the standard deviation.

Also, ALB of 3.62g / dl, 3.14g / dl of GLOB, 6.76g / dl of TP, 128.2U / L of ALP (alkaline phosphatase), <0.1mg / dl of TBIL and 87.2mg / of CHOL dl is shown. The control group showed 3.66g / dl, GLOB 2.94g / dl, TP 6.62g / dl, ALKP 155.4U / L, TBIL <0.10mg / dl and CHOL 85.4mg / dl.

However, the enzyme activity of AST (GOT) and ALT (GPT) in the third group, which was administered 40mg / kg concentration after CCl ₄ intraperitoneal injection, was 135.6U / L and 53.0U / L, respectively. In addition, the ALB of the third group administered at the concentration of 40 mg / kg was 3.5g / dl, 3.36g / dl for GLOB, 6.84g / dl for TP, 123.2U / L for ALP, <0.10mg / dl for TBIL and 90.5 mg / dl. After CCl ₄ intraperitoneal injection, the enzyme activities of AST (GOT) and ALT (GPT) in the 4th and 5th groups, which were administered at the concentrations of 4.0 mg / kg and 0.80 mg / kg, were 143.8 U / L and 52.5, respectively. U / L and 148.8 U / L and 61.8 U / L.

In addition, ALB of the fourth group administered at 4.0 mg / kg concentration, 3.08 g / dl, GLOB 3.36 g / dl, TP 6.46 g / dl, ALP 118.6 U / L, TBIL <0.10 mg / dl and CHOL Was 85.25 mg / dl. In the fifth group administered with 0.80mg / kg concentration, ALB of 3.58g / dl, GLOB of 3.0g / dl, TP of 6.56g / dl, ALP of 141.6U / L, TBIL of <0.10mg / dl and CHOL Was 85.0 mg / dl.

As a result of this experiment, the third and fourth groups treated with liquid turmeric extract for 7 days showed lower AST (GOT) and ALT (GPT) than the control group. In addition, ALT (GPT) was not significantly different (P <0.05) compared to the normal group. The ALP (alkaline phosphatase) of the turmeric extract treatment group was not significantly different from the normal group (P> 0.05), and the fourth group showed a significant difference (P <0.05) from the control group.

From these results, it can be seen that the functional food of the present invention lowers the enzyme activity and ALP of AST (GOT), ALT (GPT) elevated by carbon tetrachloride.

Example 2

The turmeric used in this experiment was purchased from Jindosan turmeric powder purchased from the well-being health guard and used for the manufacture of health functional food. The solvent used as turmeric extract was food grade ethanol (GRAS grade, 95% purity), and it was used as sweetness and flavoring substance in domestically dried dongle and maltodextrin (Fine Fiber # 100489, Mitsuda Chemical Industries, Japan). It was purchased from a commercial company and was prepared in a ratio of 1:10:10, turmeric extract, donggulle and maltodextrin mixture into a hard capsule of 500mg weight.

To 100 g of each turmeric powder, 400 g of ethanol (GRAS grade, 95% purity) was put into a 1 L Erlenmeyer flask and stirred using a stirrer (Model 0750, Oriental Science). Stirring speed was 171 r.p.m. It left still for 30 minutes after stirring at room temperature for 3 hours. Thereafter, 1,200 ml of the supernatant was recovered from 300 ml of each 1 L Erlenmeyer flask, and then concentrated under reduced pressure at 65 ° C. for 1 hour using a vacuum distillation unit (Model, Buchi 461 rotary vaccum evaporator, Switzerland). During the experiment, turmeric concentrate was extracted 10 times to prepare up to 200 ml, and 50 ml of this was used as a final hard capsule preparation.

The extract was added to 1,000 g of commercially available domestic donggle dry material, and then heated for 30 minutes to obtain a concentrated solution of 500 ml.

Maltodextrin was used as a liquid ignition ovary maltodextrin (Fine Fiber # 100489, Mitsuda Chemical Co., Ltd., Japan) 500g.

Rats purchased 50 Sprague-Dawley (SD) male rats (weight 260 ± 10g) and went through the 7-day acclimatization period in the experimental animal room to observe the general symptoms during the breeding period. The test was published. Sprague-Dawley (SD) rats were selected as control and treatment groups, and each rat was placed in a random manner. The temperature at the purified breeding room was 22 ℃ (5 ℃ temperature difference), 55% humidity (5% humidity difference), and the illuminance was 150 ~ 200Lux, and the contrast was changed every 12 hours at 7am and 7pm. . Animals were housed in two separate rats in a polycarbonate cage (260WX420LX180H mm, large device) for rats. For feed, rats were given solid feeds (orients) and drinking water was freely available for tap water.

A hard gelatin capsule containing 500 mg of the powder of the mixture of Example 2 as an active ingredient per capsule was prepared by the following method.

Ingredient Content (1000 Capsules)

Turmeric Extract 50ml (Curcumin 8.851 ppm)

500ml Extract

Hwaseong ovary maltodextrin 500g

The liquid turmeric, donggle and maltodextrin mixed composition (hereinafter referred to as the 'composition of the present invention') of the above liquid is dissolved by stirring for 15 minutes with a 180 ± 30 ° C hotplate stirrer, and placed on a hot plate dryer (130 ± 30 ° C) for 0.5 It was heated for about 30 minutes to form a coating having a thickness of ± 0.1 cm. After homogenizing for about 5 minutes with a homogenizer, the composition of the present invention was powdered, and then stored in a freezer at -18 ° C to prepare a hard gelatin capsule.

As a powdery functional material obtained in Example 2, a sample was prepared and used as follows.

Sample 1: A solution in which 5.0 g of a functional substance was suspended in 195 ml of distilled water.

Sample 2: A solution in which 1.0 g of a functional substance was suspended in 199 ml of distilled water.

Sample 3: A solution containing 0.2 g of a functional substance in 199.8 ml of distilled water.

Example 2 Sprague-Dawley male rats to analyze the effect of liver treatment on the enzymatic activity of AST (GOT) and ALT (GPT) in serum of rats that caused liver damage with carbon tetrachloride (CCl ₄ ) (260 ± 10 g body weight) of 9 animals per group.

Group 1: Normal group, Group 2: Control group, Group 3: 192.3 mg / kg administration group, the composition prepared by Example 2, Group 4: 38.5 mg / kg administration group, the composition prepared by Example 2, Group 5: A 7.7 mg / kg administration group of the composition prepared in Example 2 was used.

