KR100330804B1 - New plant variety of clover WonKyung and breeding method thereof - Google Patents

Abstract

The present invention relates to a new kind of clover 'far' and its breeding method, and selected from four wild leaf white clover mutant leaves and stems mutated to four leaves, and then repeatedly inserted into it to grow asexually, When completed, four heart-shaped leaves with a white oval band in the middle of the leaf gather to form a round circle, which is highly coronary in value, large in leaves and vigorous in growth, and excellent for feed, resistant to strong rays and resistant to disease, It is resistant to cold resistance and competition with weeds, and has a high speed of growth, and has an excellent effect of providing a new breed of 'clover' and a method of breeding it, which are very excellent for grassland composition and land improvement.

Description

New plant variety of clover WonKyung and breeding method

The present invention relates to a new breed of 'clover' and its breeding method. More specifically, the present invention is to insert a four-leaf mutant leaves, stems and pulposus by a three-leaf wild type white clover plant by the cultivation method and asexual propagation and harvesting the deleted seeds It is about a new four-leaf clover 'far', which has strong acquired properties, has abundant growth, and has four leaves, and has a large coronary value.

Popular clover includes white clover ( Trifolium repense L.) and red clover (Red clover, Trifolium pratense L.), depending on the color of the flower. Roots do not extend deep, straight roots, very low stem, three-year-old to perennial perennial herb. It is generally called white clover because it can be produced in the laparoscopic area, grows as an independent individual at the root node, and the color of flowers is especially white. The existing three-leaf clover is ovate in shape and generally has a V-shaped white or black pattern in the middle of the leaf, which is not beautiful in shape and is also preferred as a symbol of luck for legend in both East and West. The disadvantage is that it can only be obtained rarely by mutation. Since four-leaf clover means 'good luck', many people want to carry it, so it has a rare value and an excellent ornamental value, so it is an excellent crop that can be applied to gifts, dry flowers, and pressed flowers. There is no four-leaf clover that adapts well, is strong in nature, has strong growth, and has excellent merchandise and ornamental value. The naturalized clover which has been naturalized in Korea and has been adapted to Korea's climate and climate for a long time, has fast ground cover effect, leaves are green, leaves are round oval, and have a v-shaped white or black band in the middle. Since the stem is small, the leaves are not beautiful, and the four leaves are formed by mutations, it is difficult to find a large amount of commercial value.

Specifically, currently planted in Korea for feed is introduced from foreign countries, three-leaf clover, Kenland, Titus, Atlas, Thial 2000, etc. of the red clover family . However, these imported varieties are difficult to adapt to the climate and climate of our country, and many of them are difficult to grow. Therefore, the four-leaf clover of traits that are well adapted and robust to the climate and climate of Korea, has strong growth, and has excellent merchandise and ornamental value are required.

In view of the above facts, the present inventors have mutated from three-leaf wild species white clover and selected four-leaf mutant leaves and stems. A new breed of four-leaf clover with four leaves of heart-shaped shape with a band of was bred. The four-leaf clover has a high value in terms of its ornamental value because of its white oval bands in the middle of the leaf, forming a rounded circle, and it can be developed into various kinds of gifts by drying the leaves. . In addition, its large leaves and vigorous growth make it possible to produce high yields for feed, cultivation under strong light, strong disease resistance, strong competition with weeds, and fast growing speed, making short pasture grassland for use as pasture. Can also be used as.

Accordingly, it is an object of the present invention to provide a new kind of clover produced as a result of asexual reproduction by repeatedly cutting the mutant leaf and stem having four leaves selected from the wild species white clover.

Another object of the present invention is to provide a method for breeding the new breed of clover.

The above object of the present invention is to mutant from three-leaf wild species white clover (see Fig. 1) to cut and sterilize the laparoscopic including four-leaf mutant leaf and its stem, and then to activate cell division BAP, IBA And planting the plants treated with NH ₄ NO ₃ in pots, selecting the stems and inserting the stems to asexual breeding for five generations to obtain a new breed of clover of the present invention 'clover WonKyung' and the characteristics of the wild clover This was achieved by dispensing and inserting stems or roots of the 'far' after comparison to confirm the uniformity of the new species.

Hereinafter, the configuration of the present invention will be described in detail.

