KR100495605B1 - Feed additive for broiler chicks and manufacturing method of that - Google Patents

Abstract

The present invention relates to a herbal feed additive for broilers and feed prepared by adding the same.

Preparation method of the herbal feed additive for broilers of the present invention, golden 10% by weight, 15% by weight of Seokchangpo, 10% by weight of saengchocho, safflower 30% by weight, wolfberry 10% by weight, airborne 10% by weight, glucose 15% by weight After mixing, pulverizing and mixing the remaining herbal medicine except glucose, and then drying the dried and prepared glucose together to make a solid in the form of a paste, granulating the solid and then drying to prepare a herbal feed additive for broilers It consists of doing.

In addition, by adding 0.8 ~ 1.2% by weight of the herbal feed additive of the present invention to the feed to prepare and feed broiler feed.

According to the present invention, Salmonella bacteria, which are intestinal bacteria of broiler chickens, are suppressed, weight gain, high growth rate, low mortality rate and low feed requirement are provided.

Description

Herbal feed additives for broilers and their manufacturing method {FEED ADDITIVE FOR BROILER CHICKS AND MANUFACTURING METHOD OF THAT}

The present invention relates to a herbal feed additive for broilers and a method of manufacturing the same. It also relates to feed for broilers prepared by the addition of herbal feed additives.

More specifically, it relates to a herbal feed additive for broilers prepared by adjusting the composition ratio of the gold, Seokchangpo, Eoseongcho, safflower, Gugija, Banggi herbal medicine by the pharmacological effect.

In the poultry industry, disease outbreaks, including Salmonella infections, are a problem.

As a countermeasure against Salmonella spp. In chickens, fructo- oligosaccharides, dried yeast, formic acid or proponic acid There have been reports of reduced ministerial settlements, but this has not been an effective way.

In addition, Salmonella's flagella has specificity that can be settled in the intestinal tract, and thus studies on vaccines and anti-infectious diseases have been conducted, but there are many vaccines, and there is a limit to vaccine prevention.

Korean Registered Patent Publication No. 10-0350093 (a feed additive for increasing physiological activity and promoting growth and development of cultured marine fishes using effective microorganisms and herbal medicines) and liquid molasses, green soybean milk, lactic acid bacteria, Bacillus, yeast and radiation bacteria Effective microbial complex fermentation broth containing, fermentation of photosynthetic bacteria, herbal extracts (ginseng, Angelica, Cheongung, Peony, Sukjihwang, Baekchul, Bokryeong, Astragalus, broiler, licorice, health, jujube, dermis, lychee fruit, vigor) Disclosed is a feed additive for physiological activity enhancement and growth development of marine fish.

In Korean Patent Publication No. 10-0348795 (functional fermented feed additive and preparation method thereof), water content is obtained by mixing calcium alumina silicate, calcium carbonate, zinc oxide, zinc sulfate, magnesium sulfate and magnesium oxide with rice bran or wheat skin. Disclosed is a method for producing a feed additive through a fermentation process by controlling it constantly.

Korean Laid-Open Patent Publication No. 2001-0011937 (Feed for promoting growth of livestock using herbal medicines) discloses a feed for promoting livestock growth and increasing feed efficiency by feeding alone or mixing Sanjoin and Seokchangpo in general livestock feed. It is.

However, these methods have a complicated method of preparing a feed additive and have a weak effect of reducing enterobacteria such as Salmonella bacteria in broilers.

The present invention to solve the above problems, to provide a feed additive for suppressing Salmonella (Salmonella) bacteria infection during broilers.

In addition, the present invention suppresses the intestinal pathogens of broilers to reduce the incidence of disease, increase the effect of the broiler, shorten the delivery period of broilers and provide a broiler feed containing broiler herbal feed additives that can contribute to farm income increase The purpose is to.

The present invention relates to a herbal feed additive for broilers and a method of manufacturing the same.

In addition, the present invention relates to a feed for broiler chicken broth added with a herbal feed additive for broilers.

