KR100351548B1 - Conditioning hair-care containing fermented product using lactic acid bacteria - Google Patents

Abstract

The present invention is lactic acid bacteria fermentation broth of Korean lactic acid bacteria Bifido bacteria Rongum; Or a group consisting of Korean lactic acid bacteria Bifido bacteria Rongum, Lactobacillus casei (KFCC 10813), Lactobacillus acidophilus (KCTC 3170) and Streptococcus thermophilus (ATCC 19987) The present invention relates to a hair cosmetic composition containing a complex lactic acid bacteria fermentation broth of at least one strain selected from the present invention, wherein the hair cosmetic composition containing the lactic acid bacteria fermentation broth of the present invention increases the moisture content of the hair to impart gloss to the hair and gives a soft texture. It is excellent in preventing hair splitting and damage.

Description

Hair cosmetics containing lactic acid bacteria fermentation broth {CONDITIONING HAIR-CARE CONTAINING FERMENTED PRODUCT USING LACTIC ACID BACTERIA}

The present invention relates to a hair cosmetic containing a high-functional lactic acid bacteria fermentation broth that enhances the water retention ability of the hair and imparts a conditioning effect and a soft feel to the hair.

The hair cuticle (MoSophie) layer is bonded to each other by an epicuticle layer having hydrophobic and hard properties, and an endocuticle layer having a high affinity for water and an exocuticle and a cell membrane complex (CMC). Moisture adsorption of the hair is largely involved in hydrophilic side chain portions and peptide bonds such as amino groups, guanizino groups and carbonyl groups of keratin fibers constituting the hair.

When water is moisturized, the water first penetrates the entire cuticle around the exocuticle, which has high hydrophilicity, and penetrates into the cortex inside the hair through the cell membrane complex.

In addition, water molecules are presumed to be adsorbed by hydrophilic side chains of keratin fibers in the matrix, hydrogen bonds, and cholesterol in hair internal lipids, and water is retained. Moisture inside is considered to be protected from evaporation by barrier walls called cuticles, but the exact mechanism by which hair retains moisture is still difficult to clarify.

In order to maintain the moisture of the hair, a moisturizer having a high moisturizing effect on the inside or the surface of the hair should be used, or a film having a high inhibitory effect on moisture transpiration should be formed on the surface of the hair. Vegetable protein hydrolysates such as derivatives and soybeans have been used.

In general, hair damage can be classified into physical damage caused by washing, brushing, dryer, heating, and chemical damage caused by dyeing, bleach, environmental damage caused by ultraviolet rays, heat, and the like. It is easily damaged and degrades, resulting in increased dryness and untidy or cracked and damaged hair, and reduced strength.

Therefore, in order to alleviate and protect such hair damage and at the same time give and restore a soft feel to the hair, hair cosmetics generally include silicone resins (Korean Patent Publication No. 1999-231309), water-insoluble synthetic esters and hydrocarbon oils, Fatty acid esters and mixtures thereof (Korean Patent Publication No. 2000-50266), Insoluble Silicones and Cationic Polymers (Korean Patent Publication No. 1999-007953), Anionic Cleaning Surfactants and Insoluble Silicones (US Pat. Nos. 4,741,855, 4,788,066) At least one hydrophilic moiety containing at least one hydrophilic moiety selected from 2-hydroxyalkanoic acid (Korean Patent Publication No. 95-3085), alkoxy, polyoxyalkylene, alkylamido, hydroxy alkyl, alkyl ester residues and mixtures thereof Quaternary ammonium or amino compounds having N-radicals (Korean Patent Publication No. 93-702955) and the like are used, and a shampoo composition having a moisturizing effect Oligo chitosan obtained by partial hydrolysis of chitosan to obtain water (Korean Patent Publication No. 98-046484), side chain alkyl quaternary ammonium salts to impart an antistatic effect and certain oil compounds (Korean Patent Publication No. 95-7904) ), And other copolymers of hydrophilic unsaturated monomers with polysiloxane groups (Korean Patent Publication No. 98-148482) are also used, and hair cosmetics containing ester oil and alkyl polyglucoside together (Korean Patent Publication No. 99-203734). ) Also provides good conditioning effect for hair and gives hair shine and flexibility.

