KR100338521B1 - Plant extract for eating activating macrophage - Google Patents

Abstract

The present invention relates to an edible plant extract having a macrophage activating ability, and more specifically, edible selected from the extract ( Curcuma zedoaria ), Chinese leek ( Chem : Allium tuberosum L. ) and fern (C : Pteridium aquilinum ) As a natural extract obtained by solvent extraction and alcohol extraction of plants, it is harmless to the human body. Especially, the macrophage activating ability is superior to the sugar fat which is widely used as a conventional macrophage activator. It relates to an edible plant extract having.

Description

Plant extract for eating activating macrophage

The present invention relates to an edible plant extract having a macrophage activating ability, and more specifically, edible selected from the extract ( Curcuma zedoaria ), Chinese leek ( Chem : Allium tuberosum L. ) and fern (C : Pteridium aquilinum ) As a natural extract obtained by solvent extraction and alcohol extraction of plants, it is harmless to the human body. Especially, the macrophage activating ability is superior to the sugar fat which is widely used as a conventional macrophage activator. It relates to an edible plant extract having.

There is no need to reiterate that combating and preventing cancer is one of the great challenges that life sciences face in the 21st century. Until now, as a method of treating cancer, there have been many methods of administering chemotherapy as well as surgical surgery and radiotherapy. However, the existing chemotherapeutic agents not only show toxicity and side effects for normal cells, but also have been proposed to cause cancer cells tolerate such chemotherapeutic agents. Therefore, studies have been continuously made to develop anticancer agents that selectively act only on cancer cells and have low toxicity.

With the development of immunology, various immune functional substances have been developed as immunotherapeutic agents for improving or treating the immune dysfunction state of a living body and are used for the treatment of cancer. The origin of these immunotherapeutics is various, including higher plants, such as bacteria, fungi, and the human body. However, some immunotherapeutic agents have severe side effects in vivo upon clinical administration.

In recent years, more advanced implementations and extensive analysis of the response of the biological immune system to cancer have led to several mechanisms that explain the host's defenses against tumors. Mechanisms that directly act on tumor cells, resulting in growth inhibition or death, mechanisms that enhance the immune response of a living body, mechanisms that act on a mediator that activates the defense system or increase host cancer's ability to defend against cancer or host immunity to tumor cells The mechanism of increasing the sensitivity to the system to increase the antitumor effect of the host has been proposed. In addition, mechanisms for promoting undifferentiated tumor cells into undivided mature cells, and increasing the effect of tumor treatment by reducing the damage to the cytotoxicity of living organisms following chemotherapy or radiation therapy for tumors have been published. . Substances that exhibit these mechanisms are called bioresponders, and unlike synthetic chemotherapeutic agents, they can affect biological responses of the body and thus have an inhibitory effect on cancer, thereby reducing side effects and increasing effects. You can.

Macrophage activator, an immune system enhancement material, is classified as a next generation food and drug new material in the treatment of various new diseases and incurable diseases as well as cancer among food and drug materials that have been researched and developed up to now. The immune system is largely divided into the innate immune system and the acquired immune system. The innate immune system is a macrophage phagocytosis, complementary pathway, and an immune response driven by the skin and epithelium.

The acquired immune system is an immune response to pathogens that have passed the innate immune system, which can be divided into humoral and cellular immune systems. Humoral immune systems are blood responses to antibodies produced in B lymphocytes. It is an immune response in tissue in vivo that occurs when a macrophage presents antigens to T cells and T cell helpers.

As such, macrophages are immune cells involved in both responses of the innate or acquired immune system and are known to have effector functions of antimicrobial, anticancer, and tissue damage.