Preparation method of the herbal feed additive for broilers of the present invention, golden 10% by weight, 15% by weight of Seokchangpo, 10% by weight of saengchocho, safflower 30% by weight, wolfberry 10% by weight, airborne 10% by weight, glucose 15% by weight After mixing, pulverizing and mixing the remaining herbal medicine except glucose, and then drying the dried and prepared glucose together to make a solid in the form of a paste, granulating the solid and then drying to prepare a herbal feed additive for broilers It consists of doing.

In addition, the composition ratio of each raw material at the time of manufacture of a herbal feed additive can be added or decreased within a normal range.

In addition, the herbal feed additive for broilers of the present invention is added to 0.8 ~ 1.2% by weight of the feed weight to prepare a feed for broiler feed. When the amount of the herbal feed additive is less than 0.8% by weight, the effect is weak, and when the amount is more than 1.2% by weight, the economy is inferior.

Looking at the main components and pharmacological effects of the feed additive of the present invention.

Major ingredients of Scutellariae Radix are baicalein and wagonin, which have strong antiviral activity and excellent antibacterial activity against typhoid bacteria and enteric bacteria.

The main components of Acori Graminei Rhizonin are beta-asalone (ß-asarone; C ₁₂ H ₁₆ O ₃ ) and caryophyllene (C ₁₅ H ₂₄ ), which reduce fat synthesis and have antiviral effects. It is effective in increasing immunity.

Houttuyniae Herba is the main ingredient of Decanoyl Acetaldehyde, Capric Acid ((CH ₃ (CH ₂ )) ₈ COOH), which has the effect of inhibiting fat synthesis and antibacterial activity. . The antibacterial action of Eochocho is broad, and it has antibacterial activity against E. coli, typhoid bacteria, paratyphoid bacteria, erythropoies, and anti-acid bacteria as well as non-pathogenic bacteria.

Safflower (Carthami Flos) is mainly composed of cartamine (C ₄₃ H ₄₂ O ₂₂ ), linoleic acid (CH ₃ (CH ₂ ) ₄ (CH = CH (H ₂ ) ₂ (CH ₂ ) ₆ COO) and the like, have an antioxidant effect, have anti-cancer effects (breast cancer, etc.), and have effects such as osteoporosis prevention.

Lgcii Fructus is composed of carotene (C ₄₀ H ₅₆ ), linoleic acid (CH ₃ (CH ₂ ) ₄ (CH = CH (H ₂ ) ₂ (CH ₂ ) ₆ COO), Vitamin B complex (Vit B complex), vitamin C (Vit C), and the like, cholesterol (Cholesterol) synthesis and suppression of the immune system is effective.

Banghi (Stephaniae Tetrandrae Radix) is the main component is flavonoids (Flavonoid), alkaloids (Alkaloid), etc., inhibits lipid synthesis and has an antioxidant effect.

In addition, the glucose used by mixing with the herbal medicine in the present invention has the effect of increasing the energy efficiency.

Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples, but these do not limit the scope of the present invention.

Example 1 Preparation of Herbal Feed Additives for Broiler Chickens

Gold, Seokpo, Eoseongcho, safflower, wolfberry, Banggi herb and glucose were purchased from the market and prepared.

1 kg of prepared gold, 1.5 kg of Seokchang-po, 1 kg of Ersungcho, 3 kg of safflower, 1 kg of wolfberry, and 1 kg of air permeation were blended.

The blend was ground to a fine powder using a grinder.

The pulverized product was put into a drier and dried at a temperature of 70 ° C. for 12 hours.

The dried product was put in a stirrer, and 1.5 kg of glucose was added thereto, followed by stirring together to form a paste solid.

This solid was put into a granulator and granulated to a size of 1 mm in width, 1 mm in length, and 1 mm in height.

The granules were placed in a drying chamber and dried at 45 to 50 ° C. for 2 hours to prepare a herbal feed additive for broilers of the present invention.

Example 2 Preparation of Broiler Forage Added with Herbal Feed Additives

We purchased Domatech's broiler electric feed which is usually fed at broiler time.

To 99 kg broiler feed was added 1 kg of the herbal feed additive of the present invention prepared in Example 1, and then stirred and mixed to prepare a broiler feed containing another herbal feed additive of the present invention.

