KR100458008B1 - Lactic acid bacteria for higher productivity of livestock - Google Patents
Lactic acid bacteria for higher productivity of livestock Download PDFInfo
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- KR100458008B1 KR100458008B1 KR10-2002-0075865A KR20020075865A KR100458008B1 KR 100458008 B1 KR100458008 B1 KR 100458008B1 KR 20020075865 A KR20020075865 A KR 20020075865A KR 100458008 B1 KR100458008 B1 KR 100458008B1
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- lactic acid
- acid bacteria
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- bacteria
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- 244000144972 livestock Species 0.000 title claims abstract description 15
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 title abstract description 42
- 241000894006 Bacteria Species 0.000 title abstract description 26
- 239000004310 lactic acid Substances 0.000 title abstract description 21
- 235000014655 lactic acid Nutrition 0.000 title abstract description 21
- 241000218492 Lactobacillus crispatus Species 0.000 claims abstract description 8
- 238000000034 method Methods 0.000 claims description 11
- 239000000546 pharmaceutical excipient Substances 0.000 claims 2
- 239000007787 solid Substances 0.000 claims 2
- 230000000694 effects Effects 0.000 abstract description 10
- 239000002253 acid Substances 0.000 abstract description 7
- 210000000941 bile Anatomy 0.000 abstract description 7
- 102000004190 Enzymes Human genes 0.000 abstract description 5
- 108090000790 Enzymes Proteins 0.000 abstract description 5
- 210000001035 gastrointestinal tract Anatomy 0.000 abstract description 4
- 235000015097 nutrients Nutrition 0.000 abstract description 4
- 230000028327 secretion Effects 0.000 abstract description 4
- 206010040007 Sense of oppression Diseases 0.000 abstract description 3
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 3
- 244000000010 microbial pathogen Species 0.000 abstract description 3
- 230000000052 comparative effect Effects 0.000 description 10
- 241000186660 Lactobacillus Species 0.000 description 7
- 229940039696 lactobacillus Drugs 0.000 description 7
- 239000006041 probiotic Substances 0.000 description 7
- 235000018291 probiotics Nutrition 0.000 description 7
- 239000003242 anti bacterial agent Substances 0.000 description 6
- 229940088710 antibiotic agent Drugs 0.000 description 6
- 244000005700 microbiome Species 0.000 description 6
- 238000007792 addition Methods 0.000 description 4
- 239000004382 Amylase Substances 0.000 description 3
- 102000013142 Amylases Human genes 0.000 description 3
- 108010065511 Amylases Proteins 0.000 description 3
- 244000063299 Bacillus subtilis Species 0.000 description 3
- 235000014469 Bacillus subtilis Nutrition 0.000 description 3
- 241000588724 Escherichia coli Species 0.000 description 3
- 108091005804 Peptidases Proteins 0.000 description 3
- 239000004365 Protease Substances 0.000 description 3
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 235000019418 amylase Nutrition 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 230000001965 increasing effect Effects 0.000 description 3
- 230000000529 probiotic effect Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 241000233866 Fungi Species 0.000 description 2
- 240000001929 Lactobacillus brevis Species 0.000 description 2
- 235000013957 Lactobacillus brevis Nutrition 0.000 description 2
- 241000607142 Salmonella Species 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 210000000936 intestine Anatomy 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 230000004584 weight gain Effects 0.000 description 2
- 235000019786 weight gain Nutrition 0.000 description 2
- 108020004465 16S ribosomal RNA Proteins 0.000 description 1
- WDMUXYQIMRDWRC-UHFFFAOYSA-N 2-hydroxy-3,4-dinitrobenzoic acid Chemical compound OC(=O)C1=CC=C([N+]([O-])=O)C([N+]([O-])=O)=C1O WDMUXYQIMRDWRC-UHFFFAOYSA-N 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 102000016938 Catalase Human genes 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 238000003794 Gram staining Methods 0.000 description 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 1
- 241001104358 Lactobacillus crispatus DSM 20584 = JCM 1185 Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 108010059712 Pronase Proteins 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 239000003674 animal food additive Substances 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 102000038379 digestive enzymes Human genes 0.000 description 1
- 108091007734 digestive enzymes Proteins 0.000 description 1
- 230000006806 disease prevention Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 235000021050 feed intake Nutrition 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000001937 non-anti-biotic effect Effects 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 210000004798 organs belonging to the digestive system Anatomy 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 238000009374 poultry farming Methods 0.000 description 1
- 235000013594 poultry meat Nutrition 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 1
- 238000010200 validation analysis Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
Abstract
본 발명은 가축 장내에서 분리한 유산균 락토바실러스 크리스파투스 계산(Lactobacillus crispatus Gyesan, 수탁번호 : KCTC-10326BP)에 관한 것으로, 가축의 체내에서 생존 가능한 내산성과 내담즙성을 가지고 있으며, 효소분비력도 우수한 것으로 나타나, 영양소 이용효율 증대에 기여할 수 있고, 병원성 미생물에 대한 억압력도 뛰어나 항생제 대체효과가 기대되는 가축 생산성 향상 유산균이다.The present invention relates to lactic acid bacteria Lactobacillus crispatus Gyesan (Accession No .: KCTC-10326BP) isolated from the intestinal tract, having acid resistance and bile resistance that can survive in the body of livestock, and enzyme secretion ability. It is excellent in lactic acid bacteria, which can contribute to the increase of nutrient utilization efficiency, and has excellent oppression against pathogenic microorganisms, and antibacterial replacement effect is expected.
