KR100355952B1 - Skin Care Composition Containing Fish Cartilage Extract and Polypeptide Phytonadione - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a shark cartilage extract and vitamin K derivatives, which is excellent in improving spider vein, a symptom that appears on the surface of the capillary tube, and has excellent safety and stability as a cosmetic. It relates to a cosmetic composition.

Specifically, the present invention contains a polypeptide phytonadione (polypeptide phytonadione) instead of vitamin K to overcome the stability problem of vitamin K to improve the skin improvement effect, safety and stability as a cosmetic (Safety) and stability (Stability) The cosmetic composition which heightened) is related.

Shark cartilage extract in the present invention is contained in an amount of 0.01 to 20.0% by weight, preferably 0.1 to 10.0% by weight relative to the total weight of the composition, the polypeptide K derivative polypeptide phytonadione (Polypeptide phytonadione) to the total weight of the composition It is contained in an amount of 0.0001 to 10.0% by weight, preferably 0.001 to 5.0% by weight.

Description

Skin Care Composition Containing Fish Cartilage Extract and Polypeptide Phytonadione}

The present invention relates to a cosmetic composition containing a shark cartilage extract and a vitamin K derivative, and relates to a cosmetic composition having excellent spider vein improvement effect, which is a symptom that blood vessels appear on the face surface by containing a shark cartilage extract and a vitamin K derivative. Specifically, the present invention relates to a cosmetic composition having excellent skin improvement effect by containing vitamin K derivatives instead of vitamin K, thereby overcoming the stability problem of vitamin K, and improving safety and stability as a cosmetic.

The skin is the largest organ of the human body with an area of 1.8 m ² , the function of a barrier, temperature control, excretion, respiratory function, which protects other important organs of the human body from the outside and prevents the useful components from coming out from the outside It is a very important institution with various roles. However, as the skin ages, the function of the skin is drastically deteriorated, which is accompanied by various changes. In addition, unlike other organs of the human body, the skin is in direct contact with the outside, causing various skin lesions under the direct influence of the external environment, such as UV, drying, environmental pollution. In particular, the skin is very thin and extremely sensitive to changes in the external environment, so when an abnormal phenomenon occurs, the symptoms appear directly on the skin. Spider Vein, a symptom of capillary expansion on the face surface, is caused by the hydrolysis of extracellular matrix surrounding the dermal capillaries by MMP-2 to expand blood vessels. In order to prevent the expansion of blood vessels by inhibiting the activity of MMP-2 that degrades the extracellular matrix or promote blood clotting.

An object of the present invention is to solve the above problems, while effectively improving the spider vein, a symptom that appears on the surface of the capillary tube, while improving the safety and stability of the cosmetic composition vitamin K had a cosmetic composition It is to provide.

To this end, the present invention provides a cosmetic composition that can effectively improve Spider Vein by containing a shark cartilage extract that effectively inhibits the activity of MMP-2, an enzyme that hydrolyzes extracellular matrix, and a vitamin K derivative that promotes blood coagulation. It consists of doing

In particular, in the present invention, by containing a vitamin K derivative instead of using vitamin K, it was possible to overcome safety problems such as skin toxicity and cosmetic cosmetics such as changes over time.

The present invention relates to a cosmetic composition containing a shark cartilage extract and vitamin K derivatives, which is excellent in improving spider vein, a symptom that appears on the surface of the capillary tube, and has excellent safety and stability as a cosmetic. It relates to a cosmetic composition.

