KR100611685B1 - Agitation device used in microorganism cultivation capable of oxygen supply with suppression of foaming - Google Patents

Abstract

An apparatus for agitating a microbial culture solution contained in an agitating tank, the apparatus comprising: an oxygen inlet tube, one end of which is coupled to an external oxygen tank and the other end of which is submerged in the microbial culture solution, for supplying oxygen to the microbial culture solution; A cylinder block coupled to the other end of the oxygen injection pipe, having a first hole penetrating from an inner circumference to an outer circumference and for discharging oxygen to the outside; The first hole is formed in the periphery of the cylinder block, the propeller is provided on the outer periphery, and includes an impeller formed with a second hole that is obliquely penetrated radially from the inner periphery to the outer periphery, the first hole Oxygen discharged from is passed through the second hole is provided to the outside of the impeller is provided with a microorganism culture apparatus capable of supplying a stirrer.

Microbial culture, stirring, oxygen supply

Description

Agitation device used in microorganism cultivation capable of oxygen supply with suppression of foaming}

1 is a schematic diagram showing a state of oxygen supply required for conventional microbial culture;

Figure 2 is an exploded perspective view of the stirring device of the microorganism culture capable of supplying oxygen according to the first embodiment of the present invention;

Figure 3a is a cross-sectional view of the stirring device of the microorganism culture capable of supplying oxygen according to the first embodiment of the present invention;

3B is a cross sectional view taken along the direction A-A of FIG. 3A;

Figure 4 is a partial cross-sectional view showing the operating state of the stirring apparatus of the microorganism culture capable of supplying oxygen according to the first embodiment of the present invention;

5 is an exploded perspective view of a stirring apparatus for culturing microorganisms capable of supplying oxygen according to a second preferred embodiment of the present invention;

Figure 6 is an exploded perspective view of a stirring device for culturing microorganisms for foaming inhibition according to a third embodiment of the present invention;

7 is a cross-sectional view of a stirring apparatus for culturing microorganisms for foaming inhibition according to a third embodiment of the present invention; And

Figure 8 is a cross-sectional view showing the main part showing the process of inhibiting the foaming in the stirring apparatus of the microbial culture for foaming suppression according to a third embodiment of the present invention.

* Explanation of symbols for main parts of the drawings

100: stirring tank 102: oxygen

110: microbial culture 120: oxygen injection tube

130: impeller coupling block 132: impeller insertion cylinder

134: oxygen flow path 134a: oxygen outlet

136: snap ring coupling groove 138: hollow part

140: impeller 142: rotating hole

144: propeller 146: rotating groove

150: snap ring 200: bubble breaking block

202: stopper coupling groove 210: culture medium induction part

212: bubble breaking hole 214: culture fluid inlet

300: air discharge block 310: oxygen inlet

320: air outlet 324: bubble removing agent

326: tapered portion

The present invention relates to a stirrer for microbial culture capable of supplying oxygen, and more particularly, to culture cultured tissues with no power using oxygen or air supplied while suppressing bubble generation due to injection of oxygen or air during aerobic microbial culture. It relates to a stirrer of microbial culture capable of supplying oxygen that can be stirred.

Aerobic microorganisms require oxygen for growth, and when cultured microorganisms need oxygen or air to the medium. This process of oxygen supply will be described with reference to the drawings. 1 is a schematic diagram showing a state of oxygen supply required for conventional microbial culture.

As shown in FIG. 1, oxygen supply for the conventional microbial culture is performed through the oxygen inlet 522 after the oxygen injection tube 520 is inserted into the agitation tank 500 containing the cells related to the culture medium and the tissue culture. Accordingly, oxygen bubbles 512 are generated in the medium in the culture tank 500.

Since the conventional oxygen supply process has only a process of supplying oxygen to the medium, the stirring process required for rapid and accurate microbial culture should be performed on a separate stirring device. The stirring device is mainly a device in which the shaking and locking functions are respectively or simultaneously performed.

Therefore, not only the space occupied by the stirrer is necessary when culturing the microorganism, but there is a problem in that a separate stirrer is purchased.

Meanwhile, bubbles 514 are generated from a highly viscous medium in the process of allowing oxygen or air 512 to be evenly injected into the medium 510 necessary for aerobic microbial culture. Bubbles generated from the medium prevents the effective dissolution of air into the medium to inhibit the growth of the microorganisms, there is a problem that leads to inadequate culture of the microorganisms, such as increased contamination potential.

