KR100520896B1 - Method of examining blood type and apparatus for examining blood type using the method - Google Patents

Abstract

Disclosed is a blood type test method and a test apparatus capable of simply testing a blood type required by a single blood injection and storing test results. The blood type test method according to the present invention uses a microchannel to dispose a reagent in each of a plurality of reagent storage chambers, inflowing blood into each of the reagent storage chambers and mixing them with reagents, and a mixture or reactant of blood with a micro filter. Filtering and passing the mixture or reactants through the micro filter in the blood mixture or reactants through a read channel connected to each micro filter. The injected blood is passed through the microchannels to react with the reagents and the result is filtered to ensure that the blood type is clearly and easily identified, even with a small amount of blood, and facilitates reading the results, storing the results and carrying the device. have.

Description

Blood type test method and test device {METHOD OF EXAMINING BLOOD TYPE AND APPARATUS FOR EXAMINING BLOOD TYPE USING THE METHOD}

The present invention relates to a blood type test method and a test apparatus, and more particularly, to a device that can simply test the blood type using a single channel using a micro-channel and a micro filter.

In 1900, Karl Landsteiner argued that the incidence of severe transfusion reactions in the transfusion of human blood to others is due to the hemolysis of red blood cells by isoagglutinin, which is responsible for MNS, P, Rh, 23 blood groups, including Lutheran, Kell, and Lewis, are classified. The classification of blood types is a basic task to avoid hemolytic transfusion side effects in case of transfusion such as surgery, war, etc. The presence or absence of antibodies to erythrocyte antigens is an important factor that determines the success of blood transfusion.

For this reason, ABO blood test, Rh blood test, antibody screen test, etc., which are essential blood test items, are performed in a conventional hospital or a general physical examination. However, conventionally, the test method used in hospitals mainly prepares an antibody to be sampled on a glass slide and drops blood to the antibody to test agglutination reactions one by one. I'm using it.

The method of using glass slides requires manual inspection by the inspector, and blood test results must be obtained every time since the blood test results cannot be stored for a long time after the test is performed. In addition, after describing the test results, there is a problem in that it is troublesome and unsanitary to wash for reuse of the slide. In addition, when a large number of samples have to be processed, there is a possibility of misprinting when the test results are transferred, and there is a risk that the inspector is always exposed to the patient's blood because the cleaning must be performed every time. In addition, there is a problem that the inspection results thereafter may be different due to incomplete cleaning. In addition, the method using the glass slide also has a drawback that the objectivity of the inspection result can be significantly lowered because it depends on the judgment of the inspector.

In addition, there are expensive test devices provided in the form of chips, but these devices require separate expensive equipment such as chip incubators, dedicated centrifuges, samplers, and readers to be used for blood test chips. The use of the equipment provides the inspector with economic burdens as well as local problems.

As described above, the conventional blood test method and apparatus have problems such as burden of purchase, difficulty of use, difficulty of storage, objectivity of the test, and the like. In particular, there is a difficulty in obtaining a certain amount of blood volume even when testing blood of a newborn or a child who is difficult to take a blood sample.

Accordingly, it is an object of the present invention to provide a simple and inexpensive blood type test method and test apparatus capable of obtaining sufficient results even with a small amount of blood samples.

Another object of the present invention is to provide a blood type test method and test apparatus capable of ensuring objectivity of blood type tests and storing test results.

Still another object of the present invention is to provide a blood type test apparatus having low manufacturing cost and easy mass production.

According to a preferred embodiment of the present invention for achieving the above object of the present invention, the blood type test method relates to a method for testing the blood type by reacting the same blood sample with a predetermined reagent, the method according to the invention Forming a reagent storage chamber to place one or more types of reagents in each chamber, introducing a blood sample into each of the reagent storage chambers and mixing with the reagents, a mixture or reactant of the blood sample and the reagents with a micro filter Filtering the mixture and passing the mixture or reactants of the mixture or reactants that have passed through the microfilters to a read channel connected to each of the microfilters.

The present invention utilizes the property that the injected blood sample forms a different mixed state or a different reaction state in response to a specific reagent, and filters the mixture or reactants having the different states according to the specific reagent passing through the filter. How to read blood type.

