KR100692253B1 - method for directly extracting lycopen from tomato - Google Patents

Abstract

Disclosed is an extract containing lycopene, the extract of lycopene, which is an antioxidant, by direct grinding, pressing, cold extraction, and hot extraction. The present invention comprises the steps of selecting a tomato strong red color and grinding using a grinding machine to produce a homogeneous tomato grinding liquid; Performing a mixing process of preparing a vegetable oil mixed solution by mixing tomato ground liquid and vegetable oil in a ratio of 2: 1 by weight ratio (%); The vegetable oil mixture is put into a pressurizer rotating at 200 to 500 rpm and compressed, and the temperature of the vegetable oil mixture is leached for 1 to 10 hours at room temperature (25-35 ° C.), and the temperature of the vegetable oil mixture is 60-70 ° C. In the step of leaching for 15 to 24 hours, the leaching process of leaching for 5 to 10 days at a low temperature of the temperature of the vegetable oil mixture is less than 25 ℃; When the leaching process is completed, the oil phase extract formed on the upper part of the vegetable oil mixture is extracted, and the extracted oil phase extract is added to a new tomato grinding solution of the same weight to repeat the mixing and leaching at least two times. It involves the extraction process to be performed.

Description

Method for directly extracting lycopen from tomato}

1 is an HPLC chromatogram for showing the content of lycopene of a conventional lycopene standard.

Figure 2 is an HPLC chromatogram for showing the content of lycopene extracted at room temperature by the direct extraction method of the present invention.

Figure 3 is an HPLC chromatogram for showing the content of lycopene extracted at high temperature by the direct extraction method of the present invention.

Figure 4 is an HPLC chromatogram for showing the content of lycopene extracted according to the type of vegetable oil used in the direct extraction method of the present invention.

The present invention relates to a method for extracting lycopene from a tomato, and more particularly, to a method for directly extracting lycopene, an antioxidant, by crushing, compressing, cold extraction, and hot extraction of tomatoes.

Lycopene is a highly lipophilic purocarotenoid, mainly present in the Lycopersicum, and present in small amounts in other vegetable or algae species. Lycopene is present in tomatoes at a concentration of 30 to 100 ppm, an average of 50 ppm. It is always present as a secondary metabolite in mammals and plays an important role along with the substrates such as vitamin E, vitamin C and other compounds as antioxidants and as free radical phagocytes.

Lycopene is a natural pigment component that represents the characteristic red color of tomatoes, and the antioxidant activity is attracting attention, and double bonds of lycopene are known to exhibit antioxidant effects such as removing free radicals. In particular, ROS (reactive oxygen species), a kind of freeradyl, is known to play a role in causing the development and progression of degenerative diseases. Therefore, many studies have reported that ingesting lycopene may help prevent and treat degenerative diseases.

In addition, research reports that lycopene, which makes red pigment in tomatoes, reduces the risk of developing prostate cancer, and new research in the United States and Europe reports that lycopene plays a role in preventing coronary artery disease. In a study of two Europeans, the highest intake of lycopene was reported to be 50% less likely to have a heart attack than the least ingested group.

It is also known that lycopene also has a protective effect on the skin, and it has been reported that lycopene protects the skin from photodamage of the skin caused by ultraviolet rays. At this time, lycopene inhibits the disappearance of skin cells by epoptosis of skin cells, and it is known that lipofene has a cell damage protection effect by ultraviolet rays. Tomatoes eaten as a diet have also been reported to protect the skin from UV-induced skin erythema.

Using the antioxidant action of lycopene, lycopene-enhanced cosmetics and health foods have been developed and commercialized. Shiseido Co., Ltd. sells various types of lycopene-enriched cosmetics through InnetNet. LycoRed of Israel has launched a powdered natural lycopene processed product. Recently, lycopene is marketed in the form of tablets (eg 10mg / tablet) and promoted for prostate function and cancer prevention.

