KR100560991B1 - Multi-protein analyzing apparatus - Google Patents

Abstract

The present invention relates to a multiple crude protein analysis device capable of continuously performing the entire analysis work without a replacement in the middle by distilling and titrating sequentially by plural samples at the same time, the first and second decomposition samples are respectively First and second reaction vessels 20 and 30 contained therein; A distilled water supply device 40 for supplying distilled water to the first and second reaction vessels 20 and 30; A sodium hydroxide supply device 50 for supplying sodium hydroxide to the first and second reaction vessels 20 and 30; A steam supply device 60 for supplying steam to the first and second reaction vessels 20 and 30; A condenser (70) having first and second cooling tubes connected to the first and second reaction vessels (20, 30); First and second cooling tube valves 81 and 82 connected between the first and second reaction vessels 20 and 30 and the first and second cooling tubes 71 and 72 of the condenser 70; It is composed of a titration device 90 for receiving and titrating the ammonia liquid discharged from the condenser 70, by placing a plurality of samples at a time in the distillation apparatus and distilling them sequentially with the condenser 70, without a large number of replacement work in the middle Samples can be titrated continuously, the analysis time can be shortened by omitting such replacement work, and other work can be performed in parallel during the analysis time.

Kjeldahl Method, Crude Protein Analysis, Cooling Tube, Condenser, Alkaline Solution, Distilled Water

Description

Multi-Protein Analyzing Apparatus             

1 is a Kjeldahl decomposition apparatus applied to the present invention,

Figure 2 is a block diagram showing an embodiment of a crude protein analysis apparatus according to the present invention,

3 is a connection diagram showing a connection state with a condenser and a reaction vessel having a double cooling tube according to the present invention;

Figure 4 is a block diagram showing another embodiment of a multiple crude protein analysis apparatus according to the present invention,

5 is a cross-sectional view showing the interior of a condenser having multiple cooling tubes according to the present invention.

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♠ Explanation of symbols for the main parts of the drawing ♠

10: Kjeldahl cracking device 11: Kjeldahl flask

20, 30: first and second reaction vessel 21, 31: first and second decomposition sample solution

40: distilled water supply device 41: distilled water tank

42, 43: first and second distilled water pump 50: alkali supply device

51: alkali storage container 52, 53: first and second alkali supply device

60: steam supply device 61: steam generator

62,63: first and second steam valve 70: condenser

81, 82: first and second cooling tube valve 90: titrator

The present invention relates to a multiple crude protein analysis apparatus, and more particularly, to a multiple crude protein analysis apparatus capable of continuously performing the entire analysis without replacing the intermediate work by distilling and titrating sequentially by a plurality of samples at the same time.

Crude protein analysis device is a device for measuring the protein content in the sample, not the protein content directly, but the nitrogen content in the protein component. Since the nitrogen content is generally included according to the type of food, the nitrogen content is measured and converted into the amount of protein.

The crude protein analysis device is composed of a decomposition device, a distillation device and a titration device. The decomposition device decomposes a food sample into a strong acid (for example, concentrated sulfuric acid) at a temperature of about 460 degrees to form a nitrogen compound.

When strong alkali is added to the nitrogen compound in a distillation apparatus to neutralize the remaining acid, the nitrogen is vaporized by slight heating and evaporated. After condensation in the condensation tube, the amount of nitrogen is analyzed by titration in a titrator. do.

In general, distillation and automatic titration are carried out automatically with one instrument, and samples are measured in large quantities of 2 to 4 or more. Conventional analyzers take 20-30 minutes to distill one sample at a time, and the sample must be replaced to distill the next sample, so the operator watches the distillation apparatus and distills one sample. After that, the next sample had to be connected to the distillation unit, so it was difficult to leave the analyzer and do anything else.

Accordingly, the present invention has been made to solve various problems as described above, and an object of the present invention is to provide a multiple crude protein analysis apparatus that can be titrated by sequentially distilling a plurality of samples in a distillation apparatus.

It is another object of the present invention to provide a multiple crude protein analysis apparatus capable of shortening the analysis time by installing a plurality of samples in a distillation apparatus, distillation, and titration, thereby eliminating the need for intermediate replacement work.

Another object of the present invention is a crude protein analysis device by a condenser equipped with a double cooling tube that can be performed in parallel with another operation by distillation apparatus and a plurality of samples in the distillation apparatus and sequentially distillation and do not need to replace the sample during the distillation time To provide.

Crude protein analysis device according to the present invention for achieving the above object comprises a first and second reaction vessels each containing a first decomposition sample and a second decomposition sample; First and second distilled water supply devices for supplying distilled water to the first and second reaction vessels; First and second alkali supply devices for supplying alkalis to the first and second reaction vessels; First and second steam supply devices for supplying steam to the first and second reaction vessels; A condenser having first and second cooling tubes connected to the first and second reaction vessels; First and second cooling tube valves connected between the first and second reaction vessels and the first and second cooling tubes of the condenser; It consists of a titration device for receiving and titrating the ammonia liquid discharged from the condenser.