Experimental Example 1 Antimicrobial Effect Test against Enteric Bacteria of the Herbal Feed Additives of the Present Invention

(1) Preparation of experimental strain

Salmonella Enteritidis ATCC # 13076, S. typhimurium ATCC # 14028, S. pullorum ATCC # 9120, Salmonella Gallinarum ATCC # 9184 , Bacillus cereus ATCC # 11778 (from the National Institute of Veterinary Research), Staphylococcus aureus and Listeria monocytogenes scott A HPB # 3 and Escherichia coli 0157 ( Escherichia coli 0157) Eight strains of H7 (pre-sale in Canada) were prepared.

Each strain was inoculated in 10 ml Tryptic Soy Broth ( Difco, USA ), incubated at 37 ° C. for 18 hours, and then centrifuged at 3,000 g for 10 minutes for 0.1 M phosphate buffer solution (pH). 7.0), after centrifuging twice, the strain was set to the McFarland standard turbidity of 0.5.

(2) Extraction of Antimicrobial Agents from Herbal Feed Additives

The herbal feed additive prepared in Example 1 of the present invention and water were mixed at a ratio of 1:10 and bathed in a water bath at 80 ° C. for 3 hours.

The bath solution was experimented using a solution obtained by filtering with a 0.45 μm filter.

(3) Minimum growth inhibitory concentration measurement

The minimum inhibitory concentration (MIC) of the filtrate for the test strains was determined by the agar dilution method according to the National Committe Clinical Laboratory Standard (NCCLS, 1997).

Diluted the filtrate with phosphate buffer (PBS) twice and adjusted to 10 ⁵ CFU / ml in Mueller Hinton Medium ( Difco, USA ) containing each concentration of multiple inoculator Inoculated.

Next, cultured at 37 ° C. for 18-24 hours, the concentration at which bacterial growth was inhibited in the medium containing the lowest concentration of filtrate was expressed as the minimum growth inhibitory concentration (%).

The minimum growth inhibitory concentration for each bacterium was investigated and the results are shown in Table 1 below.

<Table 1> Minimum Growth Inhibitory Concentration of Herbal Feed Additives for Bacteria

Microorganism types Minimum growth inhibitory concentration (%) 0.2 0.1 0.5 0.25 0.13 Bacillus cerus - - - - + Escherichia coli O157: H7 - + + + + Listeria monocytogenes Scott A - - - - + Salmonella enteritidis - + + + + Salmonella gallimarum - + + + + Salmonella pullorum - + + + + Salmonella typhimurium - + + + + Staphylococcus aureus - - - - +

+; Strain occurs. -; No strain occurred.

As shown in Table 1, Salmonella enteritidis, Salmonella typhimurium, Salmonella pullorum, Salmonella gallinarum, E. coli 0157 (E coli O157: H7) showed an antimicrobial effect at concentrations higher than 2.0%.

Listeria monocytogenes, Staphylococcus aureus And About B. cereus It showed antimicrobial effect at 0.25%.

Experimental Example 2 Effect Test of Broiler Forage Prepared with Herbal Feed Additives

(1) laboratory animals and feed

Experimental animals used 40-week-old inferior broodstock of two-week-old broodstock and 50-week-old broilers purchased from farms.

Salmonella Negative individuals were selected in the separation test, and cages were divided into five groups by experiment and control.

By the method of Example 2, broiler feed prepared by adding the prepared herbal feed additives was fed to the experimental group.

The control group received commercial broiler hen diet.

The place of breeding was the SPF experimental animal breeding laboratory at the Gyeongsang National University Institute of Life Science . Feed and water were fed three times a day.

(2) Salmonella infection and weight measurement experiment

Salmonella enteritidis ATCC # 13076 strain was inoculated in Tripic Soy Broth ( Difco, USA ) and used for 18 hours at 37 ° C. The bacterial solution was diluted 10-fold step with phosphate buffer solution (PBS; pH 7.2), adjusted to 10 ⁷ CFU / ml, and then orally inoculated twice every other day by 0.2 ml in a two-week-old analogy using an oral infusion tube (Sonde). It was.

All experimental animals were examined for diarrhea at 1, 7, and 14 days after Salmonella inoculation, and the average weights of the individual animals were measured before autopsy.