In order to solve this problem, Patent Application No. 1019850005603 introduces a non-bubble gas treatment device into a liquid culture medium, and the microbial culture is carried out through gas introduction and gas removal without mechanical damage to the components of the liquid medium such as cells. Proliferation is shown. That is, in the non-bubble gas treatment apparatus of a fluid culture medium having a hollow cylindrical thin film cage elastically suspended in a medium to be gas-treated and moved in a translational motion, and gas exchange is performed in the immersed thin film sheet to promote microbial culture. It is proposed that a thin film cage is provided so that there is no moisture before it is formed by one or more hollow thin film filaments and used for the reaction medium.

However, such a configuration has a problem that the structure is not only complicated, but also the durability is poor by mechanical movement.

In addition, due to the complexity of the structure there is a problem of high manufacturing cost.

Accordingly, an object of the present invention is to provide a stirring device for culturing microorganisms capable of supplying oxygen, which does not require a separate stirring device occupying space by simultaneously supplying and stirring oxygen.

In addition, another object of the present invention is to provide an agitation apparatus for microbial cultivation capable of supplying oxygen, which allows rapid microbial cultivation by simultaneously performing an oxygen supply process and a stirring process required for microbial culture.

In addition, another object of the present invention is to provide a stirring device for culturing microorganisms that can suppress the generation of bubbles to the maximum while being durable.

In addition, another object of the present invention is to provide a stirring device for culturing microorganisms that can suppress the generation of bubbles in a simple structure in a state where no separate power is provided.

In addition, another object of the present invention is to provide a stirring device for culturing microorganisms capable of supplying oxygen that can reduce the manufacturing cost of the stirring device.

According to the first aspect of the present invention, in the apparatus for agitating a microbial culture solution contained in a stirring tank, one end is coupled to an external oxygen tank and the other end is immersed in the microbial culture solution, and an oxygen injection tube for supplying oxygen to the microbial culture solution is provided. and; A cylinder block coupled to the other end of the oxygen injection pipe, having a first hole penetrating from an inner circumference to an outer circumference and for discharging oxygen to the outside; The first hole is formed in the periphery of the cylinder block, the propeller is provided on the outer periphery, and includes an impeller formed with a second hole that is obliquely penetrated radially from the inner periphery to the outer periphery, the first hole Oxygen discharged from is passed through the second hole is provided to the outside of the impeller is provided with a microorganism culture apparatus capable of supplying a stirrer.

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In addition, the first hole and the second hole is preferably made of a plurality.

In addition, the impeller is preferably supported by a snap ring coupled outside the impeller on the outer circumference of the cylinder block so that the impeller does not deviate from the cylinder block.

On the other hand, according to a second aspect of the present invention, in the agitation apparatus of the microbial culture solution contained in the stirring tank, one end is coupled to the external oxygen tank, the other end is immersed in the microbial culture medium, supplying oxygen to the microbial culture solution An oxygen injection pipe for; A cylinder block coupled to the other end of the oxygen injection pipe, penetrating from the inner circumference to the outer circumference, and having a hole for discharging oxygen to the outside; Located in the periphery of the cylinder block in which the hole is formed, and provided with a propeller on the outer periphery, an impeller extending diagonally from the top to the lower periphery on the inner periphery and having grooves made of floors and valleys, the oxygen discharged from the hole There is provided a stirring apparatus for culturing microorganisms capable of supplying oxygen, which is discharged to the outside of the impeller along the grooves.

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According to the third aspect of the present invention, in a microorganism culture stirring device accommodated in a stirring tank, it is coupled to the stopper of the stirring tank, and the foam generated from the stirring tank passes and applies a compressive force greater than the surface tension of the foam. A bubble breaking block in which a bubble breaking hole is formed; And an air discharge block coupled to an inner circumference of the bubble breaking block, extending a tube for supplying oxygen through the bubble breaking block, and having an outlet for discharging gas generated as the bubble breaks. The bubble breaking hole is provided with an oxygen supplying microorganism stirrer which has a conical taper shape that narrows from the outer circumference to the inner circumference of the foam breaking block so that the pressure is increased with respect to the foam passing therethrough.

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In addition, the space between the inner periphery of the bubble breaking block and the outer periphery of the air discharge block is preferably formed so that a compressive force is applied to the foam passing through the hole.

In addition, the lower portion of the air discharge block is formed with a hollow portion, the outlet is preferably in communication with the hollow portion.

