KR100375755B1 - The method for the development of carriers for the wastewater treatment and the immobilized carriers containing microorganisms - Google Patents

Abstract

The present invention relates to a method for preparing a microorganism immobilization carrier for the purification of wastewater and a microorganism immobilization carrier prepared therefrom.

The composition is

Saponified compounds or commercially purchased soap raw materials

1-50% by weight of microorganisms having emulsifying power and component degradability, 0.5-30% by weight of emulsifier, and 2-20% by weight of mineral salt, are mixed and solidified to 100% by weight to prepare a microorganism immobilization carrier for wastewater purification. Is provided.

Furthermore, there is provided a microorganism immobilization carrier prepared by the above method.

The microorganism immobilization carrier prepared as described above is emulsified by loosening of the soap component while emulsifying the component to be treated in the wastewater, and at the same time, the component decomposition microorganism is released together to re-emulsify and decompose the treated component and easily decomposes in nature. It is made of materials that can be environmentally friendly.

Description

The method for the development of carriers for the wastewater treatment and the immobilized carriers containing microorganisms}

The present invention relates to a method for preparing a microorganism immobilization carrier for waste water purification and to a microorganism immobilization carrier prepared therefrom, and more particularly, to manufacturing a microorganism immobilization carrier having both emulsifying power and component degrading power. It is about removing it.

Currently, restaurants, especially most stores that use ramen and oil, discharge oil-containing wastewater into sewers without additional treatment, so that oil and oil solids are hardly decomposed in nature, causing serious environmental pollution. there is a problem.

In this case, even if only waste oil is collected and the wastewater is treated, the process of separating it from water is not easy and expensive equipment is required to be installed separately. Also, in Japan, a collection tank and a temporary storage tank are installed prior to sewage treatment. In this case, since the oil forms a separate layer from water and agglomerates to a wall or a pipe of most upper layers, there is little water treatment effect and it is known that an excessive amount of emulsion type polymer flocculant should be administered.

Even with such chemicals, there is a problem that causes secondary contamination, rather than complete treatment. Therefore, there is a need for a method for effectively removing such hardly decomposable substances, and there is a need to effectively treat toxic substances contained in large amounts of wastewater.

Accordingly, an object of the present invention is to provide a method for preparing a microbial immobilization carrier for wastewater purification that can effectively treat toxic and hardly degradable components to be removed in wastewater.

Another object of the present invention is to provide an economical microorganism immobilization carrier which can be used for a long time by simple one-time administration.

Another object of the present invention is to provide a microorganism immobilization carrier for environmentally friendly wastewater purification since it is easily degradable in nature.

According to one aspect of the invention,

To a saponified compound prepared by heating or stirring at least one selected from a reaction mixture of caustic soda and oil, starch, peels and diatomaceous earth or commercially available soap raw materials.

1-50% by weight of microorganisms having both emulsification and component decomposition

0.5-30% by weight emulsifier, and

Prepared by mixing and solidifying mineral salt 2-20% by weight, to a total of 100% by weight,

Here, the microorganisms having emulsifying power and component degrading power are those of the genus Bacillus in order to treat oil components in wastewater.Bacillussp.) B4, Bacillus 9 (Bacillussp.) C9 and Candida.Candidasp.) Using a blend of SY-16,

When treating aromatic hydrocarbons such as phenol, benzene and naphthalene, Pseudomonas sp., Acinetobacter sp., Candida sp., Candine sp., Corynebacterium sp., And Using a blend of Mycobacterium sp.

For the papermaking process, a combination of Bacillus sp., Pseudomonas sp. And Flavobacterium sp. Is used.

To process crude oil, Bacillus sp., Nocardia sp., Corynebacterium sp., Candida sp. And Pseudomonas sp. Formulated formulations,

For the purpose of removing odor, a combination of Thiobacillus sp., Sulfolobus sp., Rodococcus sp. And Brevibacterium sp. ,

In order to remove the nitric acid stream used in the nitro when seutiseu (Nitrocystis sp.) And and to remove the sulfur compounds using a thio Bacillus (Thiobacillus sp.), And

The emulsifier is selected from nonionics such as Tween 20 and Tween 80, cationic emulsifiers such as amine oxides and anionic emulsifiers such as alkyl ester sulfonates, alkyl ester persates, and

The mineral salt is provided with a method for producing a microorganism immobilization carrier for waste water purification, characterized in that one selected from calcium, iron, magnesium, aluminum, phosphate.

