KR100385832B1 - Electrochemical Biosensor Test Strip with Recognition Electrode and Readout Meter using This Test Strip - Google Patents

Abstract

The present invention relates to an electrochemical biosensor system capable of selectively quantitatively analyzing blood glucose, cholesterol, and the like in blood. In the electrochemical biosensor test strip according to the present invention, a recognition electrode indicating a fixed reagent for quantitative analysis is formed at a predetermined position, and the tester inserts a test by automatically recognizing the position of the recognition electrode. The use of the strip is determined, and a predetermined algorithm is performed. The biosensor system according to the present invention has the advantage of being able to quantitatively analyze various blood components such as blood sugar, cholesterol, GOT, GPT, etc. using a single measuring device.

Description

Electrochemical Biosensor Test Strip with Recognition Electrode and Readout Meter using This Test Strip}

The present invention relates to an electrochemical biosensor test strip capable of selectively quantitatively analyzing specific substances in a biological sample, such as blood glucose, cholesterol, and the like, and an electrochemical biosensor measuring device using the test strip.

Recently, in the medical field, many electrochemical biosensors are used to analyze biological samples including blood. Among them, electrochemical biosensors using enzymes are the most widely used in hospitals and clinical laboratories because of their simplicity of application, excellent measurement sensitivity, and quick results. Enzyme analysis applied to electrochemical biosensors can be classified into colorimetric method and electrochemical electrode method. The colorimetric method generally takes longer time than the electrode method, and it is difficult to analyze important biomaterials due to measurement errors due to turbidity of biological samples. Therefore, in recent years, the electrode method of forming an electrode system using a screen printing method, and then fixing an analytical reagent on the electrode, applying a predetermined potential after the sample is introduced, and quantitatively measuring a specific substance in the sample is an electrochemical biotechnology. It is widely applied to sensors.

US Patent No. 5,437,999 "Electrochemical Sensor" describes an electrochemical biosensor test strip having a precisely defined electrode region by newly applying a technique commonly used in the PCB industry to be suitable for an electrochemical biosensor test strip. It is. This electrochemical biosensor test strip can perform highly accurate electrochemical measurements with very low sample volumes.

FIG. 1 illustrates an exploded perspective view of an opposing electrode type in the electrochemical biosensor test strip of the US patent, and FIG. 2 illustrates a state in which the test strip of FIG. 1 is coupled. Here, an electrode in which a reaction occurs in a test strip composed of a two-electrode system is called a working electrode, and another electrode is called a reference electrode or an auxiliary electrode.

1 and 2, the structure of the insulator substrate on which the reference electrode is formed, that is, the reference electrode element 10 is an insulator on which a working electrode is formed by the spacer 16. It is spatially separated from the substrate, that is, the working electrode element 20. The spacer 16 is usually attached to the reference electrode element 10 during the manufacturing process, but is shown separately from the reference electrode element 10 in FIG. The first cutout 13 in the spacer 16 forms a capillary space 17 when positioned between the reference electrode element 10 and the working electrode element 20. The first cutting portion 22 in the working electrode element 20 exposes the working electrode region, which is exposed in the capillary space 17. The first cutout 13 in the spacer 16 defines the reference electrode region 14, shown in dashed lines in FIG. 1, when attached to the reference electrode element 10, which also defines the reference electrode region 14. It is exposed to the capillary space 17. The second cut portions 12 and 23 expose the reference electrode region 11 and the working electrode region 21, respectively, and connect the electrochemical biosensor test strip 25 to a biosensor measuring instrument (or meter and power source). Act as a contact pad.

In the coupled state of FIG. 2, the electrochemical biosensor test strip 25 has a first opening 27 on one side. In addition, the hole 24 in the working electrode element 20 and the hole 15 in the reference electrode element 10 coincide to form the second opening 26. In use, the sample containing the analyte can be introduced into the capillary space 17 through the opening 26 or 27. In this case, the sample is spontaneously introduced into the electrochemical biosensor test strip by capillary action. As a result, the electrochemical biosensor test strip automatically controls the volume of sample to be measured without user intervention.

These electrochemical biosensor test strips can perform highly accurate electrochemical measurements and, in addition to the advantages of requiring a small amount of sample, separate the manufacturing process of the two electrodes, thereby applying the compounds related to the working electrode and the reference electrode. The advantage is that it can be separated.

