KR100536163B1 - Compositions for the prevention or treatment of cancer - Google Patents

Abstract

The present invention is deodorant blowfish against 1L of water; Root skin; Celestial arches; Wolfberry; Earth; And 500-1500 g of jinjin wormwood; 5 to 100 g; 5 to 100 g; 5 to 100 g; 5 to 100 g; And it provides a composition for preventing or treating cancer comprising a water extract obtained by distilling the mixture comprising a ratio of 5 to 100 g and a pharmaceutically acceptable additive.

Description

Compositions for the prevention or treatment of cancer}

The present invention relates to a composition for preventing and treating cancer comprising extracts obtained from detoxified puffer fish, root roots, horoscopes, goji berry, earth worm, and jinjin wormwood.

Cancer means a disease caused by abnormal proliferation of cells. That is, abnormality occurs in the normal cell division regulating function that suppresses unnecessary cell division of normal cells, and the disease is caused by abnormally persistent cell division of cells. When cells multiply abnormally, they form cell populations that invade surrounding tissues and organs, and metastases spread through even the tissues and organs far from the site of development, eventually paralyzing the individual's normal function and causing death. It leads to About 80% of these cancers are recognized to be caused by lifestyle and environmental factors. Research into various chemical, physical, and biological substances that cause cancer is actively conducted, and thus, substances for preventing or treating cancer have been reported.

Blowfish is useful as a diet food containing low calorie, high protein, low fat and various minerals and vitamins, and Korean Patent Publication No. 2002-91641 discloses breast cancer, stomach cancer, lung cancer, liver cancer and ovarian cancer using aqueous extracts of puffer fish eggs. Pharmaceutical compositions capable of treating cancer have been disclosed. However, since such a composition is to be extracted from the puffer eggs, there is a problem in the extraction in large quantities. In addition, the above-described invention focuses on the fact that blowfish poison has a function of inhibiting cell division, thereby preparing an extract containing blowfish poison component. Therefore, the patent only discloses a method for producing an aqueous extract having cancer therapeutic activity from the portion containing blowfish poison, namely, the testes, ovaries, liver, spleen, eyes and blood. Therefore, those skilled in the art will not be able to predict that an aqueous extract having anticancer activity can be obtained even in a portion containing no blowfish poison.

The inventors of the present invention have been researching to develop natural extracts useful for the prevention and treatment of cancer. The present invention was found to be able to effectively inhibit as well as to induce suicide of cancer cells, thereby completing the present invention.

It is therefore an object of the present invention to provide extracts useful for the prevention and treatment of cancer.

According to one aspect of the invention, puffer fish detoxified in 1 liter of water; Root skin; Celestial arches; Wolfberry; Earth; And 500-1,500 g of phosphorus mugwort, respectively; 5 to 100 g; 5 to 100 g; 5 to 100 g; 5 to 100 g; And it provides a composition for preventing or treating cancer comprising water extract and pharmaceutically acceptable additives obtained by distilling the mixture comprising a ratio of 5 to 100g.

The composition of the present invention preferably is blowfish detoxified in 1 L of water; Root skin; Celestial arches; Wolfberry; Earth; And 800 to 1,000 g of phosphorus mugwort, respectively; 10-50 g; 10-50 g; 10-50 g; 10-50 g; And it provides a composition for preventing or treating cancer comprising water extract and pharmaceutically acceptable additives obtained by distilling the mixture comprising a ratio of 10 to 50g.

The puffer fish used in the present invention may use any taxonomically known puffer fish, for example, bokbokdae, firefish. You can use all of the puffer fish belonging to the turtle suit, thorn suit and the like. As a blowfish belonging to the bokbok family, the blowfish inhabiting the waters of Korea are known as 19 species including 18 species such as cock, sword, magpie, and 1 species of thorn. Blowfish are particularly toxic, including black robes, cocks and magpies.

In the present invention, "adventured blowfish" refers to a blowfish that has been removed a neurotoxin called tetradotoxin contained in the liver, gonad, digestive tract, skin, etc. of the blowfish, and usually removes the fins, gills, and eyes of the blowfish Remove pufferfish, puffer fish, and blood and wash in running water for 1 hour to detoxify.

Root root skin is a bark of elm or root and is used for moisturizing, diuretic and small poison. Cheonggung is a perennial herb that is native to China and grows with donkeys in the cold mountainous regions of Korea. The stem roots in the form of lumps in the ground are called celestial arches. Herbal roots in the stomach, bowel, epilepsy, toothache, lobster, analgesic, tonic, soothing, gynecological, hurricane, reversed medicine is used. The earth is the fruit of the broad-leaved broad-leaved arborescent (tree with large and tall leaves) belonging to the sea buckthorn family, and it is a medicinal plant called by other names such as sesame, barn, grass, and volley. Goji berries are fruits of goji berry, which contain protein, fat, sugar, calcium, phosphorus, iron, betaine, rutin, and vitamins (A, B1, B2, C). In oriental medicine, it is used as a tonic and antipyretic. Injin mugwort is a perennial mugwort belonging to the chrysanthemum family, and it is known for treating liver diseases such as jaundice since ancient times, and it is known that the digestive system or anticancer effect can be expected.

The water extract contained in the composition of the present invention is blowfish detoxicated in 1L of water; Root skin; Celestial arches; Wolfberry; Earth; And 500-1,500 g of phosphorus mugwort respectively; 5 to 100 g; 5 to 100 g; 5 to 100 g; 5 to 100 g; And it is obtained by distilling the mixture containing in the ratio of 5-100 g. The distillation method can be carried out according to a conventional distillation method (for example, simple distillation), preferably in a batch distillation column at a temperature of 100 ℃ to 150 ℃, 2 to 7 hours at a pressure of 1 to 5 kgF / cm ² , More preferably, by distilling for 5 to 7 hours at a temperature of 125 ℃ to 135 ℃, pressure 2.8 to 3.2 kgF / cm ² , it is possible to extract useful components effectively.

Since the water extract used in the present invention is distilled from a mixture containing a large amount of deodorized puffer fish in the amount of 500 to 1,500 g per liter of water, it is possible to separate the useful components, particularly proteins and peptides contained in the puffer fish, in liquid and solid phase. Make sure In particular, active ingredients in pufferfish (e.g., useful peptides, widows, new technology fusion projects 2003: see "Discovery, Information and Application of Useful Peptide Ligands") are present in very small quantities and are useful in small amounts of puffer fish. Since it is difficult to obtain the ingredients, it is intended to obtain a highly concentrated active ingredient by using a large amount of blowfish. In addition, the extract of the present invention is extracted from a mixture containing the roots, cheongung, gojija, earthworms, and jinjin mugwort in addition to the admiral blowfish, the cancer prevention or therapeutic effect of the composition of the present invention It seems to be because it works synergistically.

As the pharmaceutically acceptable additive contained in the composition of the present invention, one or two or more of conventional excipients, disintegrants, binders, lubricants, suspending agents, and the like may be selectively used. For example, when the composition of the present invention is prepared in a solid form such as tablets or hard capsules, microcrystalline cellulose, lactose, low-substituted hydroxycellulose and the like can be used as excipients, sodium starch glycolate, anhydrous as a disintegrant. Calcium monohydrogen phosphate may be used. As the binder, polyvinylpyrrolidone, low-substituted hydroxypropyl cellulose, hydroxypropyl cellulose and the like can be used. The lubricant can be selected from magnesium stearate, silicon dioxide, talc and the like. In addition, as the suspending agent, surfactants (eg, sorbitan esters, polysorbates, etc.) commonly used in the pharmaceutical field may be used.

The compositions of the present invention can be formulated in a variety of forms, including solid and liquid forms, which include tablets, capsules (hard or soft), solutions, suspensions, emulsions, syrups and the like. It may preferably have liquid formulations such as liquids, suspensions, emulsions, and syrups, and may also be prepared as a functional food.

The composition of the present invention may be administered by various methods of administration, including oral or parenteral, and may be preferably administered orally. The composition of the present invention can be administered to a person in need of cancer prevention or treatment in the range of 0.1 to 1,000 mg / kg per day. However, the dosage may be adjusted to an appropriate amount depending on the condition such as age, age and gender and the severity of the disease, such dosage can be easily adjusted by those skilled in the art clinically engaged in the treatment or prevention of patients. Can be.

In the present invention, the composition includes one having a prophylactic or therapeutic effect on liver cancer, lung cancer, rectal cancer, stomach cancer, breast cancer, pancreatic cancer, skin cancer, cervical cancer, colon cancer and esophageal cancer, but is not limited thereto. Preferably, it has a prophylactic or therapeutic effect against gastric cancer, colon cancer, liver cancer, cervical cancer, and / or esophageal cancer.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, this embodiment is for illustrative purposes only and the scope of the present invention is not limited to this embodiment.

Example 1

The fins, gills, and eyes of the abalone ( Fugu rubripes rubripes ) were removed, and the abdominal glands, puffer fish, and blood were removed, washed in running water for 1 hour, and detoxified. 30 kg of decontaminated puffer fish was mixed in 20 L of water with 500 g of rhizome skin, 500 g of hemunggi, 500 g of goji berries, 500 g of earthworms, and 500 g of jinjin mugwort. The mixture was injected into a cylindrical batch distillation column (55 L capacity). Next, distillation was performed for about 6 hours at the temperature of 130 degreeC and the pressure of 3 kgF / cm <^2> .

As a result, 30 L of a liquid extract containing blowfish and effective proteins, peptides, and the like of the five kinds of herbal medicines were obtained on the top, and 20 kg of the remaining solid material was obtained at the bottom of the tower.

The liquid extract obtained from the column top was examined for anticancer activity.