Shark cartilage extract of the present invention is an extracellular matrix (Matrix), such as collagen, elastin, prothioglycan, fibronectin, which is important for forming skin connective tissue, from proteolytic enzymes such as collagenase activated by ultraviolet light It is an enzyme inhibitor that inhibits the destruction of connective tissue by protecting. When the skin is exposed to excessive ultraviolet rays, various proteolytic enzymes are expressed in the skin. Among these, proteolytic enzyme called matrix-metalloproteinase (MMP) is collagen, a protein constituting the dermal and dermal-epidermal junction. Intensive breakdown of the skin dramatically reduces the physiological activity of the skin. In particular, when ultraviolet rays are irradiated to the skin, MMP-2 and MMP-9, which are expressed in keratinocytes, selectively degrade collagen Type IV, thereby weakening the dermal-epidermal junction and causing wrinkles. And symptoms of sagging skin due to a decrease in elasticity. The skin contains an enzyme inhibitor called TIMP-1 (Tissue Inhibition Metalloproteinase-1), which can inhibit the damage of skin components caused by MMP-2.However, if the activity of TIMP-1 rapidly decreases with age, The degradation of collagen is markedly increased. Spider Vein, a symptom of capillary expansion on the surface of a spider's face, is caused by the hydrolysis of extracellular matrix surrounding the dermal capillaries by MMP-2 to expand blood vessels. Shark cartilage extract effectively inhibits the activity of MMP-2, thereby reducing Spider Vein in the skin. In addition, the overproduction and activity of proteolytic enzymes such as collagenase may cause abnormal skin reactions, such as inflammation, shark cartilage extract effectively inhibits such skin irritation.

Vitamin K plays an important role in the growth and development of the human body, especially fat-soluble vitamins that are involved in the prevention of bleeding as an important factor of blood coagulation is accumulated in the body's adipose tissue. There are three kinds of vitamin K. Among them, vitamin K1 (Phytonadione), which is found in nature and is found in many plants, is composed of side chains of Nophoquinone and Isoprene units, and the molecular formula is C ₃₁ H ₄₆ O ₂ . Vitamin K2 (Menaquinone) can be obtained from naturally occurring bacteria in the intestine, and vitamin K3 (Menadione) is obtained by synthesizing such a problem of vitamin K as a cosmetics, which is less stable and slightly toxic in the skin, There is difficulty in penetration. The vitamin K derivative used in the present invention is obtained by reacting pure vitamin K1 with yeast, and the protein is bound to vitamin K1 from this reaction. The obtained vitamin K derivative is polypeptide phytonadione, which increases the stability of vitamin K, reduces toxicity, and facilitates skin penetration by polypeptide. Vitamin K has the effect of reducing the invisible Spider vein, the ability to quickly heal bruises, and reduce the erythema and inflammation.

In order to achieve the desired effect in the present invention, the shark cartilage extract is contained in an amount of 0.01 to 20.0% by weight, preferably 0.1 to 10.0% by weight based on the total weight of the composition, and a polypeptide K-derived polypeptide phytonadione (Polypeptide) phytonadione) is contained in an amount of 0.0001 to 10.0% by weight, preferably 0.001 to 5.0% by weight based on the total weight of the composition.

The cosmetic composition containing the shark cartilage extract of the present invention and the polypeptide phytonadione which is a vitamin K derivative is not particularly limited in the formulation, for example, softening water, astringent makeup, nutrient cream, eye cream, nutrition cream It may have a formulation such as, massage cream, cleansing cream, cleansing foam, cleansing water, powder, essence, pack.

In addition, in the cosmetic composition of each formulation, the components other than the above-mentioned shark cartilage extract and the vitamin K derivative polypeptide phytonadione can be appropriately selected and blended by the person without difficulty according to the formulation or purpose of use of other cosmetics. .

Hereinafter, the configuration and effects of the present invention will be described in detail with reference to test examples. However, the present invention is not intended to be limited to these test examples.

For the test example, as shown in Table 1, the composition containing the shark cartilage extract of the present invention and the polypeptide phytopolypeptide pythonadione (example), shark cartilage extract and the vitamin K derivative polypeptide phytonadione any of A composition (Comparative Example 1) containing no shark cartilage extract and vitamin K (Comparative Example 2) were prepared.