However, the conventional electrochemical biosensor measuring system mostly measured only one substance in one measuring instrument, and a high level of attention was required of the user to obtain accurate results by treating various substances in one measuring instrument. As a result, companies that produce biosensors have had to make significant efforts in the manufacture of their instruments to eliminate possible malfunctions when users use them.

European Patent No. 0471986 relates to a patent for a system for measuring blood glucose in a blood by using a disposable test strip, which determines whether blood is introduced into the test strip by measuring a resistance value between a pair of electrodes, and also a test. Disclosed is a strip that can perform various tasks such as changing a measurement mode of a meter or correcting a measured value of a meter by attaching a strip-shaped resistor to the strip.

US Pat. No. 4,999,582 relates to a circuit for determining whether a test strip is inserted into a biosensor measuring device and applying a reaction voltage to both ends of the test strip.

U. S. Patent No. 5,438, 271 relates to a circuit for determining whether a strip is properly inserted into a biosensor meter and for distinguishing whether the inserted strip is a test strip or a strip for calibrating the meter.

Until now, the biosensor measuring device has only a function of discriminating whether a strip is inserted and a function of distinguishing whether the strip inserted into the measuring device is a test strip or a calibration strip for calibrating the measuring device. Could not be analyzed using the biosensor measuring device.

Accordingly, an object of the present invention is to provide an electrochemical biosensor strip capable of automatically recognizing a substance to which a reagent fixed on a test strip is intended to detect.

It is also an object of the present invention to provide a biosensor measuring device capable of selectively quantitatively analyzing a plurality of substances according to a test strip.

In addition, an object of the present invention is to provide a test strip for calibration used to automatically calibrate the meter so that the meter can accurately measure the current flowing in the test strip in the reaction of the material and the reagent to be analyzed at the concentration of the analyte accurately. do.

1 is an exploded perspective view of a conventional electrochemical biosensor test strip.

Figure 2 is a perspective view showing a combined state of the test strip of Figure 1;

Figure 3 shows an embodiment of a test strip according to the present invention, Figure 3a is a plan view, Figure 3b is a left side view.

4 is a plan view showing two test strips having different positions of the recognition electrode.

Figure 5 is a perspective view of the electrical connection means constituting the socket device of the measuring device according to the present invention.

Figure 6 is a plan view of the electrical connection means constituting the socket device of the measuring device according to the present invention.

Figure 7 is a perspective view of the crimping means constituting the socket device of the measuring device according to the present invention.

8 is a circuit diagram of a measuring device according to the present invention.

9A is a waveform diagram of an operating voltage applied to the working electrode, and FIG. 9B is a waveform diagram of current flowing through the working electrode.

10 is a flowchart illustrating the operation of the measuring device according to the present invention.

11 is a plan view of a test strip for correction according to the present invention.

* Description of the symbols for the main parts of the drawings *

33: working electrode 34: reference electrode

35, 40a, 40b: recognition electrode 36: capillary portion

37 capillary space 38 reagent

50: socket device 60: electrical connection means

70: crimping means

In order to achieve the object as described above, the electrochemical biosensor test strip according to the present invention is a test strip on the test strip to indicate a substance (hereinafter referred to as an analyte) to be analyzed by the reagents immobilized on the test strip. A first feature is that the recognition electrode is formed at a predetermined position of the first electrode.

In addition, the biosensor measuring device according to the present invention automatically detects the position of the recognition electrode according to the insertion of the test strip, thereby identifying the analyte in the test strip, and selecting and performing an algorithm corresponding thereto to quantitatively analyze the analyte. It is a 2nd characteristic.

In addition, the socket device of the biosensor measuring device according to the present invention is electrically connected to the electrode on the test strip and the connecting terminal on the electrical connection means because the connection terminal is formed by the electrical connection means formed in the pattern on the PCB and the test strip to the electrical connection means. A third feature is that it is composed of the pressing means to make.