Among the natural substances that exist in the natural world, various substances with various functions for living organisms are expected to increase in interest, and in particular, the substances with immune activity from food ingredients, herbal medicines and fungi are obtained. Efforts are ongoing. In particular, herbal medicines are known to have an important effect on the immune system, the endocrine system, the circulatory system, and certain herbal medicines are also used for autoimmune diseases such as chronic infection, rheumatoid arthritis, and nephrosis. The effect is clinically recognized. They are mainly active in polymer fractions rather than in low molecular fractions. Especially among these herbal-derived polymeric substances, interferon-inducing activity, fission promoting activity, anti-complementary activity, anti-tumor activity, anti-inflammatory activity, lymphocyte proliferation activity and Immunomodulatory activities such as phagocytosis enhancing activity have been found, and for some of these activities, it has been found that the active ingredient is polysaccharide.

Most of the plant-derived polysaccharides are macrophage-activating components reported from natural products. Especially, these polysaccharides exhibit both anti-cancer activity and anti-complementary activity, and also have antiviral activity, recticuloendothelial activation, and anti-inflammatory activity. It also has hypoglycemic activity. In fact, polysaccharides such as lentinan from shiitake mushrooms have been reported for their anticancer effects, antibody activity and macrophage activity. The main component is known as protein-linked polysaccharide or polysaccharide. Recently, antitumor effects of several plant-derived polysaccharides have been known, and their substrates are now understood as a result of the host's defense against tumors, i.e., the enhancement of the immune system, rather than direct cytotoxicity to cancer cells. . In particular, macrophages, which play an important role in the host's nonspecific defense system, are stimulated and activated by these polysaccharides, resulting in spreading, phagocytosis, antimicrobial activity through negative cell action, increased antitumor activity and direct damage to cancer cells. It is known to attack cancer cells by giving opsonin action and chemical stimulation movement following activation of polysaccharide complement system.

Therefore, by extracting a substance having a high degree of macrophage activation from the edible plant, there is a need for a substance that modulates immune function against a macrophage activator that is harmless to the human body and has superior activation than existing macrophage activators.

The present invention is to examine the degree of macrophage activation of various edible plants in order to prepare an extract for macrophage activator without side effects on the human body, obtained by solvent extraction and alcohol extraction from the edible plants, such as the extract under specific extraction conditions It is an object of the present invention to provide an extract and a method of extracting the superior macrophage activation ability through fractions.

In addition, an object of the present invention is to provide a purified powder having excellent macrophage activating ability separated from the extract and a method for separating purified powder.

Figure 1 shows the process of extracting the edible plant.

The present invention is characterized in that the edible plant extract having a macrophage activating ability, characterized in that the extraction of the solvent edible plant, and then extract the alcohol again.

In addition, the present invention in the preparation of the edible plant extract, extract the edible plant selected from the extract, Chinese leek and fern several times with an extraction solvent for 1 to 8 hours, and then extracted with alcohol again to obtain a precipitate fraction, macrophage activation It is characterized by a method for producing an edible plant extract having the ability.

In addition, the present invention comprises a method for producing a pure purified powder having a macrophage activation ability by fractionating the extract extract prepared according to the above method by ion exchange chromatography, hydrophobic interaction chromatography and gel filtration chromatography.

The edible plant extract, including the sequestration, having excellent macrophage activating ability according to the present invention is superior in macrophage activation than conventional macrophage activators, and is characterized in that it can be easily obtained from natural plants.

Extraction of edible plants such as Chinese leek, fern, etc., compared to conventional macrophage activators, by extracting traditional seasoned vegetables, western spices, and health vegetables, which were used for a long time Macrophage activation was excellent, in particular it was confirmed that the macrophage activation of the extract extract.

The extract used in the present invention has been widely used in traditional Korean medicine as a herbal medicine as a medicinal herb with turmeric and contains curcumin, which is a main ingredient of food flavoring agents in Southeast Asia, and its antioxidant and anti-inflammatory effects have been reported. Its use value is also high.

Chinese leek used in the present invention is a perennial herb of the family Liliaceae, and its habitat is Korea, China, Japan, etc. Among them, it is inhabited mainly in Korea and China, and is used as a branch or hemostatic agent for treating edible and medicinal, especially dysentery. The parts used as edible parts are stems and leaves, and they have been mainly eaten in the form of raw vegetables.