The first group received free water and the second group received tap water at the same dose. Each 2 ml solution was administered orally to rats once daily for 7 days. As a control group, 2 ml of tap water was orally administered, and 0.1 ml of carbon tetrachloride was intraperitoneally injected into the rat in a subsequent administration experiment.

Post-treatment experiments consisted of 0.1 ml of carbon tetrachloride (CCl ₄ , 0.5ml / kg, 50% dissolved in olive solution), a liver toxicity agent, after 24 hours of intraperitoneal administration. Once a day, oral administration was performed for 7 days. After 24 hours, the abdominal midline was dissected under ether anesthesia, and blood was collected from the abdominal aorta.

For measurement of AST and ALT activity in serum, 3 ml of blood collected from the abdominal vein under ether anesthesia at the end of the experiment was coagulated after 40 minutes at room temperature, centrifuged at 12,000 rpm for 10 minutes at 4 ° C, and serum was separated. AST (GOT) and ALT (GPT) values were measured.

AST (GOT), ALT (GPT) and other physiological test items were measured for total bilirubin (TBIL) in serum using Green Cross Medical Foundation (model ADVIA 1650, Bayer, Japan), Samkwang Medical Foundation, SCL (Medical Foundation Seoul Medical Science). Laboratory) Analyzed by requesting an analysis institution.

Example 2 is a liver toxic substance as a result of a serum biochemical test in the post-treatment liver treatment effect test using each health functional food of Example 2 to measure the liver treatment effect on carbon tetrachloride (CCl ₄ ) Seven days after CCl ₄ intraperitoneal injection, AST (GOT) and ALT (GPT) were analyzed.

The results of analysis using the analyzer of the Green Cross Medical Foundation (model ADVIA 1650, Bayer, Japan) showed that the enzymatic activity of AST (GOT) and ALT (GPT) of normal group was 98.3U / L and 44.9U / L, respectively. Indicated. The control group of the second group which received 2 ml of tap water for 7 days after CCl ₄ treatment, which is a liver toxic substance, increased the enzymatic activity of AST (GOT) and ALT (GPT) to 100.3 U / L and 57.4 U / L compared to the normal group. Hepatic damage was shown, which is shown in Table 4.

[ Table 4 ] turmeric and carbon tetrachloride ( CCl ₄ ) Polygonatum extract and analyze the therapeutic effects of a cross-functional health food manufacturing index to malto bit lean mixture

Experimental group The number of animals AST  (GOT, U / L) ALT (GPT, U / L) P (*) First group (normal group) 9 98.3 ± 9.7U / L 44.9 ± 4.4U / La 2nd group (control group) 9 100.3 ± 18.2U / L 57.4 ± 18.0U / Lb <0.05  3rd group ( Mixtures192 .3 mg / kg) 9 100.3 ± 15.7U / L 52.8 ± 5.3U / Lab <0.05  4th group ( Mixture38 .5 mg / kg) 9 98.9 ± 16.6U / L 48.4 ± 7.5U / Lab <0.05  5th group ( Mixture 7 .7 mg / kg) 9 103.7 ± 23.2U / L 52.9 ± 12.0U / Lab <0.05

Green Cross Medical Foundation Analysis

SPSS Package, Duncan's multiple range test (P <0.05), Data are the mean ± the standard deviation.

However, the enzyme activities of AST (GOT) and ALT (GPT) in the third group, which were administered with 192.3 mg / kg of each functional substance after CCl ₄ intraperitoneal injection, were 100.3 U / L and 52.8 U / L, respectively. The enzyme activities of AST (GOT) and ALT (GPT) in the fourth group, which received 38.5 mg / kg of each functional substance after CCl ₄ intraperitoneal injection, were 98.9 U / L and 48.4 U / L, respectively. Indicated. After CCl ₄ intraperitoneal injection, the enzyme activities of AST (GOT) and ALT (GPT) of the fifth group in which each functional substance was administered at a concentration of 7.7 mg / kg were 103.7 U / L and 52.9 U / L, respectively.

As a result of analysis of liver treatment effect of this experiment, the treatment group of the composition of the present invention of the fourth group showed similar enzymatic activity of AST (GOT) and ALT (GPT) compared to the normal group. The composition treatment group of the present invention did not have a significant difference (P <0.05) for the enzyme activity of ALT (GPT) compared to the normal group, but the control group was significantly higher than the normal group (P <0.05) ALT (GPT) Showed enzymatic activity.

In post-treatment experiment, turmeric mixture treatment group showed no significant difference in ALT (GPT) compared to normal group after 7 days (P> 0.05), but the control group showed no significant difference (P <). 0.05), and the rats to which the composition of the present invention was administered 38.5 mg / kg were more useful for reducing enzymatic activity of ALT (GPT) elevated by carbon tetrachloride.

Example 3

The turmeric used in this experiment was purchased from Jindosan turmeric powder purchased from the well-being health guard and used for the manufacture of health functional food. The solvent used as turmeric extract was food grade ethanol (GRAS grade, 95% purity) and distributed domestically dried dongle and maltodextrin (Fine Fiber # 100489, Mitsuda Chemical Industries, Japan) as a sweetness and flavoring substance. It was purchased from the company and used in a ratio of turmeric, dongle and maltodextrin mixture was prepared as a hard capsule of 500mg weight.

To 100 g of each turmeric powder, 400 g of ethanol (GRAS grade, 95% purity) was put into a 1 L Erlenmeyer flask and stirred using a stirrer (Model 0750, Oriental Science). Stirring speed was 171 r.p.m. It left still for 30 minutes after stirring at room temperature for 3 hours. Thereafter, 1,200 ml of the supernatant was recovered from 300 ml of each 1 L Erlenmeyer flask, and then concentrated under reduced pressure at 65 C for 1 hour using a vacuum distillation unit (Model, Buchi 461 rotary vaccum evaporator, Switzerland) to recover 20 ml of concentrate. During the experiment, turmeric concentrate was extracted 10 times to prepare up to 200 ml, and 50 ml of this was used as a final hard capsule preparation.

The extract was added to 1000L ℃ ℃ to 100g donggulle using a commercially available domestic dry material and then heated for 30 minutes to prepare a concentrated solution of 500ml was used in the experiment.