In addition, the electrochemical biosensor measuring device according to the present invention is a measuring device using an electrochemical biosensor test strip according to a first aspect, comprising: means for detecting the recognition electrode and an operating voltage generation circuit for applying an operating voltage to the working electrode; And voltage conversion means for converting a current flowing through the working electrode into an analog voltage signal, an A / D converter for converting an analog voltage signal generated by the voltage conversion means into a digital voltage signal, and the test strip. When inserted into the measuring instrument (t0), the operating voltage generating circuit causes the first voltage to be applied to the working electrode, and when the sample is introduced (t1), after a time according to the position of the detecting electrode detected by the detecting electrode detecting means. at (t2) the operating voltage generating circuit applies the second voltage to the working electrode for a predetermined time, and then at (t3) the operation The voltage generating circuit applies a third voltage to the working electrode, reads the digital signal output from the A / D converter after a predetermined time (t4) from the third voltage application time t3, and It characterized in that it comprises a controller for measuring the concentration of the analyte in accordance with the position.

Hereinafter, the present invention will be described in detail with reference to the embodiments shown in the drawings. However, the examples described below are for explaining the present invention in detail, and the scope of the present invention is not limited by the following examples.

Figure 3 shows an embodiment of a test strip according to the present invention, Figure 3a is a plan view, Figure 3b is a left side view. In FIG. 3A, 33 denotes a working electrode in which a redox reaction between an analyte and a reagent occurs, 34 denotes a reference electrode, and 35 denotes a recognition electrode.

The recognition electrode in FIG. 3A is a predetermined position on the test strip, determined by which reagent 38 is fixed in the capillary section 36 across the working electrode 33 and the reference electrode 34 to detect a substance. Is formed. When the test strip is inserted into the biosensor measuring device, the biosensor measuring device identifies the position of the recognition electrode on the test strip, so that the test strip is able to identify which material to analyze.

As the material of the insulator substrate 31 used in the present invention, any insulator can be used, but in order to simultaneously manufacture a large amount of the electrochemical biosensor test strip of the present invention, it is suitable for roll processing and rigidity as a support. It is preferred to have. Preferably, a polymer compound such as polyester, polycarbonate, polystyrene, polyimide, poly vinyl chloride, polyethylene, or the like It may be used, in particular polyethylene terephthalate (polyethylene telephthalate) can be used.

The electrode strips 33, 34, 35 on the insulator substrate 31 are preferably formed by sputtering using a shadow mask. That is, when the shadow mask having the electrode strip patterned is placed on the insulator substrate 31, the normal electric sputtering is performed, and then the shadow mask is removed, and the electrode strips 33, 34, 35 are disposed on the insulator substrate 31. This is well formed. At this time, it is preferable to use a thin stainless steel plate as a shadow mask. The thickness of the shadow mask is preferably about 0.2 mm. If the electrode strip is formed by sputtering using a shadow mask, a very fine electrode of about 0.1 mm may be easily formed without an additional process.

As the material of the electrode used in the present invention, it is preferable to use a noble metal such as palladium, platinum, gold or silver. This is because rare metals have excellent electrochemical properties such as excellent stability and reproducibility in the surface area of the electrode and properties that are difficult to oxidize. In particular, it is preferable to use gold as a material of an electrode. Gold is relatively inexpensive among rare metals, is easy to process, has good adhesion to plastics, and has high electrical conductivity. Therefore, even if a thin gold electrode is formed to a thickness of approximately 100 nm by sputtering, the electrode has low electrical resistance and is mechanically and firmly adhered to an insulator substrate such as a plastic film, and thus is suitable as a disposable electrode.

Reagents 38 used herein may include enzymes, redox mediators, hydrophilic polymers, and surfactants. The enzyme used varies depending on the substance to be detected or analyzed. For example, if you want to detect or analyze glucose, you can use glucose oxidase. Redox mediators used include potassium ferricyanide, imidazole osmium mediators as disclosed in US Pat. No. 5,437,999, and the like. Reagent 38 may further include buffers, film formers, and surfactants in addition to enzymes and redox mediators. The buffer solution serves to maintain a constant condition such as pH while the reagent 38 and the sample to be analyzed are reacted. A hydrophilic polymer compound is necessary to easily fix the reagent on the electrode. This is to make it easier when the sample to be analyzed in the capillary space is introduced by capillary action. For example, reagents for detecting or analyzing glucose are formed by mixing potassium ferricyanate, potassium phosphate buffer, cellulose, hydroxyethyl cellulose, triton X-100 surfactant, sodium succinate and glucose oxidase See US Pat. No. 5,762,770 for the specific preparation thereof and examples of enzymes and redox mediators that may be used.