The fern used in the present invention is a perennial fern of the fern family, and the habitat is all over Korea, Japan, Okinawa, China, Sakhalin, Siberia, Europe, South and North America, and used as starch raw material, antipyretic, diuretic, diarrhea, jaundice treatment and medicinal medicine. have. The parts used as edible parts are young shoots, roots and underground stems.

According to the present invention, the extract is extracted several times with an extraction solvent for 1 to 8 hours to obtain a primary extract, which is then extracted with alcohol to prepare an extract having a macrophage activation ability, which is a precipitate fraction. Hereinafter, the manufacturing method will be described in detail.

At this time, the Chinese leek or fern in addition to the extraction can be extracted in the same or similar manner.

First, for example, by edible plant is represented by the boil at high temperature to remove the enzyme activity and pulverized with a homogeneous agent, so that the extraction is effectively made for a short time.

The pulverized sample is put into an extraction solvent and extracted with stirring for 1 to 8 hours to obtain a primary extract. If the extraction time is out of the above range, there is a problem that the macrophage activation is reduced. As the extraction solvent, it is preferable to use one or two or more mixed extraction solvents selected from acid, weak base, ethanol and distilled water, and more preferably distilled water.

Extraction and fern in the extraction process is preferably extracted at a temperature of 70 ~ 100 ℃ bar, when extracted at a temperature outside the above range, there is a problem that the macrophage activation is reduced.

In the extraction process, Chinese leek is preferably extracted at 4 ° C. to room temperature (25 ° C.), and when extracted at a temperature outside the above range, that is, at room temperature or higher, macrophage activation is rather reduced.

The primary extract of the edible plant thus prepared is lyophilized and the fraction is refluxed with methanol. The methanol extract is concentrated under reduced pressure, and the extract is concentrated under reduced pressure, and the extract aqueous solution is extracted with ethanol to separate the insoluble component, thereby obtaining a final extract of the edible plant selected from the extract, Chinese leek and fern.

Thus obtained edible plant extract based on the present invention according to the measurement of macrophage activation degree is superior to the conventional macrophage activator bar as a result, can be usefully used as a macrophage activator.

In addition, according to the present invention can be usefully used as an edible plant extract having a macrophage activation ability by adding to or in addition to the Chinese leek extract or fern extract in addition to the extract.

The edible plant extract of the present invention has the property of containing 30 to 60% by weight of sugar, 5 to 40% by weight of protein, 2 to 20% by weight of acidic sugar, and 5 to 35% by weight of other compositions.

In the present invention, the deactivation experiment of sugar and protein, respectively, it can be confirmed that the main body of macrophage activity is a sugar, and therefore the presence of the active body sugar rather than the ratio of the sugar and protein constituting the edible plant extract of the present invention Whether or not it plays a decisive role in macrophage activation.

In addition, the sugar contained in the edible plant extract of the present invention is 0.5 to 10 mol% of mannose, 3 to 30 mol% of galactose, 5 to 80 mol% of glucose, 1 to 20 mol% of rhamnose, 3 to 20 mol% of arabinose, and It contains 1-20 mol% of xylose.

The extract obtained through the extraction method according to the present invention is separated into a pure substance having excellent macrophage activation by performing ion exchange chromatography, hydrophobic interaction chromatography and gel filtration chromatography.

Macrophage activator containing an edible plant extract or purified powder based on the extract according to the present invention as an active ingredient can be consumed and used as food, but may be administered in a medicinal form, when used as a medicament, Or by parenteral or parenteral methods.

The dosage of macrophage activators is generally 0.1-400 mg / day based on adult patients having an average weight of 70 kg. Thus, in general adult patients, one dose or several administrations, once or several times a day, may be formulated into tablets or capsules containing pharmaceutically acceptable excipients or carriers to contain 0.05-200 mg of the effective extract. It is taken in dose. The above dosages are illustrative of the average case and may be higher or lower depending on individual differences.

