KR100315161B1 - 섬유소 가수분해능을 갖는 재조합 효모를 이용한재생종이로부터 연료용 에탄올 제조방법 - Google Patents
섬유소 가수분해능을 갖는 재조합 효모를 이용한재생종이로부터 연료용 에탄올 제조방법 Download PDFInfo
- Publication number
- KR100315161B1 KR100315161B1 KR1019990053171A KR19990053171A KR100315161B1 KR 100315161 B1 KR100315161 B1 KR 100315161B1 KR 1019990053171 A KR1019990053171 A KR 1019990053171A KR 19990053171 A KR19990053171 A KR 19990053171A KR 100315161 B1 KR100315161 B1 KR 100315161B1
- Authority
- KR
- South Korea
- Prior art keywords
- yeast
- fuel
- glucose
- ethanol
- recycled paper
- Prior art date
Links
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 title claims abstract description 82
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 29
- 239000000446 fuel Substances 0.000 title claims abstract description 17
- 239000001913 cellulose Substances 0.000 title claims description 11
- 229920002678 cellulose Polymers 0.000 title claims description 11
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 40
- 108090000790 Enzymes Proteins 0.000 claims abstract description 32
- 102000004190 Enzymes Human genes 0.000 claims abstract description 32
- 238000000034 method Methods 0.000 claims abstract description 32
- 238000000855 fermentation Methods 0.000 claims abstract description 31
- 230000004151 fermentation Effects 0.000 claims abstract description 31
- 230000008569 process Effects 0.000 claims abstract description 23
- 102000009123 Fibrin Human genes 0.000 claims abstract description 14
- 108010073385 Fibrin Proteins 0.000 claims abstract description 14
- 229950003499 fibrin Drugs 0.000 claims abstract description 14
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 claims abstract description 13
- 238000006206 glycosylation reaction Methods 0.000 claims abstract description 13
- 239000000758 substrate Substances 0.000 claims abstract description 12
- 238000009825 accumulation Methods 0.000 claims abstract description 5
- 230000003301 hydrolyzing effect Effects 0.000 claims abstract description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 20
- 239000008103 glucose Substances 0.000 claims description 20
- 239000000835 fiber Substances 0.000 claims description 12
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 8
- 230000007062 hydrolysis Effects 0.000 claims description 8
- 238000006460 hydrolysis reaction Methods 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 6
- 239000012153 distilled water Substances 0.000 claims description 5
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 4
- 239000003527 fibrinolytic agent Substances 0.000 claims description 4
- 230000003480 fibrinolytic effect Effects 0.000 claims description 4
- 108010071289 Factor XIII Proteins 0.000 claims description 3
- 239000001569 carbon dioxide Substances 0.000 claims description 3
- 238000006243 chemical reaction Methods 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- GPRLSGONYQIRFK-UHFFFAOYSA-N hydron Chemical compound [H+] GPRLSGONYQIRFK-UHFFFAOYSA-N 0.000 claims description 3
- 235000015097 nutrients Nutrition 0.000 claims description 3
- 230000007812 deficiency Effects 0.000 claims description 2
- 239000012533 medium component Substances 0.000 claims description 2
- 238000002203 pretreatment Methods 0.000 claims description 2
- 239000002609 medium Substances 0.000 claims 3
- 238000005406 washing Methods 0.000 claims 2
- 239000001963 growth medium Substances 0.000 claims 1
- 239000007788 liquid Substances 0.000 claims 1
- 230000001502 supplementing effect Effects 0.000 claims 1
- 239000002994 raw material Substances 0.000 abstract description 8
- 229940088598 enzyme Drugs 0.000 description 27
- 108010059892 Cellulase Proteins 0.000 description 6
- 108010047754 beta-Glucosidase Proteins 0.000 description 6
