KR100592972B1 - Sequence characterized amplified region marker for identifying a dominant gene linked to lateral bud growth in Brassica campestris spp. - Google Patents

Sequence characterized amplified region marker for identifying a dominant gene linked to lateral bud growth in Brassica campestris spp. Download PDF

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KR100592972B1
KR100592972B1 KR1020030060738A KR20030060738A KR100592972B1 KR 100592972 B1 KR100592972 B1 KR 100592972B1 KR 1020030060738 A KR1020030060738 A KR 1020030060738A KR 20030060738 A KR20030060738 A KR 20030060738A KR 100592972 B1 KR100592972 B1 KR 100592972B1
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최우진
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Abstract

본 발명은 배추종에서 측아생장유전자를 검정하기 위한 SCAR 표지인자에 관한 것으로, 본 발명은 배추종에서 측아생장우성유전자인 Lb1 및 Lb2를 검정할 수 있는 RAPD 분석을 통해 얻은 SCAR 표지인자를 제공하는 뛰어난 효과가 있다.The present invention relates to SCAR markers for assaying side growth genes in Chinese cabbage, and the present invention relates to SCAR markers obtained through RAPD analysis capable of assaying Lb 1 and Lb 2 , which are lateral growth dominant genes in Chinese cabbage There is an excellent effect to provide.

배추, 측아생장인자, SCAR, 표지인자, RAPD 분석Chinese cabbage, side growth factor, SCAR, marker, RAPD analysis

Description

배추종에서 측아생장우성유전자를 검정하기 위한 ＳＣＡＲ 표지인자 {Sequence characterized amplified region marker for identifying a dominant gene linked to lateral bud growth in Brassica campestris spp.} Sequence characterized amplified region marker for identifying a dominant gene linked to lateral bud growth in Brassica campestris spp.             

도 1은 배추종의 여러 아종을 나타내는 사진도이다.1 is a photograph showing various subspecies of Chinese cabbage.

도 2는 한국 순무의 측아 생장 모습을 나타내는 사진도이다.Figure 2 is a photograph showing the appearance of germ growth of Korean turnips.

도 3은 배추종에 있어서 측아생장유전자와 연관된 DNA 단편의 4개 프라이머별 밴드 양상을 나타낸 젤 사진도이다.Figure 3 is a gel photograph showing the band pattern of the four primers of the DNA fragments associated with the side growth gene in Chinese cabbage.

도 4는 배추종에 있어서 SCAR 프라이머에 의한 측아생장유전자의 증폭결과를 나타낸 젤 사진도이다.Figure 4 is a gel photograph showing the result of amplification of the side growth gene by SCAR primer in Chinese cabbage.

도 5는 측아 무생장계통(8개)과 생장계통(27개)에서 RAPD 프라이머(J-05, AA-20 및 A-02)별 특이밴드의 발현 양상을 나타낸 젤 사진도이다.5 is a gel photograph showing the expression patterns of specific bands for RAPD primers (J-05, AA-20 and A-02) in the lateral germ line growth system (8) and the growth system (27).

도 6은 측아 무생장계통(8개)과 생장계통(27개)에서 SCAR 표지인자(SJ-05, SAA-20 및 SA-02)에 의한 특이밴드의 발현 양상을 나타낸 젤 사진도이다.Figure 6 is a gel photograph showing the expression of the specific bands by SCAR markers (SJ-05, SAA-20 and SA-02) in the lateral germ-free growth system (8) and growth system (27).

도 7은 RAPD 검정이 안된 13개의 측아 생장계통의 SCAR 표지인자에 의한 검정결과를 나타낸 젤 사진도이다.Figure 7 is a gel photograph showing the results of the assay by the SCAR markers of 13 lateral growth system not RAPD assay.