In human application, the extract of the present invention may be administered alone, but may be administered in admixture with a pharmaceutical carrier selected in consideration of general mode of administration and standard pharmaceutical practice.

At this time, the carrier is glycerin, peanut oil, polyvinylpyrrolidone, olive oil, ethanol, benzyl alcohol, propylene glycol, water and other liquid carriers, lactose, kaolin, sucrose, crystalline cellulose, corn starch, talc, pectin, agar. Solid carriers such as stearic acid, magnesium stearate, lecithin, sodium chloride and the like can be used.

In addition, the macrophage activator of the present invention can be prepared in a variety of forms when used for food or medicament, for example, when using a liquid carrier, it is formulated with emulsion, syrup, soft gelatin capsules, gels, etc. When using a solid carrier, it can be prepared as tablets, capsules, powders, granules and the like.

Hereinafter, the present invention will be described in more detail with reference to Examples, but the present invention is not limited by Examples.

Examples 1 to 3: Preparation of edible plant extracts by lineage extraction

Three kinds of edible plants, such as bonsai, Chinese leek, and fern, were systematically extracted according to the method of FIG. 1.

First, edible plants are boiled at 100 ° C. for 5 minutes to remove enzymatic activity, and pulverized with a homogenizer for 20 minutes at 7,000 rpm. The ground sample was centrifuged at 5,000 rpm for 30 minutes, and the supernatant obtained here was lyophilized to prepare Fraction 1.

In the above process, the precipitate was continuously extracted with hexane for 2 hours after lyophilization, and then the extract was centrifuged to concentrate and lyophilize the supernatant, thereby obtaining fraction 2.

The precipitate obtained here was extracted for 2 hours in the order of acetone, methanol and distilled water in the same manner as described above and centrifuged to obtain fractions 3, 4 and 5 by concentrating and lyophilizing each supernatant.

Comparative Examples 1 to 22

As shown in Table 2, various edible plants purchased commercially were systematically extracted in the same manner as in Examples 1-3.

Experimental Example 1 Measurement of Macrophage Activation

Macrophage activation of the fractions 1, 4, and 5, which can measure the activity, was measured in fractions 1 to 5 of the edible plant extracts of lineage-extracted Examples 1 to 3 and Comparative Examples 1 to 22 by the same method.

First, each fraction prepared by phylogenetic extraction was dissolved in physiological saline to make an extract solution of 100 ㎍ / ml.

Hepes, Penicillin / Streptomycin, and amphotericin B within 48 to 72 hours after injecting 1 ml of thioglycollate medium into the abdominal cavity of 5-8 week old male ICR mice. Washed with RPMI-1640 medium containing (Amphotericin B) and macrophages were harvested intraperitoneally of mice. The recovered macrophages were washed twice with RPMI-1640 medium and redispersed in RPMI-1640 medium so that the cell number was 1 × 10 ⁶ cells / ml. This dispersion was dispensed into each well of a 96-well plate (180 μl) and incubated for 2 hours at 37 ℃, 5%, carbon dioxide. When macrophages form a monolayer in each well, non-adherent cells are washed twice with RPMI-1640 medium and removed, followed by 180 μl of RPMI-1640 medium containing 10% fetal bovine serum in each well. Dispense and add 20 μl of the extract solution prepared above, and incubate for 24 hours at 37 ° C., 5%, and carbon dioxide to activate macrophages. 0.1 mM Triton X-100 (25 μl) was added to the monolayer of activated macrophages to dissolve the cell membranes of macrophages. As a substrate of lysosome phosphatase, 10 mM, ρ Nitrophenyl phosphate (150 μl) was added together with 0.1 M citrate buffer (50 μl) to react in acidic condition for 1 hour, and the reaction was stopped by addition of 0.2 M borate buffer solution followed by ultraviolet spectrophotometer (ELISA reader, Bio). Absorbance at 405 nm with -Rad 3550-UV).