The condenser has a first cooling tube and a second cooling tube spirally arranged side by side in two rows, the two ends are connected to the lower end to form a spherical portion, the spherical portion is characterized in that it leads to the condensate discharge pipe.

Crude protein analysis device according to another embodiment of the present invention comprises a plurality of reaction vessels each containing a plurality of decomposition samples; A plurality of distilled water pumps respectively supplying distilled water to the plurality of reaction vessels; A plurality of alkali supply devices for supplying an alkaline liquid to the plurality of reaction vessels; A plurality of steam valves respectively supplying steam to the plurality of reaction vessels; A condenser connected to the plurality of reaction vessels; A plurality of cooling tube valves connected between the plurality of reaction vessels and the condenser; It consists of a titration device for receiving and titrating the ammonia liquid discharged from the condenser.

The condenser is connected to each of the plurality of reaction vessels, each side is formed in a spiral side by side in two rows downward, the whole is composed of a plurality of cooling pipes extending from the lower end to form a communication portion, the communication portion is connected to the condensate discharge pipe It is characterized by.

Hereinafter, with reference to the accompanying drawings in accordance with embodiments of the present invention will be described in more detail the technical configuration of the present invention.

Figure 1 shows a Kjeldahl cracking apparatus applied to the present invention.

Accurately weigh 1 to 5 g of a number of food samples (e.g. flour, pork, etc.) into a number of Kjeldahl flasks 11, add a decomposition promoter, 10 ml of concentrated sulfuric acid, and slowly in the decomposition table 10. Heat 30 minutes.

At this time, as the reaction proceeds, the carbon component becomes CO 2 and scatters, and the liquid becomes transparent and becomes pale green. After cooling to room temperature for 10-15 minutes, the color of the liquid changes from light green to white.

Carefully add 20 ml of distilled water to prevent precipitation of salt crystals, add the decomposition solution to the reaction vessel (for example, 100 ml volumetric flask, etc.) and add distilled water to a standard volume (for example, 100 ml). Dilute the digestion solution.

Many food sample decomposition liquids obtained in this decomposition process are titrated by the following process by the distillation apparatus and titration apparatus according to the present invention.

Figure 2 is a block diagram showing an embodiment of a crude protein analysis apparatus according to the present invention.

The crude protein analysis apparatus according to the present invention includes first and second reaction vessels 20 and 30 each containing a first decomposition sample and a second decomposition sample; A distilled water supply device 40 for supplying distilled water to the first and second reaction vessels 20 and 30; An alkali supply device (50) for supplying alkali to the first and second reaction vessels (20, 30); A steam supply device 60 for supplying steam to the first and second reaction vessels 20 and 30; A condenser (70) having first and second cooling tubes connected to the first and second reaction vessels (20, 30); First and second cooling tube valves 81 and 82 connected between the first and second reaction vessels 20 and 30 and the first and second cooling tubes 71 and 72 of the condenser 70; It consists of a titrator 90 for receiving and titrating the ammonia liquid discharged from the condenser 70.

In the first and second reaction vessels 20 and 30, a first decomposition sample solution 21 and a first decomposition sample obtained by heating the first food sample and the second food sample by mixing strong sulfuric acid by the Kjeldahl digestion apparatus shown in FIG. Dilute sample solution 31 is contained, respectively.

As shown in FIG. 3, the first and second reaction vessels 20 and 30 are sealed by stoppers 22 and 32, and the distilled water supply apparatuses are formed by the first and second distilled water pipes 44 and 45. 40 is connected to the alkali supply device 50 by the first and second alkali pipes 54 and 55, and the steam supply device 60 is connected to the first and second steam pipes 64 and 65. Is connected to.

In addition, the first and second cooling connectors 83 and 84 are connected to the first and second cooling tubes 71 and 72 of the condenser 70, respectively.

The first distilled water pump 42 and the first alkali pump 52 are operated in the first reaction vessel 20 configured as described above to supply distilled water and an alkaline solution (for example, Na OH solution) to obtain a first decomposition sample solution ( 21) is strongly alkaline.

Subsequently, the first distilled water pump 42 and the first alkali pump 52 are stopped, and the first steam valve 62 is opened to supply steam to the first reaction vessel 20. At this time, the steam of about 600 degrees is supplied to the first reaction vessel 20 so that the first decomposition sample solution 21 therein becomes boiling and ammonia is generated.

The ammonia gas thus generated is opened and passed through the first cooling tube valve 81 of the condenser 70 to condense. The distillation is carried out for 15 to 20 minutes and the ammonia water is contained in the receiver 92 (for example, a beaker) of the titrator 90.

