KR100453207B1 - Method and detector for detecting and identifying subtypes of human papilloma viruses - Google Patents

Abstract

The present invention provides a detector for simultaneously identifying and discriminating a human papilloma pathogen (HPV) subtype contained in a sample.

A carrier having a first portion and a second portion capable of loading the sample thereon; a first type of oligonucleotide loaded on the first portion of the carrier; and loaded on the second portion of the carrier. A second type of oligonucleotide, wherein the first and second types of oligonucleotides are hybridized with deoxyribonucleic acid (DNA) of the first and second types of HPV subtypes, respectively, At the same time, it provides a detector that can distinguish between HPV subtypes contained in the sample.

Description

METHODS AND DETECTOR FOR DETECTING AND IDENTIFYING SUBTYPES OF HUMAN PAPILLOMA VIRUSES}

The present invention relates to detectors and detection methods used for differentiating pathogens, and more particularly to detectors and detection methods used for differentiating multiple human papilloma pathogens (HPV).

Cervical cancer is the number one cause of death in Taiwanese women and at the same time the number one among teenage cancers among women. The mortality rate of cervical cancer patients in Taiwan is seven to eight times higher than that of developed countries, but it is the most easily detected cancer. If a man or woman who is already infected infects another person through sexual activity, the woman may develop cervical cancer due to cytopathy, and the man may develop a verruca. Cellular aberrations prior to cervical cancer have been shown to be five to 10 years before cervical cancer occurs by medical research. The cervix must first develop a cervical intratraepithelial neoplasm and then develop into invasive cervical carcinoma. In order to reduce the incidence of cervical cancer, cervical pap smears are widely encouraged, so that bilateral cervical cells are detected before metastasis to cervical cancer and are treatable early. The accuracy of the fab smear is between about 60 and 70 percent, ie about 30 to 40 percent of the pre-cancerous changes in cervical cancer. Many studies also indicate that more than 95% of cervical cancers have been infected with the Human Papilloma Virus (HPV). Therefore, cervical cancer can be seen as an "infectious cancer," and if it is possible to detect Fab smear and human papilloma pathogens at the same time, it is possible to significantly increase the detection rate of cervical cancer.

Therefore, the present applicant, in cooperation with Taiwan's Radong Fraternity Hospital, investigated uterine cervical cancer epidemic in combination with polymerase chain reaction and nucleotide sequence technology by means of fab smear and HPV detection. The total number of examinees was 2424, and the age was 16-18 years old. The results are shown below.

1) 1.9% (37/1963) of women show abnormalities in cytology smears.

2) 12.7% (244/1926) of women with normal smear show HPV infection.

3) In women with smear abnormalities, the HPV prevalence was 51.4% (19/37), and there was a positive relationship according to the severity of smear abnormalities (non-specific squamous cell 23.1%, low epithelial cell variability). 47.1%, highly epithelial cell mutation 75%).

4) 29 types of HPV subtypes were found. 52, 58, 53, 70, 16, 18, 68, 33, 66, 35, 37, 54, 59, 67, 72, 69, 82, 39, 31, 32, HLT 7474-S, 6, CP8061, 62, CP8304, 44, 11, 61, 74, of which 52, 58, 69, 70 were atypical squamous cells, 51, 58, 69, 70 were at low epithelial cells, 16, were at high epithelial cells. 18, 33, 52, 53, 82, CP8061 have been found.

In contrast, the conventional HPV detection tool is a Hybrid Capture II HPV DNA microplate assay tool manufactured by DIGENE Co., and the high risk group HPV subtypes among the HPV subtypes that can be detected are 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 68, and the low-risk HPV subtypes are 6, 11, 42, 43, 44, and 16 in total.

As can be seen from the comparison of the results of the investigation of the cervical cancer epidemic, the specimen of the multi-subtype mixed infection of the human papilloma pathogen cannot simultaneously discriminate various pathogen subtypes in the prior art, and the conventional HPV detection tool is also made in Taiwan. Does not apply to women. In addition, since the result detected by the conventional HPV detection tool only indicates whether the sample is a high-risk or low-risk pathogen infection, it is necessary to carefully consider whether it is false positive or not. The distinction between high and low risk groups does not reveal the HPV subtype. On the other hand, there is no recognition of an effective sample if it is a false negative.

Accordingly, the present applicant has made many tests and studies in consideration of the deficiencies of the prior art, and finally devised a "detector and detection method for discriminating multiple human papilloma pathogen subtypes" of the present invention.

It is an object of the present invention to provide a detector for simultaneously identifying and discriminating human papilloma pathogen (HPV) subtypes contained in a specimen.

Another object of the present invention is to provide a detection method for simultaneously identifying and discriminating a human papilloma pathogen (HPV) subtype contained in a specimen.

A first invention (corresponding to claim 1) of the present invention is a detector for simultaneously identifying and discriminating a human papilloma pathogen (HPV) subtype contained in a specimen.

A carrier having a first portion and a second portion capable of loading the sample thereon;

A first type of oligonucleotide loaded on the first portion of the carrier,

A second type of oligonucleotide loaded on the second portion of the carrier,

Among them, the oligonucleotides of the first and second species are mixed with deoxyribonucleic acid (DNA) of the HPV subtypes of the first and second species, respectively, to simultaneously identify the HPV subtypes contained in the sample. And differentiating.

The second invention (corresponding to claim 2) is a detector for simultaneously identifying and discriminating HPV subtypes contained in the specimen of the first invention, that is, claim 1, characterized in that the material of the carrier is nylon or glass. do.

A third invention (corresponding to claim 3) is a detector for simultaneously identifying and discriminating HPV subtypes contained in a specimen of the first invention, that is, claim 1, wherein the first kind of HPV subtypes and the first Two HPV subtypes are selected from 36 HPV subtypes, and the base sequence of the oligonucleotide of the first type is the nucleotide sequence of the oligonucleotide group corresponding to the HPV subtype of the first species and its complementary base sequence. And the nucleotide sequence of the oligonucleotide of the second kind is selected from the one nucleotide sequence in the corresponding oligonucleotide group and its complementary nucleotide sequence.

A fourth invention (corresponding to claim 4) is a detector for simultaneously identifying and discriminating HPV subtypes contained in the first invention, that is, the sample described in claim 1, wherein the detector is an oligonucleotide test piece.

A fifth invention (corresponding to claim 5) is a method for detecting and discriminating the presence of a human papilloma pathogen (HPV) in a specimen,

Providing a first detection reagent comprising a first type of oligonucleotide and a second detection reagent comprising a second type of oligonucleotide (the first and second types of oligonucleotides are each Can be hybridized with deoxyribonucleic acid (DNA) of one and second HPV subtypes),

Hybridizing the DNA and the oligonucleotide in the sample;

Removing DNA that cannot hybridize with these oligonucleotides, and simultaneously identifying and discriminating these HPV subtypes contained in the sample.

A sixth invention (corresponding to claim 6) is the fifth invention, i.e., a method for detecting and discriminating the presence of human papilloma pathogens (HPV) in a sample according to claim 5, wherein the deoxyribo in the sample The nucleic acid (DNA) is characterized in that the product of the polymerase chain reaction.

A seventh invention (corresponding to claim 7) is a method for detecting and discriminating the presence of a human papilloma pathogen (HPV) in a fifth invention, i.e., a sample according to claim 5, wherein the deoxyribonucleic acid in the sample DNA is characterized in that it contains a signal material.

In the eighth invention (corresponding to claim 8), in the seventh invention, that is, in the method of detecting and discriminating the presence of human papilloma pathogens (HPV) in the sample described in claim 5,

The signal material is biotin,

The identification and discrimination method is also characterized in that it comprises the step of performing the reaction of the biotin and protein alkaline phosphatase (alkaline phosphatase).

In the ninth invention (corresponding to claim 9), in the seventh invention, i.e., a method for detecting and discriminating the presence of human papilloma pathogens (HPV) in a sample according to claim 7,

The signal material is a fluorescent material,

The fluorescent material is characterized by Cyanine5.

The tenth invention (corresponding to claim 10) relates to a method for detecting a specific human papilloma pathogen (HPV) subtype in a specimen,

Providing an oligonucleotide that is capable of hybridizing with a single deoxyribonucleic acid (DNA) sequence specific to said specific human papilloma pathogen (HPV) subtype,

Hybridizing deoxyribonucleic acid (DNA) and the oligonucleotide in the sample;

Removing deoxyribonucleic acid (DNA) in the test reagent that cannot be hybridized with the oligonucleotide;

Detecting the remaining deoxyribonucleic acid (DNA) and indicating whether the sample contains the specific human papilloma pathogen (HPV) subtype,

Among them, the gene number of the specific human papilloma pathogen (HPV) subtype and its unique single deoxyribonucleic acid (DNA) are contiguous to the position of the gene and selected from the specific 36 human papilloma pathogen (HPV) subtypes. do.

The eleventh invention (corresponding to claim 11) is a composition for detecting a human papilloma pathogen (HPV) subtype contained in a sample,

A base sequence for detecting the presence of the human papilloma pathogen (HPV) subtype comprises an oligonucleotide capable of hybridizing with a deoxyribonucleic acid (DNA) sequence specific to the human papilloma pathogen (HPV) subtype, among them,

The human papilloma pathogen (HPV) subtypes are HPV 26, HPV 32, HPV 37, HPV 53, HPV 61, HPV 62, HPV 66, HPV 67, HPV 69, HPV 70, HPV 72, HPV 74, HPV 82, HPVCP 8061, HPVCP 8304, HPVL1AE 5, HPVMM 4, HPVMM 7 and HPVMM 8.

1 illustrates a first embodiment of a detector for simultaneously identifying and discriminating HPV subtypes contained in a sample;

FIG. 2 shows a second embodiment of a detector for simultaneously identifying and discriminating HPV subtypes contained in a sample; FIG.

3 (a) and 3 (b) illustrate a third embodiment of a detector for simultaneously identifying and discriminating HPV subtypes contained in a specimen.

Figure 4 (a) (b) (c) is a view showing the results of discriminating the HPV subtype contained in the sample by applying the third embodiment.

Fig. 5 shows the results of discriminating HPV subtypes included in a sample by applying the third embodiment.

6 (a) and 6 (b) illustrate a fourth embodiment of a detector for simultaneously identifying and discriminating HPV subtypes included in a specimen.

7 (a) and 7 (b) show results obtained by discriminating HPV subtypes included in a sample by applying the fourth embodiment.

In Fig. 1, oligonucleotide test piece 10 is a first embodiment for simultaneously identifying and discriminating a human papilloma pathogen (HPV) subtype contained in a specimen. The carrier 11 is a nylon film, on which a dot array 12 is fixed. Each dot of dot array 12 is an oligonucleotide (15-30 nucleotides) probe pin, and each oligo nucleotide probe pin can hybridize with deoxyribonucleic acid (DNA) of the HPV subtype to a limited extent.

Applicant arranges nucleotide sequences of 97 known HPV subtypes by nucleotide sequencing control software, and then selects HPV subtypes previously popular in Taiwan, and hybridizes oligonucleotides of the HPV subtypes limited. Possible base sequences (Tables 1 to 36) were designed. Each table is an oligonucleotide sequence employed for a particular HPV subtype, and is used to specifically hybridize that particular HPV subtype.

Each oligonucleotide sequence on the carrier 11 is selected with reference to Tables 1-36. For example, the base sequence of the single oligonucleotide probe pin on the carrier 11 may employ the single base sequence of the numbers M1101 to M1124 (see Table 1) in order to hybridize HPV subtype II (HPV 11).

Table 1. Oligonucleotide Sequences That Can Hybrid with HPV 11 and Corresponding HPV 11 Gene Locations

SEQ ID NO: 5 '-> 3' position consecutive to HPV 11 M1101 (1) ATCTGTGTCTAAATC 6799-6813M1102 (2) TCTGTGTCTAAATCTGCTAC 6800-6819M1103 (3) ATCTGTGTCTAAATCTGCTACATACA 6799-6824M1104 (4) TGCATCTGTGTCTAAATCTG 6796-6815M1105 (5) AAATCAACTACATAATAAATAAA6ATAATAAA6TACTA (8) TAGCATTACATTATCTGCAGAAG 6895-6917M1109 (9) TCCTTCTGTTTTGGAGGAC 6943-6961M1110 (10) TTTATCGCCTCCACCAAATGGTACAC 6973-6998M1111 (11) TTATAGATATGTACAGTCACAGGCC 7009-7033M1112 (12) ACCCACACCTGAAAAAGAAAAAC 7048-7070M1113 (13) GGCGGATGCTCATTATGCGACTG 1044-1066M1114 (14) ATATGTAAGTCCTATAAGCAATGTAG 1101-1126M1115 ( 15) CTAATGCAGTAGAAAGTGAGATAAGT 1127-1152M1116 (16) CACGGTTAGACGCCATTAAACTTACA 1154-1179M1117 (17) CACAGCCAAAAAAGGTAAAGCGACGG 1181-1206M1118 (18) GCAACGCAGGTAGAGAAACATGGCGA 1264-1289M1119 (19) GAAAATGGGGGAGATGGTCAGGAAAGGG 1294-1323ACM1120 (20) GACACAGGGAGGGACATAGAGGGTGA 1321-1346M1121 (21) ACATAGAGAGGCGGAAGCAGTAGACG 1356-1381M1122 (22 ) ACAGCACCCGAGAGCATGCAGACACA 1382-1407M1123 (23) TCAGGAATATTAGAATTACTAAAATG 1408-1433M1124 (24) GCTGTCATTTGTTGATTTA 1482-1500

Table 2. Oligonucleotide Sequences That Can Hybrid with HPV 11 and Corresponding HPV 11 Gene Locations

SEQ ID NO: 5 '-> 3' position consecutive to HPV 16 M1601 (25) TATGTCATTATGTGCTGCCA 6659-6678M1602 (26) GTGCTGCCATATCTACTTCA 6670-6689M1603 (27) TGCCATATCTACTTC 6674-6688M1604 (28) TATCTACTTCAGAAACTACA 6679-6698M1605 (29) CTACTTCAG682A703A703A703AC703 (32) TTCCACTATTTTGGAGGAC 6821-6839M1609 (33) TCTACAACCTCCCCCAGGAGGCACAC 6851-6876M1610 (34) TTATAGGTTTGTAACCCAG 6887-6905M1611 (35) ACATACACCTCCAGCACCT 6923-6941M1612 (36) CCTTAAAAAATACACT 6956-6971M1613 (37) AGCAAAACAACATAGAGATGCAG 1089-1111M1614 (38) ACTTAGTGATATTAGTGGATGTGTAG 1145-1170M1615 ( 39) TAGTCCTAGATTAAAAGCTATATGTATAG 1181 -1210AM1616 (40) GAAAAACAAAGTAGAGCTGCAAAAAG 1209-1234M1617 (41) CTCAGCAGATGTTACAGGTAGAAGGG 1285- 1310M1618 (42) CCATGAGACTGAAACACCATGTAGTC 1313- 1338M1619 (43) GGGGGTGGTTGCAGTCAGTACAGTAG 1356- 1381M1620 (44) GTGGGGGAGAGGGTGTTAGTGAAAGA 1387- 1412M1621 (45) CTATATGCCAAACACCACTTACAAAT 1417-1442M1622 (46 ) GTTATACGGGGTGAGTTTTTCAGAAT 1502-1527

Table 3. Oligonucleotide Sequences That Can Hybrid with HPV 18 and Corresponding HPV 18 Gene Locations

SEQ ID NO: 5 '-> 3' position consecutive to HPV 18 M1801 (47) TTCTACACAGTCTCC 6650-6664M1802 (48) CAGTCTCCTGTACCTGGGCA 6657-6676M1803 (49) AGTCTCCTGTACCTGGGCAA 6658-6677M1804 (50) TCTCCTGTACCTGGGCAATATGA 6660-6682M1805 (51) CTGTACCTGGG679 (54) TAGCAGTATTTTAGAGGAT 6800-6818M1809 (55) TGTTCCCCCCCCCCCAACTACTAGTT 6830-6855M1810 (56) ATATCGTTTTGTACAATCTGTT 6866-6887M1811 (57) GGATGCTGCACCGGCTGAA 6905-6923M1812 (58) CTATGATAAGTTAAAG 6935-6950M1813 (59) GGTCCACAATGATGCACAAGTGT 1135-1157M1814 (60) CACAGAAAACAGTCCATTAGGGGAGC 1192-1217M1815 ( 61) GCTGGAGGTGGATACAGAGTTAAGTC 1219-1244M1816 (62) AGTGGGCAGAAAAAGGCAAAAAGGCGG 1271-1300CTGM1817 (63) CACAGATTCAGGTAACTACAAATGGC 1347-1372M1818 (64) CAATGTATGTAGTGGCGGCAGTACGG 1384-1409M1819 (65) GACAACGGGGGCACAGAGGGCAACAA 1418-1443M1820 (66) GTAGACGGTACAAGTGACAATAGCAA 1451-1476M1821 (67) CCACAATGTACCATAGCACAATTAAA 1493-1518M1822 (68 ) CACATATGGGCTATCATTTACAGATT 1573-1598

Table 4. Oligonucleotide Sequences That Can Hybrid with HPV 26 and Corresponding HPV 26 Gene Locations

SEQ ID NO: 5 '-> 3' position consecutive to HPV 26 M2601 (69) TAGTACATTATCTGCAGCAT 6619-6638M260270) ATTATCTGCAGCATC 6625-6639M2603 (71) TGCAGCATCTGCATCCACTC 6631-6650M2604 (72) GCATCTGCATCCACTCCATTTAAA 6635-6658M2605 (73) CTCCATTTAAACCAT741MAC ) ACTAACCTTACCTCCCACTGCTAGTT 6805-6830M2609 (77) CTATAGGTTTATTAAAAACTCT 6841-6862M2610 (78) TAACGCCCCTCCTGTGCCA 6880-6898M2611 (79) AAAACAGGCAAATACAAAGGCAG 1093-1115M2612 (80) CTAGGTAGTCAGAACAGCCCGTTGCA 1139-1164M2613 (81) GCGACAGTCAGCAGAATACACACCAAGTA 1194-1223AM2614 (82) CAAAAGGAGAGCCGTGGACAGTGTAC 1237-1262M2615 (83) CCGTACAGGTAGATAAACAATATGAA 1305-1330M2616 (84) GCCTAGTGTGTGTAGTCAGGGGGGG 1345-1369M2617 (85) GCCTCAGTGGAAGATATCGATGTAGA 1376-1401M2618 (86) CAGTGTTACACAAATATGTGAATTAT 1414-1439M2619 (87) CAGTATATGGTAA

Table 5. Oligonucleotide Sequences That Can Hybrid with HPV 31 and Corresponding HPV 31 Gene Locations

SEQ ID NO: 5 '-> 3' position consecutive to HPV 31 M3101 (88) TGCAATTGCAAACAG 6592-6606M3102 (89) GCAATTGCAAACAGTGATAC 6593-6612M3103 (90) CAATTGCAAACAGTGATACT 6594-6613M3104 (91) GCAAACAGTGATACTACATTTAA 6599-6621M3105 (92) CTACATTTAATT62AT66ACTATATAA6AAATAGATAA6A6 (95) ATTGACCACACCTCCCTCAGGTTCTT 6769-6794M3109 (96) CTATAGGTTTGTCACCTCACAG 6805-6826M3110 (97) AACTGCCCCCCAAAAGCCC 6844-6862M3111 (98) AGCGGAGGAACATGCAGAGGCTG 1083-1105M3112 (99) TTTAAGTGATATTAGTAGTTGTGTGG 1140-1165M3113 (100) AGTCCACGGTTAAAAGCTATATGCATAGA 1177-1206AM3114 (101) GACTCTTTGAACTTCCAGACAGCGGG 1232-1257M3115 ( 102) GCAGCAGATGGTACAGGTAGAGGAGC 1281-1306M3116 (103) AATGGTAGTGACGGGACACATAGTGA 1327-1352M3117 (104) CCAACACGTAATATATTGCAAGTGTT 1369- 1394M3118 (105) GCAATGGTAAAGCTGCTATGTTAGGT 1403-1428M144

Table 6. Oligonucleotide Sequences That Can Hybrid with HPV 32 and Corresponding HPV 32 Gene Locations

SEQ ID NO: 5 '-> 3' position consecutive to HPV 32 M3201 (107) TGCTACTGTAACAACTGAAG 6906-6925M3202 (108) GCTACTGTAACAACTGAAGA 6907-6926M3203 (109) TACTGTAACAACTGA 6909-6923M3204 (110) ACTGTAACAACTGAAGACAC 6910-6929M3205 (111) CAACTGAAGACACATACAAGTC6917-6938AT (114) TGTAGCTCCACCGCCCTCTGGTACTT 7083-7108M3209 (115) TTATAGATTTGTGCAGTCTCAG 7119-7140M3210 (116) TAAGGTAACAGCACCTGAA 7158-7176M3211 (117) TTTTTCTGACTATTCA 7188-7203M3212 (118) AGCACATGCAGATAAGGAGGCAG 1062-1084M3213 (119) GGCAGTCCATATGAAAGTCCCGCCAGTGA 1111 -1140TM3214 (120) GAGCTAAGCCCTAGGCTTGGTGGATT 1162-1187M3215 ( 121) GGGGCCAAACGACGACTATTTCAATC 1210-1235M3216 (122) GCAGGAACAGGTAGAAAATGGACATG 1284-1309M3217 (123) GTGTACATGGGGTGCAGGAAAATCAG 1352-1377M3218 (124) GCCTACAACAAGGGTGGTGGAATTGC 1395-1420M3219 (125GTGT37GGTGT37GGTGT37

Table 7. Oligonucleotide Sequences That Can Hybrid with HPV 33 and Corresponding HPV 33 Gene Locations

SEQ ID NO: 5 '-> 3' position consecutive to HPV 33 M3301 (127) TATGCACACAAGTAACTAGT 6624-6643M3302 (128) CACACAAGTAACTAG 6628-6642M3303 (129) ACAAGTAACTAGTGACAGTA 6631-6650M3304 (130) GTAACTAGTGACAGTACATATAA 6635-6657M3305 (131) GTACATATAAAAATAGAATAGAATAGAATAGAAT6AAA741AAAATAAAATAGAATAGA6 (134) TTTAACACCTCCTCCATCTGCTAGTT 6805-6830M3309 (135) CTATAGGTTTGTTACCTCTCAG 6841-6862M3311 (136) AACAGTACCTCCAAAGGAA 6880-6898M3312 (137) CTTAGGTAAATATACA 6910-6925M3313 (138) AGGGGAGGATGATTTAAATGCTG 1100-1122M3314 (139) GCATGTTCACAAAGTGCTGCGGAGGA 1149-1174M3315 (140) GATCGTGCTGCAAACCCGTGTAGAAC 1182-1207M3316 ( 141) GAATGCACATACAGAAAACGAAAAATAG 1227-1256ATM3317 (142) AAATAGATGAGCTAGAAGACAGCGGA 1249-1274M3318 (143) GGTACAACAGGTAGAAAGTCAAAATG 1307-1332M3319 (144) GACTTAGAATCTAGTGGGGTGGGGGA 1350-1357M3320 (145) CTGTGAGACAAATGTAGATAGCTGTG 1391-1416M3321 (146) CGTTGCAGGAAATTAGTAATGTTCTA 1426-1451M3322 (147) GAGGCCTATGGAATAAGTTTTAT 1494-1516

Table 8. Oligonucleotide Sequences That Can Hybrid with HPV 35 and Corresponding HPV 35 Gene Locations

SEQ ID NO: 5 '-> 3' position consecutive to HPV 35 M3501 (148) TCTGCTGTGTCTTCTAGTGA 6612-6631M3502 (149) TGCTGTGTCTTCTAG 6614-6628M3503 (150) GTGTCTTCTAGTGACAGTAC 6618-6637M3504 (151) CTTCTAGTGACAGTACATATAAA 6622-6644M3505 (152) GTACAT506AAA66GA53MACACAA6AGAATA 6634ACTA (155) CCTTACACCACCGCCTTCTGGTACCT 6791-6816M3509 (156) ATATCGCTATGTAACATCACAG 6827-6848M3510 (157) ACCCAGTGCACCAAAACCT 6866-6884M3511 (158) GGAGCAAACACACAAAGAGGCTG 1089-1111M3512 (159) GCAGCGTGAGCTTATGTGTTAATAATAAC 1151-1180AM3513 (160) GTCCACGTTTAAAAGCTATTTGCA 1184-1207M3514 (161) CGATTATTTGAACTACCAGACAGCGG 1237-1262M35l5 ( 162) GAGATACAACAGGTAGAGGGGCATGA 1291 -1316M3516 (163) GGGCAGTGGGGATAGTATAACCTCTA 1338-1363M3517 (164) GACATGATGAGACTCCAACGCGAGAC 1376-1401M3518 (165) CTAAAATGTAGTAATGCAAACGCAGC 1414-1439AACTATG

Table 9. Oligonucleotide Sequences That Can Hybrid with HPV 37 and Corresponding HPV 37 Gene Locations

SEQ ID NO: 5 '-> 3' position consecutive to HPV 37 M3701 (167) TGTCTACTGACAATG 6782-6796M3702 (168) TGTCTACTGACAATGGCGAA 6782-6801M3703 (169) TGACAATGGCGAAGTTACAG 6789-6808M3704 (170) GACAATGGCGAAGTTACAGA 6790-6809M3705 (171) ACAGAGATA-6TACATATATA (174) TTCTGGTATATTGGAAGAG 6933-6951M3709 (175) ATTTGTACCTACTCCAGATAATTCAG 6963-6988M3710 (176) TTATAGGTACATTAATTCAAAG 6999-7020M3711 (177) TGCAGTTGTTGAAAAAGAA 7038-7056M3712 (178) CTTTGCAAAATATA

Table 10. Oligonucleotide Sequences That Can Hybrid with HPV 39 and Corresponding HPV 39 Gene Locations

SEQ ID NO: 5 '-> 3' position consecutive to HPV 39 M3901 (179) CTCTATAGAGTCTTC 6677-6691M3902 (180) TAGAGTCTTCCATACCTTCT 6682-6701M3903 (181) ATAGAGTCTTCCATACCTTC 6681 -6700M3904 (182) GTCTTCCATACCTTCTACATATG 6686-6708M3905 (183) CTACATATCT-6TCTACTACTACTACTACTACTACTACTACTACTACTACTACTACTACTACTACTACTACTACTACTACTACTACTACTATACTATACTATACTACACTACTACTACTATACTATACACTACTACTATACTACTATC6A (186) TGTAGCTCCTCCACCATCTGCCAGTT 6857-6882M3909 (187) TTACAGATACCTACAGTCTGCA 6893-6914M3910 (188) GGATGCTCCAGCACCTGAA 6932-6950M3911 (189) ATATGACGGTCTAAAG 6962-6977M3912 (190) GGCCCAAAGGGATGCACAAGCAG 1149-1171M3913 (191) CAGACAGCAGTGGCGACACTAGACCGTAT 1196-1225GM3914 (192) GTAGGCAGGAATACCAGGGGAACAC 1234-1258M3915 ( 193) GCAGTACGCAGGCAACACAAACGGTG 1283-1308M3916 (194) GGAGGAGGTAACTGTAGCAACTAATA 1362-1387M3917 (195) CATGGCGGCAGTGTACGGGAGGAGTG 1411-1436M3918 (196) GGATAGTGCTATAGATAGGAAAC 1446-1471M39TCACTCA