In addition, the upper portion of the outlet is preferably a tapered first groove extending to the outer periphery of the air discharge block.

In addition, the end of the first groove is preferably formed with a second groove that can accommodate the defoamer.

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings.

2 is an exploded perspective view of an agitator for microbial culture capable of supplying oxygen according to a first preferred embodiment of the present invention, and FIG. 3A is an agitator for microbial culture capable of supplying oxygen according to a first preferred embodiment of the present invention. It is sectional drawing, FIG. 3B is sectional drawing along the AA direction of FIG. 3A.

As shown in Figure 2 to 3b, the agitating apparatus of the microorganism culture capable of supplying oxygen according to the first embodiment of the present invention is a culture medium (110; see FIG. 4) consisting of a tissue such as a medium and cells required for the culture The oxygen injection tube 120, the oxygen injection tube 120 is immersed in the accommodated stirring tank 100 is coupled to the impeller coupling block 130 for stirring the culture medium 110 and supplying the oxygen required for the tissue, and the snap ring (150) It is made of an impeller 140 is inserted into the outer periphery of the impeller coupling block 130 and rotated.

Oxygen infusion pipe 120 is one end is coupled to the external oxygen tank like a conventional oxygen injection pipe is immersed in the culture solution 110 in the stirring tank (100).

Impeller coupling block 130 has a shape of a two-stage structure of the outer periphery cylindrical. An upper portion of the impeller coupling block 130 has a hollow portion 138, and the other end of the oxygen injection pipe 120 is tightly coupled into the hollow portion 138. The lower part of the impeller coupling block 130 is provided with an impeller insertion cylinder 132 having a cylindrical shape of the inner diameter having a tolerance that the inner periphery of the impeller 140 can be inserted and rotated. The inside of the impeller insertion cylinder 132 has an oxygen induction path 134 so that oxygen supplied from the oxygen injection pipe 120 to the hollow portion 138 of the impeller coupling block 130 is supplied to the inside of the impeller insertion cylinder 132. Equipped. At this time, the impeller insertion cylinder 132 has an oxygen discharge port 134a penetrated inside and outside so that oxygen supplied into the impeller insertion cylinder 132 is discharged to the inner circumference of the impeller 140.

After the impeller 140 is inserted into the impeller insertion cylinder 132, the snap ring 150 is coupled to the snap ring coupling groove 136 to prevent the impeller 140 from escaping.

On the other hand, the impeller 140 is provided with a propeller 144 for stirring the culture solution 110 on the outer circumference. Between the inner periphery and the outer periphery of the impeller 140, as shown in Figure 3b is formed a rotation hole 142, which is an obliquely curved oxygen passage.

That is, the direction of the rotation hole 142 is perpendicular to the outer circumference from the inner circumference of the impeller 140 so that the oxygen discharged from the oxygen outlet 134a of the impeller coupling block 130 can rotate the impeller 140. It is not made to be made but at an angle of bending.

The stirring action of the culture solution by the rotating hole 142 of the impeller 140 will be described.

Figure 4 is a partial cross-sectional view showing the operating state of the stirring apparatus of the microorganism culture capable of supplying oxygen according to the first embodiment of the present invention.

As shown in Figure 3b and 4, the impeller coupling block 130 and the impeller 140 in the culture medium 110 in the stirring tank 100, the impeller from the oxygen injection pipe 120 (see Fig. 3a) When oxygen necessary for microbial culture is injected into the oxygen induction path 134 of the coupling block 130, oxygen passing through the oxygen outlet 134a applies a rotational force to one side wall of the rotation hole 142 of the impeller 140. Done. The rotation force generated as described above rotates the propeller 144 by rotating the impeller 140 and at the same time oxygen 102 is supplied to the culture medium 110 through the rotation hole 142.

As described above, the culture solution may be stirred while supplying oxygen necessary for culturing the microorganisms to the culture tissue.

Although one surface of the rotating hole structure of the impeller 140 has been described above, an impeller structure that is more easily manufactured will be described below.

5 is an exploded perspective view of a stirring apparatus for culturing microorganisms capable of supplying oxygen according to a second exemplary embodiment of the present invention.

As shown in FIG. 5, the stirring device for culturing microorganisms capable of supplying oxygen according to the second exemplary embodiment of the present invention has an internal structure of the impeller 140 according to the first exemplary embodiment of the present invention illustrated in FIG. 2. Except for the same structure.