도 8은 측아 생장계통 146번, 원예연구소, 원예 211(미즈나), 원예 220(순 무), 원예 226, 배추 무측지 및 배추 측지계에 대한 본 발명 SCAR 표지인자에 의한 검정결과를 나타낸 젤 사진도이다.8 is a gel photograph showing the assay results of the present invention SCAR markers for the lateral growth system No. 146, Horticultural Research Institute, Horticulture 211 (Mizuna), Horticulture 220 (turnip), Horticulture 226, Chinese cabbage geodetic and Chinese cabbage geodetic system to be.

본 발명은 배추종에서 측아생장유전자를 검정하기 위한 SCAR 표지인자에 관한 것이다. 보다 상세하게는, 본 발명은 배추종에서 측아생장우성유전자인 Lb1 및 Lb2를 검정할 수 있는 RAPD 분석을 통해 얻은 SCAR(Sequence Characterized Amplified Region, 서열 특성화 증폭 부위) 표지인자에 관한 것이다.The present invention relates to SCAR markers for assaying side growth genes in Chinese cabbage. More specifically, the present invention relates to SCAR (Sequence Characterized Amplified Region) markers obtained through RAPD analysis capable of assaying Lb 1 and Lb 2 , which are lateral growth dominant genes in Chinese cabbage.

배추종(Brassica campestris spp.)에는 결구배추, 즉 우리나라의 일반적인 배추(ssp. pekinensis)을 비롯하여 팍쵸이(ssp. chinensis), 순무(ssp. rapa), 다아사이(ssp. narinosa), 임생채(ssp. japonica) 및 ssp parachinensis(한국이름 없음)등의 아종(sub-species)이 분화되어 있다(도 1). Brassica campestris spp. Includes cabbage cabbage, that is, Korean common cabbage (ssp. Pekinensis ), as well as ssp. Chinensis , turnip (ssp. Rapa ), daasai (ssp. Narinosa ) and forest vegetables ( subspecies, such as ssp. japonica ) and ssp parachinensis (no Korean name), are differentiated (FIG. 1).

그들 중 우리나라에서 재배되고 있는 것은 결구배추, 팍쵸이, 순무 등인데 특히 순무는 강화도 지방의 재래종으로서 수백 년 동안 특산물로 재배되어 왔고 일본의 순무와는 뿌리의 크기, 경도(hardness), 조직감(texture)등에서 다르다. 그런데 이 순무는 자라는 동안에 측아가 나와서 여러 개의 순이 되고 수확기에 이들을 따내면 그 흔적이 뿌리 윗부분에 남아 있어 외관상 품질이 크게 떨어지는 문제점을 가지고 있다(도 2). 이처럼 측아가 자라는 것 중에는 임생채가 대표적이며 우리나라 배추는 특별히 재배조건(환경)이 나쁠 때 측아가 생겨 판매를 못하게 되는 경우가 가끔 있었다.Among them, cultivated cabbage, Pakchoi, and turnip are grown in Korea. Especially, turnip is a native species of Ganghwado province and has been grown as a special product for hundreds of years, and Japanese turnip is the size, hardness and texture of roots. It is different from). By the way, this turnip has a problem that the side sprouts come out during the growing season to become a number of sprouts, and when harvesting them, the traces remain in the upper part of the roots so that the appearance quality is greatly reduced (Fig. 2). Among them, the side shoots are representative of forest fresh vegetables, and Korean cabbage is sometimes sold out because of the side conditions, especially when the growing conditions (environment) are bad.

이러한 측아가 영양 생장 기간 중에 자라는 원인을 이해하기 위하여 순무와 배추를 교잡하고 그 후대의 분리 양상을 조사하였으며, 그 결과 측아가 자라는 성질은 2개의 중복 우성 유전자 Lb1 및 Lb2 때문임이 밝혀졌다.To understand the causes of these embryos growing during nutrient growth, turnips and cabbages were hybridized and their later separations were investigated. As a result, the larvae grew because of two overlapping dominant genes, Lb 1 and Lb 2 .