Maltodextrin 500g of a liquid pyrophoric maltodextrin (Fine Fiber # 100489, Mitsuda Chemical Co., Ltd., Japan) was used.

Rats purchased 50 Sprague-Dawley (SD) male rats (weight 230 ± 10g) and went through a 7-day acclimatization period in the experimental animal room to observe general symptoms during the breeding period. The test was published. Sprague-Dawley (SD) rats were selected as control and treatment groups, and each rat was placed in a random manner. The temperature at the purified breeding room was 22 ℃ (5 ℃ temperature difference), 55% humidity (5% humidity difference), and the illuminance was 150 ~ 200Lux, and the contrast was changed every 12 hours at 7am and 7pm. . Animals were housed in two separate rats in a polycarbonate cage (260WX420LX180H mm, Daejong Instruments) for rats. For feed, rats were given solid feeds (orients) and drinking water was freely available for tap water.

A hard gelatin capsule containing 500 mg of the powder of the mixture of Example 3 as an active ingredient per capsule was prepared by the following method.

Ingredient Content (1000 Capsules)

Turmeric Extract 50ml (Curcumin 8.851ppm)

500ml Extract

Hwaseong ovary maltodextrin 500g

The turmeric, roundley and maltodextrin compositions were dissolved by stirring for 15 minutes with a 180 ± 30 ° C. hot plate stirrer, and then formed into a 0.5 ± 0.1 cm thick film on a hot plate dryer (130 ± 30 ° C.), followed by powdering after heating for 30 minutes. It was. After homogenizing for 5 minutes with a homogenizer, the turmeric mixture was powdered and then stored in a freezer at -18 ° C to prepare a hard gelatin capsule.

As a powdery functional material obtained in Example 3, a sample was prepared and used as follows.

Sample 1: A solution in which 5.0 g of a functional substance was suspended in 195 ml of distilled water.

Sample 2: A solution in which 1.0 g of a functional substance was suspended in 199 ml of distilled water.

Sample 3: A solution containing 0.2 g of a functional substance in 199.8 ml of distilled water.

Example 3 Sprague-Dawley male rats to analyze the effect of liver protection on the enzymatic activity of AST (GOT) and ALT (GPT) in serum of rats that caused liver damage with carbon tetrachloride (CCl ₄ ) (230 ± 10 g body weight) were placed 9 animals per group.

 Group 1: Normal group, Group 2: Control group, Group 3: 217.4 mg / kg administration group, the composition prepared by Example 3, Group 4: 43.5 mg / kg administration group, the composition prepared by Example 3, agent Group 5: 8.7 mg / kg of the composition prepared in Example 3 was administered. The first group received free water and the second group received tap water at the same dose. Each 2 ml solution was administered orally to rats once daily for 7 days. In the control group, 2 ml of tap water was orally administered in a pre-dose experiment, followed by intraperitoneal injection of 0.1 ml of carbon tetrachloride.

In the pre-treatment experiment, each functional substance was orally administered once a day for 7 days, and after 24 hours, 0.1 ml of carbon tetrachloride (CCl ₄ , 0.5ml / kg, 50% olive solution), which is a toxic agent of liver Dissolved in) was administered once intraperitoneally. After 24 hours, the abdominal midline was dissected under ether anesthesia and blood was collected from the abdominal aorta.

For measurement of AST and ALT activity in serum, 3 ml of blood collected from the abdominal vein under ether anesthesia at the end of the experiment was coagulated after 40 minutes at room temperature, centrifuged at 12,000 rpm for 10 minutes at 4 ° C, and serum was separated. AST (GOT) and ALT (GPT) values were measured.

AST (GOT), ALT (GPT), and other physiological tests included total bilirubin (TBIL) measurements in serum. The Green Cross Medical Foundation (model ADVIA 1650, Bayer, Japan), Samkwang Medical Foundation, SCL (Seoul Medical Science Laboratory) The analysis was requested by an analysis institution.

Example 3 is 24 hours after the administration of the composition of the present invention 7 days in the pre-treatment liver protective effect experiment using the health functional food of Example 3 to measure the liver protective effect on carbon tetrachloride (CCl ₄ ) After this, CCl ₄ , a liver toxic substance, was intraperitoneally injected and serum biochemical tests were performed.

The results of analysis using the analyzer of the Green Cross Medical Foundation (model ADVIA 1650, Bayer, Japan) showed that the enzyme activities of AST (GOT) and ALT (GPT) of the normal group of the first group were 90.3U / L and 39.0U / L, respectively. Indicated. Enzyme activity of AST (GOT) and ALT (GPT) in the control group, the second group to which CCl ₄ was administered, was increased to 384.2U / L and 162.9U / L, indicating liver damage. This is shown in Table 5.

[ Table 5 ] turmeric and carbon tetrachloride ( CCl ₄ ) Analysis of Hepatoprotective Effect of Functional Health Foods Prepared from a mixture of Dongle Extract and Maltodextrin

Experimental group The number of animals AST  (GOT, U / L) ALT (GPT, U / L) P (*) First group (normal group) 9 90.3 ± 7.9 39.0 ± 6.0 2nd group (control group) 9 384.2 ± 100.4 162.9 ± 51.7 <0.05  3rd group (mixture 217mg / kg) 9 340.8 ± 107.8 142.4 ± 92.0 <0.05  4th group (mixture 43.5mg.kg) 9 400.8 ± 127.9 196.3 ± 76.3 <0.05  5th group (mixture 8.7mg / kg) 9 439.7 ± 238.4 190.6 ± 114.2 <0.05

Green Cross Medical Foundation Analysis

SPSS Package, Duncan's multiple range test (P <0.05), Data are the mean ± the standard deviation.

However, the enzyme activities of AST (GOT) and ALT (GPT) of the third group, which were pre-treatment of the composition of the present invention at the concentration of 217.4 mg / kg and then intraperitoneally injected with CCl ₄ , were 340.8 U / L and 142.4, respectively. U / L is shown.

The results of this experiment showed that the turmeric mixture treatment group of the third group decreased the enzyme activity of AST (GOT) and ALT (GPT) to 88.7% and 87.5%, respectively, compared to the control group.

From these results, rats pre-treatment of the composition of the present invention, the composition of the present invention, 217.4mg / kg reduced enzyme activity such as AST (GOT) and ALT (GPT) elevated by carbon tetrachloride. Can be.

