KR100501309B1 - Manufacturing Method of Nano-emulsified Cosmetic Composition Containing Arbutin - Google Patents

Abstract

The present invention relates to an arbutin-containing nanoemulsion cosmetic composition having a significantly improved transdermal absorption rate by minimizing the size of emulsified particles to a nanoparticle size in a cosmetic composition containing emulsified arbutin, which is a whitening active material, and a method for preparing the same.

Specifically, the present invention separates 1-30% by weight of the oily ingredient mixture based on the final cosmetic composition, and 1-3% by weight of arbutin and the aqueous phase mixture containing 0.5-5.0% by weight of hydrogenated lecithin as emulsifiers. After dissolving in the dissolution tank, the dissolved oil phase mixture is added to the aqueous phase mixture and premixed by premixing with a homomixer or paddle mixer. Finally, the first preliminary composition is placed in a microfluidizer and 200 A method for producing an arbutin-containing nanoemulsified cosmetic composition, comprising a second emulsification at -2000 atmospheres, and a cosmetic composition produced thereby.

Description

Manufacturing Method of Nano-emulsified Cosmetic Composition Containing Arbutin

The present invention relates to a cosmetic composition comprising emulsifying the whitening active material arbutin to nanoparticle size, and relates to an arbutin-containing whitening cosmetic composition having a significantly improved transdermal absorption by minimizing the size of emulsified particles, and a method for preparing the same.

Arbutin is a skin whitening agent derived from bilberry leaves and has a relatively low irritation, and thus is widely used for skin de-pigmentation.

Arbutin has a structure in which β-D-glucose is bound to hydroquinone, and when applied to skin, β-D-glucose is slowly degraded by a degrading enzyme present in the human body, and thus hydroquinone, a bioactive substance. It is known to exhibit the outstanding whitening effect by this slow decomposition.

As an example of using the whitening function of arbutin, Korean Patent Publication No. 2001-0109730 discloses an example of preparing a whitening cosmetic using a bilberry containing arbutin.

However, such arbutin has been limited in its use because it has an excellent whitening effect and has a disadvantage of causing severe discoloration when formulated into cosmetics, and many studies have been conducted to solve this disadvantage.

As one example of such studies, Korean Patent Publication No. 1999-0065147 discloses an attempt to stabilize arbutin, an active ingredient, by containing rutin (C ₂₇ H ₃₀ O ₁₆ ) together with arbutin, In Korea Patent Registration No. 10-0198508, attempts have been made to obtain stability of arbutin-containing whitening cosmetics by encapsulating arbutin using polylactic acid (PLA) as a wall material.

However, all of these attempts have their own disadvantages. As mentioned above, there is still a problem of discoloration in general cosmetics containing only arbutin without any safeguards, and other stabilization such as routines to ensure safety. In the case of adding the ingredients, there may be negative effects of the added stabilizer on the cosmetics, and in the case of encapsulation, the manufacturing process may be complicated, thereby increasing the manufacturing cost of cosmetics and not guaranteeing the whitening efficacy of arbutin, which is an active ingredient. have.

Skin, which is composed of epidermis, dermis and subcutaneous fat, is the largest organ in the human body and plays various roles such as protective function, barrier function, temperature control function, excretory function and respiratory function. The thickness of the epidermis and the dermis varies depending on the site, but is about 2-5mm, and the thickness of the subcutaneous fat varies greatly among people.

The epidermis on the outer side of the dermis is only about 0.1mm thick, mainly made of skin cells called keratinocytes, and there are scattered pigmented cells that make brown pigment and cells related to skin immunity. .

The epidermis is further divided into the basal layer, the pole layer, the granule layer, and the stratum corneum. The keratinocytes constituting the epidermis are generated from the basal layer, and the granule layer in which the granules are formed in the cells when viewed under a microscope. In the outermost part of the skin, the keratin fibers and granules form a stratum corneum composed of dead cells in a tightly coupled state.

