KR100330963B1 - Disfection method and device using herb oil to disinfect relic and record - Google Patents

Abstract

end. The technical field to which the invention described in the claims belongs.

The present invention relates to the field of the control of records for sterilizing, disinfecting and permanently preserving the records of valuable national treasures, cultural artifacts, ancient books, old documents, etc., in particular harmless to the human body with excellent fungi and aroma effects ( herb) The present invention relates to a method of disinfecting relics and records and an apparatus for sterilization, which are easy to handle and safe by using essential oils.

I. Technical problem of the present invention.

At present, there are gas fumigation methods using MB (Methyl bromide) / EO (Ethylene oxide) mixed gas with strong bactericidal power, but MB / EO mixed gas itself As it is very toxic and can cause fatal damage to human body even at low concentrations, there is a high risk of handling and use.In addition, due to this risk, a separate treatment device is required at the rear of the disinfection device to purify the gas used for disinfection. Therefore, there is a problem that the disinfection system is complicated.

All. Summary of the Solution of the Invention.

It is harmless to human body and uses antibacterial agent made of herbal essential oil that has stronger bactericidal power than previous MB / EO mixed gas, and it uses nitrogen gas inside vacuum sterilizer which is maintained at saturated steam pressure. By spraying with ultra-fine particles to facilitate the penetration of antimicrobials into artifacts and recordings, by the fungicidal action in the anaerobic state it is possible to have a clear control effect by natural herbs.

la. Important use of the invention.

Disinfection of Artefacts and Records.

Description

Disinfection method and device using herb oil to disinfect relic and record for disinfection of artifacts and records

The present invention relates to the field of the control of artifacts and records for sterilization, disinfection and permanent storage of valuable national treasures, ancient books, ancient documents, etc., especially harmless to humans and excellent fungi and aroma effects It relates to a method and apparatus for disinfection using essential oils of herbs.

In general, relics and records are damaged by the various airborne bacteria (fungus, bacteria) inhabiting the library where the relics and records are stored. Most relics and archives, including government archives, which manage ancient documents, etc., are designed to permanently preserve relics and records by sterilizing and disinfecting various microorganisms that cause damage to relics and records.

To this end, as a means of storing the recording media in the warehouse, the gas fumigation method using MB (Methyl bromide) / EO (Ethylene oxide) mixed gas is being disinfected. This material is very toxic by itself. It is strong and has excellent bactericidal power, so it shows excellent effect as a countermeasure against relics and records.However, if leaked during work, it causes fatal damage to human body even at very small concentrations. There is a high-risk problem, and even after disinfection is completed, there is a problem that strong toxic components remain in artifacts and records for a long time to damage the human body.

In addition, the vacuum fume, which is a disinfection device implemented by the above method, is more difficult to manage and handle due to the high possibility of damage to the pipe due to the toxic gas and the high risk of leakage of the toxic gas through the damaged pipe and the aged pipe. 6, a heating apparatus 300 for fumigation of the mixed gas 200 and a separate processing apparatus for purifying the gas discharged to the outside after disinfection must be installed at the rear end of the sterilizer 100. As a result, the disinfection system is complicated, and the MB gas in the mixed gas component can be completely removed even with the activated carbon adsorption device 400 and the water dissolving device 500, which have been used as a treatment device for purifying gas. There is no problem.

In addition, there is a problem that even the vacuum fume can not be widely used in other areas that require disinfection because there is a usage restriction that can be used only by those who are legally licensed in that it uses toxic gas.

In addition, the use of these substances is increasingly regulated internationally due to the air pollution caused by the destruction of the ozone layer.

Therefore, the present invention has been made in order to solve the above-mentioned conventional problems, maintaining an antibacterial agent made of essential oils having excellent fungicidal power among vegetable essential oils (herb) harmless to the human body in a saturated steam pressure state. It is sprayed into ultra-fine particles by using compressed nitrogen gas into the vacuum sterilizer, which is a vacuum, so that antimicrobial can penetrate into relics and recordings. It is to provide a method and apparatus for disinfecting relics and records for complete processing.

1 is a flow chart of the disinfection system of relics and recordings according to the present invention.

2 is a further disinfection system flow chart of artifacts and recordings in accordance with the present invention.