Description
본 발명은 가축 장내에서 분리한 유산균 락토바실러스 크리스파투스 계산(Lactobacillus crispatus Gyesan, 수탁번호 : KCTC-10326BP)에 관한 것으로, 가축의 체내에서 생존 가능한 내산성과 내담즙성을 가지고 있으며, 효소분비력도 우수한 것으로 나타나, 영양소 이용효율 증대에 기여할 수 있고, 병원성 미생물에 대한 억압력도 뛰어나 항생제 대체효과가 기대되는 가축 생산성 향상 유산균이다.The present invention relates to lactic acid bacteria Lactobacillus crispatus Gyesan (Accession No .: KCTC-10326BP) isolated from the intestinal tract, having acid resistance and bile resistance that can survive in the body of livestock, and enzyme secretion ability. It is excellent in lactic acid bacteria, which can contribute to the increase of nutrient utilization efficiency, and has excellent oppression against pathogenic microorganisms, and antibacterial replacement effect is expected.
통상, 양계농가의 경영에 지속적인 해결과제가 질병의 차단, 계분 및 바닥깔짚에 의한 악취문제, 그리고 생산성의 향상이다.Normally, ongoing challenges in poultry farming are the prevention of disease, the bad smell caused by poultry and floor litter, and the improvement of productivity.
이러한 문제의 해결을 위하여 주로 이용되는 방법이 사료첨가제인 항생제와 생균제의 사용이다.The most commonly used method to solve these problems is the use of antibiotics and probiotics as feed additives.
상기 항생제는 그 효과면에서 가장 빠르고 만족할만한 효과를 얻을 수 있는 것으로, 설사방지와 성장촉진에 매우 유익하지만, 항생물질에 저항성이 강한 미생물 유도와 축산물에 대한 잔류 가능성 이외에도, 인간에 있어 병원성 미생물의 항생제에 대한 저항성 증진 등에 대한 문제가 야기된다.The antibiotic is the fastest and most satisfactory effect in terms of its effect, which is very beneficial for preventing diarrhea and promoting growth, but in addition to the induction of microorganisms resistant to antibiotics and the possibility of residue on livestock products, Problems arise, such as increased resistance to antibiotics.
이러한 이유로, 항생제를 대체할 수 있는 첨가제로서 생균제가 주로 이용되고 있는데, 생균제는 살아있는 미생물 혹은 비항생제적 물질로서 가축에 대하여 급여 시 장내 해로운 미생물을 감소시키고 성장을 촉진하며, 소화기관 미생물의 환경을 개선함으로써 사료의 가치를 증진시킬 수 있다.For this reason, probiotics are mainly used as additives to replace antibiotics. Probiotics are living or non-antibiotic substances, which reduce harmful microorganisms in the intestines when feeding livestock, promote growth, and improve the environment of digestive organ microorganisms. By improving, you can increase the value of your feed.
상기 생균제의 종류는 세균(Bacteria), 곰팡이(Fungi), 효모(Yeast)가 포함되며, 사용 목적에 따라서는 섭취용, 바닥 살포용, 분뇨 처리용으로 구분된다.Types of the probiotic include bacteria (Bacteria), fungi (Fungi), yeast (Yeast), depending on the purpose of use is divided into intake, floor spraying, manure treatment.
섭취용으로는 살아있는 생균 상태로 공급하는 것과 배양물 위주의 2 가지로 대별되는데, 전자에는 유산균류, 후자에는 효모, 황국균 등이 포함된다.There are two types of intakes, which are supplied in a living and live state, and mainly in culture. The former includes lactic acid bacteria, the latter includes yeast, and yellow bacteria.
유산균은 현재 섭취용으로서 가장 많이 이용되는 생균제의 하나로서, 유산구균과 유산간균이 있는데, 가축의 소화기관 전체에 걸쳐 존재하는 균으로, 유산을 생성하여 유해세균의 발육억제 및 정상적인 대사작용을 유지시켜 주며, 종류에 따라서는 여러 가지의 항생물질을 생산하기도 한다.Lactobacillus is one of the most commonly used probiotics for ingestion. Lactobacillus and Lactobacillus are bacteria that exist throughout the digestive tract of livestock. They produce lactic acid to inhibit the growth of harmful bacteria and maintain normal metabolism. It also produces various antibiotics depending on the type.
현재 이용되고 있는 유산균제의 종류는 매우 많으나, 가축에 대한 효과 검증은 차이가 매우 많다.There are many types of lactic acid bacteria currently used, but the effectiveness of livestock validation is very different.
그러한 이유 중 첫번째가 생균제적 가치유무이다.The first of these is the probiotic value.
특정 생균제가 역할을 다하기 위해서는 소화기관에서 생존할 수 있어야 하며, 동시에 숙주동물에 유익한 작용을 할 수 있어야 하는데, 생존여부가 불확실한 균이 많이 있다.In order for a specific probiotic to play a role, it must be able to survive in the digestive tract, and at the same time be able to benefit the host animal.
두번째가 적당한 급여방법이다.The second is the proper payment method.
유산균이 가축의 장내에 유입됨과 동시에 분비물과 원주균에 의해 생존이 위협받게 되는데, 이 때, 목표지점에서 최소한의 생존 미생물의 수가 유지되어야만 목적에 부합되는 역할을 할 수 있다.At the same time as lactic acid bacteria enter the livestock intestine, survival is threatened by secretions and columnar bacteria, which can serve their purpose only when the minimum number of surviving microorganisms is maintained at the target site.
따라서, 본 발명에서는 생체에서 생존과 유익한 활동을 할 수 있다고 판단되는 유산균을 개발함으로써, 가축의 체내에서 생존 가능한 내산성과 내담즙성을 가지고 있으며, 효소분비력도 우수한 것으로 나타나, 영양소 이용효율 증대에 기여할 수 있고, 병원성 미생물에 대한 억압력도 뛰어나 항생제 대체효과가 기대되는 가축 생산성 향상 유산균을 제공하고자 한다.Therefore, in the present invention, by developing lactic acid bacteria which are judged to be able to survive and beneficial activity in the living body, it has been shown to have acid resistance and bile resistance that can survive in the body of livestock, and also shows an excellent enzyme secreting ability, thereby increasing the nutrient utilization efficiency. It is expected to provide lactic acid bacteria that can contribute to livestock productivity, which can contribute to the antimicrobial and antibacterial effects.
이하, 본 발명을 실시예를 통하여 좀더 상세히 설명한다.Hereinafter, the present invention will be described in more detail with reference to Examples.
실시예 1(개발유산균의 특징)Example 1 (Characteristics of Developmental Lactobacillus)
1. 유산균의 형태(MRS broth에서 25시간 배양 시)1.Lactobacillus form (incubated for 25 hours in MRS broth)
* 세포의 형태 : 간균* Cell Form: Bacillus
* 운동성 : 없음* Mobility: None
* 포자 형성능 : 없음* Spore Formability: None
* 그람(Gram) 염색 : 양성* Gram staining: positive
2. 생리적 성질2. Physiological Properties
* 최적 생장온도 : 37-39℃* Optimum Growth Temperature: 37-39 ℃
* 최적 생육pH : 6.3-6.7* Optimum Growth pH: 6.3-6.7
* 산소에 대한 영향 : 통성혐기성* Effects on Oxygen: Breathable Anaerobic
* 젖산 생산유무 : +* Lactate production: +
3. 균주의 동정3. Identification of Strains
* 방법 : 16S rRNA partial sequencing* Method: 16S rRNA partial sequencing
* 유사도 분석결과 : Lactobacillus crispatus DSM 20584(Y17362)와 99.54%로서 가장 유사도가 높게 나타남* Similarity Analysis Results: Lactobacillus crispatus DSM 20584 (Y17362) was 99.54% and showed the highest similarity.
실시예 2(생화학적 특성)Example 2 (Biochemical Properties)
방법 1)Method 1)
내산성은 pH가 1,2,3,4로 조정된 PBS 2ml에 0.1ml씩 접종하여 37℃에서 4시간 동안 성장을 관찰하였다.Acid resistance was inoculated by 0.1ml in 2ml PBS adjusted to pH 1,2,3,4 and observed growth for 4 hours at 37 ℃.
내담즙성 조사는 37℃에서 24시간 동안 MRS broth에 배양된 균배양액을 37℃에서 6시간 동안 보관하며, 매 시간 660nm 상태에서 비색계로 흡광도를 측정하였다.Bile resistance irradiation was stored for 24 hours at 37 ℃ culture medium in MRS broth for 6 hours at 37 ℃, the absorbance was measured by a colorimeter at 660 nm every hour.
아밀라제의 활성에 대한 측정은 밀러(Miller, 1959)의 디니트로살리신산 (DNS, Sigma법), 프로테아제의 활성은 야나기다 등(Yanagida etc., 1986)의 방법을 응용하여 조사하였다.The activity of amylase was measured by Miller (1959), dinitrosalicylic acid (DNS, Sigma method), and the protease activity by applying the method of Yanagida et al. (1986).
개발 유산균과 대비하기 위하여 표준 균주로서 유산균 1종과 고초균 1종을 이용하였다.In order to prepare with lactic acid bacteria, lactic acid bacteria and Bacillus subtilis were used as standard strains.
결과 1)Result 1)
내산성 결과 pH 1과 2에서는 생존에 영향을 심하게 받지만, pH 3 이상에서는전혀 영향을 받지 않았다.Acid resistance results were significantly affected by survival at pH 1 and 2, but not at pH 3 or above.
내담즙성 결과 일정한 수효를 계속 유지하는 것으로 나타났다.Bile resistance results showed that they maintained a constant number.
전분 분해효소인 아밀라제와 단백질 분해효소인 프로테아제 활성은 개발 유산균이 모두 비교예보다 높게 나타났다.Starch amylase and protease activity were higher in the developed lactic acid bacteria than in the comparative example.
상기 실시예 1의 결과에서 가축의 pH가 가장 낮은 위의 경우 사료 섭취 시 pH가 4-5 정도이므로 섭취물과 첨가되어 위를 통과하더라도 생존이 가능한 것으로 나타났다.In the result of Example 1, when the pH of the livestock was the lowest, the pH of the feed was about 4-5.
또, 소화효소의 활성이 높은 것으로 사료영양소 이용효율 향상에 도움을 줄 수 있을 것으로 판단된다.In addition, the high activity of the digestive enzymes is believed to help improve feed nutrient utilization efficiency.
항병성 조사에서 유산균 배양액은 E.coli 08:K85:K99에 대하여 높은 항병성을 나타내었다.Lactobacillus cultures showed high anti-pathogenicity against E. coli 08: K85: K99.
<내산성><Acid resistance>
<내담즙성><Bile Resistance>
<효소활성>Enzyme activity
LB : Lactobacillus brevis KCCM 40399, BS : Bacillus subtilis KCTC 1666.LB: Lactobacillus brevis KCCM 40399, BS: Bacillus subtilis KCTC 1666.
<E. Coli 08:K85:K99에 대한 항병성><E. Antipathic against Coli 08: K85: K99>
LB : Lactobacillus brevis KCCM 40399, BS : Bacillus subtilis KCTC 1666.LB: Lactobacillus brevis KCCM 40399, BS: Bacillus subtilis KCTC 1666.
실시예 3(가축 사양시험)Example 3 (Livestock Spec. Test)
방법2)Method 2)
개발 유산균의 생체효과 반응을 검증하고자 육계를 대상으로 5주 동안 평사에서 급여 실험을 실시하였다.To verify the bioeffect response of the developed lactic acid bacteria, feeding experiments were conducted in broiler chickens for 5 weeks.
비교예는 유산균 무첨가구와 기 등록된 유산균 Lactobacillus crispatus avihen 1 이었다.Comparative Examples were lactic acid bacteria-free additions and Lactobacillus crispatus avihen 1 group registered.
<생산성 비교(0-5주령)Productivity comparison (0-5 weeks old)
<맹장내 미생물수><Number of microorganisms in the appendix
결과2)Result 2)
생산성 비교에서, 체중은 실시예가 무첨가 비교예에 비하여 88g의 높은 증체향상이 있었다.In the productivity comparison, the weight gain was higher by 88 g compared to the comparative example without addition.
또, 사료요구율도 개선되는 것으로 나타났는데, 일반적으로 체중이 증가할수록 사료요구율은 커지는 것으로 볼 때, 매우 우수한 결과이다.In addition, the feed demand rate was also improved. In general, as the body weight increases, the feed demand rate increases, which is a very good result.
또한, 기 출원된 유산균보다 뒤떨어지지 않은 우수한 유산균으로 나타났다.In addition, it appeared to be excellent lactic acid bacteria lagged behind the previously filed lactic acid bacteria.
맹장내 미생물에서도 실시예가 무첨가예에 비하여 유산균은 많고 살모넬라와 대장균은 감소한 것으로 나타났다.In the cecal microorganisms, the lactic acid bacteria and Salmonella and Escherichia coli were found to be more numerous than those of the Example.
한편, 본 발명의 유산균 락토바실러스 크리스파투스 계산(Lactobacillus crispatus Gyesan)은 2002년 8월 22일자로 부다페스트 조약하의 국제기탁기관인 KCTC에 기탁번호 KCTC-10326BP로 기탁하였다.On the other hand, Lactobacillus crispatus Gyesan (Lactobacillus crispatus Gyesan) of the present invention was deposited on August 22, 2002 to KCTC, an international depository organization under the Budapest Treaty under the accession number KCTC-10326BP.
이상 설명한 바와 같이, 본 발명에서는 생체에서 생존과 유익한 활동을 할 수 있다고 판단되는 유산균을 개발함으로써, 가축의 체내에서 생존 가능한 내산성과 내담즙성을 가지고 있으며, 효소분비력도 우수한 것으로 나타나, 영양소 이용효율 증대에 기여할 수 있고, 병원성 미생물에 대한 억압력도 뛰어나 항생제 대체효과가 기대되는 가축 생산성 향상 유산균으로서, 관련 분야에의 이용 및 응용이 기대된다 하겠다.As described above, in the present invention, by developing lactic acid bacteria which are judged to be able to survive and have beneficial activity in living bodies, they have acid resistance and bile resistance that can survive in the body of livestock, and also show excellent enzyme secretion ability, As a livestock productivity enhancing lactic acid bacterium that can contribute to increased efficiency and has excellent oppression against pathogenic microorganisms and is expected to replace antibiotics, it is expected to be used and applied in related fields.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5804179A (en) * | 1985-12-31 | 1998-09-08 | Research Corporation Technologies, Inc. | Lactobacillus compositions and methods for treating urinary tract infections |
| US6342366B1 (en) * | 1997-08-06 | 2002-01-29 | Proge Farm S.R.L. | Lactobacilli strains having inhibitory and/or microbicidal activity against pathogenic microorganisms and a method for inducing and keeping said activity in lactobacilli cultures |
| KR20020033643A (en) * | 1999-06-21 | 2002-05-07 | 브이에스엘 파마 리미티드 | Combination of lactic acid bacteria and its use for the prevention and/or treatment of infections and inflammatory conditions |
| KR20020072143A (en) * | 2001-03-09 | 2002-09-14 | 대한민국(관리부서:농촌진흥청) | Lactobacillus for improving weight gain and environmental condition in brolier |
| KR20020072409A (en) * | 2001-03-09 | 2002-09-16 | 대한민국(관리부서:농촌진흥청) | Lactobacillus for improving egg weight and environmental condition |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5804179A (en) * | 1985-12-31 | 1998-09-08 | Research Corporation Technologies, Inc. | Lactobacillus compositions and methods for treating urinary tract infections |
| US6342366B1 (en) * | 1997-08-06 | 2002-01-29 | Proge Farm S.R.L. | Lactobacilli strains having inhibitory and/or microbicidal activity against pathogenic microorganisms and a method for inducing and keeping said activity in lactobacilli cultures |
| KR20020033643A (en) * | 1999-06-21 | 2002-05-07 | 브이에스엘 파마 리미티드 | Combination of lactic acid bacteria and its use for the prevention and/or treatment of infections and inflammatory conditions |
| KR20020072143A (en) * | 2001-03-09 | 2002-09-14 | 대한민국(관리부서:농촌진흥청) | Lactobacillus for improving weight gain and environmental condition in brolier |
| KR20020072409A (en) * | 2001-03-09 | 2002-09-16 | 대한민국(관리부서:농촌진흥청) | Lactobacillus for improving egg weight and environmental condition |
Non-Patent Citations (1)
| Title |
|---|
| The Journal of Microbiology, 2001 39(2);146-148 * |
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