KR100504767B1 - A functional food Containing herbes composition for allergy disease treatment - Google Patents

Abstract

The present invention relates to a functional food having an allergic disease symptom improving activity, and more particularly, Euiin, baekbaekpi, Eoseongcho, Giltyeong, raw yellow sulfur, gold and silver, triticale, ginger, dermis, baekchul, creation, Astragalus, licorice, mint The present invention relates to a functional food having an active function of improving allergic disease symptoms containing as a main ingredient herbal medicines such as red pepper, leek, jujube and jujube.

Functional food having an active function to improve the symptoms of allergic diseases according to the present invention can be used regardless of the cause and constitution, and even without prolonged use, it has the characteristics that there are no side effects such as digestive disorder, dizziness and heartburn.

Description

A functional food containing herb composition for improving allergic disease symptoms

The present invention relates to a food additive having an allergic disease symptom improving function and a functional food containing the food additive, and more particularly, Euiin, baekbaekpi, eoseongcho, Gilgyeong, raw yellow sulfur, gold silver, triticale, ginger, dermis, baekchul It is a food additive that has the function of improving allergic disease symptoms regardless of constitution and symptom according to the Chinese medicine diagnostic method as a main ingredient, creation, Astragalus, licorice, peppermint, red pepper, green onion, jujube and herbal medicine And it relates to a functional food containing the food additive.

In general, the treatment of allergic diseases is mainly used for the treatment of drugs such as antihistamines, antibiotics or hormones, and when taking the above-mentioned treatments, side effects such as digestive disorders, dizziness and heartburn appear due to its toxicity. In addition, there has been a problem that allergic symptoms can become chronic by having resistance to allergens when taken for a long time.

Recently, the development of allergic diseases using herbal ingredients has been attempted.

Korean Patent Registration No. 354608 is a prescription of Hyeonggae Yeongyo-tang (荊芥 連 翹 湯) as a base room and a treatment agent to treat otitis media and wind fever, and allergies such as acute allergic rhinitis, asthma, dermatitis and otitis media. Although a composition for treating a disease is disclosed, the above prescription is necessarily taken after a meal, and does not consider gastrointestinal conditions. Depending on the type of dialectic, if the fever is not a cause, the effect may be insignificant or may be different. .

In addition, the recent research on allergic diseases using herbal medicines shows that Kamitonggyutang, Hwangyeontongseongsan, Kamibangpungtongseongsan, and Kwangwansan (通 關 散), Hyunggaeyeon Gyotangami (荊芥 連 翹 湯加 味), etc. The administration of this disease has been reported significantly, but the effect was insignificant or discomfort to take constitution and symptom.

On the other hand, in oriental medicine, allergic diseases include cold, wind, moist fever, external heat such as diarrhea, ruins, deprivation, and kidney pain. It is said to be caused by internal or external causes such as internal or external labor such as daylight or daylight. In clinical practice, allergic diseases are most often caused by undefined weakness. Many literatures have been reported on the association between allergic diseases and the digestive system. In particular, it is known that allergic diseases are closely related to not only contaminated air but also food problems, that is, gastric fluoride caused by excessive intake of fast foods, fast foods, and carbonated drinks.

In addition, in Korean medicine, the lungs control the qi and the nasal cavities are the origin of the flow of the qi, and both the lungs and the nasal organs are qi. ) And Sujin are closely related to each other, and their pathology is also affected. In other words, if weakness is weak, it leads to a lack of disposal, which leads to aberrant action of the lungs and stagnation of fluid. Condensation occurs for a long time, causing allergic rhinitis. In addition, the lungs and the intestines are related to the yin and yang of the upper and lower limbs, "the lungs are paired with the large intestine, and the intestines correspond to the skin (youngchu, this chapter)" is closely related to the problems of the digestive system.

Allergic diseases are caused by excessive allergens in the body's immune system, causing tumor necrosis factor-α (TNF-α), interleukin-1 (IL-1), and interleukin-6 (IL-6) in immune cells such as macrophages. 6) excessively induces inflammatory substances, such as prostaglandin, luecotriene and nitric oxide (NO), leading to inflammatory reactions resulting in allergic rhinitis, atopic dermatitis, chronic asthma And immune hypersensitivity symptoms are known.

Therefore, the present invention is to provide a development of a functional food that can improve the symptoms of allergic diseases regardless of the cause and constitution of herbal medicines by combining herbal medicines and to develop functional foods added to various foods.