According to the second aspect of the present invention, there is provided a microorganism immobilization carrier produced by the above method.

EMBODIMENT OF THE INVENTION Hereinafter, this invention is demonstrated.

The present invention provides a microorganism immobilization carrier which is easily degradable in nature and effective for wastewater treatment by adding microorganisms, emulsifiers and mineral salts capable of treating toxic or hardly degradable components in wastewater to the carrier prepared by saponification.

In order to provide such a microorganism immobilization carrier, a support is first prepared by a saponification reaction. Here, the support may use one or more selected from a reaction mixture of caustic soda and oil, starch, peels and diatomaceous earth, and may use a commercially available soap. As used herein, the term peeled skin means a grain foil such as skim steel, but is not limited thereto. Such peelings may be used just as they are, but since the particles are rough, it is preferable to grind them to about 100-200 mesh for uniformity during the reaction.

In the case of selecting starch, it is dissolved in 25% or more of water and used in the same manner as in the conventional method.

On the other hand, in the case of using a reaction mixture of caustic soda and oil, it is preferable to add more caustic soda to saponification reaction and to solidify quickly, but considering the economic aspect, the mixing ratio of 1: 1-1: 4 It is preferable that it is a weight ratio.

After dissolving the prepared support, it is added to a total of 100% by weight of microorganisms, emulsifiers and mineral salts having emulsifying power and component degrading power. Here, to melt the support, a generally known method, for example, a method of heating for 10-60 minutes at a temperature of 10-120 ° C. or stirring for 20 minutes to 2 hours without heating may be used.

The microorganisms having emulsifying and constitutive degradability include, for example, Bacillus as an oil degradable component for treating oil. B4 and Bacillus. C9 and Candida as an emulsifying component. It is recommended to use SY-16.

In addition, in order to treat aromatic hydrocarbons such as phenol, benzene and naphthalene, microbial blends of Pseudomonas genus, Acinetobacter genus, Candida genus, Corynebacterium genus and Mycobacterium genus can be used.

Microbial blends of the genus Bacillus, Pseudomonas and Flavobacterium can be used to treat papermaking in wastewater.

In addition, microbial blends of the genus Bacillus, Nocardia, Corynebacterium, Candida and Pseudomonas can be used to process crude oil components.

For the purpose of removing odors, microbial blends of the genus Thiobacillus, sulfobusus, Rhodococcus and Brevibacterium can be used.

In addition, nitrosistis can be used to remove nitrates, and thiobacilli can be used to remove sulfur compounds.

Such microorganisms (the combinations thereof) are preferably coated and used for the purpose of enduring harsh environments and protecting them from harmful environments. Such coatings are polysaccharides as coating materials in Korean Patent Application No. 2000-17801, which the inventors have filed. Examples using the same are disclosed.

It is preferable to use these microorganisms in an amount of 1-50% by weight based on the total weight of the microbial blend, but less than 1% by weight is insufficient in emulsifying and degrading effects. Therefore, it is not preferable. More preferably 5-27% by weight based on the total weight of the microbial blend.

The emulsifier is generally available and can be selected from known nonionic, cationic and anionic emulsifiers, for example Tween 20 and Tween 80 for nonionics, amine oxides for cationics and anionic As the emulsifier, alkyl ester sulfonates and alkyl ester persates can be used.

In addition, the amount is preferably 0.5-30% by weight based on the total weight of the microbial blend, less than 0.5% by weight can not exhibit the emulsification effect, more than 30% by weight does not have a significant improvement in the emulsification power compared to the amount used Not desirable More preferably, 5-35% by weight is used.

Furthermore, mineral salts selected from calcium, iron, magnesium, aluminum, and phosphate can be used to improve strain stability, the amount of which is preferably 2-20% by weight based on the total weight of the microbial blend, but less than 2% by weight. In, there is no effect on strain stability, and if it exceeds 20% by weight, the microorganism may be inhibited. More preferably, 5-15% by weight is used.

Among them, the most ideal addition ratio is 70% by weight soap support, 10% by weight microorganism, 15% by weight emulsifier and 5% by weight mineral salt.

Thus, the carrier is prepared by blending microorganisms, emulsifiers and mineral salts in the soap and then fixing the blend. In this case, when a mixture of caustic soda and oil is not used, that is, when a starch, diatomaceous earth, peeling, etc. is prepared to prepare a microorganism immobilization carrier, it is naturally melted when left after blending to produce an immobilization carrier.