Diarrhea and weight change were investigated for Salmonella infection group and the results are shown in Table 2 below.

<Table 2> Diarrhea and Weight Change in Salmonella Infected Group

Treatment group diarrhea Body weight (g) 1 ^a 7 14 One 7 14 Control 0 ^b   4  0 370.18 ± 12.60 ^c 726.10 ± 23.20 ^c 790.60 ± 78.57 ^A Experimental group   0   One  0 404.30 ± 9.60 ^c 812.65 ± 25.13 ^c 914.42 ± 15.35 ^A

 a; Date after inoculation, b; Positive population from 5 broilers tested

c; Standard error of mean ± broiler number, A; Significant difference (p <0.05).

The experimental group showed diarrhea in 1 of 5 water and 7 of 5 water in control group after 7 days of infection, but after 14 days, all groups did not show diarrhea.

Body weight of the experimental group increased from 404.30 ± 9.60 g at 1 day after inoculation to 914.42 ± 15.35 g at 14 days, which was significantly increased compared to 370.8 ± 12.60 g and 792.60 ± 8.57 g of the control group (p <0.05). ).

<Experiment 3> Among the organs and cecum contents of experimental animals Identification test of Salmonella

(1) Salmonella was orally inoculated to the test animals, and the test group and the control group were bred in the same manner as in (1) of Experimental Example 2.

On the 1st, 7th and 14th day of inoculation, the experimental animals were slaughtered by neck arterial cutting to aseptically collect liver and spleen, and then about 1 g of sample was ground and 10 ml of selenite broth was used. After 18 hours of incubation, 0.1 ml of this was inoculated in MacConkey agar ( Difco, USA ) and Xylose-lysin desoxycholate agar ( Difco, USA ) at 37 ° C. Incubated for 24 hours.

On the other hand, 1 g of cecum content was diluted 10-fold with phosphate buffer solution (PBS) and 0.1 ml of MacConkey agar ( Difco, USA ) and Xyrose-lysine desoxycholate agar medium (XLD agar; Difco) , USA ) and incubated for 24 hours at 37 ℃.

Next, colonies representing typical characteristics of Salmonella were calculated and converted to logs to represent average values.

① Isolation of Salmonella from the Caecum Content

Salmonella from the Caecum Content of Infected Groups The results of the isolates are shown in Table 3.

Table 3 Isolation of Salmonella from the Caecum Contents of Broiler Chickens after Oral Inoculation

Treatment group Date after oral vaccination ^a One 7 14 Control 6.01 ± 0.36 ^B (5/5) ^c 7.27 ± 0.51 ^Aa (5/5) 3.15 ± 1.25 ^Ba (4/5) Experimental group 5.59 ± 0.27 ^A (5/5) 5.67 ± 0.76 ^Ab (5/5) 0 ^Bb (0/5)

a; Each animal was inoculated with Salmonella enteritidis at 4.5 × 10 ⁷ CFU / ml.

b; This is the number of Salmonella enteritidis defined by plate media using MacConkey agar and XR-lysine desoxycholate agar (XLD agar), with an average value of ± 5 broilers. Expressed as a standard error (log ₁₀ ) .

c; Positive number of broilers / inoculated.

A; Significant difference (p <0.05).

The experimental group decreased from log 5.59 ± 0.27 CFU / g at 1 day after inoculation to log 5.67 ± 0.76 CFU / g at 7 days, but was not separated after 14 days. In contrast, the control group, after bacteria inoculation log 6.01 ± 0.36 CFU / g, 7 days while increasing log 7.27 ± 0.51 CFU / g were separated by 14 days, log 3.15 ± 1.25 CFU / g. (P <0.05).

② Salmonella among the organs of infection Separation frequency

Table 4 shows the results of isolates from the liver, spleen and cecum at 7 and 14 days after inoculation.

<Table 4> The isolates from the digestive tract after inoculation

Treatment group Broiler Positive / Number of Test Subjects liver spleen Caecum Control Individual surviving day 7 5/5 5/5 5/5 Individual surviving day 14 2/4 2/4 4/4 Mortality during the experiment 1/10 1/10 1/10 Experimental group Individual surviving day 7 2/5 3/5 5/5 Individual surviving day 14 0/5 0/5 0/5 Mortality during the experiment 0/10 0/10 0/10

In the control group, bacteria were isolated from all 5 numbers of livers and spleens at 7 days, and after 14 days, 2 bacteria in 4 waters, respectively, whereas the experimental group was 2 in 5 waters and 5 in waters at 7 days after infection. It was isolated from 3 water but not from all organs after 14 days.