Examples and Comparative Examples Unit (part by weight) Ingredient Example Comparative Example 1 Comparative Example 2 Shark Cartilage Extract 3.0 - 3.0 Polypeptide Phytonadione 1.0 - - Vitamin K - - 0.1 vaseline 7.0 7.0 7.0 Liquid paraffin 10.0 10.0 10.0 Beeswax 2.0 2.0 2.0 Polysorbate 60 2.0 2.0 2.0 Sorbitan sesquioleate 2.5 2.5 2.5 Squalane 3.0 3.0 3.0 Propylene glycol 6.0 6.0 6.0 glycerin 4.0 4.0 4.0 Triethanolamine 0.5 0.5 0.5 Carboxy Vinyl Polymer 0.5 0.5 0.5 Tocopheryl Acetate 0.1 0.1 0.1 Incense, preservative a very small amount a very small amount a very small amount Purified water to 100 to 100 to 100

Test Example 1 Spider vein reduction effect

Spider vein reduction effect on the shark cartilage extract and the cosmetic containing polypeptide phytonadione was measured.

Twenty women over 20 years old with distinct blood vessels but no capillary dilatation were divided into two groups. Examples in Group A and Comparative Example 1 in Group B were formulated according to Table 1. The film was applied to the entire surface, and four spots were selected from the surface of the face where blood vessels such as spider webs were observed, and observed with a fiber optic surface microscope. The images were analyzed for gray values using a Zeiss Ibas image analyzer, and the integrated optical density (IOD) was measured at four-week intervals (T0, T4, T8, and T12), and the results are shown in Table 2 below.

% Reduction of spider vein Skin wrinkle improvement Example Comparative Example 1 T0 T4 T8 T12 T0 T4 T8 T12 Subject 1 0 5 23 31 Subject 11 0 2 2 One Subject 2 0 2 18 36 Subject 12 0 0 One 3 Subject 3 0 7 32 55 Subject 13 0 -3 2 0 Subject 4 0 One 20 29 Subject 14 0 One One 3 Subject 5 0 10 16 37 Subject 15 0 0 2 2 Subject 6 0 15 36 62 Subject 16 0 -5 One -One Subject 7 0 5 32 45 Subject 17 0 One One 2 Subject 8 0 3 11 21 Subject 18 0 0 2 One Subject 9 0 9 28 34 Subject 19 0 One 2 5 Subject 10 0 2 26 49 Subject 20 0 2 2 5 ΔR ( T12 -T0) = 39.9 ΔR ( T12 -T0) = 2.1

As a result, in the case of Example, all the subjects showed significant spider vein reduction effect (△ R ( T12-T0) = 39.9, p <0.01), indicating that shark cartilage extract and polypeptide phytonadione are very effective in reducing spider vein. Can be. In particular, after about two months, the effect began to manifest.

Test Example 2 Moisturizing Effect

The skin moisturizing effect on the shark cartilage extract and the cosmetic containing polypeptide phytonadione were measured.

After dividing 20 healthy women into two groups (A, B) at 25 ° C, 45% relative humidity, and no air flow, compare the examples to group A and group B Example 1 was formulated according to Table 1 above and applied to the upper arm of the arm three times a day for 6 weeks, using TEWAMETER TM210 (C + K electronic GmbH. Germany) transdermal moisture loss, that is, TEWL (Transepidermal Water Loss) The value is measured by the change of TEWL value with time △ TEWL, and the moisture content of the skin is quantified from 0 to 150 by the change of electrical conductivity according to the moisture content of the epidermis using CORNEOMETER CM 820 PC (C + K electronic GmbH. Germany). Moisturizing effect was measured. The experimental results are shown in Table 3 below.

Skin moisturizing effect Test Product △ TEWL Corneometer value Example 6.5 120 Comparative Example 1 1.5 85

As a result, the ΔTEWL value of the cream containing the shark cartilage extract and the polypeptide phytonadione (Example) was 6.5 g / hm ² , which shows that the percutaneous moisture loss was significantly improved after 6 weeks compared to the comparative example. .

In addition, in the Corneometer value measured the moisture content of the skin, the embodiment shows a higher increase than the comparative example, it can be seen that shark cartilage extract and polypeptide phytonadione greatly enhances the moisturizing effect.