On the other hand, the principle of measuring the concentration of the analyte to be detected and analyzed in the sample using the electrochemical biosensor test strip of the present invention is as follows. To detect and analyze glucose in blood samples, use glucose oxidase as enzyme and potassium ferricyanide as redox mediator. For example, glucose is oxidized and ferricyanide is reduced to ferrocyanide. At this time, glucose oxidase acts as a catalyst for this reaction. After a certain reaction time, when a voltage is applied to the two electrodes by a power source, current is generated by an electron transition due to the reoxidation of ferrocyanide. At this time, the voltage applied to the two electrodes by the power source is appropriately 300 ㎷ or less, of which 100 ㎷ is used in consideration of the characteristics of the medium.

The current measured in this manner can be related to the concentration of analyte in the sample by applying an algorithm stored in the biosensor meter. Alternatively, by integrating the current with respect to the measurement time of a certain time in the relationship curve of the measured current with respect to the measurement time, the total amount of charge during this time (the amount of charge is directly proportional to the concentration of the analyte) can be obtained. .

Figure 4 shows an example of two electrochemical biosensor test strips having different positions of the recognition electrode according to the present invention, Figure 4a is a test strip for blood glucose measurement, Figure 4b is a test strip for cholesterol measurement, Figure 4c is a calibration test strip (hereinafter referred to as a "check strip") for providing a meter with information about the test strip to calibrate the meter so that the meter can change the current flowing in the test strip to the correct concentration. The reagents fixed to the capillary part differ according to the analyte, and the recognition electrodes 40a, 40b, and 40c are formed at different positions as shown in FIG. will be.

The position of the recognition electrode may be two-dimensionally arranged on the test strip. If the recognition electrodes are two-dimensionally arranged, it is easy to make the recognition electrodes having various positional information on the limited test strip.

5 is a perspective view showing a socket device of a conventional measuring device. As shown in FIG. 5, since the socket device 50 of the conventional measuring device has a metal strip 52 arranged one-dimensionally in the width direction of the test strip, the electrode on the test strip that the socket device can tolerate. Was limited in number. In addition, the production was difficult and cost a lot of manufacturing costs.

In the present invention, the biosensor measuring device socket device is composed of an electrical connection means of the connection terminal is made of a PCB pattern, and crimping means for electrically connecting the electrode of the test strip to the connection terminal of the electrical connection means by pressing the test strip to the electrical connection means. do.

6 is a plan view showing a PCB pattern of the electrical connection means constituting the measuring instrument socket apparatus according to the present invention. In Fig. 6, the connecting terminal is formed in a pattern on the PCB so as to correspond to the positions of the working electrodes and reference electrodes on the test strip and the recognition electrodes when the test strip is completely inserted into the socket device of the measuring instrument.

4 and 6, the electrical connection between the test strip and the biosensor measuring device will be described in detail. When the test strip of FIG. 4 is inserted into the socket device of the biosensor measuring device including the electrical connecting means of FIG. The working electrode 33 is electrically connected to the working electrode connecting terminal 62 on the electrical connecting means 50. The reference electrode 34 is the reference electrode connection terminal 64, the blood sugar display recognition electrode 40a is the first recognition electrode connection terminal 66, and the cholesterol display recognition electrode 40b is the second recognition electrode It is electrically connected to the connection terminal 68. In FIG. 6, the connection terminal for the recognition electrode may be easily formed on the PCB in two dimensions. Therefore, a large number of connection terminals for the recognition electrodes can be made in the electrical connection means of the socket device, so that it is possible to quantitatively analyze various materials using one measuring device. In addition, since the connection terminals can be made two-dimensionally, the machining margin is large.

7 is a perspective view showing a pressing means for pressing the test strip in order to electrically connect the test strip of FIG. 4 to the electrical connecting means of FIG. 6 according to the present invention. The electrical connection means of FIG. 6 is installed below the crimping means shown in FIG. 7. When the test strip is inserted in the A direction, the crimp section 71 is lifted up, whereby the connecting section 72 is subjected to a torsional force, and by this force, the crimp section 71 tests toward the electrical connection means installed below. The strip is pushed. In FIG. 7, 73 is a fixing part, and is inserted into a groove made in the electrical connecting means 60, thereby serving to fix the crimping means 70 to the electrical connecting means 60.