1 is a photograph showing a lap diameter including leaves, flowers, and stems of a conventional wild species white clover.

Figure 2 is a photograph showing the shape of the present invention clover new breed 'far' in the pot.

Figure 3 is a photograph of the clover new species 'far' leaf of the present invention.

Figure 4 is a photograph showing the phenotype of the leaves by cutting the creep diameter, root node and stem portion of the new clover new species 'far'.

Figure 5 is a photograph of a flower showing the state of the clover composed of three of the invention clover new breed 'far'.

According to the present invention, a mutant is obtained from a three-leaf wild species white clover mutated by four leaf leaves and stems, and then cut and cut by primary sterilization with ethanol, secondly with distilled water and bleach, followed by washing with distilled water. ; Treating BAP, IBA and NH ₄ NO ₃ to intensify cell division of the white clover fragment; Selecting a stem of a four-leaf clover obtained by planting the slices in a flowerpot and cutting the leaves over five generations to obtain a new breed of clover of the present invention 'clover WonKyung'; Investigating the characteristics of the new species of clover 'far' of the present invention and comparing with the wild species clover; Dispensing and inserting the stem or root of the clover new breed 'far' of the present invention consists of checking the uniformity of the new breed.

Hereinafter, specific examples of the present invention will be described in detail with reference to Examples, but the scope of the present invention is not limited thereto.

Example 1 Breeding and Characterization of the New Clover New Species of the Invention

First step. Breeding of new clover 'far'

In May 1994, 100 leaves and crawled stems of four leaves were extracted from the white clover as shown in FIG.

The collected stem was cut into 3 ~ 5cm size, soaked in 70% ethanol for 3 seconds and sterilized first, washed with sterilized distilled water, and then again used commercially available bleach (Yuhan Lax, Co., Ltd.) 10% (v / v) was immersed in 20-30 minutes for secondary disinfection and then washed with sterilized distilled water.

In order to enhance cell division of the sterilized white clover plants, BAP (Benzyl-amino-purine) 5.0 mg / L and IBA (3) were treated for 5 minutes at 40 to 45 ° C. for 10 seconds in cold water for 10 seconds. -indolebutyric acid) 0.5 mg / L and NH ₄ NO ₃ 1650 mg / L. When the shoots and roots of this plant are formed to some extent in the container, the soil is mixed with 1: 1: 1 peat moss, perlite, and sterilized sand in a greenhouse that has been constructed at a humidity of 90%, a temperature of 18 to 20 ° C, and a brightness of 60%. After transplanting in minutes, the water was watered at a temperature of about 15 ° C. at 8-9 am and 4-5 pm daily. As a result of the growth, more than 90% of the growth was normal. The stems of the four-leaf clover secured in this way were selected and repeatedly cut for five generations to grow asexually. In this process, four leaf clover began to appear in each individual, and as the process was repeated over and over again, the variation of the four leaf clover, such as leaf shape, leaf color, leaf pattern, growth characteristics (temperature, light, humidity, etc.), By continuously selecting individuals with excellent traits such as coating speed and breeding method, selecting and breeding (uniformity) of those having excellent nutritional status, ornamental value, environmental adaptability, disease resistance, commercial value, etc. A new kind of clover 'far' was obtained.

Second step. Growth Characteristics of New Clover New Species of the Invention

Investigate the characteristics of the new invention 'clover' of the above-mentioned clover, which was grown by inserting and deleting seeds which were deleted by the nutrient breeding method to make four-leaf clover from three-leaf wild species white clover and wild leaf consisting of three leaves (wild type) ) Compared to clover (differentiation). The results are shown in Table 1.

Comparison of characteristics of new clover 'far' and wild clover characteristic New breed 'far' Wild species key 10 to 20 cm 5-15 cm Number of leaves Four Three Leaf shape Heart-shaped, the tip of the leaf is V-shaped Oval Surface of the leaf Matte and downy No gloss The periphery of the leaf Comb pattern Comb pattern Leaf length 0.5-2.5 cm 1.0-2.5 cm Width of leaf 0.5-2.5 cm 0.5-2.0 cm Color of leaves Light green Dark green Shape of stem Gathered in root node, grows, stretches sideways, grows slightly on ground Ground Crawling Number of stems 7-10 15-25 Shape of roots Development of rectus roots extending straight underground Stem crawls to ground and takes root, straight Growth type Early years Single or perennial bloom June June Shape of flowers 3 gunshots, 3 sepals Gunshot Color of flowers White White or pink Arc Sunny Sunny Disease resistance River River Cold resistance River River Growth rate speed speed Ornamental value Leaves are heart-shaped, light green, and when growth is complete, a white oval band is formed in the middle, and when four leaves are gathered, a beautiful shape of a round circle is formed. Leaves are oval, center V-shaped, white or black pattern, no ornamental value