The stratum corneum, which consists of 10-20 layers of flat keratinocytes, may contain water that is several times the dry weight when hydrated and has a thickness of about 10 μm. When applied to cosmetics, such as to act as the largest barrier (barrier).

The percentage of cell gap in the stratum corneum is 10-30%, which is larger than the percentage of cell gap in normal tissues, and is filled with multiple layers of lipid bilayer, which plays an important role in the function of the stratum corneum.

Because of the barrier function of the skin, especially the stratum corneum, transdermal absorption is one of the most important considerations in the development of therapeutic drugs and cosmetics, and in recent years, it is possible to test the absorption of physiologically active ingredients in skin due to the improvement of skin cell culture technology. Various in vitro assays have been developed, which have greatly helped the development of drugs and cosmetics with high transdermal absorption.

Specifically, according to Korean Patent Registration No. 10-0256781, by efficiently orienting a higher alcohol and a surfactant to an interface together with a certain amount of oil, to achieve emulsion stabilization and improvement of transdermal absorption quickness through Brownian movement of emulsified particles. Attempts have been made.

In addition, according to Korean Patent Publication No. 2000-0040042, ceramide, nonionic surfactant, higher alcohol, cholesterol, and oil are added to a nonaqueous solvent, mixed, heated, dissolved, and ascorbic acid as an active ingredient. Attempts have been made to promote transdermal absorption of cosmetic compositions by adding them, dissolving them, emulsifying them at high speed, and cooling them to prepare a cosmetic composition in the form of a liquid crystal gel.

In addition, Korean Patent Publication No. 2002-0008598 discloses plum extract, carrot extract in liposomes prepared using lecithin, ethanol, ester oil, propylene glycol, cholesterol, beta-sitosterol, ceramide, vegetable oil, and the like. Attempts have been made to improve the percutaneous absorption rate by encapsulating the active ingredient such as by stabilizing the active ingredient of the cosmetic composition and then absorbing it into a nonwoven fabric.

However, such attempts, namely, methods for promoting surface activity, methods using liquid crystal gel formulations, and methods for preparing cosmetics in the form of liposomes are complicated or limited to the content of arbutin, an active ingredient. The lack of a whitening effect, or liposomes are easily broken, or had the disadvantages such as the lack of arbutin percutaneous absorption enhancement effect originally.

As described above, in containing arbutin as a whitening ingredient in cosmetics, it is possible to improve the whitening effect and greatly improve the percutaneous absorption effect of arbutin by increasing the content of arbutin as an active ingredient without adding an additional stabilizer. In addition, the manufacturing process is simple, the manufacturing cost of the low cost arbutin-containing cosmetic composition and the appearance of the cosmetic composition prepared by using the situation is urgently needed.

The present invention is to overcome the problems of the prior art, the purpose is to increase the content of arbutin as an active ingredient without adding an additional stabilizer to promote the whitening effect of arbutin and greatly improve the transdermal absorption effect. The present invention is to provide a arbutin-containing nanoemulsified cosmetic composition and a manufacturing method thereof having a low manufacturing cost due to a simple manufacturing process.

To this end, the present invention, 1-30% by weight of the oil-based mixture based on the final cosmetic composition, and 1-3% by weight of arbutin and a water-based mixture containing 0.5-5.0% by weight of hydrogenated lecithin as emulsifiers are separately dissolved After dissolving in the mixture, the dissolved oil phase mixture was added to the aqueous phase mixture and premixed with a homomixer or paddle mixer, and preliminarily pre-emulsified. Finally, the first preliminary composition was placed in a microfluidizer. It provides a method for producing an arbutin-containing nanoemulsified cosmetic composition, and an arbutin-containing nanoemulsified cosmetic composition produced thereby, which is emulsified secondary at 2000 atmospheres.