Figure 3 is a schematic diagram showing the configuration of the disinfecting apparatus according to an embodiment of the present invention.

Figure 4 is a schematic diagram of the configuration of the disinfection device to be injected into the antimicrobial agent by the ultrasonic spray device.

5 is a structural diagram showing the principle of the spray apparatus applied to the disinfecting apparatus of FIG.

6 is a flow chart for disinfecting artifacts and recordings by a conventional method.

Explanation of symbols on the main parts of the drawing

1: Sterilizer 2: Antimicrobial Container

3: vacuum pump 4: gas cylinder

5: control panel

Hereinafter will be described in detail with reference to the accompanying drawings.

First of all, in the present invention, herbal refining was used as a component that is harmless to the human body and has a strong bactericidal power for disinfection.

Currently, the herb refers to the leaves of herbs, herbs, etc., or is a generic term for fragrant grasses native to the Mediterranean coast or Southwest Asia, and recently includes the leaves, flowers, fruits, etc. It refers to all plants that have.

Herbal oil is extracted from these plants, and the essential oils of these herbs have fungicidal effect at the same time as aromatic and can be used as an antimicrobial agent. Since these antimicrobials are extracted from natural plants, they are harmless to the human body. .

Therefore, in the present invention, various methods for finding herbal refining oils having the same or superior antibacterial effect as MB (Methyl bromide) / EO (Ethylene oxide) mixed gas, a chemical disinfectant previously used as a gas for disinfection. As a result of the experiment, it was confirmed that savory (savory) has the best bactericidal effect among the herb essential oils.

To obtain the experimental results, 10 fungi and 5 bacteria were prepared.

The growth medium of the test strain was LB agar (tryptone 10g, Nacl 10g, yeast extract 5g / ℓ) for bacteria, PDA (Potatoes, infusion from 200g, Dexrose 20g, agar 15g / ℓin 10% tartaric acid ( 70% Sigma, USA) was added at a rate of 1.9 mL / 100 mL).

As a measure for antimicrobial activity, the bacterial and fungal cultures in which the whole culture for 2 days and the main culture for 4 days were completed were centrifuged at 3000 rpm for 15 minutes to separate the supernatant and the bacteria, and the in vitro antimicrobial activity assay was performed on bacteria and fungi. Was carried out.

LB agar was used as a bacterium, potato dextrose agar was used as a fungus, and a black plate was prepared by using a middle black plate and a single layer black plate according to the type of bacteria.

The preparation of the middle-layer black plate was made by pouring 20 ml of the medium into a petri dish to coagulate to make a lower layer, and inoculated with the shaken or stationary culture using the same strain into 4 to 5 ml of the medium on the lower layer.

The monolayer black plate was prepared by using about 5 ml of the medium inoculated with the bacteria as a single layer.

Measurement of antibacterial activity after incubation of each test organism 18 ~ 24 hours (fungi 7 days using the prepared spore solution from a culture slant) to be 10 ⁶ ~ 10 ^{7 cell} / ㎖ plated on petri dish and then paper disc (ø6mm) Various samples were added to the flask, and incubated at 25 ° C. (mold) and 37 ° C. (bacteria) for 24 to 48 hours.

Antimicrobial activity is to measure the presence and absence of a clear zone (clear zone), shown in Table 1 below.

Table 1

microbe Natural Antibacterial Remarks Alternaria alternata 20 mm mold Aspergillus niger 26 mm Aspergillus oryzae 18 mm Actinnobacillus ureae 20 mm Mucor mucedo 18 mm Mucor rouxii 16 mm Neurospora sitophile 18 mm Penicillium notatum 24 mm Rhizopus dlimer 22 mm Thamnidium elegans 18 mm Bacillus cereus 20 mm Germ Bacillus megaterium 18 mm Pseudomonas aeruginosa 26 mm Staphylococcus aureus 24 mm Staphylococcus auricularis 20 m1m

In addition, in order to confirm the bactericidal effect of the natural antibacterial and chemical disinfectant (MB / EO), bacteria and fungi were incubated for 3 days, and the culture solution was added to 50 μl (10 ⁵ cfu) on a paper disk. Into and tested for antimicrobial activity.