The present inventors are the right to strengthen the intestine (폐) and lung (각각), Sangbaekpi, Eoseongcho, Gilgyeong; Raw turmeric, gold and silver, three hundred seconds, ginger, dermis, whiteness, creation, astragalus, licorice, peppermint, antagonism to help stomach and stomach And food additives containing herbal medicines of leek, jujube and soybeans as main ingredients on lung, nasal, renal, stomach, large intestine, and large intestine, regardless of the cause and constitution In animal experiments, it was confirmed that there is an effect of inhibiting NO production, PGE2 production, TNF production, and transcription factor NF-κB activation in cell lines stimulated with.

An object of the present invention is to provide a functional food to have an allergic disease symptom.

It is another object of the present invention to provide a functional food that has an improvement in allergic disease symptoms regardless of the cause and constitution.

In addition, the present invention uses a herbal composition containing Euiin, baekbaekpi, Eoseongcho, Gilgyeong, raw sulfur, gold and silver, three hundred seconds, ginger, dermis, baekchul, creation, Astragalus, licorice, mint, red pepper, green onion, jujube and medicinal herbs as main components This is an allergic disease symptom that does not show side effects such as digestive disorders, dizziness and heartburn, even if it continues to be used for a long time by acting on the lungs, rain, kidneys, kidneys, stomach, and large intestine. An object of the present invention is to provide a functional food that has an improvement function.

In order to achieve the above object, the present invention is the main ingredients of Euiin, Sangbaekpi, Eoseongcho, Gilgyeong, Saenghwanghwang, Geumgeunhwa, three hundred seconds, ginger, dermis, Baekchul, creation, Astragalus, licorice, peppermint, red pepper, green onion, jujube and the main medicine Provides a functional food to have the function of improving the symptoms of allergic diseases containing.

In another aspect, the present invention provides a functional food to have an improvement in allergic disease symptoms that can be used regardless of the cause and constitution.

In addition, the present invention is an allergic disease that does not show side effects such as digestive disorders, dizziness, and heartburn, even if it continues to be used for a long time because it acts on the lungs, rain, kidneys, kidneys, stomach, and large intestine. Provide functional foods to improve symptoms.

Referring to the present invention in more detail as follows.

The present invention is an allergic disease symptom that contains Euiin, baekryepi, eoseongcho, Gilyeong, raw sulfur, gold and silver, three hundred seconds, ginger, dermis, baekchul, creation, Astragalus, licorice, peppermint, red pepper, green onion, jujube and seed Provide functional foods with improved function.

Herbal medicine Uiin used in the present invention is used to clean the lungs, to help the nasal secretion, to pulmonary, janggong (腸 癰), drainage, small seed (消腫) has the efficacy, and is used to treat several species (水腫 脚氣). Epithelium is used for bronchitis by lowering the heat of the lungs, stopping coughs, and relieving breathing symptoms, and helps lower blood pressure. Eoseongcho has excellent detoxification, anti-inflammatory action, and the function of the intestines to activate and clear blood. Gilyeong acts on menopause to clear the walls, stop coughing, and allow for good disposal. Saengjihwang detoxification, intestinal melts, stops bleeding and lowers fever. Gold and silver are clear and detoxifying, inflammation and to remove the boil pus. Three hundred seconds is very good detoxification and diuretic, eliminates inflammation, has anticancer activity, eliminates constipation and stool, and is used to treat and prevent hypertension and atherosclerosis. Ginger promotes metabolic functions, acts as an antitussive, protects the stomach, aids in digestion, works well for motion sickness, and suppresses the rise of blood cholesterol. The dermis acts on the spleen and lungs, helping the circulation of the group to fight edible, vomiting, and coughing. Baekchul relieves the stomach and breaks down the food while eating. Creation eliminates moisture, heals the secretions and evens the stomach and eats well. Astragalus is effective in tonic, cold, diuretic and small-species, so it has physical weakness, fatigue boredom, bleeding deprivation, disinfection, uterine drainage, visceral sewage, and cold Good effect on sweat. Licorice helps to nourish, refresh, and harmonize rot, cleansing, detoxification and pharmaceuticals. Peppermint has a dissipative wind fever and Cheongryong throat. Jeokjak enhances the action of gear blood agents, such as active blood fish, blood mania, pulmonary pulmonary disease, and swelling poisoning. .