The present invention relates to an arbutin-containing nanoemulsified whitening cosmetic composition of which the percutaneous absorption rate of arbutin is greatly improved by minimizing the size of the emulsified particles to nanoparticle size in the cosmetic composition containing emulsified arbutin as a whitening active material. Specifically, the present invention relates to an arbutin-containing nanoemulsified whitening cosmetic composition using hydrogenated lecithin as an emulsifier and emulsified at high pressure using a microfluidizer.

Conventional cosmetic arbutin-containing emulsion composition is a water-soluble polymer as a thickener for increasing stability with a nonionic surfactant and a water-oil component having a relatively low skin irritation, which is homogenized by a homomixer with arbutin Prepared by the method, the content of each component was appropriately adjusted according to the viscosity or the desired usability.

However, in the case of the emulsion composition prepared by emulsifying with a homomixer, it is difficult to make small and fine emulsion particles and it is difficult to apply to low viscosity emulsion products, so the applicant uses a microfluidizer instead of a homomixer. When the microfluidizer is applied to a microfluidizer using lecithin, especially hydrogenated saturated lecithin, as an emulsifier, it is possible to obtain fine and uniform emulsion particles at the nanoparticle level. As a result, the present invention has been found to significantly improve the percutaneous absorption rate of arbutin and the whitening effect thereof.

Microfluidizer is a machine that performs emulsification using high pressure. It is generally used to emulsify the emulsion firstly emulsified by homomixer. It is a machine that uses the principle of making emulsified particles fine by cavitation and collision force caused by pressure fluctuation that occurs when it flows out.

When the emulsion is prepared, it is known that the emulsification capacity of the microfluidizer is about 10,000 times larger than that of a general homomixer.

Lecithin (lecithin) used as an emulsifier in the present invention is one of the main phospholipids constituting the cell membrane system of the animal, also called phosphatidyl choline (phosphatidyl choline), as shown in Equation 1 below, such as phosphoric acid and choline It is a substance which has a surface activity because it has the structure which the hydrophilic component couple | bonded and the other side hydrophobic acyl group couple | bonded.

............. Equation 1

Wherein R and R 'are acyl groups of C ₁₄ -C ₂₂

In this study, hydrogenated lecithin (HLB 8-9) saturated with unsaturated fatty acids in lecithin molecules was used. In the case of hydrogenated lecithin PC (phosphatidyl choline), since the critical packing parameter value is 1/2 to 1, a critical packing shape forms a vesicle with a truncated cone. It is thought that the nanoemulsion can be prepared by using a microfluidizer emulsifying device that can give a high shear to the emulsification system using the geometry of the hydrogenated lecithin molecules.

Such lecithin has a composition similar to the lipid component of the skin and thus has high skin affinity, thereby promoting skin permeability of the stratum corneum during the transdermal absorption of cosmetics.

Hereinafter, the preparation method of the arbutin-containing nanoemulsion cosmetic composition of the present invention using hydrogenated lecithin as a microfluidizer and an emulsifier will be described in detail.

First, a homomixer or paddle mixer in a dissolution tank, each containing 1-30% by weight of an oily mixture based on the final cosmetic composition, and 1-3% by weight of arbutin and 0.5-5.0% by weight of hydrogenated lecithin as an emulsifier, respectively. Mix and dissolve.

Next, the dissolved oil phase mixture is added to the aqueous phase mixture and preliminarily pre-emulsified with a homomixer or paddle mixer.

Finally, the primary emulsion composition is placed in a microfluidizer (Model M-110EHI, Microfluidics, USA) to emulsify secondary at 200-2000 atm to prepare a final arbutin-containing emulsion composition.

According to this method, the emulsion composition emulsified in the oil-water (oil-in-water) phase in the homomixer is secondly through the microfluidizer to be a fine and stable nanoemulsion composition.

The nanoemulsified composition thus prepared is composed of fine and uniform particles having an average particle size of 100-400 nanometers and having a viscosity of 1 cps to 5000 cps, and thus the quality of the formulation is excellent and the transdermal absorption rate of arbutin and the whitening effect thereof. Will be able to provide a greatly improved cosmetics.