After disinfection, paper disk was diluted in steps to measure the number of microorganisms and the effect is shown in [Table 2] below.

[Table 2] Killer fungi of natural antibacterial and chemical antiseptics (M.B / E.O)

microbe Microbial count (cfu) Remarks Chemical disinfectant Natural Antibacterial Alternaria alternata 10 ³ 10 ¹ mold Aspergillus niger 10 ⁴ 10 ¹ Aspergillus oryzae 10 ² 0 Actinnobacillus ureae 10 ¹ 0 Mucor mucedo 10 ³ 0 Mucor rouxii 10 ³ 0 Neurospora sitophile 10 ² 0 Penicillium notatum 10 ⁴ One Rhizopus dlimer 10 ³ 0 Thamnidium elegans 10 ³ One Bacillus cereus 10 ³ 0 Germ Bacillus megaterium 10 ² 0 Pseudomonas aeruginosa 10 ³ 0 Staphylococcus aureus 10 ⁴ 0 Staphylococcus auricularis 10 ⁴ 0

Therefore, as a result of the above experiment, it was proved that herbal refining, ie, natural antimicrobial agent showed higher antimicrobial activity than MB / EO mixed gas for the number of microorganisms remaining and the multiplication state of microorganisms.

Meanwhile, as an alternative to the herbal extracts used in the present invention, dill (Aillhum graveolens), lavender (Lavender; Lavandul spica), lemon balm (Melissa officinalis), rosemary (Rosemary; Rosmrinus officinalis), mint (Mint) ; Mentha spp., Savory (satureia hortensis), Sage (Salvia officinalis), Scentedgeranium (Pelargonium spp.), Self hill (Prunella vulfaris), Sweet basil (Ocimum) basilicum, oregano (origanum vulgare), tanji (Tansy; Tanacetum vulgre), cinnamon (cinnamonum ceylanicum), clove (Clove; Eugenia caryophyllata), eucalyptus (Eucalyptus; Euucalytus spemon. limonum), Manuka (Leptospermium scoparium), Peppermint (Mentha piperita) and Tea tree (Melaleuca alternifolia).

As an example of the disinfection method using the essential oil as described above, as shown in Figure 1, the antibacterial agent made of the essential oil (精油) of the herb (herb) compressed nitrogen gas into the sterilizer in a vacuum state is maintained at a saturated steam pressure state By spraying with ultra-fine particles of less than about 10㎛ size using the antimicrobial agent to penetrate the artifacts and recordings, and by the injection of vacuum and nitrogen gas in order to achieve an antibacterial in the anoxic state.

In this case, the degree of vacuum inside the sterilizer is maintained at -550 to -650 mmHg so that the antimicrobial agent penetrates the artifacts or records well. If the vacuum degree is less than -550 mmHg, the antimicrobial agent penetrates the artifacts or records. Even in the above case, the penetration of the antimicrobial agent is almost the same as in the case of the vacuum degree of -550 to -650 mmHg.

In addition, the nitrogen gas used for the compression spraying of the antimicrobial agent is compressed to a pressure of 3Kg or more, and the particles of the sprayed antimicrobial agent have a size of 2 to 3 µm, which is a size at which no liquefaction occurs at the surface when the spraying liquid is close to a hand or an object. Is most desirable. It was confirmed that no damage occurred to the relics or the recorded materials even when the size was 10 μm or less.

In addition, as shown in FIG. 2, after spraying the antimicrobial agent into the sterilizer, the compressed nitrogen gas is momentarily injected above atmospheric pressure, and the injected nitrogen gas pushes out the oxygen gas remaining deep between the artifact and the recording material. Even a small amount of oxygen gas in contact with the can be completely blocked to obtain a more effective killing effect (bacteria) effect.

Nitrogen gas, which is injected into the sterilizer, is harmless to the human body and blocks oxygen necessary for the growth of microorganisms with the microorganisms without affecting the materials of the relics and recording materials, including argon (Ar). Injecting any gas that can produce an effect will achieve the object of the present invention.

Therefore, since the microparticle-type antimicrobial agent is sprayed into a vacuum sterilizer in which a natural antimicrobial agent using a herb is maintained in a saturated steam pressure state, particulate antimicrobial particles penetrate deeply between the relics and the recording material, thereby causing a bactericidal reaction. In particular, by using nitrogen gas instead of air as a compressed gas for adding an antimicrobial agent, the maximum disinfection effect can be obtained by completely blocking the oxygen necessary for the growth of microorganisms.