따라서, 본 발명의 목적은 결구배추의 우량품종 육종 시 발생하는 측아 생장 현상의 원인을 손쉽게 제거하기 위한 측아생장에 관여하는 중복우성유전자 Lb1 및 Lb2 에 연관된 서열 특성화 증폭 부위(Sequence Characterized Amplified Region, 이하 SCAR라 함)의 표지 인자를 개발하는 것이다.Accordingly, an object of the present invention is a sequence characterized amplified region associated with the overlapping dominant genes Lb 1 and Lb 2 involved in lateral growth to easily eliminate the cause of lateral growth occurring during the breeding of superior varieties of cabbage cabbage. , Hereinafter referred to as SCAR).

본 발명의 상기 목적은 측아가 자라지 않는 배추와 측아가 여러 개 자라는 순무를 교잡하여 잡종을 만들고, 상기 잡종의 소포자를 배양하여 반수성 2배체의 순계를 육성한 다음, 이들 중 측아가 여러 개 보이는 계통과 측아가 없는 계통을 선택하여 측아 생장군과 측아 무생장군으로 나누고, 이 2개 군을 bulked segregant analysis법으로 10mer random primer를 이용하여 RAPD(Random Amplified Polymorphic DNA) 분석을 실시하여 배추종의 측아생장유전자 Lb1과 Lb2 유전자를 검정할 수 있는 SCAR 표지인자를 얻음으로써 달성하였다. The object of the present invention is to hybridize the napa cabbage and the turnips of several side sprouts do not grow to grow hybrids, to cultivate the vesicles of the hybrids to cultivate the water system of semi-aploid diploid, then several of these strains The two groups were selected into a side growth group and a side growth without group.The two groups were subjected to random amplified polymorphic DNA (RAPD) analysis using a 10mer random primer by bulked segregant analysis. This was achieved by obtaining SCAR markers capable of assaying the genes Lb 1 and Lb 2 genes.

이하, 발명의 구성을 구체적으로 설명한다.
EMBODIMENT OF THE INVENTION Hereinafter, the structure of this invention is demonstrated concretely.

본 발명은 측아가 자라지 않는 배추와 측아가 여러 개 자라는 순무를 교잡하여 잡종을 형성하는 단계; 상기 잡종의 소포자를 배양하여 반수성 2배체의 순계를 육성하는 단계; 상기로부터 얻은 순계를 측아 생장군과 측아 무생장군으로 나누고 bulked segregant analysis법으로 10mer random primer를 이용하여 RAPD(Random Amplified Polymorphic DNA) 분석을 실시하는 단계; 상기 결과로부터 배추종의 측아생장유전자 Lb1과 Lb2 유전자를 검정할 수 있는 SCAR 표지인자를 얻는 단계로 구성된다.The present invention comprises the steps of hybridizing the cabbage and the turnip of the side sprouts do not grow the side sprouts to form a hybrid; Culturing the hybrid vesicles to cultivate a pure system of haploid diploids; Dividing the pure system obtained from the above into a side growth group and a side growth group and performing a random amplified polymorphic DNA (RAPD) analysis using a 10mer random primer by a bulked segregant analysis method; From the above results, it is composed of obtaining SCAR markers capable of assaying the side growth genes Lb 1 and Lb 2 genes of Chinese cabbage.

이하, 본 발명의 구체적인 방법을 실시예를 들어 상세히 설명하고자 하지만 본 발명의 권리범위는 이들 실시예에만 한정되는 것은 아니다.Hereinafter, the specific method of the present invention will be described in detail with reference to Examples, but the scope of the present invention is not limited only to these Examples.