In the present invention, as the oil phase component, vegetable squalane, phenyltrimethicone, cyclopentasiloxane, cetyloctanoate, meadowfoam seed oil, dimethicone, tocopheryl acetate, and beeswax are used.

In the present invention, glycerin, arbutin, triethanolamine, trisodium idieti, dipropylene glycol and hydrogenated lecithin are used as the aqueous phase component.

Examples and comparative examples and test examples using the same to describe the present invention in more detail, but the present invention is not intended to be limited thereto.

EXAMPLE

As an embodiment of the present invention, according to the composition of Table 1 below, to prepare a cosmetic composition Examples 1-3 in the form of a skin, lotion, cream. The units in Table 1 are by weight.

No. ingredient Example 1 Example 2 Example 3 One  Vegetable squalane 6.0 6.0 6.0 2  Phenyltrimethicone 3.0 3.0 3.0 3  Cyclopentasiloxane 2.0 5.0 4.0 4  Cetyloctanoate - 2.0 5.0 5  Meadowfoam seed oil 0.1 1.0 2.0 6  Dimethicone - - 0.5 7  Tocopheryl Acetate 0.1 0.1 0.1 8  Beeswax - - 0.5 9  glycerin 4.0 4.0 4.0 10  Arbutin 2.0 2.0 2.0 11  Triethanolamine - 0.2 0.3 12  Trisodium IDT 0.01 0.02 0.02 13  Dipropylene glycol 5.0 5.0 5.0 14  Hydrogenated Lecithin 1.0 1.0 1.0 15  Carboxy Vinyl Polymer - 0.2 0.3 16  ethanol 3.0 3.0 - 17  antiseptic q.s q.s q.s 18  incense q.s q.s q.s 19  Pigment q.s q.s q.s 20  Purified water To 100 To 100 To 100

Manufacturing method

1) Arbutin as an active ingredient, hydrogenated lecithin as an emulsifier, and water phase components, that is, components 9-14 and purified water, are added to a water phase dissolution tank, heated to 70-75 ° C, mixed and dissolved in a paddle mixer.

2) The oily component, i.e., components 1-8, is added to a separate dissolution tank, warmed to 70-75 ° C and completely dissolved.

3) The oil phase component of 2) in the state of 70 to 75 ° C. is introduced into the aqueous phase dissolution tank of 1), mixed with a homomixer or paddle mixer for 5 minutes and preliminarily emulsified.

4) The primary emulsion prepared in 3) is added to a microfluidizer, treated once at a pressure of 500 bar, cooled to 45 ° C., and then additives, that is, components 15-19, are mixed and mixed. After the degassing, it is terminated at 30 ° C.

Thus prepared Example 1-3 using a particle sizer (Particle Sizer Model 370, Nicomp, USA) to measure the size of the emulsified particles 30 times for each Example and the average value of the results are shown in Table 2 below . The units in Table 2 are nanometers (nm).

Item Example 1 Example 2 Example 3 Average particle size 302 330 338

The particle size of the emulsion composition prepared according to Examples 1-3 of the present invention is from 302 to 338 nanometers, compared to the emulsion composition prepared by the homomixer, which is very small in size, specifically, nano-level fine emulsion particles were prepared. Able to know.

[Comparative Example]

As a comparative example, according to the composition of Table 3, using only a homomixer, to prepare a cosmetic composition Comparative Examples 1-3 in the skin, lotion, cream formulation, respectively. The units in Table 3 are by weight.