Test Example 3 Skin Safety Test

Skin safety test for the cosmetic containing the shark cartilage extract and polypeptide phytonadione was carried out.

Forty-five subjects (mean age 20, age 19-32) were divided into three groups and the patch test was performed on the upper arm using Haye's Test Chamber. Psoriasis, eczema, and other skin lesion holders, or those who are pregnant, nursing or contraceptives, and antihistamines, were excluded from this study.

Wipe the test site with 70% ethanol and dry it and then formulated according to Table 1 above (containing shark cartilage extract and polypeptide phytonadione), Comparative Example 1 (shark cartilage extract and polypeptide phytonadione mi) ), Comparative Example 2 (containing shark cartilage extract and 0.01% pure vitamin K1 (phytonadione)) in Example A, Comparative Example 1 in Group B, and Comparative Example 2 in Group C, respectively. After dropping into the chamber by ㎍, fix it on the upper part of the test site. Apply the patch for 24 hours and after removing the patch, mark the test site with a marking pen and observe the test site after 24 hours and 48 hours, respectively. Determination was performed 24 hours and 48 hours after patch removal, and skin reaction was determined according to the regulations of the International Contact Dermatitis Research Group (ICDRG) shown in Table 4 below. The experimental results are shown in Table 5 below.

Criteria for international contact dermatitis sign Criteria evaluation Mean score ± ++++++- Doubtful or slight reaction and erythemaErythema + IndurationErythema + Induration + VesicleErythema + Induration + Bullae Stimulus stimulus non stimulus 0 to 0.91.0 to 2.93.0 to 4.95.0 or more

Skin safety Elapsed time Example Comparative Example 1 Comparative Example 2 24 hours 0 1.5 2.5 48 hours 0 0.5 1.5

As a result of skin safety test, the cream containing shark cartilage extract and polypeptide phytonadione (Example) had a mean stimulus of 0 and showed no irritation in all the subjects. Not only is it a safe substance, it can be used to relieve skin irritation such as inflammation. On the other hand, Comparative Example 2 containing pure vitamin K1 is shown as a light stimulus, it can be seen that pure vitamin K1 is a substance having a skin irritation.

Test Example 4 Stability Test

The stability test of the formulation for the cosmetic containing the shark cartilage extract and polypeptide phytonadione was carried out.

In order to test the stability of the cosmetics Examples of Table 1 (including shark cartilage extract and polypeptide phytonadione), Comparative Example 1 (shark-free cartilage extract and polypeptide phytonadione), Comparative Example 2 (shark) Cartilage extract and 0.01% pure vitamin K1 (phytonadione)) are stored in an opaque glass container for 12 weeks in a constant temperature bath at 45 ° C and stored in a completely shaded refrigerator kept at 4 ° C. After storing for 12 weeks in an opaque glass container, the degree of separation and discoloration were measured. The results are shown in Table 6 below. At this time, the degree of product separation and discoloration were evaluated by classifying into the following six grades.

Product discoloration evaluation criteria:

0: no change 1: very little separation (discoloration)

2: Separate slightly (discolored) 3: Separate slightly (discolored)

4: severely separated (discolored) 5: extremely severely separated (discolored)

Separation and discoloration Temperature Discoloration degree of test substance Example Comparative Example 1 Comparative Example 2 45 ℃ 0 1.5 3.2 4 ℃ 0 0 0.5

As can be seen in Table 6, the cream containing the shark cartilage extract of the present invention and the polypeptide phytonadione (example) can be seen that there is no discoloration and separation phenomenon is a stable base. On the other hand, Comparative Example 2 containing the shark cartilage extract and phytonadione, a pure vitamin K1, shows a severe discoloration phenomenon, which is very unstable.

From the results of the above test results, it can be seen that the cosmetic containing shark cartilage extract and polypeptide phytonadione not only effectively reduces spider vein but also has excellent skin moisturizing effect. It can be seen that vitamin K can be applied as a cosmetic by solving the problem of stability with a vitamin K derivative.