However, conventional hair cosmetics as described above are cationic surfactants such as cetyltriethylammonium chloride, cationic polymers, for example, in order to obtain conditioning effects such as smoothness, usability, and finish when drying the hair. Polyvinylpyridinium, quaternized silicones such as trimethyl silal amodimethicone, high molecular weight dimethyl polysiloxanes, amino modified silicones, volatile silicones such as cyclomethicone, It has been difficult to enhance the moisture retention of hair based on the deposition of functional ingredients into hair fibers.

In addition, since the adsorption amount of functional components on the hair varies depending on the isoelectric point, which exhibits acid and base properties on the surface of the hair, it is difficult to maintain hair conditioning even after rinsing.

On the other hand, the present inventors have found that the skin cosmetics containing the complex lactic acid bacteria fermentation broth have the effect of increasing the skin's moisturizing power, reducing wrinkles, and whitening effect (Korean Unexamined Patent Application No. 98-54944). The present invention was completed by confirming that the combined lactic acid bacteria culture drug of the combination exhibited excellent effects in hair coordination.

Accordingly, an object of the present invention is to solve the above problems of the conventional hair cosmetics, and to provide a new hair cosmetics having improved hair-conditioning ability and improved hair conditioning effect and flexibility.

1 is a graph showing Combing Force vs. load curve of hair treated with the samples of Examples and Comparative Examples of the present invention according to Test Example 1. FIG.

To this end, according to the present invention, lactic acid bacteria fermentation broth of Korean type lactic acid bacteria Bifidobacteria longum (KFCC 10870); Or Bifidobacteria longum (KFCC 10870), Lactobacillus casei (KFCC 10813), Lactobacillus acidophilus (KCTC 3170) and Streptococcus thermophilus (phileptococcus thermo) ATCC 19987) is provided a hair cosmetic containing a complex lactic acid bacteria fermentation broth of one or more strains selected from the group consisting of.

Hereinafter, the content of the present invention in more detail as follows.

The present inventors focused on the point that the isoelectric point of hair is pH 4.5 to 5.5 as described above, and as a result of verifying the efficacy and effect of many lactic acid bacteria fermentation broths, Korean lactic acid bacteria Bifidobacteria longum (KFCC 10870), Lactobacillus ka Lactobacillus casei (KFCC 10813), Lactobacillus acidophilus (KCTC 3170) and Streptococcus thermophilus (ATCC 19987) have been found to be useful, especially Korean Lactobacillus Bifidobacteria L. It was confirmed that the lactic acid bacterium fermentation broth obtained by complex culturing of two or more bacteria further enhances the moisture retention ability of the hair to give the hair conditioning effect and a soft feel.

Bifidobacteria Rongum (KFCC 10870) used in the present invention is a bacterium isolated from Korean intestines and has excellent lactic acid production ability and vitamin B group production ability, which is applied to the production of foods such as yogurt, and applied to some skin cosmetics. It is known to have an effect on the whitening effect and skin moisturizing, active oxygen removal ability, but the provision of the conditioning effect to the hair cosmetics and the enhancement of the moisture retention ability of the hair, hair treatment effect was first confirmed by the inventors of the present application.

Lactobacillus casei has been used mainly in dairy production and is known to have the highest titer.

Lactobacillus ashdophyllus has been used in the manufacture of food, beverages and some cosmetics, and is known for its ability to inhibit the growth of other microorganisms.

Streptococcus thermophilus is a thermophilic bacterium and has excellent lactic acid production ability, and has been used in cosmetic compositions for nutritional moisturizing as well as food and beverage.

In the present invention, at least one bacterium selected from the group consisting of Korean Bifidobacterium Rongum alone or Korean Bifidobacterium Rongum, and Lactobacillus casei, Lactobacillus ashidophyllus and Straptococcus thermophilus, in particular four species Can be cultured by inoculating a conventionally used lactic acid bacteria medium, such as a skim milk containing medium or a whey containing medium.

For example, Scheme Milk, 10 (W / V)% Whey 90 (Whey 90; 90% Mineral), 5 (W / V)% Whey 50 (50% Mineral), 5 (W / V)% whey 90 (90% mineral removed) medium may be used, with 5-15 (W / V)% skim milk containing medium being particularly preferred.

The pH of the culture solution is preferably maintained at 7.0 to 7.4, and the culture is preferably carried out while maintaining the temperature of 36 to 37 ° C at the time of culturing and the condition of 1 VVM of aeration amount.

When the obtained fermentation broth is centrifuged to remove the polymer precipitate and the microbial cells, the lactic acid bacteria fermentation broth of the present invention having excellent hair moisture, retention ability enhancement effect, conditioning effect improvement and hair flexibility improvement effect can be obtained.

Lactic acid bacteria fermentation broth according to the present invention can be added to hair cosmetics, in particular shampoo, rinse, hair cream, hair lotion, etc., the amount is preferably contained in an amount of 0.01 to 20% by weight based on the total weight of the cosmetic composition. The effect cannot be expected at 0.01 wt% or less, and the increase in effect due to the increase of the content is less than 20 wt%, resulting in low economic efficiency.

Hereinafter, the present invention will be described by Examples, Preparation Examples, and Comparative Examples. The following examples are provided only to facilitate understanding of the present invention, the present invention is not limited to these examples, production examples.

Example 1

Korean Lactobacillus Bifidobacteria longum (KFCC 10870), Lactobacillus casei (KFCC 10813), Lactobacillus acidophilus (KCTC 3170), Streptococcus thermophilus; 4 microorganisms in 19987) at the same concentration (10 ⁶ cells / ml), bactotriptone 1.0% (W / V), bactopeptone 0.5% (W / V), skim milk 10% (W / V), Tris 30 ml inoculation (7.5 ml each) was inoculated in a buffered 3 L culture medium, and cultured for 6 hours while maintaining a hydrogen ion concentration (pH) of 7.0 to 7.4, a temperature of 36 to 37 ° C, and an aeration of 1 VVM. At 6 hours, 60% glucose solution and 10% peptone solution prepared in advance were supplied to maintain the glucose concentration in the culture solution at 6% and the proteose concentration at 1%. After incubation for 24 hours, the culture was terminated to obtain a fermentation broth. 6,000 g of the fermentation broth was warmed at 70 ° C. for 30 minutes and centrifuged at 1,000 rpm for 30 minutes to obtain 1,500 g of lactic acid bacteria fermentation broth from which polymer precipitates and lactic acid bacteria were removed.

Example 2

Except for using Korean Lactobacillus Bifidobacteria longum (KFCC 10870) and Streptococcus thermophilus (ATCC 19987) at a total concentration of 10 ⁶ cells / ml at a total concentration of 30 ml of 30 ml each, Example 1 Incubated and treated under the same conditions as 1,500 g of lactic acid bacteria fermentation broth.

Example 3

A lactic acid bacterium fermentation broth 1,500 g was obtained in the same manner as in Example 1, except that only Korean lactic acid bacteria Bifidobacteria longum (KFCC 10870) was used in 30 ml at a concentration of 10 ⁶ cells / ml.

Preparation Example 1, 2, 3 and Comparative Example 1

Manufacture of shampoo

A hair treatment shampoo composition was prepared containing or not containing lactic acid bacteria fermentation broths prepared according to Examples 1, 2 and 3, respectively (Comparative Examples 1, 2 and 3). The composition (Table 1) and the manufacturing method are as follows.

After dissolving 1 in 15 components, the components of 2 to 8 were sequentially mixed, heated to 70-80 ° C., cooled to 65 ° C., and 9-14 components were added thereto. The mixture was uniformly mixed and stirred for 10 minutes and then completed.

Preparation Example 4, 5, 6 and Comparative Example 2

Manufacture of hair treatments

Hair treatment compositions containing or not containing the composite lactic acid bacteria fermentation broths prepared according to Examples 1, 2 and 3 (Preparation Examples 4, 5 and 6) (Comparative Example 2) were prepared. The composition (Table 2) and the production method are as follows.

The components of 6 to 9 were evenly dispersed in the components of 12 and heated. While stirring, heated 1 to 5 homogeneous solutions were added to the dispersion and allowed to cool, then 10 and 11 were added and stirred for 10 minutes to prepare a hair treatment.

Preparation Example 7, 8, 9 and Comparative Example 3

Preparation of Hair Lotion

Hair lotion compositions containing or not containing the composite lactic acid bacteria fermentation broths prepared according to Examples 1, 2 and 3 (Preparation Examples 7, 8, 9) (Comparative Example 3) were prepared. The composition (Table 3) and the production method are as follows.

The components 1 to 5 were heated to 70 ° C. and stirred uniformly to 9 components, then cooled to 45 ° C., and the ingredients 6, 7 and 8 were added to mix the mixture to prepare a hair lotion.

Preparation Example 10, 11, 12 and Comparative Example 4

Production of hair rinse

A hair rinse composition was prepared with or without the complex lactic acid bacteria fermentation broths prepared according to Examples 1, 2 and 3 (Preparation Examples 10, 11, 12) (Comparative Example 4). The composition (Table 4)) and the production method are as follows.

The component 6 was heated to 70 degreeC, the mixture of 1-3 components heated previously was added, stirred, emulsified, cooled to 45 degreeC, and the components of 4 and 5 were added, and hair rinse was prepared.

Test Example 1

Hair Flexibility Effect Test

Take the same amount (3.00g-12) of Korean hair samples, wash them with lukewarm water at about 40 ° C and completely dry them, and then wash the hair samples with the samples of Preparation Examples 1, 2 and 3 and the sample of Comparative Example 1 After drying, a combed feel gauge designed by Garcia ML [Garcia ML et al, J. Soc. Cosmet. Chem., 27, 379 (1976)] was used to evaluate the flexibility of hair by measuring the force applied when combing (Korean Patent Publication No. 90-6825).

The hair sample was measured and calculated by repeating the end peak and the area of the hair sample three times using an Instron Tester (Metric Table Model, TM-M) in a constant temperature and humidity room.

In this test, the arithmetic mean was measured three times for each production example and comparative example. The smaller the value of the end peak, the smaller the area, the better the flexibility of the hair (Table 5).

Hair Flexibility Test Name: Hair Combing Force

Instrument: Instron Tester

Conditions: 50% relative humidity, 25 ° C temperature, 12 samples of hair amount 3.00g, comb of hard rubber material with comb spacing of 2,5mm, range 10cm, speed 5cm / min.

Comparative Example 1 Preparation Example 1 Preparation Example 2 Preparation Example 3 End Peak (Unit: mN / g Hair) 120 63 82 97 Area (unit: mm2 / g Hair) 195 76 113 134

As shown in the test results of Table 5 above, the samples of Preparation Examples 1, 2, and 3 containing the lactic acid bacteria fermentation broth according to the present invention (particularly, in the case of Preparation Example 1 containing four complex lactic acid bacteria fermentation broths), It was found to be significantly superior in hair flexibility compared to the sample.

In addition, in the same manner as in Test Example 1, when measured with a hair sample of 10.00g, a travel distance of 15cm, a speed of 10cm / min, the results for the load (G) applied to the hair to the combing texture, the results in Comparative Example 1 It is shown in the results of FIG. 1 that the samples of Preparation Examples 1, 2, and 3 are superior to those of FIG. 1 (FIG. 1).

Test Example 2

Affinity with hair (moisture retention test)

Approximately 10.0 g of each hair sample is made out of three pieces and a total of twelve pieces. After washing, spreading and removing water are sufficiently removed, dried for 1 hour in a 60 ° C thermostat, and cooled in a silica gel desiccator to accurately measure the weight of the hair.

After the measurement, these two hair samples were sufficiently wetted with the sample solutions of Preparation Examples 1, 2 and 3 and the sample solution of Comparative Example 1, and then rubbed, followed by washing for 1 minute in running water at about 40 ° C. Subsequently, the sample of Preparation Examples 10, 11, and 12 and the sample solution of Comparative Example 4 were sufficiently wetted and rubbed lightly, and then washed with flowing water before and after 40 ° C for 30 seconds, then spread out to remove sufficient moisture. It dried for 1 hour.

Subsequently, the mixture was allowed to cool in silica gel desiccator, and the weight of the hair was measured precisely, and the affinity with the hair was examined using the difference from the measured weight as the hair adsorption amount (%) of each sample.

Hair adsorption amount (%) =

Preparation Example 1+ Preparation Example 10 Preparation Example 2+ Preparation Example 11 Preparation Example 3+ Preparation Example 12 Comparative Example 1+ Comparative Example 4 Hair adsorption amount (%) 1.40 0.9 0.65 0.15

As can be seen from the above test results, the hair was treated with the shampoo and rinse of the preparation example containing the lactic acid bacteria fermentation broth according to the present invention (in particular, the shampoos and rinses of preparation examples 1 and 10 containing four complex lactic acid fermentation broths). When compared with the hair treated with the sample of the comparative example, the adsorption power is much better, and thus the affinity with the hair was found to be excellent.

Test Example 3

Conditioning effect measurement test

For the comparative evaluation as a cosmetic of the conditioning agent, the combing touch potential, touch (feeling test), gloss, and the like in the wet or dry condition were compared and evaluated. (Korean Patent Publication No. 95-7904)

The panel selects 15 men and women who can detect hair damage and washes them with regular shampoo, and after 2 hours, divides the scalp hair of each subject into two parts, divided into three groups of five people each, and the left part is manufactured in Examples 4 and 5. And to apply the sample of 6, to the right portion was to apply the sample of Comparative Example 2 and to record the feeling.

The evaluation grade was 0-4, and the records were recorded from "0-bad" to "4-very good", and the arithmetic mean of 15 panels was obtained.

Tactile Comb texture potential Polish Preparation Example 4 3.41 3.23 2.90 Preparation Example 5 2.98 2.77 2.53 Preparation Example 6 2.51 2.50 2.36 Comparative Example 2 1.88 1.50 2.04

As can be seen from the above results, hair treated with samples of Preparation Examples 4, 5, and 6 containing the lactic acid bacteria fermentation broth according to the present invention (particularly, in Preparation Example 4 containing four complex lactic acid bacteria fermentation broths) was compared. Compared with the sample of Example 2, it was found to be excellent in touch, comb feel potential, gloss and the like.

According to the present invention, it acts from the area close to the inner side of the hair epidermis to the cortex which occupies most of the hair, thereby increasing the persistence of the moisture content necessary for making the hair moist, and restoring the cuticle of the damaged hair, thereby providing a combing effect. A high hair conditioning cosmetic composition can be obtained.

Claims (2)

Lactic acid bacteria fermentation broth of Korean lactic acid bacteria Bifidobacteria longum (KFCC 10870); Or Bifidobacteria longum (KFCC 10870), Lactobacillus casei (KFCC 10813), Lactobacillus acidophilus (KCTC 3170) and Streptococcus thermophilus (phileptococcus thermo) A hair cosmetic containing a complex lactic acid bacteria fermentation broth of at least one strain selected from the group consisting of ATCC 19987). Hair cosmetic composition containing 0.01 to 20% by weight of the lactic acid bacteria fermentation broth according to claim 1.