8 is a circuit diagram of an electrochemical biosensor measuring device according to the present invention. When the test strip 30 as shown in FIG. 4A is properly inserted into the electrical connection part of the measuring device 80, the voltage of the contact point A connected to the recognition electrode 35 on the test strip 30 is from 5V to 0V. Falls. This change in voltage is detected at I / O 85 and known to microprocessor 86, so it is recognized that the test strip has been inserted into the electrical connection of the meter. In addition, since the I / O 85 maintains 0V and the I / O 84 maintains 5V, the microprocessor 86 may recognize that the inserted test strip is a test strip for measuring blood glucose. When the test strip of FIG. 4B is inserted, the microprocessor automatically recognizes the test strip for measuring cholesterol because the I / O 25 of the measuring instrument maintains 5V and the I / O 24 maintains 0V. When the test strip of FIG. 4C is inserted, the microprocessor automatically recognizes that the inserted test strip is a check strip because both the electrical connection I / O 24 and the I / O 25 of the measuring instrument maintain 0V. The check strip is used to calibrate the meter to provide the meter with information about the test strip so that the meter can accurately measure the concentration of the analyte.

As shown in FIG. 4A, when a test strip for measuring blood glucose is inserted into an electrical connection of the measuring device 80, the switch of the operating voltage generator 81 is closed to the operational amplifier OP, and the operating voltage generator 81 is closed. Generates 300 ms. The generated voltage applies an operating voltage of 300 kV between the reference electrode 33 and the working electrode 34 of the test strip 30 by the feedback characteristic of the operational amplifier OP.

From this time, the blood is waited to be injected into the reaction section 36 of the test strip. When blood is introduced at t1 in FIG. 9B, charges are generated by a chemical reaction between the reagent fixed in the reaction unit and the blood, and the charges form a current by the operating voltage applied to the working electrode. This current is input to the A / D converter 83 via the resistor Rf. The microprocessor 86 reads the change in the value of the A / D converter 83 to measure the amount of current, and determines that blood has been injected when the current reaches a predetermined value or more (t2). If no blood is injected, there is no theoretical amount of current flowing, but some current may flow through the input terminal of the A / D 83 converter due to noise. Therefore, in order to prevent malfunction due to noise, as shown in FIG. 9B, it is determined that blood is input only when a predetermined current value or more is read by the A / D converter 83. At this time, since the current range varies depending on the substance to be analyzed, the threshold current value (ith) used to determine that the blood is injected varies depending on the type of substance to be analyzed. Since the biosensor measuring device according to the present invention can automatically distinguish which material the test strip is for analyzing by the recognition electrode 35, the threshold current value is read by reading a value previously stored in the measuring device. Can be applied differently depending on the analyte.

At the time point t2 recognized by the microprocessor 86 as the blood is injected, the voltage of the operating voltage generator 81 changes to almost 0V. At this time, electric charges are accumulated around the working electrode by the chemical reaction between the reagent in the reaction unit 36 and the blood. After the reaction for a predetermined time, the voltage of the operating voltage generator 81 is changed to 300 kW at time t3. As shown in FIG. 9A, when 300 kV is applied as an operating voltage, current flows to the working electrode as shown in FIG. 9B, and a current flowing through the test strip 10 is measured after a predetermined time (t4). The microprocessor 86 has a ROM (not shown) in which the relationship of the current according to the concentration of the analyte is stored. Therefore, the concentration of blood glucose in the blood can be measured by reading the current at time t4. If a test strip for cholesterol as shown in Figure 4b was used, the current at time t4 is related to the cholesterol concentration in the blood.

10 is a flowchart showing a driving sequence of the measuring device according to the present invention. When the power button is pressed, the meter checks whether the test strip is inserted 110 by blinking an icon to insert the test strip on the LCD. When the test strip is inserted, the buzzer sounds and checks whether or not a recognition electrode is formed at the blood glucose display position and the cholesterol display position on the test strip (112, 114, 116, 118). If the inserted test strip has a recognition electrode formed at both the blood glucose display position and the cholesterol display position, the check strip processing routine is executed (120). If the recognition electrode is formed only at the cholesterol display position, the cholesterol processing routine is executed. (122) If the recognition electrode is formed only at the blood glucose display position, blood sugar processing routine is executed (124). After the execution of the processing routine, the test strip is removed and the instrument is automatically switched off after a certain period of time with no new test strip inserted. When the new test strip is inserted, the buzzer is sounded again and steps 112, 114, 116, and 118 of recognizing the position of the recognition electrode on the test strip are performed again.