The absorbance of each extraction solution retrieved in this manner was compared with the absorbance of physiological saline, a negative control group, and this value was expressed as a macrophage activation diagram.

Table 1 shows the results of the first search for the degree of macrophage activation of Examples 1-3. In addition, the results of the first search for the macrophage activation level of Comparative Examples 1 to 22 are shown in Table 2.

As can be seen in Tables 1 and 2 above, the primary search results show that the hot water extract of the extract is the most excellent, and that the macrophage activation levels of the cold water extract of Chinese leek and the hot water extract of bracken are different. Higher than plants.

Therefore, the following experiment was carried out on the extract, Chinese leek, and fern.

Examples 4-15, Comparative Examples 23-34: Extraction conditions

In preparing each edible plant extract for extract, Chinese leek and fern, we tried to find the extraction conditions to maximize macrophage activation and yield. Each extract was prepared by different extraction solvent, extraction temperature or extraction time, and the macrophage activation and yield according to each condition were examined.

Distilled water, acid, methanol, acetone was used as the extraction solvent, and the concentration of the extraction solution was made to 100 ㎍ / ml. Extraction temperature was changed from 4 ℃ to 100 ℃, extraction time was changed from 2 hours to 24 hours, and the macrophage activation and yield according to the extraction conditions were measured.

As a result of the experiment, the macrophage activation and yield of the extract in Table 3, the macrophage activation and yield of Chinese leek in Table 4, the macrophage activation and yield of ferns are shown in Table 5.

As can be seen in Tables 3 to 5, the macrophage activity of the extract extracted from the extract, Chinese leek, and fern with different extraction solvents showed relatively low activity in organic solvents such as methanol and acetone. While the yield was low, the distilled water extract showed high activity and high yield.

In addition, hot water extracts were better in macrophage activation than cold extracts in the case of extracts and ferns, and cold water extracts were better in macrophage activation than hot extracts in Chinese leek.

On the other hand, when the extraction time was 3 hours, the macrophage activation and yield showed the maximum value, and after 10 hours, the macrophage activation capacity and yield showed a sharp decrease.

Example 16: Alcohol Extraction

500 g of ground milled extract was extracted with hot water at 100 ° C. for 3 hours. This process was repeated three times to extract the extracts were lyophilized. Since the fourth extract was rapidly reduced in yield and relative activity, the extract was prepared by collecting only 1-3 extracts.

Then, the extract was refluxed five times with methanol to separate methanol soluble components. The precipitate, a methanol insoluble component, was concentrated under reduced pressure, dissolved in an appropriate amount of distilled water, and extracted with ethanol to obtain an ethanol-soluble component and an ethanol-insoluble component.

As a result of measuring the macrophage activation effect of each fraction, methanol soluble fraction showed low activity of 130%, ethanol soluble fraction showed high activity of 225% and ethanol precipitation fraction showed the highest activity of 270%. Therefore, the following experiment on the ethanol precipitation fraction, the edible plant extract in the present invention refers to the ethanol precipitation fraction.

Chinese leek according to the extraction method of the above-mentioned extract, was extracted with distilled water at 25 ℃ to prepare a primary extract. Chinese leek extract showed a macrophage activity of 223%, slightly higher than that of the primary extract.

Also about the fern extract was obtained according to the extraction method of the above-mentioned. Fern extract showed 235% macrophage activation. The low molecular weight, fern methanol or ethanol soluble components, did not appear to be active, which seemed to coincide with the fact that most of the immunoactive substances reported so far were polysaccharides of polymer fractions rather than low molecular fractions.

Experimental Example 2 Composition of the Extract

The chemical composition of each edible plant extract, such as extract, Chinese leek and fern, was searched by spectrophotometer by color reaction.

Extracts for each edible plant, such as extract, Chinese leek, fern, and the respective chemical compositions are shown in Table 6.

Experimental Example 3 Composition of Sugar

The sugar of each extract used in Experimental Example 2 was analyzed per component using gas chromatography and thin layer chromatography.

The analysis results per component of each extract are shown in Table 7.