The results of analysis using the analyzer of Samkwang Medical Foundation (model ADVIA 1650, Bayer, Japan) showed that the enzyme activities of AST (GOT) and ALT (GPT) of normal group were 11.1U / L and 37.2U / L, respectively. Indicated. Enzyme activity of AST (GOT) and ALT (GPT) of the control group, CC2 ₄ control group, respectively, was increased to 388.8U / L and 156.8U / L, indicating liver damage. Table 6 shows.

[ Table 6 ] turmeric and carbon tetrachloride ( CCl ₄ ) Analysis of Hepatoprotective Effect of Functional Health Foods Prepared from a mixture of Dongle Extract and Maltodextrin

Experimental group The number of animals AST  (GOT, U / L) ALT (GPT, U / L) P (*) First group (normal group) 9 91.1 ± 7.0 37.2 ± 5.7 2nd group (control group) 9 388.8 ± 99.3 156.8 ± 49.9 <0.05  3rd group (mixture 217mg / kg) 9 342.1 ± 186.6 137.0 ± 89.5 <0.05  4th group (mixture 43.5mg / kg) 9 400.1 ± 130.7 188.2 ± 73.6 <0.05  5th group (mixture 8.7mg / kg) 9 454.6 ± 270.0 183.8 ± 116.0 <0.05

Samkwang  Medical Foundation Analysis

SPSS Package, Duncan's multiple range test (P <0.05), Data are the mean ± the standard deviation.

However, the enzyme activities of AST (GOT) and ALT (GPT) of the third group, which were pre-treatment of the composition of the present invention at the concentration of 217.4 mg / kg and then intraperitoneally injected with CCl ₄ , were 342.1U / L and 137.0, respectively. U / L is shown.

The results of this experiment showed that the turmeric mixture treatment group of the third group decreased the enzymatic activity of AST (GOT) and ALT (GPT) to 88.0% and 87.4%, respectively, compared to the control group.

The results of analysis using the analyzer of the Seoul Medical Science Foundation (model ADVIA 1650, Bayer, Japan) showed that the enzyme activities of AST (GOT) and ALT (GPT) of the normal group, the first group, were 86.4U / L and 39.0U /, respectively. L is shown. Enzyme activity of AST (GOT) and ALT (GPT) in the control group, the second group to which CCl ₄ was administered, increased to 373.8 U / L and 160.3 U / L, indicating liver damage. This is shown in Table 7.

[ Table 7 ] turmeric and carbon tetrachloride ( CCl ₄ ) Analysis of Hepatoprotective Effect of Functional Health Foods Prepared from a mixture of Dongle Extract and Maltodextrin

Experimental group The number of animals AST  (GOT, U / L) ALT (GPT, U / L) Total protein Albumin Total bilirubin P (*) First group (normal group) 9 86.4 ± 5.9 39.0 ± 5.3 5.7 ± 0.2 3.6 ± 0.1 0.02 ± 0.01a 2nd group (control group) 9 373.8 ± 95.8 160.3 ± 49.7 5.8 ± 0.3 3.7 ± 0.2 0.05 ± 0.01c <0.05  3rd group (mixture 217mg / kg) 9 333.7 ± 180.6 141.9 ± 91.6 5.6 ± 0.3 3.6 ± 0.2 0.03 ± 0.01b <0.05  4th group ( Mixture43 .5 mg / kg) 9 389.3 ± 122.4 192.4 ± 73.3 5.6 ± 0.2 3.6 ± 0.1 0.04 ± 0.01c <0.05  5th group (mixture 8.7mg / kg) 9 427.9 ± 235.7 191.6 ± 126.8 5.6 ± 0.2 3.6 ± 0.1 0.05 ± 0.01c <0.05

SCL Medical Foundation Analysis Results

SPSS Package, Duncan's multiple range test (P <0.05), Data are the mean ± the standard deviation.

However, the administration of the present composition to 217.4mg / kg concentration, and then CCl ₄ intraperitoneal enzymatic activity of AST (GOT) in a third group of injection and ALT (GPT) was characterized by the respective 333.7U / L and 141.9U / L.

The results of this experiment showed that the turmeric mixture treatment group of the third group decreased the enzymatic activity of AST (GOT) and ALT (GPT) to 89.3% and 88.5%, respectively.

In addition, the rat pre-treatment of 217.4 mg / kg of the present invention showed that the total bilirubin content significantly (P <0.05) compared to the control group. From these results, it can be seen that the rat pre-treatment of the present invention composition of the present invention, which is the composition of the present invention, is a useful substance for reducing the enzyme activity and T-bilirubin content enhanced by carbon tetrachloride. These results demonstrate that hard capsules prepared from turmeric, dongle and maltodextrin mixtures can be useful health functional foods that can improve liver function impaired by carbon tetrachloride.

Example 4

The turmeric used in this experiment was purchased from Jindosan turmeric powder purchased from the well-being health guard and used for the manufacture of health functional food. The solvent used as turmeric extract was food grade ethanol (GRAS grade, 95% purity) and distributed domestically dried dongle and maltodextrin (Fine Fiber # 100489, Mitsuda Chemical Industries, Japan) as a sweetness and flavoring substance. It was purchased from the company and used in a ratio of turmeric extract, roundley and maltodextrin mixture to prepare a hard capsule of 500mg weight.

To 100 g of each turmeric powder, 400 g of ethanol (GRAS grade, 95% purity) was put into a 1 L Erlenmeyer flask and stirred using a stirrer (Model 0750, Oriental Science). Stirring speed was 171 r.p.m. It left still for 30 minutes after stirring at room temperature for 3 hours. Thereafter, 1,200 ml of the supernatant was recovered from 300 ml of each 1 L Erlenmeyer flask, and then concentrated under reduced pressure at 65 ° C. for 1 hour using a vacuum distillation unit (Model, Buchi 461 rotary vaccum evaporator, Switzerland). During the experiment, turmeric concentrate was extracted 10 times to prepare up to 200 ml, and 50 ml of this was used as a final hard capsule preparation.

The extract was added to 1,000 g of commercially available domestic donggle dry material, and then heated for 30 minutes to obtain a concentrated solution of 500 ml.

Maltodextrin was used as a liquid ignition ovary maltodextrin (Fine Fiber # 100489, Mitsuda Chemical Co., Ltd., Japan) 500g.