Therefore, the stirring apparatus of the microbial culture capable of supplying oxygen according to the second embodiment having the same configuration as that of the first preferred embodiment of the present invention will be denoted by the same reference numerals as the first embodiment, and with respect to parts having different configurations. Let's explain.

The interior of the impeller 140 of the stirring apparatus for culturing microorganisms capable of supplying oxygen according to the second preferred embodiment of the present invention is different from the top of the floor 146a from the top of the rotating hole 142 (see FIG. 3B). ) And a groove 146 composed of a valley 146b. At this time, the ridge 146a and the valley 146b extend from the top to the bottom in the impeller 140 in a substantially diagonal shape.

As such, the oxygen discharged from the oxygen outlet 134a of the impeller coupling block 130 by the floor 146a and the valley 146b of the impeller 140 formed in an oblique line forms the floor 146a and the valley 146b. The rotational force can be applied with respect to the

On the other hand, it will be described a third preferred embodiment of the present invention having a structure that can suppress the generation of bubbles due to the injection of oxygen or air during aerobic microorganism culture.

At this time, by having the following additional components for the upper structure of the upper portion of the oxygen injection pipe 120 and the stirring vessel 100 will be described in detail with respect to the part that can suppress the bubble generation in addition to the above effects.

Figure 6 is an exploded perspective view of a stirring apparatus for culturing microorganisms for foaming suppression according to a third preferred embodiment of the present invention, Figure 7 is agitation of microbial culture for foaming suppression according to a third embodiment of the present invention Figure 8 is a cross-sectional view of the device, Figure 8 is a cross-sectional view showing the main part showing the process of inhibiting the foaming in the stirring apparatus of the microbial culture for foaming suppression according to a third embodiment of the present invention.

As shown in Figure 6 to 8, the stirring device for culturing microorganisms for foam generation suppression according to a third embodiment of the present invention is a bottle-shaped stirring tank 100, the stopper of the stirring tank 100 It is coupled to the sphere 102 and the bubble breaking block 200 for primarily destroying the bubble, coupled to the inner circumference of the bubble breaking block 200 is supplied to the culture medium 110 of the stirring tank 100 and 2 It is made of an air discharge block 300 for automatically discharge the gas by removing the bubbles.

The foam breaking block 200 has a stopper coupling groove 202 to be tightly coupled to the stopper 102 of the stirring tank 100 to correspond to the stopper 102. A culture solution guide portion 210 is formed in the downward direction along the inner circumference of the circumference of the stopper coupling groove 202 is formed. The bubble breaking hole 212 penetrates through the outer circumferential surface and the inner circumferential surface of the culture solution induction part 210. The bubble breaking hole 212 has a conical taper shape that narrows as it penetrates from the outer circumference of the culture solution induction portion 210 to the inner circumference. Continuous bubbles generated by the supply of oxygen into the stirring tank 100 and the generation of the gas according to the assimilation of the culture tissue are led to the bubble breaking hole 212. At this time, bubbles passing through the bubble breaking hole 212 by the tapered shape of the bubble breaking hole 212 are continuously generated in the stirring vessel 100 and are destroyed by the driving force of the rising bubbles. That is, the bubbles 104 continuously generated in the stirring vessel 100 apply pressure to the bubbles in the bubble breaking hole 212. At this time, it is preferable that the taper shape of the bubble breaking hole 212 is determined so as to apply a pressure greater than the surface tension of the bubble in the bubble breaking hole 212. This phenomenon occurs more effectively as the bubbles generated in the stirring tank 100 increases. The relatively small bubbles generated in the stirring tank 100 may pass through the bubble breaking hole 212 but between the inner circumference of the bubble breaking block 200 located near the lower outer circumference of the air discharge block 300. The space between them is preferably maintained so as to be destroyed by a propulsion force larger than the surface tension of the culture tissue bubbles in the space of the secondary. The foam solution of the culture tissue tissue destroyed in this process flows through the culture solution inlet 214 of the culture solution induction part 210 and flows down to the stirring tank 100.