Table 11. Oligonucleotide Sequences That Can Hybrid with HPV 40 and Corresponding HPV 40 Gene Locations

SEQ ID NO: 5 '-> 3' position consecutive to HPV 40 M4401 (199) TGCCACTACACAGTC 6719-6733M4402 (200) CTACACAGTCCCCTCCGTCT 6724-6743M4403 (201) TGCCACTACACAGTCCCCTC 6719-6738M4404 (202) CAGTCCCCTCCGTCTACATATA 6729-6750M4405 (203) CTACATATACTAGTGAACMGT (206) GTTGTCGCCGCCCCCAAATGGTACCT 6899-6924M4409 (207) ATACAGATATGTGCAGTCCCAG 6935-6956M4410 (208) GCCACCCCCTGAAAAGGCA 6974-6992M4411 (209) CTATGCAAAATTAAGT 7004-7019M4412 (210) GGCGGATGCTCATTATGCGGCTG 1038-1060M4413 (211) GGTAGTCCATATGTTAGTCCTTTAAGTAA 1087-1116TM4414 (212) CACGGCTGGACGCTATAACATTAAGT 1148-1173M4415 ( 213) GACGGCTGTTTGACAGACCAGAATTA 1196-1221M4416 (214) GCTGAAACGCAGGTAGAGAGAAATGG 1255-1280M4417 (215) GGGAGGTGGACAAGGAAGGGACACAG 1299-1324M4418 (216) GGAAGTGGAGACACATAGCA-1GA-1AGAGAATATAATAACATAACATAACATAACATAACATAACATAACATAACATAACATAACATAACATAACATAACATAACATAACATAACATAACATAACATAACATAACATAACAA

Table 12. Oligonucleotide Sequences That Can Hybrid with HPV 45 and Corresponding HPV 45 Gene Locations

SEQ ID NO: 5 '-> 3' position consecutive to HPV 45 M4501 (219) TGCCTCTACACAAAATCCTG 6651-6670M4502 (220) CTCTACACAAAATCC 6654-6668M4503 (221) ACAAAATCCTGTGCCAAGTA 6660-6679M4504 (222) CAAAATCCTGTGCCAAGTAC 6661-6680M4505 (223) AATCCTG504664-TGAACMTAACACCAC6ATCCC (226) TAGTAGTATATTAGAAAAT 6804-6822M4509 (227) TGTCCCTCCACCACCTACTACAAGTT 6834-6859M4510 (228) ATATCGTTTTGTGCAATCAGTT 6870-6891M4511 (229) GGATACTACACCTCCAGAA 6909-6927M4512 (230) AGTTCAGAATGATGCACAGGTGT 1135-1157M4513 (231) GCAGCTAAGTGTGGATACGGATCTAAGT 1216-1245CCM4514 (232) CAAGAAATTTCATTAAATAGTGGGCA 1253-1278M4515 ( 233) ACGGTTGTTTACAATATCAGATAGTG 1294-1319M4516 (234) CATAGTACACAAAGTAGTGGTGGGGA 1397-1422M4517 (235) GACAATGCAGAAAATGTAGATCCGCA 1430-1455M4518 (236) GGAGCTATTACAAGCAAGTAACAAAA 1477-1502M45ATGAGA

Table 13. Oligonucleotide Sequences That Can Hybrid with HPV 51 and Corresponding HPV 51 Gene Locations

SEQ ID NO: 5 '-> 3' position consecutive to HPV 51 M5101 (239) CACTGCCACTGCTGCGGTTT 6555-6574M5102 (240) TGCCACTGCTGCGGT 6558-6572M5103 (241) CACTGCTGCGGTTTCCCCAA 6561-6580M5104 (242) CCACTGCTGCGGTTTCCCCA 6560-6579M5105 (243) CTGCGGTTTCCCCAACATTTAC 6566-6587M5106 (244) CAACATTTACTCCAAGTAAC 6578-6597M5107 (245) TAAAATTACTTTAACTACAGAGG 6657-6679M5108 (246) TCCTACCATTCTTGAACAG 6705-6723M5109 (247) ATTAACATTACCTCCGTCTGCTAGTT 6735-6760M5110 (248) ATATAGGTTTGTTAGAAATGCA 6771-6792M5111 (249) GGACACCCCTCCACAGGCT 6810-6828M5112 (250) TTTGGCCAAATATAAA 6840-6855M5113 (251) ATTACAGGCAAACAAAGAGGCTG 1092-1114M5114 (252) GCGAAGCAGCCCATTAGGAGACATTACA 1149-1178AAM5115 ( 253) CCATAGTCAGGCAAACGAGTCACAAG 1197-1222M5116 (254) AGATTACTGGACAGTTATCCGGACA 1231-1255M5117 (255) CAGGTAGATGGGCAACATGGCGGTTC 1300-1325M5118 (256) AGCTGTGCAAATGTAGAACTAAACAG 1384-1409M51TG (257ATAATGA477GT502GT

Table 14. Oligonucleotide Sequences That Can Hybrid with HPV 52 and Corresponding HPV 52 Gene Locations

SEQ ID NO: 5 '-> 3' position consecutive to HPV 52 M5201 (258) TGAGGTTAAAAAGGA 6695-6709M5202 (259) TGAGGTTAAAAAGGAAAGCA 6695-6714M5203 (260) GAGGTTAAAAAGGAAAGCAC 6696-6715M5204 (261) TTAAAAAGGAAAGCACATAT 6700-6719M5205 (262) AAAGGAAATCAAAAAAAAAAAAACATATAAAACACATATA (265) TGCCACTATTTTAGAGGAC 6839-6857M5209 (266) CCTTACCCCACCACCGTCTGCATCTT 6869-6894M5210 (267) ATACAGATTTGTCACTTCTACT 6905-6926M5211 (268) AAACACACCACCTAAAGGA 6944-6962M5212 (269) TTTAAAGGACTATATG 6974-6989M5213 (270) AGGGGAGGATGATTTACATGCTG 1085-1107M5214 (271) GCAGTCCGGAAAGTGCTGGGCAAGATGG 1135-1164TGM5215 ( 272) GGTAGTCCGCGTGCAAAACACATTTG 1176-1201M5216 (273) CCAAAACGCAAACCATGTCACGTAGA 1224-1249M5217 (274) CAAAATGGCGACTGGCAAAGTA 1311-1332M5218 (275) GCTAGTAATTCAGATGTAAGTTGTAC 1359-1384M5219 (276) GAGGAAAATAGTAATAGAACGCTAAA 1401-1426M5220 (277) GCGAAAATAGCATAAAAACAACTGTA 1447-1472M5221 (278) AGAAACATATGGTGTTAGCTTTATGG 1487-1512

Table 15. Oligonucleotide Sequences That Can Hybrid with HPV 53 and Corresponding HPV 53 Gene Locations

SEQ ID NO: 5 '-> 3' position consecutive to HPV 53 M5301 (279) TCCGCAACCACACAGTCTAT 6681-6700M5302 (280) CCGCAACCACACAGT 6682-6696M5303 (281) CCGCAACCACACAGTCTATG 6682-6701M5304 (282) CACAGTCTATGTCTACATATAA 6691-6712M5305 (283) CTACATATAATTCAAAGCAA 6703-6722M5306 (284) TAAAATATCCCTGTCTGCTGAGG 6782-6804M5307 (285) TTCTACCTTACTGGAAGAC 6830-6848M5308 (286) TTTGTCGCCTCCTGTTGCCACTAGCT 6860-6885M5309 (287) ATACAGATATGTGAAAAGTGCA 6935-6953M5310 (288) GGATCAGCCCCCTCCTGAA 6935-6953

Table 16. Oligonucleotide Sequences That Can Hybrid with HPV 54 and Corresponding HPV 54 Gene Locations

Position consecutive to SEQ ID NO: 5 '-> 3' HPV 54 M5401 (289) TACAGCATCCACGCA 6633-6647M5402 (290) CAGCATCCACGCAGGATAGC 6635-6654M5403 (291) ACGCAGGATAGCTTTAATAA 6643-6662M5404 (292) CACGCAGGATAGCTTTAATA 6642-6661M5405 (293) ATAGCTTTAATAATTCTGAC 6650-6669M5406 (294) TACCATAACCCTTACAGCAGATG 6729-6751M5407 (295) TCCCACTATTCTAGAGGAC 6777-6795M5408 (296) TATAACCCCCCCAGCTACAAGTAGTT 6807-6832M5409 (297) ATATAGGTTTGTACAGTCACAG 6843-6864M5410 (298) GAATAATGCCCCTGCAAAGGAA 6882-6903M5411 (299) GCTGCAGGCAGATGTAGAGGCAG 1043-1065M5412 (300) CGTATGTAAGTCCTGTTGCAAACAGCGA 1099-1128ACM5413 (301) CTGTGTAGAAAAGGACCTAA 1130-1149M5414 (302) TATATCCCTAGGACGGCGGTCAGCCA 1166-1191M5415 ( 303) GGTAAATACCGAGGGGACAGATGAAA 1265-1290M5416 (304) GAAACAACTACAGATAGCCTAGGAAG 1323-1348M5417 (305) CGTGTAGCATTGTTTGGTATGTTTAA 1386-1411M5418 (306) TATGGATTAAGTTTTATGGACC 1419-1440

Table 17. Oligonucleotide Sequences That Can Hybrid with HPV 56 and Corresponding HPV 56 Gene Locations

SEQ ID NO: 5 '-> 3' position consecutive to HPV 56 M5601 (307) CTGCTACAGAACAGT 6630-6644M5602 (308) GCTACAGAACAGTTAAGTAA 6632-6651M5603 (309) CAGAACAGTTAAGTAAATAT 6636-6655M5604 (310) GAACAGTTAAGTAAATATGATGC 6638-6660M5605 (311) GTAAATATGATG6ACGAAGAT6A6AAAGA6 (314) GTTATCCCCGCCAGTGGCCACCAGCC 6805-5830M5609 (315) ATATAGATATGTTAGAAGCACA 6841-6862M5610 (316) GGAACAGCCACCAACAGAA 6880-6898

Table 18. Oligonucleotide Sequences That Can Hybrid with HPV 58 and Corresponding HPV 58 Gene Locations

Position consecutive to SEQ ID NO: 5 '-> 3' HPV 58 M5801 (317) ATGCACTGAAGTAACTAAGG 6674-6693M5802 (318) CACTGAAGTAACTAAGGAAG 6677-6696M5803 (319) TGAAGTAACTAAGGA 6680-6694M5804 (320) GAAGTAACTAAGGAAGGTAC 6681-6700M5805 (321) CTAAGGAAGGATACATAAAAAAAATA (324) TTCCAATATTTTGGAGGAC 6824-6842M5809 (325) TTTAACACCTCCTCCGTCTGCCAGTT 6854-6879M5810 (326) ATATAGATTTGTTACCTCCCAG 6890-6911M5811 (327) AACAGCACCCCCTAAAGAA 6929-6947M5812 (328) AGGGGTGGACGATATAAATGCTG 1104-1126M5813 (329) GCATGCTCAGAAAGTGCTGTAGA 1153-1175M5814 (330) GCAAATGTGTGTGTATCGTGGAAATATA 1195-1224AAM5815 ( 331) AAATTATTGAGCTAGAAGACAG 1253-1274M5816 (332) GCACACCAGGTAGAAAGCCAA 1312-1332M5817 (333) GGCTAGTTCAGATGTAAGCAGTGAAA 1377-1402M5818 (334) GTAATATTCTACATAACAGTAA 1445-1466M5819 (335) GCAACGCTATA336

Table 19. Oligonucleotide Sequences That Can Hybrid with HPV 59 and Corresponding HPV 59 Gene Locations

SEQ ID NO: 5 '-> 3' position consecutive to HPV 18 M5901 (337) TTCTACTACTTCTTC 6643-6657M5902 (338) ACTACTTCTTCTATTCCTAA 6647-6666M5903 (339) ACTTCTTCTATTCCTAATGT 6650-6669M5904 (340) TCTTCTATTCCTAATGTATACAC 6653-6675M5905 (341) ATGTATACACACCTACCAGT 6666-6685M5906 (342) TAAAATAACATTAACTACAGAGG 6745-6767M5907 (343) TACCACTATTTTGGAGGAT 6793-6811M5908 (344) TGTTACACCACCTCCTACTGCTAGTT 6823-6848M5909 (345) ATACCGTTTTGTTCAATCTGCT 6859-6880M5910 (346) GGACACCGCACCGCCAGTT 6898-6916M5911 (347) TTATGACAAACTAAAG 6928-6943M5912 (348) AGCCCAAAGGGATGCACGGGAAA 1093-1115M5913 (349) GCAGTATAGAAAACAGTAGTGAGAAAGC 1143-1172GGM5914 (350) CCATTACAAGAAATATCAGTAAATG 1196-1220M5915 ( 351) GGTTAATAACAGTGCCAGACAGCG 1245-1268M5916 (352) GGTAACCGTGGAGAATACTGGAAATG 1306-1331M5917 (353) CTGTAGCGACAGCAGTAACATGGATG 1372-1397M5918 (354) CCCACTAATCAATTGTTACAGTTA 1421-1444GG20TGT-15355

Table 20. Oligonucleotide sequences that can hybridize with HPV 61 and corresponding HPV 61 gene positions

SEQ ID NO: 5 '-> 3' position consecutive to HPV 18 M6101 (356) CTGCTACATCCCCCC 6803-6817M6102 (357) ACATCCCCCCCTGTATCTGA 6808-6827M6103 (358) CATCCCCCCCTGTATCTGAA 6809-6828M6104 (359) CCCCTGTATCTGAATATAAAGC 6815-6836M6105 (360) CTGAATACAA (363) TGTGGTACCACCACCCTCTACCAGTT 6981-7006M6109 (364) ATATAGGTTTTTGCAGTCCAGA 7017-7038M6110 (365) GGGTGCTGCTGCCCCGCCGCCC 7056-7077M6111 (366) CTATGCCAAGTTATCC 7089-7104M6112 (367) TGCACAGGATGACGCTGCAACGG 1038-1060M6113 (368) CCTTGGTGGACAGTGAATTAAGTCCC 1115-1140M6114 (369) GGACAGGACAGGGCTAGGAGAAGGCTG 1168-1197TTTM6115 ( 370) TGTTTGAGCAAGATAGTGGC 1193-1212M6116 (371) GGATGCGCAACATGAGGGGGGGGGGG 1263-1288M6117 (372) GGCCGAGGCCACAGGTAACCAGGAAA 1335-1360M6118 (373) GGCAGACATATTAGAGGTGT (1380-1405M61AAGCTAGTAGCTAGTAGAGCTAGTAGAGAGCAGTCAGGG

Table 21. Oligonucleotide Sequences That Can Hybrid with HPV 62 and Corresponding HPV 62 Gene Locations

SEQ ID NO: 5 '-> 3' position consecutive to HPV 18 M6201 (376) CCGCCTCCACTGCTG 92-106M6202 (377) GCCTCCACTGCTGCAGCAGA 94-113M6203 (378) CTGCTGCAGCAGAATACACG 101-120M6204 (379) GCAGAATACACGGCTACCAA 109-128M6205 (380) CAGAATACACGGCTACCAAC 110-129M6206 (381) CAAAATACAGTTAACCCCCGAAA 189-211M6207 (382) CAAGGACCTTTTGGATGAC 237-255M6208 (383) GGTTTTACCTCCCCCTTCCACTAGTT 267-292M6209 (384) ATATCACTATTTCGAGTCTCGG 303-324M6210 (385) GGGGCTGCCTACCCGTCCC 342-360M6211 (386) GTATGCGCAAATGACA 372-387

Table 22. Oligonucleotide sequences that can hybridize with HPV 66 and corresponding HPV 66 gene positions

SEQ ID NO: 5 '-> 3' position consecutive to HPV 18 M6601 (387) CAGCTAAAAGCACAT 6680-6694M6602 (388) CAGCTAAAAGCACATTAACT 6680-6699M6603 (389) CTAAAAGCACATTAACTAAA 6683-6702M6604 (390) TTAACTAAATATGATGCCCG 6694-6713M6605 (391) CTAAATATGATGCCCGTGAA 6698-6717M6606 (392) TAAAATAACCTTAACTGCAGAAG 6777-6799M6607 (393) TAATACTTTATTAGACGAT 6825-6843M6608 (394) CTTATCCCCACCAGTTGCAACTAGCT 6855-6880M6609 (395) ATATAGGTATATTAAAAGCACA 6891-6912M6610 (396) GGAACAGCCCCCTGCAGAA 6930-6948M6611 (397) CCTGGCTAAATATAAG 6960-6975M6612 (398) AGCACATGCAGATGCACAGACG 1116-1137M6613 (399) GGTAGTCCCTTAAGTGATATTAGTAA 1165-1190M6614 (400) GCAAACTGTGTACCGAGAGGAAGTAA 1194-1219M6615 ( 401) AAGGCTAATATTATCAGAAGACAGCGGG 1224-1253TAM6616 (402) GAAACATCACAACAGGTAGAATACG 1276-1300M6617 (403) GAGCTCACAAAATGGAGGCTCGCAAA 1320-1345M6618 (404) ATCAAATATGGATATAGATACAAATA 1371-1396M6619 (405) CCAATTGCAGGAACTATTTAAAAGTA 1413-1438M6620 (406) CAAGGAAGATTACATTTTAAATTTAA 1447-1472M6621 (407) AGAAGTGTATGGAGTGCCAT 1473-1492

Table 23. Oligonucleotide Sequences That Can Hybrid with HPV 67 and Corresponding HPV 67 Gene Locations

SEQ ID NO: 5 '-> 3' position consecutive to HPV 18 M6701 (408) CTGAGGAAAAATCAG 6655-6669M6702 (409) GAGGAAAAATCAGAGGCTAC 6657-6676M6703 (410) ATCAGAGGCTACATACAAAAATG 6665-6687M6704 (411) AGGAAAAATCAGAGGCTACA 6658-6677M6705 (412) CTACATACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGA (415) CCTTACACCACCTCCTTCAGGTAATT 6830-6855M6709 (416) ATATAGATTTGTTACCTCGCAG 6866-6887M6710 (417) AACATCCCCTCCAACAGCA 6905-6923M6711 (418) TCTTAAAAAGTACAGT 6935-6950M6712 (419) AGAGGAGGATGACCTAAACGCTG 1096-1118M6713 (420) CAGGCATGTGGTGGTAATAGTAATGG 1151-1176M6714 (421) GCCGCAAAACGCAGAGCATACGACATAG 1199-1228AAM6715 ( 422) GGCAAAATGGCGATATGCAGTGCAGT 1287-1312M6715 (423) GCAAGTAGTACGGGAAACAGTGTAGA 1331-1356M6716 (424) GTCAGGAACAAAGCATGCCATTGCAA 1380-1405M6717 (425) GCATGTAAATAATATAAAGGCAACG 1423-1447M67GA67AC67AT

Table 24. Oligonucleotide sequences capable of hybridizing with HPV 68 and corresponding HPV 68 gene positions

Position contiguous to SEQ ID NO: 5 '-> 3'HPV 68 M6801 (427) CTACTACTGAATCAG 2653-2667M6802 (428) TGAATCAGCTGTACCAAATA 2660-2679M6803 (429) GAATCAGCTGTACCAAATAT 2661-2680M6804 (430) CAGCTGTACCAAATATTTATGA 2665-2686M6805 (431) ATATTCT677AATCTATACTATAATTAAAATTTAAAATTAAAATTAAAATAT (434) TGTTGCCCCTCCACCATCTGCTAGTC 2834-2859M6809 (435) ATACCGCTATCTGCAATCAGCA 2870-2891M6810 (436) AGACGCCCCTGCACCTACT 2909-2927M6811 (437) ATATGATGGCTTAAAC 2939-2954M6812 (438) GGCCCAAAGGGATGCACAAACAG 4990-5012M6813 (439) GCCCTTTAGCAAAGTCGCCATTACAG 5055-5080M6814 (440) GGTAACTGTAGCAACTAATA 5115-5134M6815 ( 441) GGAAAATGGCGACAGCATACGGGAGGA 5163-5192CTGM6816 (442) GACAGTGCTATAGATAGTGAAAACCA 5203-5228M6817 (443) CCTACTACGCAACTAAAAGTATTA 5242-5265M6818 (444) AAGCTGCAATGTTAACAGAATTTAAA 5285-5310

Table 25. Oligonucleotide Sequences That Can Hybrid with HPV 69 and Corresponding HPV 69 Gene Locations

SEQ ID NO: 5 '-> 3' position consecutive to HPV 69 M6901 (445) TATTAGTACTGTATCTGCAC 6572-6591M6902 (446) CTGTATCTGCACAAT 6580-6594M6903 (447) CTGTATCTGCACAATCTGCA 6580-6599M6904 (448) TGCACAATCTGCATCTGCCA 6587-6606M6905 (449) CAATCTGCATCTGCCACTTTTA 6591-6612M6906 (450) CCACTTTTAAACCATCAGAT 6604-6623M6907 (451) TAAAATTACTCTTACCACTGATG 6683-6705M6908 (452) TTCTACTATTTTGGAAAAT 6731-6749M6909 (453) CCTTACCTTGCCTCCTACTGCTAGTT 6761-6786M6910 (454) ATATAGGTTTATTAAAAATTCA 6797-6818M6911 (455) CGATGCCCCTGCACAGCCC 6836-6854M6912 (456) AACACAAGCAAATAAGAAGGCAG 1101-1123M6913 (457) GAACAGCCCGTTGCAAGACATAACAA 1158-1183M6914 (458) CAGACGAAGTAAACAATTTACAGGC 1208-1232M6915 ( 459) GGAGAGCAGTGGACAGTGTTCCGGACAG 1238-1267CGM6916 (460) GGTAGATAAACACAATGAACAAAATG 1308-1333M6917 (461) TCAAGTGGATCTGTATCAGACA 1360-1381M6918 (462) GCACAGGCAAGTAGTGTAGA-1AAMGATGAATAGATATAGTATAATAGTAGTATAATAGTAGTAATAGTAATAGTAATAGTAGTAATAGTAAGTAATAGTAAGTAGTAATAGTAAGTAATAGATAGTAGTAATAGTAGATAGTAATAGGA

Table 26. Oligonucleotide Sequences That Can Hybrid with HPV 6 and Corresponding HPV 6 Gene Locations

SEQ ID NO: 5 '-> 3' position consecutive to HPV 6 M0601 (465) CATCCGTAACTACATCTTCC 6814-6833M0602 (466) ATCCGTAACTACATCTTCCA 6815-6834M0603 (467) CTACATCTTCCACATACACCAA 6823-6844M0604 (468) CATCTTCCACATACACCAAT 6826-6845M0605 (469) ATCTC827AC846CAACACCA (472) TCCCTCTGTTTTGGAAGAC 6959-6977M0609 (473) GTTATCGCCTCCCCCAAATGGTACAT 6989-7014M0610 (474) CTATAGGTATGTGCAGTCACAG 7025-7046M0611 (475) GCCCACTCCTGAAAAGGAA 7064-7082M0612 (476) CTATAAGAACCTTAGT 7094-7109M0613 (477) GGCGGACACCCATTATGCGACTG 1045-1067M0614 (478) GTTAGTCCTATAAACACTATAGCCGA 1106-1131M0615 ( 479) CCACGATTGGACGCCATTAAACTTACAA 1154-1183GAM0616 (480) GGCTGTTTCAAACCAGGGAACTAACG 1206-1231M0617 (481) GGTAGAGAAACATGGCGTACCGGAAA 1279-1304M0618 (482) GGACACAGGAAGGGACATAGAGGGGG 1327-1352M0619 (483) CACAAACAGTGTACGGGAGCATGCAG 1378-1403M0620 (484) GCTAAAATGTAAAGATTTACGGGCAG 1426-1451M0621 (485) GCTTTGGGCTGTCTTTTATAGATTTA 1476-1501

Table 27. Oligonucleotide Sequences That Can Hybrid with HPV 70 and Corresponding HPV 70 Gene Locations

SEQ ID NO: 5 '-> 3' position consecutive to HPV 70 M7001 (486) TGTCTGCCTGCACCGAAACG 6614-6633M7002 (487) CTGCACCGAAACGGC 6621-6635M7003 (488) GAAACGGCCATACCTGCTGT 6628-6647M7004 (489) CGAAACGGCCATACCTGCTG 6627-6646M7005 (490) CGGCCATACCTGCTGTATATAG 6632-6653M7006 (491) CTGTATATAGCCCTACAAAG 6644-6663M7007 (492) TACTATCACATTAACTGCTGACG 6723-6745M7008 (493) TCCTGCAATTTTGGACAAT 6771-6789M7009 (494) AGTTACCCCTCCACCATCTGCAAGCT 6801-6826M7010 (495) GTATAGGTATTTACAATCAGCA 6837-6858M7011 (496) GGATGCTCCTACACCTGAA 6876-6894M7012 (497) CTATGACGATTTAAAA 6906-6921M7013 (498) GGCCCAAAGGGATGCACAATCAG 1149-1171M7014 (499) GCAATCTAAATAAAAGTCCTTGT 1202-1224M7015 ( 500) GTACATAGGGAACAAAGGGTAACAC 1240-1264M7016 (501) GGTAAACATATGCAATAAACAGG 1278-1300M7017 (502) ACAAACGTGTATTCAGTACCAGACAGCG 1306-1335GCM7018 (503) GTAGTAAATAATACAAATGGGGAAGA 1378-1403M7019 (504) GGAGTGCAGTAGTGTAGACAGTGCTA 1446-1471M7020 (505) TCCACAGTCACCTACTGCACAGCTAA 1491-1516M7021 (506) GCTAATAACCAAAAAGCCATACTAC 1531-1555M7022 (507 ) CACACATATGGATTAGCATTTAACGA 157 0-1595

Table 28. Oligonucleotide Sequences That Can Hybrid with HPV 72 and Corresponding HPV 72 Gene Locations

SEQ ID NO: 5 '-> 3' position consecutive to HPV 72 M7201 (508) ATCTGTTGGTTTAATGAGCT 6759-6778M7202 (509) TTTGTGACAGTTGTAGATAC 6780-6799M7203 (510) CTGCCACAGCGTCCT 6829-6843M7204 (511) ACAGCGTCCTCTGTATCAGA 6834-6853M7205 (512) CCACAGCGTCCTCTGTATCA 6832-6851M7206 (513) AGCGTCCTCTGTATCAGAATAT 6836-6857M7207 (514) CAGAATATACAGCTTCTAAT 6850-6869M7208 (515) TAAAATTCACTTAACTCCTGAAA 6929-6951M7209 (516) TAAGGCCTTATTGGATGAC 6977-6995M7210 (517) TGTGGTGCCTCCTCCTTCTACCAGTT 7007-7032M7211 (518) CTATAGGTTTTTGCAGTCTCGT 7043-7064M7212 (519) GGGCTTCTCGCTCTCC

Table 29. Oligonucleotide Sequences That Can Hybrid with HPV 74 and Corresponding HPV 74 Gene Locations