[실시예]EXAMPLE

실시예 1: 배추종에서 측아생장유전자를 검정할 수 있는 SCAR 표지인자의 개발Example 1: Development of SCAR Markers to Test Side Embryonic Genes in Chinese Cabbage

본 발명의 배추종에서 측아생장유전자를 검정할 수 있는 SCAR 표지인자의 개발을 위해, 측아가 자라지 않는 배추와 측아가 여러 개 자라는 순무를 교잡하여 잡종을 만들고, 상기 잡종의 소포자를 배양하여 200 계통 이상의 순계(반수성 2배체)를 육성한 다음, 이들 중 측아가 여러 개 자라는 계통 16개와 측아가 전혀 보이지 않는 계통 8개를 선택하여 측아 생장군과 측아 무생장군으로 나누었다. 상기 2개 군을 bulked segregant analysis법으로 10mer random primer를 이용하여 RAPD(Random Amplified Polymorphic DNA) 분석을 실시하였다.For the development of SCAR markers capable of assaying the side growth genes in the cabbage of the present invention, hybridization is made by hybridizing turnips with several cabbages that do not grow sideways, and cultures the vesicles of the hybrid 200 strains. After growing the above-mentioned pure system (semiploid diploid), 16 strains with multiple flanks and 8 strains without visible flares were selected and divided into lateral growth and lateral growth. The two groups were subjected to random amplified polymorphic DNA (RAPD) analysis using a 10mer random primer by bulked segregant analysis.

그 결과, 도 3에 나타난 바와 같이, 4개의 primer가 측아 생장 유전자와 연관된 DNA 단편(유전자)을 증폭시키는 것으로 나타났다. 이중 SJ-05, SAA-20 및 SA-02의 시퀀스는 다음과 같다:As a result, as shown in Figure 3, four primers were shown to amplify the DNA fragment (gene) associated with the germline growth gene. The sequence of double SJ-05, SAA-20 and SA-02 is as follows:

SJ-05: upper sequence: 5'-GTGTGGGACTAAGTTTTGGGATGG-3'SJ-05: upper sequence: 5'-GTGTGGGACTAAGTTTTGGGATGG-3 '

lower sequence: 5'-TTGCAGAAGAGGGGGAAAGAAAAT-3'       lower sequence: 5'-TTGCAGAAGAGGGGGAAAGAAAAT-3 '

SAA-20: upper sequence: 5'-AATACAATAGCTACAAAAGCTGGC-3'SAA-20: upper sequence: 5'-AATACAATAGCTACAAAAGCTGGC-3 '

lower sequence: 5'-CTAGGTATTAATCTTATCATTTGG-3'        lower sequence: 5'-CTAGGTATTAATCTTATCATTTGG-3 '

SA-02: upper sequence: 5'-ACATGGATGGGCTCACTTACTGAC-3'SA-02: upper sequence: 5'-ACATGGATGGGCTCACTTACTGAC-3 '

lower sequence: 5'-TCCTCTATGGTTGCACTAGCTTCT-3'       lower sequence: 5'-TCCTCTATGGTTGCACTAGCTTCT-3 '

상기의 4개 프라이머로 측아 생장군의 16계통과 그 외 측아 생장 계통 11개를 합한 27계통에 대하여 계통별 검정을 실시하였고 그 결과를 표 1에 나타내었다.As a result of the four primers, 27 lines including 16 lines of the lateral growth group and 11 other lines of the lateral growth line were tested for each line, and the results are shown in Table 1.