The present invention is yiyipi, Sangbaekpi, Eoseongcho, Gilgyeong, respectively, to strengthen the intestines and lungs; Raw turmeric, gold and silver, three hundred seconds, ginger, dermis, whiteness, creation, astragalus, licorice, peppermint, antagonism to help stomach and stomach And functional foods to have the function of improving the symptoms of allergic diseases containing the herbal medicines of green onions, jujube and chaff as a main component.

The food additive containing the herbal composition of the present invention is allergic by inhibiting the production of NO, the production of PGE2, the production of TNF and the activation of the transcription factor NF-κB in LPS-stimulated cell lines, as can be seen in the following examples. Allergic rhinitis associated with the disease, atopic dermatitis, chronic asthma and immune hypersensitivity symptoms can be improved, and can be used regardless of the cause and constitution of the Chinese medicine, and the present invention, lung (肺), nasal (脾) , Kidney (,), stomach (대), large intestine (大腸) acts long-term use does not show side effects such as digestive disorders, dizziness and heartburn.

The present invention is the herbal medicine, Euibaekpi, Eumseongcho, Gilseong, Saenggihwang, gold and silver, three hundred seconds, ginger, dermis, baekchul, creation, Astragalus, licorice, peppermint, red pepper, green onion, jujube and medicinal herbs are mixed to add water The extract is prepared by extraction or by adding an aqueous alcohol solution, followed by extraction under reduced pressure, spraying, or lyophilization. Hot water extraction according to the present invention can be extracted twice after 4 hours at 80 ℃ after adding purified water, and when the aqueous solution of alcohol is added, the aqueous alcohol solution can be selected from 5 to 100% ethanol or methanol, and more Preferably 70% ethanol is used. In addition, the present invention can be prepared by concentrating the extract in a vacuum concentrator, and then spraying or freeze-drying to prepare a powder of the extract.

Although it is preferable that the compounding ratio of each component in the herbal composition which concerns on this invention is set variously according to their kind, For example, the following compounding ratios are preferable.

Herbal medicine composition according to the present invention is about 1: 4 ~ 1 ~ 0.7 ~ 1, raw kohwang, gold and silver, three hundred seconds, ginger, dermis, baekchul, creation, Astragalus, licorice, peppermint and red tide 1: 0.1 ~ 1, leek is 1: 1-10, jujube is 1: 0.1 ~ 1, soybean is 1: 0.01 ~ 0.1, more preferably for herbal medicine yiyiin is 1: 1, Eoseongcho, Gil-Gyeong are 1: 0.75, raw sulfur, gold and silver, three hundred seconds, ginger, dermis, white, creation, Astragalus, licorice 1: 0.5, mint 1: 0.4, red 1: 0.2, leek 1: 5, jujube 1: 0,4 And molar is 1: 0.01.

In addition, according to the present invention, the present ginseng, health, half, yeonkyo, jisil, longan meat, god, Sesin, Changza, Shouo, white paper, windbreak, Sasam, Astragalus, Gyeji, Dansam, Soja, Pogongyoung, Shiho, fivefold, self It can be prepared by adding one or more herbal medicines selected from Kwak Hyang, and they can be mixed in a weight ratio of 1: 0.1 to 1, respectively.

In the present invention, the effective amount of the herbal extract expected to improve the symptoms of allergic diseases is 0.5g to 4.0g per day for adults (60kg body weight), preferably 1.5g to 3.0g per day, added to various foods The herbal extract of the present invention is suitably 5 to 80% by weight of the total weight, preferably 20 to 70% by weight of the total weight is preferred, more preferably 40 to 60% by weight.

And the present invention can be prepared by a variety of foods by a conventionally known method by adding the herbal extract obtained in the conventional food, it can also be formulated in a general formulation.

The herbal extract of the present invention is suitable for use as a food result of acute toxicity test.

Hereinafter, the present invention will be described in detail by production examples and experimental examples.

The following examples are intended to illustrate the invention and are not intended to limit the scope of the invention.