The air discharge block 300 is formed with an oxygen inflow passage 310 in which the upper and lower portions communicate with each other to supply oxygen into the stirring vessel 100. On the other hand, the outer periphery for close contact with the upper inner circumference of the bubble breaking block 200 is formed in the air discharge block (300). The flange portion 320 extends on the inner circumferential upper portion of the air discharge block 300 in close contact with the inner circumference of the foam breaking block 200. The lower portion of the air discharge block 300 has a hollow portion 328 is formed between the oxygen inlet passage 310 and the outer periphery thereof. The hollow part 328 is provided with an air outlet 322 in communication with the upper portion of the flange part 320. The upper portion of the air outlet 322 is formed with a tapered taper portion 326 approximately perpendicular to the air outlet 322. At the end of the taper portion 326, a bubble removing agent accommodating portion 324 may be formed to accommodate the bubble removing agent.

As described above, the culture tissue passed through the bubble breaking hole 212 and the fine residual bubbles are discharged together with the assimilation gas and excess oxygen of the microbial culture from the hollow portion 328 through the air outlet 322. At this time, the remaining foam is discharged in contact with the bubble removing agent contained in the bubble removing unit 324 is destroyed.

As described above, bubbles caused by microbial culture may be naturally removed.

Therefore, according to the present invention, by supplying and stirring oxygen at the same time, it is possible to enable oxygen supply and agitation to a culture solution required for culturing microorganisms without requiring a separate agitator space. In addition, according to the present invention, the oxygen supply process and the stirring process necessary for culturing the microorganisms can be performed at the same time, thereby enabling rapid microbial culturing and reducing the manufacturing cost of the stirring apparatus.

In addition, according to the present invention is provided with a container for culturing microorganisms that are durable and can suppress the generation of bubbles as much as possible. It is also possible to lower the manufacturing cost of the container by removing the foam in a manner in which no separate power is provided.

Claims (12)

In the stirring device of the microbial culture solution contained in the stirring tank, An oxygen injection tube, one end of which is coupled to an external oxygen tank and the other end of which is immersed in the microbial culture, for supplying oxygen to the microbial culture; A cylinder block coupled to the other end of the oxygen injection pipe, having a first hole penetrating from an inner circumference to an outer circumference and for discharging oxygen to the outside; It is located around the cylinder block formed with the first hole, the propeller is provided on the outer periphery, and includes an impeller formed with a second hole that is obliquely penetrated radially from the inner periphery to the outer periphery, Oxygen discharged from the first hole is discharged to the outside of the impeller through the second hole Stirring device for microbial culture that can supply oxygen. delete According to claim 1, wherein the first hole and the second hole agitation apparatus for microbial culture capable of supplying oxygen, characterized in that a plurality. The apparatus of claim 1, wherein the impeller is supported by a snap ring coupled to the outer periphery of the cylinder block outside the impeller so that the impeller does not escape from the cylinder block. In the stirring device of the microbial culture solution contained in the stirring tank, An oxygen injection tube, one end of which is coupled to an external oxygen tank and the other end of which is immersed in the microbial culture, for supplying oxygen to the microbial culture; A cylinder block coupled to the other end of the oxygen injection pipe, penetrating from the inner circumference to the outer circumference, and having a hole for discharging oxygen to the outside; Located in the periphery of the cylinder block formed with the hole, a propeller is provided on the outer periphery, the inner periphery includes an impeller extending diagonally from the top to the bottom and having a groove made of floors and valleys, Oxygen discharged from the hole is discharged to the outside of the impeller along the groove Stirring device for microbial culture that can supply oxygen. delete The method of claim 1, A foam breaking block coupled to the stopper of the stirring tank and having a bubble breaking hole for passing the foam generated from the stirring tank and applying a compressive force greater than the surface tension of the foam; It is coupled to the inner circumference of the foam breaking block, extending the tube for supplying oxygen through the foam breaking block, and includes an air discharge block formed with a discharge port for discharging the gas generated as the bubble is broken, The bubble breaking hole has a conical taper shape that narrows from the outer circumference to the inner circumference of the foam breaking block so that the pressure increases with respect to the foam passing therethrough. Stirring device for microbial culture that can supply oxygen. delete 8. The oxygen supply method according to claim 7, wherein the space between the inner circumference of the foam breaking block and the outer circumference of the air discharge block is formed such that a compressive force is applied to the foam passing through the foam breaking hole. Stirring device for possible microbial culture. The apparatus of claim 7, wherein a hollow part is formed at a lower portion of the air discharge block, and the outlet port communicates with the hollow part. The apparatus of claim 7, wherein the upper portion of the outlet port has a tapered first groove extending to an outer circumference of the air discharge block. 12. The apparatus of claim 11, wherein a second groove is formed at an end of the first groove to accommodate an antifoaming agent.

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