KR100462257B1 - Method of cultivation for effective micro-organisms - Google Patents

Method of cultivation for effective micro-organisms Download PDF

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KR100462257B1
KR100462257B1 KR10-2002-0088565A KR20020088565A KR100462257B1 KR 100462257 B1 KR100462257 B1 KR 100462257B1 KR 20020088565 A KR20020088565 A KR 20020088565A KR 100462257 B1 KR100462257 B1 KR 100462257B1
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minerals
stone
filled
culture medium
microorganisms
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KR20040062221A (en
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서희동
서영진
서영준
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서희동
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2529/00—Culture process characterised by the use of electromagnetic stimulation

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Abstract

본 발명은 농업분야, 환경분야, 식품분야, 양어장, 가축의 음용수처리 등의 분야에 이용 할 수 있는 유용미생물의 배양방법을 제시하는 것이 목적이다.An object of the present invention is to provide a method for culturing useful microorganisms that can be used in fields such as agriculture, environment, food, fish farming, and drinking water treatment of livestock.

이를 위하여 본 발명은, 미생물배양조(1)에 부식물질 펠렛트(Pellet)와 미네랄(Minerals)용출이 용이한 광물 및 원적외선이나 음이온을 방사하는 광물(3) 등을 충전(充墳)한 생물반응기(Bio-reactor ; 2)를 설치하고, 배양액과 종균을 주입하고 미생물배양조(1)와 생물반응기(2)에 공기를 공급하여 폭기를 하면 배양액은 생물반응기(2)의 부식물질 펠렛트와 광물(3)의 충전층을 순환하면서 미생물생육에 필요한 미네랄 및 활성효소성분 등을 공급받아 활성화된 미생물을 배양하며, 이때 보다 활성화된 미생물을 배양하기 위해서 미생물배양조(1)의 배양액을 자속밀도(磁束密度)가 10,000가우스(Gauss)이상 착자된 영구자석이나 전자석(5)의 자계(磁界) 및 자화된 자철광(8)이 충전된 자화탱크(7)을 순환시킨다.To this end, the present invention is a microbial culture tank (1) filled with a corrosive pellet (Pellet) and minerals (Minerals) easy to dissolve minerals and minerals (3) that emits far infrared rays or anions (등) When a reactor (Bio-reactor; 2) is installed, the culture medium and the seed are injected, air is supplied to the microbial culture tank (1) and the bioreactor (2), and the aeration is carried out. The culture medium is a pellet of the corrosive material of the bioreactor (2). And circulating the packed bed of minerals (3) to receive the minerals and active enzyme components necessary for the growth of microorganisms and to cultivate the activated microorganisms, in which case the culture medium of the microorganism culture tank (1) in order to cultivate the activated microorganisms The magnetization tank 7 filled with the magnetic field and the magnetized magnetite 8 of the permanent magnet or electromagnet 5 magnetized with a density of 10,000 Gauss or more is circulated.

그리고, 배양규모가 큰 경우에는 도2에서와 같이 배양액을 저장하였다가 사용하는 경우에는 배양액 저장조(9)의 미생물의 활성도를 유지시키기 위해서는 광물(3)이 충전된 미네랄 용출이 용이한 광물 및 원적외선이나 음이온을 방사하는 광물(11)이 충전된 미네랄반응기(10)를 설치하여 공기를 주입하여 폭기를 한다.In the case where the culture size is large, the culture solution is stored as shown in FIG. 2, and when the culture solution is used, in order to maintain the activity of the microorganisms in the culture medium storage tank 9, minerals and minerals easily filled with minerals 3 are easily released. In addition, a mineral reactor 10 filled with minerals 11 emitting negative ions is installed to inject air to aeration.

Description

미생물배양방법{Method of cultivation for effective micro-organisms}Method of cultivation for effective micro-organisms

본 발명은 부식물질 펠렛트(Pellet)와 미네랄 용출이 용이한 광물 및 원적외선이나 음이온을 방사하는 광물과 자석 및 자철광() 등을 이용하여 농업분야, 환경분야, 식품분야, 양어장, 가축의 음용수처리 등에 이용 할 수 있는 유용 미생물의 배양방법을 제시하는 것이 목적이다.The present invention is a pellet (Pellet) and minerals that are easy to dissolve minerals and minerals and magnets and magnetite that emits far infrared rays or anions ( The purpose of the present invention is to propose a method of cultivating useful microorganisms that can be used for agriculture, environment, food, fish farming, drinking water treatment of livestock.