Preparation Example 1 Preparation of Herbal Extracts According to the Present Invention

Eui-in 150g, 150g of lettuce, 150g, Eoseongcho 112g, Gilgyeong 112g, raw yellow sulfur 75g, gold and silver 75g, triticale 75g, ginger 75g, dermis 75g, white extract 75g, creation 75g, Astragalus 75g, licorice 75g, peppermint 60g, red bean 37g, leek 800g and dried jujube 50 g and 2 g of the legal seed powder heated for 12 hours at 1000 ° C. or more were mixed, and distilled water was added thereto and gradually heated at 80 ° C. for 4 hours to give a final volume of 6,000 ml, which was then concentrated under reduced pressure and spray dried. 500 g of powder were obtained.

Preparation Example 2 Preparation of Physiological Isotonic Solution

100 ml of physiological saline was added to the herbal powder prepared in <Preparation Example 1>, and the pH was adjusted to pH 7.0 with 20% NaOH solution, and then stored at 4 ° C. or less for 2 hours to settle the inorganic salts, followed by a milipore filter (pore size 1.2). Μm), and the filtrate was put in a 20ml vial bottle and placed in a high-pressure sterilizer and autoclaved at 2 atm 120 ° C for about 30 minutes.

Experimental Example 1 Evaluation of the inhibitory effect of the production of lipid peroxide

The method was applied to the method of Kiso, that is, 100 μl of rat liver microsome suspension (20mg of protein / ml) in 500 μl of trihydroxymethylaminomethane (Tris) -HCl buffer (167mM KCl, 74.4mM Tris, pH7.4). 100 μl of the sample solution adjusted to each concentration with 100 μl of 2 mM NADPH, 100 μl of 10 mM ADP and 10% N, N-dimethylformamide (DMF) solution was added thereto, and warmed at 37 ° C. for 5 minutes. Then, 100 μl of 100 μM FeCI3 was added and warmed at 37 ° C. for 20 minutes.

For control, 100 μl of 10% DMF solution was added instead of sample solution, and 300 μl of water instead of 100 μl of NADPH, 100 μl of FeCI3, and 100 μl of ADP. After stopping the reaction by ice cooling, the mass of fat peroxide was calculated as the amount of malondialdehyde according to the method of Ohkawa et al. In other words, 200 ml of 8.1% sodium dodecyl sulfate (SDS) aqueous solution and acetic acid buffer solution (adjusted 20% AcOH containing 0.27 M HCl to pH 3.5 with 10 M NaOH) were added to 1 ml of the reaction solution. Heated for 20 minutes. After heating, the mixture was stopped by ice-cooling and the mixture was finely mixed with 4 ml of n-BuOH-pyridine mixture (15: 1).

Thereafter, after centrifugation at 800 x g for 10 minutes, the absorbance at the surface liquid 532 nm was measured, and the results are shown in FIG. 1. The herbal composition according to the present invention exhibited a concentration-dependent inhibitory effect on ADP / Fe3 + induced lipid peroxidation reaction using rat liver microsomes, and showed a stronger inhibitory effect than the positive control BHT.

Experimental Example 2> Evaluation of antiallergic action (PCA method) in oral administration of rat

The antiallergic action of the pharmaceutical compositions of the present invention was evaluated using the dermal anaphylactic reaction (PCA reaction) of rat oral antiallergic drug tranilast. Koda et al. Int. Arch. Allergy Appl. Immunol. 87, 254 (1988). Shave the back of Wister male rats weighing about 200 grams, dilute antiserum with homologous PCA titers of 1: 128-1: 256 with physiological saline for 48 hours, and inject 0.1ml intradermally into the back. It was. After 48 hours, 1 ml of 0.5% Evans blue saline solution containing 1 mg of DNP-BSA was injected into the tail vein as a protein. After 30 minutes, the rats were bled and cut off the pigmented spots on the skin of the back to determine the amount of leaked pigment. In other words, the cut skin is placed in a test tube, and 1 ml of 1N KOH is added, and the pigment is eluted by leaving it at 37 ° C. overnight, and 9 ml of acetone and 0.6 N phosphate mixture (13: 5) are added to shake and insoluble matter ( After filtering off the filtrate, the absorbance of the filtrate was measured at 620 nm to measure the amount of pigment, and the degree of pigmentation of the foot was confirmed. The samples were suspended in 0.2% CMC-Na, adjusted to 0.5 ml dose per 100 g of rat body weight, and orally administered 2 hours before challenge.

As can be seen in Figure 2, the composition according to the present invention showed anti-allergic activity even in oral administration, showed a stronger activity than the control.