Sensory evaluation for use of the composition of the present invention as a health functional and palatable food was carried out by a 9 point hedonic scale. Odor, appearance and flavor were evaluated to analyze the palatability of the final consumer. The control group scored 5 points, and the treatment group was rated as 9 points for the control group, and 1 to 4 points for the disgusting group, and 1 point for the dislike group.

Rats purchased 50 Sprague-Dawley (SD) male rats (weight 260 ± 10g) and went through the 7-day acclimatization period in the experimental animal room to observe the general symptoms during the breeding period. Was published in the test. Sprague-Dawley (SD) rats were selected as control and treatment groups, and each rat was placed in a random manner. The temperature at the purified breeding room was 22 ℃ (5 ℃ temperature difference), 55% humidity (5% humidity difference), and the illuminance was 150 ~ 200Lux, and the contrast was changed every 12 hours at 7am and 7pm. . Animals were housed in two separate rats in a polycarbonate cage for rats (260WX420LX180H mm, Daejong Instruments). For feed, rats were given solid feeds (orients) and drinking water was freely available for tap water.

A hard gelatin capsule containing 500 mg of the powder of the mixture of Example 4 as an active ingredient per capsule was prepared by the following method.

Ingredient Content (1000 Capsules)

Turmeric Extract 50ml (Curcumin 8.851ppm)

500ml Extract

Hwaseong ovary maltodextrin 500g

The turmeric, roundley and maltodextrin compositions were dissolved by stirring for 15 minutes with a 180 ± 30 ° C. hot plate stirrer, and then formed into a 0.5 ± 0.1 cm thick film on a hot plate dryer (130 ± 30 ° C.), followed by powdering after heating for 30 minutes. It was. After homogenizing for 5 minutes with a homogenizer, the turmeric mixture was powdered and then stored in a freezer at -18 ° C to prepare a hard gelatin capsule.

As a powdery functional material obtained in Example 4, a sample was prepared and used as follows.

Sample 1: A solution of 7.5 g of a functional substance turbidly mixed with 192.5 ml of distilled water.

Sample 2: A solution in which 5.0 g of a functional substance was suspended in 195.0 ml of distilled water.

Sample 3: A solution in which 2.5 g of a functional substance was suspended in 197.5 ml of distilled water.

Example 4 Sprague-Dawley male rats to analyze the effect of liver protection on the enzymatic activity of AST (GOT) and ALT (GPT) in the serum of rats that caused liver damage with carbon tetrachloride (CCl ₄ ) (260 ± 10 g body weight) of 9 animals per group.

Group 1: Normal group, Group 2: Control group, Group 3: 288.5 mg / kg composition composition prepared by Example 4, Group 4: 192.3 mg / kg composition group prepared by Example 4, agent Group 5: 96.2 mg / kg of the composition prepared in Example 4 was administered. The first group received free water and the second group received tap water at the same dose. Each 2 ml solution was administered orally to rats once daily for 7 days. In the control group, 2 ml of tap water was orally administered in a pre-dose experiment, followed by intraperitoneal injection of 0.1 ml of carbon tetrachloride.

In the pre-treatment experiment, each functional substance was orally administered once a day for 7 days, and after 24 hours, 0.1 ml of carbon tetrachloride (CCl ₄ , 0.5ml / kg, 50% olive solution), which is a toxic agent of liver Dissolved in) was administered once intraperitoneally. After 24 hours, abdominal midline was dissected under ether anesthesia and blood was collected from abdominal aorta.

For measurement of AST and ALT activity in serum, 3 ml of blood collected from the abdominal vein under ether anesthesia at the end of the experiment was coagulated after 40 minutes at room temperature, centrifuged at 12,000 rpm for 10 minutes at 4 ° C, and serum was separated. AST (GOT) and ALT (GPT) values were measured.

AST (GOT), ALT (GPT) and other physiological test items were measured for total bilirubin (TBIL) in serum using Green Cross Medical Foundation (model ADVIA 1650, Bayer, Japan), Samkwang Medical Foundation, SCL (Medical Foundation Seoul Medical Science). Laboratory) Analyzed by requesting an analysis institution.

Example 4 is 24 hours after administration of turmeric mixture for 7 days in the pre-treatment liver protective effect experiment using each health functional food of Example 4 to measure the liver protective effect against carbon tetrachloride (CCl ₄ ) In addition, CCl ₄ , a liver toxic substance, was intraperitoneally injected and serum biochemical tests were performed.

The results of analysis using the analyzer of the Green Cross Medical Foundation (model ADVIA 1650, Bayer, Japan) showed that the enzyme activities of AST (GOT) and ALT (GPT) of the normal group were 85.11 U / L and 30.67 U / L, respectively. Indicated. Enzyme activity of AST (GOT) and ALT (GPT) of the control group, the second group to which CCl ₄ was administered, was increased to 295.56U / L and 89.11U / L, indicating liver damage. This is shown in Table 8.

[ Table 8 ] turmeric and carbon tetrachloride ( CCl ₄ ) Analysis of Hepatoprotective Effect of Functional Health Foods Prepared from a mixture of Dongle Extract and Maltodextrin

Experimental group The number of animals AST  (GOT, U / L) ALT (GPT, U / L) Total protein Albumin Total bilirubin P (*) First group (normal group) 9 85.11 ± 14.86 30.67 ± 7.43 5.52 ± 0.63 3.70 ± 0.36 0.20 ± 0.05 2nd group (control group) 9 295.56 ± 113.44 89.11 ± 53.41 5.11 ± 0.24 3.44 ± 0.16 0.24 ± 0.05 <0.05  Group 3 (mixture 288.5 mg / kg) 9 554.89 ± 496.63 207.67 ± 277.29 5.06 ± 0.29 3.44 ± 0.17 0.22 ± 0.05 <0.05  4th group (mixture 192.3mg / kg) 9 247.11 ± 89.04 74.44 ± 25.29 5.31 ± 0.31 3.61 ± 0.21 0.22 ± 0.08 <0.05  Group 5 (mixture 96.2 mg / kg) 9 307.22 ± 208.25 123.78 ± 167.28 5.34 ± 0.24 3.62 ± 0.15 0.27 ± 0.05 <0.05

Green Cross Medical Foundation Analysis

SPSS Package, Duncan's multiple range test (P <0.05), Data are the mean ± the standard deviation.