일본 특허공보 평(平)5-66199호에서는 유기성물질을 함유한 폐수처리에서 일반활성오니처리공정에서 부식토를 펠렛트(Pellet)형태로 가공한 것과 미네랄 용출이 용이한 유문암(流紋岩) 또는 대사이드(Dacite)질의 부석(浮石)을 충전한 생물반응기가 내장된 배양조를 설치하여 침전조에서 폭기조로 반송하는 종오니의 일부를 배양조로 보내어 활성화된 균주를 배양하여 폭기조로 보내어 처리한 결과 일반활성오니처리공정에 비해서 처리효율이 상당히 향상되는 효과가 있었으나 전술한 부식토 및 부석 중에서 자철광함량이 낮거나 자화도가 낮은 경우에는 처리효율이 크게 향상되지 않은 문제점이 있었다.Japanese Patent Laid-Open No. 5-66199 discloses the processing of humus soil into pellets in the general activated sludge treatment process in wastewater treatment containing organic substances, and in the case of rhyolite rock which is easy to dissolve minerals or As a result of installing a culture tank with a bioreactor filled with a crust (Dacite), a part of the sludge returned from the sedimentation tank to the aeration tank was sent to the culture tank, and the activated strain was cultured and sent to the aeration tank. Compared to the activated sludge treatment process, the treatment efficiency was significantly improved. However, when the magnetite content was low or the degree of magnetization was low among the humus and pumice, the treatment efficiency was not significantly improved.

본 발명은 상기와 같은 문제점을 해소하기 위해 농업분야, 환경분야, 식품분야, 양어장, 가축의 음용수처리 등에 이용 할 수 있는 유용미생물의 배양방법을 제공하는 데 본 발명의 목적이 있는 것이다.An object of the present invention is to provide a method for cultivating useful microorganisms that can be used in agriculture, environmental, food, fish farms, drinking water treatment of livestock in order to solve the above problems.

이와 같은 목적을 달성하기 위한 본 발명은, 자화된 자철광, 자석, 부식물질, 미네랄 용출이 용이한 광물, 원적외선이나 음이온을 방사하는 광물 등을 이용하여 활성화된 유용미생물을 배양하는 방법을 제시한다.The present invention for achieving the above object, a method for culturing the activated useful microorganisms using magnetized magnetite, magnets, corrosive substances, minerals that are easy to dissolve minerals, minerals that emit far infrared rays or anions.

도 1은 제1 미생물배양공정도1 is a first microbial culture process

도 2는 제2 미생물배양공정도Figure 2 is a second microbial culture process

〈도면의 주요부분에 대한 부호의 설명〉<Explanation of symbols for main parts of drawing>

1 : 미생물배양조 2 : 생물반응기(Bio-reactor)1: Microbial culture tank 2: Bio-reactor

3 : 부식물질 펠렛트(Pellet) 및 광물 4 : 순환펌프(Pump)3: Corrosive Pellet and Mineral 4: Circulation Pump

5 : 자석 6 : 정류기5: magnet 6: rectifier

7 : 자화탱크(Tank) 8 : 자철광7: magnetization tank 8: magnetite

9 : 배양액 저장조 10 : 미네랄공급기9: culture medium storage tank 10: mineral feeder

11 : 광물11: mineral

이하 도면을 중심으로 본 발명의 내용을 상세히 설명하면 다음과 같다.Hereinafter, the contents of the present invention will be described in detail with reference to the drawings.