As a method for extracting lycopene from a conventional tomato, a method using a conventional organic solvent is widely used. Organic solvents used for extraction include ethanol-nucleic acid (4: 3, v / v), acetone-hexane (3: 5, v / v), ethanol-acetone-hexane (2: 1: 3, v / v) , Ethyl acetate-hexane (1: 1, v / v) and ethyl acetate (100%) are used. In the method for extracting lycopene using an organic solvent, an organic solvent is added to tomatoes, and a carotenoid including lycopene is extracted by shaking to dry the organic solvent layer. At this time, the dry residue containing the carotenoid is used in food, cosmetics and medicine.

The evaporation method is used to remove the organic solvent used in the process of extracting lycopene using a conventional organic solvent, the possibility of residues derived from the organic solvent can not be excluded, and lycopene using a high-purity organic solvent Extracting and using the extracted lycopene to manufacture cosmetics, food and medicines may be economically deteriorated.

That is, the problem of the method of extracting lycopene using the conventional organic solvent is that the lycopene extraction process has a large number of steps, there is a possibility of residual organic solvents and impurities exhibiting toxicity, when manufacturing cosmetics using the extracted lycopene It is necessary to dissolve the lycopene dry residue again, when dissolving lycopene from the dry residue can not guarantee the solubility of 100%, the problem that can not present a variety of extraction methods remains a problem.

Another method of extracting lycopene from tomato was developed by LycoRed of Israel, but it is possible to extract carotenoids containing natural lycopene from tomatoes using oil dispersion. There is a problem that the oil dispersion can not be directly used as food or medicine.

The conventional method of extracting lycopene from tomatoes has a problem of lowering economic efficiency by going through various processes including a powder manufacturing process, and there is a problem of remaining of toxic organic solvent and impurities, thereby simplifying the extraction process of lycopene. There is a need for improvements in methods that can maintain the stability of lycopene.

The present invention was invented to solve the above problems, the present invention is appropriate amount of one vegetable oil of squalane, safflower seed oil, camellia oil, macadamia nut oil, jojoba oil, sunflower oil, sesame oil, avocado oil By adding lycopene directly by crushing, cold sedimentation, or hot sedimentation, the grinding fluid added with vegetable oil has an excellent extraction efficiency when lycopene is extracted, and it improves the economic efficiency and toxic to lycopene extracted using vegetable oil. The object is to provide a method for extracting lycopene directly from tomatoes, which excludes the possibility of residual organic solvents and impurities.

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The present invention for achieving the above object

Selecting a tomato having a strong red color and pulverizing using a crusher to produce a homogeneous tomato crushing solution;

Performing a mixing process of preparing a vegetable oil mixed solution by mixing tomato ground liquid and vegetable oil in a ratio of 2: 1 by weight ratio (%);

The vegetable oil mixture is put into a pressurizer rotating at 200 to 500 rpm and compressed, and the temperature of the vegetable oil mixture is leached for 1 to 10 hours at room temperature (25-35 ° C.), and the temperature of the vegetable oil mixture is 60-70 ° C. In the step of leaching for 15 to 24 hours, the leaching process of leaching for 5 to 10 days at a low temperature of the temperature of the vegetable oil mixture is less than 25 ℃; And

After the leaching process is completed, extract the oil phase extract formed on the upper part of the vegetable oil mixture, and add the extracted oil phase extract to a new tomato grinding solution of the same weight to repeat the mixing process and the leaching process at least two times. To include the extraction process.

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings.

Attached Figure 1 is an HPLC chromatogram for showing the content of lycopene of the conventional lycopene standard, Figure 2 is an HPLC chromatogram for showing the content of lycopene extracted at room temperature by the direct extraction method of the present invention, Figure 3 It is an HPLC chromatogram for showing the content of lycopene extracted at high temperature by the direct extraction method of the present invention.

In addition, Figure 4 is an HPLC chromatogram for showing the content of lycopene extracted according to the type of vegetable oil used in the direct extraction method of the present invention.

The present invention reduces the extraction process, improve the stability and safety of lycopene by directly extracting the extraction method of lycopene directly from the tomato using the vegetable oil allowed in food, and provides an environmentally friendly lycopene extraction method

By using this efficient method of extracting lycopene, natural ingredients of carotenoids, including lycopene, which is an antioxidant component of tomato, are useful for cosmetics, food and pharmaceutical preparations, and anti-aging of skin and atopic dermatitis by using lycopene's health function. It can be applied to cosmetics, medicines and food preparations that impart health functionalities including treatment, sunscreen and damage treatment, psoriasis treatment, skin rash and itching, anti-inflammatory and antibacterial effect.

