KR100492916B1 - Lactobacillus for improving weight gain and environmental condition in brolier - Google Patents

Abstract

The present invention relates to lactic acid bacteria and feeding method effective for improving broiler breeding and improving the breeding environment, and to provide lactobacillus Lactobacillus reuteri avibro2 isolated from the caecum of 5 week old broilers.

Since the lactic acid bacteria of the present invention are derived from a poultry digestive organ, it has been shown to be excellent in acid resistance and bile resistance, thereby making it easy to survive in the digestive organ.

In particular, when the feed is added to the feed of broiler chickens of the same breed, feed intake is increased, weight gain and feed rate is improved to improve the productivity.

In addition, the quality of the chicken is improved by reducing the moisture content of the bottom litter by inhibiting the occurrence of stool, it is possible to suppress the exhaustive diseases such as respiratory diseases by reducing the amount of ammonia in the system.

Description

LACTOBACILLUS FOR IMPROVING WEIGHT GAIN AND ENVIRONMENTAL CONDITION IN BROLIER}

The present invention relates to lactic acid bacteria, and more particularly, to provide a novel lactic acid bacteria that can contribute to the enhancement of broilers when fed to broiler feed by maintaining the growth and normal metabolism of harmful bacteria by producing a lactic acid, and feed It is about a method.

Generally, in the case of poultry, the microorganisms are present in the digestive tract while ingesting the environment and feed after incubation. In broilers around 7-10 days, a certain microorganism similar to the stellar system is established, and each microorganism plays a role in symbiosis or competitive exclusion. Microorganisms useful for host animals are involved in preventing colonization of pathogenic organisms, enhancing immune response development, producing vitamins, and digesting nutrients.

On the other hand, chickens are subject to a variety of stress during the breeding process, the stress factors are movement, harmful gas, poor feed, noise, overcrowding, vaccination, cold, heat and the like. This stress causes intestinal microbes to lose balance and weakens the body's defenses. For this reason, broiler feed currently uses antibiotics.

However, the use of antibiotics in livestock raises the problem of residues in the production, and the human body causes problems in the production of drug-resistant organisms, and in developed countries such as Europe and the United States, it is possible to produce hygienic livestock products by using probiotics for antibiotic replacement. Research is underway to increase the preference of consumers and consumers.

Probotics are probiotics and proliferation promoters that are defined as having a beneficial effect on bacterial flora inherent to plants or food as well as animals, and include bacteria (Bacteria), fungi, and yeasts. Lactobacillus is one of the most widely used probiotics are Bifidobacterium , Streptococcus , Lactobacillus . Lactobacillus is a bacterium that exists throughout the digestive tract, producing lactic acid to inhibit the growth of harmful bacteria and maintain normal metabolic activity. Sometimes.

On the other hand, in order for probiotics to survive in the digestive tract of livestock, it must basically have low pH of stomach and resistance to bile of duodenum. It should also not be affected by survival by the feed.

Origin of lactic acid bacteria used as a probiotic in livestock originated from soil and animals However, at present, most of them are separated from animals such as cows and pigs, and lactic acid bacteria derived from poultry digestive organs have not yet been reported, and poultry lactic acid bacteria have not been produced in Korea.

However, because microorganisms derived from the same livestock survived under similar conditions, it is easier to survive than other strains when fed to the same livestock, and birds of chickens have a slightly different digestive organs than mammals such as ruminants or pigs. This raises the need for probiotics for poultry.

Accordingly, the present invention provides a poultry lactic acid bacterium derived from a poultry digestive organ and a feed feeding method, and when added to a feed of broilers of the same breeding animal, the purpose of the present invention is to increase feed intake and weight gain compared to other strains. have.

The object can be achieved through Lactobacillus reuteri avibro2 isolated from the caecum of a 5 week old broiler.

Hereinafter, the present invention will be described in more detail with reference to the accompanying drawings.

In the present invention, 11 strains of lactic acid bacteria were isolated from the cecum recognized to be the best among the lactic acid bacteria in the digestive tract, and two strains having excellent acid resistance and bile resistance were identified.