According to a general example, when determining the ABO blood type, antigens corresponding to the A and B types are placed in the reagent storage chamber, and then blood is introduced into the respective reagent storage chambers to generate an antigen-antibody reaction to form different states. can do. The injected type A blood sample causes an antigen-antibody reaction with antigen A and no antigen-antibody reaction with antigen B. As a result, the blood mixed with the antigen A causing the aggregation reaction cannot pass through the micro filter, and the blood in the other storage chamber mixed with the antigen B can be adjusted to pass through the micro filter. Able to know. This method can be applied corresponding to blood samples of type B, AB and O.

In addition to the test of the ABO blood group, it is also possible to read a blood type defined differently, such as the Rh blood group by adjusting the reagent corresponding to the blood.

According to a preferred embodiment, the method according to the invention further comprises positioning a blood sample in a blood injection chamber and introducing a blood sample located in the blood injection chamber into a plurality of microchannels. The blood sample introduced into the blood injection chamber is introduced into the microchannel connected to the blood injection chamber by capillary action and into the reagent storage chamber with each reagent. By forming one blood injection chamber and forming a microchannel connecting the blood injection chamber and each reagent storage chamber, a single dose can simultaneously feed the same amount of blood into a plurality of reagent storage chambers at the same time and a small amount of blood. Samples can be properly dispensed to minimize the amount of blood sample required.

According to a further preferred embodiment, the method according to the invention can facilitate the reading of blood passing through the reading channel by forming a reading chamber in the reading channel. It is possible to form a circular read chamber having a diameter wider than the width of the read channel on the read channel. By forming a reading window on the upper surface of the reading chamber, reading from the outside can be made easier.

It is preferred that a first blood resistance is formed between the reagent storage chamber and the micro filter to prevent the reagent disposed in the reagent storage chamber from flowing out into the micro filter before mixing with the blood. Specifically, by forming a first resistance channel between the reagent storage chamber and the micro filter and hydrophobic treatment on a portion of the inner wall of the first resistance channel, it is possible to prevent the reagent from being discharged into the filter without remaining in the reagent storage chamber. Hydrophobic treatment means that a portion of the wall of the microchannel is hydrophobic so that an aqueous solution or other fluid does not readily pass through the hydrophobic region. It can be made hydrophobic mainly by chemical treatment on the wall of the channel.

This hydrophobic treatment may also be applied to form a second blood resistance at the end of the read channel. In order to determine whether the blood mixture or blood reactant mixed with the reagent has passed through the filter, it is desirable to stagnate the blood mixture or blood reactant on the reading channel, in particular when forming a low pressure at the end of the reading channel and inhaling the blood. Resistance can be formed to prevent blood mixture or reactants from being discharged by suction.

According to another preferred embodiment of the present invention, the blood type test apparatus of the present invention is a blood injection chamber, a plurality of microchannels having one end connected to the blood injection chamber, a plurality of reagent storage chambers respectively connected to the other end of the microchannel, reagent storage And a plurality of micro filters each connected to the chamber and a plurality of read channels connected to each micro filter.

The microchannel may be a straight or curved microchannel connecting the blood injection chamber and each reagent storage chamber one-to-one, and may be a circuit composed of microchannels branched from one microinfusion channel to a plurality of reagent storage chambers. .

A micro filter for filtering a mixture of blood samples and reagents or reactants is connected to the reagent storage chamber.

The micro filter includes a filter chamber and a plurality of filter columns formed in the filter chamber. The filter columns may be arranged in parallel in narrow intervals in the flow direction of the fluid passing through the filter to form a plurality of holes, and only particles having a size that can pass between the filter columns will pass through the filter. The filter structure is composed of a filter chamber and a plurality of filter columns, and various filter column structures may be used to pass only contents of a predetermined size.

A first blood resistance may be formed between the reagent storage chamber and the micro filter, and a second blood resistance formed adjacent the read channel end is preferably formed. The blood resistance temporarily interrupts the flow of fluid through the channel to prevent leakage of the fluid, provides time for the fluid to mix sufficiently, and temporarily stops the fluid to facilitate reading of the fluid through the filter. Function to save.