No. ingredient Comparative Example 1 Comparative Example 2 Comparative Example 3 One  Vegetable squalane 6.0 6.0 6.0 2  Phenyltrimethicone 3.0 3.0 3.0 3  Cyclopentasiloxane 2.0 5.0 4.0 4  Cetyloctanoate - 2.0 5.0 5  Meadowfoam seed oil 0.1 1.0 2.0 6  Dimethicone - - 0.5 7  Tocopheryl Acetate 0.1 0.1 0.1 8  Beeswax - - 0.5 9  glycerin 4.0 4.0 4.0 10  Arbutin 2.0 2.0 2.0 11  Triethanolamine - 0.2 0.3 12  Trisodium IDT 0.01 0.02 0.02 13  Dipropylene glycol 5.0 5.0 5.0 14  Hydrogenated Lecithin 1.0 1.0 1.0 15  Carboxy Vinyl Polymer - 0.2 0.3 16  ethanol 3.0 3.0 - 17  antiseptic q.s q.s q.s 18  incense q.s q.s q.s 19  Pigment q.s q.s q.s 20  Purified water To 100 To 100 To 100

Manufacturing method

1) Arbutin as an active ingredient, hydrogenated lecithin as an emulsifier, and water phase components, that is, components 9-14 and purified water, are added to the main kiln, heated to 70-75 ° C, mixed and dissolved in a paddle mixer.

2) The oil phase component, i.e., components 1-8, is introduced into an oil phase dissolution tank and warmed to 70-75 ° C and completely dissolved.

3) The oil phase component of 2) in the state of 70-75 ° C. was added to the main kiln of 1) and emulsified at 3500 rpm for 3 minutes using a homomixer. It completes at 30 degreeC, stirring.

Thus Comparative Example 1-3 by using a particle sizer (Particle Sizer Model 370, Nicomp, USA) to measure the size of the emulsified particles 30 times for each comparative example and the average of the results are shown in Table 4 below. . The unit of Table 4 is micrometer.

Item Comparative Example 1 Comparative Example 2 Comparative Example 3 Average particle size 24.34 19.72 26.20

The particle size of the emulsion composition prepared according to Comparative Example 1-3 is 19.72 to 26.20㎛, compared to Example 1-3 produced by the microfluidizer, the emulsion particles having a very large size corresponding to about 100 times It can be seen that manufactured.

On the other hand, the particle size distribution of Examples 1-3 and Comparative Examples 1-3 of the present invention is shown graphically in FIG. By forming a narrower range than the comparative example was produced a more uniform particles it can be seen that it can increase the percutaneous absorption of arbutin.

Test Example 1 Safety Test

Patch tests were conducted to test the safety of the nanoemulsified compositions prepared in Examples 1-3 of the present invention. Eleven subjects participated in the test. Each patient was asked to apply 10 ㎍ of sample to each subject's arm, seal it with a patch, and then visually observe stimulation such as swelling after 48 hours. Table 5 shows.

Sample Erythema, itching Swelling No irritation Example 1 - One 10 Example 2 - - 11 Example 3 - - 11

As a result of the safety test, in Examples 2 and 3, there were no subjects showing symptoms of irritation or side effects. In Example 1, there was a slight irritation but disappeared after 2 hours. That is, it was shown that the example composition of the microemulsified particles prepared at high pressure using a microfluidizer improved the safety of arbutin.

Test Example 2 Whitening Effect Test

Example 2 and Comparative Example 2 were used to test the whitening effect.

Participants were 21 women in their late 20s to late 40s (mean age: 32.3 years).

1) Human test schedule and test method overview

The duration of each human subject was 6 weeks before the start of the test, after artificial pigmentation by UV irradiation (1 day after UV irradiation), after 1 week, after 2 weeks, after 4 weeks, and after 6 weeks. In addition, the use of whitening cosmetics and other cosmetics, which may affect the test results, was prohibited in addition to the experimental products provided, and procedures such as packs and massages were prohibited. In addition, a 1-mm-thick WG320 filter and UG5 filter were used on the upper and lower parts of the test site, and an ultraviolet-ray irradiator equipped with a 150W xenon arc lamp that removed UVC wavelengths and visible wavelengths (Multi-port Solar simulator 601, Solar Light, USA) irradiated with ultraviolet rays in a square of about 4 cm 2 size artificially induced pigmentation, and then the test subjects were given Experimental Example 2 and Comparative Example 2. The subject used Experimental Example 2 and Comparative Example 2, which were provided, after artificial pigmentation by ultraviolet irradiation (1 day after ultraviolet irradiation), twice a day every morning and evening, and after artificial pigmentation by ultraviolet irradiation (after ultraviolet irradiation). Day 1) After 1 week, after 2 weeks, after 4 weeks, and after 6 weeks, the melanometer index (N value) and Erythema Index (E value) were measured by Mexameter MX 18.