Indeed, this effect was demonstrated in the case of a mixed breed of pest species common in museums, where a mortality of 100% was observed when leak-sealed in nitrogen gas for 1, 2 and 3 weeks, respectively. It is sufficient to demonstrate that the pesticidal effect of nitrogen on pests.

In this case, nitrogen (N) on the sterilizer space (㎥) for 100% mortality₂) The gas injection amount is estimated to be about 50 mmg.

Next will be described in detail by the embodiment of the configuration of the disinfection apparatus for performing the disinfection method according to the present invention.

As shown in FIG. 3, the disinfection apparatus of the present invention is a sterilizer (1), the antimicrobial agent is put into the container (2) to secure a space for disinfection by putting the antibacterial agent to put the remains and records to be disinfected In order to maintain the inside of the sterilizer (1) in a vacuum state, the vacuum pump (3) for smooth infiltration of the antimicrobial agent, and the compressed gas for spraying the antimicrobial agent contained in the antimicrobial agent container (2) into the sterilizer (1) It consists of a gas cylinder (4) and a control panel (5) for controlling this process in one place after disinfection.

The sterilizer 1 has a nozzle 11 for atomizing the antimicrobial agent introduced into the sterilizer to evenly spread and spray the entire interior of the sterilizer 1, and discharge the gas inside the sterilizer 1 after disinfection to the outside. A discharge port 12 for the purpose is installed, and a gauge 13 for measuring the degree of vacuum inside the sterilizer is installed.

At this time, since the degree of vacuum inside the sterilizer should be maintained at about -550 to -650 mmHg, it is advantageous to make the sterilization chamber into a circular cylinder to withstand high vacuum pressure.

In addition, the cartridge 14 for stacking the recording document or book in order to put it in the sterilizer is detachably installed so that it can be used appropriately according to the object of the disinfectant.

In addition, one side of the inside of the sterilizer is provided with a spray space (16) for spraying the antimicrobial agent by the nozzle (11) of the installation wall of the blocking wall (15) in which the vent hole (15a) is formed in the fog state with the nitrogen gas barrier wall ( Through the vent hole 15a of 15), it is moved to the space where the relic or the recording material is placed.

This prevents the liquid antibacterial sprayed from the nozzle 11 from directly contacting the relic or the recording material (especially the recording document). When the spray is properly sprayed by tilting the vent wall 15b, the antimicrobial agent in the fog form the relic or recording material. To move smoothly to the side, and the antimicrobial agent remaining in the spray space 16 is to be transported to the antimicrobial drain container 17 can be reused.

The pressure of the gas cylinder 4 must be maintained at all times so that the pressure of the compressed gas used for the compression spraying of the nozzle is always higher than or equal to 3 kg, which is an optimum spray pressure condition. The transfer between the gas cylinder 4 and the nozzle 11 is performed. The regulator 19 is provided in the pipe 18, and the output pressure is adjusted to be constant.

The antimicrobial input container (2) is engraved on the scale so that the amount of the antimicrobial agent injected and the amount consumed in the spraying can be known. Or determine the original sterilizer vacuum pressure.

The control panel (6) is to enable the disinfection process by a switch operation, and also to ensure and control the vacuum pressure of the sterilizer, the pressure of the compressed gas, the treatment time, etc. to ensure safe and effective disinfection.

In addition, this device measures the vacuum pressure in the sterilizer, the pressure of the compressed gas, etc. for safety and is installed with a safety device to shut off all the valves when the safety level is over, the compressed gas filled in the gas cylinder (4) Transfer pipe (18a) for separately inputting to the disinfection room is connected.

Therefore, the above disinfection apparatus is sprayed in the form of ultra fine particles by the spray device into the sterilizer in which the antimicrobial agent is maintained in a saturated steam pressure state, so the fine particles of the antimicrobial agent penetrate deeply between the relics and the recording material, thereby maximizing sterilization. You will get the effect.