생물학적 악취처리의 경우에는 흡수탑의 순환용액을 배양액으로 사용하며, 양어장의 수처리 경우에는 양어장내의 물을 배양액으로 사용하고, 하 ·폐수의 생물학처리의 경우에는 침전조에 침전된 오니를 폭기조로 반송하는 종오니의 일부를배양액 및 종균으로 사용하여 풀브산(Fulvic acid) 함량이 많은 부식물질을 펠렛트(Pellet) 형태로 가공한 것과 미네랄(Minerals) 용출이 용이한 감람석(橄欖石), 각섬석(角閃石), 흑운모(黑雲母), 진주석(珠石), 흑요석(黑曜石), 송지석(松脂石), 유문암(流紋石)이나 대사이드(Dacite)질의 부석(浮石)과 같은 광물을 1종류이상 충전(充墳)하고, 원적외선(遠赤外線)이나 음이온을 방사하는 천매석(千枚石), 맥반석(飯石), 전기석(電氣石), 거정석(巨晶石), 의왕석(王石), 석영섬록암(石英閃綠岩), 귀보석(貴石), 태징석(泰石), 신명석(神明石)과 같은 광물도 1종류이상 충전한 생물반응기(Bio-reactor ; 2)가 내장된 미생물반응조(1)에 공급하면서 송풍기로부터 공기를 공급하여 폭기를 하면 배양액은 생물반응기(2)에 충전된 부식물질 펠렛트 및 광물(3)의 층을 순환하면서 킬레이트(Chelate)성 착염상태의 미네랄(Minerals) 성분과 활성효소 등을 공급받아 미생물은 활발한 대사활동을 하는 상태로 배양된다.In the case of biological odor treatment, the circulating solution of the absorption tower is used as a culture solution.In the case of water treatment of fish farms, water in the fish farm is used as a culture solution.In the case of biological treatment of sewage and wastewater, sludge deposited in the sedimentation tank is returned to the aeration tank. Part of the sludge was used as a culture medium and spawn to process corrosive materials with a high content of Fulvic acid in the form of pellets, and olivine and hornblende, which are easy to dissolve minerals. Stone, biotite, mother-of-pearl One or more kinds of minerals, such as stone, obsidian, songji stone, rhyolite, and diaphragm, are filled with one or more kinds of minerals. Aphrodisiac, anemone stone, and elvan Stone, tourmaline, giant stone, Uiwang stone 王石, Quartz diorite, precious stones 石, Tajing Stone If aeration is supplied by supplying air from the blower while supplying a microbial reactor (1) with a built-in bioreactor (2) filled with one or more kinds of minerals such as stone and shinmyeongseok, 2) Microorganisms are cultured with active metabolic activity by supplying chelating complex minerals and active enzymes while circulating the corrosive pellets and layers of minerals (3). .

이때 보다 활성화된 미생물을 배양하기 위해서는 순환펌프(Pump ; 4)의 토출측에 자속밀도(磁束密度)가 10,000가우스(Gauss)이상 착자된 영구자석이나 전자석(5)을 설치하고, 자화탱크(Tank ; 7)에는 자속밀도가 200가우스이상 착자된 자철광(8)을 충전한 자계(磁界)를 순환하여 자화처리를 한다.At this time, in order to culture more activated microorganisms, a permanent magnet or an electromagnet 5 magnetized with a magnetic flux density of 10,000 Gauss or more on the discharge side of a circulating pump 4 is installed, and a magnetization tank (Tank; 7) magnetizes by circulating a magnetic field filled with magnetite 8 magnetized with a magnetic flux density of 200 gauss or more.

물(유체)이 자석(5)과 자성을 띈 자철광(8)의 자계를 통과하게 되면 자기유체역학(MHD ; Magneto-Hydro-Dynamics)전압이 발생하면서 전자의 파동(波動)에 의해서 물분자의 집단체(集團; Cluster)가 미세화(微細化)되면서 표면장력(表面張力)이 적어져 침투력(浸透力)이 향상되어 용해력이 향상되면서 탈취효율도 향상되고, 미생물의 세포막 투과율이 향상되어 미생물의 대사활동을 활발하게 한다.When water (fluid) passes through the magnetic field of the magnet 5 and the magnetized magnetite 8, the magneto-hydro-dynamics (MHD) voltage is generated and the wave of the electrons Collective ; As the cluster becomes finer, the surface tension decreases, the penetration force is improved, solubility is improved, deodorization efficiency is improved, and cell membrane permeability of microorganism is improved, so that metabolic activity of the microorganism is increased actively. do.

그리고, 필요에 따라서는 미생물생육의 최적의 조건으로 pH 및 산화환원전위(ORP ; Oxidation Reduction Potential)값을 조정한다.And, if necessary, the pH and Oxidation Reduction Potential (ORP) values are adjusted to optimal conditions for microbial growth.