Experimental Example 3> Evaluation of 5-lipokishgenase Inhibitory Activity

Blackham et al. Applied the method, namely, the cells were disrupted by sonicating RBL-1 cell suspension adjusted with 50mM phosphate buffer so that the cell number was 1 × 10 7 cells / ml. To 500 µl of this cell homogenate solution, 10 µl of sample solution adjusted to each concentration with 1% DMSO, 10 µl of 100 mM CaCl 2 and 10 µl of Memg solution of 10 mg / ml arachidonic acid were added at 37 ° C. Warm for 3 minutes. Control was given 10 μl of 1% DMSO instead of the sample. In addition, 10 µl of 1% DMSO was added to the blank and 500 µl of 50 mM phosphate buffer was added instead of the RBL-1 cell suspension. Thereafter, 500 µl of MeOH was added, the reaction was stopped by ice-cooling and mixed, and then centrifuged at 4 ° C and 2,000 x g for 15 minutes. Subsequently, the amount of 5-HETE in 10 µl of the surface liquid floated by HPLC was measured under the following conditions.

<HPLC condition>

column; Cosmosil 5C18 (4.6 x 150mm)

Mobile phase; acetonitrile: 0.1% acetic acid = 85: 15

Flow velocity; 1.0ml / min

Detection wavelength; 235 nm

Inhibition rate for 5-lipokishgenase activity was calculated by the following formula.

% Inhibition = ((sample-blank) × 100) ÷ (control-blank) =

In FIG. 3, the pharmaceutical composition of the present invention has an inhibitory activity (IC50) of about 18 pg / ml on 5-lipokinase, and showed stronger inhibitory activity than that of the positive compound BHT (IC50 = 28 pg / ml).

Experimental Example 4> Evaluation of Antihistamine Activity

The antihistamine action by the Magnus method using the Mormoto tube of the herbal composition of this invention which positively controlled diphenhydramine hydrochloride was tested. The mortar, which has been fasted overnight, is smashed, the ileum is removed, cut to a length of about 2 cm, one end is hanged on the isotonic transducer, and the other end is fixed to the fixed rod. And maintaining the temperature of the constant temperature bath (恒溫槽) in 37 ℃, and then, while breathable (通氣) a mixed gas of _{O 2 95%, CO 2 5} % in the Tyrode solution of 10㎖ allowed to stand for about 30 minutes, histamine for each concentration Injected, washed and trained, the same values for histamine were calculated under sample administration. As a result, as shown in FIG. 4, the antihistamine effect of the pharmaceutical composition of the present invention was about 2 to 4 times stronger than the control group.

Experimental Example 5> Specific immunomodulatory ability test of trinitrophenyl (TNP)

Trinitrobenzenesulfonic acid was dissolved in phosphate buffered physiological saline (35 mg / 7.0 mL, pH 7.5) and dropped therein with 1 mL of quantitative blood cell ferret. It was left to stand at room temperature for 30 minutes while mixing several times in a light shielding state, and then washed twice with physiological saline. After centrifugation at 3,000 rpm for 5 minutes, 5 × 109 TNP-SRBCs were intraperitoneally administered to 6-week-old female BALB / c mice to prepare 5 × 109 / ml with saline. Anti-TNP antibodies were measured by enzyme-linked immunoassay (ELISA) using dinitrophenyl bovine serum albumin (DNP-BSA). As a result, strong antibody production of anti-TNP-IgM and anti-TNP-IgG was confirmed as the peak at day 7. In addition, almost no type of antibody production was observed in BALB / c nude mice lacking thymus. Anti-TNP by oral administration for 7 days from the TNP-SRBC sensitized day in histamine gamma-globulin (200 mg / kg / day) for this mysterious war dissolved in physiological saline using the experimental system described above. The effect on antibody production was investigated. In addition, as a positive control, histamine gamma γ-globulin (200 mg / kg / day) was compared by subcutaneous injection in the same form. The result in this experiment example is shown in FIG. In addition, in the following experiment result, the meaningful difference of the mean value with a control was calculated | required by * using the Student's t-test. (*: p <0.05, **: p <0.005)

Experimental Example 6 Evaluation of Eosinophil Growth Inhibitory Activity

BALB / c mice, 6-week-old females, were orally administered 30 mg / day of the pharmaceutical composition according to the present invention, and 100 μl of Hogweed pollen fluid diluted 1,000-fold with physiological saline was 100 μl and 100 μl on the first day. Was subcutaneously injected on days 7 and 14. On day 21, 100 μl of 1,000-fold diluted hogweed antigen was injected intraperitoneally into the mouse and the reaction was induced. Peritoneal leached cells were harvested 24 hours after induction, and total cell number, eosinophil count, neutrophil count, and monocyte count were measured by laver staining. As a result, as shown in FIG. 6, the eosinophil count in the control group was the highest on the 27th day after induction, but the experimental group showed about 4 times the inhibitory effect than the control group on the 27th day of the 50 mg / Kg treatment group. Moreover, in BALB / c nude mice lacking T cells, eosinophils did not recognize any intraperitoneal infiltration.