2) Mechanical evaluation of efficacy

① Skin brightness measurement by Chromameter CR-300

The skin whitening effect measured by Chromameter CR-300 (Minolta, Japan) was evaluated by measuring the change in skin brightness (L value) of the pigmented area.

② Measurement of Melanin Index and Erythema Index by Mexameter

The skin whitening effect measured by Mexameter MX 18 (Courage + Khazaka, Germany) was evaluated by measuring Melanin Index (N value) and Erythema Index (E value) of pigmented areas. The evaluation measured the L value of the Chromameter CR-300 in subjects after artificial pigmentation by UV irradiation (1 day after UV irradiation), after 1 week, after 2 weeks, after 4 weeks, and after 6 weeks. It was. In Table 6 below, L-Parameter values of Example 2 and Comparative Example 2 of the test subjects (21 persons) are shown as clinical results 3 days after artificial pigmentation by UV irradiation and 6 weeks after.

Subject Example 2 Comparative Example 2 3 days after artificial pigmentation 6 weeks after artificial pigmentation 3 days after artificial pigmentation 6 weeks after artificial pigmentation One 66.15 66.75 * 65.99 67.40 * 2 65.55 67.57 * 66.92 66.34 3 60.41 62.46 * 62.85 62.73 4 64.51 63.83 64.57 64.93 * 5 64.72 65.60 * 65.05 67.26 * 6 64.43 65.32 * 65.34 63.70 7 65.27 65.30 * 65.25 66.22 * 8 69.06 67.03 66.09 67.59 * 9 70.69 69.84 70.72 67.31 10 62.56 63.56 * 63.55 63.99 * 11 62.76 61.47 63.19 58.32 12 66.47 65.73 67.43 65.14 13 60.89 63.31 * 62.02 63.20 * 14 57.33 59.98 * 59.22 61.01 * 15 64.90 63.49 66.41 63.77 16 68.48 68.07 69.93 68.90 17 62.83 63.78 * 64.32 64.71 * 18 60.98 58.40 65.37 59.25 19 63.90 63.56 64.08 62.96 20 65.32 65.00 65.78 65.41 21 57.23 59.88 * 60.43 60.79 *

*: Whitening effect significant

(The higher the L-Parameter value, the brighter the skin tone.)

When the brightness of the skin was brightened by applying Example 2 and Comparative Example 2, which contained arbutin, a whitening active substance, during the clinical trial, at the value of the L-parameter of the skin after artificial pigmentation by UV irradiation in the whitening effect test. It was considered to have a whitening effect (when the value of L-Paramete was increased). Herein, the significance difference between Example 2 and Comparative Example 2 was determined by calculating the mean and standard deviation of the whitening effect.

The mean value of the value showing significance in Example 2 was 1.47, and the mean value of the value showing significance in Comparative Example 2 was 1.06, so that the whitening effect of Example 2 was high. As for standard deviation, Example 2 was 0.91 and Comparative Example 2 was 0.67. As a result, it was found that Example 2, which minimized the emulsified particle size of the cosmetic composition containing arbutin as the whitening active material to nanoparticle size, showed a higher whitening effect on the subjects.

Test Example 3 Percutaneous Absorption Test

The degree of transdermal absorption was tested using Examples 2, 3 and Comparative Examples 2, 3 of the present invention.