SEQ ID NO: 5 '-> 3' position consecutive to HPV 74 M7401 (521) CCTACCTCACAATCG 1686-1700M7402 (522) CTCACAATCGCCTTCTGCTA 1691-1710M7403 (523) ACCTCACAATCGCCTTCTGC 1689-1708M7404 (524) CAATCGCCTTCTGCTACATATA 1695-1716M7405 (525) ACAATCGCCTTCTGCTACATAT 1694-1715M7406 (526) CTACATATAATAGTTCAGAC 1708-1727M7407 (527) TAGTATTAAGTTAACTGCTGAGG 1787-1809M7408 (528) TCCTACAGTTTTAGAAGAG 1835-1853M7409 (529) GCTAACGCCTCCCCCCAATGGTACTT 1865-1890M7410 (530) CTACAGATATGTGCAGTCCCAG 1901-1922M7411 (531) ACCTACGCCTGATAAAGCA 1940-1958M7412 (532) CTATGCAA1985TAAGT

Table 30. Oligonucleotide sequences that can hybridize with HPV 82 and corresponding HPV 82 gene positions

SEQ ID NO: 5 '-> 3' position consecutive to HPV 82 M8201 (533) TGCTGTTACTCCATC 6608-6622M8202 (534) TGCTGTTACTCCATCTGTTG 6608-6627M8203 (535) ACTCCATCTGTTGCACAAAC 6615-6634M8204 (536) AAACATTTACTCCAGCAAAC 6631-6650M8205 (537) TAAAATCACTTTAACTACTGAAA 6710-6732M8206 (538) TTCTACAATTTTAGAACAG 6758-6776M8207 (539) ATTAACATTGCCCCCCTCCGCTAGTT 6788-6813M8208 (540) CTATCGATTTGTAAAAAATGCA 6824-6845M8209 (541) GGACAGTCCTCCACAGGCT 6863-6881M8210 (542) AACACAGGCACACAAAGAGGCTG 1094-1116M8211 (543) GCAGCCCATTAAAAGACATTACAAA 1156-1180M8212 (544) GTCAGCAACAACCAAAACAGGCAAACCT 1201-1230 TCM8213 (545) GGAGATTACTGGACAGTTATCCGGACA 1243-1269M8214 (546) CCTTACAGGTAGATGGGCAAAATGAC 1309-1334M8215 (547) GAGCAGCGACAGAAGTACAGAGATAG 1367-1392M8216 (548) GCTACCAATGTAGGACTAAACAGTA 1413-1437M8217 (549) GTAGCAATGCAAAAGCAATGTTTATG 1456-1481M8218 (550) GGTGTTAGTTATAATGAGTTGGTAAG 1503-1528

Table 31. Oligonucleotide Sequences That Can Hybrid with HPV CP8061 and Corresponding HPV CP8061 Gene Locations

Position consecutive to SEQ ID NO: 5 '-> 3' HPV CP8061 M806101 (551) TCTGTGCTACCAAAACTGTT 86-105M806102 (552) CTACCAAAACTGTTG 92-106M806103 (553) ACCAAAACTGTTGAGTCTAC 94-113M806104 (554) AACTGTTGAGTCTACATATAAA 99-120M806105 (555) GTTGAGTCTACATATAAAGC 103-122M806ATA (AG) TTATTATAA TACTAA-TTA-TAT-TTA-TTA-TTA (558) TGCTACATTACTGGAGGAC 237-255M806109 (559) GTTCTTACCACCTCCTACTG 267-286M806 110 (560) CTACCGCTTTTTACAGTCTCAG 303-324M806111 (561) AAACAGTCCTCCTCCTGCAGAA 342-363M806112 (562) CTATGCAGATCTTACA

Table 32. Oligonucleotide Sequences That Can Hybrid with HPV CP8304 and Corresponding HPV CP8304 Gene Locations

SEQ ID NO: 5 '-> 3' position consecutive to HPV CP8304 M830401 (563) CAGCTACATCTGCTG 92-106M830402 (564) GCTACATCTGCTGCTGCAGA 94-113M830403 (565) ACATCTGCTGCTGCAGAATACA 97-118M830404 (566) TGCTGCAGAATACAAGGCCT 105-124M830405 (567) GCTGCAGACA-CACACAGAGAGA 211M830408 (570) CAAGGCACTGTTGGATGAT 237-255M830409 (571) TGTGTTGCCACCTCCTTCCACCAGTT 267-292M830410 (572) ATATCGCTTTTTACAGTCTCGG 303-324M830411 (573) GGGTGCTGCTGCCCCTGCGCCC 342-363M830

Table 33. Oligonucleotide sequences that can hybridize with HPV LIAE5 and corresponding HPV LIAE5 gene positions

Position contiguous to SEQ ID NO: 5 '-> 3'HPV LIAE5 MAE501 (575) ATCTACTGCAACTACTAATC 69-88MAE502 (576) CTGCAACTACTAATC 74-88MAE503 (577) CTGCAACTACTAATCCAGTT 74-93MAE504 (578) ACTACTAATCCAGTTCCATCTA 79-100MAE505 (579) CTAATCCAGTTCCATCTATA 83-102MAETATTATACTACTATTATACTA506 (582) TCCTACTATTTTAGATAGT 225-243MAE509 (583) TGTTAGTCCTCCCCCATCTGCTAGCT 255-280MAE510 (584) ATATAGGTTTTTACAGTCATCT 291-312MAE511 (585) GGATGTGGTTGTTCCACAA 330-348

Table 34. Oligonucleotide sequences that can hybridize with HPV MM4 and corresponding HPV MM4 gene positions

Position consecutive to SEQ ID NO: 5 '-> 3' HPV MM4 MM401 (586) CTGCTGTTACTCAATCTGTT 92-111MM402 (587) TGCTGTTACTCAATC 93-107MM403 (588) GTTACTCAATCTGTTGCACA 97-116MM404 (589) TGCACAAACATTTACTCCAG 111-130MM405 (590) TTACTCAATCT1CACACAACACAC-ACACACACACACACACACACACACACCIACT ACC (593) TTCTACAATTTTAGAACAG 243-261MM409 (594) ATTAACCTTGCCCCCCTCAGCTAGTT 273-298MM410 (595) CTATCGATTTGTAAAAAATGCA 309-330MM411 (596) GGACAGTCCTCCACAGGCT 348-366

Table 35. Oligonucleotide sequences that can hybridize with HPV MM7 and corresponding HPV MM7 gene positions

Position consecutive to SEQ ID NO: 5 '-> 3' HPV MM4 MM701 (597) TGCTGCTACACAGGC 93-107MM702 (598) GCTGCTACACAGGCTAATGA 94-113MM703 (599) TGCTACACAGGCTAATGAAT 96-115MM704 (600) CTACACAGGCTAATGAATACAC 98-119MM705 (601) ATGAATACAC601ACGATCACATATCACATAAC601 (604) CGTGTTACCACCTCCTTCCACCAGCC 267-292MM709 (605) CTATCGCTATCTGCAGTCCCGT 303-324MM710 (606) GGGTCCTTCCGCCCCTGCCCCT 342-363MM711 (607) TTATGATGGCCTTGTA 375-390

Table 36. Oligonucleotide sequences that can hybridize with HPV MM8 and corresponding HPV MM8 gene positions

Position consecutive to SEQ ID NO: 5 '-> 3' HPV MM8 MM801 (608) TGCTACCAACACCGA 93-107MM802 (609) CTACCAACACCGAATCAGAA 95-114MM803 (610) CCAACACCGAATCAGAATATAA 98-119MM804 (611) CAGAATATAAACCTACCAAT 110-129MM805 (612) TAAGGTCCGTCTGACTCCAGATT (189-211MMGACC237 (615) CTATAGGTACTTGCAGTCTCGC 303-324MM809 (616) GGGGGCCGCCGCCGCCAAGCCT 342-363MM810 (617) TTATGCTGGCATGTCC 375-390

Next, a method of fixing each nucleotide probe to carrier 11 (nylon membrane) will be described.

1. The 3 'end of the oligonucleotide probe is the addition of a terminal transfer enzyme to the nucleotide. The 3' end of the oligonucleotide probe is composed of an oligo nucleic acid of the 3 'end amino formula Τ Τ Τ Τ Τ Τ Τ Τ Τ Τ Τ Τ Τ Τ Τ Τ oligo nucleic acid.

(1.1) The following articles are mixed uniformly.

Add up to.

(1.2) The mixture is kept at a temperature of 37 ° C. and reacted for 15 to 60 minutes.

(1.3) 10 μl of 0.2 M EDTA (pH 8.0) is added to the mixture to stop the reaction.

2. Oligonucleotide nucleotide probes of 3′-strength increased nucleotides are imprinted on positive carrier 11 (nylon membrane).

(2.1) Each oligo nucleotide probe of the 3'-strength increase nucleotide is imprinted on the positive carrier 11 (nylon membrane) with a pin head 400 µm in diameter. The distance between dots is 1200 micrometers.

(2.2) The carrier 11 (nylon membrane) is irradiated with ultraviolet rays.

(2.3) Detector 10 for identification and differentiation of HPV subtypes contained in the sample at the same time is stored in a dry container for use

10X NEBuffer 4 5 μl 2.5 mM CoCl ₂ 5 μl Oligonucleotide probe 5 to 300 pmol 10-300 mMMdATP, dCTP, dTTP or dGTP 1 μl Terminal transfer enzyme (20U / ul) (NEWEnglish BioLabs, M0252S) 0.5 to 5 μl M, Q, H ₂ O 50 μl

In Fig. 2, oligonucleotide test piece 20 is a second example for simultaneously identifying and discriminating a human papilloma pathogen (HPV) subtype contained in a specimen. The carrier 21 is a glass plate, on which a dot array 22 is fixed. Each dot of the dot array 22 is an oligonucleotide (15 to 26 nucleotides) probe pin, and each oligo nucleotide probe pin may hybridize with deoxyribonucleic acid (DNA) of HPV subtype.

Each oligonucleotide sequence of the carrier 21 is selected with reference to Tables 1-36. For example, in order to hybridize HPV subtype II (HPV 11), the base sequence of the single oligonucleotide probe pin on the carrier 21 may employ the one base sequence of Nos. 1 to 12 (see Table 1).

Next, a method of fixing each nucleotide probe to the carrier 21 (glass plate) will be described.

1.Surface Treatment of Carrier 21 (Glass Plate)

(1.1) Clean the carrier 21 (select a microscope carrier piece from Super Frost, Menzel-Glaser CO. Germany) with an fluorescence-free soft cleaner.

(1.2) The washed carrier 21 is soaked in 10% NaOH overnight.

(1.3) Carrier 21 is shaken for 2 minutes in double-distilled water, 1% brine solution and metal alcohol in order, then placed in an oven and dried.

(1.4) Carrier 21 is immersed in 1% 3-aminopropyltrimethoxysilane in a silanization solution (95% aqueous acetone) and subjected to room temperature reaction for about 2 minutes.

(1.5) The carrier 21 is washed 10 times with acetone (5 minutes each time). Transfer to an oven at 110 ° C. and dry for 45 minutes.

(1.6) After completion of drying, the carrier 21 is immersed in a solution of 0.2% 1,4-phenylene diisothiocyanate (the solvent is 10% pyridine in dimethylformamide) to allow room temperature reaction for about 2 hours. Do it.

(1.7) Finally, the carrier 21 is washed with methyl alcohol and acetone and dried as it is.

(1.8) After completion of surface treatment, the carrier 21 is placed in a vacuum dryer and stored for use.

2. Fix the oligonucleotide probe pin onto carrier 21 (glass plate).

(2.1) 3 'short amino modifications Τ Τ Τ Τ Τ Τ Τ Τ Τ Τ Τ Τ Τ Τ Τ Τ-Oligo nucleic acid sequence is imprinted on the carrier 21 with a pen head 400 mm in diameter. The distance between each dot is 1200 micrometers.

(2.2) The carrier 21 is immersed in 1% ammonia water for about 2 minutes, washed again with secondary water, and then dried by wind at room temperature.

(2.3) Detector 20 for identification and differentiation of HPV subtypes contained in the sample at the same time is stored in dry and provided for use

The method of detecting and discriminating the presence of HPV in a sample is described.

1. Sample processing

(1.1) If a blush or cotton swab is placed in the pipe, first shake it for several seconds to drop the cells, then extract the blush or cotton swab at 20 ℃ and centrifuge at 1500rpm for 5 minutes.

(1.2) After washing the cells dropped in 10mM Tris (pH 8.5) buffer, lysed in 8mM NaOH, the cells are blown into 1.5ml microcentrifuge tube.

(1.3) Sample DNA obtained by adding an appropriate amount of PreTaq (1U / μl) solution based on the volume of the cell precipitate, reacting at 94 ° C for 1 hour, and then at 20 ° C for 5 minutes at 13,500 rpm. Store at -20 ° C.

2. Polymerase Chain Reaction (PCR)

(2.1) The glycerin aldehyde phosphate dehydrogenase gene (Glutaldehyde-3-phosphodehydrogenase gene) is a chain reaction in the internal control (polymerization).

(2.1.1) Add the following substances on the basis of the following list.

Test Stock Extraction amount Final concentration Sterile h ₂ o 2.6 10X Taq Buffer 0.5 1X Taq Buffer dNTP 2.5mM 0.4 200 μM Template One GAP241-5 ¹⁾ primer 10 pmol / μl 0.2 0.4 pmol / μl GAP241-3 ²⁾ primer 10 pmol / μl 0.2 0.4 pmol / μl ProTaq (PROTECH) 5U / μl 0.1 0.1U / μl Total volume (μl) 5

1) Gap 21-5: CCACCAACTGCTTAGCACCCC

2) Gap 21-3: TGCAGCGTACTCCCCACATCA

3) Finally, an appropriate amount of mineral oil is added to prevent evaporation.

(2.1.2) Proceed with the PCR reaction based on the following program.

Program 1 Program 2 Program 3 94 ° C, 15 seconds 94 ℃, 3 minutes 57 ° C, 1 minute 72 ° C., 5 minutes 72 ° C., 30 seconds 45 cycles

(2.1.3) PCR products are analyzed at 2.5% agarose / EtBr (0.5 × TBE).

(2.2) The first polymerase of the sample DNA undergoes a chain reaction.

(2.2.1) Add the following substances based on the table below.

Test Stock Extraction amount Final concentration Sterile h ₂ o 4.7 to 5.7 10X Taq Buffer One 1X Taq Buffer dNTP 2.5mM 0.8 200 mM Template 1.2 BSA 10mg / ml 0.1 0.1 μg / μl primer ^1,2) 10 pmol / μl 0.6 0.6 pmol / μl primer ^1,2) 10 pmol / μl 0.6 0.6 pmol / μl ProTaq (PRO _TECH ) 5U / μl 0.2 0.1U / μl Total volume (μl) 10

1) MY09 / MY11: Manos et al., Cancer Cells 1989, 7, 209-214.

2) E1 301L / E1 847R: Ylitalo et al., J. Clin. Microbiol. 1995, 33, 1822-1828.

3) Finally, an appropriate amount of mineral oil is added to prevent evaporation.

(2.2.2) Proceed with the PCR reaction based on the following program.

Program 1 Program 2 Program 3 94 ° C, 45 seconds 94 ℃, 3 minutes 45 ° C, 1 minute 72 ° C., 5 minutes 72 ° C., 1.5 minutes 45 cycles

(2.2.3) PCR products are analyzed at 2.5% agarose / EtBr (0.5 × TBE).

(2.3) The secondary polymerase reaction is carried out using an internal primer.

(2.3.1) The following substances are added based on the following table.

Test Stock Extraction amount Final concentration Sterile h ₂ o 11.75 10X Taq Buffer 2.5 1X Taq Buffer dNTP 2.5mM 2 200 μM Primary PCR Product 5 BSA 10mg / ml 0.25 0.1 μg / μl primer ¹⁾ 10 pmol / μl 1.5 0.6 pmol / μl primer ^1,2,3) 10 pmol / μl 1.5 0.6 pmol / μl ProTaq (PRO _TECH ) 5U / μl 0.5 0.1U / μl Total volume (μl) 25

1) GP5 + / GP6 +: De Roda Husman et al., J. Gen. Virol. 1995, 76, 1057-1062.

2) E1 350L / E1 547R: Ylitalo et al., J. Clin. Microbiol. 1995, 33, 1822-1828.

3) The biomarker Cy5 fluorescent mark may be added to the 5 'end of the GP6 + and E1 547R primers.

4) Finally, an appropriate amount of mineral oil is added to prevent evaporation.

(2.3.2) PCR reaction is carried out by the following program.

Program 1 Program 2 Program 3 94 ° C, 45 seconds 94 ℃, 3 minutes 45 ° C, 1 minute 72 ° C., 5 minutes 72 ° C., 1.5 minutes 45 cycles

(2.3.3) PCR products are analyzed at 2.5% agarose / EtBr (0.5 × TBE).

3. Detect HPV subtypes using oligonucleotide test piece 10 (carrier 11 is a nylon membrane) provided by the present application.

(3.1) The oligonucleotide test piece 10 is immersed in 2 x SSC solution and operated for 5 minutes.

(3.2) The oligonucleotide test piece 10 is placed in a hybrid buffer containing 50 μg / μl salmon sperm nucleotides and hybridized in advance for about 30 minutes at a temperature of 35 ° C.

(3.3) The polymerase reaction product of the sample containing the biotin mark is uniformly mixed with a hybrid buffer solution containing 50 μg / μl salmon sperm nucleic acid, heated at a temperature of 95 ° C. for about 5 minutes, and the nucleic acid is denatured and placed on ice. Provide for use.

(3.4) After modification, the polymerase chain reaction product is added to the nucleotide dot array on the 10th position of the oligonucleotide test piece, and hybridized at a temperature of 35 ° C. for about 4 hours or overnight.

(3.5) Rinse the oligonucleotide specimen 10 for approximately 10 minutes at a temperature of 35 ° C. using a 2 × SSC / 1% SDS solution.

(3.6) Using the 0.2 × SSC / 0.1% SDS solution, wash the oligonucleotide test piece 10 at a temperature of 35 ° C. for about 10 minutes.

(3.7) Oligonucleotide 10 is treated for about 1 hour in a 0.5% isolation reagent.

(3.8) The oligonucleotide test piece 10 is reacted for about 1 hour by a protein-alkaline phosphatase conjugate (avidin-alkalinephosphatase).

(3.9) The oligonucleotide test piece 10 is washed twice with 1 × PBST (5 minutes each time).

(3.10) Oligonucleotide test piece 10 is washed twice with Tris / NaCl buffer (5 min each time).

(3.11) Oligonucleotide Specimen 10 undergoes color development at room temperature with NBT / BCIP.

(3.12) If each of the color dots on the 10th oligonucleotide test piece are mapped to the hybrid HPV subtype, it can be read what kind of HPV subtype is contained in the sample.

4. Detect HPV subtypes using oligonucleotide test piece 20 (carrier 21 is a glass plate) provided by the present applicant.

(4.1) Purify polymerase chain reaction product containing Cy5 fluorescent mark in PCR Clear Up-M System (Viogene, USA), precipitate polymerase chain reaction product containing Cy5 fluorescent mark with alcohol, and finally The precipitate is vacuum dried to provide for use.

(4.2) The precipitate is added to a 12 µl mixed solution (2 x SSC / 0.1% SDS), dissolved sufficiently, and then centrifuged for 1 minute, followed by boiling water for 2 minutes.

(4.3) After extraction, insert into ice quickly and leave for 5 minutes. After centrifugation for 30 seconds, 10 μl is raised and extracted to the left side of the nucleic acid micro array hybrid sphere, and carefully the one cover glass plate is pulled along the left side to cover the solution on the hybrid sphere to avoid the generation of bubbles. The oligo nucleic acid micro array hybrids are faced down and placed in a Humid Chamber (Sigma, USA) and hybridized for 4 hours or overnight at a temperature of 35 ° C.

(4.4) After completion of the hybridization, the oligonucleotide test piece 20 is placed vertically in a 50 ml sintering cap of the washing solution A (2 × SSC / 1% SDS), and the light oligonucleotide test piece 20 is vibrated to drop the cover glass plate, and then again The nucleotide test piece 20 was placed in a 50 ml centrifuge tube containing the washing solution, and washed for 12 minutes on a planar shaker at a speed of 160 rpm.

(4.5) Subsequently, after the oligonucleotide test piece 20 was vibrated at a temperature of 35 ° C. for 12 minutes in washing solution B (0.2 × SSC / 0.1% SDS), the surface was washed with secondary water and dried as it was (靜置 乾燥).

(4.6) GenePix ^™ 4000 (Axon, USA) scanning was performed on the oligonucleotide test piece 20 after completion of drying, the 635 nm triggered wavelength was selected, and analyzed by GenePix Pro 3.0 (Axon, USA) software.

Fig. 3 (a) in Fig. 3 (a) and (b) is a schematic diagram of the third embodiment. In FIG. 3 (a), oligonucleotide test piece 30 is used to simultaneously identify and discriminate HPV subtypes contained in a sample, of which carrier 34 has an actual length of about 1.44 cm and a width of about 0.96 cm. It is a nylon curtain. The dot array 32 is fixed on the carrier 31 by the above method, and the distance between the dots is about 1.2 mm and the diameter of each dot is about 0.4 mm. Each dot of the dot array 32 is one oligonucleotide (15-30 nucleotides) probe pin, and each oligonucleotide probe pin may hybridize with deoxyribonucleic acid (DNA) of HPV subtype. 3 (b) is a diagram showing the HPV subtype to which each oligonucleotide probe in the dot array 32 corresponds. Each oligonucleotide probe pin base sequence is selected from the base sequences in Tables 1 to 36. Among them, SC (System Control) is a product in which some kind of HPV subtype is chain-reacted by a polymerase for biotin primers, and NC (negative control) is part of a plant gene not related to HPV, and internal control (IC) Is a full-time detection pin for the housekeeping gene Glyceraldehyde-3-Phosphate Dehydrogenase (GAP-DH) (base sequence 5'-gcccagactgtgggtggcag-3 '). This is to monitor and detect whether it is normal.

Positive clones containing the specific HPV subtype partial genes are detected for oligonucleotide test piece 30, respectively, based on the above method, and the results are shown in Figs. (A) (b) and (c). The positive clone containing the specific HPV subtype partial gene undergoes a first polymerase reaction by MY11 / MY09 primer, and analyzes the obtained first polymerase chain reaction product in 2.5% agarose / EtBr. The result of the electrophoresis is shown in Fig. 4 (a). These polymerase chain reaction products are subjected to secondary polymerase reaction by GP5 / GP6 primers, and the obtained secondary polymerase chain reaction products are analyzed in 2.5% agarose / EtBr to show the results of electrophoresis. It shows in 4 (b). These HPV subtype positive clones and their marks are shown in Table 37.

Table 37.

Mark HPV Positive Clone Mark HPV Positive Clone Mark HPV Positive Clone M DNA Marker 7 HPV 33 14 HPV 56 One HPV 6 8 HPV 35 15 HPV 59 2 HPV 11 9 HPV 44 16 HPV 61 3 HPV 16 10 HPV 45 17 HPV 66 4 HPV 18 11 HPV 52 18 HPV 70 5 HPV 26 12 HPV 53 19 HPV CP 8061 6 HPV 31 13 HPV 54 20 HPV L1AE5

Secondary polymerase reaction products of the marks (1) to (20) were detected in 20 oligonucleotide test pieces 30, respectively. The results are shown in Fig. 4C. Using the SC as a reference point and comparing the detection result of FIG. Able to know.

In the present invention, oligonucleotide test piece 30 is applied separately to the discrimination of HPV subtypes in the examinee's specimen, and the results are shown in FIG. Using the SC as a reference point, the detection result of FIG. 5 is compared with FIG. 3 (b). It can be seen that the HPV type 52 (HPV 52) is present in the sample 3 and the HPV type 39 (HPV 39) is present in the sample 4.

Fig. 6 (a) in Fig. 6 (a) and (b) is a schematic diagram of the fourth embodiment. In the figure, oligonucleotide test piece 40 is used to simultaneously identify and discriminate HPV subtypes contained in a sample, wherein carrier 41 is a glass plate. The dot array 42 is fixed on the carrier 41 by the above method. Each dot of the dot array 42 is one oligonucleotide (15-30 nucleotides) probe pin, and each oligo nucleotide probe pin may hybridize with deoxyribonucleic acid (DNA) of the HPV subtype. 6 (b) is a diagram showing the HPV subtype to which each oligonucleotide probe in the dot array 42 corresponds. Each oligo nucleotide probe pin sequence is selected from the sequences in Tables 1, 3 or 14.

Oligonucleotide test piece 40 was stained with SYBR Green II, and scanned using GenePix ^™ 4000 (Axon, USA) to select 635 nm trigger wavelength. The result is shown in Fig. 7A. Positive clones of HPV 11-type and 18-type partial genes were detected by oligonucleotide test piece 40 through the products of the polymerase reaction twice, respectively, and the results are shown in Fig. 7B. In the figure, red fluorescence shows a positive reaction. As a result of comparing the detection result of FIG.

Thus, the "detector and detection method for simultaneously identifying and discriminating multiple human papilloma pathogen subtypes" of the present invention not only overcomes the combination of the prior art but also has industrial applicability.

The technical idea of the present invention is not limited to the above embodiments, and simple changes and modifications made by those skilled in the art also fall within the technical scope of the present invention unless the claims are departed from the appended claims.

The present invention relates to a detector and a detection method used for differentiation of pathogens, and in particular, a detector and a detection method which are easy to discriminate against a plurality of human papilloma pathogens (HPVs) are provided.