As shown in FIG. 3, the condenser 70 according to the present invention spirals downward in parallel with two rows of the first cooling tube 71 and the second cooling tube 72, and the two ends thereof are connected at the lower end thereof. A spherical portion 73 is formed. In addition, the spherical portion 73 is connected to the condensate discharge pipe 74, the condensate passing through the first cooling tube 71 and the second cooling tube 72, respectively, through the spherical portion 73 and the condensate discharge pipe 74 (receptor ( 92).

After containing the ammonia water in the receiver 92, the boric acid pump 93 is operated to supply boric acid through the boric acid supply pipe 94 for titration.

After the crude protein analysis of the first decomposition sample solution 21 is finished, the second distilled water pump 43 and the second alkali pump 53 are operated in the second reaction vessel 30 to supply distilled water and an alkaline solution. The sample solution 31 is made alkaline strongly.

Then, the second distilled water pump 43 and the second alkali pump 53 are stopped, and the second steam valve 63 is opened to supply steam to the second reaction vessel 30. The inside of the second reaction vessel 30 is supplied with steam of about 600 degrees, the second decomposition sample solution 31 therein is a boiling state to generate ammonia.

Subsequently, the second cooling tube valve 82 is opened to pass the ammonia gas of the second reaction vessel 30 through the second cooling tube 72 of the condenser 70 to condense it. Similarly, distillation is carried out for 15 to 20 minutes, ammonia water is contained in the receiver 92 of the titrator 90, and the boric acid pump 93 is operated to supply and titrate boric acid.

Thus, by placing two samples in a distillation apparatus and distilling them sequentially with a condenser provided with a double cooling tube, two samples can be continuously titrated without replacement in the middle.

As a second embodiment, a multiple crude protein analysis apparatus which connects a plurality of reaction vessels and a distilled water supply apparatus through a plurality of valves to a condenser and installs a plurality of samples in a distillation apparatus to be distilled sequentially and titrates in sequence.

Figure 4 shows a block diagram showing another embodiment of the multiple crude protein analysis apparatus according to the present invention.

The multiple crude protein analysis apparatus according to the present embodiment includes a plurality of reaction vessels 20-1, 20-2, ..., 20-n, each containing a plurality of decomposition samples; A plurality of distilled water pumps 40-1, 40-2, ..., 40-n respectively supplying distilled water to the plurality of reaction vessels 20-1, 20-2, ..., 20-n; ; A plurality of alkali supply devices (50-1, 50-2, ..., 50-n) for supplying an alkaline liquid to the plurality of reaction vessels (20-1, 20-2, ..., 20-n) )Wow; And a plurality of steam valves 60-1, 60-2, ..., 60-n for supplying steam to the plurality of reaction vessels 20-1, 20-2, ..., 20-n, respectively. ; A condenser (70) connected to the plurality of reaction vessels (20-1, 20-2, ..., 20-n); A plurality of cooling tube valves (80-1, 80-2, ..., 80) connected between the plurality of reaction vessels (20-1, 20-2, ..., 20-n) and the condenser 70 -n); It consists of a titrator 90 for receiving and titrating the ammonia liquid discharged from the condenser 70.

The plurality of reaction vessels 20-1, 20-2,..., 20-n each contain a plurality of decomposition samples, each sealed by a stopper (see FIG. 3), and a distillation tank 46. Connected to the plurality of distilled water pumps 40-1, 40-2, ..., 40-n, and also to the alkali storage container 56. , 50-n), connected to the steam supply 60 by a plurality of steam valves 60-1, 60-2, ..., 60-n, and also a plurality of cooling pipe valves. (80-1, 80-2, ..., 80-n) to each of the plurality of cooling conduits 74-1, 74-2, ..., 74-n of the condenser 70 by do.

The first distilled water pump 40-1 and the first alkali pump 50 in the first reaction vessel 20-1 among the plurality of reaction vessels 20-1, 20-2,. -1) is operated to supply distilled water and an alkaline solution (eg, Na OH solution) to make the first decomposition sample solution 21 highly alkaline.

Subsequently, the first distilled water pump 40-1 and the first alkali pump 50-1 are stopped, and the first steam valve 60-1 is opened to supply steam to the first reaction vessel 20-1. At this time, about 600 degrees of steam is supplied to the first reaction vessel 20-1, and the first decomposition sample solution 21-1 therein is boiled to generate ammonia.

The ammonia gas thus generated is opened and passed through the first cooling tube 74-1 of the condenser 70 to condense by opening the first cooling tube valve 80-1. After distillation for 15 to 20 minutes, ammonia water is placed in the receiver 92 of the titrator 90. After containing the ammonia water in the receiver 92, the boric acid pump 93 is operated to supply boric acid through the boric acid supply pipe 94 for titration.