The microbial stationary phase carrier prepared in this way can be administered directly to the waste water, and the wastewater is disposed in a sewage treatment tank such as an aeration tank as well as a collection tank or a temporary storage tank. Emulsification, component decomposition microorganisms are emulsified again while emulsifying and discharged with the treated water as a result is characterized in that it can be used conveniently.

For example, it treats oils in wastewater and contains Bacillus B4 and Bacillus C9 and Candida as microbial blends. When SY-16 is used, the soap component and emulsifier are released to emulsify the oil, while Candida SY-16 reemulsifies the oil, and Bacillus decomposes the oil emulsion.

In addition, if 100g of the microorganism immobilization carrier prepared according to the present invention is applied, the oil in 1 ton of wastewater can be completely decomposed in about 7 days, and when applied in excess, more wastewater can be efficiently treated.

<Example>

The following examples are intended to illustrate a method of emulsifying and decomposing oil components in waste water, but the present invention is not limited thereto.

Example 1 Microbial Immobilized Carrier Prepared by Direct Heating

Example 1

100 g of 50% caustic soda (Youngjin Chemical Co., Ltd.) and 100 g of oil (Short oil, trade name "Skin Oil" by Shin Dong Bang Co., Ltd.) were mixed and then heated under water bath for 60 minutes to saponify.

Then, the mixture was cooled to 50 ° C and stirred by adding 15 wt% of Tween 20 (Duksan Chemical Co., Ltd.).

Subsequently, Bacillus B4, Bacillus C9, and Candida genus based on the total weight of the prepared support 3 wt% of SY-16 were added and mixed, followed by 9 wt% of the strain coated three times with polysaccharides (100 million bacteria per gram of the sample in terms of bacteria) and mixed again.

Then, 5% by weight of calcium ions were added to prepare a mixed component compound having a size of about 100 g-1 kg, which was poured into a solid mold and immobilized for the solidification time shown in Table 1b to prepare a microorganism immobilization carrier.

The carrier prepared as described above was tested using a ratio of 100 g per ton of wastewater to be treated in a simulated aeration tank, and then measured TOC (total organic compound) and pH for each day of treatment of raw water, untreated water and treated water. Is shown in Table 1 below.

Example 1-2

A microorganism-immobilized carrier was prepared in the same manner as in Example 1 except that 50 g of caustic soda and 150 g of oil (1: 4 weight ratio) were changed.

The carrier prepared as described above was tested using a ratio of 100 g per ton of wastewater to be treated in a simulated aeration tank, and then TOC and pH of the treated water were measured, and the results are shown in Table 1 below.

Example 2 Microbial Immobilized Carrier Prepared by Stirring

Example 2-1

A microorganism-immobilized carrier was prepared in the same manner as in Example 1 except that the mixture was stirred without heating to saponify and 8% by weight of calcium ions were used.

The carrier prepared as described above was tested using a ratio of 100 g per ton of wastewater to be treated in a simulated aeration tank, and then the TOC and pH of the treated water were measured, and the results are shown in Table 1 below.

Example 2-2

A microorganism immobilization carrier was prepared in the same manner as in Example 2 except that 50 g of caustic soda and 150 g of oil were mixed and mixed, and 30 wt% of Tween 20 was used.

The carrier prepared as described above was tested using a ratio of 100 g per ton of wastewater to be treated in a simulated aeration tank, and then TOC and pH of the treated water were measured, and the results are shown in Table 1 below.

Example 3-A method for preparing a microorganism immobilization carrier by adding a microbial blend to a starch, peel, diatomaceous earth mixture

Example 3-1

Dissolve 100 g of starch (manufactured by Duksan Chemical Co., Ltd.) in 100 g of water, heat it, dissolve it, mix 100 g of degreasing steel (dermal skin) (for feed) and 30 g of diatomaceous earth (manufactured by Duksan Chemical Co., Ltd.) and mix it with 100 mesh, then mix and mix. After cooling to 15 wt% of Tween 20 (Duksan Chemical Co., Ltd.) was added and stirred.

Subsequently, Bacillus B4, Bacillus C9, and Candida genus based on the total weight of the prepared support 3 wt% of SY-16 was added and mixed well. Thereafter, 5% by weight of calcium ions were added to prepare a mixed component compound having a size of about 100 g-1 kg, and poured into a solid mold to prepare a microorganism immobilization carrier.