  However, the enzyme activities of AST (GOT) and ALT (GPT) of the fourth group, which were pre-treated with 192.3 mg / kg of the present composition, were 247.11 U / L and 74.44 U / L, respectively.

The results of this experiment showed that the turmeric mixture treatment group of the fourth group decreased the enzymatic activities of AST (GOT) and ALT (GPT) to 83.6% and 83.5%, respectively, compared to the control group.

Rat pre-treatment of 192.3 mg / kg of turmeric mixture, which is the composition of this experiment, can be seen to decrease the enzyme activity such as AST (GOT) and ALT (GPT) elevated by carbon tetrachloride.

The results of analysis using the analyzer of Samkwang Medical Foundation (model ADVIA 1650, Bayer, Japan) showed that the enzyme activity of AST (GOT) and ALT (GPT) of normal group, which is the first group, was 101.45U / L and 32.67U / L, respectively. Indicated. Enzyme activity of AST (GOT) and ALT (GPT) of control group 2, which was administered CCl ₄ , which was each liver toxicity, was increased to 320.56U / L and 91.44U / L, indicating hepatic damage. This is shown in Table 9.

[ Table 9 ] turmeric and carbon tetrachloride ( CCl ₄ ) Analysis of Hepatoprotective Effect of Functional Health Foods Prepared from a mixture of Dongle Extract and Maltodextrin

Experimental group The number of animals AST  (GOT, U / L) ALT (GPT, U / L) Total protein Albumin Total bilirubin First group (normal group) 9 101.45 ± 32.30a 32.67 ± 7.53 5.13 ± 0.59 2.33 ± 0.21b 0.11 ± 0.03b 2nd group (control group) 9 320.56 ± 118.00ab 91.44 ± 55.25 4.82 ± 0.14 2.13 ± 0.10a 0.10 ± 0.03b  Group 3 (mixture 288.5 mg / kg) 9 600.00 ± 547.35b 210.22 ± 280.70 4.79 ± 0.24 2.12 ± 0.91a 0.07 ± 0.04b  4th group (mixture 192.3mg / kg) 9 262.44 ± 101.65a 76.44 ± 27.60 4.90 ± 0.25 2.20 ± 0.16ab 0.06 ± 0.02a  Group 5 (mixture 96.2 mg / kg) 9 357.11 ± 262.97ab 147.89 ± 186.80 4.94 ± 0.25 2.22 ± 0.13ab 0.10 ± 0.04b

Samkwang  Medical Foundation Analysis

SPSS Package, Duncan's multiple range test (P <0.05), Data are the mean ± the standard deviation.

However, the enzyme activities of AST (GOT) and ALT (GPT) of the fourth group, which were pre-treated with 192.3 mg / kg of the present composition, were 262.44 U / L and 76.44 U / L, respectively.

The results of this experiment showed that the turmeric mixture treatment group of the fourth group decreased the enzyme activities of AST (GOT) and ALT (GPT) to 81.7% and 83.6%, respectively, compared to the control group.

From the above analysis results, the functional substance of the present invention is a rat pre-treatment of the composition of the fourth group of the present invention (192.3 mg / kg), such as AST (GOT) and ALT (GPT) elevated by carbon tetrachloride. It can be seen that the enzyme activity is reduced. In addition, the total bilirubin content of 192.3 mg / kg treated turmeric extract was significantly decreased (P <0.05) compared to the control and other treatment groups.

The results of analysis using the analyzer of the Seoul Medical Science Foundation (model ADVIA 1650, Bayer, Japan) showed that the enzyme activities of AST (GOT) and ALT (GPT) of the normal group, the first group, were 86.0 U / L and 30.56 U /, respectively. L is shown. Enzyme activity of AST (GOT) and ALT (GPT) in the control group, the second group to which CCl ₄ was administered, increased to 297.33 U / L and 89.22 U / L, respectively, indicating liver damage. This is shown in Table 10.

[ Table 10 ] turmeric and carbon tetrachloride ( CCl ₄ ) Analysis of Hepatoprotective Effects of Functional Health Foods Prepared from a mixture of Donguile Extract and Maltodextrin

Experimental group The number of animals AST  (GOT, U / L) ALT (GPT, U / L) Total protein Albumin Total bilirubin First group (normal group) 9 86.00 ± 14.14a 30.56 ± 6.75 5.37 ± 6.52 3.58 ± 0.30 0.03 ± 0.01 2nd group (control group) 9 297.33 ± 113.23ab 89.22 ± 53.11 5.00 ± 0.18 3.38 ± 0.14 0.05 ± 0.01  Group 3 (mixture 288.5 mg / kg) 9 542.11 ± 479.73b 197.44 ± 256.82 4.93 ± 0.22 3.30 ± 0.12 0.06 ± 0.02  4th group (mixture 192.3mg / kg) 9 248.67 ± 88.19a 75.44 ± 25.59 5.16 ± 0.32 3.41 ± 0.21 0.04 ± 0.01  Group 5 (mixture 96.2 mg / kg) 9 336.33 ± 223.71ab 134.11 ± 169.74 5.20 ± 0.28 3.44 ± 0.17 0.07 ± 0.08

SCL Medical Foundation Analysis Results

SPSS Package, Duncan's multiple range test (P <0.05), Data are the mean ± the standard deviation.

 However, the enzymatic activity of AST (GOT) and ALT (GPT) of the fourth group, which was pre-treated with 192.3 mg / kg of the present composition, was 248.67 U / L and 75.44 U / L, respectively.

The results of this experiment showed that the turmeric mixture treatment group of the fourth group decreased the enzyme activities of AST (GOT) and ALT (GPT) to 83.6% and 84.6%, respectively, compared to the control group.

The results of each AST (GOT) and ALT (GPT) were analyzed by the Green Cross Medical Foundation, Samkwang Medical Foundation, and SCL (Seoul Institute of Medical Sciences). The group (192.3 mg / kg) was analyzed (Table 11). As a result of statistical analysis by Duncan's multiple range test (P <0.05) by SPSS Package, the turmeric mixture treatment group showed a significant decrease (P <0.05) of AST (GOT) compared to the control group.