가축의 음용수에 유용미생물배양액을 혼합하여 급여하는 경우나 식품공장에서 유용미생물배양액을 사용하는 경우에는 바칠루스 마이코이데스(Bacillus mycoides), 바칠루스 서브틸리스(Bacillus subtillis), 바칠루스 나토(Bacillus natto), 아스페르길루스 니거(Aspergillus niger), 페니실리움 글로쿰(Penicillum glaucum), 소랑세루로섬(Sorangium cellulosum), 악티노마이세스 그로비오 포러스 로세어스(Actinomyces globioporus roseus), 슈도모나스속(Pseudomonas sp.), 사상균, 방선균, 유산발효균과 같은 필요한 유용미생물의 종균을 초기에는 접종하고, 배양액은 당밀, 한천, 수용성전분, 육즙과 같은 유기물에 키토산(Chitosan)을 첨가한 배양액을 사용하고, pH의 조정은 목초산, 구연산, 주석산과 같은 각종 미네랄성분과 착염을 생성할 수 있는 유기산(有機酸)을 사용하여 부식물질을 펠렛트와 전술한 광물(3)을 충전한 생물반응기(2)가 내장된 미생물반응조(1)에 공급하면서 송풍기로부터 공기를 공급하여 폭기를 하면 배양액은 생물반응기(2)에 충전된 부식물질 펠렛트 및 광물(3)의 층을 순환하면서 킬레이트성 착염상태의 미네랄성분과 활성효소 등을 공급받아 미생물은 활발한 대사활동을 하는 상태로 배양되며, 이때도 보다 활성화된 미생물을 배양하기 위해서는 순환펌프(4)의 토출측에 자속밀도가 10,000가우스이상 착자된 영구자석이나 전자석(5)을 설치하고, 자화탱크(7)에는 자속밀도가 200가우스이상 착자된 자철광(8)을 충전한 자계를 순환하여 자화처리를 한다.Bacillus mycoides, Bacillus subtillis, Bacillus natto, when a mixed microbial medium is fed to drinking water of livestock, or when a useful microbial medium is used in a food factory. natto, Aspergillus niger, Penicillum glaucum, Sour Initially inoculate the seed of necessary useful microorganisms, such as Sorangium cellulosum, Actinomyces globioporus roseus, Pseudomonas sp. The culture solution is a culture solution in which chitosan is added to organic matters such as molasses, agar, water-soluble starch and gravy, and the pH is adjusted to organic acids that can form complex salts with various mineral components such as acetic acid, citric acid and tartaric acid. Iv) by supplying air from the blower while supplying the corrosive material to the microbial reactor (1) in which the bioreactor (2) filled with the pellet and the mineral (3) described above is aerated. 2) Microorganisms are active in metabolic activity by supplying chelating complex minerals and active enzymes while circulating the corrosive pellets and the layers of minerals (3). In order to cultivate more activated microorganisms, a permanent magnet or an electromagnet (5) magnetized with a magnetic flux density of 10,000 gauss or more is installed on the discharge side of the circulation pump (4), and the magnetic flux density is provided in the magnetization tank (7). The magnetization process is performed by circulating a magnetic field filled with magnetite 8 magnetized at least 200 gauss.

그리고, 배양미생물을 저장하였다가 사용하는 경우에는 도2에서와 같이 배양액 저장조(9)를 설치하고, 배양액 저장조(9)에서 미생물의 활성도를 유지하기 위해서 전술한 미네랄 용출이 용이한 광물과 원적외선이나 음이온을 방사하는 광물(11)을 충전한 미네랄공급기(10)을 설치하여 송풍기로부터 공기를 공급하여 폭기를 하면 배양액이 미네랄공급기(10)에 충전된 광물(11)의 층을 순환하면서, 순환펌프(4)로는 영구자석이나 전자석(5)과 자화탱크(7)의 자철광(8)의 자계를 순환하여 자화처리를 하므로서 미생물은 활발한 대사활동을 하는 상태로 활성이 유지되도록 한다.In addition, in the case of storing and using the cultured microorganism, as shown in FIG. 2, a culture medium storage tank 9 is provided, and in order to maintain the activity of the microorganisms in the culture medium storage tank 9, the above-mentioned minerals and far-infrared minerals are easy to dissolve. When the mineral feeder 10 filled with the minerals 11 emitting negative ions is installed to supply air from the blower, the aeration is circulated while circulating the layers of the minerals 11 filled in the mineral feeder 10. (4) by circulating the magnetic field of the permanent magnet or electromagnet (5) and the magnetite (8) of the magnetization tank (7) to the magnetization process to keep the activity in the state of active metabolic activity.

이상에서 전술한 바와 같이 본 발명에서는 생물학적악취처리, 하 ·폐수의 생물학처리, 양어장, 가축의 음용수, 식품공장 등에서 유용미생물배양의 경우 활성도가 높은 미생물이 배양되기 때문에 이들 분야에 널리 이용될 것으로 전망된다.As described above, the present invention is expected to be widely used in these fields because microorganisms having high activity are cultivated in the case of useful microbial culture in biological malodor treatment, biological treatment of sewage and wastewater, fish farms, drinking water of livestock, food factories, etc. do.