After 14 days in the cecum, bacteria were isolated from all individuals in the control group, but not in the experimental group.

In the case of the control group, a single cell died around 8 days, and bacteria were isolated from each organ of the carcass.

(2) Scanning Electron Microscopic Observation of Enterobacteriaceae

At necropsy, the caecum was extracted, washed lightly with cold phosphate buffer (PBS), and then dipped in 2.0% glutaraldehyde (Sorensen's, PH7.4) and fixed at 4 ° C.

Next, washed three times at an interval of 1 hour with 0.1 M phosphate buffer solution (PH 7.2), and dried in a critical dryer after dehydration in 70, 80, 90, 95% and absolute ethanol.

The dried tissue piece was transferred to a block using double-sided tape, and surface-treated with pure gold in an ion-plated ion sputtering coater, and observed with a scanning electron microscope (JSM 6400, Scanning Microscope, Oxford).

The microbial settlement of the cecum was observed by scanning electron microscopy (SEM), and as shown in FIG. ) Was observed.

After 14 days, in the control group (C), many bacteria were attached to the intestinal mucosa.

Experimental Example 4 Salmonella from the Feces of Broiler Induction Experiment to investigate the isolation rate of fungi

(1) animal and feed animals

The animals were stocked with 5 days old white broiler chickens, and divided into experimental and control groups, each of 4,000 animals and reared for 4 weeks.

The experimental group was fed the feed prepared by adding the herbal additives of Example 2, the control group was fed the broiler chicken feed.

Feed and water were allowed to feed freely.

Antibiotics were administered to drinking water for 4 days after stocking.

(2) Sampling

Immediately before shipment, a total of 586 feces were collected from 239 feces of the experimental group and 347 feces of the control group.

Fecal samples were collected about 5 g using disposable hygiene gloves (poly glove) and the bacteria were separated within 24 hours.

(3) Isolation and Identification of Salmonella spp.

The isolate of Salmonella spp. Was inoculated with 10 g of selenite cystein broth ( Difco, USA ) by inoculation with 1 g of feces and enriched at 37 ° C. for 24 hours, and then 0.1 ml of the contaminated medium was used as MacConkey agar; Difco, USA ) and gyro-lysine desoxycholate agar medium (XLD agar; Difco, USA ) were inoculated and incubated for 24 hours at 37 ℃. Form a colorless colony in MacConkey agar ( Difco, USA ), and a yellow or orange colony (H) in gyro-lysine desoxycholate agar medium (XLD agar; Difco, USA ) ₂ S positive) or colonies (H ₂ S negative) that did not turn black but were pink or pale yellow in their entirety were purely separated in Neutrient agar. Next, inoculated in Triple Sugar Iron Agar ( Difco, USA ) and incubated at 37 ° C. for 24 hours, the strain showing alkaline slant / acid butt was estimated to be Salmonella spp.

The investigation results are shown in Table 5 below.

TABLE 5 Salmonella from the Feces of Broiler Induction Investigation of the isolation rate of fungi

Treatment group Fecal population Positive reaction water Positive reaction% Control 347 84 24.2 Experimental group 239 25 10.5 Sum 586 109 18.6

Salmonella from the feces of broiler analogy just before shipment As a result of examining the isolation rate of the fungi, as shown in Table 5, 84 isolates of the 347 isolates showed 24.2% isolation rate, and the experimental group was only 25 out of 239 (10.5%).

Experimental Example 5 Mortality and Salmonella Separation Rate

(1) In the experimental group and the control group, the total number of dead individuals was counted for 4 weeks from 5 days after stocking to just before shipping, and was converted into a percentage (%).

The mortality was investigated during 32 days of breeding and the results are shown in Table 6.