In operation 110, the method may further include checking whether the memory button of the meter is pressed instead of checking whether only the test strip is inserted. When the memory button is pressed, the value stored in EEPROM is read.

If the test strip is not inserted for a certain time, the alarm sounds and the power is automatically turned off. If the test strip is removed from the meter during the measurement, the process starts from step 110 again.

The execution of the blood glucose processing routine 124 is as follows. First, the LCD displays blood sugar, and checks whether the sample is inserted by blinking an icon to insert a sample such as blood into the test strip. When the sample is added, wait for a period of time, for example 15 seconds, for the sample to react with the reagent. After 15 seconds have elapsed, a voltage is applied between the working electrode and the reference electrode on the test strip for a predetermined time, for example, 15 seconds, and the current flowing through the electrode on the test strip is measured and converted into a blood glucose value. Save to EEPROM and display on LCD.

The execution of the cholesterol processing routine 122 is the same as that of the blood glucose processing routine 124 except that the correspondence between the current flowing through the electrode on the test strip and the cholesterol value is different.

11 is a view showing an example of a check strip according to the present invention. In FIG. 11, 111 is a recognition electrode, 112 is a reference electrode, 113 is a working electrode, and 114 is a resistance for giving information about a test strip to a measuring instrument. As described above, when the check strip 110 is inserted into the measuring device as shown in FIG. 8, both A and B points are set to 0 V, and it is recognized that the test strip inserted by the microprocessor 86 is a check strip. In the check strip 110, the reference electrode 112 and the working electrode 113 are connected to the resistor 114. The resistor 114 allows a constant current to flow through the working electrode 113 when the working voltage is applied to the working electrode 113. This current is converted into a voltage by the operational amplifier OP, converted into a digital voltage signal by the A / D converter 83, and read by the microprocessor 86, so that the information on the test strip is recognized by the measuring instrument. . Using this information, the meter can calculate the concentration of the analyte more accurately.

According to the present invention as described above, the use of the test strip, that is, the analyte can be automatically recognized without a button operation on the biosensor measuring device, blood glucose, cholesterol, as well as various blood components such as GOT, GPT using a single measuring device Can be easily quantitatively analyzed. In addition, since the meter does not need a separate socket, the manufacturing cost is very low. In addition, check strips can be easily created using the recognition electrodes and resistors, from which the exact concentration of the analyte can be calculated.

Claims (15)