The solution after the titration is discharged by the discharge pump (95) to wash the receiver (92) for analysis of the next decomposition sample.

After the crude protein analysis of the first decomposition sample solution 21-1 contained in the first reaction vessel 20-1 is finished, the second reaction vessel 20-2 is performed in the first reaction vessel 20-1. The second distilled water pump 40-2, the second alkali pump 50-2, and the second steam valve 60-2 are operated in the same manner as in the above, to supply distilled water and an alkaline solution, and to supply steam. Ammonia gas is generated from the second decomposition sample solution 21-2, and the ammonia gas is opened by passing the second cooling tube valve 80-2 through the second cooling tube 74-2 of the condenser 70 to cool it. To condense. The condensed ammonia water is contained in the receiver 92 of the titrator 90, titrated with the titrator 90, and discharged by the discharge pump 95 to wash the receiver 92.

From the third reaction vessel 20-3 to the n-th reaction vessel 20-n, crude protein analysis is performed by the same procedure.

Here, as shown in FIG. 5, the condenser 75 according to the present embodiment is connected to the plurality of reaction vessels 20-1, 20-2, ..., 20-n, respectively, and adjacent cooling tubes. It consists of a plurality of cooling tubes (76-1, 76-2, ..., 76-n) spiraling downward in two rows side by side, the whole of which continues from the lower end to form the communication portion (77).

In addition, the communication unit 77 is connected to the condensate discharge pipe 78, the condensate passing through each cooling pipe (76-1, 76-2, ..., 76-n) is the communication unit 77 and the condensate discharge pipe 78 It is discharged to the receiver 92 through.

In the multiple crude protein analysis apparatus according to the present invention, after a plurality of reaction vessels are installed in a distillation apparatus, crude protein analysis is continuously performed without replacing the intermediate reaction from the first reaction vessel 20-1 to the nth reaction vessel 20-n. Can be completed.

As described above, by dividing a plurality of samples at once in a distillation apparatus and distilling them sequentially with a condenser, a plurality of samples can be titrated continuously without replacing the sample in the middle, and the analysis time can be shortened by omitting such replacement work. You can do it and do other things in parallel during the analysis.

Claims (4)

First and second reaction vessels 20 and 30, each containing a first decomposition sample and a second decomposition sample; A distilled water supply device 40 for supplying distilled water to the first and second reaction vessels 20 and 30; An alkali supply device (50) for supplying alkali to the first and second reaction vessels (20, 30); A steam supply device 60 for supplying steam to the first and second reaction vessels 20 and 30; A condenser (70) having first and second cooling tubes (71, 72) connected to the first and second reaction vessels (20, 30); First and second cooling tube valves 81 and 82 connected between the first and second reaction vessels 20 and 30 and the first and second cooling tubes 71 and 72 of the condenser 70; Multiple crude protein analysis device, characterized in that consisting of a titrator (90) for receiving and titrating the ammonia liquid discharged from the condenser (70). According to claim 1, The condenser 70 is the first cooling tube 71 and the second cooling tube 72 is formed in a spiral side by side in two rows in a row, the two ends are connected to the lower end of the spherical portion ( 73), wherein the spherical portion (73) is a multiple crude protein analysis device, characterized in that connected to the condensate discharge pipe (74). A plurality of reaction vessels 20-1, 20-2, ..., 20-n each containing a plurality of decomposition samples; A plurality of distilled water pumps 40-1, 40-2, ..., 40-n respectively supplying distilled water to the plurality of reaction vessels 20-1, 20-2, ..., 20-n; ; A plurality of alkali supply devices (50-1, 50-2, ..., 50-n) for supplying an alkaline liquid to the plurality of reaction vessels (20-1, 20-2, ..., 20-n) )Wow; And a plurality of steam valves 60-1, 60-2, ..., 60-n for supplying steam to the plurality of reaction vessels 20-1, 20-2, ..., 20-n, respectively. ; A condenser (70) connected to the plurality of reaction vessels (20-1, 20-2, ..., 20-n); A plurality of cooling tube valves (80-1, 80-2, ..., 80) connected between the plurality of reaction vessels (20-1, 20-2, ..., 20-n) and the condenser 70 -n); Multiple crude protein analysis device, characterized in that consisting of a titrator (90) for receiving and titrating the ammonia liquid discharged from the condenser (70). According to claim 3, The condenser 70 is connected to each of the plurality of reaction vessels (20-1, 20-2, ..., 20-n), respectively downward in a spiral line in two rows, The whole is composed of a plurality of cooling pipes (76-1, 76-2, ..., 76-n) extending from the lower end forming the communication portion 77, the communication portion 77 is a condensate discharge pipe 78 Multiple crude protein analysis apparatus characterized in that following).

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