According to a preferred embodiment, the hydrophobic treatment is applied to a portion of the wall of the channel through which the fluid flows, so that the fluid passing through the channel of the hydrophobic treated portion is disturbed in flow. The first blood resistance portion is disposed between the reagent storage chamber and the micro filter and includes a first resistance channel and a first hydrophobic surface treatment to prevent reagent stored in the reagent storage chamber from entering the micro filter before being mixed with blood, It can interrupt the flow of the mixture of blood and blood to provide time for sufficient reaction.

The second blood resistance portion is disposed at the end of the reading channel, and when the reading chamber is formed, it is disposed opposite the first blood resistance portion with the reading chamber therebetween. The second blood resistance portion includes a second hydrophobic surface treatment portion formed on at least one inner surface of the read channel and has hydrophobicity, and facilitates reading by temporarily blocking an end of the read channel.

An inlet is formed at the end of the reading channel. A suction port may be formed at each channel end, and preferably, one suction port is connected with a plurality of read channels to provide suction force to easily pass through the channel of a mixture of blood, blood and reagents.

Hereinafter, the present invention will be described in detail with reference to the accompanying drawings, but the present invention is not limited or restricted by the embodiments.

Example 1

1 is a schematic diagram illustrating a blood type test method and a test apparatus according to a first embodiment of the present invention.

Referring to FIG. 1, the blood type test apparatus according to the first exemplary embodiment includes two reagent storage chambers 130 and 135, micro filters 140 and 145 connected to each reagent storage chamber 130 and 135, and other ends of the micro filters 140 and 145. Read channels 150 and 155.

Samples of blood (BLD) drawn from a particular person are injected into reagent storage chambers 130 and 135 directly or via injection means such as syringes. In the reagent storage chambers 130 and 135, reagents capable of reading blood, that is, antigens A and B, are stored in different reagent storage chambers, respectively. Antigen-antibody reactions can occur when blood (BLD) mixes with either A or B antigens. When the aggregation reaction occurs, the aggregated blood mixture cannot pass through the filter, and when the aggregation reaction does not occur, the blood mixture passes through the filter and becomes readable through the read channel.

First blood resistance portions 160 and 165 are formed between the reagent storage chambers 130 and 135 and the microfilters 140 and 145. The first blood resistance parts 160 and 165 include a first resistance channel and a first hydrophobic surface treatment part formed on a bottom surface of the first resistance channel. The first hydrophobic surface treatment unit prevents the reagents stored in the reagent storage chambers 130 and 135 from entering the microfilters 140 and 145 before mixing with the blood, and allows sufficient time for sufficient mixing after the blood and the reagents are mixed. The blood mixture may be confined to provide.

After sufficient time has elapsed, the test device is tilted or the readout channels 150, 155 to allow the mixture or reactants in each reagent storage chamber 130,135 to pass through the first blood resistance 160,165 and into the microfilters 140,145. A method of sucking the fluid by connecting the suction device to the end of the) may be applied.

In the present embodiment, the antigens A and B are used as reagents to determine the ABO blood group, but the present invention is not limited thereto, and other ABO blood test methods or other types of blood test methods and other antigen-antibody reactions may be mixed. If it can be distinguished using a result filter, it can be examined by the present invention.

Example 2

Figure 2 is a schematic diagram for explaining the blood type test method and test apparatus according to a second embodiment of the present invention.

Referring to FIG. 2, in the blood type test apparatus according to the second embodiment, one end is connected to the lower side of the blood injection chamber 220 and the blood injection chamber 220, and the other end is divided into two fine channels 225, which are branched. Two reagent storage chambers 230 and 235 connected to the ends of the microchannels 225, two micro filters 240 and 245 connected to the respective reagent storage chambers 230 and 235, and read channels 250 and 255 connected to the other ends of the micro filters 240 and 245, respectively. ).

According to the present embodiment, the blood injection chamber 220 and the reagent storage chambers 230 and 235 form a cylindrical chamber, and the microchannels 225 or the micro filters 240 and 245 are connected to the bottom of each of the chambers 220, 230 and 235.