측아생장 계통 27개의 선발된 4개 RAPD 프라이머별 특이밴드 유무 구분27 Segmented Growth Lines 프라이머primer 계통번호System number 분리비Separation ratio 특이밴드 유Singular band 특이밴드 무No Singular Band J-05J-05 9, 16, 19, 20, 28, 31, 39, 48, 54, 70, 77, 144, 145, 148, 149, 161, 163 (17)9, 16, 19, 20, 28, 31, 39, 48, 54, 70, 77, 144, 145, 148, 149, 161, 163 (17) 23, 35, 42, 58, 65, 74, 89, 100, 123, 130 (10)23, 35, 42, 58, 65, 74, 89, 100, 123, 130 (10) 17 : 10 (2 : 1)17:10 (2: 1) AA-09AA-09 9, 16, 19, 20, 28, 31, 39, 48, 54, 70, 77, 144, 145, 148, 149, 161, 163 (17)9, 16, 19, 20, 28, 31, 39, 48, 54, 70, 77, 144, 145, 148, 149, 161, 163 (17) 23, 35, 42, 58, 65, 74, 89, 100, 123, 130 (10)23, 35, 42, 58, 65, 74, 89, 100, 123, 130 (10) 17 : 10 (2 : 1)17:10 (2: 1) AA-20AA-20 9, 16, 19, 20, 28, 31, 39, 48, 54, 70, 77, 144, 145, 148, 149, 161, 163 (17)9, 16, 19, 20, 28, 31, 39, 48, 54, 70, 77, 144, 145, 148, 149, 161, 163 (17) 23, 35, 42, 58, 65, 74, 89, 100, 123, 130 (10)23, 35, 42, 58, 65, 74, 89, 100, 123, 130 (10) 17 : 10 (2 : 1)17:10 (2: 1) A-02A-02 9, 16, 23, 28, 31, 35, 39, 42, 48, 54, 58, 65, 70, 74, 89, 100, 130, 145, 148, 149, 161 (21)9, 16, 23, 28, 31, 35, 39, 42, 48, 54, 58, 65, 70, 74, 89, 100, 130, 145, 148, 149, 161 (21) 19, 20, 77, 123, 144, 163 (6)19, 20, 77, 123, 144, 163 (6) 21 : 6 (2 : 1)21: 6 (2: 1)

표 1에 나타난 바와 같이, 3개의 프라이머 J-05, AA-09, AA-20이 나타내는 특이 밴드는 계통별로 동일하였고 남은 1개의 프라이머 A-02가 나타낸 계통별 특이 밴드는 이들 3개 프라이머와 달랐다. 즉, 4개의 프라이머를 A(J-05, AA-09, AA-20)와 B(A-02)의 두 가지로 나눌 수 있었다. 여기서 A와 B 프라이머 모두에서 특이 밴드를 나타낸 계통은 Lb1과 Lb2유전자 모두를 가지고 있고, A에서만 특이 밴드를 나타낸 계통은 Lb1 유전자만, 그리고 B 프라이머에서만 특이 밴드를 나타낸 계통은 Lb2 유전자만 가지고 있다고 가정할 수 있다. 이러한 가정에 따라 27개의 계통을 측아 생장 유전자형 별로 나눈 것이 표 2이다. As shown in Table 1, the specific bands represented by the three primers J-05, AA-09, and AA-20 were identical by lineage, and the line-specific bands represented by the remaining one primer A-02 were different from these three primers. . That is, four primers could be divided into A (J-05, AA-09, AA-20) and B (A-02). Here, the strains showing specific bands in both A and B primers have both Lb 1 and Lb 2 genes, the strains showing specific bands in A only Lb 1 gene, and the strains showing specific bands in B primer only Lb 2 gene. Can only be assumed to have. Based on these assumptions, Table 2 shows the 27 lines divided by the genotyping genotype.

측아생장 계통 27개의 측아생장 유전자 형별 분류Segmented Growth Lines 27 Segmented Growth Genotypes 유전자 형별 계통 번호Genotype line number x2-값x 2 -value Lb1Lb1 Lb2Lb2 Lb 1 Lb 1 Lb 2 Lb 2 Lb1Lb1 lb2lb2 Lb 1 Lb 1 lb 2 lb 2 lb1lb1 Lb2Lb2lb1lb1 Lb2Lb2 9, 16, 28, 31, 39, 48, 54, 70, 145, 148, 149, 161 (12)9, 16, 28, 31, 39, 48, 54, 70, 145, 148, 149, 161 (12) 19, 20, 77, 144, 163 (5)19, 20, 77, 144, 163 (5) 23, 35, 42, 58, 65, 74, 89, 100, 130 (9)23, 35, 42, 58, 65, 74, 89, 100, 130 (9) 1.75NS1.75NS

상기의 4개의 10mer 프라이머를 20-24mer 프라이머(SCAR primer)로 전환하여 상기의 측아 무생장군과 측아 생장군의 2개 군에 대하여 검정하였다. The four 10mer primers were converted into 20-24mer primers (SCAR primers) and assayed for the two groups, the lateral growth group and the lateral growth group.