Lactobacillus reuteri avibro 2 (Accession No. KFCC-11196) was isolated and identified as Lactic acid bacterium Lactobacillus ruteri abibrotu.

end. Morphology

ㆍ Rpgoas agar medium at 37 ° C for 2 days

 Cell type: bacilli

 Mobility: None

 -Spore Formability: None

 Gram staining: positive

I. Physiological properties

1) Optimum growth temperature: 37 ～ 39 ℃

2) Optimum growth pH: 6.3 ～ 6.7

3) Effects on Oxygen: Breathable Anaerobic

4) Lactic acid production status: +

All. Identification result of strain (16S rRNA sequencing)

① Pyrogenic tree

 See Figure 1

In the above results, avibor2 was found to be closest to the Lactobacillus rutheri.

The characteristics test results for the above lactic acid bacteria will be described in detail below.

Example 1 Acid Resistance and Bile Resistance Results

Experimental method) In the acid resistance investigation, each of the 11 strains of cecum-derived lactic acid-producing bacteria was incubated in MRS broth at 37 ° C for 24 hours, and centrifuged at 2000g to sterilize pellets at the bottom. Wash twice with PBS. 0.1 ml was inoculated in 2 ml of PBS adjusted to pH 1, 2, 3, 4, and growth was observed at 37 ° C. for 4 hours. Bile resistance was inoculated with 10 ml of MRS broth added 0.3%, 0.5% Bile salt in culture culture in MRS broth for 24 hours at 37 ℃. The inoculated culture was stored at 37 ° C. for 6 hours and absorbance was measured with a colorimeter at 660 nm every hour.

Results) [Table 1] shows the acid resistance results, and compared the development strains among the 11 weeks of the cecal-derived lactic acid bacteria and one of the 9 unidentified lactic acid bacteria. The unidentified lactic acid bacteria showed similar results to the unidentified strains of [Table 1].

TABLE 1

Comparison of Acid Resistance of Lactic Acid Bacteria and Developmental Strains in Broiler Caecum

Strain pH Incubation time (log 10 cfu) 0 h 0.5h 1h 2h 3h 4h Midongeng Lactobacillus Lactobacillus One 8.119 - - - - - 2 9.141 - - - - - 3 8.998 8.677 7.707 6.536 4 8.864 6.619 8.761 8.259 8.360 8.867 Lactobacillus reuteri avibro 2 One 6.631 - - - - - 2 8.722 5.640 4.093 - - - 3 7.787 7.734 8.619 8.748 7.741 7.540 4 7.806 8.382 8.357 8.593 8.075 8.259

In the above results, all of the lactic acid bacteria at pH 1 and 2, most of the lactic acid bacteria were killed immediately upon incubation, but Lactobacillus reuteri avibro 2 was found to withstand up to 1 hour. At pH 3, Lactobacillus reuteri avibro 2 was shown to be viable and the remaining strains were killed within 1 to 2 hours after incubation. At pH 4, most strains were found to be viable.

In Table 2 below, the bile resistance of the developed and general strains is compared.

As a result of biliary resistance, the developmental strain Lactobacillus reuteri avibro 2 was found to be more resistant to bile than normal lactic acid bacteria.

TABLE 2

Comparison of Bile Resistance between Lactic Acid Bacteria and Developmental Strains in Broiler Caecum

Strain Bilesalts (%) Absorbance (660nm) 0h 1h 2h 3h 4h 5h 6h Midongeng Lactobacillus Lactobacillus 0 0.038 0.057 0.179 0.568 1.010 1.224 1.429 0.3 0.042 0.049 0.035 0.018 0.009 0.018 0.001 0.5 0.042 0.016 0.017 0.001 0.002 0.003 0.003 Lactobacillus reuteri avibro 2 0 0.058 0.082 0.064 0.309 0.916 1.681 1.791 0.3 0.076 0.081 0.115 0.165 0.214 0.231 0.292 0.5 0.073 0.076 0.112 0.163 0.250 0.285 0.341

In the above experimental results, the lowest pH of broiler digestive organs is located in the stomach, and in general, since the pH is about 4-5 when feed is ingested, most of the lactobacillus derived from caecum is added to the feed and survived even if it passes through the stomach. However, the resistance to bile showed a severe difference between strains. Bile is encountered when feeding plants reach the stomach and reach the duodenum, which has the greatest impact on the survival of lactic acid bacteria. This is because lactic acid bacteria have higher adaptability to acid because they are lactic acid bacteria. The developed strains proved to have probiotic value because they had better resistance to bile than most common strains.