On the other hand, Figure 4 schematically shows the configuration of the disinfection apparatus according to another embodiment of the present invention, the antimicrobial agent to the inside of the sterilizer (1a) by the spraying device 6 for spraying the antimicrobial agent by using ultrasonic vibrations It was sprayed into ultra-fine particles in a mist state, so that disinfection was performed.

The spraying device (6) is an antimicrobial reservoir (61), a water tank (63) formed with a piezoelectric element (62) for ultrasonic radiation on the surface layer, and a blower (64) for injecting the antimicrobial agent into the sterilizer (1). When the central sound pressure is concentrated and directed by the powerful ultrasonic wave generated in the piezoelectric element 62 formed in the tank 63, this power lifts the surface of the antimicrobial agent in the tank 63 to generate a water column. In addition, surface waves are generated on the surface of the liquid, and interference waves are generated on the surface around the water column, and the collision and tearing power of the liquid exceed the surface tension of the liquid and eventually out of the water to become fine particles. It uses the principle of being released into the air.

In addition, the disinfection apparatus may include a gas cylinder 4a filled with a compressed gas in order to inject a separate compressed gas after the injection of the antimicrobial agent into the sterilizer (1a).

Therefore, since the antimicrobial agent sprayed into the sterilizer is discharged as the fine particles in the vapor state from the spraying device 6, it is possible to disinfect the microscopic stains or spots due to the antimicrobial agent so as not to adhere to the remains and records.

Therefore, the method of disinfecting relics and recordings by the natural antimicrobial agent using the herb of the present invention is easy to handle and very safe disinfection method because the gas component used to inject the antimicrobial agent and the antimicrobial agent used for disinfection is harmless to the human body. Therefore, the disinfection device configured to be disinfected by this disinfection method does not need to install a separate treatment device for purifying the gas discharged to the outside after disinfection.

Therefore, since the disinfection method and the disinfection device of the present invention do not have any special conditions or handling problems, it can be variously used in all control fields including industrial and various laboratories, libraries, and laboratories that require antibacterial and fungicides. will be.

Claims (10)

The herb-based antibacterial agent is sprayed into ultra-fine particles using compressed nitrogen gas into a vacuum sterilizer maintained at a saturated steam pressure, so that the microorganism is completely blocked from oxygen. Disinfection of relics and recordings using a herb characterized in that the bacteria are made. The method of claim 1, comprising injecting compressed nitrogen gas above atmospheric pressure after spraying the antimicrobial agent. Disinfectant (1), antimicrobial input container (2), vacuum pump (3) to maintain the interior of the disinfectant (1), the space for the disinfectant is added and the antimicrobial agent is put into the disinfection process, the antibacterial agent Using a hub including a gas cylinder (4) containing a compressed gas for spraying the antimicrobial agent contained in the input container (2) through the nozzle (11), and a control panel (5) for controlling this process in one place after disinfection Disinfection of artifacts and records. The method of claim 3, wherein the disinfection room of the sterilizer (1) inside the disinfection apparatus of the relics and recordings using a hub, characterized in that to be manufactured in a circular cylinder to withstand the vacuum pressure. 4. The apparatus of claim 3, wherein the cartridge (14) for stacking recording documents or books into the sterilizer (1) is detachably installed. The hub of claim 3, wherein a spray space 16 for spraying the antimicrobial agent by the nozzle 11 is installed at one side of the inside of the sterilizer 1 by the installation of a barrier wall 15 having a vent hole 15a. Disinfection equipment of used artifacts and records. 7. The apparatus of claim 6, wherein the vent hole (15a) is inclined by an inclined vent wall (15b). 7. The apparatus of claim 6, wherein the antimicrobial agent remaining in the spray space (16) is transferred to an antimicrobial drain container (17). The antimicrobial agent made of essential oil of herb is applied to the spray container 6 using ultrasonic vibration formed by the antimicrobial container 61, the water tank 63 in which the piezoelectric element 62 is formed on the surface layer, and the blower 64. Disinfection device for relics and recordings using a hub sprayed into the sterilizer (1) in a vacuum maintained at a saturated steam pressure. 10. The apparatus for disinfecting relics and recordings according to claim 9, wherein the refining oil is extracted from a hub including a gas cylinder (4a) for injecting a separate compressed gas into the sterilizer (1).