KR100464225B1 - Method for identification and analysis of specific molecules using single strand nucleic acid ligands - Google Patents

Method for identification and analysis of specific molecules using single strand nucleic acid ligands Download PDF

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Publication number
KR100464225B1
KR100464225B1 KR1020040008760A KR20040008760A KR100464225B1 KR 100464225 B1 KR100464225 B1 KR 100464225B1 KR 1020040008760 A KR1020040008760 A KR 1020040008760A KR 20040008760 A KR20040008760 A KR 20040008760A KR 100464225 B1 KR100464225 B1 KR 100464225B1
Authority
KR
South Korea
Prior art keywords
nucleic acid
single stranded
stranded nucleic
analysis
identification
Prior art date
Application number
KR1020040008760A
Other languages
Korean (ko)
Inventor
김성천
Original Assignee
제노프라 주식회사
김성천
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by 제노프라 주식회사, 김성천 filed Critical 제노프라 주식회사
Priority to KR1020040008760A priority Critical patent/KR100464225B1/en
Application granted granted Critical
Publication of KR100464225B1 publication Critical patent/KR100464225B1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C01—INORGANIC CHEMISTRY
    • C01B—NON-METALLIC ELEMENTS; COMPOUNDS THEREOF; METALLOIDS OR COMPOUNDS THEREOF NOT COVERED BY SUBCLASS C01C
    • C01B13/00—Oxygen; Ozone; Oxides or hydroxides in general
    • C01B13/02—Preparation of oxygen
    • C01B13/0229—Purification or separation processes
    • C01B13/0248—Physical processing only
    • C01B13/0259—Physical processing only by adsorption on solids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
    • B01D53/02—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols by adsorption, e.g. preparative gas chromatography
    • B01D53/04—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols by adsorption, e.g. preparative gas chromatography with stationary adsorbents
    • B01D53/0407—Constructional details of adsorbing systems
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2256/00—Main component in the product gas stream after treatment
    • B01D2256/12—Oxygen
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2257/00—Components to be removed
    • B01D2257/10—Single element gases other than halogens
    • B01D2257/102—Nitrogen
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2259/00—Type of treatment
    • B01D2259/40—Further details for adsorption processes and devices

Abstract

PURPOSE: Provided is a method for identifying and analyzing a specific substance by using single stranded-nucleic acid ligand which is capable of forming a thermodynamically stable complex. It is used for detection and analysis of biomolecules including microorganisms, cells and proteins. Therefore, it is applied to medicine, veterinary medicine, food technology and agriculture. CONSTITUTION: The method for identifying and analyzing a specific substance using single stranded-nucleic acid ligand is characterized by the steps of: preparing a molecular beacon comprising a single stranded nucleic acid ligand having 15-100bp of a nucleotide sequence, single stranded nucleic acid of which 5' end is bound with fluorophore, and a single stranded nucleic acid of which 3' end is bound with quencher; forming a complex by reacting the single stranded nucleic acid ligand with a sample containing complementary single-stranded nucleic acid or a specific substance; and detecting the complex by fluorescent signals.
KR1020040008760A 2004-02-10 2004-02-10 Method for identification and analysis of specific molecules using single strand nucleic acid ligands KR100464225B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020040008760A KR100464225B1 (en) 2004-02-10 2004-02-10 Method for identification and analysis of specific molecules using single strand nucleic acid ligands

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020040008760A KR100464225B1 (en) 2004-02-10 2004-02-10 Method for identification and analysis of specific molecules using single strand nucleic acid ligands

Publications (1)

Publication Number Publication Date
KR100464225B1 true KR100464225B1 (en) 2005-01-03

Family

ID=37383511

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020040008760A KR100464225B1 (en) 2004-02-10 2004-02-10 Method for identification and analysis of specific molecules using single strand nucleic acid ligands

Country Status (1)

Country Link
KR (1) KR100464225B1 (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20140118680A (en) * 2013-03-27 2014-10-08 김성천 Nucleic acid chip and Reference Substance for Obtaining Bind information of sigle-stranded nucleic acid and Biomolecule, Manufacturing Method Thereof, and Method and apparatus for analyzing Biomolecules Using Nucleic acid chip and Reference Substances
WO2015060511A1 (en) 2013-10-22 2015-04-30 주식회사 바이오이즈 Marker for generating binding information on biomolecules and nucleic acids, preparation method therefor, and method and apparatus for analyzing biomolecule by using same
WO2015060609A2 (en) 2013-10-21 2015-04-30 주식회사 바이오이즈 Method and apparatus for analyzing biomolecules by using oligonucleotide
KR101783657B1 (en) 2013-11-25 2017-10-10 주식회사 바이오이즈 Apparatus, Kits and Methods for Analyzing Biomolecuels
WO2018084594A1 (en) 2016-11-02 2018-05-11 김성천 Quantitative cluster analysis method of target protein by using next-generation sequencing and use thereof

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20140118680A (en) * 2013-03-27 2014-10-08 김성천 Nucleic acid chip and Reference Substance for Obtaining Bind information of sigle-stranded nucleic acid and Biomolecule, Manufacturing Method Thereof, and Method and apparatus for analyzing Biomolecules Using Nucleic acid chip and Reference Substances
WO2015060609A2 (en) 2013-10-21 2015-04-30 주식회사 바이오이즈 Method and apparatus for analyzing biomolecules by using oligonucleotide
WO2015060511A1 (en) 2013-10-22 2015-04-30 주식회사 바이오이즈 Marker for generating binding information on biomolecules and nucleic acids, preparation method therefor, and method and apparatus for analyzing biomolecule by using same
KR101783657B1 (en) 2013-11-25 2017-10-10 주식회사 바이오이즈 Apparatus, Kits and Methods for Analyzing Biomolecuels
WO2018084594A1 (en) 2016-11-02 2018-05-11 김성천 Quantitative cluster analysis method of target protein by using next-generation sequencing and use thereof

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