Experimental Example 4 Examination of the Body of Macrophage Activation

Since extracts, Chinese leek extracts, and fern extracts are mainly composed of sugars and proteins, the macrophage activity was examined by deactivating sugars or proteins, respectively, to identify macrophage activator substances.

Each edible plant extract was selectively inactivated by protein degradation by pronase treatment, and then examined for macrophage activation. In addition, the macrophage activation was examined by selectively inactivating the sugar site using a periodate.

The macrophage activity measurement results are shown in Table 8.

As can be seen in Table 8, the protein inactivation showed almost the same value or a slight decrease in macrophage activity, whereas the inactivation of sugar significantly reduced the macrophage activity. Therefore, the macrophage active body of each extract of the extract, Chinese leek, and fern, which is the material of this experiment, is polysaccharide and it is assumed that some protein is involved. In addition, it was found that the presence of an active body sugar rather than the composition ratio of sugar and protein has a decisive effect on macrophage activity.

Example 17 Separation and Purification of the Extract

The extracted extract, which had the highest macrophage activity among the fractions separated during mass extraction, was eluted according to the ionic strength of the sample components using an DEAE-Toyopearl 650C column (Cl-form, 3.5 × 38 cm), an anion exchange resin. DW elution fractions were CZ-1-0, CZ-1-I eluted at 0.1 M sodium chloride, CZ-1-II eluted in 0.2 M sodium chloride solution, CZ-1-III eluted at 0.3 M sodium chloride, 0.4 CZ-1-IV eluted at M sodium chloride, CZ-1-V eluted at 0.5 M sodium chloride, CZ-1-VI eluted at 0.6 M sodium chloride, CZ-1-Ⅶ eluted at 0.7 M sodium chloride The activity of each macrophage was measured by separating into 8 fractions of.

According to the method, the macrophage activation and yield measured for the eight fractions separated are shown in Table 9.

The chemical composition of each fraction showed the highest sugar content of CZ-1-0, which is the DW eluting fraction (61% per sugar), but the macrophage activation was very low, whereas the macrophage activation was high in CZ-1-III and CZ-1. -IV fractions showed 14.7%, 17%, 30%, and 40.8% of sugar, respectively, and as the sodium chloride concentration increased, the sugar content decreased and the protein content increased.

Example 18 Fraction of CZ-1-IV

CZ-1-IV, a 0.4 M sodium chloride nonadsorbed fraction on DEAE-Toyopearl 650C, was subjected to hydrophobic interaction chromatography according to the sodium chloride reverse concentration gradient by butyl-Toyopearl 650M. Separated into eluted fractions. These fractions showed similar activity, respectively, indicating that CZ-1-IV-A and CZ-1-IV-B exhibited high activity of 220% or higher at 1.0 mg / mL, but showed high activity of CZ-1-IV-A. It refined as a main active ingredient. In chemical composition, CZ-1-IV-A contains 71.7% total sugar, 16.7% protein, and CZ-1-IV-B shows 9.8% total sugar and 88.6% protein. It became.

Gel filtration chromatography was performed by selecting a Sepharose CL-6B column (2 × 90 cm) capable of fractionating CZ-1-IV-A, which had high activity and polysaccharide content in the fraction eluted from butyl-Toyopearl 650M. The result was separated into two fractions, CZ-1-IV-A1 and CZ-1-IV-A2. CZ-1-IV-A1, a substance having a molecular weight that can be fractionated with Sepharose CL-6B, was eluted in the elution volume and showed high activity of 250% or more at 100 ㎍ / mL. CZ-1-IV-A2 showed low macrophage activity.

CZ-1-IV-B was also subjected to gel filtration chromatography using a Sepharose CL-6B column (2 × 90 cm), resulting in CZ-1-IV-B1, CZ-1-IV-B2, and CZ-1. Three fractions of -IV-B3 were isolated, of which CZ-1-IV-B2 fractions exhibited relatively high activity of 201% at a concentration of 100 µg / mL.