How to extract lycopene from tomatoes

Tomatoes with strong red color are selected from normal tomatoes, cherry tomatoes, and ripened tomatoes, and homogenized tomato grinding liquid is prepared by homogenization using a grinding machine. Tomato grinding solution thus prepared is stored at a low temperature (0 ~ 4 ℃) until the extraction of lycopene, it is not stored for more than 7 days.

A vegetable oil is mixed with tomato groundwater in a ratio of 2: 1 by weight ratio (%) to perform a mixing process for preparing a vegetable oil mixed solution. At this time, the vegetable oils used are food oils such as squalane, safflower seed oil, camellia oil, macadamia nut oil, jojoba oil, sunflower oil, sesame oil and avocado oil. That is, the tomato grinding liquid and one vegetable oil of the vegetable oil are mixed in a ratio of 2: 1 by weight ratio (%), for example, 50 g of vegetable oil and 100 g of tomato grinding liquid are mixed to prepare a mixed liquid.

The mixed solution is put into a compactor rotating at 200 to 500 rpm and compressed, and then a leaching process is performed. In the leaching process, the mixed liquid is leached for 1 to 10 hours at room temperature (25-35 ° C.), and the mixed liquid is leached for 15 to 24 hours at a high temperature within the range of 60-70 ° C. and the mixed liquid is 25 ° C. or lower. In leaches for 5 to 10 days.

At the end of the leaching process, an oil phase extract separated from tomato groundwater is formed by the oil component contained in the vegetable oil, and the extraction process extracts the oil phase extract first. The extracted oily liquid is added to a new tomato grinding solution of the same weight, and the mixing and leaching are repeated, and the extraction is carried out at least twice.

In other words, when the tomato ground liquor and vegetable oil are mixed, compressed and leached, the oil phase extract is separated. When the primary extraction process for extracting the oil phase extract is completed, the oil phase obtained by the primary extraction process is obtained. A second extraction process of extracting the separated oily extract by performing the same mixing process and leaching process as described above by mixing the extract and the new tomato grinding liquid of the same weight as the tomato grinding liquid used initially. In this way, the extraction process is repeated by repeating the extraction at least twice. The extraction process is carried out at a constant temperature condition in a shaded state, the number of extraction is carried out depending on the type and state of the tomato, but at least two times.

The extraction end point is determined by the intrinsic red intensity of lycopene, where the strength of the color of the extract is proportional to the content of lycopene in the extract.

Lycopene Analysis Method and Content

The lycopene fraction is separated and analyzed using a separating funnel from the oily extract extracted through the repeated extraction process. Lycopene assays include High Performance Liquid Chromatography; Hereinafter referred to as HPLC). The HPLC system (pump, detector, automatic dispenser, system controller, chromatogram program) was used by Shimadzu Corporation (Japan), and the detector used a UV detector to detect lycopene. Lycopene standard (Sigma, 1mg) was used for content determination, and methanol-acetonitrile-THF (70: 25: 5, v / v) was used for the mobile phase solvent.

Lycopene-containing tomato extract was diluted 50-fold (mobile phase solvent) in a 50-mL sample when analyzed using HPLC as an oil phase. Sample injection was performed using an autoinjector system, and the HPLC column for sample separation was a C18-reverse column (Waters, Radial-Pack column). The HPLC was turned off and analyzed under shading conditions. The temperature of the sample was maintained at 4 ° C.

As shown in Figure 1, Figure 2 and Figure 3, the extract extracted at room temperature and high temperature in the direct extraction method of the present invention when the extraction process is repeated the lycopene 5-20%, beta-carotene based on the weight% It was found that this 5-20%, natural tocopherol is present in the range of 5-20%. Here, the content of lycopene extracted by the extraction method of the present invention can be seen that the content of lycopene increased compared to the conventional lycopene standard of Figure 1, as shown in Figure 4, that is, by type of vegetable oil, that is, camellia oil, squalane The content of lycopene extracted using safflower seed oil was almost the same.