<110> KING CAR FOOD INDUSTRIAL CO., LTD. <120> METHOD AND DETECTOR FOR IDENTIFYING SUBTYPES OF HUMAN PAPILLOMA VIRUSES <130> 1 <140> 10-2001-0071849 <141> 2001-11-19 <160> 617 <170> KopatentIn 1.71 <210> 1 <211> 15 <212> DNA <213> Human papillomavirus type 11 <220> <221> -35_signal (222) (1) .. (15) <223> 6799-6813 <400> 1 atctgtgtct aaatc 15 <210> 2 <211> 20 <212> DNA <213> Human papillomavirus type 11 <220> <221> -35_signal (222) (1) .. (20) <223> 6800-6819 <400> 2 tctgtgtcta aatctgctac 20 <210> 3 <211> 26 <212> DNA <213> Human papillomavirus type 11 <220> <221> -35_signal (222) (1) .. (26) <223> 6799-6824 <400> 3 atctgtgtct aaatctgcta cataca 26 <210> 4 <211> 20 <212> DNA <213> Human papillomavirus type 11 <220> <221> -35_signal (222) (1) .. (20) <223> 6796-6815 <400> 4 tgcatctgtg tctaaatctg 20 <210> 5 <211> 20 <212> DNA <213> Human papillomavirus type 11 <220> <221> -35_signal (222) (1) .. (20) <223> 6809-6828 <400> 5 aaatctgcta catacactaa 20 <210> 6 <211> 21 <212> DNA <213> Human papillomavirus type 11 <220> <221> -35_signal (222) (1) .. (21) <223> 6807-6827 <400> 6 ctaaatctgc tacatacact a 21 <210> 7 <211> 20 <212> DNA <213> Human papillomavirus type 11 <220> <221> -35_signal (222) (1) .. (20) <223> 6816-6835 <400> 7 ctacatacac taattcagat 20 <210> 8 <211> 23 <212> DNA <213> Human papillomavirus type 11 <220> <221> -35_signal (222) (1) .. (23) <223> 6895-6917 <400> 8 tagcattaca ttatctgcag aag 23 <210> 9 <211> 19 <212> DNA <213> Human papillomavirus type 11 <220> <221> -35_signal (222) (1) .. (19) <223> 6943-6961 <400> 9 tccttctgtt ttggaggac 19 <210> 10 <211> 26 <212> DNA <213> Human papillomavirus type 11 <220> <221> -35_signal (222) (1) .. (26) <223> 6973-6998 <400> 10 tttatcgcct ccaccaaatg gtacac 26 <210> 11 <211> 25 <212> DNA <213> Human papillomavirus type 11 <220> <221> -35_signal (222) (1) .. (25) <223> 7009-7033 <400> 11 ttatagatat gtacagtcac aggcc 25 <210> 12 <211> 23 <212> DNA <213> Human papillomavirus type 11 <220> <221> -35_signal (222) (1) .. (23) <223> 7048-7070 <400> 12 acccacacct gaaaaagaaa aac 23 <210> 13 <211> 23 <212> DNA <213> Human papillomavirus type 11 <220> <221> -35_signal (222) (1) .. (23) <223> 1044-1066 <400> 13 ggcggatgct cattatgcga ctg 23 <210> 14 <211> 26 <212> DNA <213> Human papillomavirus type 11 <220> <221> -35_signal (222) (1) .. (26) <223> 1101-1126 <400> 14 atatgtaagt cctataagca atgtag 26 <210> 15 <211> 26 <212> DNA <213> Human papillomavirus type 11 <220> <221> -35_signal (222) (1) .. (26) <223> 1127-1152 <400> 15 ctaatgcagt agaaagtgag ataagt 26 <210> 16 <211> 26 <212> DNA <213> Human papillomavirus type 11 <220> <221> -35_signal (222) (1) .. (26) <223> 1154-1179 <400> 16 cacggttaga cgccattaaa cttaca 26 <210> 17 <211> 26 <212> DNA <213> Human papillomavirus type 11 <220> <221> -35_signal (222) (1) .. (26) <223> 1181-1206 <400> 17 cacagccaaa aaaggtaaag cgacgg 26 <210> 18 <211> 26 <212> DNA <213> Human papillomavirus type 11 <220> <221> -35_signal (222) (1) .. (26) <223> 1264-1289 <400> 18 gcaacgcagg tagagaaaca tggcga 26 <210> 19 <211> 30 <212> DNA <213> Human papillomavirus type 11 <220> <221> -35_signal (222) (1) .. (30) <223> 1294-1323 <400> 19 gaaaatgggg gagatggtca ggaaagggac 30 <210> 20 <211> 26 <212> DNA <213> Human papillomavirus type 11 <220> <221> -35_signal (222) (1) .. (26) <223> 1321-1346 <400> 20 gacacaggga gggacataga gggtga 26 <210> 21 <211> 26 <212> DNA <213> Human papillomavirus type 11 <220> <221> -35_signal (222) (1) .. (26) <223> 1356-1381 <400> 21 acatagagag gcggaagcag tagacg 26 <210> 22 <211> 26 <212> DNA <213> Human papillomavirus type 11 <220> <221> -35_signal (222) (1) .. (26) <223> 1382-1407 <400> 22 acagcacccg agagcatgca gacaca 26 <210> 23 <211> 26 <212> DNA <213> Human papillomavirus type 11 <220> <221> -35_signal (222) (1) .. (26) <223> 1408-1433 <400> 23 tcaggaatat tagaattact aaaatg 26 <210> 24 <211> 19 <212> DNA <213> Human papillomavirus type 11 <220> <221> -35_signal (222) (1) .. (19) <223> 1482-1500 <400> 24 gctgtcattt gttgattta 19 <210> 25 <211> 20 <212> DNA <213> Human papillomavirus type 16 <220> <221> -35_signal (222) (1) .. (20) <223> 6659-6678 <400> 25 tatgtcatta tgtgctgcca 20 <210> 26 <211> 20 <212> DNA <213> Human papillomavirus type 16 <220> <221> -35_signal (222) (1) .. (20) <223> 6670-6689 <400> 26 gtgctgccat atctacttca 20 <210> 27 <211> 15 <212> DNA <213> Human papillomavirus type 16 <220> <221> -35_signal (222) (1) .. (15) <223> 6674-6688 <400> 27 tgccatatct acttc 15 <210> 28 <211> 20 <212> DNA <213> Human papillomavirus type 16 <220> <221> -35_signal (222) (1) .. (20) <223> 6678-6698 <400> 28 tatctacttc agaaactaca 20 <210> 29 <211> 22 <212> DNA <213> Human papillomavirus type 16 <220> <221> -35_signal (222) (1) .. (22) <223> 6682-6703 <400> 29 ctacttcaga aactacatat aa 22 <210> 30 <211> 20 <212> DNA <213> Human papillomavirus type 16 <220> <221> -35_signal (222) (1) .. (20) <223> 6700-6719 <400> 30 ataaaaatac taactttaag 20 <210> 31 <211> 23 <212> DNA <213> Human papillomavirus type 16 <220> <221> -35_signal (222) (1) .. (23) <223> 6773-6795 <400> 31 caaaataacc ttaactgcag acg 23 <210> 32 <211> 19 <212> DNA <213> Human papillomavirus type 16 <220> <221> -35_signal (222) (1) .. (19) <223> 6821-6839 <400> 32 ttccactatt ttggaggac 19 <210> 33 <211> 26 <212> DNA <213> Human papillomavirus type 16 <220> <221> -35_signal (222) (1) .. (26) <223> 6851-6876 <400> 33 tctacaacct cccccaggag gcacac 26 <210> 34 <211> 19 <212> DNA <213> Human papillomavirus type 16 <220> <221> -35_signal (222) (1) .. (19) <223> 6887-6905 <400> 34 ttataggttt gtaacccag 19 <210> 35 <211> 19 <212> DNA <213> Human papillomavirus type 16 <220> <221> -35_signal (222) (1) .. (19) <223> 6923-6941 <400> 35 acatacacct ccagcacct 19 <210> 36 <211> 16 <212> DNA <213> Human papillomavirus type 16 <220> <221> -35_signal (222) (1) .. (16) <223> 6956-6971 <400> 36 ccttaaaaaa tacact 16 <210> 37 <211> 23 <212> DNA <213> Human papillomavirus type 16 <220> <221> -35_signal (222) (1) .. (23) <223> 1089-1111 <400> 37 agcaaaacaa catagagatg cag 23 <210> 38 <211> 26 <212> DNA <213> Human papillomavirus type 16 <220> <221> -35_signal (222) (1) .. (26) <223> 1145-1170 <400> 38 acttagtgat attagtggat gtgtag 26 <210> 39 <211> 30 <212> DNA <213> Human papillomavirus type 16 <220> <221> -35_signal (222) (1) .. (30) <223> 1181-1210 <400> 39 tagtcctaga ttaaaagcta tatgtataga 30 <210> 40 <211> 26 <212> DNA <213> Human papillomavirus type 16 <220> <221> -35_signal (222) (1) .. (26) <223> 1209-1234 <400> 40 gaaaaacaaa gtagagctgc aaaaag 26 <210> 41 <211> 26 <212> DNA <213> Human papillomavirus type 16 <220> <221> -35_signal (222) (1) .. (26) <223> 1285-1310 <400> 41 ctcagcagat gttacaggta gaaggg 26 <210> 42 <211> 26 <212> DNA <213> Human papillomavirus type 16 <220> <221> -35_signal (222) (1) .. (26) <223> 1313-1338 <400> 42 ccatgagact gaaacaccat gtagtc 26 <210> 43 <211> 26 <212> DNA <213> Human papillomavirus type 16 <220> <221> -35_signal (222) (1) .. (26) <223> 1356-1381 <400> 43 gggggtggtt gcagtcagta cagtag 26 <210> 44 <211> 26 <212> DNA <213> Human papillomavirus type 16 <220> <221> -35_signal (222) (1) .. (26) <223> 1387-1412 <400> 44 gtgggggaga gggtgttagt gaaaga 26 <210> 45 <211> 26 <212> DNA <213> Human papillomavirus type 16 <220> <221> -35_signal (222) (1) .. (26) <223> 1417-1442 <400> 45 ctatatgcca aacaccactt acaaat 26 <210> 46 <211> 26 <212> DNA <213> Human papillomavirus type 16 <220> <221> -35_signal (222) (1) .. (26) <223> 1502-1527 <400> 46 gttatacggg gtgagttttt cagaat 26 <210> 47 <211> 15 <212> DNA <213> Human papillomavirus type 18 <220> <221> -35_signal (222) (1) .. (15) <223> 6650-6664 <400> 47 ttctacacag tctcc 15 <210> 48 <211> 20 <212> DNA <213> Human papillomavirus type 18 <220> <221> -35_signal (222) (1) .. (20) <223> 6657-6676 <400> 48 cagtctcctg tacctgggca 20 <210> 49 <211> 20 <212> DNA <213> Human papillomavirus type 18 <220> <221> -35_signal (222) (1) .. (20) <223> 6658-6677 <400> 49 agtctcctgt acctgggcaa 20 <210> 50 <211> 23 <212> DNA <213> Human papillomavirus type 18 <220> <221> -35_signal (222) (1) .. (23) <223> 6660-6682 <400> 50 tctcctgtac ctgggcaata tga 23 <210> 51 <211> 20 <212> DNA <213> Human papillomavirus type 18 <220> <221> -35_signal (222) (1) .. (20) <223> 6664-6683 <400> 51 ctgtacctgg gcaatatgat 20 <210> 52 <211> 20 <212> DNA <213> Human papillomavirus type 18 <220> <221> -35_signal (222) (1) .. (20) <223> 6679-6698 <400> 52 atgatgctac caaatttaag 20 <210> 53 <211> 23 <212> DNA <213> Human papillomavirus type 18 <220> <221> -35_signal (222) (1) .. (23) <223> 6752-6774 <400> 53 tactattact ttaactgcag atg 23 <210> 54 <211> 19 <212> DNA <213> Human papillomavirus type 18 <220> <221> -35_signal (222) (1) .. (19) <223> 6800-6818 <400> 54 tagcagtatt ttagaggat 19 <210> 55 <211> 26 <212> DNA <213> Human papillomavirus type 18 <220> <221> -35_signal (222) (1) .. (26) <223> 6830-6855 <400> 55 tgttcccccc cccccaacta ctagtt 26 <210> 56 <211> 22 <212> DNA <213> Human papillomavirus type 18 <220> <221> -35_signal (222) (1) .. (22) <223> 6866-6887 <400> 56 atatcgtttt gtacaatctg tt 22 <210> 57 <211> 19 <212> DNA <213> Human papillomavirus type 18 <220> <221> -35_signal (222) (1) .. (19) <223> 6905-6923 <400> 57 ggatgctgca ccggctgaa 19 <210> 58 <211> 16 <212> DNA <213> Human papillomavirus type 18 <220> <221> -35_signal (222) (1) .. (16) <223> 6935-6950 <400> 58 ctatgataag ttaaag 16 <210> 59 <211> 23 <212> DNA <213> Human papillomavirus type 18 <220> <221> -35_signal (222) (1) .. (23) <223> 1135-1157 <400> 59 ggtccacaat gatgcacaag tgt 23 <210> 60 <211> 26 <212> DNA <213> Human papillomavirus type 18 <220> <221> -35_signal (222) (1) .. (26) <223> 1192-1217 <400> 60 cacagaaaac agtccattag gggagc 26 <210> 61 <211> 26 <212> DNA <213> Human papillomavirus type 18 <220> <221> -35_signal (222) (1) .. (26) <223> 1219-1244 <400> 61 gctggaggtg gatacagagt taagtc 26 <210> 62 <211> 30 <212> DNA <213> Human papillomavirus type 18 <220> <221> -35_signal (222) (1) .. (30) <223> 1271-1300 <400> 62 agtgggcaga aaaaggcaaa aaggcggctg 30 <210> 63 <211> 26 <212> DNA <213> Human papillomavirus type 18 <220> <221> -35_signal (222) (1) .. (26) <223> 1347-1372 <400> 63 cacagattca ggtaactaca aatggc 26 <210> 64 <211> 26 <212> DNA <213> Human papillomavirus type 18 <220> <221> -35_signal (222) (1) .. (26) <223> 1384-1409 <400> 64 caatgtatgt agtggcggca gtacgg 26 <210> 65 <211> 26 <212> DNA <213> Human papillomavirus type 18 <220> <221> -35_signal (222) (1) .. (26) <223> 1418-1443 <400> 65 gacaacgggg gcacagaggg caacaa 26 <210> 66 <211> 26 <212> DNA <213> Human papillomavirus type 18 <220> <221> -35_signal (222) (1) .. (26) <223> 1451-1476 <400> 66 gtagacggta caagtgacaa tagcaa 26 <210> 67 <211> 26 <212> DNA <213> Human papillomavirus type 18 <220> <221> -35_signal (222) (1) .. (26) <223> 1493-1518 <400> 67 ccacaatgta ccatagcaca attaaa 26 <210> 68 <211> 26 <212> DNA <213> Human papillomavirus type 18 <220> <221> -35_signal (222) (1) .. (26) <223> 1573-1598 <400> 68 cacatatggg ctatcattta cagatt 26 <210> 69 <211> 20 <212> DNA <213> Human papillomavirus type 26 <220> <221> -35_signal (222) (1) .. (20) <223> 6619-6638 <400> 69 tagtacatta tctgcagcat 20 <210> 70 <211> 15 <212> DNA <213> Human papillomavirus type 26 <220> <221> -35_signal (222) (1) .. (15) <223> 6625-6639 <400> 70 attatctgca gcatc 15 <210> 71 <211> 20 <212> DNA <213> Human papillomavirus type 26 <220> <221> -35_signal (222) (1) .. (20) <223> 6631-6650 <400> 71 tgcagcatct gcatccactc 20 <210> 72 <211> 24 <212> DNA <213> Human papillomavirus type 26 <220> <221> -35_signal (222) (1) .. (24) <223> 6635-6658 <400> 72 gcatctgcat ccactccatt taaa 24 <210> 73 <211> 20 <212> DNA <213> Human papillomavirus type 26 <220> <221> -35_signal (222) (1) .. (20) <223> 6648-6667 <400> 73 ctccatttaa accatctgat 20 <210> 74 <211> 23 <212> DNA <213> Human papillomavirus type 26 <220> <221> -35_signal (222) (1) .. (23) <223> 6727-6749 <400> 74 taaaataaca cttacaacag atg 23 <210> 75 <211> 19 <212> DNA <213> Human papillomavirus type 26 <220> <221> -35_signal (222) (1) .. (19) <223> 6775-6793 <400> 75 tgcctccata ttggaggat 19 <210> 76 <211> 26 <212> DNA <213> Human papillomavirus type 26 <220> <221> -35_signal (222) (1) .. (26) <223> 6805-6830 <400> 76 actaacctta cctcccactg ctagtt 26 <210> 77 <211> 22 <212> DNA <213> Human papillomavirus type 26 <220> <221> -35_signal (222) (1) .. (22) <223> 6841-6862 <400> 77 ctataggttt attaaaaact ct 22 <210> 78 <211> 19 <212> DNA <213> Human papillomavirus type 26 <220> <221> -35_signal (222) (1) .. (19) <223> 6880-6898 <400> 78 taacgcccct cctgtgcca 19 <210> 79 <211> 23 <212> DNA <213> Human papillomavirus type 26 <220> <221> -35_signal (222) (1) .. (23) <223> 1093-1115 <400> 79 aaaacaggca aatacaaagg cag 23 <210> 80 <211> 26 <212> DNA <213> Human papillomavirus type 26 <220> <221> -35_signal (222) (1) .. (26) <223> 1139-1164 <400> 80 ctaggtagtc agaacagccc gttgca 26 <210> 81 <211> 30 <212> DNA <213> Human papillomavirus type 26 <220> <221> -35_signal (222) (1) .. (30) <223> 1194-1223 <400> 81 gcgacagtca gcagaataca caccaagtaa 30 <210> 82 <211> 26 <212> DNA <213> Human papillomavirus type 26 <220> <221> -35_signal (222) (1) .. (26) <223> 1237-1262 <400> 82 caaaaggaga gccgtggaca gtgtac 26 <210> 83 <211> 26 <212> DNA <213> Human papillomavirus type 26 <220> <221> -35_signal (222) (1) .. (26) <223> 1305-1330 <400> 83 ccgtacaggt agataaacaa tatgaa 26 <210> 84 <211> 25 <212> DNA <213> Human papillomavirus type 26 <220> <221> -35_signal (222) (1) .. (25) <223> 1345-1369 <400> 84 gcctagtgtg tgtagtcagg ggggg 25 <210> 85 <211> 26 <212> DNA <213> Human papillomavirus type 26 <220> <221> -35_signal (222) (1) .. (26) <223> 1376-1401 <400> 85 gcctcagtgg aagatatcga tgtaga 26 <210> 86 <211> 26 <212> DNA <213> Human papillomavirus type 26 <220> <221> -35_signal (222) (1) .. (26) <223> 1414-1439 <400> 86 cagtgttaca caaatatgtg aattat 26 <210> 87 <211> 26 <212> DNA <213> Human papillomavirus type 26 <220> <221> -35_signal (222) (1) .. (26) <223> 1485-1510 <400> 87 cagtatatgg tgtaagtttt gcagaa 26 <210> 88 <211> 15 <212> DNA <213> Human papillomavirus type 31 <220> <221> -35_signal (222) (1) .. (15) <223> 6593-6612 <400> 88 tgcaattgca aacag 15 <210> 89 <211> 20 <212> DNA <213> Human papillomavirus type 31 <220> <221> -35_signal (222) (1) .. (20) <223> 6593-6612 <400> 89 gcaattgcaa acagtgatac 20 <210> 90 <211> 20 <212> DNA <213> Human papillomavirus type 31 <220> <221> -35_signal (222) (1) .. (20) <223> 6594-6613 <400> 90 caattgcaaa cagtgatact 20 <210> 91 <211> 23 <212> DNA <213> Human papillomavirus type 31 <220> <221> -35_signal (222) (1) .. (23) <223> 6599-6621 <400> 91 gcaaacagtg atactacatt taa 23 <210> 92 <211> 20 <212> DNA <213> Human papillomavirus type 31 <220> <221> -35_signal (222) (1) .. (20) <223> 6612-6631 <400> 92 ctacatttaa aagtagtaat 20 <210> 93 <211> 23 <212> DNA <213> Human papillomavirus type 31 <220> <221> -35_signal (222) (1) .. (23) <223> 6691-6713 <400> 93 caaaataaca ttatctgcag aca 23 <210> 94 <211> 19 <212> DNA <213> Human papillomavirus type 31 <220> <221> -35_signal (222) (1) .. (19) <223> 6739-6757 <400> 94 tcctgctatt ttggaagat 19 <210> 95 <211> 26 <212> DNA <213> Human papillomavirus type 31 <220> <221> -35_signal (222) (1) .. (26) <223> 6769-6794 <400> 95 attgaccaca cctccctcag gttctt 26 <210> 96 <211> 22 <212> DNA <213> Human papillomavirus type 31 <220> <221> -35_signal (222) (1) .. (22) <223> 6805-6826 <400> 96 ctataggttt gtcacctcac ag 22 <210> 97 <211> 19 <212> DNA <213> Human papillomavirus type 31 <220> <221> -35_signal (222) (1) .. (19) <223> 6844-6862 <400> 97 aactgccccc caaaagccc 19 <210> 98 <211> 23 <212> DNA <213> Human papillomavirus type 31 <220> <221> -35_signal (222) (1) .. (23) <223> 1083-1105 <400> 98 agcggaggaa catgcagagg ctg 23 <210> 99 <211> 26 <212> DNA <213> Human papillomavirus type 31 <220> <221> -35_signal (222) (1) .. (26) <223> 1140-1165 <400> 99 tttaagtgat attagtagtt gtgtgg 26 <210> 100 <211> 30 <212> DNA <213> Human papillomavirus type 31 <220> <221> -35_signal (222) (1) .. (30) <223> 1177-1206 <400> 100 agtccacggt taaaagctat atgcatagaa 30 <210> 101 <211> 26 <212> DNA <213> Human papillomavirus type 31 <220> <221> -35_signal (222) (1) .. (26) <223> 1232-1257 <400> 101 gactctttga acttccagac agcggg 26 <210> 102 <211> 26 <212> DNA <213> Human papillomavirus type 31 <220> <221> -35_signal (222) (1) .. (26) <223> 1281-1306 <400> 102 gcagcagatg gtacaggtag aggagc 26 <210> 103 <211> 26 <212> DNA <213> Human papillomavirus type 31 <220> <221> -35_signal (222) (1) .. (26) <223> 1327-1352 <400> 103 aatggtagtg acgggacaca tagtga 26 <210> 104 <211> 26 <212> DNA <213> Human papillomavirus type 31 <220> <221> -35_signal (222) (1) .. (26) <223> 1369-1394 <400> 104 ccaacacgta atatattgca agtgtt 26 <210> 105 <211> 26 <212> DNA <213> Human papillomavirus type 31 <220> <221> -35_signal (222) (1) .. (26) <223> 1403-1428 <400> 105 gcaatggtaa agctgctatg ttaggt 26 <210> 106 <211> 25 <212> DNA <213> Human papillomavirus type 31 <220> <221> -35_signal (222) (1) .. (25) <223> 1441-1465 <400> 106 ttatatggtg taagttttat ggaac 25 <210> 107 <211> 20 <212> DNA <213> Human papillomavirus type 32 <220> <221> -35_signal (222) (1) .. (20) <223> 6906-6925 <400> 107 tgctactgta acaactgaag 20 <210> 108 <211> 20 <212> DNA <213> Human papillomavirus type 32 <220> <221> -35_signal (222) (1) .. (20) <223> 6907-6926 <400> 108 gctactgtaa caactgaaga 20 <210> 109 <211> 15 <212> DNA <213> Human papillomavirus type 32 <220> <221> -35_signal (222) (1) .. (15) <223> 6909-6923 <400> 109 tactgtaaca actga 15 <210> 110 <211> 20 <212> DNA <213> Human papillomavirus type 32 <220> <221> -35_signal (222) (1) .. (20) <223> 6910-6929 <400> 110 actgtaacaa ctgaagacac 20 <210> 111 <211> 22 <212> DNA <213> Human papillomavirus type 32 <220> <221> -35_signal (222) (1) .. (22) <223> 6917-6938 <400> 111 caactgaaga cacatacaag tc 22 <210> 112 <211> 23 <212> DNA <213> Human papillomavirus type 32 <220> <221> -35_signal (222) (1) .. (23) <223> 7005-7027 <400> 112 caaaattaca ttatctgtag agg 23 <210> 113 <211> 19 <212> DNA <213> Human papillomavirus type 32 <220> <221> -35_signal (222) (1) .. (19) <223> 7053-7071 <400> 113 tcctgacata ctagacgat 19 <210> 114 <211> 26 <212> DNA <213> Human papillomavirus type 32 <220> <221> -35_signal (222) (1) .. (26) <223> 7083-7108 <400> 114 tgtagctcca ccgccctctg gtactt 26 <210> 115 <211> 22 <212> DNA <213> Human papillomavirus type 32 <220> <221> -35_signal (222) (1) .. (22) <223> 7119-7140 <400> 115 ttatagattt gtgcagtctc ag 22 <210> 116 <211> 19 <212> DNA <213> Human papillomavirus type 32 <220> <221> -35_signal (222) (1) .. (19) <223> 7158-7176 <400> 116 taaggtaaca gcacctgaa 19 <210> 117 <211> 16 <212> DNA <213> Human papillomavirus type 32 <220> <221> -35_signal (222) (1) .. (16) <223> 7188-7203 <400> 117 tttttctgac tattca 16 <210> 118 <211> 23 <212> DNA <213> Human papillomavirus type 32 <220> <221> -35_signal (222) (1) .. (23) <223> 1062-1084 118 agcacatgca gataaggagg cag 23 <210> 119 <211> 30 <212> DNA <213> Human papillomavirus type 32 <220> <221> -35_signal (222) (1) .. (30) <223> 1111-1140 <400> 119 ggcagtccat atgaaagtcc cgccagtgat 30 <210> 120 <211> 26 <212> DNA <213> Human papillomavirus type 32 <220> <221> -35_signal (222) (1) .. (26) <223> 1162-1187 <400> 120 gagctaagcc ctaggcttgg tggatt 26 <210> 121 <211> 26 <212> DNA <213> Human papillomavirus type 32 <220> <221> -35_signal (222) (1) .. (26) <223> 1210-1235 <400> 121 ggggccaaac gacgactatt tcaatc 26 <210> 122 <211> 26 <212> DNA <213> Human papillomavirus type 32 <220> <221> -35_signal (222) (1) .. (26) <223> 1284-1309 <400> 122 gcaggaacag gtagaaaatg gacatg 26 <210> 123 <211> 26 <212> DNA <213> Human papillomavirus type 32 <220> <221> -35_signal (222) (1) .. (26) <223> 1352-1377 <400> 123 gtgtacatgg ggtgcaggaa aatcag 26 <210> 124 <211> 26 <212> DNA <213> Human papillomavirus type 32 <220> <221> -35_signal (222) (1) .. (26) <223> 1395-1420 <400> 124 gcctacaaca agggtggtgg aattgc 26 <210> 125 <211> 26 <212> DNA <213> Human papillomavirus type 32 <220> <221> -35_signal (222) (1) .. (26) <223> 1437-1462 <400> 125 gcaagcaaca ttgttaggta agttta 26 <210> 126 <211> 26 <212> DNA <213> Human papillomavirus type 32 <220> <221> -35_signal (222) (1) .. (26) <223> 1467-1492 <400> 126 gctgtttgga ttgtcatttg gtgatt 26 <210> 127 <211> 20 <212> DNA <213> Human papillomavirus type 33 <220> <221> -35_signal (222) (1) .. (20) <223> 6624-6643 <400> 127 tatgcacaca agtaactagt 20 <210> 128 <211> 15 <212> DNA <213> Human papillomavirus type 33 <220> <221> -35_signal (222) (1) .. (15) <223> 6628-6642 <400> 128 cacacaagta actag 15 <210> 129 <211> 20 <212> DNA <213> Human papillomavirus type 33 <220> <221> -35_signal (222) (1) .. (20) <223> 6631-6650 <400> 129 acaagtaact agtgacagta 20 <210> 130 <211> 23 <212> DNA <213> Human papillomavirus type 33 <220> <221> -35_signal (222) (1) .. (23) <223> 6635-6657 <400> 130 gtaactagtg acagtacata taa 23 <210> 131 <211> 20 <212> DNA <213> Human papillomavirus type 33 <220> <221> -35_signal (222) (1) .. (20) <223> 6648-6667 <400> 131 gtacatataa aaatgaaaat 20 <210> 132 <211> 23 <212> DNA <213> Human papillomavirus type 33 <220> <221> -35_signal (222) (1) .. (23) <223> 6727-6749 <400> 132 caaagttacc ttaactgcag aag 23 <210> 133 <211> 19 <212> DNA <213> Human papillomavirus type 33 <220> <221> -35_signal (222) (1) .. (19) <223> 6775-6793 <400> 133 tccagatatt ttagaagat 19 <210> 134 <211> 26 <212> DNA <213> Human papillomavirus type 33 <220> <221> -35_signal (222) (1) .. (26) <223> 6805-6830 <400> 134 tttaacacct cctccatctg ctagtt 26 <210> 135 <211> 22 <212> DNA <213> Human papillomavirus type 33 <220> <221> -35_signal (222) (1) .. (22) <223> 6841-6862 <400> 135 ctataggttt gttacctctc ag 22 <210> 136 <211> 19 <212> DNA <213> Human papillomavirus type 33 <220> <221> -35_signal (222) (1) .. (19) <223> 6880-6898 <400> 136 aacagtacct ccaaaggaa 19 <210> 137 <211> 16 <212> DNA <213> Human papillomavirus type 33 <220> <221> -35_signal (222) (1) .. (16) <223> 6910-6925 <400> 137 cttaggtaaa tataca 16 <210> 138 <211> 23 <212> DNA <213> Human papillomavirus type 33 <220> <221> -35_signal (222) (1) .. (23) <223> 1100-1122 <400> 138 aggggaggat gatttaaatg ctg 23 <139> <211> 26 <212> DNA <213> Human papillomavirus type 33 <220> <221> -35_signal (222) (1) .. (26) <223> 1149-1174 <400> 139 gcatgttcac aaagtgctgc ggagga 26 <210> 140 <211> 26 <212> DNA <213> Human papillomavirus type 33 <220> <221> -35_signal (222) (1) .. (26) <223> 1182-1207 <400> 140 gatcgtgctg caaacccgtg tagaac 26 <210> 141 <211> 30 <212> DNA <213> Human papillomavirus type 33 <220> <221> -35_signal (222) (1) .. (30) <223> 1227-1256 <400> 141 gaatgcacat acagaaaacg aaaaatagat 30 <210> 142 <211> 26 <212> DNA <213> Human papillomavirus type 33 <220> <221> -35_signal (222) (1) .. (26) <223> 1249-1274 <400> 142 aaatagatga gctagaagac agcgga 26 <210> 143 <211> 26 <212> DNA <213> Human papillomavirus type 33 <220> <221> -35_signal (222) (1) .. (26) <223> 1307-1332 <400> 143 ggtacaacag gtagaaagtc aaaatg 26 <210> 144 <211> 26 <212> DNA <213> Human papillomavirus type 33 <220> <221> -35_signal (222) (1) .. (26) <223> 1350-1357 <400> 144 gacttagaat ctagtggggt ggggga 26 <210> 145 <211> 26 <212> DNA <213> Human papillomavirus type 33 <220> <221> -35_signal (222) (1) .. (26) <223> 1391-1416 <400> 145 ctgtgagaca aatgtagata gctgtg 26 <210> 146 <211> 26 <212> DNA <213> Human papillomavirus type 33 <220> <221> -35_signal (222) (1) .. (26) <223> 1426-1451 <400> 146 cgttgcagga aattagtaat gttcta 26 <210> 147 <211> 23 <212> DNA <213> Human papillomavirus type 33 <220> <221> -35_signal (222) (1) .. (23) <223> 1494-1516 <400> 147 gaggcctatg gaataagttt tat 23 <210> 148 <211> 20 <212> DNA <213> Human papillomavirus type 35 <220> <221> -35_signal (222) (1) .. (20) <223> 6612-6631 <400> 148 tctgctgtgt cttctagtga 20 <210> 149 <211> 15 <212> DNA <213> Human papillomavirus type 35 <220> <221> -35_signal (222) (1) .. (15) <223> 6614-6628 <400> 149 tgctgtgtct tctag 15 <210> 150 <211> 20 <212> DNA <213> Human papillomavirus type 35 <220> <221> -35_signal (222) (1) .. (20) <223> 6618-6637 <400> 150 gtgtcttcta gtgacagtac 20 <210> 151 <211> 23 <212> DNA <213> Human papillomavirus type 35 <220> <221> -35_signal (222) (1) .. (23) <223> 6622-6644 <400> 151 cttctagtga cagtacatat aaa 23 <210> 152 <211> 20 <212> DNA <213> Human papillomavirus type 35 <220> <221> -35_signal (222) (1) .. (20) <223> 6634-6653 <400> 152 gtacatataa aaatgacaat 20 <210> 153 <211> 23 <212> DNA <213> Human papillomavirus type 35 <220> <221> -35_signal (222) (1) .. (23) <223> 6713-6735 <400> 153 taaaataaca ctaacagcag atg 23 <210> 154 <211> 19 <212> DNA <213> Human papillomavirus type 35 <220> <221> -35_signal (222) (1) .. (19) <223> 6761-6779 <400> 154 cccgtccatt ttagaggat 19 <210> 155 <211> 26 <212> DNA <213> Human papillomavirus type 35 <220> <221> -35_signal (222) (1) .. (26) <223> 6791-6816 <400> 155 ccttacacca ccgccttctg gtacct 26 <210> 156 <211> 22 <212> DNA <213> Human papillomavirus type 35 <220> <221> -35_signal (222) (1) .. (22) <223> 6827-6848 <400> 156 atatcgctat gtaacatcac ag 22 <210> 157 <211> 19 <212> DNA <213> Human papillomavirus type 35 <220> <221> -35_signal (222) (1) .. (19) <223> 6866-6884 <400> 157 acccagtgca ccaaaacct 19 <210> 158 <211> 23 <212> DNA <213> Human papillomavirus type 35 <220> <221> -35_signal (222) (1) .. (23) <223> 1089-1111 <400> 158 ggagcaaaca cacaaagagg ctg 23 <210> 159 <211> 30 <212> DNA <213> Human papillomavirus type 35 <220> <221> -35_signal (222) (1) .. (30) <223> 1151-1180 <400> 159 gcagcgtgag cttatgtgtt aataataaca 30 <210> 160 <211> 24 <212> DNA <213> Human papillomavirus type 35 <220> <221> -35_signal (222) (1) .. (24) <223> 1184-1207 <400> 160 gtccacgttt aaaagctatt tgca 24 <210> 161 <211> 26 <212> DNA <213> Human papillomavirus type 35 <220> <221> -35_signal (222) (1) .. (26) <223> 1237-1262 <400> 161 cgattatttg aactaccaga cagcgg 26 <210> 162 <211> 26 <212> DNA <213> Human papillomavirus type 35 <220> <221> -35_signal (222) (1) .. (26) <223> 1291-1316 <400> 162 gagatacaac aggtagaggg gcatga 26 <210> 163 <211> 26 <212> DNA <213> Human papillomavirus type 35 <220> <221> -35_signal (222) (1) .. (26) <223> 1338-1363 <400> 163 gggcagtggg gatagtataa cctcta 26 <210> 164 <211> 26 <212> DNA <213> Human papillomavirus type 35 <220> <221> -35_signal (222) (1) .. (26) <223> 1376-1401 <400> 164 gacatgatga gactccaacg cgagac 26 <210> 165 <211> 26 <212> DNA <213> Human papillomavirus type 35 <220> <221> -35_signal (222) (1) .. (26) <223> 1414-1439 <400> 165 ctaaaatgta gtaatgcaaa cgcagc 26 <210> 166 <211> 26 <212> DNA <213> Human papillomavirus type 35 <220> <221> -35_signal (222) (1) .. (26) <223> 1438-1463 <400> 166 gctatgttgg ctaaatttaa agaact 26 <210> 167 <211> 15 <212> DNA <213> Human papillomavirus type 37 <220> <221> -35_signal (222) (1) .. (15) <223> 6782-6796 <400> 167 tgtctactga caatg 15 <210> 168 <211> 20 <212> DNA <213> Human papillomavirus type 37 <220> <221> -35_signal (222) (1) .. (20) <223> 6782-6801 <400> 168 tgtctactga caatggcgaa 20 <210> 169 <211> 20 <212> DNA <213> Human papillomavirus type 37 <220> <221> -35_signal (222) (1) .. (20) <223> 6789-6808 <400> 169 tgacaatggc gaagttacag 20 <210> 170 <211> 20 <212> DNA <213> Human papillomavirus type 37 <220> <221> -35_signal (222) (1) .. (20) <223> 6790-6809 <400> 170 gacaatggcg aagttacaga 20 <210> 171 <211> 20 <212> DNA <213> Human papillomavirus type 37 <220> <221> -35_signal (222) (1) .. (20) <223> 6793-6812 <400> 171 aatggcgaag ttacagaata 20 <210> 172 <211> 20 <212> DNA <213> Human papillomavirus type 37 <220> <221> -35_signal (222) (1) .. (20) <223> 6806-6825 <400> 172 cagaatataa ttctcaaaca 20 <210> 173 <211> 23 <212> DNA <213> Human papillomavirus type 37 <220> <221> -35_signal (222) (1) .. (23) <223> 6885-6907 <400> 173 taaagttcct ttaaaggctg agg 23 <210> 174 <211> 19 <212> DNA <213> Human papillomavirus type 37 <220> <221> -35_signal (222) (1) .. (19) <223> 6933-6951 <400> 174 ttctggtata ttggaagag 19 <175> 175 <211> 26 <212> DNA <213> Human papillomavirus type 37 <220> <221> -35_signal (222) (1) .. (26) <223> 6963-6988 <400> 175 atttgtacct actccagata attcag 26 <210> 176 <211> 22 <212> DNA <213> Human papillomavirus type 37 <220> <221> -35_signal (222) (1) .. (22) <223> 6999-7020 <400> 176 ttataggtac attaattcaa ag 22 <210> 177 <211> 19 <212> DNA <213> Human papillomavirus type 37 <220> <221> -35_signal (222) (1) .. (19) <223> 7038-7056 <400> 177 tgcagttgtt gaaaaagaa 19 <210> 178 <211> 16 <212> DNA <213> Human papillomavirus type 37 <220> <221> -35_signal (222) (1) .. (16) <223> 7068-7083 <400> 178 ctttgcaaaa tataca 16 <210> 179 <211> 15 <212> DNA <213> Human papillomavirus type 39 <220> <221> -35_signal (222) (1) .. (15) <223> 6677-6691 <400> 179 ctctatagag tcttc 15 <210> 180 <211> 20 <212> DNA <213> Human papillomavirus type 39 <220> <221> -35_signal (222) (1) .. (20) <223> 6682-6701 <400> 180 tagagtcttc cataccttct 20 <210> 181 <211> 20 <212> DNA <213> Human papillomavirus type 39 <220> <221> -35_signal (222) (1) .. (20) <223> 6681-6700 <400> 181 atagagtctt ccataccttc 20 <210> 182 <211> 23 <212> DNA <213> Human papillomavirus type 39 <220> <221> -35_signal (222) (1) .. (23) <223> 6686-6708 <400> 182 gtcttccata ccttctacat atg 23 <210> 183 <211> 20 <212> DNA <213> Human papillomavirus type 39 <220> <221> -35_signal (222) (1) .. (20) <223> 6700-6719 <400> 183 ctacatatga tccttctaag 20 <210> 184 <211> 23 <212> DNA <213> Human papillomavirus type 39 <220> <221> -35_signal (222) (1) .. (23) <223> 6779-6801 <400> 184 tactgtcaca ttaacaactg atg 23 <210> 185 <211> 18 <212> DNA <213> Human papillomavirus type 39 <220> <221> -35_signal (222) (1) .. (18) <223> 6827-6844 <400> 185 ttcctctata ttggacaa 18 <210> 186 <211> 26 <212> DNA <213> Human papillomavirus type 39 <220> <221> -35_signal (222) (1) .. (26) <223> 6857-6882 <400> 186 tgtagctcct ccaccatctg ccagtt 26 <210> 187 <211> 22 <212> DNA <213> Human papillomavirus type 39 <220> <221> -35_signal (222) (1) .. (22) <223> 6893-6914 <400> 187 ttacagatac ctacagtctg ca 22 <210> 188 <211> 19 <212> DNA <213> Human papillomavirus type 39 <220> <221> -35_signal (222) (1) .. (19) <223> 6932-6950 <400> 188 ggatgctcca gcacctgaa 19 <210> 189 <211> 16 <212> DNA <213> Human papillomavirus type 39 <220> <221> -35_signal (222) (1) .. (16) <223> 6962-6977 <400> 189 atatgacggt ctaaag 16 <210> 190 <211> 23 <212> DNA <213> Human papillomavirus type 39 <220> <221> -35_signal (222) (1) .. (23) <223> 1149-1171 <400> 190 ggcccaaagg gatgcacaag cag 23 <210> 191 <211> 30 <212> DNA <213> Human papillomavirus type 39 <220> <221> -35_signal (222) (1) .. (30) <223> 1196-1225 <400> 191 cagacagcag tggcgacact agaccgtatg 30 <210> 192 <211> 25 <212> DNA <213> Human papillomavirus type 39 <220> <221> -35_signal (222) (1) .. (25) <223> 1283-1308 <400> 192 gtaggcagga ataccagggg aacac 25 <210> 193 <211> 26 <212> DNA <213> Human papillomavirus type 39 <220> <221> -35_signal (222) (1) .. (26) <223> 1283-1308 <400> 193 gcagtacgca ggcaacacaa acggtg 26 <210> 194 <211> 26 <212> DNA <213> Human papillomavirus type 39 <220> <221> -35_signal (222) (1) .. (26) <223> 1362-1387 <400> 194 ggaggaggta actgtagcaa ctaata 26 <210> 195 <211> 26 <212> DNA <213> Human papillomavirus type 39 <220> <221> -35_signal (222) (1) .. (26) <223> 1411-1436 <400> 195 catggcggca gtgtacggga ggagtg 26 <210> 196 <211> 26 <212> DNA <213> Human papillomavirus type 39 <220> <221> -35_signal (222) (1) .. (26) <223> 1446-1471 <400> 196 ggatagtgct atagatagtg aaaacc 26 <210> 197 <211> 26 <212> DNA <213> Human papillomavirus type 39 <220> <221> -35_signal (222) (1) .. (26) <223> 1484-1509 <400> 197 ctccaactgc acaaattaaa ttattg 26 <210> 198 <211> 26 <212> DNA <213> Human papillomavirus type 39 <220> <221> -35_signal (222) (1) .. (26) <223> 1517-1542 <400> 198 ccaataacaa aaaggctgca atgcta 26 <210> 199 <211> 15 <212> DNA <213> Human papillomavirus type 40 <220> <221> -35_signal (222) (1) .. (15) <223> 6719-6733 <400> 199 tgccactaca cagtc 15 <210> 200 <211> 20 <212> DNA <213> Human papillomavirus type 40 <220> <221> -35_signal (222) (1) .. (20) <223> 6724-6743 <400> 200 ctacacagtc ccctccgtct 20 <210> 201 <211> 20 <212> DNA <213> Human papillomavirus type 40 <220> <221> -35_signal (222) (1) .. (20) <223> 6719-6738 <400> 201 tgccactaca cagtcccctc 20 <210> 202 <211> 22 <212> DNA <213> Human papillomavirus type 40 <220> <221> -35_signal (222) (1) .. (22) <223> 6729-6750 <400> 202 cagtcccctc cgtctacata ta 22 <210> 203 <211> 20 <212> DNA <213> Human papillomavirus type 40 <220> <221> -35_signal (222) (1) .. (20) <223> 6742-6761 <400> 203 ctacatatac tagtgaacaa 20 <210> 204 <211> 23 <212> DNA <213> Human papillomavirus type 40 <220> <221> -35_signal (222) (1) .. (23) <223> 6821-6843 <400> 204 tagtattacc ttaacggcgg agg 23 <210> 205 <211> 19 <212> DNA <213> Human papillomavirus type 40 <220> <221> -35_signal (222) (1) .. (19) <223> 6869-6887 <400> 205 tgctggtatt ttagaacag 19 <206> 206 <211> 26 <212> DNA <213> Human papillomavirus type 40 <220> <221> -35_signal (222) (1) .. (26) <223> 6899-6924 <400> 206 gttgtcgccg cccccaaatg gtacct 26 <210> 207 <211> 22 <212> DNA <213> Human papillomavirus type 40 <220> <221> -35_signal (222) (1) .. (22) <223> 6935-6959 <400> 207 atacagatat gtgcagtccc ag 22 <210> 208 <211> 19 <212> DNA <213> Human papillomavirus type 40 <220> <221> -35_signal (222) (1) .. (19) <223> 6974-6992 <400> 208 gccaccccct gaaaaggca 19 <210> 209 <211> 16 <212> DNA <213> Human papillomavirus type 40 <220> <221> -35_signal (222) (1) .. (16) <223> 7004-7019 <400> 209 ctatgcaaaa ttaagt 16 <210> 210 <211> 23 <212> DNA <213> Human papillomavirus type 40 <220> <221> -35_signal (222) (1) .. (23) <223> 1038-1060 <400> 210 ggcggatgct cattatgcgg ctg 23 <210> 211 <211> 30 <212> DNA <213> Human papillomavirus type 40 <220> <221> -35_signal (222) (1) .. (30) <223> 1087-1116 <400> 211 ggtagtccat atgttagtcc tttaagtaat 30 <210> 212 <211> 26 <212> DNA <213> Human papillomavirus type 40 <220> <221> -35_signal (222) (1) .. (26) <223> 1148-1173 <400> 212 cacggctgga cgctataaca ttaagt 26 <210> 213 <211> 26 <212> DNA <213> Human papillomavirus type 40 <220> <221> -35_signal (222) (1) .. (26) <223> 1196-1221 <400> 213 gacggctgtt tgacagacca gaatta 26 <210> 214 <211> 26 <212> DNA <213> Human papillomavirus type 40 <220> <221> -35_signal (222) (1) .. (26) <223> 1255-1280 <400> 214 gctgaaacgc aggtagagag aaatgg 26 <210> 215 <211> 26 <212> DNA <213> Human papillomavirus type 40 <220> <221> -35_signal (222) (1) .. (26) <223> 1299-1324 <400> 215 gggaggtgga caaggaaggg acacag 26 <210> 216 <211> 26 <212> DNA <213> Human papillomavirus type 40 <220> <221> -35_signal (222) (1) .. (26) <223> 1347-1372 <400> 216 ggaagtgcag acacatagca acacac 26 <210> 217 <211> 26 <212> DNA <213> Human papillomavirus type 40 <220> <221> -35_signal (222) (1) .. (26) <223> 1395-1420 <400> 217 ggtactagaa ctattgaaat gtaaga 26 <210> 218 <211> 26 <212> DNA <213> Human papillomavirus type 40 <220> <221> -35_signal (222) (1) .. (26) <223> 1437-1462 <400> 218 gcttggtaag tttaaggatt gctatg 26 <210> 219 <211> 20 <212> DNA <213> Human papillomavirus type 45 <220> <221> -35_signal (222) (1) .. (20) <223> 6651-6670 <400> 219 tgcctctaca caaaatcctg 20 <210> 220 <211> 15 <212> DNA <213> Human papillomavirus type 45 <220> <221> -35_signal (222) (1) .. (15) <223> 6654-6668 <400> 220 ctctacacaa aatcc 15 <210> 221 <211> 20 <212> DNA <213> Human papillomavirus type 45 <220> <221> -35_signal (222) (1) .. (20) <223> 6660-6679 <400> 221 acaaaatcct gtgccaagta 20 <210> 222 <211> 20 <212> DNA <213> Human papillomavirus type 45 <220> <221> -35_signal (222) (1) .. (20) <223> 6661-6680 <400> 222 caaaatcctg tgccaagtac 20 <210> 223 <211> 22 <212> DNA <213> Human papillomavirus type 45 <220> <221> -35_signal (222) (1) .. (22) <223> 6664-6685 <400> 223 aatcctgtgc caagtacata tg 22 <210> 224 <211> 20 <212> DNA <213> Human papillomavirus type 45 <220> <221> -35_signal (222) (1) .. (20) <223> 6677-6696 <400> 224 gtacatatga ccctactaag 20 <210> 225 <211> 23 <212> DNA <213> Human papillomavirus type 45 <220> <221> -35_signal (222) (1) .. (23) <223> 6756-6778 <400> 225 cactattact ttaactgcag agg 23 <210> 226 <211> 19 <212> DNA <213> Human papillomavirus type 45 <220> <221> -35_signal (222) (1) .. (19) <223> 6804-6822 <400> 226 tagtagtata ttagaaaat 19 <210> 227 <211> 26 <212> DNA <213> Human papillomavirus type 45 <220> <221> -35_signal (222) (1) .. (26) <223> 6834-6859 <400> 227 tgtccctcca ccacctacta caagtt 26 <210> 228 <211> 22 <212> DNA <213> Human papillomavirus type 45 <220> <221> -35_signal (222) (1) .. (22) <223> 6870-6891 <400> 228 atatcgtttt gtgcaatcag tt 22 <210> 229 <211> 19 <212> DNA <213> Human papillomavirus type 45 <220> <221> -35_signal (222) (1) .. (19) <223> 6909-6927 <400> 229 ggatactaca cctccagaa 19 <210> 230 <211> 23 <212> DNA <213> Human papillomavirus type 45 <220> <221> -35_signal (222) (1) .. (23) <223> 1135-1157 <400> 230 agttcagaat gatgcacagg tgt 23 <210> 231 <211> 29 <212> DNA <213> Human papillomavirus type 45 <220> <221> -35_signal (222) (1) .. (29) <223> 1216-1245 <400> 231 gcagctaagt gtggatacgg atctaagcc 29 <210> 232 <211> 26 <212> DNA <213> Human papillomavirus type 45 <220> <221> -35_signal (222) (1) .. (26) <223> 1253-1278 <400> 232 caagaaattt cattaaatag tgggca 26 <210> 233 <211> 26 <212> DNA <213> Human papillomavirus type 45 <220> <221> -35_signal (222) (1) .. (26) <223> 1294-1319 <400> 233 acggttgttt acaatatcag atagtg 26 <210> 234 <211> 26 <212> DNA <213> Human papillomavirus type 45 <220> <221> -35_signal (222) (1) .. (26) <223> 1397-1422 <400> 234 catagtacac aaagtagtgg tgggga 26 <210> 235 <211> 26 <212> DNA <213> Human papillomavirus type 45 <220> <221> -35_signal (222) (1) .. (26) <223> 1430-1455 <400> 235 gacaatgcag aaaatgtaga tccgca 26 <210> 236 <211> 26 <212> DNA <213> Human papillomavirus type 45 <220> <221> -35_signal (222) (1) .. (26) <223> 1477-1502 <400> 236 ggagctatta caagcaagta acaaaa 26 <210> 237 <211> 25 <212> DNA <213> Human papillomavirus type 45 <220> <221> -35_signal (222) (1) .. (25) <223> 1508-1532 <400> 237 gcaatgctgg cagtatttaa agaca 25 <210> 238 <211> 26 <212> DNA <213> Human papillomavirus type 45 <220> <221> -35_signal (222) (1) .. (26) <223> 1534-1559 <400> 238 atatgggctg tcatttacgg atttgg 26 <210> 239 <211> 20 <212> DNA <213> Human papillomavirus type 51 <220> <221> -35_signal (222) (1) .. (20) <223> 6555-6574 <400> 239 cactgccact gctgcggttt 20 <210> 240 <211> 15 <212> DNA <213> Human papillomavirus type 51 <220> <221> -35_signal (222) (1) .. (15) <223> 6558-6572 <400> 240 tgccactgct gcggt 15 <210> 241 <211> 20 <212> DNA <213> Human papillomavirus type 51 <220> <221> -35_signal (222) (1) .. (20) <223> 6561-6580 <400> 241 cactgctgcg gtttccccaa 20 <210> 242 <211> 20 <212> DNA <213> Human papillomavirus type 51 <220> <221> -35_signal (222) (1) .. (20) <223> 6560-6579 <400> 242 ccactgctgc ggtttcccca 20 <210> 243 <211> 22 <212> DNA <213> Human papillomavirus type 51 <220> <221> -35_signal (222) (1) .. (22) <223> 6566-6587 <400> 243 ctgcggtttc cccaacattt ac 22 <210> 244 <211> 20 <212> DNA <213> Human papillomavirus type 51 <220> <221> -35_signal (222) (1) .. (20) <223> 6578-6597 <400> 244 caacatttac tccaagtaac 20 <210> 245 <211> 23 <212> DNA <213> Human papillomavirus type 51 <220> <221> -35_signal (222) (1) .. (23) <223> 6657-6679 <400> 245 taaaattact ttaactacag agg 23 <210> 246 <211> 19 <212> DNA <213> Human papillomavirus type 51 <220> <221> -35_signal (222) (1) .. (19) <223> 6705-6723 <400> 246 tcctaccatt cttgaacag 19 <210> 247 <211> 26 <212> DNA <213> Human papillomavirus type 51 <220> <221> -35_signal (222) (1) .. (26) <223> 6735-6760 <400> 247 attaacatta cctccgtctg ctagtt 26 <210> 248 <211> 22 <212> DNA <213> Human papillomavirus type 51 <220> <221> -35_signal (222) (1) .. (22) <223> 6771-6792 <400> 248 atataggttt gttagaaatg ca 22 <210> 249 <211> 19 <212> DNA <213> Human papillomavirus type 51 <220> <221> -35_signal (222) (1) .. (19) <223> 6810-6828 <400> 249 ggacacccct ccacaggct 19 <210> 250 <211> 16 <212> DNA <213> Human papillomavirus type 51 <220> <221> -35_signal (222) (1) .. (16) <223> 6840-6855 <400> 250 tttggccaaa tataaa 16 <210> 251 <211> 23 <212> DNA <213> Human papillomavirus type 51 <220> <221> -35_signal (222) (1) .. (23) <223> 1092-1114 <400> 251 attacaggca aacaaagagg ctg 23 <210> 252 <211> 30 <212> DNA <213> Human papillomavirus type 51 <220> <221> -35_signal (222) (1) .. (30) <223> 1149-1178 <400> 252 gcgaagcagc ccattaggag acattacaaa 30 <210> 253 <211> 26 <212> DNA <213> Human papillomavirus type 51 <220> <221> -35_signal (222) (1) .. (26) <223> 1197-1222 <400> 253 ccatagtcag gcaaacgagt cacaag 26 <210> 254 <211> 25 <212> DNA <213> Human papillomavirus type 51 <220> <221> -35_signal (222) (1) .. (25) <223> 1231-1255 <400> 254 agattactgg acagttatcc ggaca 25 <210> 255 <211> 26 <212> DNA <213> Human papillomavirus type 51 <220> <221> -35_signal (222) (1) .. (26) <223> 1300-1325 <400> 255 caggtagatg ggcaacatgg cggttc 26 <210> 256 <211> 26 <212> DNA <213> Human papillomavirus type 51 <220> <221> -35_signal (222) (1) .. (26) <223> 1384-1409 <400> 256 agctgtgcaa atgtagaact aaacag 26 <210> 257 <211> 26 <212> DNA <213> Human papillomavirus type 51 <220> <221> -35_signal (222) (1) .. (26) <223> 1477-1502 <400> 257 ggtattagtt ataatgagtt ggtacg 26 <210> 258 <211> 15 <212> DNA <213> Human papillomavirus type 52 <220> <221> -35_signal (222) (1) .. (15) <223> 6695-6709 <400> 258 tgaggttaaa aagga 15 <210> 259 <211> 20 <212> DNA <213> Human papillomavirus type 52 <220> <221> -35_signal (222) (1) .. (20) <223> 6695-6714 <400> 259 tgaggttaaa aaggaaagca 20 <210> 260 <211> 20 <212> DNA <213> Human papillomavirus type 52 <220> <221> -35_signal (222) (1) .. (20) <223> 6696-6715 <400> 260 gaggttaaaa aggaaagcac 20 <210> 261 <211> 20 <212> DNA <213> Human papillomavirus type 52 <220> <221> -35_signal (222) (1) .. (20) <223> 6700-6719 <400> 261 ttaaaaagga aagcacatat 20 <210> 262 <211> 22 <212> DNA <213> Human papillomavirus type 52 <220> <221> -35_signal (222) (1) .. (22) <223> 6704-6725 <400> 262 aaaggaaagc acatataaaa at 22 <210> 263 <211> 20 <212> DNA <213> Human papillomavirus type 52 <220> <221> -35_signal (222) (1) .. (20) <223> 6712-6731 <400> 263 gcacatataa aaatgaaaat 20 <210> 264 <211> 23 <212> DNA <213> Human papillomavirus type 52 <220> <221> -35_signal (222) (1) .. (23) <223> 6791-6813 <400> 264 caaaattaca ttaacagctg atg 23 <210> 265 <211> 19 <212> DNA <213> Human papillomavirus type 52 <220> <221> -35_signal (222) (1) .. (19) <223> 6839-6857 <400> 265 tgccactatt ttagaggac 19 <210> 266 <211> 26 <212> DNA <213> Human papillomavirus type 52 <220> <221> -35_signal (222) (1) .. (26) <223> 6869-6894 <400> 266 ccttacccca ccaccgtctg catctt 26 <210> 267 <211> 22 <212> DNA <213> Human papillomavirus type 52 <220> <221> -35_signal (222) (1) .. (22) <223> 6905-6926 <400> 267 atacagattt gtcacttcta ct 22 <210> 268 <211> 19 <212> DNA <213> Human papillomavirus type 52 <220> <221> -35_signal (222) (1) .. (19) <223> 6944-6962 <400> 268 aaacacacca cctaaagga 19 <210> 269 <211> 16 <212> DNA <213> Human papillomavirus type 52 <220> <221> -35_signal (222) (1) .. (16) <223> 6974-6989 <400> 269 tttaaaggac tatatg 16 <210> 270 <211> 23 <212> DNA <213> Human papillomavirus type 52 <220> <221> -35_signal (222) (1) .. (23) <223> 1085-1107 <400> 270 aggggaggat gatttacatg ctg 23 <210> 271 <211> 30 <212> DNA <213> Human papillomavirus type 52 <220> <221> -35_signal (222) (1) .. (30) <223> 1135-1164 <400> 271 gcagtccgga aagtgctggg caagatggtg 30 <210> 272 <211> 26 <212> DNA <213> Human papillomavirus type 52 <220> <221> -35_signal (222) (1) .. (26) <223> 1176-1201 <400> 272 ggtagtccgc gtgcaaaaca catttg 26 <210> 273 <211> 26 <212> DNA <213> Human papillomavirus type 52 <220> <221> -35_signal (222) (1) .. (26) <223> 1224-1249 <400> 273 ccaaaacgca aaccatgtca cgtaga 26 <210> 274 <211> 22 <212> DNA <213> Human papillomavirus type 52 <220> <221> -35_signal (222) (1) .. (22) <223> 1311-1332 <400> 274 caaaatggcg actggcaaag ta 22 <210> 275 <211> 26 <212> DNA <213> Human papillomavirus type 52 <220> <221> -35_signal (222) (1) .. (26) <223> 1359-1384 <400> 275 gctagtaatt cagatgtaag ttgtac 26 <210> 276 <211> 26 <212> DNA <213> Human papillomavirus type 52 <220> <221> -35_signal (222) (1) .. (26) <223> 1401-1426 <400> 276 gaggaaaata gtaatagaac gctaaa 26 <210> 277 <211> 26 <212> DNA <213> Human papillomavirus type 52 <220> <221> -35_signal (222) (1) .. (26) <223> 1447-1472 <400> 277 gcgaaaatag cataaaaaca actgta 26 <210> 278 <211> 26 <212> DNA <213> Human papillomavirus type 52 <220> <221> -35_signal (222) (1) .. (26) <223> 1487-1512 <400> 278 agaaacatat ggtgttagct ttatgg 26 <210> 279 <211> 20 <212> DNA <213> Human papillomavirus type 53 <220> <221> -35_signal (222) (1) .. (20) <223> 6681-6700 <400> 279 tccgcaacca cacagtctat 20 <210> 280 <211> 15 <212> DNA <213> Human papillomavirus type 53 <220> <221> -35_signal (222) (1) .. (15) <223> 6682-6696 <400> 280 ccgcaaccac acagt 15 <210> 281 <211> 20 <212> DNA <213> Human papillomavirus type 53 <220> <221> -35_signal (222) (1) .. (20) <223> 6682-6701 <400> 281 ccgcaaccac acagtctatg 20 <210> 282 <211> 22 <212> DNA <213> Human papillomavirus type 53 <220> <221> -35_signal (222) (1) .. (22) <223> 6691-6712 <400> 282 cacagtctat gtctacatat aa 22 <210> 283 <211> 20 <212> DNA <213> Human papillomavirus type 53 <220> <221> -35_signal (222) (1) .. (20) <223> 6703-6722 <400> 283 ctacatataa ttcaaagcaa 20 <210> 284 <211> 23 <212> DNA <213> Human papillomavirus type 53 <220> <221> -35_signal (222) (1) .. (23) <223> 6782-6804 <400> 284 taaaatatcc ctgtctgctg agg 23 <210> 285 <211> 19 <212> DNA <213> Human papillomavirus type 53 <220> <221> -35_signal (222) (1) .. (19) <223> 6830-6848 <400> 285 ttctacctta ctggaagac 19 <210> 286 <211> 26 <212> DNA <213> Human papillomavirus type 53 <220> <221> -35_signal (222) (1) .. (26) <223> 6860-6885 <400> 286 tttgtcgcct cctgttgcca ctagct 26 <210> 287 <211> 22 <212> DNA <213> Human papillomavirus type 53 <220> <221> -35_signal (222) (1) .. (22) <223> 6896-6917 <400> 287 atacagatat gtgaaaagtg ca 22 <210> 288 <211> 19 <212> DNA <213> Human papillomavirus type 53 <220> <221> -35_signal (222) (1) .. (19) <223> 6935-6953 <400> 288 ggatcagccc cctcctgaa 19 <210> 289 <211> 15 <212> DNA <213> Human papillomavirus type 54 <220> <221> -35_signal (222) (1) .. (15) <223> 6633-6647 <400> 289 tacagcatcc acgca 15 <210> 290 <211> 20 <212> DNA <213> Human papillomavirus type 54 <220> <221> -35_signal (222) (1) .. (20) <223> 6635-6654 <400> 290 cagcatccac gcaggatagc 20 <210> 291 <211> 20 <212> DNA <213> Human papillomavirus type 54 <220> <221> -35_signal (222) (1) .. (20) <223> 6643-6662 <400> 291 acgcaggata gctttaataa 20 <210> 292 <211> 20 <212> DNA <213> Human papillomavirus type 54 <220> <221> -35_signal (222) (1) .. (20) <223> 6642-6661 <400> 292 cacgcaggat agctttaata 20 <210> 293 <211> 20 <212> DNA <213> Human papillomavirus type 54 <220> <221> -35_signal (222) (1) .. (20) <223> 6650-6669 <400> 293 atagctttaa taattctgac 20 <210> 294 <211> 23 <212> DNA <213> Human papillomavirus type 54 <220> <221> -35_signal (222) (1) .. (23) <223> 6729-6751 <400> 294 taccataacc cttacagcag atg 23 <210> 295 <211> 19 <212> DNA <213> Human papillomavirus type 54 <220> <221> -35_signal (222) (1) .. (19) <223> 6777-6795 <400> 295 tcccactatt ctagaggac 19 <210> 296 <211> 26 <212> DNA <213> Human papillomavirus type 54 <220> <221> -35_signal (222) (1) .. (26) <223> 6807-6832 <400> 296 tataaccccc ccagctacaa gtagtt 26 <210> 297 <211> 22 <212> DNA <213> Human papillomavirus type 54 <220> <221> -35_signal (222) (1) .. (22) <223> 6843-6864 <400> 297 atataggttt gtacagtcac ag 22 <210> 298 <211> 22 <212> DNA <213> Human papillomavirus type 54 <220> <221> -35_signal (222) (1) .. (22) <223> 6882-6903 <400> 298 gaataatgcc cctgcaaagg aa 22 <210> 299 <211> 23 <212> DNA <213> Human papillomavirus type 54 <220> <221> -35_signal (222) (1) .. (23) <223> 1043-1065 <400> 299 gctgcaggca gatgtagagg cag 23 <210> 300 <211> 30 <212> DNA <213> Human papillomavirus type 54 <220> <221> -35_signal (222) (1) .. (30) <223> 1099-1128 <400> 300 cgtatgtaag tcctgttgca aacagcgaac 30 <210> 301 <211> 20 <212> DNA <213> Human papillomavirus type 54 <220> <221> -35_signal (222) (1) .. (20) <223> 1130-1149 <400> 301 ctgtgtagaa aaggacctaa 20 <210> 302 <211> 26 <212> DNA <213> Human papillomavirus type 54 <220> <221> -35_signal (222) (1) .. (26) <223> 1166-1191 <400> 302 tatatcccta ggacggcggt cagcca 26 <210> 303 <211> 26 <212> DNA <213> Human papillomavirus type 54 <220> <221> -35_signal (222) (1) .. (26) <223> 1265-1290 <400> 303 ggtaaatacc gaggggacag atgaaa 26 <210> 304 <211> 26 <212> DNA <213> Human papillomavirus type 54 <220> <221> -35_signal (222) (1) .. (26) <223> 1323-1348 <400> 304 gaaacaacta cagatagcct aggaag 26 <210> 305 <211> 26 <212> DNA <213> Human papillomavirus type 54 <220> <221> -35_signal (222) (1) .. (26) <223> 1386-1411 <400> 305 cgtgtagcat tgtttggtat gtttaa 26 <210> 306 <211> 22 <212> DNA <213> Human papillomavirus type 54 <220> <221> -35_signal (222) (1) .. (22) <223> 1419-1440 <400> 306 tatggattaa gttttatgga cc 22 <210> 307 <211> 15 <212> DNA <213> Human papillomavirus type 56 <220> <221> -35_signal (222) (1) .. (15) <223> 