The results of analysis using AST (GOT) and ALT (GPT) analyzers (model ADVIA 1650, Bayer, Japan) showed that the enzyme activities of AST (GOT) and ALT (GPT) of the normal group were 90.89 U / L, respectively. And 30.96U / L. Enzyme activity of AST (GOT) and ALT (GPT) of the control group, CC2 _4, which was administered liver toxicity, was increased to 304.48U / L and 89.93U / L, indicating liver damage. Table 11 shows.

[ Table 11 ] turmeric and carbon tetrachloride ( CCl ₄ ) Analysis of Hepatoprotective Effect of Functional Health Foods Prepared from a mixture of Dongle Extract and Maltodextrin

Experimental group The number of animals AST  (GOT, U / L) ALT (GPT, U / L) First group (normal group) 9 90.89 ± 22.58a 30.96 ± 6.98a 2nd group (control group) 9 304.48 ± 111.01c 89.93 ± 51.79b  3rd group (mixture 192.3mg / kg) 9 252.74 ± 89.78b 75.44 ± 25.34b

Green Cross Medical Foundation, Samkwang Medical Foundation Of SCL Medical Laboratory Analysis Results

SPSS Package, Duncan's multiple range test (P <0.05), Data are the mean ± the standard deviation.

   However, the enzymatic activity of AST (GOT) and ALT (GPT) of the third group, which was pre-treated with 192.3 mg / kg of the present composition, was 252.74 U / L and 75.44 U / L, respectively.

As a result, the third group of the present invention treatment group showed that the enzyme activity of AST (GOT) and ALT (GPT) decreased to 83.0% and 83.9%, respectively, compared to the control group.

In addition, the rat pre-treatment of the composition of the present invention 192.3 mg / kg (Pre-treatment) showed a significant decrease (P <0.05) of the content of AST (GOT) compared to the control group as well as AST (GOT) compared to the control group ) And ALT (GPT) decreased by 17.0% and 18.1%, respectively. From the results of statistical analysis of the data of three institutions obtained from the Green Cross Medical Foundation, the Samkwang Medical Foundation and the SCL (Seoul Medical Laboratory), the composition of the present invention is a composition of the rat raised by carbon tetrachloride. It can be seen that it is a useful health functional substance that can reduce the enzyme activity.

These results demonstrate that hard capsules prepared from turmeric, dongle and maltodextrin mixtures can be useful health functional foods that can improve liver function impaired by carbon tetrachloride.

The composition of the present invention used in the following experiments for the sensory evaluation was prepared by the powdery functional material obtained in Example 4 (turmeric, roundley and maltodextrin mixture; w / v) and the turmeric extract as a control as follows Used.

Group 1 (control): 5.0 g turmeric extract turbidity in 195.0 ml of distilled water (2.50% turmeric extract)

Group 2: A solution in which 7.5 g powder of a functional substance was turbid in 192.5 ml of distilled water (3.75% of the composition prepared in Example 4)

Group 3: A solution in which 5.0 g of a functional substance was turbidly mixed with 195.0 ml of distilled water (composition prepared by 2.50% Example 4)

Group 4: A solution in which 2.5 g of a functional substance was turbidly mixed with 197.5 ml of distilled water (composition prepared according to 1.25% Example 4)

In order to prepare the turmeric extract and the functional material, the sensory evaluation on the appearance, smell and flavor of turmeric, donggle and maltodextrin mixtures used as capsules was carried out.

[Table 12] turmeric extract, Polygonatum, and sensory evaluation of maltodextrin mixtures

      Save   (Work) process Exterior smell zest P (*)  1st group (control group) 5.00 ± 0.00a 5.00 ± 0.00a 5.00 ± 0.00a  Group 2 (3.75% Horn) Compound ) 7.50 ± 0.53b 7.00 ± 0.67b 7.00 ± 0.67b <0.05  Group 3 (2.50% Horn Compound ) 7.50 ± 0.53b 7.80 ± 0.42c 8.00 ± 0.67d <0.05  Group 4 (1.25% Horn Compound ) 7.20 ± 0.63b 7.00 ± 0.67b 7.50 ± 0.53c <0.05

 SPSS Package, Duncan's multiple range test (P <0.05), Data are the mean ± the standard deviation.

Sensory evaluation was performed on a 9 point hedonic scale by 10 trained panelists. The turmeric extract was graded at 5 points, and the present invention composition was rated at 6-9 points, 9 points if it was very good, 1-4 points if it was not good, and 1 point if it was very dislike.

In this experiment, the sensory evaluation of 2.50% (w / v) turmeric, roundley and maltodextrin mixtures showed the highest grade of appearance, odor and flavor.

The control prepared with 2.50% turmeric extract was graded relatively low due to the distinctive strong aroma and irritant odor formation. However, sensory evaluation agents evaluated that the addition of Dongleul extract to turmeric extract was effective in softening turmeric and improving flavor.

In addition, the addition of maltodextrin and the addition of dongleul were considered to contribute to the improvement of consumers' palatability by changing the color of turmeric beverage. According to the sensory evaluation, turmeric, donggle and maltodextrin mixed extracts were graded higher in odor, appearance, and flavor than the control turmeric extract. The mixture of, and donglego and maltodextrin was judged to improve the taste by mildening the flavor and browning the color of the beverage.

Example 5

A single dose of the composition of the present invention and repeated dose toxicity test for 7 days was performed, and LD50 was measured to determine the toxicity upon single dose. The experimental animals were divided into 5 groups, 10 males of 5 males each in Sprague-Dawley rats (weight 270 ± 10g). After that, the mortality rate was calculated 24 hours after administration of the dose set as shown in Table 13 using the clinical dose of the human as the lowest dose group.

[Table 13] 24 hours after first administration of the turmeric extract for a time of rats Mortality rate

10 each Male 5 Female 5 Dose (mg / kg) Number of animals killed 24 hours after drug administration % Mortality Highest dose group M 5 10,000.0 F = 1 10% F 5 Maximum capacity group × 1/3 M 5 3333.3 0 0% F 5 Highest Capacity Group × 1/9 M 5 1111.1 0 0% F 5 Highest Capacity Group × 1/27 M 5 370.3 0 0% F 5 Highest Capacity Group × 1/81 M 5 123.5 0 0% F 5

As can be seen from the experimental results of Table 13, 24 hours after the administration of the present invention, one female rat died in the highest dose group, and no other animals died in the other treatment groups. This highest dose group is 81 times higher than the daily dose of humans, so at least LD50 will be measured at higher doses. In addition, the amount of meal, activity and urine during the test period and observation period are all normal, it is determined that the composition of the present invention can be used as a functional health food without any toxicity and side effects to the human body.