Experimental Example 7> Nitric Oxide (NO) Production Inhibition Effect Experiment

In order to assay the allergic rhinitis efficacy of the herbal composition according to the present invention, the rhinitis-induced rhizome was prepared in two groups of 5 rats per group according to the modification of Bellofiore, etc., and dissolved 1 mg of Ovalbumin (OA) in 1 ml of 0.9% saline on the first day. Subcutaneous injection and immunization were performed by intranasal injection of 1 ml of 0.9% saline containing 6 × 10 ⁹ B. pertussis bacilli . Allergic rhinitis by antigen sensitization was induced by inhalation of 0.9% saline aerosol containing 2% (wt / vol) OA in rats 14 days after systemic immunization. Aerosol was made by ultrasonic nebulizer and inhaled for 20 minutes.

After 24 hours of rhinitis induction, the isotonic solution prepared in Production Example 2 was added thereto.

10 rats were anesthetized with chloroform, cardiac punctured, and the blood was poured into a test tube containing EDTA (Ethylene Diamine Tetraaccetic Acid Dipotassium Salt), mixed well to prevent coagulation, and centrifuged at 3000 rpm for 5 minutes using a centrifuge. To confirm NO production, transfer 50µl to 96well and process 50µl of Griess reagent (1% sulphanilamide, 0.1% naphlethyendiamine dihydrochloride, 4% phosphoric acod), which is a color development reagent, and measure NO production at 570nm with ELISA reader. It was.

Table 1 NO Production of Ovalbumin-Induced Rhinitis Rats

sample NO generation (μM / well) Normal 4.0 ± 1.82 Control 78.2 ± 2.9 * Experimental group 23.1 ± 3.5

* p <0.05

As a result, the NO production of the normal group was 4.0 ± 1.82 μM, the control group was about 78.2 ± 2.9 μM, and the treatment group of the composition of the present invention was 23.1 ± 3.5 μM, which showed a significant difference from the control group.

From these results, the herbal composition according to the present invention is nitric oxide (nitric), an inflammation-inducing substance known to cause inflammatory diseases such as arthritis, sepsis and tissue transplant rejection, autoimmune diseases, immune diseases such as diabetes, and neuronal cell death. It can be seen that there is an effect that suppresses the production of oxide, NO).

Experimental Example 8> Inflammatory Response Test of Rhinitis and Bronchus

After inhaling OA aerosol in the experimental animals as in <Experiment 7>, bronchial washing was performed the next day.

4 ml of PBS (Phosphate buffered saline; Sodium Chloride 8g, Pottassium Chloride 0.2g, Disodium Hydrogen Phosphate 1.15g, Calcium Chloride 0.0005g, Magnesium Chloride 0.0005g) containing 0.1% EDTA 2Na was washed three times before BALF was washed. Got it. The volume was approximately 80% of the original volume and the total cell number was measured using a hemocytometer. The results are shown in Table 2 below.

Lymphocyte counts were added to lymphoprep (1.077 ± 0.0001 g / ml, Nycomed PharmaAs, Oslo, Norway) in BALF, centrifuged at 2,000 rpm for 25 minutes, discarded the upper layer, and separated white lymphocytes. After centrifuged for 3 minutes at 200 × g for 10 minutes, the cells were suspended in RPMI medium and the number of cells was measured by Trypan blue exclusion using an optical microscope. The results are shown in FIGS. 7 and 8.

TABLE 2

sample Cell number (× 10 ⁵ / ml) Normal 6.34 ± 1.25 Control 27.43 ± 1.53 Experimental group 10.63 ± 0.34

From the above results, the total cell count in the bronchial lavage fluid was 6.34 ± 1.25 × 10 ⁵ / ml in the normal group and 27.43 ± 1.53 × 10 ⁵ / ml in the control group, but in the experimental group treated with the composition of the present invention, 10.63 ± 0.34 × 10 ⁵ / Ml can be seen to show a significant reduction compared to the control.