Hereinafter, based on the results of the test examples described above, a cosmetic composition of the present invention containing shark cartilage extract and polypeptide phytonadione is presented. However, the composition of the present invention is not intended to be limited to the following formulation examples.

Formulation Example 1 Softening Cosmetic (Skin Lotion)

Ingredient Parts by weight Shark Cartilage Extract 0.1 Polypeptide Phytonadione 0.05 1,3-butylene glycol 6.0 glycerin 4.0 Oleyl alcohol 0.1 Polysorbate 20 0.5 ethanol 15.0 Benzophenone-9 0.05 Incense, preservative a very small amount Purified water to 100

Formulation Example 2. Nutrients (Milk Lotion)

Ingredient Parts by weight Shark Cartilage Extract 1.0 Polypeptide Phytonadione 0.2 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 1.2 Tocopheryl Acetate 3.0 Floating paraffin 5.0 Squalane 3.0 Macadamia Nut Oil 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.0 Carboxyvinyl Polymer 1.0 Incense, preservative a very small amount Purified water to 100

Formulation Example 3. Nutrition Cream

Ingredient Parts by weight Shark Cartilage Extract 3.0 Polypeptide Phytonadione 1.0 vaseline 7.0 Liquid paraffin 10.0 Beeswax 2.0 Polysorbate 60 2.0 Sorbitan sesquioleate 2.5 Squalane 3.0 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 0.5 Carboxy Vinyl Polymer 0.5 Tocopheryl Acetate 0.1 Incense, preservative a very small amount Purified water to 100

Formulation Example 4 Massage Cream

Ingredient Parts by weight Shark Cartilage Extract 0.1 Polypeptide Phytonadione 0.1 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 0.5 Beeswax 2.0 Tocopheryl Acetate 0.1 Polysorbate 60 3.0 Sorbitan sesquioleate 2.5 Cetearyl Alcohol 2.0 Liquid paraffin 30.0 Carboxy Vinyl Polymer 0.5 Incense, preservative a very small amount Purified water to 100

Formulation Example 5 Pack

Ingredient Parts by weight Shark Cartilage Extract 3.0 Polypeptide Phytonadione 0.2 Propylene glycol 2.0 glycerin 4.0 Carboxyvinyl Polymer 0.3 ethanol 7.0 Fiji-40 Hydrogenated Castor Oil 0.8 Triethanolamine 0.3 Incense, preservative a very small amount Purified water to 100

As described above, the cosmetic composition of the present invention containing the shark cartilage extract and the polypeptide phytonadione is completed, thereby effectively reducing spider vein and providing a cosmetic composition having excellent skin moisturizing effect.

In addition, by containing a polypeptide phytopionedione, a vitamin K derivative instead of vitamin K, vitamin K can be applied as a cosmetic by solving problems of skin safety and formulation stability of vitamin K.

Claims (4)

A skin care cosmetic composition comprising a shark cartilage extract and a polypeptide phytonadione which is a vitamin K derivative, a skin care cosmetic composition The method of claim 1, Shark cartilage extract is contained in an amount of 0.01 to 20.0% by weight of the total composition, the vitamin K derivative polypeptide phytonadione (Polypeptide phytonadione) is contained in an amount of 0.0001 to 10.0% by weight relative to the total weight of the composition , Skin protection cosmetic composition. The method according to claim 1 or 2, Shark cartilage extract is contained in an amount of 0.1 to 10.0% by weight based on the total weight of the composition, and the vitamin K derivative polypeptide phytonadione (Polypeptide phytonadione) is contained in an amount of 0.001 to 5.0% by weight relative to the total weight of the composition Skin protection cosmetic composition. The method of claim 1, Polypeptide phytonadione is a vitamin K derivative, Polypeptide phytonadione is a skin protection cosmetic composition, characterized in that produced by reacting the pure vitamin K1 and yeast (yeast).