In order to determine the toxicity upon repeated administration of the composition for 7 days, AST (GOT) and ALT (GPT) analysis and weight change were observed after administration period and termination. Experimental animals were Sprague-Dawley rats were set up in four groups including a control group of 10 rats of 5 males each, and the control group was orally administered with 0.01ml / g of distilled water for injection. As a dose, it carried out at the same azeotropy as the dose of a single dose toxicity test.

[ Table 14 ] Rats of Repeated Dose for 7 Days of Turmeric Extract Mortality rate

Experimental group gender The number of animals Dose (mg / kg / day) Number of dead animals until end of administration Mortality rate Control M 5 0 0 0% F 5 0 0% Highest dose group M 5 3333.3 0 0% F 5 0 0% Medium dose group M 5 1111.1 0 0% F 5 0 0% Lowest dose group M 5 370.3 0 0% F 5 0 0%

As shown in Table 14, oral administration of the composition of the present invention for 7 days resulted in no deaths during the test period and no dead animals in the control group.

[Table 15] The body weight change for 7 days of repeated administration of a turmeric extract

Experimental group The number of animals Dose (mg / kg / day) Start date of administration (g) 7th day (g) (P *) Control 10 0 281.2 ± 6.30 333.1 ± 25.87 Highest dose group 10 3333.3 285.4 ± 4.95 338.3 ± 12.60 - Medium dose group 10 1111.1 285.0 ± 5.68 332.6 ± 9.67 - Lowest dose group 10 370.3 284.6 ± 5.74 332.9 ± 6.74 -

SPSS Package, Duncan's multiple range test (P <0.05), Data are the mean ± the standard deviation.

 As shown in Table 15, no significant body weight change was observed with the control group in all of the lowest, medium and highest dose groups.

As shown in Table 16, no statistical increase or decrease of AST (GOT) and ALT (GPT) levels was observed in the serological test compared to the control group, and thus, the long-term administration of the composition of the present invention is not expected to cause toxicity.

[Table 16] Analysis of seven-day repeated administration of a turmeric extract, AST (GOT) and ALT (GPT)

Experimental group The number of animals Dose (mg / kg / day) AST (U / L) ALT (U / L) (P *) Control 10 0 148.9 ± 45.25 74.9 ± 26.44 Highest dose group 10 3333.3 125.4 ± 22.90 66.7 ± 8.44 - Medium dose group 10 1111.1 118.7 ± 14.59 60.6 ± 9.48 - Lowest dose group 10 370.3 132.4 ± 22.68 72.6 ± 10.88 -

SPSS Package, Duncan's multiple range test (P <0.05), Data are the mean ± the standard deviation.

According to the above experimental results, the composition of the present invention did not show any toxicity at doses up to 3333.3 (mg / kg / day), which corresponds to 27 times the daily clinical dose of human, and thus, the composition of the present invention was safe with little toxicity. It could prove to be a functional food.

Example 6

Raw material of turmeric liquid. The data on the characteristics of the components (Structure 1) and the analysis results are shown in Table 17.

[Structure] turmeric minute water curcumin content = 0.354ppm

[ Table 17 ] Analysis of Raw Material Index of Liquid Turmeric Extract

Analysis Item Standard of Sample Analysis Result  Remarks Exterior 10-fold dilution of turmeric extract Yellow liquid calorie Same as above 4.58kcal / 100g carbohydrate Same as above 0.43% Crude protein Same as above 0.70% Crude fat Same as above 0.06% salt Same as above 3.94mg / 100g Solid content Same as above 1.26%

* General No. 10449, Test Report of Korea Food Laboratory (November 8, 2005)

Analysis of raw material composition of powder for turmeric, donggle and maltodextrin hard capsules for preparing hard capsules was made by requesting from Korea Food Laboratory. 1g of turmeric mixture powder was dissolved in 199ml distilled water for analysis of raw materials, and the analysis results are shown in Table 18.

[ Table 18 ] Analysis of Raw Material Index of Turmeric , Dongul and Maltodextrin Mixtures for the Preparation of Hard Capsules

Analysis Item Standard of Sample Analysis Result  Remarks Exterior 10-fold dilution of turmeric extract Yellow liquid calorie Same as above 1.74kcal / 100g carbohydrate Same as above 0.04% Crude protein Same as above 0.43% Crude fat Same as above 0.02% salt Same as above 1.56mg / 100g Solid content Same as above 0.50%

* General 11846, Korea Food Laboratory Test Report (December 6, 2005)

As described above, the turmeric, dongle and maltodextrin mixed compositions of the present invention are significantly lower than the control group in all of the lowest, medium and highest dose groups, as well as significant changes in body weight as compared to the control group in the serological test. GOT) and ALT (GPT) levels were not observed and no toxicity was observed even at 27 times the daily clinical dose of humans. It has been proven to be a safe and functional food and has been effective in improving liver function.

In addition, the present invention is to remove the strong irritant smell peculiar to turmeric, and to satisfy the taste of consumers as a functional food through the improvement of the sensory quality of appearance by the brown change.

As mentioned above, although the specific method of this invention was described in detail using an Example, the scope of a present invention is not limited only to these.

  The present invention can be a very useful food for improving liver function by each component of turmeric, round gourd and maltodextrin, high quality health that can improve the palatability of consumers by improving the taste and aroma of turmeric by round gourd and maltodextrin It is a very useful invention that can provide functional food to all the people.

Claims (3)

In the health functional food composition containing turmeric, round worm, maltodextrin, Health functional food composition using turmeric, donggle and maltodextrin is characterized in that the mixture of turmeric, dongle, maltodextrin in a weight ratio of 1:10:10. According to claim 1, wherein the food composition is a health functional food composition using turmeric, round goose and maltodextrin, characterized in that consisting of a powder contained in a liquid or capsule. According to claim 2, wherein the powder is obtained by dissolving turmeric, roundley and maltodextrin mixed composition with a hotplate stirrer while stirring it, heating it to form a film, and then homogenizing with a homogenizer and pulverizing with a grinder. Health functional food composition using turmeric, tongle and maltodextrin.

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