- 102000006995 beta-Glucosidase Human genes 0.000 description 6
- 229940106157 cellulase Drugs 0.000 description 5
- 238000010353 genetic engineering Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 230000007774 longterm Effects 0.000 description 4
- 230000008901 benefit Effects 0.000 description 3
- 230000013595 glycosylation Effects 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- MWUXSHHQAYIFBG-UHFFFAOYSA-N nitrogen oxide Inorganic materials O=[N] MWUXSHHQAYIFBG-UHFFFAOYSA-N 0.000 description 3
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000006227 byproduct Substances 0.000 description 2
- 239000012527 feed solution Substances 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- FQVLRGLGWNWPSS-BXBUPLCLSA-N (4r,7s,10s,13s,16r)-16-acetamido-13-(1h-imidazol-5-ylmethyl)-10-methyl-6,9,12,15-tetraoxo-7-propan-2-yl-1,2-dithia-5,8,11,14-tetrazacycloheptadecane-4-carboxamide Chemical compound N1C(=O)[C@@H](NC(C)=O)CSSC[C@@H](C(N)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](C)NC(=O)[C@@H]1CC1=CN=CN1 FQVLRGLGWNWPSS-BXBUPLCLSA-N 0.000 description 1
- 102100034035 Alcohol dehydrogenase 1A Human genes 0.000 description 1
- 108010023063 Bacto-peptone Proteins 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 101710112457 Exoglucanase Proteins 0.000 description 1
- 238000012366 Fed-batch cultivation Methods 0.000 description 1
- 101710196208 Fibrinolytic enzyme Proteins 0.000 description 1
- 101000892220 Geobacillus thermodenitrificans (strain NG80-2) Long-chain-alcohol dehydrogenase 1 Proteins 0.000 description 1
- 229920002488 Hemicellulose Polymers 0.000 description 1
- 101000780443 Homo sapiens Alcohol dehydrogenase 1A Proteins 0.000 description 1
- 108090000604 Hydrolases Proteins 0.000 description 1
- 102000004157 Hydrolases Human genes 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000010306 acid treatment Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000003915 air pollution Methods 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000002485 combustion reaction Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000036252 glycation Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 229910000160 potassium phosphate Inorganic materials 0.000 description 1
- 235000011009 potassium phosphates Nutrition 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- XTQHKBHJIVJGKJ-UHFFFAOYSA-N sulfur monoxide Chemical class S=O XTQHKBHJIVJGKJ-UHFFFAOYSA-N 0.000 description 1
- 229910052815 sulfur oxide Inorganic materials 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
Landscapes
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
전처리방법 | 점도 (cP) |
대조군 | 850 |
수도물 | 393 |
약산처리 | 278 |
총셀룰라제(FPU/g-cellulose) | 베타글루코시다제(IU/g-cellulose) | 에탄올(g/L) | 수율(%) | 비효소량 |
10 | 30 | 12.54 | 44.15 | - |
20 | 30 | 16.32 | 57.46 | - |
30 | 30 | 19.94 | 70.21 | 0.66 |
40 | 30 | 19.84 | 69.86 | 0.50 |
50 | 30 | 20.12 | 70.85 | 0.40 |
40 | 10 | 16.36 | 57.61 | - |
40 | 20 | 19.64 | 69.15 | 0.98 |
40 | 30 | 19.84 | 69.86 | 0.66 |
40 | 40 | 19.88 | 70.00 | 0.50 |
40 | 50 | 20.01 | 70.46 | 0.40 |
Claims (6)
- 효모를 이용하여 섬유소(cellulose) 자원으로부터 연료용 에탄올을 제조하는 방법에 있어서,첫째, 상기 섬유소 자원을 증류수로 현탁시키고, 30분간 121℃ 15 psig의 증기압 하에서 1%의 약한 황산용액을 첨가처리하여 점도를 낮춤으로서 효소당화특성을 향상시키는 전처리 단계와,둘째, 상기 섬유소 자원을 최종 수소이온 농도가 4-5가 될 때까지 증류수로세척한 후 상기 섬유소 자원에서 다량의 수분을 필터를 이용하여 제거하고, 60℃ 건조대에서 완전히 건조하는 세척 및 건조 단계와,셋째, 상기 효모를 축적하기 위하여 포도당과 기타 영양소를 포함한 배지를 이용하여 효모를 접종한 반응기에서 약 50% 부피까지 회분식 배양을 한 후, 20%의 포도당과 상기 회분식 배양에서 사용한 반복합 최소배지 성분의 2배를 첨가한 액을 공급하여 유가식 배양을 하여 효모와 효소를 축적하는 단계와,넷째, 상기한 효모의 증식과 효소의 추가적 축적이 종료되는 시기에 유가식 배양을 중지하고, 최적의 효소량을 위한 부족분을 상용효소를 첨가하여 보충하는 단계와,다섯째, 기질인 상기 섬유소 자원을 최종농도가 100 g/L 이상이 되도록 상기 배지에 공급하여 동시당화발효공정을 수행하는 단계로 이루어진 것을 특징으로 하는 섬유소 가수분해능을 갖는 재조합 효모를 이용한 재생종이로부터 연료용 에탄올 제조방법.