In addition, lymphocyte number is the normal group was 2.23 ± 1.45 × 10 ⁵ / ㎖ and the control group was approximately as compared with the control group as 14.53 ± 1.01 × 10 yieoteuna ⁵ / ㎖, the experimental group treated with the composition of the invention 5.71 ± 2.45 × 10 ⁵ / ㎖ It can be seen that the reduction by 60%.

Experimental Example 9> Confirmation of Increase of CD4 + / CD8 + Number of T Cells

Lymphocyte cells suspended in RPMI medium for T-cell measurement in airway lavage fluid were resuspended in media A (pH 7.2 PBS + 5% FBS with RPMI + 2M sodium azide) at 2 × 10 ⁷ cells / mL cell count. 50 μl of cell suspension was added to the cells so that 1 × 10 ⁶ cells were present in each test tube. Add 0.5 μg of FITC Anti-Rat CD4 + Monoclonal Antibody (Cedarlane, Ontario, Canada) and PE Anti-Rat CD8 + Monoclonal Antibody (Cedarlane, Ontario, Canada) to each test tube, mix well with Vortex mixer, and mix the mixture with light. Cover with aluminum foil, incubate for 30 minutes at 4 ° C, wash twice with PBS at 4 ° C, and cell pellet in 50ul ice cold media B (pH 7.2 PBS + 0.5% Bovine serum albumin + 2M sodium azide). After resuspending, CD4 + and CD8 + T cell rates for lymphocytes were measured using a fluorescence microscope. The cell number was calculated by multiplying the calculated lymphocytes by the ratio of CD4 + and CD8 +.

TABLE 3

sample CD4 + (× 10 ⁴ / ml) CD8 + (× 10 ⁴ / ml) Normal 1.34 ± 0.21 1.12 ± 0.52 Control 6.13 ± 1.53 5.02 ± 0.56 Experimental group 3.65 ± 2.34 1.62 ± 0.75

From the above experimental results, the number of CD4 + lymphocytes in airway lavage was 1.34 ± 0.21 × 10 ⁴ / ml in the normal group and 6.13 ± 1.53 × 10 ⁴ / ml in the control group, but 3.65 ± in the experimental group treated with the herbal composition of the present invention. 2.34 × 10 ⁴ / mL significantly reduced the number of CD4 + derived from Th2 compared to the control group, and CD8 + lymphocyte count was 1.12 ± 0.52 × 10 ⁴ / mL in the normal group and 5.02 ± 0.56 × 10 ^{4 in the} control group. / Ml, but the experimental group treated with the herbal composition of the present invention is 1.62 ± 0.75 × 10 ⁴ / ㎖ can be seen that the effect of about 62% decrease compared to the control group.

Therefore, it can be seen that the herbal composition according to the present invention is effective in suppressing the increase of CD4 + and CD8 + of T lymphocytes caused by non-saline.

Experimental Example 10> Confirmation of Reduction of T Cells in Blood

In a medium containing penicilin / streptomycin in 5% RPMI medium, 10 ⁴ cells were dispensed in a 6 well culture dish containing a slim cover glass and incubated at 37 ° C. for 24 hours, in Preparation Example 1 of the present invention. The reaction was observed by adding the prepared pharmaceutical composition to 50 ㎍ / ml, and after removing the waste solution, the cells were washed with PBS once, and the cells were fixed with metanol on the cells, and then washed with PBS and then run three times After blocking with 0.2% BSA (bovine serum albumin), the first antibody, Anti-mouse-CD4 + / CD8 +, was labeled with a second antibody, anti-mouse-conjugated FITC solution, and then shaded with foil. It was left.

This was observed under a fluorescence microscope. As a result, as shown in FIG. 9, the normal group showed a weak response, but the control group showed a tendency to increase the number of strong green fluorescence, whereas the fluorescence expression was significantly decreased in the experimental group and was much higher than the control group. It can be seen that there is a tendency to decrease.

The herbal extracts according to the present invention can be prepared directly by tablets, granules or soft capsules, and can be added to various foods such as tea and beverages to produce functional foods to have an allergic disease symptom.