Claims (2)

농업분야, 환경분야, 식품분야, 양어장, 가축의 음용수처리 등의 분야에 풀브산(Fulvic acid) 함량이 많은 부식물질 펠렛트(Pellet)와 미네랄(Minerals) 용출이 용이한 감람석(橄欖石), 각섬석(角閃石), 흑운모(黑雲母), 진주석(珠石), 흑요석(黑曜石), 송지석(松脂石), 유문암(流紋岩)이나 대사이드(Dacite)질의 부석(浮石)과 같은 광물을 1종류이상 충전(充墳)하고, 원적외선(遠赤外線)이나 음이온을 방사하는 천매석(千枚石), 맥반석(飯石), 전기석(電氣石), 거정석(巨晶石), 의왕석(王石), 석영섬록암(石英閃綠岩), 귀보석(貴石), 태징석(泰澄石), 신명석(明石)과 같은 광물도 1종류이상 충전한 생물반응기(Bio-reactor ; 2)가 내장된 미생물반응조(1)에 배양액을 공급하면서 송풍기로부터 공기를 공급하면서 폭기를 하여 배양액을 생물반응기(2)에 충전된 부식물질 펠렛트(Pellet) 및 광물(3)의 층을 순환하면서 순환펌프(Pump ; 4) 토출측에 자속밀도(磁束密度)가 10,000가우스(Gauss)이상 착자된 영구자석이나 전자석(5)을 설치하고, 자화탱크(Tank ; 7)에는 자속밀도가 200가우스이상 착자된 자철광(8)을 충전한 자계(磁界)를 순환하면서 유용미생물을 배양하는 방법.Olivine, which is easy to dissolve corrosive pellets and minerals with high Fulvic acid content in agriculture, environment, food, fish farm, drinking water treatment of livestock, etc. Hornblende, biotite, mother-of-pearl One or more kinds of minerals, such as stone, obsidian, songji stone, rhyolite, and large-sided stone, are filled with far-infrared rays. Aphrodisiac, anemone stone, and elvan Stone, tourmaline, giant stone, Uiwang stone 王石, Quartz diorite, precious stones Stone, Tajing Stone, Shin Myeong-seok Minerals such as 明石 also aerated while supplying the culture medium to the microbial reactor (1) containing one or more types of bioreactors (2), and aeration while supplying air from the blower to the culture medium to the bioreactor (2) Permanent magnets or electromagnets (5) magnetized with magnetic flux densities of more than 10,000 Gauss at the discharge side of the circulating pump (Pump; 4) while circulating the packed corrosive pellets and the layers of minerals (3) Method for culturing useful microorganisms while circulating a magnetic field filled with magnetite (8) magnetized in the magnetization tank (Tank; 7) magnetic flux density of 200 gauss or more. 제1항에 있어서, 미생물배양액을 저장하였다가 사용하는 경우에는 도2에서와 같이 배양액 저장조(9)를 설치하고, 미생물의 활성도를 유지하기 위해서 전술한 미네랄 용출이 용이한 광물과 원적외선이나 음이온을 방사하는 광물(11)을 충전한 미네랄공급기(10)을 설치하여 송풍기로부터 공기를 공급하여 폭기를 하면 배양액이 미네랄공급기(10)에 충전된 광물(11)의 층을 순환하면서 순환펌프(4)로는 영구자석이나 전자석(5)과 자화탱크(7)의 자철광(8)을 순환하여 자화처리를 하므로서 미생물은 활발한 대사활동을 하는 상태로 활성이 유지되도록 하는 방법.The method according to claim 1, wherein in the case of storing and using the microbial culture medium, the culture medium storage tank 9 is installed as shown in FIG. 2, and the above-mentioned minerals and far-infrared rays or anions which are easy to elute the microorganisms are maintained in order to maintain the activity of the microorganisms. When the mineral supplier 10 filled with the minerals 11 to emit is installed and aeration is supplied by supplying air from the blower, the culture medium circulates the layers of the minerals 11 filled in the mineral supplier 10 while circulating the pump 4 Furnace is magnetized by circulating the permanent magnet or electromagnet (5) and the magnetization tank (8) of the magnetization process to keep the microorganism active in the state of active metabolic activity.
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KR20160059300A (en) * 2014-11-18 2016-05-26 강원대학교산학협력단 Method for production of medium for cultivation of porcine uterus epithelial cell
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