- 제 1 항에 있어서,상기 섬유소 자원을 동시당화발효공정의 고농도 기질로 혼합 재생종이를 사용하는 것을 특징으로 섬유소 가수분해능을 갖는 재조합 효모를 이용한 재생종이로부터 연료용 에탄올 제조방법.
- 제 1 항에 있어서,상기 효모의 배양에서 포도당이 2% 포함된 반복합 최소배지에서 회분식 및 유가식으로 배양하여 배양액이 섬유소 가수분해능을 갖는 것을 특징으로 하는 섬유소 가수분해능을 갖는 재조합 효모를 이용한 재생종이로부터 연료용 에탄올 제조방법.
- 제 1 항에 있어서,상기 회분식 배양에서 유가식 배양으로의 전환 및 기질공급 시기의 결정은 실시간 확인하는 이산화탄소 발생량이 최고에 달하는 시점에 결정되는 것을 특징으로 하는 섬유소 가수분해능을 갖는 재조합 효모를 이용한 재생종이로부터 연료용 에탄올 제조방법.
- 제 1 항에 있어서,상기 유가식 배양시에 재조합 효모에 의한 포도당의 소모속도를 계산하고 포도당 분석기를 이용한 실제분석을 통해 얻은 포도당 농도를 이용하여 섬유소 분해효소의 최적발현을 위한 포도당 농도를 유지할 수 있도록 포도당을 공급하는 것을 특징으로 하는 섬유소 가수분해능을 갖는 재조합 효모를 이용한 재생종이로부터 연료용 에탄올 제조방법.
- 제 1 항에 있어서,상기 본 발명의 공정이 효모의 생리적 최적온도인 30℃에서 조업함으로써 많은 에너지 비용의 절감 및 효율의 향상을 갖는 것을 특징으로 하는 섬유소 가수분해능을 갖는 재조합 효모를 이용한 재생종이로부터 연료용 에탄올 제조방법.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1019990053171A KR100315161B1 (ko) | 1999-11-26 | 1999-11-26 | 섬유소 가수분해능을 갖는 재조합 효모를 이용한재생종이로부터 연료용 에탄올 제조방법 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1019990053171A KR100315161B1 (ko) | 1999-11-26 | 1999-11-26 | 섬유소 가수분해능을 갖는 재조합 효모를 이용한재생종이로부터 연료용 에탄올 제조방법 |
Publications (2)
Publication Number | Publication Date |
---|---|
KR20010048483A KR20010048483A (ko) | 2001-06-15 |
KR100315161B1 true KR100315161B1 (ko) | 2001-11-26 |
Family
ID=19622169
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019990053171A KR100315161B1 (ko) | 1999-11-26 | 1999-11-26 | 섬유소 가수분해능을 갖는 재조합 효모를 이용한재생종이로부터 연료용 에탄올 제조방법 |
Country Status (1)
Country | Link |
---|---|
KR (1) | KR100315161B1 (ko) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR101254824B1 (ko) * | 2004-11-29 | 2013-04-15 | 코벤하븐스 유니벌스테 | 높은 건조물 (dm) 함량을 갖는 바이오매스의 효소적가수분해 |
KR20200005776A (ko) | 2018-07-09 | 2020-01-17 | 국민대학교산학협력단 | 에탄올 및 c4-유기산 생성용 재조합 효모 |
-
1999
- 1999-11-26 KR KR1019990053171A patent/KR100315161B1/ko active IP Right Grant
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR101254824B1 (ko) * | 2004-11-29 | 2013-04-15 | 코벤하븐스 유니벌스테 | 높은 건조물 (dm) 함량을 갖는 바이오매스의 효소적가수분해 |
KR20200005776A (ko) | 2018-07-09 | 2020-01-17 | 국민대학교산학협력단 | 에탄올 및 c4-유기산 생성용 재조합 효모 |
Also Published As
Publication number | Publication date |
---|---|
KR20010048483A (ko) | 2001-06-15 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Eklund et al. | Simultaneous saccharification and fermentation of steam-pretreated willow | |
Chandel et al. | The realm of cellulases in biorefinery development | |
Reczey et al. | Cellulase production by T. reesei | |
Tosuner et al. | Effects of rice husk particle size on biohydrogen production under solid state fermentation | |
DK2744899T3 (en) | PROCEDURE FOR PREPARING CELLULASES FROM A FILAMENT'S FUNGI ADAPTED TO A SLOW VOLUMETRIC OXYGEN TRANSFER COFFEE EFFICIENCY CLASS | |