그 결과, 도 4에 나타난 바와 같이, 10mer 프라이머의 결과와 마찬가지로 측아 생장군에서만 특이 밴드가 나타났으며, 한편 10mer 프라이머의 결과와 다른 특이 밴드는 하나도 나타나지 않았다. As a result, as shown in Figure 4, as in the result of the 10mer primer, the specific band appeared only in the lateral growth group, while no other specific band and the result of the 10mer primer.

또한, 이 4개의 SCAR 프라이머로 상기에서와 같은 27계통에 대하여 계통 검정을 실시하였다.In addition, these four SCAR primers were subjected to a lineage assay for the 27 lines as described above.

도 5 내지 8에 나타난 바와 같이, SAA-9은 모든 계통에서 전혀 특이 밴드가 나타나지 않아 프라이머가 잘못 제조된 것으로 간주하였다. 그 외 3개는 각기 특이 밴드를 보였는데 SJ-05는 10mer의 결과와는 다르게 Lb1과 Lb2 유전자 모두의 연관 유전자를 증폭하였고, SAA-20은 Lb1 유전자만, 그리고 SA-02는 Lb2 유전자에 연관된 유전자만을 증폭하였다. 각기 다른 3개의 SCAR 프라이머로 지금까지 검정하지 않았던 측아 생장 계통 13계통에 대하여 검정한 결과 1계통(계통번호 146)만 3개 프라이머 모두에서 특이 밴드가 나타나지 않았고 남은 12계통은 특이 밴드를 모두 나타내었다(도 7). 그리고 이들을 측아 생장 유전자형별로 나누어 보면 두개의 우성 유전자 Lb1과 Lb2를 모두 가진 것이 3계통, Lb2 유전자만 가진 것이 9계통이었고 Lb1 유전자만 가진 것은 없는 것으로 나타났다.As shown in Figs. 5 to 8, SAA-9 did not show any specific band in all lines, so it was considered that the primer was incorrectly prepared. The other three showed specific bands. SJ-05 amplified the associated genes of both Lb 1 and Lb 2 genes, unlike the results of 10mer, SAA-20 only Lb 1 gene, and SA-02 Lb. Only the genes associated with the 2 genes were amplified. As a result of testing for 13 strains of the lateral growth lines that have not been tested with three different SCAR primers, only one strain (line No. 146) showed no specific band in all three primers, and the remaining 12 strains showed all the specific bands. (FIG. 7). Dividing them by genotyping genotype showed that two dominant genes, Lb 1 and Lb 2 , had three lines, and only Lb 2 had 9 lines and none had Lb 1 gene.

상기의 결과로부터 본 발명자는 배추종의 측아 생장유전자 Lb1과 Lb2 를 검정하는데 있어 SCAR 표지인자로 SS-05, SAA-20 및 SA-02를 얻을 수 있었다. From the above results, the present inventors were able to obtain SS-05, SAA-20 and SA-02 as SCAR markers in assaying the germline growth genes Lb 1 and Lb 2 of Chinese cabbage.