As in the first embodiment, a blood (BLD) sample drawn from a particular person is injected into the blood injection chamber 220 either directly or via injection means such as a syringe. The reagent storage chambers 130 and 135 store reagents capable of reading blood, that is, antigens A and B, respectively, in different reagent storage chambers. Antigen-antibody reactions can occur when blood (BLD) is automatically dispensed at the same time and mixed with antigen A or antigen B. When the aggregation reaction occurs, the aggregated blood mixture cannot pass through the filter, and when the aggregation reaction does not occur, the blood mixture passes through the filter and becomes readable through the read channel. Of course, by adjusting the filter size of the micro filter (240 245), it is possible to control the passage of the blood mixture in which the aggregation reaction occurs.

For example, when antigen A is located in the left reagent storage chamber 230 and antigen B is located in the right reagent storage chamber 235, the right reagent storage chamber 235 is provided if blood of type B is injected into the blood injection chamber 220. Aggregation reaction with the antigen B occurs in the) and does not occur in the left reagent storage chamber (230). Therefore, the blood mixed with the antigen A may pass through the left micro filter 240 and may be read through the read channel 250, and the blood mixed with the antigen B may cause the aggregation reaction to pass through the right micro filter 245. Cannot be read through read channel 255.

First blood resistances 260 and 265 are formed between the reagent storage chambers 230 and 235 and the micro filters 240 and 245. The first blood resistance parts 260 and 265 include a first resistance channel and a first hydrophobic surface treatment part formed on a bottom surface of the first resistance channel. The first hydrophobic surface treatment unit may prevent the reagents stored in the reagent storage chambers 230 and 235 from entering the microfilters 240 and 245 before mixing with the blood, and allow sufficient time for sufficient mixing after the blood and the reagents are mixed. The blood mixture may be confined to provide.

After sufficient time has elapsed, the method or the readout channels 250,255 for tilting the test device to allow the mixture or reactants in each of the reagent storage chambers 230,235 to pass through the first blood resistances 260,265 into the microfilters 240,245. A method of sucking the fluid by connecting the suction device to the end of the) may be applied.

In the present embodiment, the antigens A and B are used as reagents to determine the ABO blood group, but the present invention is not limited thereto, and other ABO blood test methods or other types of blood test methods and other antigen-antibody reactions may be mixed. If a result can be distinguished using a filter, it can be examined by the method or apparatus of the present invention.

Example 3

3 is a perspective view of a blood type test apparatus according to a third embodiment of the present invention, FIG. 4 is a plan view of the blood type test apparatus of FIG. 3, and FIG. 5 is a cross-sectional view illustrating the I-I cross section of FIG. 4.

3 to 5, the blood type test apparatus 300 according to the third embodiment includes a base plate 305, a chip plate 310 and a chip plate 310 disposed on an upper surface of the base plate 305. Blood reagent chamber 320 formed at the left center, four reagent storage chambers 330 formed in a line on the chip plate 310 adjacent to the blood injection chamber 320, the blood injection chamber 320 and the respective reagent storage chambers Four microchannels 325 connecting 330, a first blood resistance part connected to each reagent storage chamber 330, four microfilters 340 connected to each first blood resistance part, and each micro filter ( Four read channels 350 connected to the ends of the 340, four read chambers 380 disposed on each read channel 350 to form a readout window, and one suction port connected to the ends of the read channels 350 390 and second blood resistance portion 382 disposed between the end of read channel 350 and read chamber 380 And a.

In the present embodiment, the base plate 305 is made of transparent glass of a rectangular parallelepiped, and a chip plate 310 made of a polymer material is disposed on the base plate 305. The bottom surface of the chip plate 310 in contact with the base plate 305 may include a blood injection chamber 320, a microchannel 325, a reagent storage chamber 330, a micro filter 340, a read channel 350, and a read chamber ( 380 and inlet 390 are formed, and these components are formed intaglio on the bottom of the chip plate 310 by molding a polymer material.

As the polymer material constituting the chip plate 310, synthetic resins such as polymethyl methacrylate (PMMA), polycarbonate, polytetrafluoroethylene (TEFLON, polyvinyl chloride (PVC), and polydimethylsiloxane (PDMS) are used. do.

The blood injection chamber 320 is formed at the center adjacent to one end of the chip plate 310. One end of the microchannel 325 is connected to the lower end of the blood injection chamber 320 and the other end is connected to the other reagent storage chamber 340, respectively. In the present embodiment, the reagent storage chamber 340 stores anti-A, anti-B, and anti-D, and an amount of about 2 to 5 μl or less is used.