The stems of the new clover 'far' are nodal and grow slightly off the ground, so the total length of the plant was 5 to 10 cm long compared to the wild species, which is ground crawling. Figure 2 shows the growth after planting a new kind of clover 'far' in the pot. The leaves were 4, the length of the leaves was shorter, longer and heart-shaped than the general species. The leaf color of the wild species was dark green, whereas the leaves of the new species 'far' were light green. In addition, when the growth is complete, a white band is formed in the middle of the leaf, and the round shape is formed in the state where four leaves are gathered, so the ornamental value is very excellent. The configuration of the leaves of the new clover 'far' is shown in FIG. 7-10 stems stretched from one laparoscopic node, flowers white, showing three genitalia (Figures 4 and 5). Wild species can be grown for two to three years, while the new breed 'far' is short-lived.

The new species of clover of the present invention can be cultivated under strong light, strong disease resistance, strong when weeds and competition, and because the roots extend straight to the basement, so the growth rate is also fast and grows well without extra fertilizer in the fertilizer. It can also be used for land improvement. In addition, since the leaves are larger than the conventional wild species and the yield is much suitable for feed.

Example 2 Asexual Breeding of a New Clover New Species

Asexual breeding of a new kind of clover of the present invention 'far' was carried out.

As a breeding method, stems and roots were divided and inserted according to a conventional nutrient breeding method at all times except the flowering period of June to August, and the four-leaf clover of the same trait as the mother could be asexually reproduced. In addition, the breeding was fixed, so that the breeding of the oily seed was also possible.

As evident from the above examples, the present invention results in the cultivation of wild leaf white clover consisting of three leaves, four mutant leaves and stems including four leaves, and repeatedly inserting them to repeat asexual growth over five generations. When the growth is complete, four heart-shaped leaves with white oval bands in the middle of the leaves gather to form a round circle, which is highly coronary in value, large in leaves and vigorous for feeding, and excellent for feeding. It is resistant to disease, cold resistance, and competition with weeds, and has a fast growing speed, so it has an excellent effect of providing a new breed of 'clover' and a method of breeding it, which is excellent for grassland and land improvement. It is very useful for other fancy goods manufacturing industry, horticulture industry, livestock feed industry and land improvement industry. To the inventors.

Claims (2)

A cut leaf containing four mutated leaves and stems of 3 to 5 cm from three wild-leaf white clover, three leaves, was first sterilized with 70% ethanol for 3 seconds, and 10% of a commercial bleach (v / v). 2) sterilized by dipping in 20-30 minutes, washed with distilled water and sterilized in 40-45 ℃ for 5 minutes, 5-10 ℃ in cold water for 10 seconds and then BAP (Benzyl-amino-purine) 5.0 After treatment with MG / L, IBA (3-indolebutyric acid) 0.5mg / L, NH ₄ NO ₃ 1650mg / L to increase cell division, humidity 90%, temperature 18-20 ℃, brightness 60%, peat moss Re-select the laps, including the stems of the four-leaf clover, obtained by growing in soil powder containing 1: 1, 1, 1, and 1, and then cutting and cutting the stems or roots. It grows and there are four leaves. When it is finished growing, it is white in the middle of light green heart-shaped leaves. A ring-shaped zone is formed together dog four leaf formed in a circular arc, and light components, cold resistance, and disease resistance is strong, to, characterized in that the growth is vigorous method of clover new variety, so far, it has the characteristics plant breeding. Height: 10-20cm Number of leaves: 4 Leaf shape: Heart-shaped, leaf tip V-shaped Leaf surface: matte and downy Leaf color: light green Stem: Growing at every root, growing sideways and growing slightly on the ground Number of stems: 7-10 Root shape: straight root, beard root development Shape of flower: 3 gunshots, 3 flower stand Flower color: white Breeding type: early spring delete