The carrier prepared as described above was tested using a ratio of 100 g per ton of wastewater to be treated in a simulated aeration tank, and then the TOC and pH of the treated water were measured, and the results are shown in Table 1 below.

Example 3-2

Except for changing the starch amount to 50g, the same experiment as in Example 3 was repeated to prepare a microorganism immobilization carrier.

The carrier prepared as described above was tested using a ratio of 100 g per ton of wastewater to be treated in a simulated aeration tank, and then TOC and pH of the treated water were measured, and the results are shown in Table 1 below.

Example 3-3

The same experiment as in Example 3-2 was repeated except that the amount of starch was changed to 150 g, and the TOC and pH thereof are shown in Table 1 below.

TOC (ppm) pH enemy 43,500 6.6 No treatment 1 day 40,100 6.5 3 days 34,300 6.5 5 days 28,100 6.3 7 days 23,600 6.3

Soap composition Mineral salt Solidification time Time to dissolve 100g of final carrier Processing days TOC (ppm) pH Example 1-1 Caustic Soda 100g + Oil 100g Calcium ion 5% by weight 30 minutes 30 days One 32,400 7.1 3 16,700 7.5 5 2,400 7.8 7 700 7.8 Example 1-2 Caustic Soda 50g + Oil 150g " 60 minutes 25 days One 33,400 7.1 3 20.200 7.6 5 5,800 7.9 7 300 8.0 Example 2-1 Caustic Soda 100g + Oil 100g Calcium ion 8 wt% 30 hours 35 days One 33,200 7.2 3 17,200 7.5 5 1,800 7.7 7 400 8.1 Example 2-2 Caustic Soda 50g + Oil 150g " 60 hours 30 days One 31,000 7.3 3 18,000 7.6 5 8,000 7.9 7 600 8.2 Example 3-1 Water 100g + Starch 100g + Degreasing Steel 100g + Diatomaceous Earth 30g Calcium ion 5% by weight 24 hours - One 40,300 6.6 3 21,800 6.4 5 12,600 6.4 7 5,400 6.5 Example 3-2 Water 100g + Starch 50g + Degreasing Steel 100g + Diatomaceous Earth 30g " 30 hours 14 days One 41,200 6.7 3 24,300 6.5 5 10,600 6.4 7 4,100 6.4 Example 3-3 Water 100g + Starch 150g + Degreasing Steel 100g + Diatomaceous Earth 30g " 20 hours 40 days One 41,900 6.7 3 26,700 6.6 5 18,300 6.6 7 9,100 6.5

As can be seen in Table 1, as time passes, the total organic compound (TOC) value can be seen to be greatly reduced compared to raw water, and thus the solid component and the emulsifier are released while the solid component is gradually dissolved, thereby emulsifying the oil. In addition, it can be confirmed that the oil-decomposing microorganisms immobilized in solids emulsify and decompose oil.

It can also be inferred that not only oils but also toxic or hardly degradable substances such as phenols can be effectively treated and can be used to purify large amounts of oil-containing wastewater.

The microorganism immobilization carrier prepared by the present invention is environmentally friendly and mixed with water to components that are difficult to decompose directly in wastewater because it uses a substance which can be easily used for a long time and is economical as well as easily decomposed in nature by a single dose. Possible emulsification may be imparted to aid in effective degradation.

Claims (4)

Caustic soda and oil are mixed and heated to prepare a saponified compound, To 28 to 85% by weight of the saponified compound prepared, 5-30% by weight of an emulsifier is added and stirred, 5 to 27% by weight of microorganisms of Bacillus sp B4, Bacillus sp C9 and Candida sp. , Add 5-15 wt% of mineral salt and mix Consisting of solidifying and manufacturing, Method for preparing microorganism immobilization carrier for wastewater purification. The method of claim 1, wherein the mixing ratio of caustic soda and oil is 1: 1 to 1: 4 weight ratio. The mixture was prepared by mixing 100 g of water in a ratio of 50 to 150 g of starch, heating and dissolving it in a ratio of 100 g of degreasing steel and 30 g of diatomaceous earth. To 28 to 85% by weight of the prepared mixture, 5-30% by weight of an emulsifier is added and stirred, 5 to 27% by weight of microorganisms of Bacillus sp B4, Bacillus sp C9 and Candida sp. , Mix 5 to 15% by weight mineral salt, Consisting of solidifying and manufacturing, Method for preparing microorganism immobilization carrier for wastewater purification. A microorganism immobilization carrier prepared by the method of claim 1.