Therefore, two fractions CZ-1-IV-A1 and CZ-1-IV-B2 having high macrophage active ingredients and different chemical compositions were obtained during the isolation and purification of macrophage active material.

Experimental Example 5: Short-term toxicity experiment in vivo

The acute toxicity of the extract was investigated. By oral administration, the extract extracts were not toxic in all test groups with a sample volume of 0 to 2,000 mg / kg and it was estimated that LD ₅₀ was present at 2,000 mg / kg or more.

In the toxicity test by parenteral administration, the sample was injected intravenously into the tail of the mouse with a sample volume of 0 ~ 1,000 mg / kg, and the toxicity was observed for 1 week. It was confirmed that no toxicity was shown.

The results of toxicity experiments by oral administration are shown in Table 10, and the results of toxicity experiments by parenteral administration are shown in Table 11.

Example 19 Preparation of Tablets

2 mg extract extract

Lactose 14 mg

3 mg of crystalline cellulose

Magnesium Stearate 1 mg

The ingredients listed above were finely mixed and then tablets were prepared by a direct tableting method. The total amount of each tablet was 20 mg per tablet, of which the extract content was 2 mg.

As described above, in the present invention, the edible plant was first extracted to search for edible plants having excellent macrophage activation ability. As a result, the extracted extract had the best macrophage activation ability, and the Chinese leek and fern extract had the excellent macrophage activation ability.

Extracts of Chinese leek and fern, respectively, were extracted with solvent and alcohol, and each edible plant extract was obtained in large quantities. The edible plant extracts were found to be very useful as macrophage activators because of their excellent macrophage activation ability. In particular, pure purified powder having excellent macrophage activation ability was isolated from the extract.

As described above, the edible plant selected from the extract, Chinese leek and fern according to the present invention, particularly the extract obtained by extracting the macrophage activation ability is significantly superior to the conventional macrophage activator, especially harmless to the human body, natural plants Since it can be easily separated from the macrophage activator has a very useful effect.

Claims (13)

After extracting the edible plant extract with distilled water, it is extracted again with a macrophage activation ability, characterized in that the extraction with alcohol of methanol or ethanol again. The extract of claim 1, wherein the Chinese leek extract or fern extract is added to the extract extract alone or in combination. According to claim 1 or 2, wherein the edible plant extract is characterized in that it contains 30 to 60% by weight sugar, 5 to 40% by weight protein, 2 to 20% by weight acidic sugar, 5 to 35% by weight of other compositions. Extracts having a macrophage activating ability to. The sugar composition according to claim 3, wherein the sugar is 0.5 to 10 mol% of mannose, 3 to 30 mol% of galactose, 5 to 80 mol% of glucose, 1 to 20 mol% of rhamnose, 3 to 20 mol% of arabinose and 1 to Xylose. Extract containing the macrophage activation ability, characterized in that it contains 20 mol%. In preparing edible plant extracts, macrophages characterized in that the edible plant selected from the extract, Chinese leek and fern is extracted several times with distilled water for 1 to 8 hours, and then extracted with alcohol of methanol or ethanol to obtain a precipitate fraction. Method of preparing an edible plant extract having an activating ability. delete 6. The method of claim 5, wherein the extract and fern have an extraction temperature of 70 to 100 ° C. [6] The method of claim 5, wherein the Chinese leek has an extraction temperature of 4 ° C. to room temperature (25 ° C.). 6. The method of claim 5, wherein the alcohol extraction is refluxed with methanol, and then concentrated under reduced pressure, and further extract the concentrated solution solution under reduced pressure with ethanol to obtain a macromolecular activation ability, characterized in that Method for producing an edible plant extract. delete delete The method of claim 5, wherein the precipitate is fractionated by ion exchange chromatography, hydrophobic interaction chromatography, and gel filtration chromatography. An immunopotentiator, characterized in that the extract obtained by the method of any one of claims 5 to 12 contained as an active ingredient to activate macrophages.