<Example 2. Broiler Specification Test>

Experimental Method) The five weeks feeding trial was conducted in the stereographic by the addition of lactic acid bacteria in the development 10 ⁴ ~10 ⁷ cfu per g level in broiler feed. Comparative Example is a lactobacillus-free addition, Experimental Example is the addition of Lactobacillus reuteri avibro 2 to broiler feed at this level. The experimental feeds contained 3,106 kcal / kg of metabolic energy and 22.02% of crude protein up to 1 to 3 weeks of age, and 3,102 kcal / kg and 20.01% of 3 to 5 weeks of age, respectively. The moisture content of the litter and the amount of ammonia in the litter were measured to investigate the environmental conditions in the house. The bottom litter was sampled at 1, 3 and 5 weeks of age immediately before the specification test and during the breeding process. The ammonia in the powder was collected from fresh ginseng and stored in a gas collection container at room temperature under aerobic conditions. Measured daily.

Results) [Table 3] shows the change in weight by age, the experimental example from 1 week of age was faster growth rate than the comparative example, the weight per 1 at 5 weeks of the experimental example was 85g higher than the comparative example.

TABLE 3

Comparison of weight change between the comparative example and the experimental example of the present invention (unit: g)

1 day old 1 week old 2 weeks old 3 weeks old 4 weeks old 5 weeks old Comparative example 51.3 128.7 331.5 687.8 1,145 1,665 Experimental Example 51.2 132.4 353.1 726.2 1,221 1,750

[Table 4] shows the feed efficiency, but the amount of feed intake was increased by about 54g compared to the comparative example, and the feed rate was significantly improved.

TABLE 4

Feed Efficiency Comparison between Comparative Example and Experimental Example of the Invention

Allowance Feed Intake (g) Feed rate Comparative example 2,803 1.739 Experimental Example 2,857 1.682

[Table 5] is a comparison of the moisture content of the bottom litter, the experimental period was found to decrease the moisture content as compared to the comparative example as the breeding period is over three weeks.

TABLE 5

Comparison of moisture content in litter between Comparative Example and Experimental Example of the present invention (unit%)

Chuseok 1 week old 3 weeks old 5 weeks old Comparative example 12.46 12.55 23.40 38.3 Experimental Example 12.46 12.80 24.88 28.5

[Table 6] is a comparison of the amount of ammonia gas generated by the system powder, and when the amount is generated up to 4 days, the experimental example is 2 to 4.6 times lower than the comparative example.

TABLE 6

Comparison of Ammonia Generation Amount in Comparative Example and Experimental Example of the Invention (Unit: ppm)

0 One 2 3 4 5 6 7 Comparative example 0 5.7 38.7 470 1233 587 513 913 Experimental Example 0 0.8 7.8 335 267 793 813 680

The increase in feed intake in the specification test of the development strain increased the palatability and digestive function of the feed due to the development strain, thus improving productivity such as weight gain and feed efficiency. In addition, broiler chickens are generally raised in plain sand, so if the water content of the bottom litter is high, chicken breast quality is often lowered due to increased thoracic edema, and hydrophilic ammonia is not released, causing mortality and increased consumption. do. The developmental strain reduces the incidence of stool, such as E. coli diarrhea due to the increase of gut beneficial bacteria. The moisture content of the bottom litter was reduced, and the ammonia gas generation was greatly reduced compared to the comparative example due to the improvement of nitrogen compound digestibility and the increase of microorganisms in the powder.

As described above, the lactic acid bacteria of the present invention are derived from a poultry digestive organ, and thus have excellent acid resistance and bile resistance, and thus, survival in the digestive organs is easy.

In particular, when the feed is added to the feed of broiler chickens of the same breed, feed intake is increased, weight gain and feed rate is improved to improve the productivity.

In addition, the quality of the chicken is improved by reducing the moisture content of the bottom litter by inhibiting the generation of stool, and by reducing the amount of ammonia in the system, it will have the effect of suppressing consumable diseases such as respiratory diseases.

1 is a schematic diagram of a Pyrogenic tree of the present invention lactic acid bacteria (Lactobacillus ruteri abibrotu).

Claims (2)

Lactobacillus reuteri avibro2 (Accession No. KFCC-11196), a lactic acid bacterium derived from the digestive system of broiler chickens, excellent in acid resistance and bile resistance, and excellent in improving weight gain and reducing ammonia gas generation in poultry. 10 ^4-10 how to pay the feed mixed with ⁷ levels cfu per g feed the strain of claim 1.