6630-6644 <400> 307 ctgctacaga acagt 15 <210> 308 <211> 20 <212> DNA <213> Human papillomavirus type 56 <220> <221> -35_signal (222) (1) .. (20) <223> 6632-6651 <400> 308 gctacagaac agttaagtaa 20 <210> 309 <211> 20 <212> DNA <213> Human papillomavirus type 56 <220> <221> -35_signal (222) (1) .. (20) <223> 6636-6655 <400> 309 cagaacagtt aagtaaatat 20 <210> 310 <211> 23 <212> DNA <213> Human papillomavirus type 56 <220> <221> -35_signal (222) (1) .. (23) <223> 6638-6660 <400> 310 gaacagttaa gtaaatatga tgc 23 <210> 311 <211> 20 <212> DNA <213> Human papillomavirus type 56 <220> <221> -35_signal (222) (1) .. (20) <223> 6648-6667 <400> 311 gtaaatatga tgcacgaaaa 20 <210> 312 <211> 23 <212> DNA <213> Human papillomavirus type 56 <220> <221> -35_signal (222) (1) .. (23) <223> 6727-6749 <400> 312 caaaattact ttgtctgcag agg 23 <210> 313 <211> 19 <212> DNA <213> Human papillomavirus type 56 <220> <221> -35_signal (222) (1) .. (19) <223> 6775-6793 <400> 313 tgctaaccta ctggaggac 19 <210> 314 <211> 26 <212> DNA <213> Human papillomavirus type 56 <220> <221> -35_signal (222) (1) .. (26) <223> 6805-5830 <400> 314 gttatccccg ccagtggcca ccagcc 26 <210> 315 <211> 22 <212> DNA <213> Human papillomavirus type 56 <220> <221> -35_signal (222) (1) .. (22) <223> 6841-6862 <400> 315 atatagatat gttagaagca ca 22 <210> 316 <211> 19 <212> DNA <213> Human papillomavirus type 56 <220> <221> -35_signal (222) (1) .. (19) <223> 6880-6898 <400> 316 ggaacagcca ccaacagaa 19 <210> 317 <211> 20 <212> DNA <213> Human papillomavirus type 58 <220> <221> -35_signal (222) (1) .. (20) <223> 6674-6693 <400> 317 atgcactgaa gtaactaagg 20 <210> 318 <211> 20 <212> DNA <213> Human papillomavirus type 58 <220> <221> -35_signal (222) (1) .. (20) <223> 6677-6696 <400> 318 cactgaagta actaaggaag 20 <210> 319 <211> 15 <212> DNA <213> Human papillomavirus type 58 <220> <221> -35_signal (222) (1) .. (15) <223> 6680-6694 <400> 319 tgaagtaact aagga 15 <210> 320 <211> 20 <212> DNA <213> Human papillomavirus type 58 <220> <221> -35_signal (222) (1) .. (20) <223> 6681-6700 <400> 320 gaagtaacta aggaaggtac 20 <210> 321 <211> 22 <212> DNA <213> Human papillomavirus type 58 <220> <221> -35_signal (222) (1) .. (22) <223> 6688-6709 <400> 321 ctaaggaagg tacatataaa aa 22 <210> 322 <211> 20 <212> DNA <213> Human papillomavirus type 58 <220> <221> -35_signal (222) (1) .. (20) <223> 6703-6722 <400> 322 ataaaaatga taattttaag 20 <210> 323 <211> 23 <212> DNA <213> Human papillomavirus type 58 <220> <221> -35_signal (222) (1) .. (23) <223> 6776-6798 <400> 323 caaaattaca ctaactgcag aga 23 <210> 324 <211> 19 <212> DNA <213> Human papillomavirus type 58 <220> <221> -35_signal (222) (1) .. (19) <223> 6824-6842 <400> 324 ttccaatatt ttggaggac 19 <210> 325 <211> 26 <212> DNA <213> Human papillomavirus type 58 <220> <221> -35_signal (222) (1) .. (26) <223> 6854-6879 <400> 325 tttaacacct cctccgtctg ccagtt 26 <210> 326 <211> 22 <212> DNA <213> Human papillomavirus type 58 <220> <221> -35_signal (222) (1) .. (22) <223> 6890-6911 <400> 326 atatagattt gttacctccc ag 22 <210> 327 <211> 19 <212> DNA <213> Human papillomavirus type 58 <220> <221> -35_signal (222) (1) .. (19) <223> 6929-6947 <400> 327 aacagcaccc cctaaagaa 19 <210> 328 <211> 23 <212> DNA <213> Human papillomavirus type 58 <220> <221> -35_signal (222) (1) .. (23) <223> 1104-1126 <400> 328 aggggtggac gatataaatg ctg 23 <210> 329 <211> 23 <212> DNA <213> Human papillomavirus type 58 <220> <221> -35_signal (222) (1) .. (23) <223> 1153-1175 <400> 329 gcatgctcag aaagtgctgt aga 23 <210> 330 <211> 30 <212> DNA <213> Human papillomavirus type 58 <220> <221> -35_signal (222) (1) .. (30) <223> 1195-1224 <400> 330 gcaaatgtgt gtgtatcgtg gaaatataaa 30 <210> 331 <211> 22 <212> DNA <213> Human papillomavirus type 58 <220> <221> -35_signal (222) (1) .. (22) <223> 1253-1274 <400> 331 aaattattga gctagaagac ag 22 <210> 332 <211> 21 <212> DNA <213> Human papillomavirus type 58 <220> <221> -35_signal (222) (1) .. (21) <223> 1312-1332 <400> 332 gcacaccagg tagaaagcca a 21 <210> 333 <211> 26 <212> DNA <213> Human papillomavirus type 58 <220> <221> -35_signal (222) (1) .. (26) <223> 1377-1402 <400> 333 ggctagttca gatgtaagca gtgaaa 26 <210> 334 <211> 22 <212> DNA <213> Human papillomavirus type 58 <220> <221> -35_signal (222) (1) .. (22) <223> 1445-1466 <400> 334 gtaatattct acataacagt aa 22 <210> 335 <211> 21 <212> DNA <213> Human papillomavirus type 58 <220> <221> -35_signal (222) (1) .. (21) <223> 1474-1494 <400> 335 gcaacgctat tatataaatt c 21 <210> 336 <211> 24 <212> DNA <213> Human papillomavirus type 58 <220> <221> -35_signal (222) (1) .. (24) <223> 1501-1524 <400> 336 gcttatggag taagttttat ggaa 24 <210> 337 <211> 15 <212> DNA <213> Human papillomavirus type 59 <220> <221> -35_signal (222) (1) .. (15) <223> 6643-6657 <400> 337 ttctactact tcttc 15 <210> 338 <211> 20 <212> DNA <213> Human papillomavirus type 59 <220> <221> -35_signal (222) (1) .. (20) <223> 6647-6666 <400> 338 actacttctt ctattcctaa 20 <210> 339 <211> 20 <212> DNA <213> Human papillomavirus type 59 <220> <221> -35_signal (222) (1) .. (20) <223> 6650-6669 <400> 339 acttcttcta ttcctaatgt 20 <210> 340 <211> 23 <212> DNA <213> Human papillomavirus type 59 <220> <221> -35_signal (222) (1) .. (23) <223> 6653-6675 <400> 340 tcttctattc ctaatgtata cac 23 <210> 341 <211> 20 <212> DNA <213> Human papillomavirus type 59 <220> <221> -35_signal (222) (1) .. (20) <223> 6666-6685 <400> 341 atgtatacac acctaccagt 20 <210> 342 <211> 23 <212> DNA <213> Human papillomavirus type 59 <220> <221> -35_signal (222) (1) .. (23) <223> 6745-6767 <400> 342 taaaataaca ttaactacag agg 23 <210> 343 <211> 19 <212> DNA <213> Human papillomavirus type 59 <220> <221> -35_signal (222) (1) .. (19) <223> 6793-6811 <400> 343 taccactatt ttggaggat 19 <210> 344 <211> 26 <212> DNA <213> Human papillomavirus type 59 <220> <221> -35_signal (222) (1) .. (26) <223> 6823-6848 <400> 344 tgttacacca cctcctactg ctagtt 26 <210> 345 <211> 22 <212> DNA <213> Human papillomavirus type 59 <220> <221> -35_signal (222) (1) .. (22) <223> 6859-6880 <400> 345 ataccgtttt gttcaatctg ct 22 <210> 346 <211> 19 <212> DNA <213> Human papillomavirus type 59 <220> <221> -35_signal (222) (1) .. (19) <223> 6898-6916 <400> 346 ggacaccgca ccgccagtt 19 <210> 347 <211> 16 <212> DNA <213> Human papillomavirus type 59 <220> <221> -35_signal (222) (1) .. (16) <223> 6928-6943 <400> 347 ttatgacaaa ctaaag 16 <210> 348 <211> 23 <212> DNA <213> Human papillomavirus type 59 <220> <221> -35_signal (222) (1) .. (23) <223> 1093-1115 <400> 348 agcccaaagg gatgcacggg aaa 23 <210> 349 <211> 30 <212> DNA <213> Human papillomavirus type 59 <220> <221> -35_signal (222) (1) .. (30) <223> 1143-1172 <400> 349 gcagtataga aaacagtagt gagaaagcgg 30 <210> 350 <211> 25 <212> DNA <213> Human papillomavirus type 59 <220> <221> -35_signal (222) (1) .. (25) <223> 1196-1220 <400> 350 ccattacaag aaatatcagt aaatg 25 <210> 351 <211> 24 <212> DNA <213> Human papillomavirus type 59 <220> <221> -35_signal (222) (1) .. (24) <223> 1245-1268 <400> 351 ggttaataac agtgccagac agcg 24 <210> 352 <211> 26 <212> DNA <213> Human papillomavirus type 59 <220> <221> -35_signal (222) (1) .. (26) <223> 1306-1331 <400> 352 ggtaaccgtg gagaatactg gaaatg 26 <210> 353 <211> 26 <212> DNA <213> Human papillomavirus type 59 <220> <221> -35_signal (222) (1) .. (26) <223> 1372-1397 <400> 353 ctgtagcgac agcagtaaca tggatg 26 <210> 354 <211> 24 <212> DNA <213> Human papillomavirus type 59 <220> <221> -35_signal (222) (1) .. (24) <223> 1421-1444 <400> 354 cccactaatc aattgttaca gtta 24 <210> 355 <211> 22 <212> DNA <213> Human papillomavirus type 59 <220> <221> -35_signal (222) (1) .. (22) <223> 1499-1520 <400> 355 gggttatcat ttcaagattt gg 22 <210> 356 <211> 15 <212> DNA <213> Human papillomavirus type 61 <220> <221> -35_signal (222) (1) .. (15) <223> 6803-6817 <400> 356 ctgctacatc ccccc 15 <210> 357 <211> 20 <212> DNA <213> Human papillomavirus type 61 <220> <221> -35_signal (222) (1) .. (20) <223> 6808-6827 <400> 357 acatcccccc ctgtatctga 20 <210> 358 <211> 20 <212> DNA <213> Human papillomavirus type 61 <220> <221> -35_signal (222) (1) .. (20) <223> 6809-6828 <400> 358 catccccccc tgtatctgaa 20 <210> 359 <211> 22 <212> DNA <213> Human papillomavirus type 61 <220> <221> -35_signal (222) (1) .. (22) <223> 6815-6836 <400> 359 cccctgtatc tgaatataaa gc 22 <210> 360 <211> 20 <212> DNA <213> Human papillomavirus type 61 <220> <221> -35_signal (222) (1) .. (20) <223> 6824-6843 <400> 360 ctgaatataa agccacaagc 20 <210> 361 <211> 23 <212> DNA <213> Human papillomavirus type 61 <220> <221> -35_signal (222) (1) .. (23) <223> 6903-6925 <400> 361 taaaatacat ttaacccctg aaa 23 <210> 362 <211> 19 <212> DNA <213> Human papillomavirus type 61 <220> <221> -35_signal (222) (1) .. (19) <223> 6951-6969 <400> 362 taaggccttg ttggatgac 19 <210> 363 <211> 26 <212> DNA <213> Human papillomavirus type 61 <220> <221> -35_signal (222) (1) .. (26) <223> 6981-7006 <400> 363 tgtggtacca ccaccctcta ccagtt 26 <210> 364 <211> 22 <212> DNA <213> Human papillomavirus type 61 <220> <221> -35_signal (222) (1) .. (22) <223> 7017-7038 <400> 364 atataggttt ttgcagtcca ga 22 <210> 365 <211> 22 <212> DNA <213> Human papillomavirus type 61 <220> <221> -35_signal (222) (1) .. (22) <223> 7056-7077 <400> 365 gggtgctgct gccccgccgc cc 22 <210> 366 <211> 16 <212> DNA <213> Human papillomavirus type 61 <220> <221> -35_signal (222) (1) .. (16) <223> 7089-7104 <400> 366 ctatgccaag ttatcc 16 <210> 367 <211> 23 <212> DNA <213> Human papillomavirus type 61 <220> <221> -35_signal (222) (1) .. (23) <223> 1038-1060 <400> 367 tgcacaggat gacgctgcaa cgg 23 <210> 368 <211> 26 <212> DNA <213> Human papillomavirus type 61 <220> <221> -35_signal (222) (1) .. (26) <223> 1115-1140 <400> 368 ccttggtgga cagtgaatta agtccc 26 <210> 369 <211> 30 <212> DNA <213> Human papillomavirus type 61 <220> <221> -35_signal (222) (1) .. (30) <223> 1168-1197 <400> 369 ggacaggaca gggctaggag aaggctgttt 30 <210> 370 <211> 20 <212> DNA <213> Human papillomavirus type 61 <220> <221> -35_signal (222) (1) .. (20) <223> 1193-1212 <400> 370 tgtttgagca agatagtggc 20 <210> 371 <211> 26 <212> DNA <213> Human papillomavirus type 61 <220> <221> -35_signal (222) (1) .. (26) <223> 1263-1288 <400> 371 ggatgcgcaa catgaggggg gggggg 26 <210> 372 <211> 26 <212> DNA <213> Human papillomavirus type 61 <220> <221> -35_signal (222) (1) .. (26) <223> 1335-1360 <400> 372 ggccgaggcc acaggtaacc aggaaa 26 <210> 373 <211> 26 <212> DNA <213> Human papillomavirus type 61 <220> <221> -35_signal (222) (1) .. (26) <223> 1380-1405 <400> 373 ggcagacata ttagaggtgt ttaagg 26 <210> 374 <211> 26 <212> DNA <213> Human papillomavirus type 61 <220> <221> -35_signal (222) (1) .. (26) <223> 1429-1454 <400> 374 ctgtacaaat tcaaggacct atttgg 26 <210> 375 <211> 21 <212> DNA <213> Human papillomavirus type 61 <220> <221> -35_signal (222) (1) .. (21) <223> 1456-1476 <400> 375 ctagcatttg gggagctggt a 21 <210> 376 <211> 15 <212> DNA <213> Human papillomavirus type 62 <220> <221> -35_signal (222) (1) .. (15) <223> 92-106 <400> 376 ccgcctccac tgctg 15 <210> 377 <211> 20 <212> DNA <213> Human papillomavirus type 62 <220> <221> -35_signal (222) (1) .. (20) <223> 94-113 <400> 377 gcctccactg ctgcagcaga 20 <210> 378 <211> 20 <212> DNA <213> Human papillomavirus type 62 <220> <221> -35_signal (222) (1) .. (20) <223> 101-120 <400> 378 ctgctgcagc agaatacacg 20 <210> 379 <211> 20 <212> DNA <213> Human papillomavirus type 62 <220> <221> -35_signal (222) (1) .. (20) <223> 109-128 <400> 379 gcagaataca cggctaccaa 20 <210> 380 <211> 20 <212> DNA <213> Human papillomavirus type 62 <220> <221> -35_signal (222) (1) .. (20) <223> 110-129 <400> 380 cagaatacac ggctaccaac 20 <210> 381 <211> 23 <212> DNA <213> Human papillomavirus type 62 <220> <221> -35_signal (222) (1) .. (23) <223> 189-211 <400> 381 caaaatacag ttaacccccg aaa 23 <210> 382 <211> 19 <212> DNA <213> Human papillomavirus type 62 <220> <221> -35_signal (222) (1) .. (19) <223> 237-255 <400> 382 caaggacctt ttggatgac 19 <210> 383 <211> 26 <212> DNA <213> Human papillomavirus type 62 <220> <221> -35_signal (222) (1) .. (26) <223> 267-292 <400> 383 ggttttacct cccccttcca ctagtt 26 <210> 384 <211> 22 <212> DNA <213> Human papillomavirus type 62 <220> <221> -35_signal (222) (1) .. (22) <223> 303-324 <400> 384 atatcactat ttcgagtctc gg 22 <210> 385 <211> 19 <212> DNA <213> Human papillomavirus type 62 <220> <221> -35_signal (222) (1) .. (19) <223> 342-360 <400> 385 ggggctgcct acccgtccc 19 <210> 386 <211> 16 <212> DNA <213> Human papillomavirus type 62 <220> <221> -35_signal (222) (1) .. (16) <223> 372-387 <400> 386 gtatgcgcaa atgaca 16 <210> 387 <211> 15 <212> DNA <213> Human papillomavirus type 66 <220> <221> -35_signal (222) (1) .. (15) <223> 6680-6694 <400> 387 cagctaaaag cacat 15 <210> 388 <211> 20 <212> DNA <213> Human papillomavirus type 66 <220> <221> -35_signal (222) (1) .. (20) <223> 6680-6699 <400> 388 cagctaaaag cacattaact 20 <210> 389 <211> 20 <212> DNA <213> Human papillomavirus type 66 <220> <221> -35_signal (222) (1) .. (20) <223> 6683-6702 <400> 389 ctaaaagcac attaactaaa 20 <210> 390 <211> 20 <212> DNA <213> Human papillomavirus type 66 <220> <221> -35_signal (222) (1) .. (20) <223> 6694-6713 <400> 390 ttaactaaat atgatgcccg 20 <210> 391 <211> 20 <212> DNA <213> Human papillomavirus type 66 <220> <221> -35_signal (222) (1) .. (20) <223> 6698-6717 <400> 391 ctaaatatga tgcccgtgaa 20 <210> 392 <211> 23 <212> DNA <213> Human papillomavirus type 66 <220> <221> -35_signal (222) (1) .. (23) <223> 6777-6799 <400> 392 taaaataacc ttaactgcag aag 23 <210> 393 <211> 19 <212> DNA <213> Human papillomavirus type 66 <220> <221> -35_signal (222) (1) .. (19) <223> 6825-6843 <400> 393 taatacttta ttagacgat 19 <210> 394 <211> 26 <212> DNA <213> Human papillomavirus type 66 <220> <221> -35_signal (222) (1) .. (26) <223> 6855-6880 <400> 394 cttatcccca ccagttgcaa ctagct 26 <210> 395 <211> 22 <212> DNA <213> Human papillomavirus type 66 <220> <221> -35_signal (222) (1) .. (22) <223> 6891-6912 <400> 395 atataggtat attaaaagca ca 22 <210> 396 <211> 19 <212> DNA <213> Human papillomavirus type 66 <220> <221> -35_signal (222) (1) .. (19) <223> 6930-6948 <400> 396 ggaacagccc cctgcagaa 19 <210> 397 <211> 16 <212> DNA <213> Human papillomavirus type 66 <220> <221> -35_signal (222) (1) .. (16) <223> 6960-6975 <400> 397 cctggctaaa tataag 16 <210> 398 <211> 22 <212> DNA <213> Human papillomavirus type 66 <220> <221> -35_signal (222) (1) .. (22) <223> 1116-1137 <400> 398 agcacatgca gatgcacaga cg 22 <210> 399 <211> 26 <212> DNA <213> Human papillomavirus type 66 <220> <221> -35_signal (222) (1) .. (26) <223> 1165-1190 <400> 399 ggtagtccct taagtgatat tagtaa 26 <210> 400 <211> 26 <212> DNA <213> Human papillomavirus type 66 <220> <221> -35_signal (222) (1) .. (26) <223> 1194-1219 <400> 400 gcaaactgtg taccgagagg aagtaa 26 <210> 401 <211> 30 <212> DNA <213> Human papillomavirus type 66 <220> <221> -35_signal (222) (1) .. (30) <223> 1224-1253 <400> 401 aaggctaata ttatcagaag acagcgggta 30 <210> 402 <211> 25 <212> DNA <213> Human papillomavirus type 66 <220> <221> -35_signal (222) (1) .. (25) <223> 1276-1300 <400> 402 gaaacatcac aacaggtaga atacg 25 <210> 403 <211> 26 <212> DNA <213> Human papillomavirus type 66 <220> <221> -35_signal (222) (1) .. (26) <223> 1320-1345 <400> 403 gagctcacaa aatggaggct cgcaaa 26 <210> 404 <211> 26 <212> DNA <213> Human papillomavirus type 66 <220> <221> -35_signal (222) (1) .. (26) <223> 1371-1396 <400> 404 atcaaatatg gatatagata caaata 26 <210> 405 <211> 26 <212> DNA <213> Human papillomavirus type 66 <220> <221> -35_signal (222) (1) .. (26) <223> 1413-1438 <400> 405 ccaattgcag gaactattta aaagta 26 <210> 406 <211> 26 <212> DNA <213> Human papillomavirus type 66 <220> <221> -35_signal (222) (1) .. (26) <223> 1447-1472 <400> 406 caaggaagat tacattttaa atttaa 26 <210> 407 <211> 20 <212> DNA <213> Human papillomavirus type 66 <220> <221> -35_signal (222) (1) .. (20) <223> 1473-1492 <400> 407 agaagtgtat ggagtgccat 20 <210> 408 <211> 15 <212> DNA <213> Human papillomavirus type 67 <220> <221> -35_signal (222) (1) .. (15) <223> 6655-6669 <400> 408 ctgaggaaaa atcag 15 <210> 409 <211> 20 <212> DNA <213> Human papillomavirus type 67 <220> <221> -35_signal (222) (1) .. (20) <223> 6657-6676 <400> 409 gaggaaaaat cagaggctac 20 <210> 410 <211> 23 <212> DNA <213> Human papillomavirus type 67 <220> <221> -35_signal (222) (1) .. (23) <223> 6665-6687 <400> 410 atcagaggct acatacaaaa atg 23 <210> 411 <211> 20 <212> DNA <213> Human papillomavirus type 67 <220> <221> -35_signal (222) (1) .. (20) <223> 6658-6677 <400> 411 aggaaaaatc agaggctaca 20 <210> 412 <211> 20 <212> DNA <213> Human papillomavirus type 67 <220> <221> -35_signal (222) (1) .. (20) <223> 6673-6692 <400> 412 ctacatacaa aaatgaaaac 20 <210> 413 <211> 23 <212> DNA <213> Human papillomavirus type 67 <220> <221> -35_signal (222) (1) .. (23) <223> 6752-6774 <400> 413 caaaatatcc cttactgcaa atg 23 <210> 414 <211> 19 <212> DNA <213> Human papillomavirus type 67 <220> <221> -35_signal (222) (1) .. (19) <223> 6800-6818 <400> 414 tccagatata ttagaggac 19 <210> 415 <211> 26 <212> DNA <213> Human papillomavirus type 67 <220> <221> -35_signal (222) (1) .. (26) <223> 6830-6855 <400> 415 ccttacacca cctccttcag gtaatt 26 <210> 416 <211> 22 <212> DNA <213> Human papillomavirus type 67 <220> <221> -35_signal (222) (1) .. (22) <223> 6866-6887 <400> 416 atatagattt gttacctcgc ag 22 <210> 417 <211> 19 <212> DNA <213> Human papillomavirus type 67 <220> <221> -35_signal (222) (1) .. (19) <223> 6905-6923 <400> 417 aacatcccct ccaacagca 19 <210> 418 <211> 16 <212> DNA <213> Human papillomavirus type 67 <220> <221> -35_signal (222) (1) .. (16) <223> 6935-6950 <400> 418 tcttaaaaag tacagt 16 <210> 419 <211> 23 <212> DNA <213> Human papillomavirus type 67 <220> <221> -35_signal (222) (1) .. (23) <223> 1096-1118 <400> 419 agaggaggat gacctaaacg ctg 23 <210> 420 <211> 26 <212> DNA <213> Human papillomavirus type 67 <220> <221> -35_signal (222) (1) .. (26) <223> 1151-1176 <400> 420 caggcatgtg gtggtaatag taatgg 26 <210> 421 <211> 30 <212> DNA <213> Human papillomavirus type 67 <220> <221> -35_signal (222) (1) .. (30) <223> 1199-1228 <400> 421 gccgcaaaac gcagagcata cgacatagaa 30 <210> 422 <211> 26 <212> DNA <213> Human papillomavirus type 67 <220> <221> -35_signal (222) (1) .. (26) <223> 1287-1312 <400> 422 ggcaaaatgg cgatatgcag tgcagt 26 <210> 423 <211> 26 <212> DNA <213> Human papillomavirus type 67 <220> <221> -35_signal (222) (1) .. (26) <223> 1331-1356 <400> 423 gcaagtagta cgggaaacag tgtaga 26 <210> 424 <211> 26 <212> DNA <213> Human papillomavirus type 67 <220> <221> -35_signal (222) (1) .. (26) <223> 1380-1405 <400> 424 gtcaggaaca aagcatgcca ttgcaa 26 <210> 425 <211> 25 <212> DNA <213> Human papillomavirus type 67 <220> <221> -35_signal (222) (1) .. (25) <223> 1423-1447 <400> 425 gcatgtaaat aatataaagg caacg 25 <210> 426 <211> 26 <212> DNA <213> Human papillomavirus type 67 <220> <221> -35_signal (222) (1) .. (26) <223> 1465-1490 <400> 426 ggaagcatat ggggtaacgt ttacac 26 <210> 427 <211> 15 <212> DNA <213> Human papillomavirus type 68 <220> <221> -35_signal (222) (1) .. (15) <223> 2653-2667 <400> 427 ctactactga atcag 15 <210> 428 <211> 20 <212> DNA <213> Human papillomavirus type 68 <220> <221> -35_signal (222) (1) .. (20) <223> 2660-2679 <400> 428 tgaatcagct gtaccaaata 20 <210> 429 <211> 20 <212> DNA <213> Human papillomavirus type 68 <220> <221> -35_signal (222) (1) .. (20) <223> 2661-2680 <400> 429 gaatcagctg taccaaatat 20 <210> 430 <211> 22 <212> DNA <213> Human papillomavirus type 68 <220> <221> -35_signal (222) (1) .. (22) <223> 2665-2686 <400> 430 cagctgtacc aaatatttat ga 22 <210> 431 <211> 20 <212> DNA <213> Human papillomavirus type 68 <220> <221> -35_signal (222) (1) .. (20) <223> 2677-2696 <400> 431 atatttatga tcctaataaa 20 <210> 432 <211> 19 <212> DNA <213> Human papillomavirus type 68 <220> <221> -35_signal (222) (1) .. (19) <223> 2804-2822 <400> 432 tcctgctatt ttggatgat 19 <210> 433 <211> 23 <212> DNA <213> Human papillomavirus type 68 <220> <221> -35_signal (222) (1) .. (23) <223> 2756-2778 <400> 433 tactataaca ttgtccactg atg 23 <210> 434 <211> 26 <212> DNA <213> Human papillomavirus type 68 <220> <221> -35_signal (222) (1) .. (26) <223> 2834-2859 <400> 434 tgttgcccct ccaccatctg ctagtc 26 <210> 435 <211> 22 <212> DNA <213> Human papillomavirus type 68 <220> <221> -35_signal (222) (1) .. (22) <223> 2870-2891 <400> 435 ataccgctat ctgcaatcag ca 22 <210> 436 <211> 19 <212> DNA <213> Human papillomavirus type 68 <220> <221> -35_signal (222) (1) .. (19) <223> 2909-2927 <400> 436 agacgcccct gcacctact 19 <210> 437 <211> 16 <212> DNA <213> Human papillomavirus type 68 <220> <221> -35_signal (222) (1) .. (16) <223> 2939-2954 <400> 437 atatgatggc ttaaac 16 <210> 438 <211> 23 <212> DNA <213> Human papillomavirus type 68 <220> <221> -35_signal (222) (1) .. (23) <223> 4990-5012 <400> 438 ggcccaaagg gatgcacaaa cag 23 <210> 439 <211> 26 <212> DNA <213> Human papillomavirus type 68 <220> <221> -35_signal (222) (1) .. (26) <223> 5055-5080 <400> 439 gccctttagc aaagtcgcca ttacag 26 <210> 440 <211> 20 <212> DNA <213> Human papillomavirus type 68 <220> <221> -35_signal (222) (1) .. (20) <223> 5115-5134 <400> 440 ggtaactgta gcaactaata 20 <210> 441 <211> 30 <212> DNA <213> Human papillomavirus type 68 <220> <221> -35_signal (222) (1) .. (30) <223> 5163-5192 <400> 441 ggaaaatggc gacagcatac gggaggactg 30 <210> 442 <211> 26 <212> DNA <213> Human papillomavirus type 68 <220> <221> -35_signal (222) (1) .. (26) <223> 5203-5228 <400> 442 gacagtgcta tagatagtga aaacca 26 <210> 443 <211> 24 <212> DNA <213> Human papillomavirus type 68 <220> <221> -35_signal (222) (1) .. (24) <223> 5242-5265 <400> 443 cctactacgc aactaaaagt atta 24 <210> 444 <211> 26 <212> DNA <213> Human papillomavirus type 68 <220> <221> -35_signal (222) (1) .. (26) <223> 5285-5310 <400> 444 aagctgcaat gttaacagaa tttaaa 26 <210> 445 <211> 20 <212> DNA <213> Human papillomavirus type 69 <220> <221> -35_signal (222) (1) .. (20) <223> 6572-6591 <400> 445 tattagtact gtatctgcac 20 <210> 446 <211> 15 <212> DNA <213> Human papillomavirus type 69 <220> <221> -35_signal (222) (1) .. (15) <223> 6580-6594 <400> 446 ctgtatctgc acaat 15 <210> 447 <211> 20 <212> DNA <213> Human papillomavirus type 69 <220> <221> -35_signal (222) (1) .. (20) <223> 6580-6599 <400> 447 ctgtatctgc acaatctgca 20 <210> 448 <211> 20 <212> DNA <213> Human papillomavirus type 69 <220> <221> -35_signal (222) (1) .. (20) <223> 6587-6606 <400> 448 tgcacaatct gcatctgcca 20 <210> 449 <211> 22 <212> DNA <213> Human papillomavirus type 69 <220> <221> -35_signal (222) (1) .. (22) <223> 6591-6612 <400> 449 caatctgcat ctgccacttt ta 22 <210> 450 <211> 20 <212> DNA <213> Human papillomavirus type 69 <400> 450 ccacttttaa accatcagat 20 <210> 451 <211> 23 <212> DNA <213> Human papillomavirus type 69 <220> <221> -35_signal (222) (1) .. (23) <223> 6683-6705 <400> 451 taaaattact cttaccactg atg 23 <210> 452 <211> 19 <212> DNA <213> Human papillomavirus type 69 <220> <221> -35_signal (222) (1) .. (19) <223> 6731-6749 <400> 452 ttctactatt ttggaaaat 19 <210> 453 <211> 26 <212> DNA <213> Human papillomavirus type 69 <220> <221> -35_signal (222) (1) .. (26) <223> 6761-6786 <400> 453 ccttaccttg cctcctactg ctagtt 26 <210> 454 <211> 22 <212> DNA <213> Human papillomavirus type 69 <220> <221> -35_signal (222) (1) .. (22) <223> 6797-6818 <400> 454 atataggttt attaaaaatt ca 22 <210> 455 <211> 19 <212> DNA <213> Human papillomavirus type 69 <220> <221> -35_signal (222) (1) .. (19) <223> 6836-6854 <400> 455 cgatgcccct gcacagccc 19 <210> 456 <211> 23 <212> DNA <213> Human papillomavirus type 69 <220> <221> -35_signal (222) (1) .. (23) <223> 1101-1123 <400> 456 aacacaagca aataagaagg cag 23 <210> 457 <211> 26 <212> DNA <213> Human papillomavirus type 69 <220> <221> -35_signal (222) (1) .. (26) <223> 1158-1183 <400> 457 gaacagcccg ttgcaagaca taacaa 26 <210> 458 <211> 25 <212> DNA <213> Human papillomavirus type 69 <220> <221> -35_signal (222) (1) .. (25) <223> 1208-1232 <400> 458 cagacgaagt aaacaattta caggc 25 <210> 459 <211> 30 <212> DNA <213> Human papillomavirus type 69 <220> <221> -35_signal (222) (1) .. (30) <223> 1238-1267 <400> 459 ggagagcagt ggacagtgtt ccggacagcg 30 <210> 460 <211> 26 <212> DNA <213> Human papillomavirus type 69 <220> <221> -35_signal (222) (1) .. (26) <223> 1308-1333 <400> 460 ggtagataaa cacaatgaac aaaatg 26 <210> 461 <211> 22 <212> DNA <213> Human papillomavirus type 69 <220> <221> -35_signal (222) (1) .. (22) <223> 1360-1381 <400> 461 tcaagtggat ctgtatcaga ca 22 <210> 462 <211> 25 <212> DNA <213> Human papillomavirus type 69 <220> <221> -35_signal (222) (1) .. (25) <223> 1399-1423 <400> 462 gcacaggcaa gtagtgtaac caaaa 25 <210> 463 <211> 24 <212> DNA <213> Human papillomavirus type 69 <220> <221> -35_signal (222) (1) .. (24) <223> 1445-1468 <400> 463 gtaatgtaaa agcagcatta ttaa 24 <210> 464 <211> 21 <212> DNA <213> Human papillomavirus type 69 <220> <221> -35_signal (222) (1) .. (21) <223> 1481-1501 <400> 464 cagtatatgg tgtaagttat a 21 <210> 465 <211> 20 <212> DNA <213> Human papillomavirus type 6 <220> <221> -35_signal (222) (1) .. (20) <223> 6814-6833 <400> 465 catccgtaac tacatcttcc 20 <210> 466 <211> 20 <212> DNA <213> Human papillomavirus type 6 <220> <221> -35_signal (222) (1) .. (20) <223> 6815-6834 <400> 466 atccgtaact acatcttcca 20 <210> 467 <211> 22 <212> DNA <213> Human papillomavirus type 6 <220> <221> -35_signal (222) (1) .. (22) <223> 6823-6844 <400> 467 ctacatcttc cacatacacc aa 22 <210> 468 <211> 20 <212> DNA <213> Human papillomavirus type 6 <220> <221> -35_signal (222) (1) .. (20) <223> 6826-6845 <400> 468 catcttccac atacaccaat 20 <210> 469 <211> 20 <212> DNA <213> Human papillomavirus type 6 <220> <221> -35_signal (222) (1) .. (20) <223> 6827-6846 <400> 469 atcttccaca tacaccaatt 20 <210> 470 <211> 20 <212> DNA <213> Human papillomavirus type 6 <220> <221> -35_signal (222) (1) .. (20) <223> 6832-6851 <400> 470 ccacatacac caattctgat 20 <210> 471 <211> 23 <212> DNA <213> Human papillomavirus type 6 <220> <221> -35_signal (222) (1) .. (23) <223> 6911-6933 <400> 471 tagcattaca ttgtctgctg aag 23 <210> 472 <211> 19 <212> DNA <213> Human papillomavirus type 6 <220> <221> -35_signal (222) (1) .. (19) <223> 6959-6977 <400> 472 tccctctgtt ttggaagac 19 <210> 473 <211> 26 <212> DNA <213> Human papillomavirus type 6 <220> <221> -35_signal (222) (1) .. (26) <223> 6989-7014 <400> 473 gttatcgcct cccccaaatg gtacat 26 <210> 474 <211> 22 <212> DNA <213> Human papillomavirus type 6 <220> <221> -35_signal (222) (1) .. (22) <223> 7025-7046 <400> 474 ctataggtat gtgcagtcac ag 22 <210> 475 <211> 19 <212> DNA <213> Human papillomavirus type 6 <220> <221> -35_signal (222) (1) .. (19) <223> 7064-7082 <400> 475 gcccactcct gaaaaggaa 19 <210> 476 <211> 16 <212> DNA <213> Human papillomavirus type 6 <220> <221> -35_signal (222) (1) .. (16) <223> 7094-7109 <400> 476 ctataagaac cttagt 16 <210> 477 <211> 23 <212> DNA <213> Human papillomavirus type 6 <220> <221> -35_signal (222) (1) .. (23) <223> 1045-1067 <400> 477 ggcggacacc cattatgcga ctg 23 <210> 478 <211> 26 <212> DNA <213> Human papillomavirus type 6 <220> <221> -35_signal (222) (1) .. (26) <223> 1106-1131 <400> 478 gttagtccta taaacactat agccga 26 <210> 479 <211> 30 <212> DNA <213> Human papillomavirus type 6 <220> <221> -35_signal (222) (1) .. (30) <223> 1154-1183 <400> 479 ccacgattgg acgccattaa acttacaaga 30 <210> 480 <211> 26 <212> DNA <213> Human papillomavirus type 6 <220> <221> -35_signal (222) (1) .. (26) <223> 1206-1231 <400> 480 ggctgtttca aaccagggaa ctaacg 26 <210> 481 <211> 26 <212> DNA <213> Human papillomavirus type 6 <220> <221> -35_signal (222) (1) .. (26) <223> 1279-1304 <400> 481 ggtagagaaa catggcgtac cggaaa 26 <210> 482 <211> 26 <212> DNA <213> Human papillomavirus type 6 <220> <221> -35_signal (222) (1) .. (26) <223> 1327-1352 <400> 482 ggacacagga agggacatag aggggg 26 <210> 483 <211> 26 <212> DNA <213> Human papillomavirus type 6 <220> <221> -35_signal (222) (1) .. (26) <223> 1378-1403 <400> 483 cacaaacagt gtacgggagc atgcag 26 <210> 484 <211> 26 <212> DNA <213> Human papillomavirus type 6 <220> <221> -35_signal (222) (1) .. (26) <223> 1426-1451 <400> 484 gctaaaatgt aaagatttac gggcag 26 <210> 485 <211> 26 <212> DNA <213> Human papillomavirus type 6 <220> <221> -35_signal (222) (1) .. (26) <223> 1476-1501 <400> 485 gctttgggct gtcttttata gattta 26 <210> 486 <211> 20 <212> DNA <213> Human papillomavirus type 70 <220> <221> -35_signal (222) (1) .. (20) <223> 6614-6633 <400> 486 tgtctgcctg caccgaaacg 20 <210> 487 <211> 15 <212> DNA <213> Human papillomavirus type 70 <220> <221> -35_signal (222) (1) .. (15) <223> 6621-6635 <400> 487 ctgcaccgaa acggc 15 <210> 488 <211> 20 <212> DNA <213> Human papillomavirus type 70 <220> <221> -35_signal (222) (1) .. (20) <223> 6628-6647 <400> 488 gaaacggcca tacctgctgt 20 <210> 489 <211> 20 <212> DNA <213> Human papillomavirus type 70 <220> <221> -35_signal (222) (1) .. (20) <223> 6627-6646 <400> 489 cgaaacggcc atacctgctg 20 <210> 490 <211> 22 <212> DNA <213> Human papillomavirus type 70 <220> <221> -35_signal (222) (1) .. (22) <223> 6632-6653 <400> 490 cggccatacc tgctgtatat ag 22 <210> 491 <211> 20 <212> DNA <213> Human papillomavirus type 70 <220> <221> -35_signal (222) (1) .. (20) <223> 6644-6663 <400> 491 ctgtatatag ccctacaaag 20 <210> 492 <211> 23 <212> DNA <213> Human papillomavirus type 70 <220> <221> -35_signal (222) (1) .. (23) <223> 6723-6745 <400> 492 tactatcaca ttaactgctg acg 23 <210> 493 <211> 19 <212> DNA <213> Human papillomavirus type 70 <220> <221> -35_signal (222) (1) .. (19) <223> 6771-6789 <400> 493 tcctgcaatt ttggacaat 19 <210> 494 <211> 26 <212> DNA <213> Human papillomavirus type 70 <220> <221> -35_signal (222) (1) .. (26) <223> 6801-6826 <400> 494 agttacccct ccaccatctg caagct 26 <210> 495 <211> 22 <212> DNA <213> Human papillomavirus type 70 <220> <221> -35_signal (222) (1) .. (22) <223> 6837-6858 <400> 495 gtataggtat ttacaatcag ca 22 <210> 496 <211> 19 <212> DNA <213> Human papillomavirus type 70 <220> <221> -35_signal (222) (1) .. (19) <223> 6876-6894 <400> 496 ggatgctcct acacctgaa 19 <210> 497 <211> 16 <212> DNA <213> Human papillomavirus type 70 <220> <221> -35_signal (222) (1) .. (16) <223> 6906-6921 <400> 497 ctatgacgat ttaaaa 16 <210> 498 <211> 23 <212> DNA <213> Human papillomavirus type 70 <220> <221> -35_signal (222) (1) .. (23) <223> 1149-1171 <400> 498 ggcccaaagg gatgcacaat cag 23 <210> 499 <211> 23 <212> DNA <213> Human papillomavirus type 70 <220> <221> -35_signal (222) (1) .. (23) <223> 1202-1224 <400> 499 gcaatctaaa taaaagtcct tgt 23 <210> 500 <211> 25 <212> DNA <213> Human papillomavirus type 70 <220> <221> -35_signal (222) (1) .. (25) <223> 1240-1264 <400> 500 gtacataggg aacaaagggt aacac 25 <210> 501 <211> 23 <212> DNA <213> Human papillomavirus type 70 <220> <221> -35_signal (222) (1) .. (23) <223> 1278-1300 <400> 501 ggtaaacata tgcaataaac agg 23 <210> 502 <211> 30 <212> DNA <213> Human papillomavirus type 70 <220> <221> -35_signal (222) (1) .. (30) <223> 1306-1335 <400> 502 acaaacgtgt attcagtacc agacagcggc 30 <210> 503 <211> 26 <212> DNA <213> Human papillomavirus type 70 <220> <221> -35_signal (222) (1) .. (26) <223> 1378-1403 <400> 503 gtagtaaata atacaaatgg ggaaga 26 <210> 504 <211> 26 <212> DNA <213> Human papillomavirus type 70 <220> <221> -35_signal (222) (1) .. (26) <223> 1446-1471 <400> 504 ggagtgcagt agtgtagaca gtgcta 26 <210> 505 <211> 26 <212> DNA <213> Human papillomavirus type 70 <220> <221> -35_signal (222) (1) .. (26) <223> 1491-1516 <400> 505 tccacagtca cctactgcac agctaa 26 <210> 506 <211> 25 <212> DNA <213> Human papillomavirus type 70 <220> <221> -35_signal (222) (1) .. (20) <223> 1531-1555 <400> 506 gctaataacc aaaaagccat actac 25 <210> 507 <211> 26 <212> DNA <213> Human papillomavirus type 70 <220> <221> -35_signal (222) (1) .. (26) <223> 1570-1595 <400> 507 cacacatatg gattagcatt taacga 26 <210> 508 <211> 20 <212> DNA <213> Human papillomavirus type 72 <220> <221> -35_signal (222) (1) .. (20) <223> 6759-6778 <400> 508 atctgttggt ttaatgagct 20 <210> 509 <211> 20 <212> DNA <213> Human papillomavirus type 72 <220> <221> -35_signal (222) (1) .. (20) <223> 6780-6799 <400> 509 tttgtgacag ttgtagatac 20 <210> 510 <211> 15 <212> DNA <213> Human papillomavirus type 72 <220> <221> -35_signal (222) (1) .. (15) <223> 6829-6843 <400> 510 ctgccacagc gtcct 15 <210> 511 <211> 20 <212> DNA <213> Human papillomavirus type 72 <220> <221> -35_signal (222) (1) .. (20) <223> 6834-6853 <400> 511 acagcgtcct ctgtatcaga 20 <210> 512 <211> 20 <212> DNA <213> Human papillomavirus type 72 <220> <221> -35_signal (222) (1) .. (20) <223> 6832-6851 <400> 512 ccacagcgtc ctctgtatca 20 <210> 513 <211> 22 <212> DNA <213> Human papillomavirus type 72 <220> <221> -35_signal (222) (1) .. (22) <223> 6836-6857 <400> 513 agcgtcctct gtatcagaat at 22 <210> 514 <211> 20 <212> DNA <213> Human papillomavirus type 72 <220> <221> -35_signal (222) (1) .. (20) <223> 6850-6869 <400> 514 cagaatatac agcttctaat 20 <210> 515 <211> 23 <212> DNA <213> Human papillomavirus type 72 <220> <221> -35_signal (222) (1) .. (23) <223> 6929-6951 <400> 515 taaaattcac ttaactcctg aaa 23 <210> 516 <211> 19 <212> DNA <213> Human papillomavirus type 72 <220> <221> -35_signal (222) (1) .. (19) <223> 6977-6995 <400> 516 taaggcctta ttggatgac 19 <210> 517 <211> 26 <212> DNA <213> Human papillomavirus type 72 <220> <221> -35_signal (222) (1) .. (26) <223> 7007-7032 <400> 517 tgtggtgcct cctccttcta ccagtt 26 <210> 518 <211> 22 <212> DNA <213> Human papillomavirus type 72 <220> <221> -35_signal (222) (1) .. (22) <223> 7043-7064 <400> 518 ctataggttt ttgcagtctc gt 22 <210> 519 <211> 22 <212> DNA <213> Human papillomavirus type 72 <220> <221> -35_signal (222) (1) .. (22) <223> 7082-7103 <400> 519 gggggctgcc acccctcctc ct 22 <210> 520 <211> 16 <212> DNA <213> Human papillomavirus type 72 <220> <221> -35_signal (222) (1) .. (16) <223> 7115-7130 <400> 520 atatgctaac ttatcc 16 <210> 521 <211> 15 <212> DNA <213> Human papillomavirus type 74 <220> <221> -35_signal (222) (1) .. (15) <223> 1686-1700 <400> 521 cctacctcac aatcg 15 <210> 522 <211> 20 <212> DNA <213> Human papillomavirus type 74 <400> 522 ctcacaatcg ccttctgcta 20 <210> 523 <211> 20 <212> DNA <213> Human papillomavirus type 74 <220> <221> -35_signal (222) (1) .. (20) <223> 1689-1708 <400> 523 acctcacaat cgccttctgc 20 <210> 524 <211> 22 <212> DNA <213> Human papillomavirus type 74 <220> <221> -35_signal (222) (1) .. (22) <223> 1695-1716 <400> 524 caatcgcctt ctgctacata ta 22 <210> 525 <211> 22 <212> DNA <213> Human papillomavirus type 74 <220> <221> -35_signal (222) (1) .. (22) <223> 1694-1715 <400> 525 acaatcgcct tctgctacat at 22 <210> 526 <211> 20 <212> DNA <213> Human papillomavirus type 74 <220> <221> -35_signal (222) (1) .. (20) <223> 1708-1727 <400> 526 ctacatataa tagttcagac 20 <210> 527 <211> 23 <212> DNA <213> Human papillomavirus type 74 <220> <221> -35_signal (222) (1) .. (23) <223> 1787-1809 <400> 527 tagtattaag ttaactgctg agg 23 <210> 528 <211> 19 <212> DNA <213> Human papillomavirus type 74 <220> <221> -35_signal (222) (1) .. (19) <223> 1835-1853 <400> 528 tcctacagtt ttagaagag 19 <210> 529 <211> 26 <212> DNA <213> Human papillomavirus type 74 <220> <221> -35_signal (222) (1) .. (26) <223> 1865-1890 <400> 529 gctaacgcct ccccccaatg gtactt 26 <210> 530 <211> 22 <212> DNA <213> Human papillomavirus type 74 <220> <221> -35_signal (222) (1) .. (22) <223> 1901-1922 <400> 530 ctacagatat gtgcagtccc ag 22 <210> 531 <211> 19 <212> DNA <213> Human papillomavirus type 74 <220> <221> -35_signal (222) (1) .. (19) <223> 1940-1958 <400> 531 acctacgcct gataaagca 19 <210> 532 <211> 16 <212> DNA <213> Human papillomavirus type 74 <400> 532 ctatgcaaat ttaagt 16 <210> 533 <211> 15 <212> DNA <213> Human papillomavirus type 82 <220> <221> -35_signal (222) (1) .. (15) <223> 6608-6622 <400> 533 tgctgttact ccatc 15 <210> 534 <211> 20 <212> DNA <213> Human papillomavirus type 82 <220> <221> -35_signal (222) (1) .. (20) <223> 6608-6627 <400> 534 tgctgttact ccatctgttg 20 <210> 535 <211> 20 <212> DNA <213> Human papillomavirus type 82 <220> <221> -35_signal (222) (1) .. (20) <223> 6615-6634 <400> 535 actccatctg ttgcacaaac 20 <210> 536 <211> 20 <212> DNA <213> Human papillomavirus type 82 <220> <221> -35_signal (222) (1) .. (20) <223> 6631-6650 <400> 536 aaacatttac tccagcaaac 20 <210> 537 <211> 23 <212> DNA <213> Human papillomavirus type 82 <220> <221> -35_signal (222) (1) .. (23) <223> 6710-6732 <400> 537 taaaatcact ttaactactg aaa 23 <210> 538 <211> 19 <212> DNA <213> Human papillomavirus type 82 <220> <221> -35_signal (222) (1) .. (19) <223> 6758-6776 <400> 538 ttctacaatt ttagaacag 19 <210> 539 <211> 26 <212> DNA <213> Human papillomavirus type 82 <220> <221> -35_signal (222) (1) .. (26) <223> 6788-6813 <400> 539 attaacattg cccccctccg ctagtt 26 <540> 540 <211> 22 <212> DNA <213> Human papillomavirus type 82 <220> <221> -35_signal (222) (1) .. (22) <223> 6824-6845 <400> 540 ctatcgattt gtaaaaaatg ca 22 <210> 541 <211> 19 <212> DNA <213> Human papillomavirus type 82 <220> <221> -35_signal (222) (1) .. (19) <223> 6863-6881 <400> 541 ggacagtcct ccacaggct 19 <210> 542 <211> 23 <212> DNA <213> Human papillomavirus type 82 <220> <221> -35_signal (222) (1) .. (23) <223> 1094-1116 <400> 542 aacacaggca cacaaagagg ctg 23 <210> 543 <211> 25 <212> DNA <213> Human papillomavirus type 82 <220> <221> -35_signal (222) (1) .. (25) <223> 1156-1180 <400> 543 gcagcccatt aaaagacatt acaaa 25 <210> 544 <211> 30 <212> DNA <213> Human papillomavirus type 82 <220> <221> -35_signal (222) (1) .. (30) <223> 1201-1230 <400> 544 gtcagcaaca accaaaacag gcaaaccttc 30 <210> 545 <211> 27 <212> DNA <213> Human papillomavirus type 82 <220> <221> -35_signal (222) (1) .. (27) <223> 1243-1269 <400> 545 ggagattact ggacagttat ccggaca 27 <210> 546 <211> 26 <212> DNA <213> Human papillomavirus type 82 <220> <221> -35_signal (222) (1) .. (26) <223> 1309-1334 <400> 546 ccttacaggt agatgggcaa aatgac 26 <210> 547 <211> 26 <212> DNA <213> Human papillomavirus type 82 <220> <221> -35_signal (222) (1) .. (26) <223> 1367-1392 <400> 547 gagcagcgac agaagtacag agatag 26 <210> 548 <211> 25 <212> DNA <213> Human papillomavirus type 82 <220> <221> -35_signal (222) (1) .. (25) <223> 1413-1437 <400> 548 gctaccaatg taggactaaa cagta 25 <210> 549 <211> 26 <212> DNA <213> Human papillomavirus type 82 <220> <221> -35_signal (222) (1) .. (26) <223> 1456-1481 <400> 549 gtagcaatgc aaaagcaatg tttatg 26 <210> 550 <211> 26 <212> DNA <213> Human papillomavirus type 82 <220> <221> -35_signal (222) (1) .. (26) <223> 1503-1528 <400> 550 ggtgttagtt ataatgagtt ggtaag 26 <210> 551 <211> 20 <212> DNA <213> Human papillomavirus type CP8061 <220> <221> -35_signal (222) (1) .. (20) <223> 86-105 <400> 551 tctgtgctac caaaactgtt 20 <210> 552 <211> 15 <212> DNA <213> Human papillomavirus type CP8061 <220> <221> -35_signal (222) (1) .. (15) <223> 92-106 <400> 552 ctaccaaaac tgttg 15 <210> 553 <211> 20 <212> DNA <213> Human papillomavirus type CP8061 <220> <221> -35_signal (222) (1) .. (20) <223> 94-113 <400> 553 accaaaactg ttgagtctac 20 <210> 554 <211> 22 <212> DNA <213> Human papillomavirus type CP8061 <220> <221> -35_signal (222) (1) .. (22) <223> 99-120 <400> 554 aactgttgag tctacatata aa 22 <210> 555 <211> 20 <212> DNA <213> Human papillomavirus type CP8061 <220> <221> -35_signal (222) (1) .. (20) <223> 103-122 <400> 555 gttgagtcta catataaagc 20 <210> 556 <211> 20 <212> DNA <213> Human papillomavirus type CP8061 <220> <221> -35_signal (222) (1) .. (20) <223> 110-129 <400> 556 ctacatataa agcctctagt 20 <210> 557 <211> 23 <212> DNA <213> Human papillomavirus type CP8061 <400> 557 tgttattaat ttaacagctg aaa 23 <210> 558 <211> 19 <212> DNA <213> Human papillomavirus type CP8061 <220> <221> -35_signal (222) (1) .. (19) <223> 237-255 <400> 558 tgctacatta ctggaggac 19 <210> 559 <211> 20 <212> DNA <213> Human papillomavirus type CP8061 <220> <221> -35_signal (222) (1) .. (20) <223> 267-286 <400> 559 gttcttacca cctcctactg 20 <210> 560 <211> 22 <212> DNA <213> Human papillomavirus type CP8061 <220> <221> -35_signal (222) (1) .. (22) <223> 303-324 <400> 560 ctaccgcttt ttacagtctc ag 22 <210> 561 <211> 22 <212> DNA <213> Human papillomavirus type CP8061 <220> <221> -35_signal (222) (1) .. (22) <223> 342-363 <400> 561 aaacagtcct cctcctgcag aa 22 <210> 562 <211> 16 <212> DNA <213> Human papillomavirus type CP8061 <220> <221> -35_signal (222) (1) .. (16) <223> 375-390 <400> 562 ctatgcagat cttaca 16 <210> 563 <211> 15 <212> DNA <213> Human papillomavirus type CP8304 <220> <221> -35_signal (222) (1) .. (15) <223> 92-106 <400> 563 cagctacatc tgctg 15 <210> 564 <211> 20 <212> DNA <213> Human papillomavirus type CP8304 <220> <221> -35_signal (222) (1) .. (20) <223> 94-113 <400> 564 gctacatctg ctgctgcaga 20 <210> 565 <211> 22 <212> DNA <213> Human papillomavirus type CP8304 <220> <221> -35_signal (222) (1) .. (22) <223> 97-118 <400> 565 acatctgctg ctgcagaata ca 22 <210> 566 <211> 20 <212> DNA <213> Human papillomavirus type CP8304 <220> <221> -35_signal (222) (1) .. (20) <223> 105-124 <400> 566 tgctgcagaa tacaaggcct 20 <210> 567 <211> 20 <212> DNA <213> Human papillomavirus type CP8304 <220> <221> -35_signal (222) (1) .. (20) <223> 106-125 <400> 567 gctgcagaat acaaggcctc 20 <210> 568 <211> 20 <212> DNA <213> Human papillomavirus type CP8304 <220> <221> -35_signal (222) (1) .. (20) <223> 110-129 <400> 568 cagaatacaa ggcctctaac 20 <210> 569 <211> 23 <212> DNA <213> Human papillomavirus type CP8304 <220> <221> -35_signal (222) (1) .. (23) <223> 189-211 <400> 569 taaaatacag ttaacaccag aaa 23 <210> 570 <211> 19 <212> DNA <213> Human papillomavirus type CP8304 <220> <221> -35_signal (222) (1) .. (19) <223> 237-255 <400> 570 caaggcactg ttggatgat 19 <210> 571 <211> 26 <212> DNA <213> Human papillomavirus type CP8304 <220> <221> -35_signal (222) (1) .. (26) <223> 267-292 <400> 571 tgtgttgcca cctccttcca ccagtt 26 <210> 572 <211> 22 <212> DNA <213> Human papillomavirus type CP8304 <220> <221> -35_signal (222) (1) .. (22) <223> 303-324 <400> 572 atatcgcttt ttacagtctc gg 22 <210> 573 <211> 22 <212> DNA <213> Human papillomavirus type CP8304 <220> <221> -35_signal (222) (1) .. (22) <223> 342-363 <400> 573 gggtgctgct gcccctgcgc cc 22 <210> 574 <211> 16 <212> DNA <213> Human papillomavirus type CP8304 <220> <221> -35_signal (222) (1) .. (16) <223> 375-390 <400> 574 ttatgccgac atgtca 16 <210> 575 <211> 20 <212> DNA <213> Human papillomavirus type L1AE5 <220> <221> -35_signal (222) (1) .. (20) <223> 69-88 <400> 575 atctactgca actactaatc 20 <210> 576 <211> 15 <212> DNA <213> Human papillomavirus type L1AE5 <220> <221> -35_signal (222) (1) .. (15) <223> 74-88 <400> 576 ctgcaactac taatc 15 <210> 577 <211> 20 <212> DNA <213> Human papillomavirus type LIAE5 <220> <221> -35_signal (222) (1) .. (20) <223> 74-93 <400> 577 ctgcaactac taatccagtt 20 <210> 578 <211> 22 <212> DNA <213> Human papillomavirus type L1AE5 <220> <221> -35_signal (222) (1) .. (22) <223> 79-100 <400> 578 actactaatc cagttccatc ta 22 <210> 579 <211> 20 <212> DNA <213> Human papillomavirus type L1AE5 <220> <221> -35_signal (222) (1) .. (20) <223> 83-102 <400> 579 ctaatccagt tccatctata 20 <210> 580 <211> 20 <212> DNA <213> Human papillomavirus type L1AE5 <220> <221> -35_signal (222) (1) .. (20) <223> 98-117 <400> 580 ctatatatga accttctaaa 20 <210> 581 <211> 23 <212> DNA <213> Human papillomavirus type L1AE5 <220> <221> -35_signal (222) (1) .. (23) <223> 177-199 <400> 581 taaaattaca cttactactg atg 23 <210> 582 <211> 19 <212> DNA <213> Human papillomavirus type L1AE5 <220> <221> -35_signal (222) (1) .. (19) <223> 225-243 <400> 582 tcctactatt ttagatagt 19 <210> 583 <211> 26 <212> DNA <213> Human papillomavirus type L1AE5 <220> <221> -35_signal (222) (1) .. (26) <223> 255-280 <400> 583 tgttagtcct cccccatctg ctagct 26 <210> 584 <211> 22 <212> DNA <213> Human papillomavirus type L1AE5 <220> <221> -35_signal (222) (1) .. (22) <223> 291-312 <400> 584 atataggttt ttacagtcat ct 22 <210> 585 <211> 19 <212> DNA <213> Human papillomavirus type L1AE5 <220> <221> -35_signal (222) (1) .. (19) <223> 330-348 <400> 585 ggatgtggtt gttccacaa 19 <210> 586 <211> 20 <212> DNA <213> Human papillomavirus type MM4 <220> <221> -35_signal (222) (1) .. (20) <223> 92-111 <400> 586 ctgctgttac tcaatctgtt 20 <210> 587 <211> 15 <212> DNA <213> Human papillomavirus type MM4 <220> <221> -35_signal (222) (1) .. (15) <223> 93-107 <400> 587 tgctgttact caatc 15 <210> 588 <211> 20 <212> DNA <213> Human papillomavirus type MM4 <220> <221> -35_signal (222) (1) .. (20) <223> 97-116 <400> 588 gttactcaat ctgttgcaca 20 <210> 589 <211> 20 <212> DNA <213> Human papillomavirus type MM4 <220> <221> -35_signal (222) (1) .. (20) <223> 111-130 <400> 589 tgcacaaaca tttactccag 20 <210> 590 <211> 22 <212> DNA <213> Human papillomavirus type MM4 <220> <221> -35_signal (222) (1) .. (22) <223> 98-119 <400> 590 ttactcaatc tgttgcacaa ac 22 <210> 591 <211> 20 <212> DNA <213> Human papillomavirus type MM4 <220> <221> -35_signal (222) (1) .. (20) <223> 116-135 <400> 591 aaacatttac tccagcaaac 20 <210> 592 <211> 23 <212> DNA <213> Human papillomavirus type MM4 <220> <221> -35_signal (222) (1) .. (23) <223> 195-217 <400> 592 taaaatcact ttaactactg aaa 23 <210> 593 <211> 19 <212> DNA <213> Human papillomavirus type MM4 <220> <221> -35_signal (222) (1) .. (19) <223> 243-261 <400> 593 ttctacaatt ttagaacag 19 <210> 594 <211> 26 <212> DNA <213> Human papillomavirus type MM4 <220> <221> -35_signal (222) (1) .. (26) <223> 273-298 <400> 594 attaaccttg cccccctcag ctagtt 26 <210> 595 <211> 22 <212> DNA <213> Human papillomavirus type MM4 <220> <221> -35_signal (222) (1) .. (22) <223> 309-330 <400> 595 ctatcgattt gtaaaaaatg ca 22 <210> 596 <211> 19 <212> DNA <213> Human papillomavirus type MM4 <220> <221> -35_signal (222) (1) .. (19) <223> 348-366 <400> 596 ggacagtcct ccacaggct 19 <210> 597 <211> 15 <212> DNA <213> Human papillomavirus type MM7 <220> <221> -35_signal (222) (1) .. (15) <223> 93-107 <400> 597 tgctgctaca caggc 15 <210> 598 <211> 20 <212> DNA <213> Human papillomavirus type MM7 <220> <221> -35_signal (222) (1) .. (20) <223> 94-113 <400> 598 gctgctacac aggctaatga 20 <210> 599 <211> 20 <212> DNA <213> Human papillomavirus type MM7 <220> <221> -35_signal (222) (1) .. (20) <223> 96-115 <400> 599 tgctacacag gctaatgaat 20 <210> 600 <211> 22 <212> DNA <213> Human papillomavirus type MM7 <220> <221> -35_signal (222) (1) .. (22) <223> 98-119 <400> 600 ctacacaggc taatgaatac ac 22 <210> 601 <211> 20 <212> DNA <213> Human papillomavirus type MM7 <220> <221> -35_signal (222) (1) .. (20) <223> 110-129 <400> 601 atgaatacac agcctctaac 20 <210> 602 <211> 23 <212> DNA <213> Human papillomavirus type MM7 <220> <221> -35_signal (222) (1) .. (23) <223> 189-211 <400> 602 caaaatacat cttacccctg aaa 23 <210> 603 <211> 19 <212> DNA <213> Human papillomavirus type MM7 <220> <221> -35_signal (222) (1) .. (19) <223> 237-225 <400> 603 tgaacattta ttggatgag 19 <210> 604 <211> 26 <212> DNA <213> Human papillomavirus type MM7 <220> <221> -35_signal (222) (1) .. (26) <223> 267-292 <400> 604 cgtgttacca cctccttcca ccagcc 26 <210> 605 <211> 22 <212> DNA <213> Human papillomavirus type MM7 <220> <221> -35_signal (222) (1) .. (22) <223> 303-324 <400> 605 ctatcgctat ctgcagtccc gt 22 <210> 606 <211> 22 <212> DNA <213> Human papillomavirus type MM7 <220> <221> -35_signal (222) (1) .. (22) <223> 342-363 <400> 606 gggtccttcc gcccctgccc ct 22 <210> 607 <211> 16 <212> DNA <213> Human papillomavirus type MM7 <220> <221> -35_signal (222) (1) .. (16) <223> 375-390 <400> 607 ttatgatggc cttgta 16 <210> 608 <211> 15 <212> DNA <213> Human papillomavirus type MM8 <220> <221> -35_signal (222) (1) .. (15) <223> 93-107 <400> 608 tgctaccaac accga 15 <210> 609 <211> 20 <212> DNA <213> Human papillomavirus type MM8 <220> <221> -35_signal (222) (1) .. (20) <223> 95-114 <400> 609 ctaccaacac cgaatcagaa 20 <210> 610 <211> 22 <212> DNA <213> Human papillomavirus type MM8 <220> <221> -35_signal (222) (1) .. (20) <223> 98-119 <400> 610 ccaacaccga atcagaatat aa 22 <210> 611 <211> 20 <212> DNA <213> Human papillomavirus type MM8 <220> <221> -35_signal (222) (1) .. (20) <223> 110-129 <400> 611 cagaatataa acctaccaat 20 <210> 612 <211> 23 <212> DNA <213> Human papillomavirus type MM8 <220> <221> -35_signal (222) (1) .. (23) <223> 189-211 <400> 612 taaggtccgt ctgactccag agg 23 <210> 613 <211> 19 <212> DNA <213> Human papillomavirus type MM8 <220> <221> -35_signal (222) (1) .. (19) <223> 237-255 <400> 613 tgactcctta ttagatgag 19 <210> 614 <211> 26 <212> DNA <213> Human papillomavirus type MM8 <220> <221> -35_signal (222) (1) .. (26) <223> 267-292 <400> 614 tgttgtgccc cctccctcca caagtt 26 <210> 615 <211> 22 <212> DNA <213> Human papillomavirus type MM8 <220> <221> -35_signal (222) (1) .. (22) <223> 303-324 <400> 615 ctataggtac ttgcagtctc gc 22 <210> 616 <211> 22 <212> DNA <213> Human papillomavirus type MM8 <220> <221> -35_signal (222) (1) .. (22) <223> 342-363 <400> 616 gggggccgcc gccgccaagc ct 22 <210> 617 <211> 16 <212> DNA <213> Human papillomavirus type MM8 <220> <221> -35_signal (222) (1) .. (16) <223> 375-390 <400> 617 ttatgctggc atgtcc 16