Example 2 Evaluation of Anticancer Activity Using TE2, SW480, HepG2, HeLa, SNU-638, SNU-688, and AGS Cells

The blowfish and five kinds of herbal extracts prepared in Example 1 were treated with cancer cells to investigate whether the cancer cells were killed. The cells used in the experiment were TE2 (human esophageal cancer cell line; Cell Resource Center for Biomedical Research, Institute of Development, Aging and Cancer, Tohoku University, Japan; http://www.idac.tohoku.ac.jp/dep/ccr/ ; Catalog # TKG 0253), SW480 (Human Colon Cancer Cell Line, ATCC CCL-228), HepG2 (Human Liver Cancer Cell Line, ATCC HB-8065), HeLa (Human Cervical Cancer Cell Line, ATCC CCL-2), SNU-638 (Human Gastric Cancer) Cellulose, Korea Cell Line Bank KCLB 00638), SNU-668 (Human Gastric Cancer Cell Line, Korea Cell Line Bank KCLB 00668), and AGS (Human Gastric Cancer Cell Line, Korea Cell Line Bank KCLB 21739) After treatment with five herbal extracts, the effect on cell survival was measured.

(1) Cell culture

In this example, TE2, SW480, HepG2 and HeLa cells were cultured in a 37 cm, 5% CO ₂ incubator in a 10 cm diameter cell culture dish containing DMEM medium supplemented with 10% FBS. SNU-638, SNU-668 and AGS cell lines were cultured in a 37 ° C., 5% CO ₂ incubator in a 10 cm diameter cell culture dish containing RPMI medium supplemented with 10% FBS.

(2) Treatment of admiral puffer fish and five herbal extracts of the present invention

On day 1 of the experiment, each cell line culture incubated above was added to be 5 × 10 ² cells per well in a 96-well plate, and 200 μl of medium was added per well. Experiments were conducted in multiples of 6 for each treatment. Blowfish and five kinds of herbal extracts used in this embodiment are extracted in the same manner as in Example 1.

On the second day of the experiment, 1 μl, 2 μl, 4 μl, 10 μl, and 20 μl of the detoxified blowfish and five herbal medicine extracts of the present invention, which were sterilized by filtration with 0.2 μm filters in each well of the 96 well plate, were sterilized. Added. No control wells were treated.

Cell cultures treated with detoxified blowfish and five herbal extracts were incubated for 72 hours in an incubator at 37 ° C., 5% CO ₂ . After 72 hours of treatment with detoxified puffer fish and five herbal extracts, 50 μl of MTT reagent (widely used for cytotoxicity confirmation) was added to each well and incubated in a 37 ° C., 5% CO ₂ incubator for 4 hours. Next, after removing the medium and MTT from each well, 100 μl of DMSO reagent was added to each well. MTT at the bottom of the well was dissolved in DMSO for about 5 minutes, and fluorescence was measured at 570 nm using an ELISA reader.

(3) Effect of Admiralized Blowfish and Five Herbal Medicine Extracts of the Present Invention on Cell Viability

The measurement results are shown in FIGS. 1 to 7. 1 to 7 show the results for SW480, HepG2, TE2, SNU-668, AGS, HeLa and SNU-638 cell lines, respectively. As shown in Figures 1 to 7, when treated with 20 μl of the extract of the present invention, the cell survival rate of the control group when converted to 1, SW480, HepG2, TE2, SNU-668, AGS, HeLa and SNU-638 cell line Were 0.572, 0.586, 0.487, 0.121, 0.063, 0.126 and 0.443, respectively, which showed significant cell killing effect compared to the control. Among the cancer cell lines, AGS cell line was found to be the most sensitive to the cell death effect.

According to the composition for preventing or treating cancer of the present invention, it can be effectively used for preventing or treating cancer.

Figures 1 to 7 are treated with cancer extracts of the mixture of admiral puffer fish and five herbal medicines of the present invention cancer cell lines SW480, HepG2, TE2, SNU-668, AGS, HeLa and SNU-638 cell lines, respectively, the cell survival rate It is a figure which shows each result about.

Claims (4)

Puffer fish detoxified with 1 liter of water; Root skin; Celestial arches; Wolfberry; Earth; And 500-1,500 g of phosphorus mugwort, respectively; 5 to 100 g; 5 to 100 g; 5 to 100 g; 5 to 100 g; And a water extract obtained by distilling the mixture comprising a ratio of 5 to 100 g, and a pharmaceutically acceptable additive. The composition of claim 1, wherein the distillation is performed by distillation in a batch distillation column at a temperature of 100 ° C. to 150 ° C. and a pressure of 1 to 5 kgF / cm ² for 2 to 7 hours. The composition of claim 1 or 2, wherein the cancer is esophageal cancer, stomach cancer, colon cancer, cervical cancer, or liver cancer. The composition of claim 1 or 2, having a formulation selected from the group consisting of liquids, suspensions, emulsions, and syrups.