About 10 μl or less of blood (BLD) is injected into the blood injection chamber 320 while the blood flows into the reagent storage chamber 330 through the microchannel 325 by the suction force or capillary action of the channel. . Blood causes different reactions according to blood types while causing a mixture or aggregation reaction with the antibodies stored in the reagent storage chamber 330, respectively.

After sufficient time has elapsed for the aggregation reaction to occur, the test device is tilted or a suction force is applied through the suction port 390 formed in the test device to introduce the blood mixture or the reactant into the micro filter 340.

As in the first and second embodiments, the blood reactant that has undergone the aggregation reaction cannot pass through the micro filter 340, and other blood mixtures can pass through the micro filter 340 to be identified through the read channel 350. have.

FIG. 6 is a partially enlarged plan view illustrating an enlarged portion A to explain the blood type test apparatus of FIG. 4.

Referring to FIG. 6, the micro filter 340 according to the present embodiment has two stages of filter layers. A first filter portion 344 having a large spacing between the filter structures is formed adjacent to the reagent storage chamber 330, and a second filter portion 346 having a narrow spacing between the filter structures adjacent to the reading channel 350 is formed. Include. Referring to the illustrated first and second filter parts 344 and 346, each filter part 344 and 346 is arranged with a plurality of filter columns having an elongated cross-section having a longer length than a length to form a filter structure. A plurality of filter columns are regularly arranged at regular intervals in the first direction, and a plurality of filter columns adjacent thereto are regularly arranged at regular intervals in a second direction that is staggered with the first direction.

In the present embodiment, the filter columns of the first filter portion 344 are disposed at about 100 μm intervals in the first direction at an angle of 45 degrees to the right in the fluid flow direction, and the adjacent filter columns are 45 to the left in the fluid flow direction. It is arranged at intervals of about 100 μm in a second direction forming an angle. The filter columns of the second filter part 346 are arranged in a shape similar to the filter columns of the first filter part 344, but the distance between the filter columns is about 50 μm, and the size of the filter columns is about 1/2 of the filter columns. Is formed to the size of. The filter pillars of the first and second filter portions 344 and 346 are formed through molding when the chip plate 310 is molded, and the bottom surface of the filter pillar contacts the base plate 305 to form a pillar in the filter chamber.

The shape of the filter column and the arrangement of the filter column may be designed in various shapes and arrangements according to the designer's choice. 8 and 9 are partially enlarged plan views showing another embodiment of the micro filter. The present invention is not limited to the shape and arrangement of the filter pillars shown in FIGS. 6, 8 and 9.

A first blood resistance portion is formed between the reagent storage chamber 330 and the micro filter 340 to store a reagent stored in the reagent storage chamber 330 or to confine the contents in the chamber while reacting with the blood. In the present exemplary embodiment, the first blood resistance part includes a first hydrophobic surface treatment part 342 disposed on the first resistance channel and the base plate 305 in a transverse direction of the channel to form a hydrophobic surface on the bottom of the first resistance channel. Include. Hydrophobicity is formed at the bottom of the first resistance channel to prevent the reagent from flowing out before mixing with the blood or entering the filter before the blood mixture sufficiently reacts.

7 is an exploded perspective view of the blood type test apparatus of FIG.

Referring to FIG. 7, before placing the chip plate 310 in which the blood injection chamber 320, the microchannel 325, the reagent storage chamber 330, the micro filter 340, etc. are formed on the base plate 305. First and second hydrophobic surface treatments 342 and 382 are formed at predetermined positions on the base plate 305.

Various methods can be provided as a method for forming the hydrophobic surface. In this embodiment, octadecyltrichlorosilane: CH ₃ (CH ₂ ) ₁₇ SiCl ₃ (hereinafter referred to as “OTS”) is used for hydrophobic treatment on the base plate 305 made of glass. After applying a solution of OTS and hexane 1: 200 on the base plate 305, the hexane is washed with a solution of 1: 1 mixed with hexane and methanol (methanol). After washing hexane, methanol is dried with nitrogen (N ₂ ) to form an OTS layer on the base plate 305. After forming a photomask on the OTS layer and irradiating ultraviolet rays with a wavelength of about 400 nm, the exposed portion is hydrophilic and the hidden portion is hydrophobic.