Establish stability

In order to establish the stability of the lycopene extracted by the direct extraction method according to the present invention, the storage container uses a brown container with little interference by light, and keeps the lycopene oil extract liquid at 40 ° C. for 4 weeks to periodically change the content of lycopene. As a result, it was possible to maintain the stability of the extracted lycopene for a long time using the direct extraction method using the vegetable oil according to the present invention.

Cosmetic manufacturing

As described above, a method of manufacturing cosmetics as an example of applying the extract of lycopene extracted by directly adding vegetable oil to a product is as follows. The manufacture of cosmetics is divided into a water phase and an oil phase, and a raw material is weighed, and it mixes by stirring. The aqueous phase contains 0.02 g of EDTA-2Na, 0.1-5 g of Witch-Hazel, 1-10 g of glycerine, 0.05-4 g of methylparaban (MP), 0.1-4 g of Carbopol 934, and 0.01-2 g of Xanthan gum per 100 ml of deionized distilled water. do.

Oil phase is Squalane 2-15g, palmityl alcohol 0.1-5g, stearic alcohol 0.1-5g, stearic acid 0.1-5g, Bee wax 0.01-2g, Alracel 165 0.5-5g, Tego Care 450 1-5g, dimethicone 0.1- 4 g and lycopene extract contain 0.5-10 g. The aqueous phase and the emulsion are dissolved at 80 ° C., respectively, and the aqueous phase is mixed at 80 ° C. for 5-15 minutes at 2500 rpm. The emulsion is added to the emulsion and the emulsion phase is reacted with a homomixer at 50-90 ° C for 5-30 minutes. If the homomixer (10 minutes-1 hour) and rotation speed (100-1000 rpm) are adjusted to form micelles and liposomes, and under conditions that produce lamellar layers under a microscope, 50-100 nanometers are used. A metric micron unit is created.

After emulsification, sodium hydroxide solution is added to saponification at 70 ° C. After the saponification reaction, the mixture is cooled at 45 ° C., and 0.01-0.5 g of falvor is added after the cooling is completed. After scavenging, sterilization and aging are performed at room temperature for 1-8 hours.

As described above, the lycopene direct extraction method according to the present invention simplifies the manufacturing process by extracting directly using vegetable oil that does not include the disadvantages of the conventional lycopene extraction method, oily lycopene extract is a problem in the existing method It is possible to maintain the stability of lycopene by inhibiting the degradation of lycopene present, and by using an acceptable vegetable oil that can be used in food, oily lycopene extract can be directly applied to cosmetics, food and pharmaceutical preparations. In addition, it has the effect of laying the foundation for creating high value added for tomato farmers.

Although the preferred embodiments of the present invention have been described in detail with reference to the accompanying drawings, the present invention is not limited thereto and may be improved or modified by those skilled in the art within the scope of the technical idea of the present invention.

Claims (5)

Iii) screening tomatoes with a strong red color and grinding them using a grinding machine to produce a homogeneous tomato grinding solution; Ii) mixing the tomato ground liquor with vegetable oil in a weight ratio (%) in a ratio of 2: 1 to prepare a vegetable oil mixed solution; Iii) The vegetable oil mixture is put into a pressing machine rotating at 200 to 500 rpm and compressed, and the temperature of the vegetable oil mixture is leached for 1 to 10 hours at room temperature (25-35 ° C.), and the temperature of the vegetable oil mixture is 60-. Performing a leaching process for leaching at a high temperature within a range of 70 ° C. for 15 to 24 hours, and leaching for 5 to 10 days at a low temperature where the temperature of the vegetable oil mixture is 25 ° C. or less; And Iii) When the leaching process is completed, extract the oil phase extract formed on the upper part of the vegetable oil mixture, and add the extracted oil phase extract to a new tomato grinding solution of the same weight to at least mix and leach. Method for extracting lycopene directly from the tomato, including the extraction process performed repeatedly two or more times. The method of claim 1, wherein the vegetable oil used in step ii is any one of food oils squalane, safflower seed oil, camellia oil, macadamia nut oil, jojoba oil, sunflower oil, sesame oil, avocado oil is lycopene How to extract yourself. delete delete delete

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