Example 1 Preparation of Beverages

20% by weight of the herbal extract powder prepared in Preparation Example 1, 0.00075% by weight of vitamin B ₁ hydrochloride, 0.00075% by weight of sodium vitamin B ₂ phosphate, 0.00045% by weight of vitamin B ₆ hydrochloride, 0.00005% by weight of folic acid, 0.003% by weight of nicotinamide, The mixture was filtered by mixing 0.095% by weight citric acid, 25.3% by weight sugar and 54.6% by weight purified water, and the mixture was sterilized and filled for 20 seconds at 90 ° C or higher, and then sterilized for 30 minutes at 85 ° C.

Example 2 Preparation of Tablets

500 mg of powder prepared in Example 1, 100 mg of lactose and 100 mg of starch were mixed and tableted according to a conventional method for preparing tablets.

Example 3 Preparation of Granules

The crude drug extract powder prepared in Preparation Example 2, 35% by weight of vitamin C, 0.3% by weight of glucose, 28% by weight of glucose, 28% by weight of dextrin, 8.7% by weight of 70% ethanol were kneaded together and passed through a granulation machine to prepare granules of 60mesh granules. It was.

Example 4 Preparation of Capsules

500 mg of water extract prepared by Example 1, 50 mg of lactose, 50 mg of starch, 2 mg of talc were mixed and the capsules were prepared by filling gelatin capsules according to a conventional method for preparing capsules.

As described above, the present invention contains herbal medicines of Euiin, baekbaekpi, Eoseongcho, Gilgyeong, rawjihwang, gold and silver, three hundredth, ginger, dermis, baekchul, creation, Astragalus, licorice, peppermint, red pepper, green onion, jujube and medicinal herbs as main ingredients. Provides a functional food that can improve the symptoms of allergic diseases, functional foods having an active function to improve the symptoms of allergic diseases according to the present invention can be used regardless of the cause and constitution, digestive disorders, dizziness, even if used continuously for a long time And it is an invention having the characteristic that there is no side effect, such as heartburn.

1 is a graph showing the evaluation of the inhibitory action of lipid peroxide (過 酸化 脂質) production on the herbal composition according to the present invention,

2 is a graph showing the evaluation of antiallergic action (PCA method) in oral administration of rats to the herbal composition according to the present invention,

Figure 3 is a graph showing the evaluation of 5-lipokishinase inhibitory effect on the herbal composition according to the present invention,

Figure 4 is a graph showing the evaluation of anti-histamine action on the herbal composition according to the present invention,

5 is a graph showing a specific immunomodulatory capacity test of trinitrophenyl (TNP) for the herbal composition according to the present invention,

6 is a graph showing the eosinophil increase inhibition experiment for the herbal composition according to the present invention,

Figure 7 is a graph showing the number of lymphatic bronchial lymphocytes in the rhinitis induced rats for herbal composition according to the present invention,

FIG. 8 is a microscopic magnification (× 200) of morphologically showing tryphan blue staining of lymphocytes in pulmonary bronchus for a herbal composition according to the present invention; FIG.

9 is an enlarged fluorescence microscope (× 200) shown by the immunocytochemical analysis of pulmonary bronchioles for herbal compositions according to the present invention.

Claims (4)

For Euiin, the weight ratio of Sangbaekpi, Eoseongcho, Gilgyeong, Saenghwanghwang, Geumhwa, Sambaekcho, Ginger, Dermis, Baekchul, Creation, Astragalus, Licorice, Peppermint, Red Rice, Green onion, Jujube and Moji 1: 0.7 ~ 1: 0.7 ~ 1: 0.7 ~ 1: 0.1 ~ 1: 0.1 ~ 1: 0.1 ~ 1: 0.1 ~ 1: 0.1 ~ 1: 0.1 ~ 1: 0.1 ~ 1: 0.1 ~ 1: 0.1 ~ 1: 0.1 ~ 1: 0.1 ~ 1: 1 ~ 10: 0.1 ~ 1: 0.01 ~ 0.1 Food additive for improving allergic disease symptom containing herbal extract prepared as an active ingredient. Functional food having an allergic disease symptom improving activity, comprising 5 to 80% by weight of the food additive according to claim 1 relative to the total weight According to claim 2, wherein the food is a functional food having an allergic disease symptom improving activity, characterized in that selected from tablets, granules, soft capsules or liquid formulations According to claim 2, wherein the functional food is a functional food having an allergic disease symptoms improvement activity, characterized in that the beverage or tea.