In the present embodiment, as described above, the first and second hydrophobic surface treatment units 342 and 382 are formed using the OTS, but the present invention is not limited thereto, and one of various methods for forming a hydrophobic surface on the base plate may be selected and used. Can be.

After sufficient time (about 3 minutes or less) for the blood and reagents to mix, the test device is tilted or a suction force is applied to the suction port 390 to allow the mixture or reactant of the blood and reagents to pass through the first blood resistance and the microfilter. Inflow to (340). The suction port 390 is connected to the end of the reading channel 350, and may form a suction force by connecting a syringe or a separate suction device to one suction port 390.

The blood mixture that has passed through the micro filter 340 may be read through the read channel 350. A reading chamber 380 is formed in the reading channel 350 to facilitate reading of the blood mixture, and the wide top surface of the reading chamber 380 is made transparent to form a reading window for easier reading.

A second blood resistance is formed between the reading chamber 380 and the end of the reading channel 350 to facilitate reading. The second blood resistance portion includes a second hydrophobic surface treatment portion 382 formed on the channel bottom of the read channel 350. Since the second hydrophobic surface treatment unit 382 in the present embodiment is the same as the method of forming the first hydrophobic surface treatment unit 342 above, a repeated description relating to the second hydrophobic surface treatment unit 382 is omitted.

The second blood resistance part confines the blood mixture in the reading chamber 380 to prevent it from flowing out through the end of the reading channel 350, and flows through the reading channel 350 into another neighboring reading channel 350 and flows back. Function to prevent it.

According to the present invention, since a small amount of blood is injected only once, the blood type in the required manner can be easily tested, and thus a quick blood test is possible. Such a convenient feature of the present invention requires an emergency room, an accident scene, etc. It can be usefully used.

In addition, since only a small amount of blood to test the blood type is necessary, it is suitable for judging blood types of children and newborns.

In addition, it is easy to read and store the blood test results, it is easy to store the results, there is little possibility of a substrate error, it can be the basis for realizing the test automation.

In addition, the risk of infection to the outside is small and can be performed immediately at the same time as blood collection.

As described above, although described with reference to a preferred embodiment of the present invention, those skilled in the art will be variously modified and modified within the scope of the present invention without departing from the spirit and scope of the present invention described in the claims below. It will be appreciated that it can be changed.

1 is a schematic diagram illustrating a blood type test method and a test apparatus according to a first embodiment of the present invention.

Figure 2 is a schematic diagram for explaining the blood type test method and test apparatus according to a second embodiment of the present invention.

3 is a perspective view of a blood type test apparatus according to a third embodiment of the present invention.

4 is a plan view of the blood type test apparatus of FIG.

FIG. 5 is a cross-sectional view illustrating the I-I cross section of FIG. 4.

FIG. 6 is a partially enlarged plan view illustrating an enlarged portion A to explain the blood type test apparatus of FIG. 4.

7 is an exploded perspective view of the blood type test apparatus of FIG.

8 and 9 are partially enlarged plan views showing another embodiment of the micro filter.

<Explanation of symbols for the main parts of the drawings>

300: blood type test device 305: base plate

310: chip plate 320: blood injection chamber

330 reagent storage chamber 340 micro filter

350: reading channel 380: reading chamber

390: suction port

Claims (29)