In an electrochemical biosensor test strip that is used with an electrochemical biosensor measuring device to measure the concentration of analyte by an electrochemical method, A first insulator substrate, A plurality of electrodes formed side by side in the longitudinal direction on the first insulator substrate, A reagent fixed to the plurality of electrodes on the first insulator substrate, the reagent reacting with the analyte to allow a current corresponding to the concentration of the analyte to flow across the electrode; Since a portion of the first insulator substrate inserted into the electrochemical biosensor measuring device is formed at a position determined according to the analyte, a recognition electrode for displaying the type of the analyte to the electrochemical biosensor measuring device is provided. An electrochemical biosensor test strip comprising: The method of claim 1, An electrochemical biosensor test strip further comprising a second insulator substrate on the first insulator substrate, wherein the first insulator substrate and the second insulator substrate form a sample inlet at a position of the first insulator on which the reagent is fixed. . The method of claim 2, The sample inlet is formed of a capillary electrochemical biosensor test strip. The method of claim 1, The recognition electrode is an electrochemical biosensor test strip arranged on a predetermined position two-dimensionally on the first insulator. In the electrochemical biosensor measuring device used in conjunction with the electrochemical biosensor test strip having a recognition electrode and a working electrode and a reference electrode indicating the type of the analyte because it is formed at a predetermined position according to the analyte, A socket device electrically connected to an electrode on the test strip according to the insertion of the electrochemical biosensor test strip and generating an analyte reading signal by detecting a position of the recognition electrode; Current measuring means for measuring a current flowing in the working electrode electrically connected through the socket device; Analysis means for analyzing the concentration of the analyte based on the current value measured by the current measuring means and the analyte reading signal generated by the socket device; Display means for displaying a signal generated by said analysis means; Biosensor measuring instrument comprising. The method of claim 5, The socket device is a crimping means for electrically connecting the connecting terminal on the electrical connecting means and the electrode on the test strip, so that the electrical connection means formed in the PCB pattern and the test strip is crimped to the electrical connection means. Biosensor measuring device comprising a. In the electrochemical biosensor system, Equipped with an electrochemical biosensor test strip and a biosensor meter, The electrochemical biosensor test strip A first insulator substrate, A plurality of electrodes formed side by side in the longitudinal direction on the first insulator substrate, A reagent fixed to the plurality of electrodes on the first insulator substrate, the reagent reacting with the analyte to allow a current corresponding to the concentration of the analyte to flow across the electrode; Since it is formed at a predetermined position according to the analyte in the portion of the first insulator substrate inserted into the electrochemical biosensor measuring device, and comprises a recognition electrode for displaying the type of the analyte to the electrochemical biosensor measuring device, The biosensor measuring device In the biosensor measuring device, A socket device electrically connected to an electrode on the test strip according to the insertion of an electrochemical biosensor test strip, and configured to detect test object information on the test strip and generate a test strip insertion signal and a read object signal; Current measuring means for measuring a current flowing in an electrode on the test strip electrically connected through the socket device; Analysis means for analyzing the concentration of the target material based on the current value measured by the current measuring means and the target material reading signal generated by the socket device; Display means for displaying a signal generated by said analysis means; Electrochemical biosensor system, characterized in that. In the socket device of the electrochemical biosensor measuring device, Electrical connection means having a connection terminal formed of a PCB pattern, Since the test strip is pressed to the electrical connecting means, the pressing means for electrically connecting the connection terminal on the electrical transmission means and the electrode on the test strip to each other Included socket device. In the method of driving an electrochemical biosensor measuring device, Checking whether a test strip has been inserted into the meter when the power button is pressed; Checking the position of the recognition electrode on the test strip when the test strip is inserted; Executing the processing routine according to the position of the recognition electrode identified in the step; Method of driving a biosensor measuring device comprising. The method of claim 9, The step of checking the test strip further comprises the step of turning off the meter if the test strip is not inserted for a predetermined time elapses. The checking of the test strip may include checking whether the memory button of the meter is pressed; And when the memory button is pressed, reading and displaying a value stored in a storage location in the measuring device. The method of claim 9, And if the test strip is removed from the meter during the measurement, re-running from the test strip checking step. The method of claim 9, The treatment routine execution step is to check whether the sample is added; Analyzing the concentration of the target material by measuring a current by applying a predetermined voltage to an electrode on the test strip after waiting for a predetermined time for reacting the sample and the reagent when the sample is added; The process of outputting the concentration value of the target material Method of driving a biosensor measuring device comprising. In the measuring device using the electrochemical biosensor test strip according to claim 1, Means for detecting a position of the recognition electrode to recognize an analyte in the test strip; An operating voltage generating circuit for applying an operating voltage to the working electrode; Voltage conversion means for converting a current flowing through the working electrode into an analog voltage signal; An A / D converter for converting an analog voltage signal generated by the voltage converting means into a digital voltage signal; When the test strip is inserted into the measuring instrument (t0), the operating voltage generating circuit causes a predetermined operating voltage to be applied to the working electrode, and a sample is introduced (t1) to output the digital from the A / D converter. When the voltage signal reaches a predetermined threshold voltage according to the analyte (t2), the operation voltage generation circuit is not allowed to apply any voltage to the operation electrode for a predetermined time, after which the operation voltage is generated. Causing the circuit to apply the operating voltage to the working electrode again, and reading the digital voltage signal output from the A / D converter after a predetermined time (t4) from the time t3 of applying the operating voltage to the analyte. Electrochemical biosensor measuring device comprising a controller for measuring the concentration of. In the electrochemical biosensor test strip, The test strip is formed at a predetermined position on the first insulator substrate, the two electrodes formed in parallel in the longitudinal direction on the first insulator substrate, the resistance connected between the two electrodes, and the first insulator substrate. An electrochemical biosensor test strip comprising a recognition electrode indicating that the test strip is for calibrating the meter.