Claims (11)

A detector for simultaneously identifying and discriminating human papilloma pathogen (HPV) subtypes contained in a sample, A carrier capable of loading the sample thereon and having a first portion and a second portion; A first type of oligonucleotide loaded into said first portion of said carrier; And And a second type of oligonucleotide loaded on said second portion of said carrier, The oligonucleotides of the first and second species are hybridized with deoxyribonucleic acid (DNA) of the HPV subtypes of the first and second species, respectively, to simultaneously identify and discriminate HPV subtypes contained in the sample. and, The first type of HPV subtype and the second type of HPV subtype are each selected from the following HPV, wherein the base sequence of the first type of oligonucleotide corresponds to the first type of HPV subtype And a nucleotide sequence of the second type of oligonucleotide is selected from the other group consisting of a nucleotide sequence corresponding to the HPV subtype of the second type and its complementary sequence. Featured detector: The detector of claim 1, wherein the carrier is made of nylon or glass. delete The detector of claim 1, wherein the detector is an oligonucleotide test piece. In the method of identifying and discriminating the presence of human papilloma pathogens (HPV) in the sample, Oligonucleotides of the first and second species may be hybridized with deoxyribonucleic acid (DNA) of the HPV subtypes of the first and second species, respectively. Providing a second detection reagent comprising a detection reagent of 1 and the oligonucleotide of the second type; Hybridizing the DNA in the sample and the oligonucleotide; And Removing DNA that cannot hybridize with these oligonucleotides, thereby simultaneously identifying and discriminating these HPV subtypes contained in the sample, The first type of HPV subtype and the second type of HPV subtype are each selected from the following HPV, wherein the base sequence of the first type of oligonucleotide corresponds to the first type of HPV subtype And a nucleotide sequence of the second type of oligonucleotide is selected from the other group consisting of a nucleotide sequence corresponding to the HPV subtype of the second type and its complementary sequence. Featured detector: The method of claim 5, The deoxyribonucleic acid in the sample is a product of a polymerase chain reaction. The method of claim 5, The deoxyribonucleic acid (DNA) in the sample is characterized in that it contains a signal material. The method of claim 5, The signal material is biotin, The method of identifying and discriminating further comprises the step of reacting the biotin with a protein-alkaline phosphatase. The method of claim 7, wherein The signal material is a fluorescent material, The fluorescent material is cyanine 5 (Cyanine 5) characterized in that the method. In a method for detecting a specific human papilloma pathogen (HPV) subtype in a sample, Providing an oligonucleotide in which the nucleotide sequence is specifically hybridized with a deoxyribonucleic acid (DNA) nucleotide sequence unique to a particular HPV subtype; Hybridizing the DNA in the sample with the oligonucleotide; Removing DNA that cannot hybridize with the oligonucleotide in the test reagent; Detecting the remaining DNA to indicate whether the sample contains an HPV subtype, The gene number of the specific HPV subtype and its unique one-time deoxyribonucleic acid are continuous to the position of the gene and are selected from the following 36 HPV subtypes: HPV Gene Number / Gene Length Unique Sequence Location / Sequence Length (Accession number / bp) (loci / bp) 11 type NC 001525/7931 6727-7135 / 4091044-1509 / 46616 type NC 001526/7904 6602-7013 / 4121089-1538 / 45018 type NC 001357/7857 6578-6992 / 4151135-1609 / 47526 type NC 001583/7855 6553- 6967 / 4151093-1522 / 43031 NC 001527/7912 6520-6931 / 4121083-1476 / 39432 NC 001586/7961 6837-7245 / 4091032-1536 / 50533 NC 001528/7909 6559-6967 / 4091100-1532 / 43335 NC 001529/7851 6542-6953 / 4121089-1497 / 40937 NC 001687/7421 6711-7125 / 41539 NC 001535/7833 6605-7019 / 4151149-1593 / 44544 NC 001689/7833 6647-7061 / 4151038-1491 / 45445 NC 001590/7858 6582-6996 / 1451136-1567 / 43251 NC 001533/7808 6486-6897 / 4121092-1506 / 41552 NC 001592/7942 6623-7031 / 4091085-1526 / 44253 NC 001593/7856 6614- 7022/40954 NC 001676/7759 6561-6972 / 4121013-1484 / 47256 NC 001594/7844 6559-6967 / 40958 NC 001443/7824 6608-7016 / 4091104-1536 / 433 59 type NC 001635/7896 6571-6985 / 4151093/1528/43661 type NC 001694/7989 6732-7146 / 4151005-1515 / 51162 type U12499 / 449 21-429 / 40966 type NC 001695/7824 6609-7017 / 4091023-1545 / 52367 D21208 / 7801 6584-6992 / 4091096-1504 / 40968 M73258 / 6042 2582-2996 / 4154990-5350 / 36169 NC 002171/7700 6509-6923 / 4151100-1518 / 4186 NC 000904/8012 6743-7151 Type NC 001711/7905 6549-6963 / 4151149-1608 / 46072 Type X94164 / 7988 6758-7172 / 41574 Type U40822 / 3891 1613-2027 / 41582 Type AB027021 / 7871 6536-6950 / 4151094-1532 / 439CP8061 Type U12479 / 452 21-432 / 412CP8304 Type U12480 / 452 21-432 / 412 21-432 / 412 A composition for detecting a human papilloma pathogen (HPV) subtype contained in a sample, A base sequence for detecting the presence of the HPV subtype comprises an oligonucleotide capable of hybridizing with a deoxyribonucleic acid (DNA) sequence unique to the HPV subtype, The HPV subtype is HPV 26, HPV 32, HPV 37, HPV 53, HPV 61, HPV 62, HPV 66, HPV 67, HPV 69, HPV 70, HPV 72, HPV 74, HPV 82, HPVCP 8061, HPVCP 8304, HPVL1AE 5, HPVMM 4, HPVMM 7, and HPVMM 8, wherein the composition is selected from the group consisting of: