KR100350906B1 - 뱀의 독소로부터 분리된 신규한 트롬빈 유사효소 및 그의제조방법 - Google Patents
뱀의 독소로부터 분리된 신규한 트롬빈 유사효소 및 그의제조방법 Download PDFInfo
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- KR100350906B1 KR100350906B1 KR1020000032654A KR20000032654A KR100350906B1 KR 100350906 B1 KR100350906 B1 KR 100350906B1 KR 1020000032654 A KR1020000032654 A KR 1020000032654A KR 20000032654 A KR20000032654 A KR 20000032654A KR 100350906 B1 KR100350906 B1 KR 100350906B1
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Abstract
Description
트롬빈-유사효소 | |||
총 단백질(㎎) | 총 활성(NIHunit)* | 특이활성(NIHunit/㎎) | |
뱀의 조독소(crude venom) | 190 | 2660 | 14 |
Q-세파로즈(Q-Sepharose) | 18.2 | 1750 | 96.2 |
슈퍼덱스 75(Superdex 75) | 3.7 | 772 | 257.3 |
모노-Q FPLC (Mono-Q FPLC) | .062 | 217 | 350 |
Claims (2)
- 다음과 같은 특성을 가지는 세린계 단백분해효소인 트롬빈 유사효소:(ⅰ) 한국산 칠점사(Agkistrodon saxatilis emelianov)의 독소로부터 유래되는 당단백질이며;(ⅱ) IIGGDEXNINEHRFL인 N-말단 아미노산 서열을 포함하고;(ⅲ) SDS-PAGE로 분석시, 분자량이 약 39kD이며;(ⅳ) 펩타이드-N-글리코시다제-F에 의하여 아스파라긴 잔기에 연결된 당이 제거된 후의 분자량은 약 28kD이고;(ⅴ) PMSF 또는 류펩틴에 의하여 효소활성이 억제되며;(ⅵ) 트롬빈 특이성 기질인 D-Phe-Pip-Arg-p-nitroanilide 또는 칼리크레인 특이성 기질인 N-benzoyl-Phe-Val-Arg-p-nitrdanilide을 분해하고, 이들 기질에 대한 Km값은 34 내지 64μM이고;(ⅶ) 효소 활성의 초기 단계에서 피브리노겐 A 사슬을 분해하고, 반응이 연장될 경우 피브리노겐 B 사슬을 분해하며; 및,(ⅷ) 피브린 용해효소에 의하여 용이하게 분해되는 불안정한 피브리노겐 응괴(fibrinogen clotting)를 유도한다.
- 한국산 칠점사(Agkistrodon saxatilis emelianov)의 독소를 음이온 교환 크로마토그래피를 수행하고 겔 여과한 후, 음이온 교환 크로마토그래피를 재수행하는 단계를 포함하는 제 1항의 트롬빈 유사효소를 제조하는 방법.
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KR1020000032654A KR100350906B1 (ko) | 2000-06-14 | 2000-06-14 | 뱀의 독소로부터 분리된 신규한 트롬빈 유사효소 및 그의제조방법 |
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KR1020000032654A KR100350906B1 (ko) | 2000-06-14 | 2000-06-14 | 뱀의 독소로부터 분리된 신규한 트롬빈 유사효소 및 그의제조방법 |
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KR20010111891A KR20010111891A (ko) | 2001-12-20 |
KR100350906B1 true KR100350906B1 (ko) | 2002-09-05 |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20220146149A (ko) | 2021-04-23 | 2022-11-01 | 전남대학교산학협력단 | 한국형 살무사과 사독 감별용 조성물 |
KR20220170290A (ko) | 2021-06-22 | 2022-12-29 | 전남대학교산학협력단 | 한국형 살무사과 사독의 항사독 조성물 |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1324132C (zh) * | 2004-10-19 | 2007-07-04 | 北京大学 | 一种蛇毒类凝血酶及其编码基因与应用 |
JP7393006B2 (ja) * | 2018-01-25 | 2023-12-06 | ペンタファーム アーゲー | フィブリノーゲン検査 |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20220146149A (ko) | 2021-04-23 | 2022-11-01 | 전남대학교산학협력단 | 한국형 살무사과 사독 감별용 조성물 |
KR20220170290A (ko) | 2021-06-22 | 2022-12-29 | 전남대학교산학협력단 | 한국형 살무사과 사독의 항사독 조성물 |
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