상기 실시예를 통해 살펴본 바와 같이, 본 발명은 배추종에서 측아생장유전자를 검정하기 위한 SCAR 표지인자에 관한 것으로, 배추종에서 측아생장우성유전자인 Lb1 및 Lb2를 검정할 수 있는 RAPD 분석을 통해 얻은 SCAR 표지인자를 제공하는 뛰어난 효과가 있다. 따라서, 본 발명은 상기의 SCAR 표지인자를 측아가 자라지 않는 품종의 육성에 사용할 수 있어 신품종 육성산업상 매우 유용한 발명인 것이다.As described through the above examples, the present invention relates to a SCAR marker for assaying the side growth genes in Chinese cabbage, and to analyze the RAPD analysis capable of assaying the Lb 1 and Lb 2 of the side growth dominant genes in Chinese cabbage There is an excellent effect of providing the SCAR markers obtained. Therefore, the present invention can be used for the breeding of the above-mentioned SCAR markers do not grow the side is a very useful invention in the new breed breeding industry.

<110> LEE, Soo-Sung CHOI, Wo-Jeen <120> SCAR marker for identifying a dominant gene linked to lateral bud growth in Brassica campestris spp. <160> 6 <170> KopatentIn 1.71 <210> 1 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Upper sequence of SCAR marker SJ-05 <400> 1 gtgtgggact aagttttggg atgg 24 <210> 2 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Lower sequence of SCAR marker SJ-05 <400> 2 ttgcagaaga gggggaaaga aaat 24 <210> 3 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Upper sequence of SCAR marker SAA-20 <400> 3 aatacaatag ctacaaaagc tggc 24 <210> 4 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Lower sequence of SCAR marker SAA-20 <400> 4 ctaggtatta atcttatcat ttgg 24 <210> 5 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Upper sequence of SCAR marker SA-02 <400> 5 acatggatgg gctcacttac tgac 24 <210> 6 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Lower sequence of SCAR marker SA-02 <400> 6 tcctctatgg ttgcactagc ttct 24 <110> LEE, Soo-Sung          CHOI, Wo-Jeen <120> SCAR marker for identifying a dominant gene linked to lateral bud          growth in Brassica campestris spp. <160> 6 <170> KopatentIn 1.71 <210> 1 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Upper sequence of SCAR marker SJ-05 <400> 1 gtgtgggact aagttttggg atgg 24 <210> 2 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Lower sequence of SCAR marker SJ-05 <400> 2 ttgcagaaga gggggaaaga aaat 24 <210> 3 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Upper sequence of SCAR marker SAA-20 <400> 3 aatacaatag ctacaaaagc tggc 24 <210> 4 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Lower sequence of SCAR marker SAA-20 <400> 4 ctaggtatta atcttatcat ttgg 24 <210> 5 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Upper sequence of SCAR marker SA-02 <400> 5 acatggatgg gctcacttac tgac 24 <210> 6 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Lower sequence of SCAR marker SA-02 <400> 6 tcctctatgg ttgcactagc ttct 24

Claims (3)

서열목록 서열번호 1과 2에 기재된 배추종의 측아생장우성유전자 Lb1과 Lb2 유전자를 검정할 수 있는 SCAR(Sequence Characterized Amplified Region) 표지인자 SJ-05의 염기서열. Nucleotide sequence of the SCAR (Sequence Characterized Amplified Region) marker SJ-05 capable of assaying the side germline dominant genes Lb 1 and Lb 2 genes of the cabbage species shown in SEQ ID NOs: 1 and 2. 서열목록 서열번호 3과 4에 기재된 배추종의 측아생장우성유전자 Lb1 유전자를 검정할 수 있는 SCAR 표지인자 SAA-20의 염기서열.Nucleotide sequence of the SCAR marker SAA-20 capable of assaying the side growth dominant gene Lb 1 gene of the cabbage species described in SEQ ID NOs: 3 and 4. 서열목록 서열번호 5와 6에 기재된 배추종의 측아생장우성유전자 Lb2 유전자를 검정할 수 있는 SCAR 표지인자 SA-02의 염기서열.Nucleotide sequence of the SCAR marker SA-02 capable of assaying the sideloid dominant Lb 2 gene of the cabbage species described in SEQ ID NOs: 5 and 6.
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