Liu et al. | Construction of consolidated bio-saccharification biocatalyst and process optimization for highly efficient lignocellulose solubilization | |
Wayman et al. | Bioconversion of waste paper to ethanol | |
Han et al. | Production of sodium gluconate from delignified corn cob residue by on-site produced cellulase and co-immobilized glucose oxidase and catalase | |
An et al. | Biological saccharification by Clostridium thermocellum and two-stage hydrogen and methane production from hydrogen peroxide-acetic acid pretreated sugarcane bagasse | |
Zagrodnik et al. | Direct fermentative hydrogen production from cellulose and starch with mesophilic bacterial consortia | |
Lu et al. | Optimization of biohydrogen production from cornstalk through surface response methodology | |
AU2012322594B2 (en) | Method for the continuous production of cellulases by a filamentous fungus using a carbon substrate obtained from an acid pretreatment | |
AU2013279186B2 (en) | Method for producing an enzyme cocktail using the liquid residue from a method for biochemically converting lignocellulosic materials | |
Iram et al. | Optimization of the fermentation parameters to maximize the production of cellulases and xylanases using DDGS as the main feedstock in stirred tank bioreactors | |
KR100315161B1 (ko) | 섬유소 가수분해능을 갖는 재조합 효모를 이용한재생종이로부터 연료용 에탄올 제조방법 | |
CN101784678A (zh) | 用于纤维素糖化的方法和反应器 | |
CN102031276A (zh) | 一种生物、碱复合预处理秸秆纤维原料两步发酵产氢的方法 | |
CN107299120B (zh) | 一种提高厌氧细菌产丁醇活性的方法 | |
Mohagheghi et al. | Production of cellulase on mixtures of xylose and cellulose in a fed‐batch process | |
CN114807098B (zh) | 用于生产胞外纤维素降解酶系的培养方法 | |
CN111334447A (zh) | 高产纤维素酶的梭热杆菌的发酵工艺 | |
CN105734095B (zh) | 一种提高纤维素酶解的方法 | |
WO2013122549A1 (en) | A method for enzymatic hydrolysis of cellulose | |
CN106591381B (zh) | 一种利用海带渣连续预处理制备生物乙醇的方法 | |
Florenzano et al. | A study of acetate production from cellulose using Clostridium thermocellum |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A201 | Request for examination | ||
E701 | Decision to grant or registration of patent right | ||
GRNT | Written decision to grant | ||
FPAY | Annual fee payment |
Payment date: 20121108 Year of fee payment: 12 |
|
FPAY | Annual fee payment |
Payment date: 20131107 Year of fee payment: 13 |
|
FPAY | Annual fee payment |
Payment date: 20141105 Year of fee payment: 14 |
|
FPAY | Annual fee payment |
Payment date: 20151105 Year of fee payment: 15 |
|
FPAY | Annual fee payment |
Payment date: 20161107 Year of fee payment: 16 |
|
FPAY | Annual fee payment |
Payment date: 20171106 Year of fee payment: 17 |
|
FPAY | Annual fee payment |
Payment date: 20181105 Year of fee payment: 18 |
|
FPAY | Annual fee payment |
Payment date: 20191105 Year of fee payment: 19 |