<Table 6> Mortality Survey during 32-Day Breeding

Treatment group Number of experimental chicken Dead population % Control 4,000 280 8.0 Experimental group 4,000 202 5.1

As shown in Table 6, the experimental group showed a mortality rate of 5.1% with 202 deaths in 4,000 water, whereas the control group had a high mortality rate of 320 numbers (8.0%) in 4,000 waters.

(2) Feces and organs of infections and pulmonary bodies Salmonella Isolation

The frequency of isolation of Salmonella sp. From the liver, spleen, and caecum contents of dead individuals after 3 weeks of age was investigated.

Salmonella spp. Was isolated from the liver, spleen and caecum contents of the deceased individuals during the experimental period. The control group was isolated from 1 out of 10 livers and spleens and 3 cecals, but in the experimental group, 1 out of 7 waters were isolated. (Table 7).

<Table 7> Salmonella spp. Isolated from liver, spleen and intestinal contents of dead chicken during experimental period

Treatment group Number of chickens tested Positive population liver spleen Caecum Control 10 One One 3 Experimental group 7 0 0 0

(3) Effect of feed additives on sensory

In order to investigate the effects of feed additives on the sensory performance of broilers, the effects of feed additives were mixed homogeneously (1%) on feed and then on the sensory effects on 32 days of feeding. The results are shown in Table 8.

<Table 8> Effect of Feed Additives on Sensory Performance

Item Control Experimental group Color 7.56 7.44 smell 7.67 7.56 Succulent Sex * 6.00 7.56 * year* 6.22 7.89 * Comprehensive Preference * 6.33 7.78 *

 * The items of sensory test are conducted according to the sensory test item of the Korea Food Research Institute.

 9: Very very high (or good) 1: Very very low (or not)

The experimental group was 7.56 higher than the control group's succulent 6.0, and the year was 7.56 higher than the control group's 6.0, and the overall acceptability was 7.78 higher than the control group's 6.33.

Experimental Example 6 Effect of Herbal Feed Additives on Broiler Specifications

In order to investigate the effect of feed additives on broiler specifications, the effect of feed additives on the feed was homogenously mixed to 1%, and the effects on feed specifications were examined at 32 days and the results are shown in Table 9.

<Table 9> Effects of Herbal Feed Additives on Broiler Specifications

Contents Experimental group Control result Remarks Average weight 1.73 kg 1.69 kg + 0.04kg Growth rate 96% 92% + 4% -4% Mortality 5% 8% -3% -3% Feed requirement 1.73% 1.82% -0.09%

The average weight of the control group was 1.69 kg, which was higher as the experimental group was 1.73 kg, and the growth rate was 96% higher in the experimental group than the control group 92%.

 Mortality was 5% lower in the experimental group compared to 8% of the control group, and feed demand was 1.73% in the experimental group compared to 1.82% in the control group.

The herbal feed additive for broiler chickens of the present invention inhibits enterobacteria such as Salmonella, weight gain, high growth rate, reduced disease incidence, low mortality rate, low feed requirement, and herbal preparation additives Is provided.

In addition, there is provided a high-quality broiler feed having high safety with the addition of the herbal feed additive of the present invention.

Figure 1 is a scanning microscope observation picture of the intestinal settle bacteria.

A: Bacillus on the epithelial surface of the intestinal mucosa of the control group 7 days after infection

B: Inflammatory response of the mesenteric epithelial tissue of control group 7 days after bacterial infection

C: Intestinal mucosa of control group 14 days after bacterial infection

D: Intestinal membrane of experimental group 14 days after fungal infection

Claims (4)

Prepare 10% by weight of gold, 15% by weight of Seokchangpo, 10% by weight of saengchocho, 30% by weight of safflower, 10% by weight of goji berry, 10% by weight of anti-air, and 15% by weight of glucose, blending and pulverizing the remaining herbs except glucose After drying, glucose is added to the dried material, the mixture is stirred together to form a solid in paste state, the solid is granulated and dried to produce a herbal feed additive. The method of claim 1, wherein the herbal medicines are blended, ground and dried for 12 hours at a temperature of 70 DEG C when drying. Herbal feed additive prepared by the method of claim 1 to claim 2. Broiler feed prepared by adding 0.8 ~ 1.2% by weight of the herbal feed additive of claim 3.