After 1 hour of application of each composition to the skin of hairlessmouse (6-8 weeks of age), an in vitro test was conducted to quantify the amount of the composition that penetrates percutaneously by HPLC. The arbutin content in the prepared samples is shown in Table 7 below, which was again diagrammed and shown in FIGS. 2A and B. The unit of the test result shown in Table 7 is microgram / ml, The specific test conditions of a percutaneous absorption test are as follows.

Transdermal Absorber: Variomac, 37 ° C, 800rpm, Frantz Cell

Dermal and Receptor: Hairlessmouse (6-8 weeks old), Solvent-PBS buffer (pH 7.0): Ethanol = 1: 1

Samples applied: 0.3 g per test, arbutin content 20 mg / ml

Sample after application: 0.03g per each composition

sample One 2 3 Average Transdermal residue Example 2 1.20 0.03 3.52 1.58 Comparative Example 2 3.78 3.26 1.75 2.93 Transdermal penetration Example 2 0.56 5.02 5.24 3.61 Comparative Example 2 3.18 1.97 1.78 2.28 Transdermal residue Example 3 3.31 2.74 2.79 2.95 Comparative Example 3 2.73 2.92 2.58 2.74 Transdermal penetration Example 3 5.21 4.29 4.43 4.64 Comparative Example 3 2.24 2.58 1.15 1.99

As can be seen from the above results, the microparticle emulsion composition of the present invention prepared using a microfluidizer had a higher percutaneous absorption rate than the comparative example prepared using a homomixer. In comparison with Comparative Example 2, the transdermal absorption rate was increased by 58%, and Example 3 was increased by 133% compared to Comparative Example 3.

The amount of arbutin remaining in the transdermal after application is lower than that of the present invention prepared using a microfluidizer compared to the comparative example, so that the amount of arbutin remaining in the transdermal is small, It can be said that the concentration of arbutin penetrating proves to have a high effect, that is, to significantly improve the percutaneous absorption rate of arbutin.

By completing the present invention as described above, it is possible to improve the whitening effect and greatly improve the percutaneous absorption effect of arbutin by increasing the content of arbutin as an active ingredient without adding an additional stabilizer, and the manufacturing process is simple and the manufacturing cost is low. Arbutin-containing nanoemulsified cosmetic composition and a method for preparing the same can be provided.

1 is a graph showing the particle size distribution of Examples and Comparative Examples of the present invention,

Figure 2a and b is a diagram showing the percutaneous absorption rate and transdermal absorption amount of the Examples and Comparative Examples of the present invention.

Claims (7)

In the manufacturing method of arbutin-containing nano-emulsion cosmetic composition, 1-30% by weight of the oily ingredients based on the final cosmetic composition was added to a dissolution tank, heated and dissolved at 70-75 ° C to prepare an oily mixture, Water phase components including 1-3% by weight of arbutin and 0.5-5.0% by weight of hydrogenated lecithin were added to a separate water melting tank, warmed and dissolved at 70-75 ° C. to prepare an aqueous phase mixture. A primary emulsion composition was prepared by adding the oil phase mixture in a 70-75 ° C. state to the aqueous phase mixture and premixing for 5 minutes. The primary emulsion composition is introduced into a microfluidizer and treated once at a pressure of 200-2000 atm, cooled to 45 ° C., and then added to an additive and cooled to 30 ° C. while stirring. doing, Method for producing arbutin-containing nano emulsified cosmetic composition. The method of claim 1, The oily ingredients are characterized by comprising vegetable squalane, phenyltrimethicone, cyclopentasiloxane, cetyloctanoate, meadowfoam seed oil, dimethicone, tocopheryl acetate, beeswax, Method for producing arbutin-containing nano emulsified cosmetic composition. The method of claim 1, The water phase component is characterized in that it comprises glycerin, arbutin, triethanolamine, trisodium ideti, dipropylene glycol, hydrogenated lecithin, Method for producing arbutin-containing nano emulsified cosmetic composition. delete delete delete delete