In the blood type test method for testing blood types by reacting blood with a reagent, Placing a reagent in each of the plurality of reagent storage chambers; Introducing blood into each of the reagent storage chambers and mixing the reagents with the reagents; Filtering the blood or mixture of reactants or reactants with a micro filter; And Passing the mixture or reactants of the mixture or reactants that have passed through the microfilters into a read channel connected to each of the microfilters, The micro-filter includes a filter chamber and a plurality of filter pillars formed in the filter chamber, the blood type test method, characterized in that it comprises a filter to block the flow of the blood mixture or reactants flowing through the filter. The method of claim 1, The test method comprises the steps of injecting blood into the blood injection chamber; And dividing and injecting blood injected into the blood injection chamber into a plurality of microchannels, wherein the microchannels are connected to the respective reagent storage chambers so that the blood passing through the microchannels passes through the microchannels. Blood type test method, characterized in that flowed into. The method of claim 1, And a reading chamber is formed in said reading channel. The method of claim 1, And a first blood resistance portion is formed between the storage chamber and the micro filter. The method of claim 4, wherein And a first hydrophobic surface treatment unit having a hydrophobicity on at least one inner surface of the first resistance channel connecting the storage chamber and the micro filter to the first blood resistance unit. . The method of claim 4, wherein And a second blood resistance portion is formed adjacent the end of the read channel. The method of claim 6, And the second blood resistance part comprises a second hydrophobic surface treatment part having hydrophobicity on at least one inner surface of the read channel. delete The method of claim 1, And said filter portion comprises at least two filter portions arranged in series, wherein said filter column spacing closer to said reagent storage chamber is wider than other filter column spacing of said other filter portion further distant. The method of claim 9, The microfilter includes a first filter part and a second filter part. As a blood type test device, Blood injection chamber; A plurality of microchannels having one end connected to the blood injection chamber; A plurality of reagent storage chambers each connected to the other end of the microchannel; A plurality of micro filters each connected to the reagent storage chamber; And A plurality of read channels each connected to the micro filter, Each micro filter comprises a filter chamber; And a filter unit forming a plurality of filter pillars formed in the filter chamber to prevent a blood mixture or a reactant flowing through the filter. The method of claim 11, The microchannel is a blood type test device, characterized in that for connecting the blood injection chamber and each of the reagent storage chamber in a one-to-one. delete The method of claim 11, Cross section of the filter column is longer than the longitudinal length, blood type test apparatus, characterized in that arranged in the direction of the flow of the fluid passing through the micro filter. The method of claim 11, And a first blood resistance portion formed between the reagent storage chamber and the micro filter. The method of claim 15, And a first hydrophobic surface treatment unit having a hydrophobicity on at least one inner surface of the first resistance channel connecting the storage chamber and the micro filter to the first blood resistance unit. . The method of claim 15, And a second blood resistance portion formed adjacent the end of said reading channel. The method of claim 17, And the second blood resistance part is formed on at least one inner surface of the read channel to form a second hydrophobic surface treatment part having hydrophobicity. The method of claim 11, And a suction port is formed at an end of the reading channel. The method according to any one of claims 11, 12, or 14 to 19, And a reagent is stored in the reagent storage chamber. delete As a blood type test device, Base plate; A chip plate disposed on the base plate; A blood injection chamber formed in the chip plate; The plurality of microchannels formed on the chip plate and having one end connected to the blood injection chamber; A plurality of reagent storage chambers formed on the chip plate and connected to the other ends of the microchannels, respectively; A plurality of micro filters formed on the chip plate and connected to the reagent storage chambers, respectively; A plurality of read channels formed on the chip plate and connected to the micro filters, respectively; And A readout chamber positioned in the readout channel and forming a transparent or translucent readout window on an upper surface thereof, Each micro filter comprises a filter chamber; And And a filter unit which forms a plurality of filter pillars formed in the filter chamber and prevents the flow of the blood mixture or the reactant flowing through the micro filter. The method of claim 22, The chip plate is composed of one synthetic resin selected from the group consisting of polymethyl methacrylate (PMMA), polycarbonate, polytetrafluoroethylene (TEFLON, polyvinyl chloride (PVC) and polydimethylsiloxane (PDMS), And the blood injection chamber, the microchannel, the reagent storage chamber, the microfilter, and the readout channel are engraved on the bottom surface of the chip plate in contact with the base plate. delete The method of claim 22, The filter column has a horizontal length longer than the vertical length, blood type test device, characterized in that the regular arrangement of the T-shaped vertical relationship at regular intervals with the other filter columns around. The method of claim 22, And a first blood resistance part including a first resistance channel connecting the storage chamber and the micro filter and a first hydrophobic surface treatment part disposed on a bottom surface of the first resistance channel and having a hydrophobicity. . The method of claim 22, And a second blood resistance portion disposed between an end of the reading channel and the reading chamber and including a second hydrophobic surface treatment portion hydrophobically treating the bottom surface of the reading channel. The method of claim 22, And a suction port connecting the ends of the read channel to one. The method according to any one of claims 22, 23, or 25 to 28, And a reagent is stored in the reagent storage chamber.